petri dish

Abstract

The embodiment of the present application provides a culture dish. The culture dish comprises a dish body, a dish cover and an annular sleeving part, the dish cover is covered on the dish body, the annular sleeving part is arranged on the culture dish, so that the dish body and the dish cover form a seal, at least one air-permeable hole is formed in the dish cover, a waterproof air-permeable film is arranged on the lower surface of the dish cover, the waterproof air-permeable film covers the air-permeable hole, the pore size of the waterproof air-permeable film ranges from 0.2 microns to 0.5 microns, a liquid injection hole is formed in the upper surface of the dish cover, the liquid injection hole penetrates through the dish cover, a plug is arranged in the liquid injection hole, the plug can be inserted into the liquid injection hole, or the plug can be pulled out of the liquid injection hole.

Description

[0001] This application claims priority to Chinese Patent Application No. 202421541404.7, filed on July 1, 2024, entitled “Petri Dish”, the entire contents of which are incorporated herein by reference. Technical Field

[0002] This application relates to the field of cell or microbial culture technology, and more specifically, this application relates to a culture dish. Background Technology

[0003] As containers for culture media, petri dishes have been widely used in the field of cell or microbial culture technology. A petri dish typically consists of a body that holds the culture medium and a matching lid. The lid, placed on top of the body, prevents the culture medium from being contaminated by microorganisms from the external environment, while allowing air to pass through to facilitate the survival, growth, observation, and detection of cells or microorganisms.

[0004] Traditionally, after adding culture medium, culture dishes can only be placed horizontally in an incubator, with cells adhering to the bottom surface of the dish for growth. However, in ground-based simulated microgravity culture, after adding culture medium, the culture dish needs to be placed on a 3D rotating mechanism to achieve 3D rotation. In this way, the cells adhering to the bottom surface of the culture dish can be cultured by rotating the dish in 3D.

[0005] Currently, traditional petri dishes may experience leakage during 3D rotation, as their structure makes 3D rotation culture impossible.

[0006] In view of this, a new technical solution is needed to solve the above-mentioned technical problems. Utility Model Content

[0007] The purpose of this application is to provide a new technology solution for petri dishes.

[0008] In a first aspect, this application provides a petri dish. The petri dish includes: a dish body, a dish lid, and an annular sleeve portion, wherein the dish lid is disposed on the dish body, and the annular sleeve portion is disposed on the petri dish, so that the dish body and the dish lid form a seal;

[0009] The lid of the dish has at least one vent hole, and a waterproof and breathable membrane is provided on the lower surface of the lid, which covers the vent hole. The pore size of the waterproof and breathable membrane is in the range of 0.2μm-0.5μm.

[0010] The upper surface of the lid of the dish has a liquid injection hole that penetrates the lid. A plug is provided inside the liquid injection hole, which can be inserted into or removed from the liquid injection hole.

[0011] Optionally, the lid of the dish has two vent holes, and the lower surface of the lid is provided with two waterproof and breathable membranes, one of which corresponds to one vent hole and the other corresponds to the other vent hole.

[0012] Optionally, the lid has a lid body and a sidewall extending along the outer periphery of the lid body;

[0013] When the lid is placed over the vessel body, there is a gap between the lower surface of the sidewall and the outer surface of the vessel body.

[0014] Optionally, a sealing gasket is provided on the lower surface of the lid body.

[0015] Optionally, the lid includes a lid body, the lid body having a protrusion, and the injection hole penetrating the protrusion and the lid body.

[0016] Optionally, a sealing ring is provided on the outer peripheral wall of the plug;

[0017] When the plug is inserted into the injection hole, the sealing ring abuts against the inner peripheral wall of the injection hole.

[0018] Optionally, the annular sleeve portion includes a first sleeve portion and a second sleeve portion;

[0019] The first sleeve has a first protrusion in the circumferential direction;

[0020] The second set of components is provided with a second protrusion in the circumferential direction;

[0021] When the first sleeve portion is fitted onto the lid of the dish and the second sleeve portion is fitted onto the body of the dish, the first protrusion portion and the second protrusion portion are fastened together.

[0022] Optionally, the petri dish further includes a fastener, wherein the first protrusion has a first threaded hole and the second protrusion has a second threaded hole, and the fastener passes through the first threaded hole and the second threaded hole to fasten the first protrusion and the second protrusion.

[0023] According to an embodiment of this application, a culture dish is provided in which, when the dish lid is placed on the dish body, the dish body and the dish lid are fastened together by an annular sleeve portion, and a seal is formed between the dish body and the dish lid by limiting the pore size of the waterproof and breathable membrane. The culture dish is placed on a 3D rotating mechanism for ground-based microgravity cell culture, thus avoiding leakage of the culture dish.

[0024] Other features and advantages of this specification will become clear from the following detailed description of exemplary embodiments with reference to the accompanying drawings. Attached Figure Description

[0025] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments of this specification and, together with their description, serve to explain the principles of this specification.

[0026] Figure 1 The diagram shows the structure of the petri dish provided in the embodiment of this application. Figure 1 .

[0027] Figure 2 The image shown is a cross-sectional view of the culture dish provided in an embodiment of this application.

[0028] Figure 3 As shown Figure 2 Enlarged view of the structure at point A in the middle.

[0029] Figure 4 The image shown is a cross-sectional view of the culture dish provided in an embodiment of this application.

[0030] Figure 5 As shown Figure 4 Enlarged view of the structure at point B.

[0031] Figure 6 As shown Figure 4 Enlarged view of the structure at point C.

[0032] Figure 7 The diagram shown is a structural diagram of the petri dish and the annular sleeve provided in an embodiment of this application.

[0033] Figure 8 As shown Figure 7 Partial structural diagram of the central annular sleeve section.

[0034] Figure 9 As shown Figure 7 Partial structural diagram of the central annular sleeve section.

[0035] Explanation of reference numerals in the attached figures:

[0036] 1. Petri dish; 11. Dish body; 12. Dish lid; 121. Vent hole; 122. Waterproof and breathable membrane; 123. Injection hole; 124. Plug; 125. Dish lid body; 1251. Sealing gasket; 126. Side wall; 127. Boss part; 1241. Sealing ring; 2. Fastener; 3. Annular sleeve part; 31. First sleeve part; 32. Second sleeve part; 311. First protrusion part; 321. Second protrusion part. Detailed Implementation

[0037] Various exemplary embodiments of the present application will now be described in detail with reference to the accompanying drawings. It should be noted that, unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of the present application.

[0038] The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the scope of this application and its application or use.

[0039] Technologies and equipment known to those skilled in the art may not be discussed in detail, but where appropriate, such technologies and equipment should be considered part of the specification.

[0040] In all the examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values.

[0041] It should be noted that similar labels and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be discussed further in subsequent figures.

[0042] This application provides a culture dish 1, which is suitable for cell culture under simulated microgravity on Earth.

[0043] Reference Figures 1-7 The culture dish 1 includes a dish body 11, a dish lid 12, and an annular sleeve portion 3. The dish lid 12 is placed on the dish body 11, and the annular sleeve portion 3 is disposed on the culture dish to form a seal between the dish body 11 and the dish lid 12.

[0044] The lid 12 has at least one vent hole 121, and a waterproof and breathable membrane 122 is provided on the lower surface of the lid 12, which covers the vent hole 121. The pore size of the waterproof and breathable membrane 122 is in the range of 0.2μm-0.5μm.

[0045] The upper surface of the lid 12 has an injection hole 123 that penetrates the lid 12. A plug 124 is provided inside the injection hole 123. The plug 124 can be inserted into the injection hole 123 or pulled out from the injection hole 123.

[0046] In this embodiment of the application, a petri dish 1 is provided, which mainly includes a dish body 11 and a dish lid 12. When the dish lid 12 is placed on the dish body 11, a seal is formed between the dish lid 12 and the dish body 11.

[0047] Specifically, the culture dish includes an annular sleeve 3, which is disposed on the culture dish and can apply a closing force to the lid 12 and the body 11 to ensure the airtightness of the culture dish 1. In this way, the culture dish is placed on the 3D rotating mechanism for ground simulation of microgravity cell culture, avoiding leakage of the culture dish.

[0048] Furthermore, a vent hole 121 is provided in the lid 12, and a waterproof and breathable membrane 122 covers the vent hole 121. The pore size of the waterproof and breathable membrane 122 is in the range of 0.2μm-0.5μm, which further ensures the airtightness of the culture dish 1. For example, the pore size range of the waterproof and breathable membrane 122 is 0.2μm, 0.25μm, 0.3μm, 0.4μm, 0.45μm, and 0.5μm.

[0049] In this embodiment, the pore size range of the waterproof and breathable membrane 122 of the cell culture dish is 0.2μm-0.5μm. This embodiment limits the pore size range of the waterproof and breathable membrane 122, ensuring that during 3D rotation culture, all components of the cell culture medium within the dish cannot permeate through the membrane while meeting the normal respiration and growth needs of the cells, thus preventing leakage.

[0050] In addition, some cell debris and particles are inevitably generated during cell culture. These debris and particles are usually quite large, generally above the micrometer level, far exceeding the pore size of the waterproof and breathable membrane. During cell culture, the cell debris and particles generated will not pass through the waterproof and breathable membrane 122, thus avoiding leakage problems in the culture dish.

[0051] In this embodiment, the pore size of the waterproof and breathable membrane 122 acts as a physical barrier. During cell culture, gas molecules smaller than the pore size of the waterproof and breathable membrane 122 can pass through it for free exchange, meeting the normal respiration and growth needs of the cells. Cell culture medium components larger than the waterproof and breathable membrane 122 and macromolecules generated during cell culture are directly blocked inside the culture dish. Therefore, apart from gas molecules, no other substances in the culture dish can pass through the waterproof and breathable membrane 122. The culture dish is placed on a 3D rotating mechanism for ground-based microgravity cell culture, preventing leakage.

[0052] When culture dish 1 is filled with culture medium and placed on a 3D rotating mechanism for microgravity-simulated cell culture on Earth, leakage is prevented because the lid 12 and the dish body 11 form a seal. Furthermore, a filling hole 123 is provided on the lid 12, allowing culture medium to be filled into the dish body 11 through the filling hole 123 when the lid 12 is on top of the dish body 11. Specifically, culture dish 1 includes a dish body 11 and a lid 12. When the lid 12 is on top of the dish body 11, a seal is formed between the lid 12 and the dish body 11, for example, by using a sealing gasket between the lid 12 and the dish body 11. Thus, when the culture dish is used for microgravity-simulated cell culture on Earth, and the dish 1 is placed on a 3D rotating mechanism, leakage of the culture medium inside the culture dish 1 is prevented due to the seal between the lid 12 and the dish body 11.

[0053] At least one vent hole 121 is provided on the dish lid 12. A waterproof and breathable membrane 122 is provided on the lower surface of the dish lid 12, covering the vent hole 121. Thus, when the dish lid 12 is placed over the dish body 11 and the stopper 124 is inserted into the injection hole 123, the culture medium inside the dish body 11 can exchange with external gases to meet the normal respiration and growth needs of cells during cell culture. The waterproof and breathable membrane 122 can be attached to the lower surface of the dish lid 12 by means of adhesion or other methods. The lower surface of the dish lid 12 is defined as the surface of the dish lid 12 facing inwards from the dish body 11 when it is placed over the dish body 11. The upper surface of the dish lid 12 is defined as the surface of the dish lid 12 facing away from the dish body 11 when it is placed over the dish body 11.

[0054] For example, in this embodiment, when the pore size of the waterproof and breathable membrane 122 is in the range of 0.2μm-0.5μm, the CO2 permeability of the waterproof and breathable membrane 122 is ≥10mL / (cm2·min·atm). The O2 permeability of the waterproof and breathable membrane 122 is ≥10mL / (cm2·min·atm), and the water vapor transmission rate (WVTR) is <100g / m2·24h, which meets the breathability requirements of the waterproof and breathable membrane 122.

[0055] With a pore size range of 0.2μm-0.5μm for the waterproof and breathable membrane 122, the hydrostatic pressure of the waterproof and breathable membrane 122 is ≥5kPa. The contact angle of the waterproof and breathable membrane is typically >110°, meeting the waterproof requirements of the waterproof and breathable membrane 122.

[0056] In addition, the waterproof and breathable membrane 122 is made of PTFE membrane, which is resistant to acids and alkalis (pH 6-8) and ethanol (wiping with 70% disinfectant). Even if the culture medium comes into contact with the membrane surface, the chemical inertness of the PTFE membrane can ensure that its structural integrity and breathability are not affected, fully meeting the routine requirements of the petri dish.

[0057] For example, when the pore size of the waterproof and breathable membrane 122 is in the range of 0.2μm-0.5μm, the thickness of the waterproof and breathable membrane is in the range of 20μm-50μm, which meets the waterproof and breathable requirements of the petri dish.

[0058] With the waterproof and breathable membrane 122 employing a PTFE microporous structure with a pore size range of 0.2μm-0.5μm and a membrane thickness designed to be 20μm-50μm, and with the waterproof and breathable membrane 122 satisfying both breathability and waterproofness, when applied in culture dishes, the service life of the waterproof and breathable membrane 122 can reach 4-8 weeks under conditions of rotation culture and culture medium contact with the membrane (in actual applications, the longest cell rotation culture cycle is 3-5 days, and the service life fully meets the requirements).

[0059] A liquid injection hole 123 is provided on the upper surface of the petri dish lid 12, and the liquid injection hole 123 penetrates the lid 12. When the liquid injection hole 123 is provided on the upper surface of the lid 12, the liquid injection hole 123 is provided in the thickness direction of the petri dish 1. When the lid 12 is placed on the petri dish body 11, it is convenient for the user to fill the petri dish with culture medium through the liquid injection hole 123.

[0060] With the stopper 124 removed from the injection hole 123, filling the culture dish 1 with culture medium through the injection hole 123 ensures that there are no air bubbles in the culture dish 1. Furthermore, injecting or drawing culture medium into or from the culture dish 1 through the injection hole 123 avoids opening the lid 12 to inject or draw culture medium from the dish body 11, thus preventing contamination of the culture medium in the dish body 11 by the external environment.

[0061] Therefore, in this embodiment, a culture dish 1 is provided. When the dish lid 12 is placed on the dish body 11, the dish body 11 and the dish lid 12 are fastened together by the annular sleeve 3. Furthermore, by limiting the pore size of the waterproof and breathable membrane 122, a seal is formed between the dish body 11 and the dish lid 12. The culture dish is placed on a 3D rotating mechanism for ground-based microgravity-simulated cell culture, preventing leakage. In addition, when the culture dish 1 is filled with culture medium through the injection hole 123, the vent hole 121, in conjunction with the waterproof and breathable membrane 122, allows for gas exchange between the culture medium and the external environment, meeting the culture requirements.

[0062] In one embodiment, refer to Figure 1 , Figure 4 , Figure 7 and Figure 8 The lid 12 has two vent holes 121 and two waterproof and breathable membranes 122 are provided on the lower surface of the lid 12. One waterproof and breathable membrane 122 corresponds to one vent hole 121 and the other waterproof and breathable membrane 122 corresponds to the other vent hole 121.

[0063] In this embodiment, two vent holes 121 are provided on the lid 12. The diameters of the two vent holes 121 can be the same or different. When two vent holes 121 are provided on the lid 12, two waterproof and breathable membranes 122 are provided on the lower surface of the lid 12. One waterproof and breathable membrane 122 covers the other waterproof and breathable membrane 122, and the other waterproof and breathable membrane 122 covers the other vent hole 121.

[0064] Two vent holes 121 are made on the lid 12 of the culture dish to ensure that the culture medium in the culture dish 1 is not contaminated, so as to meet the normal respiration and growth needs of the cells during cell culture.

[0065] Optionally, if two vent holes 121 are provided on the lid 12, a waterproof and breathable membrane 122 can also be provided on the lower surface of the lid 12, which covers both vent holes 121 at the same time.

[0066] In one embodiment, refer to Figure 1 , Figure 2 and Figure 4 The lid 12 has a lid body 125 and a sidewall 126 extending along the outer periphery of the lid body 125; when the lid 12 covers the dish body 11, there is a gap between the lower surface of the sidewall 126 and the outer surface of the dish body 11.

[0067] In this embodiment, the lid 12 includes a lid body 125 and a sidewall 126. The lid body 125 can be disc-shaped, and the sidewall 126 is located on the outer periphery of the lid body 125. For example, when the lid 12 is placed on the dish body 11, the sidewall 126 extends toward the dish body 11. The lid body 125 and the sidewall 126 can be an integrally formed structural component.

[0068] The petri dish 1 has a cylindrical body 11 with an open end face. When the lid 12 is placed on the petri dish 11, the open end face of the petri dish 11 seals with the lower surface of the lid 12, and there is a gap between the lower surface of the side wall 126 of the lid 12 and the petri dish 11. This provides a certain amount of space for movement between the lid 12 and the petri dish 11, allowing the lid 12 to be placed on the petri dish 11 very easily. For example, the inner diameter of the side wall of the lid 12 can be slightly larger than the outer diameter of the petri dish 11 for ease of operation.

[0069] In one embodiment, a sealing gasket 1251 is provided on the lower surface of the lid body 125. The sealing gasket 1251 needs to be a medical-grade polytetrafluoroethylene (PTFE) sealing gasket.

[0070] In this embodiment, a sealing gasket 1251 is provided on the lower surface of the lid body 125. When the lid 12 covers the body 11, the sealing gasket 1251 is located between the lid body 125 and the open end face of the body 11, ensuring the sealing between the lid 12 and the body 11.

[0071] In one embodiment, refer to Figure 2 and Figure 4 The lid 12 includes a lid body 125, on which a boss portion 127 is provided, and the liquid injection hole 123 penetrates the boss portion 127 and the lid body 125.

[0072] In this embodiment, the lid 12 includes a lid body 125, which may be a disc-shaped structure. A boss 127 is provided on the lid body 125. For example, the outer surface of the lid body 125 includes a first region and a second region. The boss 127 is provided in the second region of the outer surface of the lid body 125, while no boss 127 is provided in the first region. Optionally, the lid body 125 and the boss 127 may be integrally formed.

[0073] The injection hole 123 penetrates the boss portion 127 and the plate cover body 125. Thus, the axial dimension of the injection hole 123 is greater than the thickness dimension of the plate cover body 125. It can also be understood that the injection hole 123 protrudes more than the plate cover body 125, making it easier for users to inject culture medium into the plate body 11 through the injection hole 123 or to draw culture medium from the plate body 11 through the injection hole 123.

[0074] In cases where the injection hole 123 protrudes more than the cap body 125, a stopper 124 is installed inside the injection hole 123. The stopper 124 also protrudes more than the cap body 125, which makes it convenient for the user to pull the stopper 124 out of the injection hole 123 or to insert the stopper 124 into the injection hole 123.

[0075] In one embodiment, refer to Figure 1 , Figure 2 , Figure 4 and Figure 6 A sealing ring 1241 is provided on the outer peripheral wall of the plug 124; when the plug 124 is inserted into the injection hole 123, the sealing ring 1241 abuts against the inner peripheral wall of the injection hole 123. The sealing ring 1241 needs to be a medical-grade polytetrafluoroethylene (PTFE) sealing ring.

[0076] In this embodiment, a sealing ring 1241 is provided on the outer peripheral wall of the stopper 124. For example, the sealing ring 1241 is sleeved on the outer peripheral wall of the stopper 124, or a recessed groove is provided on the outer peripheral wall of the stopper 124, with the sealing ring 1241 sleeved on the outer peripheral wall of the stopper 124 and embedded in the recessed groove. This can improve the firmness of the sealing ring 1241 on the stopper 124. Thus, when the stopper 124 is placed in the injection hole 123, the sealing performance between the stopper 124 and the injection hole 123 can also be improved.

[0077] When the stopper 124 is inserted into the injection hole 123, the sealing ring 1241 abuts against the inner peripheral wall of the injection hole 123, which further ensures the sealing between the lid 12 and the body 11.

[0078] In one embodiment, refer to Figures 7-9 The annular sleeve portion 3 includes a first sleeve portion 31 and a second sleeve portion 32; the first sleeve portion 31 is provided with a first protrusion 311 in the circumferential direction; the second sleeve portion 32 is provided with a second protrusion 321 in the circumferential direction.

[0079] When the first sleeve portion 31 is sleeved on the lid 12 and the second sleeve portion 32 is sleeved on the body 11, the first protrusion 311 and the second protrusion 321 are fastened together.

[0080] In this embodiment, the annular sleeve portion 3 includes a first sleeve portion 31 and a second sleeve portion 32. The first sleeve portion 31 is sleeved on the lid 12, and the second sleeve portion 32 is sleeved on the body 11. The first protrusion 311 on the first sleeve portion 31 and the second protrusion 321 on the second sleeve portion 32 are arranged opposite to each other. The first protrusion 311 and the second protrusion 321 can be fastened together by a fastening sleeve or other fastening components. When the first protrusion 311 and the second protrusion 321 are fastened together, the annular sleeve portion applies a fastening force to the lid 12 and the body 11, ensuring the sealing between the lid 12 and the body 11.

[0081] In one embodiment, refer to Figures 7-9 The culture dish further includes a fastener 2. The first protrusion 311 has a first threaded hole, and the second protrusion 321 has a second threaded hole. The fastener 2 passes through the first threaded hole and the second threaded hole to fasten the first protrusion 311 and the second protrusion 321. For example, the fastener 2 can be a bolt or the like.

[0082] In this embodiment, when the lid 12 covers the body 11, the first fitting portion 31 is fitted onto the lid 12, and the second fitting portion 32 is fitted onto the body 11. The first protrusion 311 and the second protrusion 321 are correspondingly arranged. The fastener 2 passes through the first threaded hole and the second threaded hole in sequence to fasten the first protrusion 311 and the second protrusion 321 together. With the fastener 2 fastening the first protrusion 311 and the second protrusion 321 together, the annular fitting portion 3 also applies a tightening force to the lid 12 and the body 11, ensuring the sealing of the lid 12 and the body 11.

[0083] The above embodiments mainly describe the differences between the various embodiments. As long as the different optimization features between the various embodiments are not contradictory, they can be combined to form a better embodiment. For the sake of brevity, they will not be elaborated here.

[0084] While specific embodiments of this application have been described in detail by way of examples, those skilled in the art should understand that the above examples are for illustrative purposes only and are not intended to limit the scope of this application. Those skilled in the art should understand that modifications can be made to the above embodiments without departing from the scope and spirit of this application. The scope of this application is defined by the appended claims.

Claims

1. A petri dish, characterized in that, The culture dish includes: a dish body (11), a dish lid (12), and an annular sleeve part (3). The dish lid (12) is placed on the dish body (11), and the annular sleeve part (3) is disposed on the culture dish so that the dish body (11) and the dish lid (12) form a seal. The lid (12) of the dish is provided with at least one vent hole (121), and a waterproof and breathable membrane (122) is provided on the lower surface of the lid (12). The waterproof and breathable membrane (122) covers the vent hole (121), and the pore size of the waterproof and breathable membrane (122) is in the range of 0.2μm-0.5μm. The upper surface of the lid (12) is provided with a liquid injection hole (123), which penetrates the lid (12). A plug (124) is provided inside the liquid injection hole (123). The plug (124) can be inserted into the liquid injection hole (123) or pulled out from the liquid injection hole (123).

2. The petri dish according to claim 1, characterized in that, The lid (12) has two vent holes (121) and two waterproof and breathable membranes (122) are provided on the lower surface of the lid (12). One waterproof and breathable membrane (122) corresponds to one vent hole (121) and the other waterproof and breathable membrane (122) corresponds to the other vent hole (121).

3. The petri dish according to claim 1, characterized in that, The lid (12) has a lid body (125) and a sidewall (126) extending along the outer periphery of the lid body (125); When the lid (12) is placed on the body (11), there is a gap between the lower surface of the side wall (126) and the outer surface of the body (11).

4. The petri dish according to claim 3, characterized in that, A sealing gasket (1251) is provided on the lower surface of the lid body (125).

5. The petri dish according to claim 1, characterized in that, The lid (12) includes a lid body (125), on which a boss (127) is provided, and the injection hole (123) penetrates the boss (127) and the lid body (125).

6. The petri dish according to claim 1 or 5, characterized in that, A sealing ring (1241) is provided on the outer peripheral wall of the plug (124); When the plug (124) is inserted into the injection hole (123), the sealing ring (1241) abuts against the inner peripheral wall of the injection hole (123).

7. The petri dish according to claim 1, characterized in that, The annular sleeve portion (3) includes a first sleeve portion (31) and a second sleeve portion (32); The first sleeve (31) is provided with a first protrusion (311) in the circumferential direction; The second set of parts (32) is provided with a second protrusion (321) in the circumferential direction; When the first sleeve portion (31) is sleeved on the lid (12) and the second sleeve portion (32) is sleeved on the body (11), the first protrusion (311) and the second protrusion (321) are fastened together.

8. The petri dish according to claim 7, characterized in that, The culture dish also includes a fastener (2), on which a first threaded hole is provided on the first protrusion (311) and a second threaded hole is provided on the second protrusion (321). The fastener (2) passes through the first threaded hole and the second threaded hole to fasten the first protrusion (311) and the second protrusion (321).

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