Biological reagent detection equipment

Abstract

The utility model belongs to the technical field of biological reagent detection, and particularly relates to biological reagent detection equipment. The utility model provides biological reagent detection equipment and aims to solve the problem of low temperature control efficiency of biological reagent detection equipment in the prior art. Biological reagent detection equipment comprises a reagent loading assembly used for loading a thin film chip with a built-in reagent; the temperature control assembly is used for heating or cooling the reagent; the light analysis component is used for analyzing light emitted by the reagent; and the optical assembly is used for guiding the light emitted by the reagent to the light analysis unit. The reagent loading assembly is used for loading the thin film chip with the reagent inside, and the thin film chip is thin and high in heat exchange efficiency, so that the biological reagent detection equipment is high in temperature control efficiency, the thin film chip is low in heat transfer loss, and the temperature increasing and decreasing speed is high.

Description

Technical Field

[0001] This utility model belongs to the field of biological reagent detection technology, and specifically relates to a biological reagent detection device. Background Technology

[0002] Polymerase chain reaction (PCR) works by first heating the double-stranded DNA to unwind, yielding template DNA. Under annealing conditions, primers hybridize with the template DNA. Primers are then extended using Taq DNA polymerase, Mg2+, and a suitable pH buffer. This cycle of unwinding, hybridization, extension, and unwinding is repeated 25-40 times, resulting in an exponential increase in the nucleic acid copy number in the sample. Temperature control is required for each of the unwinding, hybridization, and extension stages; each PCR cycle is a temperature cycle. Therefore, nucleic acid amplification microfluidic chips must be capable of rapid heating and cooling to complete the temperature cycling process of PCR.

[0003] To meet the clinical demand for high temperature control efficiency in nucleic acid amplification microfluidic chips, it is necessary to minimize chip thickness to facilitate temperature control by external heating devices. However, current chip manufacturing processes primarily utilize injection molding, which can only process plate-shaped chips with a certain thickness. When the chip thickness is reduced to a certain level, the complex and delicate structures such as channels and reaction cells within the chip become unsuitable for precision machining by injection molding. Therefore, existing biological reagent detection equipment suffers from low temperature control efficiency. Utility Model Content

[0004] This invention provides a biological reagent detection device, which aims to solve the problem of low temperature control efficiency in existing biological reagent detection devices.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows:

[0006] A biological reagent detection device includes a reagent loading assembly for loading a thin-film chip containing built-in reagents;

[0007] Temperature control components for heating or cooling reagents;

[0008] A light analysis component used to analyze the light emitted by reagents;

[0009] An optical component for guiding the light emitted by the reagent to the light analysis unit.

[0010] A further improved solution: The temperature control component includes a semiconductor refrigeration chip.

[0011] Based on the above technical solution: the temperature control component includes a semiconductor refrigeration chip. By passing currents of different polarities through the semiconductor refrigeration chip, the semiconductor refrigeration chip can be heated or cooled, which reduces the volume of the temperature control component and thus reduces the volume of the biological reagent detection equipment.

[0012] A further improved solution: The temperature control component includes a first temperature zone with a first temperature and a second temperature zone with a second temperature.

[0013] Based on the above technical solution: the temperature control component includes a first temperature zone and a second temperature zone. By bringing the reagent into contact with the first temperature zone or the second temperature zone, the reagent can be subjected to different temperatures, thereby improving the temperature control efficiency of the temperature control component.

[0014] A further improved solution: the temperature control component is slidably connected relative to the loading component, and the reagent alternately exchanges heat with the first temperature zone and the second temperature zone.

[0015] Based on the above technical solution: the temperature control component is slidably connected to the loading component, the reagent is in contact with the first temperature zone and the second temperature zone respectively, and the reagent and the temperature control component have a larger heat exchange area.

[0016] A further improved solution: Both the first temperature zone and the second temperature zone have heat exchange chambers, and the reagents built into the loading assembly are located in the heat exchange chambers.

[0017] Based on the above technical solution: both the first and second temperature zones have heat exchange chambers, and the reagents are located inside the heat exchange chambers. The reagents and temperature control components have a larger heat exchange area, which further improves the detection efficiency of the reagents.

[0018] A further improved solution: The optical component includes a light receiving unit that receives the light output from the reagent.

[0019] Based on the above technical solution: the light receiving unit is used to receive light, which is beneficial for the analysis of optical analysis components.

[0020] A further improved solution: The optical component includes a light output unit that inputs excitation light into the reagent.

[0021] Based on the above technical solution, the optical component also includes a light output unit, which is used to input excitation light into the reagent.

[0022] A further improved solution: The light output unit includes a light source, and there are at least two light sources.

[0023] Based on the above technical solution: the light output unit includes a light source, and there are at least two light sources. Two light sources can provide stronger light, which is beneficial for the light analysis unit to perform optical analysis.

[0024] A further improved solution: each of the light sources emits light of an independent wavelength.

[0025] Based on the above technical solution: each light source emits light of an independent wavelength, and the light output by the reagent can be analyzed by the light analysis component without filtering.

[0026] A further improvement: The light analysis component includes a light analysis chip.

[0027] Based on the above technical solution: the light analysis component includes a light analysis chip, which is small in size, further simplifying the size of the biological reagent detection equipment.

[0028] The beneficial effects of this utility model are as follows:

[0029] A biological reagent detection device includes a reagent loading assembly for loading a thin-film chip containing a built-in reagent; a temperature control assembly for heating or cooling the reagent; a light analysis assembly for analyzing the light emitted by the reagent; and an optical assembly for guiding the light emitted by the reagent to the light analysis unit. The reagent loading assembly loads the thin-film chip containing the reagent. The thin-film chip is thin and has high heat exchange efficiency, resulting in high temperature control efficiency for the biological reagent detection device. The thin-film chip also exhibits low heat transfer loss and rapid heating and cooling rates. Attached Figure Description

[0030] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For users of ordinary skills in the art, other related drawings can be obtained from these drawings without creative effort.

[0031] Figure 1 This is a schematic diagram of a biological reagent detection device according to the present invention, taken in the first direction.

[0032] Figure 2 This is a schematic diagram of the second direction of a biological reagent detection device according to the present invention.

[0033] Figure 3 This is a schematic diagram of the reagent loading component in the first direction of a biological reagent detection device according to this utility model.

[0034] Figure 4 This is a schematic diagram of the reagent loading component in the second direction of a biological reagent detection device according to the present invention.

[0035] Explanation of the labels in the diagram:

[0036] 1-Reagent loading assembly; 2-Temperature control assembly; 3-Light analysis assembly; 4-Optical assembly; 5-Thin film chip. Detailed Implementation

[0037] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. It should be understood that the specific embodiments described herein are merely for explaining the present utility model and are not intended to limit the present utility model. All other embodiments obtained by users of the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0038] refer to Figures 1 to 4 This embodiment provides a biological reagent detection device, including a reagent loading assembly 1 for loading a thin film chip 5 containing reagents;

[0039] Temperature control component 2 is used to heat or cool the reagent;

[0040] Light analysis component 3 is used to analyze the light emitted by the reagents;

[0041] Optical component 4 is used to guide the light emitted by the reagent to the light analysis unit.

[0042] The thin-film chip 5 can be made of thin film. For example, the edges of two thin films can be heat-sealed to form the thin-film chip 5.

[0043] Alternatively, the thin film chip 5 can also be formed by bending a thin film. After bending the thin film, it is heat-sealed at the edge to form the thin film chip 5. The bent part of the thin film chip 5 can input and output light.

[0044] Wherein: the temperature control component 2 includes a semiconductor refrigeration chip.

[0045] Wherein: This scheme is a scheme parallel to the semiconductor cooling chip, and the temperature control component 2 includes a first temperature zone with a first temperature and a second temperature zone with a second temperature.

[0046] refer to Figures 1 to 4 Specifically: the temperature control component 2 is slidably connected relative to the loading component, and the reagent alternately exchanges heat with the first temperature zone and the second temperature zone.

[0047] Both the first temperature zone and the second temperature zone have heat exchange chambers, and the reagents built into the loading assembly are located in the heat exchange chambers.

[0048] Wherein: the optical component 4 includes a light receiving unit for receiving the light output from the reagent.

[0049] The optical component 4 includes a light output unit that inputs excitation light into the reagent. When the reagent has a self-luminous function, the optical component 4 may not include a light output unit.

[0050] Specifically: the light output unit includes a light source, and there are at least two light sources.

[0051] Each of the light sources emits light at an independent wavelength.

[0052] The light analysis component 3 includes a light analysis chip. The light analysis chip is used to fix the light emitted by the analytical reagent. The light emitted by the reagent may contain light of different wavelengths, and the light analysis chip may need to perform different analyses on light of different wavelengths. Therefore, when multiple light sources emitting light of different wavelengths are set, the light analysis chip can directly analyze the light output by the reagent.

[0053] When a biological reagent detection device is integrated into a single product, the reagent loading component 1, temperature control component 2, light analysis component 3, and optical component 4 can be installed in the same housing using any method, such as fixing with screws.

[0054] When a biological reagent detection device is integrated into a detection platform, the reagent loading component 1, temperature control component 2, light analysis component 3, and optical component 4 can be installed in different housings, for example, by fixing them with screws.

[0055] The working principle of this embodiment:

[0056] A sample is added to the thin film chip 5. The sample can be nasopharyngeal swab extract, etc. The thin film chip 5 includes at least a detection chamber for containing a reagent containing the sample. The reagent containing the sample can be added to the detection chamber in any way. For example, the reagent containing the sample can be squeezed into the detection chamber.

[0057] The thin-film chip 5 containing the reagent is mounted on the reagent loading assembly;

[0058] Turn on the temperature control component 2 to control the temperature of the reagent in the detection chamber. For example, the first temperature zone is 95°C and the second temperature zone is 60°C. Control the temperature control component 2 to move relative to the reagent loading component 1 so that the reagent can exchange heat in the first temperature zone and the second temperature zone respectively, thereby realizing the temperature circulation of the reagent.

[0059] After the reagent completes a certain number of temperature cycles, optical component 4 is turned on to input excitation light into the reagent. The number of temperature cycles is usually 30 or 60 times.

[0060] After the excitation light enters the reagent, it is reflected by the reagent. The reflected light enters the light receiving unit, and the light entering the light receiving unit is finally analyzed by the optical analysis chip to complete the biological reagent detection.

[0061] This utility model is not limited to the above-mentioned optional embodiments. Under the premise of non-contradiction, the various solutions can be combined arbitrarily. Anyone can derive other forms of products under the guidance of this utility model. However, no matter what changes are made in their shape or structure, all technical solutions that fall within the scope of the claims of this utility model are within the protection scope of this utility model.

Claims

1. A biological reagent detection device, characterized in that: Includes a reagent loading assembly for loading a thin-film chip containing built-in reagents; Temperature control components for heating or cooling reagents; A light analysis component used to analyze the light emitted by reagents; An optical component for guiding the light emitted by the reagent to the light analysis unit.

2. The biological reagent detection device according to claim 1, characterized in that: The temperature control component includes a semiconductor refrigeration chip.

3. The biological reagent detection device according to claim 1, characterized in that: The temperature control component includes a first temperature zone with a first temperature and a second temperature zone with a second temperature.

4. The biological reagent detection device according to claim 3, characterized in that: The temperature control component is slidably connected to the loading component, and the reagent alternately exchanges heat with the first temperature zone and the second temperature zone.

5. The biological reagent detection device according to claim 4, characterized in that: Both the first temperature zone and the second temperature zone have heat exchange chambers, and the reagents built into the loading assembly are located in the heat exchange chambers.

6. The biological reagent detection device according to claim 1, characterized in that: The optical components include a light receiving unit that receives the light emitted by the reagent.

7. The biological reagent detection device according to claim 1, characterized in that: The optical components include a light output unit that inputs excitation light into the reagent.

8. The biological reagent detection device according to claim 7, characterized in that: The light output unit includes a light source, and there are at least two light sources.

9. A biological reagent detection device according to claim 8, characterized in that: Each of the light sources emits light at an independent wavelength.

10. A biological reagent detection device according to claim 1, characterized in that: The light analysis component includes a light analysis chip.

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