cryopreservation tube assembly

Abstract

This disclosure relates to the field of cryopreservation technology for biological samples, and in particular to a cryopreservation tube assembly, comprising a cap, a tube body, and a carrier. The cap is detachably connected to the tube body, and the carrier is connected to the cap. The carrier has a bearing surface for holding a target sample, and the bearing surface is provided with a through hole for positioning the target sample. The bearing surface can extend into the tube body to place the target sample inside the tube. In use, the target sample is placed at the position of the through hole, allowing the target sample to fully contact the freezing medium, thus improving the freezing rate. Furthermore, it also improves the thawing rate during thawing, making the target sample easier to detach, which is beneficial for maintaining the sample's recovery rate and viability.

Description

Technical Field

[0001] This disclosure relates to the field of cryopreservation technology for biological samples, and in particular to a cryopreservation tube assembly. Background Technology

[0002] In fields requiring cryopreservation of biological samples such as gametes, zygotes, or embryos, cryovial assemblies are one of the primary tools used to freeze target samples. The structure of cryovial assemblies needs further improvement to enhance freezing and thawing rates, and to maintain sample recovery rates and viability. Utility Model Content

[0003] The purpose of this disclosure is to provide a cryopreservation tube assembly to improve freezing and thawing rates, and to maintain sample recovery rate and viability.

[0004] To achieve the above objectives, this disclosure provides a cryopreservation tube assembly, including a cap, a tube body, and a carrier. The cap is detachably connected to the tube body, and the carrier is connected to the cap. The carrier has a bearing surface for bearing a target sample, and the bearing surface is provided with a through hole for positioning the target sample. The bearing surface can extend into the tube body to place the target sample inside the tube body.

[0005] In one embodiment of this disclosure, the number of through holes is one; and / or, the through hole is a circular hole with a diameter of 0.2 to 0.7 mm.

[0006] In one embodiment of this disclosure, the support member includes a rod and a plate, one end of the rod is connected to the cap, the other end of the rod is connected to the plate, and the support surface is the plate surface of the plate.

[0007] In one embodiment of this disclosure, the thickness of the plate portion is 0.05 to 0.25 mm.

[0008] In one embodiment of this disclosure, the plate portion is a flat plate, or the cross-sectional shape of the plate portion is arc-shaped, V-shaped, wavy, trapezoidal, or concave.

[0009] In one embodiment of this disclosure, the cryopreservation tube assembly further includes a counterweight, which is installed at the end of the tube body away from the tube cap.

[0010] In one embodiment of this disclosure, the tube body is provided with a partition for dividing the internal space of the tube body into a first space and a second space, the first space for accommodating the target sample, and the counterweight being installed in the second space.

[0011] In one embodiment of this disclosure, the counterweight is provided with a discharge hole that extends through the counterweight along the axial direction of the tube.

[0012] In one embodiment of this disclosure, the counterweight has a flow guide on its surface facing the partition, the flow guide being used to guide at least a portion of the refrigerant in the second space to the discharge port.

[0013] In one embodiment of this disclosure, at least one of the counterweight and the tube is provided with a protrusion, the protrusion being used to restrict the axial movement of the counterweight along the tube.

[0014] In one embodiment of this disclosure, the protrusion is disposed on the inner wall of the end of the tube away from the cap, and the protrusion abuts against the surface of the counterweight away from the cap.

[0015] In one embodiment of this disclosure, the counterweight is interference-fitted with the tube body via the protrusion; the protrusion is disposed on the circumferential sidewall of the counterweight, and / or, the protrusion is disposed on the inner wall of the tube body at the end away from the cap.

[0016] In one embodiment of this disclosure, the inner wall of the tube body is provided with a connecting groove. Along the extending direction of the connecting groove, the connecting groove has a first end and a second end. The first end of the connecting groove penetrates the end face of the tube body facing the tube cap, and the second end of the connecting groove is located inside the tube body. The connecting groove is configured such that the speed at which the freezing medium flows from the first end to the second end is greater than the speed at which the freezing medium flows from the second end to the first end.

[0017] In one embodiment of this disclosure, the width of the first end of the connecting groove is greater than the width of the second end of the connecting groove, or the connecting groove is a Tesla valve groove.

[0018] In one embodiment of this disclosure, a gap is provided between the cap and the body for allowing the refrigerant medium to enter the body.

[0019] In one embodiment of this disclosure, the cryopreservation tube assembly further includes a label attached to the tube cap. The label has a labeling surface facing the side of the tube cap away from the tube body, and the labeling surface is provided with sample information.

[0020] In one embodiment of this disclosure, the marking member is provided with an insertion part, the insertion part is provided with a positioning protrusion, the tube cap is provided with a socket, the insertion part can be inserted into the socket, and the positioning protrusion can abut against the end face of the tube cap facing the tube body.

[0021] In one embodiment of this disclosure, the cap is threadedly connected to the tube body, and the tube body is provided with a first limiting part, which is used to cooperate with the storage container to restrict the tube body from rotating about its own axis.

[0022] In one embodiment of this disclosure, the cap is plugged into and detached from the tube body, and a second limiting portion is provided at the end of the tube body away from the cap. The second limiting portion is used to cooperate with the storage container to restrict the separation of the tube body from the storage container.

[0023] The main beneficial effects of this disclosure are:

[0024] The cryopreservation tube assembly provided in this disclosure, by setting through holes on the bearing surface for positioning the target sample, not only ensures that the target sample is not easily dropped when freezing the target sample and improves the freezing rate, but also improves the thawing rate during thawing, which is beneficial to maintaining the recovery rate and activity of the sample. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the specific embodiments of this disclosure or the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this disclosure. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0026] Figure 1 This is a schematic diagram of the structure of the cryopreservation tube assembly provided in the embodiments of this disclosure;

[0027] Figure 2 This is a schematic diagram of the structure of the cap and support component in the cryopreservation tube assembly provided in the embodiments of this disclosure;

[0028] Figure 3 This is a schematic diagram of the tube body in the cryopreservation tube assembly according to an embodiment of the present disclosure;

[0029] Figure 4 for Figure 3 A magnified view of a section at point I;

[0030] Figure 5 This is a schematic diagram of another structure of the connecting groove in an embodiment of this disclosure;

[0031] Figure 6 A partial structural schematic diagram of the cryopreservation tube assembly provided in an embodiment of this disclosure;

[0032] Figure 7 A schematic diagram of the cryopreservation tube assembly provided in an embodiment of this disclosure from another perspective;

[0033] Figure 8 for Figure 7 Enlarged view of a section at point II;

[0034] Figure 9 This is a front view of the cryopreservation tube assembly provided in an embodiment of this disclosure;

[0035] Figure 10 for Figure 9 A sectional view along line AA;

[0036] Figure 11 for Figure 10 Enlarged view of a section at point III;

[0037] Figure 12 This is a schematic diagram of the structure of the identifier in the cryopreservation tube assembly provided in an embodiment of this disclosure.

[0038] The annotations in the attached figures are explained as follows:

[0039] 11. Pipe cap; 12. Pipe body; 121. Connecting groove; 1211. First end; 1212. Second end; 122. First limiting part; 123. Protrusion; 124. Partition; 13. Bearing member; 131. Rod part; 132. Plate part; 132A. Bearing surface; 1321. Through hole; 14. Counterweight; 141. Discharge hole; 142. Guide part; 15. Identifier; 151. Identifier surface; 152. Insertion part; 1521. Positioning protrusion; 101. Gap. Detailed Implementation

[0040] The technical solutions of this disclosure will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of this disclosure, not all embodiments. Based on the embodiments of this disclosure, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this disclosure.

[0041] In the description of this disclosure, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this disclosure and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this disclosure. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0042] In the description of this disclosure, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this disclosure based on the specific circumstances.

[0043] See Figures 1 to 12 As shown, this embodiment provides a cryopreservation tube assembly, including a cap 11, a tube body 12, and a carrier 13. The cap 11 is detachably connected to the tube body 12, and the carrier 13 is connected to the cap 11. The carrier 13 has a bearing surface 132A for bearing a target sample. The bearing surface 132A is provided with a through hole 1321 for positioning the target sample. The bearing surface 132A can extend into the tube body 12 to place the target sample inside the tube body 12.

[0044] The cryopreservation tube assembly provided in this embodiment has a through-hole 1321 on its bearing surface 132A for positioning the target sample. During use, the target sample is placed at the position of the through-hole 1321, reducing the risk of slippage or loss and making the target sample more stable and reliable during storage and transfer. Simultaneously, both surfaces of the target sample along the depth direction of the through-hole can fully contact the freezing medium, improving the freezing rate. Furthermore, it also improves the thawing rate during thawing, making the target sample easier to detach and helping to maintain the sample's recovery rate and activity.

[0045] It should be understood that before freezing, the sample needs to be dehydrated with an equilibration solution, and before being placed in liquid nitrogen, the target sample needs to be protected with a cryoprotectant. Since the target sample is attached with a small amount of cryoprotectant, it is as if the target sample is wrapped in a droplet. This droplet will have surface tension, so when the target sample is placed in the through hole, it can adhere to the through hole. By setting the through hole, the freezing rate can be ensured to be faster during vitrification freezing.

[0046] For example, the freezing medium can be liquid nitrogen. The target sample can be a biological sample such as a gamete, zygote, or embryo.

[0047] In one embodiment, the through hole 1321 is a round hole with a diameter of 0.2 to 0.7 mm.

[0048] For example, the diameter of the circular hole can be, but is not limited to, 0.2mm, 0.22mm, 0.24mm, 0.26mm, 0.28mm, 0.3mm, 0.35mm, 0.4mm, 0.45mm, 0.5mm, 0.55mm, 0.6mm, 0.65mm, 0.66mm, 0.68mm, 0.69mm, or 0.7mm.

[0049] In some embodiments, the size of the through-hole 1321 may be slightly larger than the size of the target sample.

[0050] In some embodiments, the number of through-holes 1321 is one. Each cryopreservation tube assembly corresponds to one target sample, facilitating one-to-one traceability management of the target sample.

[0051] In one embodiment, see Figure 2 As shown, the bearing member 13 includes a rod portion 131 and a plate portion 132. One end of the rod portion 131 is connected to the cap 11, and the other end of the rod portion 131 is connected to the plate portion 132. The bearing surface 132A is the plate surface of the plate portion 132.

[0052] For example, the rod portion 131 and the plate portion 132 are integrally formed, which facilitates processing and reduces the risk of breakage and detachment.

[0053] It should be noted that the rod 131 and the plate 132 can also be two independent parts, which are then fixedly connected together by means of plugging or gluing.

[0054] For example, a insert tube is provided inside the cap 11, and the rod 131 is inserted into the insert tube to achieve an interference fit. Of course, the rod 131 can also be integrally formed with the cap 11.

[0055] For example, when the support member 13 includes a rod portion 131 and a plate portion 132, the support member 13 can be an incomplete cylinder, which includes a first segment and a second segment. The first segment can be the rod portion 131, which is connected to the cap 11. The second segment is connected to the first segment, and the second segment is provided with a support groove. At least part of the bottom and wall of the support groove forms a support surface 132A. In this case, the support surface 132A can be a curved surface.

[0056] Of course, the second segment can also be a flat plate or a semi-cylinder, in which case the bearing surface can be a plane.

[0057] In one embodiment, the thickness of the plate portion 132 is 0.05 to 0.25 mm. The thickness of the plate portion can be the dimension of the plate portion along the depth direction of the through hole.

[0058] For example, the thickness of the plate portion 132 can be, but is not limited to, 0.05mm, 0.06mm, 0.08mm, 0.1mm, 0.12mm, 0.14mm, 0.16mm, 0.18mm, 0.2mm, 0.22mm, 0.23mm, 0.24mm or 0.25mm.

[0059] Because the plate portion 132 is relatively thin, the freezing and thawing rates of the target sample can be further improved.

[0060] In this embodiment, there is one through hole, and the diameter of the through hole is 0.2 to 0.7 mm, which ensures that the area of ​​the through hole accounts for a small proportion of the plate, which is beneficial to ensuring the mechanical strength of the plate.

[0061] The carrier 13 in this embodiment is smaller in volume than the existing cylindrical structure, which can reduce the space occupied inside the tube 12, thereby allowing more freezing medium to be loaded inside the tube 12, which is more beneficial for the preservation of the target sample.

[0062] In one embodiment, the cross-sectional shape of the plate portion 132 is arc-shaped, V-shaped, wavy, trapezoidal, or concave.

[0063] In some cases, the shape of the bearing surface can be understood as the shape of the cross-section of the plate portion 132.

[0064] In some embodiments, see Figure 2 As shown, the plate portion 132 can be an arc-shaped thin sheet with a thickness of 0.05 to 0.25 mm. The cross-sectional shape of the plate portion 132 is arc-shaped, that is, the bearing groove is an arc-shaped groove.

[0065] In some embodiments, the cross-sectional shape of the plate portion 132 may also be V-shaped, that is, the bearing groove is a V-shaped groove.

[0066] In some embodiments, the cross-sectional shape of the plate portion 132 may also be wavy.

[0067] In some embodiments, the cross-sectional shape of the plate portion 132 can also be U-shaped. Specifically, the plate portion 132 includes a horizontal plate and two vertical plates, which are respectively connected to the two sides of the horizontal plate. Both vertical plates are located on the same side of the horizontal plate. The horizontal plate can be an arc-shaped plate, and the resulting bearing groove is a U-shaped groove. Of course, the horizontal plate can also be a plate with uneven thickness. For example, one surface of the horizontal plate is a plane, and the other surface is a curved surface. For example, the curved surface can be an arc-shaped surface convex towards the plane. In this case, the arc-shaped surface can serve as the bearing surface 132A.

[0068] In some embodiments, plate portion 132 can be a flat plate. In this case, the bearing surface is a plane. Of course, when the cross-sectional shape of plate portion 132 is U-shaped, the transverse plate can be a flat plate, the formed bearing groove can be a straight groove, and the bearing surface can be the surface of the transverse plate, that is, the bearing surface is also a plane.

[0069] In one embodiment, see Figure 3 and Figure 4 As shown, the inner wall of the tube body 12 is provided with a connecting groove 121. Along the extending direction of the connecting groove 121, the connecting groove 121 has a first end 1211 and a second end 1212. The first end 1211 of the connecting groove 121 penetrates the end face of the tube body 12 facing the tube cap 11, and the second end 1212 of the connecting groove 121 is located inside the tube body 12. The connecting groove 121 is configured such that the speed at which the refrigerant flows from the first end 1211 to the second end 1212 is greater than the speed at which the refrigerant flows from the second end 1212 to the first end 1211.

[0070] By setting up the connecting groove 121, the inflow and outflow rates of liquid nitrogen can be controlled. By increasing the rate at which liquid nitrogen enters the tube 12, instantaneous ultra-low temperature freezing of the target sample can be achieved during cryopreservation clinical operations. At the same time, by slowing down the rate at which liquid nitrogen flows out of the tube 12, the liquid nitrogen in the tube 12 is ensured to overflow and evaporate as slowly as possible during thawing clinical operations, thereby meeting the needs of clinical operations.

[0071] In one embodiment, the width of the first end 1211 of the connecting groove 121 is greater than the width of the second end 1212 of the connecting groove 121.

[0072] For example, see Figure 4 As shown, the connecting channel 121 can be a V-shaped channel. When liquid nitrogen flows from the wider first end 1211 to the narrower second end 1212 of the connecting channel 121, the flow velocity increases, allowing the liquid nitrogen to quickly flow into the tube 12 for instantaneous cryogenic freezing of the target sample. When liquid nitrogen needs to overflow or evaporate from the tube 12, it needs to pass through the narrower second end 1212 of the connecting channel 121 before flowing back to the wider first end 1211, thus slowing down the flow velocity.

[0073] Of course, the connecting groove 121 can also be a U-shaped groove.

[0074] In some embodiments, see Figure 5 As shown, the connecting groove 121 can also be a Tesla valve groove. When liquid nitrogen flows from the first end 1211 to the second end 1212 of the Tesla groove, the flow rate increases, and when liquid nitrogen flows from the second end 1212 to the first end 1211 of the Tesla groove, the flow rate decreases.

[0075] In one embodiment, see Figure 6As shown, a gap 101 is provided between the cap 11 and the tube body 12 for the refrigerant to enter the tube body 12. Exemplarily, a gap 101 is provided between the end faces of the cap 11 and the tube body 12 that are close to each other for the refrigerant to enter the tube body 12. Since the first end 1211 of the connecting groove 121 penetrates the end face of the tube body 12 facing the cap 11, the connecting groove 121 communicates with the gap 101. Liquid nitrogen can flow through this gap 101 to the connecting groove 121 and enter the tube body 12. Simultaneously, since the gap 101 between the end faces of the cap 11 and the tube body 12 in this embodiment is small, the small gap 101, in conjunction with the connecting groove 121, can achieve rapid entry of liquid nitrogen and slow down the overflow and evaporation rate of liquid nitrogen. At the same time, it can also effectively filter and block fine impurities in the liquid nitrogen.

[0076] In one embodiment, the cap 11 is threadedly connected to the body 12, see [reference]. Figure 1 and Figure 3 As shown, the tube body 12 is provided with a first limiting part 122, which is used to cooperate with the storage container (not shown in the figure) to restrict the tube body 12 from rotating about its own axis.

[0077] For example, the cap 11 is provided with an external thread, and the body 12 is provided with an internal thread, which mates with the external thread. In this embodiment, the connecting groove 121 penetrates the internal thread, that is, at the location where the connecting groove 121 is provided, the internal thread is disconnected.

[0078] In some embodiments, a first limiting engagement part (not shown in the figure) is provided on the upper surface of the bottom plate of the storage container. When the cap 11 is connected or separated from the tube body 12, the limiting part on the tube body 12 cooperates with the limiting engagement part on the storage container to restrict the tube body 12 from rotating around its own axis, thereby allowing the cap 11 to rotate relative to the tube body 12, so as to realize the connection or separation of the cap 11 and the tube body 12.

[0079] For example, the first limiting part 122 can be a first anti-rotation rib, and the first limiting mating part can be a second anti-rotation rib. There can be multiple first and second anti-rotation ribs. Multiple first anti-rotation ribs are spaced apart along the circumferential outer surface of the tube body 12, and multiple second anti-rotation ribs are disposed on the bottom plate of the storage container, each mating with a corresponding first anti-rotation rib. When the tube cap 11 is screwed onto the tube body 12, the second anti-rotation ribs can abut against the first anti-rotation ribs to restrict the tube body 12 from rotating around its own axis, thereby facilitating the tightening of the tube cap 11 onto the tube body 12.

[0080] For example, the outline shape of the first anti-rotation rib can be a rectangle or a triangle, such as an obtuse triangle.

[0081] It should be noted that the structural forms of the first limiting part and the first limiting mating part are not limited to the one mentioned above. As long as they can achieve the function of restricting the tube body from rotating around its own axis so that the tube cap 11 can be smoothly connected or separated from the tube body 12, it is acceptable. For example, one of the first limiting part 122 and the first limiting mating part can be a slot, and the extension direction of the slot can be parallel to the axial direction of the tube body 12. The other can be a protrusion. During the process of placing the tube body 12 into the storage container, the protrusion can be inserted into the slot, and the groove wall of the slot abuts against the surface of the protrusion.

[0082] In other embodiments, the cap 11 and the tube body 12 can also be connected by a plug-in method. In this case, a second limiting part is provided at the end of the tube body 12 away from the cap 11. The second limiting part is used to cooperate with the storage container to prevent the tube body 12 from separating from the storage container.

[0083] For example, the second limiting part can be a first magnetic element, and the second limiting mating part can be a second magnetic element. The first magnetic element and the second magnetic element attract each other to fix the tube body 12. For example, the first magnetic element and the second magnetic element can be two magnets that attract each other.

[0084] In one embodiment, the cryopreservation tube assembly further includes a counterweight 14, which is installed at the end of the tube body 12 away from the cap 11. By setting the counterweight 14, the cryopreservation tube assembly is ensured to sink into the freezing medium and not float on the surface of the freezing medium, thus ensuring the freezing effect.

[0085] In one embodiment, see Figure 10 and Figure 11 As shown, the tube body 12 is provided with a partition 124, which is used to divide the internal space of the tube body 12 into a first space and a second space. The first space is used to accommodate the target sample, and the counterweight 14 is installed in the second space.

[0086] In one embodiment, see Figure 11 As shown, the counterweight 14 is provided with a discharge hole 141, which extends through the counterweight 14 along the axial direction of the tube 12. Liquid nitrogen can flow through the gap 101 to the connecting groove 121 and enter the first space of the tube 12. Since the tube 12 is immersed in liquid nitrogen, liquid nitrogen will also be present in the second space. The residual liquid nitrogen in the second space can be discharged through the discharge hole 141 of the counterweight 14, preventing the tube 12 from cracking due to energy accumulation during the vaporization of residual liquid nitrogen.

[0087] For example, the counterweight 14 can be a hollow cylinder. The counterweight 14 can be made of stainless steel, which has a density greater than that of liquid nitrogen, so that the cryopreservation tube assembly can be immersed in liquid nitrogen, ensuring stable temperature inside the tube 12 and good cryopreservation effect.

[0088] It should be noted that in the scheme of setting the first magnetic element at the end of the tube body 12 away from the tube cap 11, the first magnetic element can be set on the side of the counterweight block 14 away from the tube cap 11.

[0089] In one embodiment, the surface of the counterweight 14 facing the partition 124 is provided with a flow guide 142, which is used to guide at least a portion of the refrigerant in the second space to the discharge port 141.

[0090] For example, the flow guide 142 can be a recess provided on the surface of the counterweight 14 facing the partition 124. See also Figure 10 and Figure 11 As shown, the surface of the counterweight 14 facing the partition 124 can be the circumferential side of a frustum or a spherical cap surface protruding away from the partition 124. The refrigerant remaining in the second space can flow into the discharge hole 141 under the guidance of the circumferential side of the frustum or the spherical cap surface, and then be discharged from the discharge hole 141.

[0091] It should be understood that when there is a gap between the circumferential sidewall of the counterweight 14 and the inner wall of the tube 12, the refrigerant can also be discharged from the gap between them.

[0092] In one embodiment, at least one of the counterweight 14 and the tube 12 is provided with a protrusion 123, which is used to restrict the movement of the counterweight 14 along the axial direction of the tube 12.

[0093] In some embodiments, a protrusion 123 is disposed on the inner wall of the end of the tube body 12 away from the cap 11, and the protrusion 123 abuts against the surface of the counterweight 14 away from the cap 11. See also Figure 8 As shown, the protrusion 123 is integrally formed with the tube body 12, and there can be multiple protrusions 123. The inner diameter of the end of the tube body 12 away from the cap 11 is slightly larger than the outer diameter of the counterweight 14. When the counterweight 14 is installed into the tube body 12, the protrusion 123 will press against the surface of the counterweight 14 away from the cap 11 to achieve a snap-fit ​​assembly and prevent the counterweight 14 from falling off.

[0094] For example, the protrusion 123 can be a crown-shaped protrusion structure. Of course, the protrusion 123 can also be a strip-shaped rib.

[0095] In other embodiments, the counterweight 14 is interference-fitted with the tube body 12 by means of a protrusion.

[0096] For example, the protrusion can be provided on the circumferential sidewall of the counterweight 14, or on the inner wall of the end of the tube body 12 away from the cap 11, i.e., the cavity wall of the second space. Of course, both the circumferential sidewall of the counterweight 14 and the cavity wall of the second space of the tube body 12 can be provided with protrusions. During assembly, the protrusions on the circumferential sidewall of the counterweight 14 and the protrusions on the cavity wall of the second space can be staggered to enhance the limiting effect.

[0097] It should be noted that the counterweight 14 can also be fixedly installed on the tube body 12 by means of a snap-fit ​​or other structure.

[0098] In one embodiment, see Figure 12 As shown, the cryopreservation tube assembly also includes a label 15, which is installed on the tube cap 11. The label 15 has a labeling surface 151 facing the side of the tube cap 11 away from the tube body 12, and the labeling surface 151 is provided with sample information.

[0099] For example, the sample information can be a QR code, which can be laser-etched onto the marking surface 151. The QR code can enable automatic and quick storage and identification of sample information, and realize one-to-one traceability management of the sample.

[0100] In some embodiments, see Figure 12 As shown, the label 15 is provided with an insertion part 152, the insertion part 152 is provided with a positioning protrusion 1521, the tube cap 11 is provided with a socket, the insertion part 152 can be inserted into the socket, and the positioning protrusion 1521 can abut against the end face of the tube cap 11 facing the tube body 12.

[0101] For example, the insertion part 152 can be integrally formed with the label 15. The insertion part 152 is located on the side of the label 15 away from the label surface 151. When the insertion part 152 is inserted into the socket, the positioning protrusion 1521 abuts against the end face of the tube cap 11 facing the tube body 12. At this time, the label 15, the tube cap 11, and the carrier 13 are combined to form an integral whole, which can be connected or separated from the tube body 12 together.

[0102] See Figure 2 As shown, the insertion part 152 can indicate the orientation of the bearing surface 132A. During operation, the insertion part 152 is easily visible to the operator. By following the extension direction of the insertion part 152, the bearing surface 132A can be determined, thereby placing the target sample in the through hole 1321 of the bearing surface 132A. This effectively prevents the operator from mistakenly placing the target sample on the side of the carrier 13 away from the bearing surface 132A.

[0103] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this disclosure, and are not intended to limit them. Although this disclosure has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this disclosure.

Claims

1. A cryopreservation tube assembly, characterized in that, The device includes a cap, a body, and a carrier. The cap is detachably connected to the body, and the carrier is connected to the cap. The carrier has a bearing surface for bearing a target sample, and the bearing surface is provided with a through hole for positioning the target sample. The bearing surface can extend into the body of the body to place the target sample inside the body.

2. The cryopreservation tube assembly according to claim 1, characterized in that, The number of through holes is one; and / or, the through hole is a round hole with a diameter of 0.2 to 0.7 mm.

3. The cryopreservation tube assembly according to claim 1, characterized in that, The bearing member includes a rod and a plate. One end of the rod is connected to the cap, and the other end of the rod is connected to the plate. The bearing surface is the surface of the plate.

4. The cryopreservation tube assembly according to claim 3, characterized in that, The thickness of the plate is 0.05 to 0.25 mm.

5. The cryopreservation tube assembly according to claim 3, characterized in that, The plate is a flat plate, or the cross-sectional shape of the plate is arc-shaped, V-shaped, wavy, trapezoidal, or concave.

6. The cryopreservation tube assembly according to claim 1, characterized in that, The cryopreservation tube assembly also includes a counterweight, which is installed at the end of the tube body away from the tube cap.

7. The cryopreservation tube assembly according to claim 6, characterized in that, The tube is provided with a partition, which is used to divide the internal space of the tube into a first space and a second space. The first space is used to accommodate the target sample, and the counterweight is installed in the second space.

8. The cryopreservation tube assembly according to claim 7, characterized in that, The counterweight is provided with a discharge hole, which extends through the counterweight along the axial direction of the tube.

9. The cryopreservation tube assembly according to claim 8, characterized in that, The counterweight has a flow guide on its surface facing the partition, which is used to guide at least a portion of the refrigerant in the second space to the discharge hole.

10. The cryopreservation tube assembly according to claim 6, characterized in that, At least one of the counterweight and the tube is provided with a protrusion, which is used to restrict the movement of the counterweight along the axial direction of the tube.

11. The cryopreservation tube assembly according to claim 10, characterized in that, The protrusion is disposed on the inner wall of the end of the tube away from the cap, and the protrusion abuts against the surface of the counterweight away from the cap.

12. The cryopreservation tube assembly according to claim 10, characterized in that, The counterweight is press-fitted to the tube body via the protrusion; the protrusion is located on the circumferential sidewall of the counterweight, and / or the protrusion is located on the inner wall of the tube body at the end away from the cap.

13. The cryopreservation tube assembly according to any one of claims 1 to 12, characterized in that, The inner wall of the tube is provided with a connecting groove. Along the extending direction of the connecting groove, the connecting groove has a first end and a second end. The first end of the connecting groove passes through the end face of the tube facing the tube cap, and the second end of the connecting groove is located inside the tube. The connecting groove is configured such that the speed at which the freezing medium flows from the first end to the second end is greater than the speed at which the freezing medium flows from the second end to the first end.

14. The cryopreservation tube assembly according to claim 13, characterized in that, The width of the first end of the connecting groove is greater than the width of the second end of the connecting groove, or the connecting groove is a Tesla valve groove.

15. The cryopreservation tube assembly according to any one of claims 1 to 12, characterized in that, There is a gap between the cap and the tube body for allowing the refrigerant to enter the tube body.

16. The cryopreservation tube assembly according to any one of claims 1 to 12, characterized in that, The cryopreservation tube assembly also includes a label, which is installed on the tube cap. The label has a labeling surface facing the side of the tube cap away from the tube body, and the labeling surface is provided with sample information.

17. The cryopreservation tube assembly according to claim 16, characterized in that, The marking component is provided with an insertion part, the insertion part is provided with a positioning protrusion, the tube cap is provided with a socket, the insertion part can be inserted into the socket, and the positioning protrusion can abut against the end face of the tube cap facing the tube body.

18. The cryopreservation tube assembly according to any one of claims 1 to 12, characterized in that, The cap is threadedly connected to the tube body, and the tube body is provided with a first limiting part, which is used to cooperate with the storage container to restrict the tube body from rotating around its own axis.

19. The cryopreservation tube assembly according to any one of claims 1 to 12, characterized in that, The cap is plugged into and detached from the tube body. A second limiting part is provided at the end of the tube body away from the cap. The second limiting part is used to cooperate with the storage container to prevent the tube body from separating from the storage container.

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