An observable multi-layer cell culture plate

By combining an L-shaped culture plate with an angle adjustment component, the problem of traditional culture plates being unable to directly observe the three-dimensional growth of cells was solved. This enabled multi-angle adjustment and multi-layer splicing of the culture plate, improving the convenience and stability of cell observation.

CN224337581UActive Publication Date: 2026-06-09LANZHOU UNIVERSITY OF TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
LANZHOU UNIVERSITY OF TECHNOLOGY
Filing Date
2025-07-04
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing cell culture plates are designed as flat single-layer plates, making it difficult to intuitively observe the growth status of cells in three-dimensional space by adjusting the angle. In particular, the operation is cumbersome and may affect cell viability when observing cell migration and differentiation processes from multiple angles.

Method used

An observable multilayer cell culture plate was designed, which uses an L-shaped culture plate combined with an angle adjustment component and a limiting component. The culture plate can be tilted at any angle by means of an arc-shaped guide plate and a rotating block. The angle is locked by a reset spring and a limiting spring, and the culture plate can be quickly assembled and disassembled.

Benefits of technology

It enables arbitrary angle adjustment and multi-layer splicing of culture plates, meeting the needs of cell observation under different tilt angles of the microscope, simplifying the operation process, and avoiding the impact of culture medium shaking on cell viability.

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Abstract

The utility model relates to the field of culture plate discloses a kind of observable multilayer cell culture plate, including L-shaped culture plate, the left side outer wall of L-shaped culture plate is provided with angle adjusting assembly, the inner side side wall of L-shaped culture plate is equipped with sliding slot, the inner wall sliding connection of L-shaped culture plate sliding slot has T block, the outer side side wall of T block is fixedly connected with straight culture plate, the inner side side wall of straight culture plate is provided with limiting component, the center of fixed frame is fixedly connected with arc guide plate, the sidewall fixed connection of L-shaped culture plate bottom end has protruding block, the inner side side wall of protruding block is elastically connected with press block by reset spring.The utility model in the cooperation of arc guide plate and rotating block can realize the arbitrary angle inclination of L-shaped culture plate, the automatic locking function of press block and vertical groove prevents shaking after angle adjustment, satisfies the cell dynamic observation demand under different inclination viewing angle of microscope.
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Description

Technical Field

[0001] This invention relates to the field of culture plates, and more particularly to an observable multilayer cell culture plate. Background Technology

[0002] A cell culture plate is a laboratory supply used for cell culture, consisting of a round or square culture medium base made of plastic and a sealable lid.

[0003] However, most existing cell culture plates are flat single-layer designs. During observation, the culture plate must be placed horizontally on the microscope stage, and the staff can only observe from the vertical direction. It is difficult to fully observe the growth status of cells in three-dimensional space. Especially for experiments that require observation of dynamic processes such as cell migration and differentiation from multiple angles, traditional culture plates cannot achieve intuitive observation by adjusting the angle. It is often necessary to use the tilting stage of the microscope or repeatedly move the culture plate. This is not only cumbersome, but may also cause the culture medium to shake due to frequent movement, affecting cell viability. Therefore, an observable multi-layer cell culture plate is proposed to solve the above problems. Utility Model Content

[0004] To overcome the above shortcomings, this invention provides an observable multilayer cell culture plate, which aims to improve the problem that traditional culture plates in the prior art cannot be directly observed by adjusting the angle, and often require the use of a microscope's tilting stage or repeated movement of the culture plate.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: an observable multilayer cell culture plate, comprising an L-shaped culture plate, wherein an angle adjustment component is provided on the left outer wall of the L-shaped culture plate, a sliding groove is provided on the inner side wall of the L-shaped culture plate, a T-shaped block is slidably connected to the inner wall of the sliding groove of the L-shaped culture plate, a straight culture plate is fixedly connected to the outer side wall of the T-shaped block, and a limit component is provided on the inner side wall of the straight culture plate;

[0006] The angle adjustment assembly includes a fixed frame, an arc-shaped guide plate fixedly connected to the center of the fixed frame, a rotating block rotatably connected to the inner wall of the top of the fixed frame, a protrusion fixedly connected to the side wall of the bottom of the L-shaped culture plate, and a push block elastically connected to the inner side wall of the protrusion through a return spring.

[0007] As a further description of the above technical solution:

[0008] The limiting component includes an insert block, which is elastically connected to the inner sidewall of the straight culture plate by a limiting spring.

[0009] As a further description of the above technical solution:

[0010] The surface of the arc-shaped guide plate is provided with a through arc-shaped groove, and the protrusion is slidably connected to the inner wall of the arc-shaped groove of the arc-shaped guide plate through it.

[0011] As a further description of the above technical solution:

[0012] One end of the reset spring is fixedly connected to the inner side wall of the protrusion, and the other end of the reset spring is fixedly connected to the outer side wall of the button.

[0013] As a further description of the above technical solution:

[0014] The surface of the arc-shaped guide plate has a vertical groove, and the outer wall of the block is in contact with the inner wall of the vertical groove.

[0015] As a further description of the above technical solution:

[0016] The rotating block is fixedly connected to the side wall at the top of the L-shaped culture plate, and the pressing block is slidably connected to the inner wall of the protrusion.

[0017] As a further description of the above technical solution:

[0018] One end of the limiting spring is fixedly connected to the outer sidewall of the insertion block, and the other end of the limiting spring is fixedly connected to the inner sidewall of the straight culture plate.

[0019] As a further description of the above technical solution:

[0020] The insert is slidably connected to the inner sidewall of the straight culture plate. The inner surface of the L-shaped culture plate has a slot, and the outer wall of the insert is inserted into the inner wall of the slot.

[0021] This utility model has the following beneficial effects:

[0022] 1. In this utility model, the combination of the arc-shaped guide plate and the rotating block can realize the tilting of the L-shaped culture plate at any angle. The automatic locking function of the block and the vertical groove prevents shaking after the angle is adjusted, thus meeting the needs of dynamic cell observation under different tilt angles of the microscope.

[0023] 2. In this utility model, the elastic insert and limiting spring design of the limiting component enable the straight culture plate to be quickly assembled and disassembled with one hand when stacked, effectively avoiding the risk of tipping over in traditional stacking methods. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall three-dimensional structure of an observable multilayer cell culture plate proposed in this utility model.

[0025] Figure 2This is a schematic diagram of a partial cross-sectional view of the protrusions in an observable multilayer cell culture plate proposed in this utility model.

[0026] Figure 3 This invention proposes an observable multilayer cell culture plate. Figure 2 Enlarged structural diagram of section A;

[0027] Figure 4 This is a partial cross-sectional view of a straight culture plate of an observable multilayer cell culture plate proposed in this utility model.

[0028] Legend:

[0029] 1. L-shaped culture plate; 2. Angle adjustment assembly; 21. Fixing frame; 22. Arc-shaped guide plate; 23. Rotating block; 24. Protrusion; 25. Reset spring; 26. Press block; 3. Straight culture plate; 4. Limiting assembly; 41. Insertion block; 42. Limiting spring; 5. T-shaped block. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] Reference Figures 1-3 This utility model provides an embodiment of an observable multilayer cell culture plate, including an L-shaped culture plate 1. The L-shaped culture plate 1 is L-shaped in general, and a culture groove is also provided at its bottom end. An angle adjustment component 2 is provided on the left outer wall of the L-shaped culture plate 1. A sliding groove is provided on the inner side wall of the L-shaped culture plate 1. A T-shaped block 5 is slidably connected to the inner wall of the sliding groove of the L-shaped culture plate 1. A straight culture plate 3 is fixedly connected to the outer side wall of the T-shaped block 5. The T-shaped block 5 can move to multiple positions on the inner wall of the L-shaped culture plate 1, thereby adjusting the height of the straight culture plate 3, which facilitates observation by the staff. Furthermore, by moving the T-shaped block 5, multiple sets of straight culture plates 3 can be vertically spliced. A limit component 4 is provided on the inner side wall of the straight culture plate 3.

[0032] Reference Figure 2 and Figure 3The angle adjustment component 2 includes a fixed frame 21, with an arc-shaped guide plate 22 fixedly connected to the center of the fixed frame 21. A rotating block 23 is rotatably connected to the inner wall of the top of the fixed frame 21. The rotating block 23 is fixedly connected to the side wall of the top of the L-shaped culture plate 1. A protrusion 24 is fixedly connected to the side wall of the bottom of the L-shaped culture plate 1. The surface of the arc-shaped guide plate 22 has a through arc-shaped groove. The arc-shaped groove of the arc-shaped guide plate 22 is opened with the rotating block 23 as the rotation center point. When the protrusion 24 slides on the inner wall of the arc-shaped groove of the arc-shaped guide plate 22, it will drive the rotating block 23 to rotate around the inner wall of the top of the fixed frame 21, thereby adjusting the overall tilt angle of the L-shaped culture plate 1, which facilitates the operation of the equipment. Observation shows that the protrusion 24 is slidably connected to the inner wall of the arc-shaped groove of the arc-shaped guide plate 22. The inner side wall of the protrusion 24 is elastically connected to the button 26 through the return spring 25. One end of the return spring 25 is fixedly connected to the inner side wall of the protrusion 24, and the other end of the return spring 25 is fixedly connected to the outer side wall of the button 26. The function of the return spring 25 is to automatically reset the position of the button 26 after it has been pressed and moved. The surface of the arc-shaped groove of the arc-shaped guide plate 22 has a vertical groove. The outer wall of the button 26 contacts the inner wall of the vertical groove. The insertion between the two can fix the protrusion 24 and the L-shaped culture plate 1 in multiple positions after they have been rotated. The button 26 is slidably connected to the inner wall of the protrusion 24.

[0033] Reference Figure 1 and Figure 4 The limiting component 4 includes an insert block 41, which is elastically connected to the inner side wall of the straight culture plate 3 via a limiting spring 42. One end of the limiting spring 42 is fixedly connected to the outer side wall of the insert block 41, and the other end is fixedly connected to the inner side wall of the straight culture plate 3. The function of the limiting spring 42 is to automatically reset the position of the insert block 41 after it has been moved. The insert block 41 passes through and is slidably connected to the inner side wall of the straight culture plate 3. A slot is provided on the inner surface of the L-shaped culture plate 1. The outer wall of the insert block 41 is inserted into the inner wall of the slot. The insertion between the two can fix the straight culture plate 3 in multiple positions that slide inside the L-shaped culture plate 1, and also facilitates the stacking and splicing of multiple sets of straight culture plates 3.

[0034] Working principle: The angle adjustment component 2 has an arc-shaped guide plate 22 fixedly connected to the center of the fixed frame 21, and a rotating block 23 rotatably connected to the inner wall of the top. The rotating block 23 is fixedly connected to the top side wall of the L-shaped culture plate 1. The protrusion 24 on the bottom side wall of the L-shaped culture plate 1 passes through and slides in the arc groove of the arc-shaped guide plate 22. Since the arc groove of the arc-shaped guide plate 22 is opened with the rotating block 23 as the rotation center, when the L-shaped culture plate 1 is pushed, the protrusion 24 slides in the arc groove, driving the rotating block 23 to rotate around the top inner wall of the fixed frame 21, thereby realizing the adjustment of the overall tilt angle of the L-shaped culture plate 1.

[0035] After adjusting to the appropriate angle, press the button 26 on the inner side wall of the protrusion 24. The button 26 slides against the elastic force of the reset spring 25 on the inner wall of the protrusion 24. When the outer wall of the button 26 is aligned with the vertical groove on the surface of the arc-shaped guide plate 22, the reset spring 25 pushes the button 26 to insert into the vertical groove. By inserting the button 26 into the vertical groove, the protrusion 24 is fixed, thereby locking the current tilt angle of the L-shaped culture plate 1, which makes it convenient for staff to observe the cell culture from different perspectives.

[0036] The inner sidewall of the L-shaped culture plate 1 has a sliding groove that slides into the T-shaped block 5. The outer sidewall of the T-shaped block 5 is fixedly connected to the straight culture plate 3. When the height of the straight culture plate 3 needs to be adjusted, the T-shaped block 5 is moved along the inner wall of the L-shaped culture plate 1. The T-shaped block 5 can move the straight culture plate 3 to multiple positions to meet different observation needs. The insertion block 41 on the inner sidewall of the straight culture plate 3 is elastically connected by a limiting spring 42. When the straight culture plate 3 is slid to a suitable position, the insertion block 41 is in contact with the limiting spring 42. Under the action of the mechanism, the straight culture plate 3 pops out and is inserted into the slot on the inner surface of the L-shaped culture plate 1. By inserting the insert block 41 into the slot, the straight culture plate 3 is fixed in the current position. If multi-layer splicing is required, the T-shaped block 5 of another set of straight culture plates 3 is inserted into the corresponding position on the inner side of the fixed straight culture plate 3. Similarly, by inserting the insert block 41 into the slot and the elastic force of the limiting spring 42, multiple sets of straight culture plates 3 can be vertically stacked and spliced, increasing the number of culture plate layers and improving space utilization.

[0037] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An observable multi-layer cell culture plate comprising an L-shaped culture plate (1), characterized in that: An angle adjustment component (2) is provided on the left outer wall of the L-shaped culture plate (1), a sliding groove is provided on the inner side wall of the L-shaped culture plate (1), a T-shaped block (5) is slidably connected to the inner wall of the sliding groove of the L-shaped culture plate (1), a straight culture plate (3) is fixedly connected to the outer side wall of the T-shaped block (5), and a limit component (4) is provided on the inner side wall of the straight culture plate (3). The angle adjustment assembly (2) includes a fixed frame (21), an arc-shaped guide plate (22) is fixedly connected to the center of the fixed frame (21), a rotating block (23) is rotatably connected to the inner wall of the top of the fixed frame (21), the rotating block (23) is fixedly connected to the side wall of the top of the L-shaped culture plate (1), a protrusion (24) is fixedly connected to the side wall of the bottom of the L-shaped culture plate (1), and a pressing block (26) is elastically connected to the inner side wall of the protrusion (24) through a return spring (25).

2. The observable multilayer cell culture plate according to claim 1, characterized in that: The limiting component (4) includes an insert (41), which is elastically connected to the inner sidewall of the straight culture plate (3) by a limiting spring (42).

3. The observable multilayer cell culture plate according to claim 1, characterized in that: The surface of the arc-shaped guide plate (22) is provided with a through arc-shaped groove, and the protrusion (24) slides through it and is connected to the inner wall of the arc-shaped groove of the arc-shaped guide plate (22).

4. The observable multilayer cell culture plate according to claim 1, characterized in that: One end of the reset spring (25) is fixedly connected to the inner sidewall of the protrusion (24), and the other end of the reset spring (25) is fixedly connected to the outer sidewall of the button (26).

5. The observable multilayer cell culture plate according to claim 1, characterized in that: The surface of the arc-shaped guide plate (22) has a vertical groove, and the outer wall of the push block (26) is in contact with the inner wall of the vertical groove.

6. The observable multilayer cell culture plate according to claim 1, characterized in that: The push block (26) is slidably connected to the inner wall of the protrusion (24).

7. The observable multilayer cell culture plate according to claim 2, characterized in that: One end of the limiting spring (42) is fixedly connected to the outer sidewall of the insert (41), and the other end of the limiting spring (42) is fixedly connected to the inner sidewall of the straight culture plate (3).

8. The observable multilayer cell culture plate according to claim 2, characterized in that: The insert (41) is slidably connected to the inner side wall of the straight culture plate (3). The inner surface of the L-shaped culture plate (1) is provided with a slot. The outer wall of the insert (41) is inserted into the inner wall of the slot.