Centrifugal purification device

Abstract

This disclosure provides a centrifugal purification apparatus, comprising a centrifuge cylinder, a suction assembly, and a centrifuge tube for holding lysate. The centrifuge cylinder is located within the centrifuge tube, with a gap between the bottom of the centrifuge cylinder and the bottom of the centrifuge tube, and the top of the centrifuge cylinder is connected to the centrifuge tube. The bottom of the centrifuge cylinder is covered with an oleophobic film. The suction assembly is used to suction the liquid inside the centrifuge tube after centrifugation in the centrifuge tube, positioned within the centrifuge cylinder and passing through the oleophobic film. The centrifugal purification apparatus provided by this disclosure can improve the purity of the supernatant.

Description

Technical Field

[0001] This disclosure relates to the field of biotechnology, and in particular to a centrifugal purification apparatus. Background Technology

[0002] In biomedical research, the extraction of supernatant from lysed adipose tissue is a common experimental procedure. The extracted supernatant can be used for subsequent biomolecular analysis.

[0003] In related techniques, centrifuge tubes are generally used to extract the supernatant. First, the lysate of adipose tissue is placed in a centrifuge tube. Then, the centrifuge tube is placed in a high-speed centrifuge for centrifugation. Due to the density differences of different components, the lysate in the centrifuge tube will separate into layers: the upper layer is the desired supernatant, and the lower layer is a precipitate layer formed by cell debris and insoluble substances. Finally, the supernatant is obtained by pipetting the upper layer.

[0004] However, because the lysate from adipose tissue contains a large amount of oil, during high-speed centrifugation, the oil forms a layer on top of the supernatant. Therefore, when the pipette tip aspirates the supernatant, it also picks up the oil, resulting in an impure supernatant and affecting the accuracy of the experimental results. Utility Model Content

[0005] This disclosure provides a centrifugal purification apparatus that can improve the purity of the supernatant. The technical solution is as follows:

[0006] This disclosure provides a centrifugal purification apparatus, which includes a centrifuge cylinder, a suction assembly, and a centrifuge tube for holding lysate. The centrifuge cylinder is located inside the centrifuge tube, and there is a gap between the bottom of the centrifuge cylinder and the bottom of the centrifuge tube. The top of the centrifuge cylinder is connected to the centrifuge tube, and the bottom of the centrifuge cylinder has an opening covered with an oleophobic film. The suction assembly is used to suction the liquid inside the centrifuge tube after centrifugation in the centrifuge tube, which is located inside the centrifuge cylinder and passes through the oleophobic film.

[0007] In another implementation of this disclosure, the centrifuge tube includes a column body and a nozzle section, the bottom of the column body is connected to one end of the nozzle section, the outer diameter of the nozzle section is less than or equal to the outer diameter of the column body, and the oleophobic film covers the other end of the nozzle section.

[0008] In another implementation of this disclosure, the centrifuge tube further includes an outer flange located on the outer wall of the column body near the top and connected to the outer wall of the column body, and the outer flange is interference-fitted with the inner wall of the centrifuge tube.

[0009] In another implementation of this disclosure, the distance between the tube opening and the bottom of the centrifuge tube is not less than 1 / 5 of the length of the centrifuge tube.

[0010] In another implementation of this disclosure, the centrifuge cylinder further includes a cap and a hinged arm, one end of which is connected to the outer peripheral wall of the cap, and the other end of which is connected to the outer flange. One end of the hinged arm is capable of flipping relative to the other end so that the cap covers the top of the cylinder body.

[0011] In another implementation of this disclosure, the folding arm is a plastic strip-shaped structural member with a crease in the middle.

[0012] In another implementation of this disclosure, the centrifuge cylinder is an integrally injection-molded structural component.

[0013] In another implementation of this disclosure, the oleophobic film is one of polytetrafluoroethylene oleophobic film, perfluoropolyether, fluorinated ethylene propylene copolymer, and polydimethylsiloxane.

[0014] In another implementation of this disclosure, the thickness of the oleophobic film is no greater than 100 μm.

[0015] In another implementation of this disclosure, the aspiration component is a syringe.

[0016] The beneficial effects of the technical solutions provided in this disclosure are:

[0017] When purifying the lysate using the centrifugal purification apparatus provided in this embodiment, the lysate is first loaded into centrifuge tubes. Then, a centrifuge cylinder coated with an oleophobic membrane is assembled into the centrifuge tubes. The centrifuge tubes containing the centrifuge cylinder are then placed in a centrifuge for centrifugation. During centrifugation, due to the lower density of oils, they float on the surface of the supernatant. Furthermore, due to the oleophobic nature of the membrane, the oils gradually adhere to the outer surface of the centrifuge cylinder, avoiding the membrane, thus effectively separating the oils and supernatant. After centrifugation, the supernatant can be drawn through the oleophobic membrane using a suction assembly. Because of the oleophobic membrane, the suction assembly only draws up the supernatant, without any oils.

[0018] As can be seen, the centrifugal purification device provided in the above-disclosed embodiments, by placing a special centrifuge tube inside the centrifuge tube and utilizing an oleophobic membrane in conjunction with high-speed centrifugation, can more effectively separate oils and supernatants, solving the problem that oils are easily drawn by the pipette tip, improving the extraction purity of the supernatant, and reducing protein degradation and the loss of other biomolecules, thus providing a purer sample for subsequent biomolecular analysis. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this disclosure, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this disclosure. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of a centrifugal purification apparatus provided in an embodiment of the present disclosure;

[0021] Figure 2 for Figure 1 Schematic diagram of the structure of the centrifuge tube;

[0022] Figure 3 for Figure 1 Schematic diagram of the structure of a centrifuge tube;

[0023] Figure 4 for Figure 1 A schematic diagram of the structure of a centrifugal syringe.

[0024] The symbols in the diagram represent the following meanings:

[0025] 1. Centrifuge cylinder; 11. Column body; 12. Nozzle section; 13. Outer flange; 14. Cylinder cap; 142. Inner groove; 15. Folding arm; 16. Inner flange; 20. Oleophobic film;

[0026] 2. Suction assembly;

[0027] 3. Centrifuge tube; 31. Tube body; 32. Tube cap. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of this disclosure clearer, the embodiments of this disclosure will be described in further detail below with reference to the accompanying drawings.

[0029] This disclosure provides a centrifugal purification apparatus, such as... Figure 1 As shown, the centrifugal purification device includes a centrifuge cylinder 1, a suction assembly 2, and a centrifuge tube 3 for holding the lysate.

[0030] Centrifuge cylinder 1 is located inside centrifuge tube 3. There is a gap between the bottom of centrifuge cylinder 1 and the bottom of centrifuge tube 3, and the top of centrifuge cylinder 1 is connected to centrifuge tube 3. The bottom of centrifuge cylinder 1 is covered with an oleophobic film 20. The suction assembly 2 is used to suction the liquid in centrifuge tube 3 after centrifugation is completed, located inside centrifuge cylinder 1 and passing through the oleophobic film 20.

[0031] When purifying the lysate using the centrifugal purification apparatus provided in this embodiment, the lysate is first loaded into a centrifuge tube 3. Then, a centrifuge cylinder 1 coated with an oleophobic membrane 20 is assembled into the centrifuge tube 3. Next, the centrifuge tube 3 containing the centrifuge cylinder 1 is placed in a centrifuge for centrifugation. During centrifugation, due to the lower density of oil, it floats on top of the supernatant. Furthermore, due to the oleophobic nature of the oleophobic membrane 20, the oil gradually adheres to the outer surface of the centrifuge cylinder 1, avoiding the oleophobic membrane 20, thus effectively separating the oil and supernatant. After centrifugation, the supernatant is adsorbed by the suction assembly 2 through the oleophobic membrane 20. Due to the effect of the oleophobic membrane 20, primarily the supernatant is absorbed, with no oil remaining.

[0032] For example, when the height of the lysis buffer is higher than the height of the oleophobic membrane, the grease adheres to the outer surface of the centrifuge tube 1; while when the height of the lysis buffer is lower than the height of the oleophobic membrane, the grease will still adhere to the upper layer of the supernatant. Moreover, the above structure can also supplement the benefits of low-temperature centrifugation, which can cause the grease to solidify and become less fluid, making it easier for the suction component 2 to draw up the supernatant, and can also alleviate the degradation of proteins and nucleic acids.

[0033] As can be seen, the centrifugal purification device provided in the above-disclosed embodiments, by placing a special centrifuge tube 1 inside the centrifuge tube 3 and using an oleophobic membrane 20 in conjunction with high-speed centrifugation, can more effectively separate oils and supernatants, solving the problem that oils are easily drawn by the pipette tip in the prior art, improving the extraction purity of the supernatant, and reducing protein degradation and the loss of other biomolecules, thus providing a purer sample for subsequent biomolecular analysis.

[0034] Figure 2 for Figure 1 The structural diagram of the centrifuge tube in the diagram, combined with Figure 2 Optionally, the centrifuge tube 1 includes a column body 11 and a nozzle section 12. The bottom of the column body 11 is connected to one end of the nozzle section 12. The top of the column body 11 is connected to the centrifuge tube 3.

[0035] The outer diameter of the nozzle section 12 is less than or equal to the outer diameter of the column body 11, and the oleophobic film 20 covers the other end of the nozzle section 12.

[0036] In the above implementation, the centrifuge tube 1 is configured as a column body 11 and a tube opening section 12. This allows a larger space to be formed between the tube opening section 12 with a smaller outer diameter and the centrifuge tube 3, so that the grease in the lysate after centrifugation can adhere to the outer surface of the tube opening section 12.

[0037] In other words, by using the smaller outer diameter pipe section 12, the space between the pipe section 12 and the centrifuge tube 3 can be increased, so that the grease after centrifugation can easily adhere to the outer surface of the centrifuge tube 1.

[0038] In this embodiment, the end of the pipe section 12 facing the column body 11 is a flared section. The inner diameter of the flared section gradually decreases along the direction from the column body 11 to the pipe section 12. This allows the pipe section 12 to be quickly and effectively connected to the column body 11 through the flared section.

[0039] Meanwhile, to facilitate the suction assembly 2's suction of the supernatant after passing through the oleophobic membrane 20, the opening is located at the end of the tube section 12 furthest from the column body 11. When the suction assembly 2 suctions the supernatant, it is located inside the centrifuge cylinder 1, with the suction end puncturing the oleophobic membrane 20. This allows the suction assembly 2 to smoothly suction the supernatant.

[0040] Optionally, the column body 11 and the centrifuge tube 3 are fitted with a clearance.

[0041] In the above implementation, the gap fit between the column body 11 and the centrifuge tube 3 facilitates the insertion of the centrifuge tube 1 into the centrifuge tube 3 and makes the assembly of the centrifuge tube 1 convenient.

[0042] Optionally, the centrifuge tube 1 also includes an outer flange 13, which is located at the top of the column body 11 and connected to the outer wall of the column body 11. The outer flange 13 is press-fitted with the inner wall of the centrifuge tube 3.

[0043] In the above implementation, the outer flange 13 facilitates the centrifuge cylinder 1 to be fitted into the centrifuge tube 3, so that the centrifuge cylinder 1 can be suspended at the top of the centrifuge tube 3.

[0044] In other examples, the centrifuge tube 1 can also be connected to the centrifuge tube 3 in other ways, such as by providing an inner flange that mates with the outer flange 13 on the inner wall of the centrifuge tube 3.

[0045] Optionally, the centrifuge cylinder 1 also includes a cylinder cover 14 and a hinge arm 15. One end of the hinge arm 15 is connected to the outer peripheral wall of the cylinder cover 14, and the other end of the hinge arm 15 is connected to the outer flange 13. One end of the hinge arm 15 can be flipped relative to the other end so that the cylinder cover 14 seals the top of the cylinder body 11.

[0046] In this embodiment, the folding arm 15 is a plastic strip-shaped structural component with creases. This allows the cap 14 to be effectively connected to the outer flange 13 via the folding arm 15, and enables the cap 14 to flip relative to the cylindrical body 11.

[0047] In addition, to facilitate the positioning of the cap 14 within the column body 11, the inner wall of the column body 11 has an inner flange 16, and the outer periphery of the cap 14 has an inner groove 142. The groove wall of the inner groove 142 overlaps with the inner flange 16, which facilitates the positioning of the cap 14.

[0048] Optionally, the distance from the bottom of the tube end section 12 to the bottom of the centrifuge tube 3 ( Figure 1 The midpoint distance L1 is not less than the length from the top to the bottom of centrifuge tube 3. Figure 1 1 / 5 of the mid-range L2.

[0049] In the above implementation, the distance between the tube opening section 12 and the bottom of the centrifuge tube 3 is set to be no less than 1 / 5 of the length of the centrifuge tube 3, so that there is enough space between the centrifuge tube 3 and the centrifuge cylinder 1 to assemble the lysis solution.

[0050] Figure 3 for Figure 1 A schematic diagram of the structure of a centrifuge tube, combined with... Figure 3 The centrifuge tube 3 includes a tube body 31 and a tube cap 32. The tube cap 32 is connected to the top of the tube body 31 by a folding arm, and the tube cap 32 and the tube body 31 are connected together by a flange and an inner groove structure similar to the tube cap 14 and the column body 11 of the centrifuge cylinder 1 (details will not be elaborated here). In this way, the tube body 31 can be sealed by the tube cap 32, which can be adapted to centrifugation with greater centrifugal force.

[0051] In this embodiment, the centrifuge tube 3 can be a 1.5 or 2 ml plastic centrifuge tube. This not only facilitates the processing of the centrifuge tube 1, but also reduces the cost of the centrifuge tube 1.

[0052] In this embodiment, the centrifuge tube 1 is a polypropylene structural component. This allows the centrifuge tube to have good biocompatibility and chemical stability.

[0053] Furthermore, to facilitate the manufacturing of centrifuge cylinder 1, centrifuge cylinder 1 is a one-piece injection molded structural component. That is, the oleophobic film 20 is integrally molded during the molding process of centrifuge cylinder 1. Moreover, centrifuge cylinder 1 can also be replaced by commercially available centrifuge columns.

[0054] Optionally, the oleophobic film 20 is one of polytetrafluoroethylene, perfluoropolyether, fluorinated ethylene propylene copolymer, and polydimethylsiloxane.

[0055] In this embodiment, the oleophobic membrane 20 is a polytetrafluoroethylene membrane. This allows the oleophobic membrane 20 to withstand all organic solvents (such as alcohols, acetone, and hydrocarbons) and strong acids and alkalis (including aqua regia and concentrated sulfuric acid), making it suitable for treating corrosive oily samples.

[0056] Furthermore, the oleophobic membrane 20 does not react chemically with oils or reagents, thus preventing sample contamination (e.g., preventing adsorption loss during nucleic acid and protein extraction). Additionally, polytetrafluoroethylene effectively repels oils and water, reducing residue and causing the separated oils to accumulate on the outer surface of the centrifuge tube 1.

[0057] Optionally, the thickness of the oleophobic film 20 is no greater than 100 μm. This allows the oleophobic film 20 to have good flexibility and be able to stably coat the bottom of the centrifuge tube 1.

[0058] In this embodiment, the thickness of the oleophobic film 20 can be 20-30 μm. This allows the oleophobic film 20 to have sufficiently good toughness, etc.

[0059] Figure 4 for Figure 1 A schematic diagram of the structure of a centrifugal syringe, combined with Figure 4 Optionally, the suction component 2 is a syringe.

[0060] In the above implementation, the suction component 2 is set as a syringe, so that the supernatant can be easily aspirated by the syringe, and the oleophobic film can be easily punctured by the syringe needle, thereby facilitating the suction of the supernatant.

[0061] Optionally, the volume of the syringe shall not exceed the volume of the centrifuge tube 3.

[0062] In the above implementation, the volume of the syringe does not exceed the volume of the centrifuge tube 3, which ensures that the syringe does not aspirate the supernatant too quickly, effectively controlling the aspiration speed and preventing the sediment layer from being aspirated into the syringe.

[0063] For example, the syringe has a volume of 1 ml.

[0064] The following is a brief introduction to the usage process of the centrifugal purification apparatus provided in this embodiment:

[0065] During centrifugation, the lysate is first loaded into centrifuge tube 3. Then, centrifuge cylinder 1, which is coated with an oleophobic film 20, is assembled into centrifuge tube 3.

[0066] Then, place the centrifuge tube containing centrifuge cylinder 1 into a centrifuge for centrifugation.

[0067] During centrifugation, the low temperature and high speed cause the oil to float on the top of the supernatant due to its low density. Furthermore, the oil-repellent properties of the oleophobic membrane 20 allow the oil to move away from the membrane and gradually adhere to the outer surface of the centrifuge cylinder 1, while the membrane 20 remains free of oil, effectively separating the oil and the supernatant.

[0068] After centrifugation, insert the syringe through the oleophobic membrane 20 and aspirate the supernatant. Due to the oleophobic membrane, only the supernatant is aspirated, with no grease present.

[0069] Therefore, the centrifugal purification device provided in the above-disclosed embodiments, by placing a special centrifuge tube 1 inside the centrifuge tube 3 and using an oleophobic membrane 20 in conjunction with high-speed centrifugation, can more effectively separate oils and supernatants, solving the problem that oils are easily drawn by the pipette tip in the prior art, improving the extraction purity of the supernatant, and reducing protein degradation and the loss of other biomolecules, thus providing a purer sample for subsequent biomolecular analysis.

[0070] The above are merely optional embodiments of this disclosure and are not intended to limit this disclosure. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this disclosure should be included within the protection scope of this disclosure.

Claims

1. A centrifugal purification apparatus, characterized in that, The centrifugal purification device includes a centrifuge cylinder (1), a suction assembly (2), and centrifuge tubes (3) for holding the lysate. The centrifuge tube (1) is located in the centrifuge tube (3). There is a gap between the bottom of the centrifuge tube (1) and the bottom of the centrifuge tube (3). The top of the centrifuge tube (1) is connected to the centrifuge tube (3). The bottom of the centrifuge tube (1) is covered with an oleophobic film (20). The suction assembly (2) is used to suction the liquid in the centrifuge tube (3) after centrifugation is completed, located inside the centrifuge cylinder (1) and passing through the oleophobic membrane (20).

2. The centrifugal purification apparatus according to claim 1, characterized in that, The centrifuge tube (1) includes a column body (11) and a nozzle section (12). The bottom of the column body (11) is connected to one end of the nozzle section (12). The outer diameter of the nozzle section (12) is less than or equal to the outer diameter of the column body (11). The oleophobic film (20) covers the other end of the nozzle section (12).

3. The centrifugal purification apparatus according to claim 2, characterized in that, The centrifuge tube (1) also includes an outer flange (13), which is located at the top of the column body (11) and connected to the column body (11). The outer flange (13) is interference-fitted with the inner wall of the centrifuge tube (3).

4. The centrifugal purification apparatus according to claim 2, characterized in that, The distance between the pipe opening section (12) and the bottom of the centrifuge tube (3) is not less than 1 / 5 of the length of the centrifuge tube (3).

5. The centrifugal purification apparatus according to claim 3, characterized in that, The centrifuge tube (1) also includes a tube cover (14) and a hinge arm (15). One end of the hinge arm (15) is connected to the outer peripheral wall of the tube cover (14), and the other end of the hinge arm (15) is connected to the outer flange (13). One end of the hinge arm (15) can be flipped relative to the other end so that the tube cover (14) covers the top of the column body (11).

6. The centrifugal purification apparatus according to claim 5, characterized in that, The folding arm (15) is a plastic strip-shaped structural component with a fold in the middle.

7. The centrifugal purification apparatus according to claim 5, characterized in that, The centrifuge tube (1) is an integral injection-molded structural component.

8. The centrifugal purification apparatus according to any one of claims 1-7, characterized in that, The oleophobic film (20) is one of polytetrafluoroethylene, perfluoropolyether, fluorinated ethylene propylene copolymer, and polydimethylsiloxane.

9. The centrifugal purification apparatus according to any one of claims 1-7, characterized in that, The thickness of the oleophobic film (20) is no greater than 100 μm.

10. The centrifugal purification apparatus according to any one of claims 1-7, characterized in that, The suction component (2) is a syringe.

Images