A nucleic acid extractor

Abstract

This utility model relates to the field of nucleic acid detection technology, specifically disclosing a nucleic acid extractor, including a nucleic acid extractor body. Multiple magnetic rod sleeves are disposed within the nucleic acid extractor body. Two fixing plates are fixedly connected to the nucleic acid extractor body, and a rotating shaft is disposed on each of the two fixing plates. One end of the rotating shaft is rotatably connected to one of the fixing plates, and the other end of the rotating shaft passes through the other fixing plate. A first motor is disposed on the nucleic acid extractor body, and the power output shaft of the first motor is fixedly connected to the end of the rotating shaft passing through the fixing plate. An automatic magnetic rod sleeve unloading device is disposed on the rotating shaft for automatically removing the used magnetic rod sleeves, thus solving the technical problem that existing nucleic acid extractors cannot automatically remove used magnetic rod sleeves.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid detection technology, and specifically discloses a nucleic acid extractor. Background Technology

[0002] Nucleic acid extractors are key equipment for the automated separation of nucleic acids from biological samples. Their core principle is to capture and purify nucleic acids through magnetic bead adsorption. In this technology system, magnetic rods act as magnetic force carriers, driving the magnetic beads to transfer between steps such as lysis, binding, washing, and elution through vertical lifting and lowering motion. To ensure operational safety and avoid cross-contamination, the magnetic rods must be covered with disposable magnetic rod sleeves. This device is made of biocompatible plastic and has both magnetic field conduction and physical isolation functions. The existing equipment's workflow is as follows: when the magnetic rod assembly is lowered to the deep well plate, the magnetic rod sleeve is immersed in the sample liquid containing magnetic beads. The magnetic field activates the magnetic beads to adsorb onto the outer wall of the sleeve. After extraction is completed and the magnetic rod assembly is raised to the high position, the operator must manually remove the magnetic rod sleeve and discard it.

[0003] In existing nucleic acid extractors, the magnetic rod sleeve is removed manually by the operator during the final removal process. This process causes the operator to frequently come into contact with the magnetic rod sleeve carrying pathogens, which poses a risk of virus exposure and cross-contamination. High-throughput equipment requires disassembly of each hole, which is time-consuming and disconnected from the automated process. Manual pulling can easily cause the magnetic rod sleeve to break or the fixing mechanism to be damaged, increasing the risk of failure. Utility Model Content

[0004] In view of this, the purpose of this utility model is to provide a nucleic acid extractor to solve the technical problem that existing nucleic acid extractors cannot automatically remove the used magnetic rod sleeve.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a nucleic acid extractor, comprising a nucleic acid extractor body, wherein a plurality of magnetic rod sleeves are disposed within the nucleic acid extractor body, and two fixing plates are fixedly connected to the nucleic acid extractor body, wherein a rotating shaft is disposed on the two fixing plates, one end of the rotating shaft is rotatably connected to one of the fixing plates, and the other end of the rotating shaft passes through the other fixing plate, wherein a first motor is disposed on the nucleic acid extractor body, and the power output shaft of the first motor is fixedly connected to the end of the rotating shaft passing through the fixing plate, and an automatic magnetic rod sleeve unloading device is disposed on the rotating shaft for automatically removing the used magnetic rod sleeves.

[0006] In this solution, the first motor drives the rotating shaft to rotate, which in turn drives the automatic magnetic rod sleeve unloading device to operate, realizing the function of automatically removing the magnetic rod sleeve after use. The automatic magnetic rod sleeve unloading device can automatically unload and remove the magnetic rod sleeve from the nucleic acid extractor body. The whole process does not require manual contact with the magnetic rod sleeve, effectively avoiding the risk of bacterial contamination that may be caused by manual operation, and improving the safety and hygiene of use.

[0007] Furthermore, the automatic unloading device for the magnetic rod sleeve includes an unloading frame, which is mounted on the rotating shaft. The unloading frame has a sliding groove extending through both sides. A slidable sliding plate is provided in the sliding groove. A toothed rail is provided on the sliding plate. An electric push rod is fixedly connected to the sliding plate. The telescopic end of the electric push rod can cooperate with multiple magnetic rod sleeves. A first connecting plate is fixedly connected below the sliding plate. A second connecting plate that can cooperate with the magnetic rod sleeve is installed on the first connecting plate. A driving mechanism for driving the sliding plate to move within the sliding groove is provided above the unloading frame.

[0008] In this solution, the toothed rail on the sliding plate cooperates with the drive mechanism to ensure the precise movement of the sliding plate. The telescopic end of the electric push rod can cooperate with the magnetic rod sleeve. Through the coordinated action of the electric push rod and the second connecting plate, the action of manually pressing to remove the magnetic rod sleeve is simulated, realizing the automatic unlocking and removal of the magnetic rod sleeve. This improves the automation level of the operation, reduces the intensity of manual labor, and effectively avoids the risk of bacterial contamination that may be caused by manual contact with the magnetic rod sleeve.

[0009] Furthermore, the magnetic rod sleeve automatic unloading device also includes a crossbar, which is fixedly connected to the telescopic end of the electric push rod.

[0010] In this solution, the crossbar is fixedly connected to the telescopic end of the electric push rod, allowing multiple magnetic rod sleeves to be pressed and unlocked simultaneously. Through the cooperation of the crossbar and the electric push rod, batch processing of magnetic rod sleeves is achieved, effectively reducing operation time and improving the overall efficiency of nucleic acid extraction.

[0011] Furthermore, the automatic unloading device for the magnetic rod sleeve also includes multiple push rods. The first connecting plate has multiple through holes, and the multiple push rods are fixedly connected to the inner wall of the unloading frame. The multiple push rods can pass through the multiple through holes and contact the multiple magnetic rod sleeves.

[0012] In this scheme, after the crossbar and the second connecting plate complete the magnetic rod sleeve clamping and moving in, the top rod fixed to the inner wall of the unloading frame accurately penetrates the through hole on the first connecting plate and horizontally pushes the magnetic rod sleeve away from the clamping area to complete the automatic unloading.

[0013] Furthermore, the drive mechanism includes a second motor, which is fixedly connected to the unloading frame, and the power output end of the second motor is fixedly connected to a gear that can mesh with the gear rail for transmission.

[0014] In this scheme, when the second motor starts, the gear meshes with the toothed rail and drives the sliding plate to move horizontally synchronously in the sliding groove. Since the magnetic rod sleeve is clamped by the crossbar and the second connecting plate and moves together with the sliding plate, the horizontal movement of the sliding plate directly drives the clamped magnetic rod sleeve to move synchronously into the unloading frame, realizing the automatic unloading of the magnetic rod sleeve.

[0015] Furthermore, the automatic unloading device for the magnetic rod sleeve also includes a collection frame, on which a discharge trough is provided. The collection frame is slidably mounted below the unloading frame, and the collection frame is connected to the discharge trough.

[0016] In this solution, the discharge trough at the bottom of the unloading frame forms a connecting channel with the sliding collection frame. When the magnetic rod sleeve is pushed out by the top rod and detaches from the clamping area of ​​the crossbar and the second connecting plate, it can fall directly into the collection frame along the discharge trough, avoiding the risk of virus spread caused by scattered disposal in traditional manual operation.

[0017] The working principle and beneficial effects of this solution are as follows:

[0018] When the used magnetic rod sleeve needs to be removed from the nucleic acid extractor, the magnetic rod sleeve fixing frame raises the magnetic rod sleeve to a predetermined height, providing a reference for the subsequent positioning of the second connecting plate. Then, the nucleic acid extractor body is opened, and the first motor is started to rotate the shaft 180° counterclockwise, causing the unloading frame to rotate into the body. The electric push rod is then activated, moving the crossbar towards the second connecting plate. Pressing the pressing part of the magnetic rod sleeve simulates manual unlocking, with the second connecting plate providing support and reaction force. Subsequently, the second motor is started, driving the sliding plate to move, and the magnetic rod sleeve is moved into the unloading frame. The sliding plate continues to move, and the push rod pushes the magnetic rod sleeve out, allowing it to fall into the collection frame via the discharge chute. The collection frame slides and locks below the unloading frame, allowing for manual removal when needed. After unloading, the first motor is driven to rotate the shaft 180° clockwise, turning the unloading frame to the side without affecting equipment operation. This solves the technical problem of existing nucleic acid extractors not being able to automatically remove used magnetic rod sleeves.

[0019] Other advantages, objectives, and features of this invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination and study, or may be learned from practice of this invention. The objectives and other advantages of this invention can be realized and obtained through the following description. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of an embodiment;

[0021] Figure 2 This is a schematic diagram of the structure of the nucleic acid extractor body, the magnetic rod sleeve fixing frame, and the magnetic rod sleeve in the embodiment.

[0022] Figure 3 This is a partial cross-sectional view of the magnetic rod sleeve fixing bracket and the magnetic rod sleeve in the embodiment;

[0023] Figure 4 This is a partial sectional view of the automatic unloading device for the magnetic rod sleeve in an embodiment.

[0024] Figure 5 An exploded view of the unloading box, slider, and collection box in an embodiment;

[0025] Figure 6 This is a schematic diagram of the structure of the automatic unloading device for the magnetic rod sleeve in an embodiment.

[0026] The following components are labeled in the attached diagram: 1. Nucleic acid extractor body; 2. Magnetic rod sleeve fixing frame; 3. Magnetic rod sleeve; 4. Two fixing plates; 5. Rotating shaft; 6. Mounting frame; 7. First motor; 8. Connecting plate; 9. Unloading frame; 10. Sliding plate; 11. Electric push rod; 12. First connecting plate; 13. Multiple push rods; 14. Second connecting plate; 15. Sliding groove; 16. Through hole; 17. Crossbar; 18. Second motor; 19. Gear; 20. Gear rail; 21. Square groove; 22. Sliding bar; 23. Collection frame; 24. Mounting groove; 25. Discharge groove. Detailed Implementation

[0027] The following detailed description illustrates the specific implementation method: Example

[0028] like Figures 1 to 6 As shown, a nucleic acid extractor is disclosed, including a nucleic acid extractor body 1, a magnetic rod sleeve fixing frame 2, multiple magnetic rod sleeves 3, two fixing plates 4, a rotating shaft 5, a mounting frame 6, a first motor 7, a connecting plate 8, and an automatic magnetic rod sleeve unloading device. The nucleic acid extractor is equipped with a magnetic rod sleeve fixing frame 2, on which multiple magnetic rod sleeves 3 are slidably mounted. Two fixing plates 4 are fixedly connected to the side wall of the nucleic acid extractor body 1. A rotating shaft 5 is mounted on each fixing plate 4. One end of the rotating shaft 5 is rotatably connected to one of the fixing plates, and the other end of the rotating shaft 5 passes through and rotatably engages with the other fixing plate. The nucleic acid extractor body 1 is fixedly connected to the mounting frame 6, on which a first motor 7 is mounted. The power output shaft of the first motor 7 is fixedly connected to the through end of the rotating shaft 5 via a coupling. A connecting plate 8 is mounted on the rotating shaft 5, located between the two fixing plates 4. An automatic magnetic rod sleeve unloading device is provided at the end of the connecting plate 8 away from the rotating shaft 5, which is used to automatically remove the used magnetic rod sleeves.

[0029] like Figure 2 , Figure 3 and Figure 4As shown, the automatic unloading device for the magnetic rod sleeve includes an unloading frame 9, a sliding plate 10, an electric push rod 11, a first connecting plate 12, multiple push rods 13, a second connecting plate 14, and a drive mechanism. The unloading frame 9 is fixedly connected to the end of the connecting plate 8 away from the rotating shaft 5. A sliding groove 15 is provided on the unloading frame 9, which extends through both sides of the unloading frame 9. The sliding plate 10 is slidably arranged in the sliding groove 15. A toothed rail 20 is provided on the sliding plate 10. The electric push rod 11 is fixedly connected to the end of the sliding plate 10 near the magnetic rod sleeve, and the electric push rod 11 is telescopic. The sliding plate 10 is vertically downward. A first connecting plate 12 is installed on the sliding plate 10. The first connecting plate 12 has multiple through holes 16. Multiple push rods 13 are fixedly connected to the inner wall of the unloading frame 9. The multiple push rods 13 can pass through the multiple through holes 16 and contact the magnetic rod sleeves. A second connecting plate 14 is fixedly connected to the end of the first connecting plate 12 away from the sliding plate 10. The second connecting plate 14 can contact and cooperate with the lower part of the pressing part of the multiple magnetic rod sleeves 3. A driving mechanism is installed on the top of the unloading frame 9. The driving mechanism is used to drive the sliding plate 10 to move in the sliding groove 15.

[0030] like Figure 4 As shown, the automatic unloading device for magnetic rod sleeves also includes a crossbar 17, which is fixedly connected to the telescopic end of the electric push rod 11. The crossbar 17 can contact and cooperate with the upper part of the pressing part of multiple magnetic rod sleeves 3.

[0031] like Figure 4 and Figure 6 As shown, the drive mechanism includes a second motor 18 and a gear 19. A square slot 21 is provided on the unloading frame 9, and the square slot 21 extends into the unloading frame 9. The second motor 18 is fixedly connected to the unloading frame 9. The power output shaft of the second motor 18 is fixedly connected to the gear 19, and the gear 19 passes through the square slot 21 and meshes with the gear rail 20 for transmission.

[0032] like Figure 5 As shown, the automatic unloading device for magnetic rod sleeves also includes two sliding bars 22 and a collection frame 23. Two mounting slots 24 are provided below the unloading frame 9, and a discharge trough 25 is provided on the unloading frame 9. The discharge trough 25 extends into the unloading frame 9. Two sliding bars 22 are slidably engaged in the two mounting slots 24. A collection frame 23 is fixedly connected below the two sliding bars 22, and the collection frame 23 is connected to the discharge trough 25.

[0033] In practice

[0034] When the used magnetic rod sleeve needs to be removed from the nucleic acid extractor body 1, the magnetic rod sleeve fixing frame 2 inside the nucleic acid extractor is first driven to raise the magnetic rod sleeve to a predetermined height, providing a positioning reference for the subsequent placement of the second connecting plate 14 on the magnetic rod sleeve pressing part. Then, the nucleic acid extractor body 1 is opened, and the first motor 7 on the mounting frame 6 is started to drive the rotating shaft 5 on the fixing plate to rotate counterclockwise by 180°. During the rotation, the rotating shaft 5 simultaneously drives the connecting plate 8 to rotate, and the connecting plate 8 drives the unloading frame 9 to rotate into the nucleic acid extractor body 1. After the 180° rotation is completed, the second connecting plate 14 is located below the magnetic rod sleeve pressing part, and the crossbar 17 is located above the magnetic rod sleeve pressing part.

[0035] After the rotating shaft 5 completes a 180° rotation, the electric actuator 11 on the sliding plate 10 is activated. The electric actuator 11 drives the crossbar 17 to move towards the side closer to the second connecting plate 14. During the movement, the crossbar 17 gradually presses the pressing parts of multiple magnetic rod sleeves 3 simultaneously. Due to the elastic buckle arm of the magnetic rod sleeve deforming and contracting under the pressure of the crossbar 17, it stops engaging with the magnetic rod sleeve fixing bracket 2, simulating manual pressing to unlock. At this time, the second connecting plate 14 provides support and reaction force for the pressing of the crossbar 17. When the electric actuator 11 extends and retracts to the predetermined stroke, the electric actuator 11 stops working. The second motor 18 is started to drive the gear 19 to rotate counterclockwise. The gear 19 meshes with the toothed rail 20 on the sliding plate 10 through the square groove 21. When the gear 19 rotates, it will drive the sliding plate 10 to move in the sliding groove 15 opened on the unloading frame 9. Since the second motor 18 drives the gear 19 to rotate counterclockwise, the sliding plate 10 will gradually move away from the magnetic rod sleeve fixing frame 2. During the process, the magnetic rod sleeve is pressed and fixed by the crossbar 17 and the second connecting plate 14. At this time, the magnetic rod sleeve will be moved into the unloading frame 9 at the same time.

[0036] As the magnetic rod sleeve is moved into the unloading frame 9, the first connecting plate 12 on the sliding plate 10 gradually moves closer to the top rod in the unloading frame 9. At this time, the electric push rod 11 gradually starts to drive the crossbar 17 to move away from the end of the second connecting plate 14. The magnetic rod sleeve held by the crossbar 17 gradually ends up being held. As the sliding plate 10 is continuously moved by the gear 19, the top rod pushes the magnetic rod sleeve that has ended up being held out between the crossbar 17 and the second connecting plate 14 through the through hole 16 on the first connecting plate 12. At this time, the magnetic rod sleeve will fall directly into the collection frame 23 through the discharge chute 25 on the unloading frame 9.

[0037] When the collection box 23 slides and is locked in the installation slot 24 below the unloading box 9 by the two sliding bars 22, when it is necessary to remove the collection box 23, you can simply manually remove the collection box 23 from the installation slot 24. At this time, the magnetic rod sleeves collected in the collection box 23 can be processed.

[0038] After the magnetic rod sleeve is automatically unloaded, the first motor 7 is directly driven to rotate the shaft 5 clockwise by 180°. At this time, the unloading frame 9 is on the side of the nucleic acid extractor and will not interfere with the normal use of the nucleic acid extractor.

[0039] The above description is merely an embodiment of this utility model, and common knowledge such as specific structures and characteristics in the solution is not described in detail here. It should be noted that those skilled in the art can make several modifications and improvements without departing from the structure of this utility model, and these should also be considered within the protection scope of this utility model. These modifications and improvements will not affect the effectiveness of the implementation of this utility model or its practicality.

Claims

1. A nucleic acid extractor, characterized in that: The instrument includes a nucleic acid extractor body, which contains multiple magnetic rod sleeves. Two fixed plates are fixedly connected to the nucleic acid extractor body, and a rotating shaft is provided on the two fixed plates. One end of the rotating shaft is rotatably connected to one of the fixed plates, and the other end of the rotating shaft passes through the other fixed plate. A first motor is provided on the nucleic acid extractor body, and the power output shaft of the first motor is fixedly connected to the end of the rotating shaft that passes through the fixed plate. An automatic magnetic rod sleeve unloading device is provided on the rotating shaft for automatically removing the used magnetic rod sleeves.

2. The nucleic acid extractor according to claim 1, characterized in that: The automatic unloading device for magnetic rod sleeves includes an unloading frame mounted on the rotating shaft. The unloading frame has a sliding groove extending through both sides. A slidable sliding plate is disposed within the sliding groove. A toothed rail is provided on the sliding plate. An electric push rod is fixedly connected to the sliding plate. The telescopic end of the electric push rod can cooperate with multiple magnetic rod sleeves. A first connecting plate is fixedly connected below the sliding plate. A second connecting plate, which can cooperate with the magnetic rod sleeves, is mounted on the first connecting plate. A driving mechanism for driving the sliding plate to move within the sliding groove is disposed above the unloading frame.

3. The nucleic acid extractor according to claim 2, characterized in that: The magnetic rod sleeve automatic unloading device also includes a crossbar, which is fixedly connected to the telescopic end of the electric push rod.

4. A nucleic acid extractor according to claim 2, characterized in that: The automatic unloading device for the magnetic rod sleeve also includes multiple push rods. The first connecting plate has multiple through holes, and the multiple push rods are fixedly connected to the inner wall of the unloading frame. The multiple push rods can pass through the multiple through holes and contact the multiple magnetic rod sleeves.

5. A nucleic acid extractor according to claim 2, characterized in that: The drive mechanism includes a second motor, which is fixedly connected to the unloading frame, and the power output end of the second motor is fixedly connected to a gear that can mesh with the gear rail for transmission.

6. A nucleic acid extractor according to claim 2, characterized in that: The automatic unloading device for the magnetic rod sleeve also includes a collection frame, on which a discharge trough is provided. The collection frame is slidably mounted below the unloading frame and is connected to the discharge trough.

Images