A real-time fluorescence quantitative PCR device

Abstract

This invention discloses a real-time quantitative PCR device, relating to the field of quantitative PCR equipment technology. It includes a device platform, with a detection chamber fixedly installed on one side of the top of the platform. A sample plate is slidably disposed inside the detection chamber. The sample plate has multiple hanging holes arranged in a rectangular array, and reagent tubes are installed inside the hanging holes. An assembly is also provided on the sample plate. The assembly includes two connecting brackets movably mounted on the top of the sample plate, with threaded rods fixedly installed inside the connecting brackets. By setting up this assembly, during loading and unloading, rotating the threaded rods can drive a translation rod to connect with the reagent tubes, and lifting the connecting brackets can simultaneously lift or lower all reagent tubes, thereby achieving batch sample extraction and loading. This significantly shortens the time required to open the chamber door, reduces the risk of internal contamination, and improves detection efficiency.

Description

Technical Field

[0001] This utility model relates to the field of fluorescence quantitative PCR equipment technology, and in particular to a real-time fluorescence quantitative PCR device. Background Technology

[0002] The existing publication number (CN217948119U) discloses a real-time quantitative PCR device. This device features a movable reagent plate that automatically moves with the opening and closing of the compartment door. This ensures the reagent plate is automatically removed when the door is open, eliminating the need to reach inside the device to change samples and reducing the risk of internal contamination. However, in actual use, especially during sample handling, sample tubes need to be removed or placed into the reagent plate one by one. This process is time-consuming, resulting in the compartment door remaining open for an extended period, which increases the risk of internal contamination and affects the test results of subsequent samples. Utility Model Content

[0003] To address the shortcomings of existing technologies, this invention provides a real-time fluorescence quantitative PCR device, which solves the technical problems mentioned in the background section.

[0004] To solve the above technical problems, the present invention provides the following technical solution: a real-time fluorescence quantitative PCR device, including a device platform, a detection chamber fixedly installed on one side of the top of the device platform, a sample plate slidably arranged inside the detection chamber, a plurality of hanging holes arranged in a rectangular array on the sample plate, a reagent tube installed inside the hanging holes, and an assembly provided on the sample plate;

[0005] The assembly includes two connecting frames movably mounted on the top of the sample plate. Threaded rods are fixedly installed inside the connecting frames. Multiple pairs of translation rods are connected to the common threads of the two threaded rods. Each pair of translation rods corresponds to multiple rows of reagent tubes. Mounting holes are provided on both sides of each reagent tube. Multiple mounting rods that match the mounting holes are fixedly installed on the side of each translation rod closest to the reagent tube.

[0006] Furthermore, the threaded rod is provided with multiple pairs of threads with opposite directions on its exterior, and multiple pairs of translation rods are respectively provided on the multiple pairs of threads.

[0007] Furthermore, a guide block is fixedly connected to the front end of the connecting frame, and a guide seat for inserting the guide block is fixedly provided on the front side of the sample plate.

[0008] Furthermore, a handle is fixedly installed at the center of the top of the connecting frame.

[0009] Furthermore, tracks are fixedly installed on both sides of the inner wall of the detection chamber, and multiple pairs of rollers that are used in conjunction with the tracks are installed on the bottom of the sample plate.

[0010] Furthermore, at least two sets of placement racks are placed on top of the equipment platform.

[0011] By employing the above technical solution, this utility model provides a real-time fluorescence quantitative PCR device, which has at least the following beneficial effects:

[0012] 1. By setting up an assembly, this utility model allows the rotating threaded rod to drive the translation rod to connect with the reagent tube during loading and unloading. The lifting connecting frame can lift or lower all reagent tubes simultaneously, thereby realizing batch sample extraction and loading, significantly shortening the time of opening the chamber door, reducing the risk of internal contamination of the equipment, and improving detection efficiency.

[0013] 2. By setting guide blocks and guide seats, this utility model facilitates the alignment of the mounting rod with the mounting hole, ensuring the smooth assembly of multiple reagent tubes for subsequent loading and unloading operations. Attached Figure Description

[0014] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings:

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the detection box structure of this utility model;

[0017] Figure 3 This is a schematic diagram of the sample plate structure of this utility model;

[0018] Figure 4 This is a schematic diagram of the guide seat structure of this utility model;

[0019] Figure 5 This is a schematic diagram of the assembly structure of this utility model;

[0020] Figure 6 This is a schematic diagram of the reagent tube structure of this utility model.

[0021] In the diagram: 1. Equipment platform; 2. Testing chamber; 3. Sample plate; 301. Hanging hole; 4. Reagent tube; 401. Mounting hole; 5. Assembly; 51. Connecting frame; 52. Threaded rod; 53. Translation rod; 54. Mounting rod; 55. Guide block; 56. Guide seat; 57. Handle; 6. Track; 7. Roller; 8. Placement rack. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] In actual use, especially during sample handling, existing equipment requires removing or placing sample reagent tubes one by one from or into the reagent plate. This process takes a long time, resulting in the door being open for an extended period. This also increases the risk of contamination inside the equipment, affecting the test results of the next set of samples.

[0024] Example 1

[0025] To address the deficiencies in the sample handling process of the aforementioned equipment, please refer to [link / reference needed]. Figures 1-6The present invention provides a real-time fluorescence quantitative PCR device that enables simultaneous loading and unloading of multiple reagent tubes, shortens the opening time of the chamber door, and reduces the probability of contamination of the equipment. The device is based on a platform 1. A detection chamber 2 is fixedly installed on one side of the top of the platform 1. A sample plate 3 is slidably arranged inside the detection chamber 2. The sample plate 3 has multiple hanging holes 301 arranged in a rectangular array. Reagent tubes 4 are installed inside the hanging holes 301, and samples are added to the reagent tubes 4. An assembly 5 is provided on the sample plate 3 to assemble all the reagent tubes 4 on the sample plate 3 together, which facilitates the synchronous loading and unloading of all the reagent tubes 4. The assembly 5 includes two connecting brackets 51 movably installed on the top of the sample plate 3. Threaded rods 52 are fixedly installed inside the connecting brackets 51. The two threaded rods 52 are connected to multiple pairs of translation rods 53 by a common thread. The threaded rods 52 have multiple pairs of threads with opposite directions on their exterior. The multiple pairs of translation rods 53 are respectively arranged on the multiple pairs of threads. The translation rods 53 are slidably connected to the inner wall of the connecting brackets 51. Rotating the threaded rods 52 can drive the translation rods 53 to slide. The multiple pairs of translation rods 53 are respectively opposite to multiple rows of reagent tubes 4. The reagent tube 4 is positioned between two translation rods 53 of the same pair. Mounting holes 401 are provided on both sides of the reagent tube 4. Multiple mounting rods 54, which are compatible with the mounting holes 401, are fixedly installed on the side of the translation rod 53 closest to the reagent tube 4. A knob is fixedly connected to the front end of the threaded rod 52 through the connecting frame 51. During unloading, rotating the knob facilitates the rotation of the threaded rod 52. The rotation of the threaded rod 52 causes the two translation rods 53 of the same pair to move closer together. The translation rod 53 slides, causing the mounting rod 54 to insert into the mounting hole 401, thus connecting the translation rod 53 to the reagent tube 4. This allows the connecting frame 51 to be lifted. Due to the action of the mounting rod 54, all reagent tubes 4 can be lifted upwards until they are pulled out of the hanging hole 301 in the reagent tube 4. This allows all reagent tubes 4 to be removed from the sample plate 3, achieving synchronous unloading of all reagent tubes 4. The same principle applies to loading; there is no need to individually remove or place the reagent tubes 4, greatly saving loading and unloading time and effectively reducing the probability of contamination inside the detection chamber 2.

[0026] To facilitate the removal of the reagent tube 4 from the hanging hole 301, a handle 57 is fixedly installed at the center of the top of the connecting frame 51, which facilitates the upward lifting of the connecting frame 51.

[0027] When the connecting frame 51 is lifted upwards, the sample plate 3 will be inconvenient to operate inside the detection box 2. Therefore, rails 6 are fixedly installed on both sides of the inner wall of the detection box 2, and multiple pairs of rollers 7 that are used in conjunction with the rails 6 are installed on the bottom of the sample plate 3. This allows the sample plate 3 to be pulled out of the detection box 2 for loading and unloading operations.

[0028] Since the reagent tubes 4 taken out from the sample plate 3 need to be placed, at least two sets of placement racks 8 are placed on the top of the equipment table 1. One set of placement racks 8 is used to place the reagent tubes 4 that have been tested, and the other set of placement racks 8 is used to place the reagent tubes 4 to be tested. This makes it convenient to combine the reagent tubes 4 to be tested together and put them into the testing chamber 2, saving loading time.

[0029] Example 2

[0030] When loading and unloading reagent tube 4, ensure that mounting rod 54 is aligned with mounting hole 401. Please refer to [reference needed]. Figure 4 As shown, a guide block 55 is fixedly connected to the front end of the connecting frame 51, and a guide seat 56 for the guide block 55 to be inserted is fixedly provided on the front side of the sample plate 3. The guide block 55 and the guide seat 56 can position the connecting frame 51. When the connecting frame 51 is snapped onto the sample plate 3, it is only necessary to align the guide block 55 and the guide seat 56. This ensures that the mounting rod 54 is aligned with the mounting hole 401, and that the mounting rod 54 can be smoothly inserted into the mounting hole 401 to connect multiple reagent tubes 4.

[0031] It should be noted that, in this document, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A real-time fluorescent quantitative PCR apparatus comprising an apparatus table (1), characterized in that: A detection box (2) is fixedly installed on one side of the top of the equipment platform (1). A sample plate (3) is slidably arranged inside the detection box (2). Multiple hanging holes (301) arranged in a rectangular array are opened on the sample plate (3). A reagent tube (4) is installed inside the hanging hole (301). An assembly (5) is provided on the sample plate (3). The assembly (5) includes two connecting brackets (51) movably mounted on the top of the sample plate (3). Threaded rods (52) are fixedly installed inside the connecting brackets (51). The two threaded rods (52) are connected by a common thread to multiple pairs of translation rods (53). The multiple pairs of translation rods (53) correspond to multiple rows of reagent tubes (4). Mounting holes (401) are provided on both sides of each reagent tube (4). Multiple mounting rods (54) that are adapted to the mounting holes (401) are fixedly provided on the side of the translation rods (53) near the reagent tubes (4).

2. The real-time fluorescence quantitative PCR device according to claim 1, characterized in that: The threaded rod (52) has multiple pairs of threads with opposite directions on its exterior, and multiple pairs of translation rods (53) are respectively arranged on the multiple pairs of threads.

3. The real-time fluorescence quantitative PCR device according to claim 1, characterized in that: The front end of the connecting frame (51) is fixedly connected to a guide block (55), and the front side of the sample plate (3) is fixedly provided with a guide seat (56) for the guide block (55) to be inserted.

4. The real-time fluorescence quantitative PCR device according to claim 1, characterized in that: A handle (57) is fixedly installed at the center of the top of the connecting frame (51).

5. The real-time fluorescence quantitative PCR device according to claim 1, characterized in that: The inner walls of the detection box (2) are fixedly installed with rails (6) on both sides, and the bottom of the sample plate (3) is equipped with multiple pairs of rollers (7) that are used in conjunction with the rails (6).

6. The real-time fluorescence quantitative PCR device according to claim 1, characterized in that: At least two sets of placement racks (8) are placed on the top of the equipment table (1).

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