Automatic tissue gradient dehydration transparentizing device

Abstract

The utility model discloses an automatic tissue gradient dehydration transparentizing device, and relates to the technical field of biomedicine, in particular to the automatic tissue gradient dehydration transparentizing device which comprises a base frame and a net-shaped barrel, and the inner bottom wall of the base frame is provided with a first liquid box, a second liquid box, a two-way pump and an electric push rod. A rectangular frame is installed at the movable end of the electric push rod, and a servo motor is installed in the rectangular frame. The automatic tissue gradient dehydration transparentizing device has the effect of conveniently and automatically moving to dehydrate biological tissues, through the arrangement of an electric push rod and a net-shaped barrel, the biological tissues are conveniently soaked in a first solution box and a second solution box, and through the cooperation of a threaded rod and a threaded ring block, the biological tissues can be conveniently soaked in the first solution box and the second solution box; the biological tissue can be conveniently soaked, dehydrated and transparent in alcohol and xylene solutions with different concentrations, the manual operation is reduced, the working efficiency is improved, and the purpose of improving the practicability is achieved.

Description

Technical Field

[0001] This utility model relates to the field of biomedical technology, specifically to an automatic tissue gradient dehydration and transparency device. Background Technology

[0002] Immunohistochemistry and hematoxylin and eosin (HE) staining are key techniques in pathological examination, and the processing of biological tissues is a crucial first step that directly determines the final microscopic tissue image. Fresh tissues need to be fixed with paraformaldehyde and then soaked in alcohol solutions of progressively increasing concentrations for a certain period of time to achieve a gradient dehydration effect. Afterward, the tissues are soaked in xylene solution to clear them, which facilitates the final penetration of paraffin into the tissue.

[0003] However, most laboratory operators currently need to manually pour pre-prepared alcohol and xylene solutions of different concentrations into the corresponding dehydration boxes, and then manually transfer the tissue to the next solution after the allotted time. After each use, the solutions need to be recycled. This process consumes a lot of time and effort, especially since xylene solution is toxic and harmful to the operators. While there are automated tissue dehydration machines on the market, they are expensive and only a few laboratories purchase them. Most still rely on manual dehydration and clearing by operators. Now, an automated tissue gradient dehydration and clearing device has been invented to solve the above problems. Utility Model Content

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides an automatic tissue gradient dehydration and transparency device, which solves the problems mentioned in the background section.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, this utility model is implemented through the following technical solution: an automatic gradient dehydration and transparency device, comprising a base frame and a mesh bucket. The inner bottom wall of the base frame is respectively equipped with a first liquid tank, a second liquid tank, a bidirectional pump, and an electric push rod. The movable end of the electric push rod is equipped with a rectangular frame. A servo motor is installed inside the rectangular frame. The output end of the servo motor is equipped with a threaded rod whose axis is co-centered with the output end of the servo motor. A threaded ring block is connected to the outer surface of the threaded rod. Rectangular sliders are installed at the top and bottom of the threaded ring block. A steel cable is installed at the bottom of the threaded ring block. A hook is installed at the end of the steel cable. Fixing blocks are installed on both sides of the hook. A baffle is rotatably connected to one side of the fixing block via a shaft. A return spring is installed behind the baffle. A handle is provided at the top of the mesh bucket. A first connecting pipe and a second connecting pipe are respectively provided at both ends of the bidirectional pump.

[0008] Optionally, the first liquid tank and the second liquid tank are the same size, the diameter of the mesh bucket is smaller than the width of the first liquid tank, and the position of the mesh bucket corresponds to the position of the first liquid tank and the second liquid tank, respectively.

[0009] Optionally, the end of the threaded rod away from the servo motor is rotatably connected to the inner wall of the rectangular frame via a bearing, and the positions of the first liquid tank and the second liquid tank correspond to each other.

[0010] Optionally, the rectangular frame has a rectangular groove inside, with the vertical direction as the depth direction and the horizontal direction as the length direction. The end of the rectangular slider is located inside the rectangular groove, and the rectangular slider can slide along the length direction of the rectangular groove.

[0011] Optionally, the end of the baffle abuts against the inner wall of the hook, the end of the return spring away from the baffle is fixedly connected to the hook, and the handle hook is hung on the hook.

[0012] Optionally, there are several first liquid tanks and several second liquid tanks, with the end of the first connecting pipe away from the bidirectional pump located inside the first liquid tank, and the end of the second connecting pipe away from the bidirectional pump located inside the second liquid tank.

[0013] This invention provides an automatic tissue gradient dehydration and transparency device, which has the following beneficial effects:

[0014] 1. This automatic tissue gradient dehydration and transparency device, through the arrangement of an electric push rod, a rectangular frame, a servo motor, a threaded rod, a threaded ring block, and a rectangular slider, enables convenient automatic movement for dehydrating biological tissues. The electric push rod and mesh tank facilitate the immersion of biological tissues in the first and second solution tanks. The cooperation of the threaded rod and threaded ring block allows for easy immersion and dehydration of biological tissues in alcohol and xylene solutions of different concentrations, reducing manual operation, improving work efficiency, and achieving the goal of enhancing practicality.

[0015] 2. This automatic tissue gradient dehydration and transparency device, through the design of hooks, fixing blocks, baffles, return springs, and handles, facilitates the placement and removal of the mesh bucket. The hooks hang the mesh bucket, the baffles improve the stability of the mesh bucket, and the mesh bucket is easy to disassemble and place. The bidirectional pump facilitates the exchange of solutions in the first and second liquid tanks, reducing the contact of workers with the toxic reagent xylene. This device is inexpensive, has a wide range of applications, and achieves the goal of improving practicality. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the three-dimensional oblique structure of this utility model;

[0017] Figure 2This utility model Figure 1 Enlarged structural diagram at point A;

[0018] Figure 3 This is a side view sectional diagram of the present invention.

[0019] Figure 4 This is a top view sectional diagram of the present invention.

[0020] Figure 5 This is a top view of a partial cross-section of the present invention.

[0021] In the diagram: 1. Base frame; 2. Mesh tank; 3. First liquid tank; 4. Second liquid tank; 5. Bidirectional pump; 6. Electric push rod; 7. Rectangular frame; 8. Servo motor; 9. Threaded rod; 10. Threaded ring block; 11. Rectangular slider; 12. Steel cable; 13. Hook; 14. Fixing block; 15. Baffle; 16. Return spring; 17. Handle; 18. First connecting pipe; 19. Second connecting pipe. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0023] Example 1

[0024] Please see Figures 1 to 5 This utility model provides a technical solution: an automatic tissue gradient dehydration and transparency device, including a base frame 1 and a mesh bucket 2. The inner bottom wall of the base frame 1 is respectively equipped with a first liquid tank 3, a second liquid tank 4, a bidirectional pump 5, and an electric push rod 6. The movable end of the electric push rod 6 is equipped with a rectangular frame 7. A servo motor 8 is installed inside the rectangular frame 7. The output end of the servo motor 8 is equipped with a threaded rod 9 with the same center as the output end axis of the servo motor 8. The outer surface of the threaded rod 9 is connected to a threaded ring block 10. A rectangular slider 11 is installed at the top and bottom of the threaded ring block 10. A steel cable 12 is installed at the bottom of the threaded ring block 10. A hook 13 is installed at the end of the steel cable 12. Fixing blocks 14 are installed on both sides of the hook 13. A baffle 15 is rotatably connected to one side of the fixing block 14 through a shaft. A return spring 16 is installed behind the baffle 15. A handle 17 is provided at the top of the mesh bucket 2. A first connecting pipe 18 and a second connecting pipe 19 are respectively provided at both ends of the bidirectional pump 5.

[0025] Specifically, the mesh design of the mesh bucket 2 allows the biological tissue inside the mesh bucket 2 to be better immersed in alcohol and xylene.

[0026] Please see Figures 1 to 5The first liquid tank 3 and the second liquid tank 4 are the same size. The diameter of the mesh bucket 2 is smaller than the width of the first liquid tank 3. The position of the mesh bucket 2 corresponds to the position of the first liquid tank 3 and the second liquid tank 4, respectively.

[0027] Specifically, the threaded rod 9 is rotatably connected to the inner wall of the rectangular frame 7, which improves the stability of the rotation of the threaded rod 9. The positions of the first liquid tank 3 and the second liquid tank 4 correspond to each other, so that alcohol and xylene of the same concentration correspond.

[0028] Please see Figures 4 to 5 The end of the threaded rod 9 away from the servo motor 8 is rotatably connected to the inner wall of the rectangular frame 7 through a bearing, and the positions of the first liquid tank 3 and the second liquid tank 4 correspond to each other.

[0029] Specifically, the rectangular slider 11 slides along the length of the rectangular groove to improve the stability of the movement of the threaded ring block 10 and the mesh barrel 2.

[0030] Please see Figure 4 The rectangular frame 7 has a rectangular groove inside with the vertical direction as the depth direction and the horizontal direction as the length direction. The end of the rectangular slider 11 is located inside the rectangular groove, and the rectangular slider 11 can slide along the length direction of the rectangular groove.

[0031] Specifically, the baffle 15 restricts the position of the handle 17, improves the stability of the mesh bucket 2, and prevents the mesh bucket 2 from falling off.

[0032] Please see Figures 1 to 2 The end of the baffle 15 abuts against the inner wall of the hook 13, the end of the return spring 16 away from the baffle 15 is fixedly connected to the hook 13, and the handle 17 is hooked on the hook 13.

[0033] Specifically, the first liquid tank 3 and the second liquid tank 4, which contain different concentrations of alcohol and xylene, are placed in sequence to facilitate the sequential immersion of biological tissues in alcohol and xylene of different concentrations. The bidirectional pump 5 facilitates the exchange of alcohol and xylene in the first liquid tank 3 and the second liquid tank 4.

[0034] Please see Figures 1 to 5 There are several first liquid tanks 3 and second liquid tanks 4. The end of the first connecting pipe 18 away from the bidirectional pump 5 is located inside the first liquid tank 3, and the end of the second connecting pipe 19 away from the bidirectional pump 5 is located inside the second liquid tank 4.

[0035] In use, alcohol is poured into the first liquid tank 3, and xylene is poured into the second liquid tank 4, allowing the biological tissue to soak in alcohol and xylene of different concentrations. The biological tissue is then placed inside the mesh bucket 2, and the handle 17 on the mesh bucket 2 is hooked onto the hook 13. The handle 17 abuts against the baffle 15, causing the baffle 15 to rotate at the rotatable connection between the baffle 15 and the fixing block 14. This causes the return spring 16 to contract under force, allowing the handle 17 to hook onto the hook 13. The return spring 16 then contracts due to its elastic tension. The end of the baffle 15 abuts against the hook 13 to restrict the position of the handle 17 and prevent the mesh bucket 2 from falling off. The movable end of the electric push rod 6 retracts, causing the rectangular frame 7, threaded ring block 10, steel cable 12, hook 13, and mesh bucket 2 to move downwards, immersing the biological tissue inside the mesh bucket 2 in alcohol and xylene. The mesh design of the mesh bucket 2 facilitates better immersion of the biological tissue in alcohol and xylene. After immersion for a certain period of time, the movable end of the electric push rod 6 extends, raising and resetting the mesh bucket 2, suspending it. After standing for 30-60 seconds, drain the residual solution from the mesh bucket 2 and the tissue. After standing, the servo motor 8 drives the threaded rod 9 to rotate. The threaded rod 9 is connected to the threaded ring block 10, which in turn drives the rectangular slider 11, the steel cable 12, and the mesh bucket 2 to move. The rectangular slider 11 slides along the length of the rectangular groove, moving the mesh bucket 2 above the next first liquid tank 3 or second liquid tank 4. Repeat the above steps to immerse the biological tissue in the corresponding first liquid tank 3 or second liquid tank 4, facilitating dehydration and transparency of the biological tissue. The bidirectional pump 5 allows the solution in the first liquid tank 3 to be pumped into the second liquid tank 4 through the first connecting pipe 18 and the second connecting pipe 19, and vice versa. The bidirectional pump 5 eliminates the need for a recovery step, reducing the operator's contact with the toxic reagent xylene. The device is simple in structure and inexpensive, making it affordable for most laboratories while improving the operator's experimental efficiency.

[0036] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An automatic tissue gradient dehydration and transparency device, comprising a base frame and a mesh barrel, characterized in that: The inner bottom wall of the base frame is equipped with a first liquid tank, a second liquid tank, a bidirectional pump, and an electric push rod. A rectangular frame is installed at the movable end of the electric push rod. A servo motor is installed inside the rectangular frame. A threaded rod with the same center as the output shaft of the servo motor is installed at the output end of the servo motor. A threaded ring block is connected to the outer surface of the threaded rod. A rectangular slider is installed at the top and bottom of the threaded ring block. A steel cable is installed at the bottom of the threaded ring block. A hook is installed at the end of the steel cable. Fixing blocks are installed on both sides of the hook. A baffle is rotatably connected to one side of the fixing block via a shaft. A return spring is installed behind the baffle. A handle is provided at the top of the mesh tank. A first connecting pipe and a second connecting pipe are respectively provided at both ends of the bidirectional pump.

2. The automatic tissue gradient dehydration and transparency device according to claim 1, characterized in that: The first liquid tank and the second liquid tank are the same size. The diameter of the mesh bucket is smaller than the width of the first liquid tank. The position of the mesh bucket corresponds to the position of the first liquid tank and the second liquid tank, respectively.

3. The automatic tissue gradient dehydration and transparency device according to claim 1, characterized in that: The end of the threaded rod away from the servo motor is rotatably connected to the inner wall of the rectangular frame via a bearing, and the positions of the first liquid tank and the second liquid tank correspond to each other.

4. The automatic tissue gradient dehydration and transparency device according to claim 1, characterized in that: The rectangular frame has a rectangular groove inside, with the vertical direction as the depth direction and the horizontal direction as the length direction. The end of the rectangular slider is located inside the rectangular groove, and the rectangular slider can slide along the length direction of the rectangular groove.

5. The automatic tissue gradient dehydration and transparency device according to claim 1, characterized in that: The end of the baffle abuts against the inner wall of the hook, the end of the return spring away from the baffle is fixedly connected to the hook, and the handle hook is hung on the hook.

6. The automatic tissue gradient dehydration and transparency device according to claim 1, characterized in that: There are several first liquid tanks and several second liquid tanks. The end of the first connecting pipe away from the bidirectional pump is located inside the first liquid tank, and the end of the second connecting pipe away from the bidirectional pump is located inside the second liquid tank.

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