Chromatographic column sample injection pipeline and system

Abstract

The utility model discloses a chromatographic column sample introduction pipeline and system relates to chromatographic column sample introduction pipe field, including chromatographic column and set up the transition pipe at chromatographic column top end, the transition pipe top end is communicated with the liquid inlet pipeline, the transition pipe bottom end is communicated with the liquid outlet, the liquid outlet extends into the chromatographic column, and the liquid inlet pipeline is communicated with the liquid outlet. A lifting floating plate is arranged in the transition pipe in a lifting mode, a supporting ring is fixed in the transition pipe, through the synergistic effect of the lifting floating plate, a threaded rod and a sealing block, when the chromatographic column is filled with liquid, a liquid outlet can be sealed in time, liquid overflowing is effectively avoided, and the cleanliness of the experiment environment and the experiment safety are guaranteed; meanwhile, the lifting floating plate ascends and descends in the transition pipe and is limited by the inner wall of the transition pipe, and the floating stability of the sealing block is improved. The supporting ring is used for limiting the descending position of the lifting floating plate and preventing the lifting floating plate from being over away from the bottom end of the transition pipe to affect normal work.

Description

Technical Field

[0001] This utility model relates to the field of chromatographic column injection tubes, and in particular to a chromatographic column injection pipe and system. Background Technology

[0002] Preparative liquid chromatography (PLC) is a highly efficient separation technique for obtaining pure samples. It originated in the 1930s and was initially used for the separation of pigments. Since then, it has been developed significantly and is now widely used in the pharmaceutical industry, protein separation and purification, such as the separation and purification of secondary metabolites, natural products, macromolecular compounds, and certain important pharmaceutical bioactive proteins, biotherapeutic products, and diagnostic reagents.

[0003] For example, a patent entitled "Chromatographic Column Injection System" (patent application number: CN202022413075.6) discloses a chromatographic column injection system. The system effectively prevents liquid from overflowing from the transition structure when it is full by using the concave surface of the stop block and the convex ball of the liquid inlet pipe. However, the stop block itself does not have a guiding mechanism to guide its ascent. After the liquid drives the stop block to float up, the flow of the liquid can easily cause the stop block to shake and tilt, which can easily lead to the concave surface of the stop block not being able to contact the liquid outlet.

[0004] Therefore, it is necessary to propose a chromatographic column injection pipeline and system to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide a chromatographic column injection pipe and system to solve the problem that the stop block itself does not have a guiding mechanism to guide its ascent. After the liquid drives the stop block to float, the flow of the liquid easily causes the stop block to shake and tilt, which can easily lead to the concave surface of the stop block not being able to contact the liquid outlet.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a chromatographic column injection pipe, comprising a chromatographic column and a transition tube disposed at the top of the chromatographic column, wherein the top of the transition tube is connected to an inlet pipe and the bottom of the transition tube is connected to an outlet, the outlet extending into the interior of the chromatographic column;

[0007] A lifting float is installed inside the transition tube, and a support ring is fixed inside the transition tube. The support ring is located below the lifting float, and filter holes are opened on the lifting float.

[0008] A threaded rod is provided in the middle of the lifting float, and a sealing block is fixedly connected to the top of the threaded rod. The sealing block is located directly below the bottom of the liquid inlet pipe and is a downwardly concave hemispherical shape.

[0009] Preferably, the top notch of the sealing block corresponds to the bottom end of the liquid inlet pipe, and an elastic buffer pad is fixedly connected inside the notch;

[0010] The inlet pipe has a hemispherical convex sphere in the middle.

[0011] Preferably, a sealing ring is provided at the top of the inside of the chromatographic column, and the liquid outlet extends into the bottom end of the sealing ring.

[0012] Preferably, a sieve plate is fixed at the bottom of the chromatographic column, and packing material is provided on the sieve plate.

[0013] Preferably, the lifting float has a threaded hole for the threaded rod to pass through, the bottom end of the threaded rod passes through the lifting float, and a nut is threaded onto the end of the threaded rod.

[0014] Preferably, the enclosed block has a cavity inside, and the cavity contains a light gas.

[0015] Preferably, the bottom end of the chromatographic column is connected to an outlet.

[0016] This utility model also discloses a chromatographic column injection system, including the above-mentioned chromatographic column injection pipe.

[0017] The technical effects and advantages of this utility model are as follows:

[0018] 1. When the liquid enters the transition tube, the impact force of the liquid will cause the lifting float to descend and stop descending on the support ring. The liquid can then enter the chromatographic column through the filter holes, while impurities in the liquid will be blocked by the filter holes.

[0019] 2. Through the coordinated action of the lifting float, threaded rod, and sealing block, the outlet can be sealed promptly when the column is full of liquid, effectively preventing liquid overflow, ensuring a clean experimental environment and experimental safety, reducing sample waste and instrument contamination. Simultaneously, the lifting float moves up and down in the transition tube, and is limited by the inner wall of the transition tube, improving the stability of the sealing block's upward movement. The support ring restricts the descent position of the lifting float, preventing it from moving too far from the bottom of the transition tube and affecting normal operation.

[0020] 3. The bottom end of the threaded rod passes through the lifting float, and a nut is threaded onto the end of the rod. By adjusting the position of the nut on the threaded rod, the height of the threaded rod and the sealing block can be changed, thereby adapting to different working requirements and improving the flexibility and applicability of the device. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the chromatographic column injection pipe of this utility model.

[0022] Figure 2 This utility model Figure 1 Enlarged diagram of point A in the middle.

[0023] In the diagram: 1. Chromatographic column; 2. Inlet pipe; 3. Convex ball; 4. Transition tube; 5. Sealing ring; 6. Sieve plate; 7. Outlet; 8. Nut; 9. Support ring; 10. Lifting float; 11. Filter hole; 12. Threaded rod; 13. Sealing block. Detailed Implementation

[0024] This utility model provides, for example Figures 1-2 The illustrated chromatographic column injection channel includes a chromatographic column 1 and a transition tube 4 disposed at the top of the chromatographic column 1. The top of the transition tube 4 is connected to an inlet pipe 2, and the bottom of the transition tube 4 is connected to an outlet 7, which extends into the interior of the chromatographic column 1. The top of the inlet pipe 2 serves as the material inlet. The inlet pipe 2 and the transition tube 4 form a channel for the sample to enter the interior of the chromatographic column 1 from the inlet pipe.

[0025] A lifting float 10 is installed inside the transition tube 4. A support ring 9 is fixed inside the transition tube 4. The support ring 9 is located below the lifting float 10. Filter holes 11 are opened on the lifting float 10.

[0026] When the liquid enters the transition tube 4, the impact force of the liquid will cause the lifting float 10 to descend and fall onto the support ring 9, where it will stop descending. The liquid can then enter the chromatographic column 1 through the filter hole 11, while impurities in the liquid will be blocked by the filter hole 11.

[0027] Once the column 1 is filled with liquid, it enters the transition tube 4. The liquid causes the lifting float 10 to rise, which in turn causes the concave side of the sealing block 13 to press against the outlet 7, preventing liquid overflow. Simultaneously, the lifting float 10 moves up and down within the transition tube 4, and is confined by the inner wall of the transition tube 4, improving the stability of the sealing block 13's upward movement. The support ring 9 restricts the descent of the lifting float 10, preventing it from moving too far from the bottom of the transition tube 4 and affecting normal operation.

[0028] A threaded rod 12 is provided in the middle of the lifting float 10. A sealing block 13 is fixedly connected to the top of the threaded rod 12. The sealing block 13 is located directly below the bottom of the liquid inlet pipe 2 and is a downwardly concave hemispherical shape.

[0029] The top notch of the sealing block 13 corresponds to the bottom end of the liquid inlet pipe 2, and an elastic buffer pad is fixedly connected inside the notch; this makes the sealing block 13 fit more tightly with the bottom end of the liquid inlet pipe 2, and the elastic buffer pad can also reduce the impact force when the two come into contact, while enhancing the sealing performance.

[0030] The middle part of the liquid inlet pipe 2 has a hemispherical convex sphere 3.

[0031] A sealing ring 5 is provided at the top of the inside of the chromatographic column 1, and the outlet 7 extends into the bottom of the sealing ring 5.

[0032] A sieve plate 6 is fixed at the bottom of the chromatographic column 1, and packing material is placed on the sieve plate 6. The sieve plate 6 can support the packing material, prevent packing material loss, and at the same time allow the sample to pass through the packing material uniformly, ensuring the chromatographic separation effect.

[0033] The lifting float 10 has a threaded hole for the threaded rod 12 to pass through. The bottom end of the threaded rod 12 passes through the lifting float 10, and a nut 8 is threaded onto the end through which it passes. By adjusting the position of the nut 8 on the threaded rod 12, the height of the threaded rod 12 and the closing block 13 can be changed, thereby adapting to different working requirements and improving the flexibility and applicability of the device.

[0034] The enclosed block 13 has an internal cavity filled with a lightweight gas. The presence of the lightweight gas reduces the weight of the enclosed block 13, making it easier for the lifting float 10 to lift the enclosed block 13, thus improving the sensitivity and response speed of the entire device.

[0035] The bottom of column 1 is connected to an outlet.

[0036] This utility model also discloses a chromatographic column injection system, including the above-mentioned chromatographic column injection pipe.

Claims

1. A chromatographic column sample inlet conduit, comprising a chromatographic column (1) and a transition tube (4) arranged at the top end of the chromatographic column (1), and a liquid inlet conduit (2) communicating with the top end of the transition tube (4), characterized in that: The bottom end of the transition tube (4) is connected to the liquid outlet (7), which extends into the interior of the chromatographic column (1); A lifting float (10) is installed inside the transition tube (4), and a support ring (9) is fixed inside the transition tube (4). The support ring (9) is located below the lifting float (10), and filter holes (11) are opened on the lifting float (10). A threaded rod (12) is provided in the middle of the lifting float (10), and a sealing block (13) is fixedly connected to the top of the threaded rod (12). The sealing block (13) is located directly below the bottom of the liquid inlet pipe (2), and the sealing block (13) is set as a downwardly concave hemispherical shape.

2. A chromatographic column sample feed conduit according to claim 1, wherein: The top notch of the sealing block (13) corresponds to the bottom end of the liquid inlet pipe (2), and an elastic buffer pad is fixedly connected inside the notch; The liquid inlet pipe (2) has a hemispherical convex sphere (3) in the middle.

3. A chromatographic column sample feed conduit according to claim 1, wherein: The chromatographic column (1) is provided with a sealing ring (5) at the top inside, and the liquid outlet (7) extends into the bottom end of the sealing ring (5).

4. A chromatographic column sample feed conduit according to claim 1, wherein: The chromatographic column (1) has a sieve plate (6) fixed at the bottom inside, and the sieve plate (6) is provided with packing material.

5. A chromatographic column sample feed conduit according to claim 1 wherein: The lifting float (10) has a threaded hole for the threaded rod (12) to pass through. The bottom end of the threaded rod (12) passes through the lifting float (10), and a nut (8) is threaded onto the end of the rod.

6. A chromatographic column sample feed conduit according to claim 1 wherein: The enclosed block (13) has a cavity inside, and the cavity contains a light gas.

7. A chromatographic column sample feed conduit according to claim 1 wherein: The bottom end of the chromatographic column (1) is connected to an outlet.

8. A chromatographic column sample introduction system characterized by, Includes the chromatographic column injection tubing as described in any one of claims 1-7.

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