An organoid-organ-on-a-chip optical imaging and irradiation fixation device

Abstract

This utility model relates to the field of organoid-organ-on-a-chip imaging technology, and provides an organoid-organ-on-a-chip optical imaging and irradiation fixation device, including a fixing frame adapted to the size of the organoid-organ-on-a-chip and a pressing frame that is snapped onto the fixing frame. Both the fixing frame and the pressing frame are provided with windows for optical imaging of the organoid-organ-on-a-chip. When fixing the organoid-organ-on-a-chip, the organoid-organ-on-a-chip is first placed in the fixing frame, and then the pressing frame is snapped onto the fixing frame to achieve fixation of the organoid-organ-on-a-chip. The fixing frame is adapted to the size of the organoid-organ-on-a-chip, the structure is simple, the operation is convenient, and it can effectively fix the organoid-organ-on-a-chip, which has good practical significance.

Description

Technical Field

[0001] This utility model relates to the field of organoid-organ-on-a-chip imaging technology, specifically an organoid-organ-on-a-chip optical imaging and irradiation fixation device. Background Technology

[0002] Organoid-on-a-chip is an experimental platform that uses microfluidic technology to simulate the structure and function of human organs. This chip contains tiny channels and chambers, and by controlling parameters such as culture medium flow, mechanical force, and concentration gradients of biochemical substances, it can simulate the three-dimensional culture microenvironment of real human organs. By precisely controlling the flow of fluids and environmental conditions, organoids can be cultured on the chip to study interactions between multiple cell types, drug delivery, and pathological states. Organoid-on-a-chip has been widely used in drug screening, toxicology testing, disease model research, and cosmetic safety and efficacy evaluation.

[0003] Bio-optical imaging is widely used due to its mature detection instruments, high sensitivity, high contrast, high resolution, intuitive imaging, fast imaging speed, and non-destructive testing. It has important practical significance and application prospects in exploring the pathogenesis, clinical manifestations, and gene mutations of diseases, understanding the corresponding physiological and pathological information, disease diagnosis, and the development of new medical methods. Currently, when irradiating based on the results of bio-optical imaging of organoids-organ-on-a-chip, the existing fixation devices have the defect of being incompatible with organoid-organ-on-a-chip, making them inconvenient to operate and resulting in poor fixation effect on organoid-organ-on-a-chip.

[0004] Therefore, this invention proposes an organoid-organ-on-a-chip optical imaging and irradiation fixation device to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide an organoid-organ-on-a-chip optical imaging and irradiation fixation device to solve the above-mentioned problems.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] An organoid-organ-on-a-chip optical imaging and irradiation fixation device includes a fixation frame adapted to the size of the organoid-organ-on-a-chip and a pressure frame that is snapped into the fixation frame. Both the fixation frame and the pressure frame are provided with windows for optical imaging of the organoid-organ-on-a-chip.

[0008] In one alternative: the pressure frame is hinged to the fixed compartment frame, and the fixed compartment frame and the pressure frame are locked together by a snap fastener.

[0009] In one alternative: the buckle includes a male buckle disposed on the fixed frame and a female buckle disposed on the pressure frame and adapted to the male buckle.

[0010] In one alternative: the fixing device further includes a base, and the fixing frame is disposed on the base.

[0011] In one alternative: the fixture further includes a storage chamber disposed on the base for housing a supply pump to maintain nutrient supply to the organoid-organ-on-a-chip.

[0012] In one alternative: the containment compartment includes a body and a cover, the cover having several openings.

[0013] In one alternative: the cabin is in the form of an inner recess.

[0014] In one alternative: the pressure frame is further provided with several slots for holding the supply pipe in place.

[0015] In one alternative: the bottom of the base is further provided with a protrusion that corresponds to and fits the socket on the turntable.

[0016] Compared with the prior art, the beneficial effects of this utility model embodiment are as follows:

[0017] 1. When fixing organoid-organ-chip, first place the organoid-organ-chip into the fixing chamber frame, and then press the pressure frame onto the fixing chamber frame to fix the organoid-organ-chip. The fixing chamber frame is adapted to the size of the organoid-organ-chip, the structure is simple and the operation is convenient. It can effectively fix the organoid-organ-chip and has good practical significance.

[0018] 2. By placing the supply pump in the storage chamber, the organoid-organ-on-a-chip fixation operation is not affected, and a stable nutrient supply can be provided during the organoid-organ-on-a-chip optical imaging and irradiation process.

[0019] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit this application. Attached Figure Description

[0020] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this application and, together with the description, serve to explain the principles of this application. Furthermore, these drawings and textual descriptions are not intended to limit the scope of the concept in any way, but rather to illustrate the concepts of this application to those skilled in the art through reference to specific embodiments.

[0021] Figure 1 This is a structural schematic diagram of an embodiment of the present utility model.

[0022] Figure 2 This is a schematic diagram of another embodiment of the present invention.

[0023] Attached diagram annotations: 1-base, 2-fixed compartment frame, 3-pressure frame, 4-hinge, 5-bayonet, 6-female buckle, 7-male buckle, 8-accommodation compartment, 9-protrusion. Detailed Implementation

[0024] The present application will now be described in further detail with reference to the accompanying drawings. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0025] Please see Figure 1 An organoid-organ-on-a-chip optical imaging and irradiation fixation device includes a fixation frame 2 adapted to the size of the organoid-organ-on-a-chip and a pressure frame 3 that is snapped into the fixation frame 2. Both the fixation frame 2 and the pressure frame 3 are provided with windows for optical imaging of the organoid-organ-on-a-chip.

[0026] When fixing organoid-organ-on-a-chip, the organoid-organ-on-a-chip is first placed in the fixing frame 2, and then the pressure frame 3 is pressed onto the fixing frame 2 to fix the organoid-organ-on-a-chip. The fixing frame 2 is adapted to the size of the organoid-organ-on-a-chip, the structure is simple and the operation is convenient, and it can effectively fix the organoid-organ-on-a-chip, which has good practical significance.

[0027] Furthermore, the fixing frame 2 can be set vertically, horizontally, or at other angles, depending on the actual fixing requirements of the organoid-organ chip. This application does not make any special limitations here.

[0028] Please see Figure 1 In one embodiment of this utility model, the pressure frame 3 is hinged to the fixed compartment frame 2 by a hinge 4, and the fixed compartment frame 2 and the pressure frame 3 are locked together by a buckle. The fixed compartment frame 2 is hinged to the fixed compartment frame 2 to facilitate fastening. When fastened, it is locked by a buckle.

[0029] Furthermore, in this embodiment, the buckle includes a male buckle 7 disposed on the fixed frame 2 and a female buckle 6 disposed on the pressure frame 3 and adapted to the male buckle 7.

[0030] Please see Figure 1 In one embodiment of the present invention, the fixing device further includes a base 1, and the fixing frame 2 is disposed on the base 1. The fixing device can be placed as a whole on the turntable, so that it can rotate with the turntable to facilitate optical imaging and irradiation operations.

[0031] Based on the previous embodiment, please refer to Figure 2 In one embodiment of the present invention, the bottom of the base 1 is further provided with a protrusion 9 that corresponds to and is adapted to the insertion hole on the turntable. When the fixing device is placed on the turntable, the protrusion 9 at the bottom of the base 1 is inserted into the corresponding insertion hole on the turntable to limit the fixing device and ensure the stability during the rotation imaging and irradiation process.

[0032] Please see Figure 1 In one embodiment of the present invention, the fixing device further includes a container 8 disposed on the base 1 for placing a supply pump to maintain the nutrient supply to the organoid-organ chip.

[0033] The storage compartment 8 includes a compartment body and a cover, and the cover has several openings.

[0034] It should be noted that the supply pump (i.e., a microfluidic pump, external) is adapted to the organoid-organ-on-a-chip, and several supply channels (i.e., microfluidic channels, usually flexible tubes) are provided between the supply pump and the organoid-organ-on-a-chip. The supply pump is used to drive the flow of liquid and gas to maintain the supply of nutrients and oxygen to the organoid-organ-on-a-chip. In addition, in this embodiment, the cover can be hinged to the cabin or directly closed onto the cabin, as long as the cover can be opened and closed. This embodiment does not limit the specific arrangement between the cover and the cabin.

[0035] In this embodiment, during the organ-organ-on-a-chip fixation operation, it is necessary to remove the supply pump and corresponding supply pipes connected to the organ-organ-on-a-chip to avoid dragging and obstructing the fixation, which would prevent the organ-organ-on-a-chip from receiving nutrition during optical imaging and irradiation. To solve this problem, this embodiment provides a housing chamber 8. When fixing the organ-organ-on-a-chip, the supply pump is placed in the housing chamber 8, which does not affect the organ-organ-on-a-chip fixation operation and can provide a stable nutrition supply during the organ-organ-on-a-chip optical imaging and irradiation process. Specifically, the supply pump is placed in the chamber and the supply pipes are arranged so that the supply pipes correspond to the notches on the chamber cover (one supply pipe corresponds to one notch), so that the supply pipes are not crushed when the chamber cover is closed, ensuring normal fluid flow.

[0036] Furthermore, in this embodiment, the cabin is in the form of an inner recess.

[0037] Furthermore, in this embodiment, the pressure frame 3 is also provided with a number of slots 5 for holding the supply pipe. The supply pipe is placed into the slot 5 (one slot 5 corresponds to one supply pipe) to limit its position, so as to prevent the pipe sections located outside the cabin from being dragged and falling messily.

[0038] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An organoid-organ-on-a-chip optical imaging and irradiation fixation device, characterized in that, It includes a fixed frame (2) adapted to the size of organoid-organ-chip and a pressing frame (3) that is snapped into the fixed frame (2). Both the fixed frame (2) and the pressing frame (3) are provided with windows for optical imaging of organoid-organ-chip.

2. The organoid-organ-on-a-chip optical imaging and irradiation fixation device according to claim 1, characterized in that, The pressure frame (3) is hinged to the fixed compartment frame (2) by a hinge (4), and the fixed compartment frame (2) and the pressure frame (3) are locked together by a buckle.

3. The organoid-organ-on-a-chip optical imaging and irradiation fixation device according to claim 2, characterized in that, The buckle includes a male buckle (7) provided on the fixed frame (2) and a female buckle (6) provided on the pressure frame (3) and adapted to the male buckle (7).

4. The organoid-organ-on-a-chip optical imaging and irradiation fixation device according to claim 1, characterized in that, The fixing device also includes a base (1), and the fixing frame (2) is disposed on the base (1).

5. The organoid-organ-on-a-chip optical imaging and irradiation fixation device according to claim 4, characterized in that, The fixing device also includes a container (8) disposed on the base (1) for placing the supply pump to maintain the nutrient supply to the organoid-organ chip.

6. The organoid-organ-on-a-chip optical imaging and irradiation fixation device according to claim 5, characterized in that, The storage compartment (8) includes a compartment body and a cover, and the cover has several openings.

7. The organoid-organ-on-a-chip optical imaging and irradiation fixation device according to claim 6, characterized in that, The cabin is in the form of an inner recess.

8. The organoid-organ-on-a-chip optical imaging and irradiation fixation device according to claim 5, characterized in that, The pressure frame (3) is also provided with several slots (5) for holding the supply pipe.

9. The organoid-organ-on-a-chip optical imaging and irradiation fixation device according to claim 4, characterized in that, The bottom of the base (1) is also provided with a protrusion (9) that corresponds to and fits the socket on the turntable.

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