Gas source switching structure for carbon dioxide incubator

Abstract

This utility model discloses a gas source switching structure for a carbon dioxide incubator, including an incubator body with a sealed door. A fixing plate is bolted to the bottom of one outer wall of the incubator body, and the top outer wall of the fixing plate has equidistantly distributed positioning grooves. A support ring is provided above the fixing plate, and a carbon dioxide storage bottle, a spare carbon dioxide bottle, a first gas storage bottle, and a second gas storage bottle are respectively inserted and installed on the inner wall of the support ring. This utility model uses a spare gas bottle to connect the same gas source supply link, so that when the main gas bottle is insufficient and needs to be replaced, the gas source can be continuously supplied without interruption, making the cell culture environment more realistic. The carbon dioxide can be controlled by a solenoid valve, and a pressure reducing valve is used to reduce the pressure during the control process to ensure the safety of the gas supply link. It can also be used in conjunction with a pressure sensor for real-time feedback processing.

Description

Technical Field

[0001] This utility model relates to the field of carbon dioxide incubator technology, and in particular to a gas source switching structure for a carbon dioxide incubator. Background Technology

[0002] A carbon dioxide incubator is a device that simulates the growth environment of cells or tissues inside a living organism. It requires stable temperature, stable CO2 levels, constant pH, and high relative humidity for in vitro cell / tissue culture. It is a crucial piece of equipment for immunology, oncology, genetics, and bioengineering. However, to ensure a sufficient gas supply to the cell / tissue culture chamber during the culture process, an external gas supply tank is often needed. But using a gas supply tank to supply gas to a carbon dioxide incubator presents some problems:

[0003] 1. Existing incubators can only supply carbon dioxide gas, while cell and tissue culture requires a constant pH environment, low-oxygen culture, anaerobic culture, etc., which requires the addition or adjustment of other gases. However, existing incubators cannot effectively add these gases, resulting in defects in the culture environment.

[0004] 2. When supplying carbon dioxide gas to the existing incubator, if the gas in the bottle is insufficient and needs to be replaced, the supply can only be temporarily paused to replace the bottle in order to resume the supply of carbon dioxide. However, the temporary interruption of the gas supply will also have a certain impact on the cell culture environment, thereby affecting the culture effect. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a gas source switching structure for a carbon dioxide incubator.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A gas source switching structure for a carbon dioxide incubator includes an incubator body with a sealed door. A fixed support plate is bolted to the bottom of one outer wall of the incubator body, and the top outer wall of the fixed support plate has equidistantly distributed positioning grooves. A support ring is provided above the fixed support plate, and a carbon dioxide storage bottle, a carbon dioxide spare bottle, a first gas storage bottle, and a second gas storage bottle are respectively inserted and installed on the inner wall of the support ring. A first pressure reducing valve is screwed to the port of both the carbon dioxide storage bottle and the carbon dioxide spare bottle, and a first connecting pipe is screwed to one end of the first pressure reducing valve. A first pressure sensor is installed in the middle of the first connecting pipe, and a first solenoid valve is screwed to the end of the first connecting pipe away from the first pressure reducing valve. A first T-shaped tee pipe is connected between the two first solenoid valves, and a first air inlet pipe is sleeved on the other end of the first T-shaped tee pipe. A second pressure reducing valve is screwed to the port of both the first gas storage bottle and the second gas storage bottle, and a second connecting pipe is screwed to one end of the second pressure reducing valve.

[0008] A second pressure sensor is installed in the middle of the second connecting pipe, and a second solenoid valve is screwed to the end of the second connecting pipe away from the second pressure reducing valve. A second T-shaped tee pipe is connected between the two second solenoid valves, and a second air inlet pipe is sleeved on the other end of the second T-shaped tee pipe. An air supply pipe is connected to the inner wall of one side of the bottom of the incubator. A gas mixing component is connected between the first air inlet pipe and the second air inlet pipe. The first pressure sensor, the first solenoid valve, the second pressure sensor, and the second solenoid valve are all connected to a PLC controller through signal lines, and the PLC controller is connected to an external power supply through wires.

[0009] As a further improvement of this utility model: the inner wall of each of the supporting collars is bonded with a protective sponge ring, and the inner wall dimensions of the protective sponge ring are adapted to the outer wall dimensions of the carbon dioxide storage bottle, the spare carbon dioxide bottle, the first gas storage bottle, and the second gas storage bottle.

[0010] As a further embodiment of this utility model: the air mixing component includes a mixing shell that is interconnected with the first air intake pipe and the second air intake pipe, and an air mixing cavity is provided at the axial center of the mixing shell, and arc-shaped through grooves are provided on both inner walls of the air mixing cavity.

[0011] As a further improvement of this utility model: both sides of the inner wall of the arc-shaped through groove are provided with slots in the vertical direction, and an arc-shaped airflow guide plate is inserted and installed on the inner wall of the slot.

[0012] As a further embodiment of this utility model: a sealing cover plate is screwed to the top of the hybrid shell, and an L-shaped exhaust pipe is connected to the top axis of the sealing cover plate. The end of the L-shaped exhaust pipe away from the sealing cover plate is sleeved on the end of the gas transmission pipe.

[0013] As a further embodiment of this utility model: the first pressure sensor is installed 10-20cm downstream of the first pressure reducing valve, and the second pressure sensor is installed 10-20cm downstream of the second pressure reducing valve.

[0014] As a further improvement of this utility model: both the coil circuits of the first solenoid valve and the second solenoid valve need to be connected in parallel with an RC absorption circuit, and the RC absorption circuit includes a 100Ω resistor and a 0.1μF capacitor to prevent the back electromotive force from damaging the PLC controller when the power is off.

[0015] Compared with the prior art, this utility model provides a gas source switching structure for a carbon dioxide incubator, which has the following beneficial effects:

[0016] The carbon dioxide incubator designed in this way uses a gas source switching structure. It uses a spare gas cylinder to connect the same gas source supply chain, so that when the main gas cylinder is insufficient and needs to be replaced, the gas source can be continuously supplied without interruption, making the cell culture environment more realistic. The carbon dioxide can be controlled by a solenoid valve, and a pressure reducing valve is used to reduce the pressure during the control process to ensure the safety of the gas supply chain. It can also be used in conjunction with a pressure sensor for real-time feedback processing.

[0017] The carbon dioxide incubator designed in this way uses a gas source switching structure. When multiple gas sources are supplied simultaneously and continuously, the mixed gases are introduced into the mixing shell and uniformly dispersed by an arc-shaped airflow guide plate. This allows for better gas mixing and more uniform gas dispersion after delivery to the incubator, resulting in a more realistic simulated environment.

[0018] The parts of the device not covered herein are the same as or can be implemented using existing technologies. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of a gas source switching structure for a carbon dioxide incubator proposed in this utility model.

[0020] Figure 2 This is a side view of the overall structure of a gas source switching structure for a carbon dioxide incubator proposed in this utility model;

[0021] Figure 3 This is a first-view structural schematic diagram of a gas source switching structure for a carbon dioxide incubator proposed in this utility model.

[0022] Figure 4 This is a partial structural diagram of a gas source switching structure for a carbon dioxide incubator proposed in this utility model.

[0023] In the diagram: 1. Incubator body; 2. Sealed door; 3. Fixed support plate; 4. Positioning groove; 5. Support collar; 6. Protective sponge ring; 7. Carbon dioxide storage bottle; 8. Carbon dioxide spare bottle; 9. First gas storage bottle; 10. Second gas storage bottle; 11. First pressure reducing valve; 12. First connecting pipe; 13. First pressure sensor; 14. First solenoid valve; 15. First T-shaped tee pipe; 16. First air inlet pipe; 17. Second pressure reducing valve; 18. Second connecting pipe; 19. Second pressure sensor; 20. Second solenoid valve; 21. Second T-shaped tee pipe; 22. Second air inlet pipe; 23. Mixing shell; 24. Mixing cavity; 25. Arc-shaped through groove; 26. Arc-shaped air guide plate; 27. Sealing cover plate; 28. L-shaped exhaust pipe; 29. ​​Gas delivery pipe. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Example 1:

[0025] A gas source switching structure for a carbon dioxide incubator, as described in this embodiment... Figure 1-4 As shown, the incubator includes an incubator body 1 with a sealing door 2. A fixing plate 3 is bolted to the bottom of one outer wall of the incubator body 1. The top outer wall of the fixing plate 3 has evenly spaced positioning grooves 4. A support ring 5 is positioned above the fixing plate 3. A carbon dioxide storage bottle 7, a spare carbon dioxide bottle 8, a first gas storage bottle 9, and a second gas storage bottle 10 are respectively inserted and installed into the inner wall of the support ring 5. A first pressure reducing valve 11 is screwed to the ports of both the carbon dioxide storage bottle 7 and the spare carbon dioxide bottle 8. One end of the first gas storage bottle 1 is screwed and fixed with a first connecting pipe 12. A first pressure sensor 13 is installed in the middle of the first connecting pipe 12. A first solenoid valve 14 is screwed and fixed at the end of the first connecting pipe 12 away from the first pressure reducing valve 11. A first T-shaped three-way pipe 15 is connected between the two first solenoid valves 14. A first air inlet pipe 16 is sleeved at the other end of the first T-shaped three-way pipe 15. A second pressure reducing valve 17 is screwed and fixed at the ports of the first gas storage bottle 9 and the second gas storage bottle 10. A second connecting pipe 18 is screwed and fixed at one end of the second pressure reducing valve 17.

[0026] A second pressure sensor 19 is installed in the middle of the second connecting pipe 18, and a second solenoid valve 20 is screwed to the end of the second connecting pipe 18 away from the second pressure reducing valve 17. A second T-shaped three-way pipe 21 is connected between the two second solenoid valves 20, and a second air inlet pipe 22 is sleeved on the other end of the second T-shaped three-way pipe 21. An air supply pipe 29 is connected to the inner wall of one side of the bottom of the incubator 1. A gas mixing component is connected between the first air inlet pipe 16 and the second air inlet pipe 22. The first pressure sensor 13, the first solenoid valve 14, the second pressure sensor 19 and the second solenoid valve 20 are all connected to a PLC controller through signal lines. The PLC controller is connected to an external power supply through wires. The PLC controller can be a Siemens S7-200 SMART series.

[0027] By using backup gas cylinders to connect the same gas supply chain, the gas supply can be continuously supplied without interruption when the main gas cylinder is insufficient and needs to be replaced, thus making the cell culture environment more realistic. The carbon dioxide can be controlled by a solenoid valve, and pressure is reduced by a pressure reducing valve during the control process to ensure the safety of the gas supply chain. It can also be used in conjunction with a pressure sensor for real-time feedback processing.

[0028] The inner wall of the support collar 5 is bonded with a protective sponge ring 6, and the inner wall size of the protective sponge ring 6 is adapted to the outer wall size of the carbon dioxide storage bottle 7, the carbon dioxide spare bottle 8, the first gas storage bottle 9, and the second gas storage bottle 10. The gas mixing component includes a mixing shell 23 that is connected to the first air inlet pipe 16 and the second air inlet pipe 22. A gas mixing cavity 24 is provided at the axis of the mixing shell 23. Arc-shaped through grooves 25 are provided on both sides of the inner wall of the gas mixing cavity 24.

[0029] The inner walls of both sides of the arc-shaped through groove 25 are provided with slots in the vertical direction, and arc-shaped airflow guide plates 26 are inserted and installed on the inner walls of the slots.

[0030] The top of the hybrid housing 23 is screwed with a sealing cover plate 27, and an L-shaped exhaust pipe 28 is connected to the top axis of the sealing cover plate 27. The end of the L-shaped exhaust pipe 28 away from the sealing cover plate 27 is sleeved on the end of the gas supply pipe 29.

[0031] When multiple gas sources are supplied simultaneously and continuously, by mixing and inputting multiple gases into the mixing shell 23, and using the arc-shaped airflow guide plate 26 for uniform dispersion and guidance, the gas can be mixed more evenly. As a result, after being delivered to the incubator 1, the gas can be dispersed more evenly, and the simulated environment is more realistic.

[0032] In this embodiment, the carbon dioxide storage bottle 7, the spare carbon dioxide bottle 8, the first gas storage bottle 9, and the second gas storage bottle 10 are first inserted into the support collar 5. The first pressure reducing valve 11 is then connected to the carbon dioxide storage bottle 7 and the spare carbon dioxide bottle 8. The first gas storage bottle 9 and the second gas storage bottle 10 are then connected to the second pressure reducing valve 17. After connecting the remaining components, they are connected to an external power source, and a Siemens S7-200SMART power supply is installed. The series of PLC controllers are connected to the first pressure sensor 13, the first solenoid valve 14, the second pressure sensor 19, and the second solenoid valve 20. The pressure sensor is a 4-20mA sensor with an output of 0-1.6Mpa. After the valve of the carbon dioxide storage bottle 7 is opened, the first pressure reducing valve 11 reduces the pressure of the high-pressure gas. The reduced-pressure gas passes through the first pressure sensor 13. The value fed back by the first pressure sensor 13 is used to adjust the first pressure reducing valve 11 to ensure the safety of the gas pressure. Then, the valve of the first gas storage bottle 9 is opened, and the second pressure reducing valve 17 reduces the pressure of the high-pressure gas. The reduced-pressure gas passes through the second pressure sensor 19. The value fed back by the second pressure sensor 19 is used to adjust the second pressure reducing valve 17 to ensure the safety of the gas pressure. After the output gas enters the mixing shell 23, it passes through the arc-shaped air guide plate 26 to disperse the gas. After the two gases mix, they are discharged from the top L-shaped exhaust pipe 28 into the culture chamber 1. Because the gas is mixed very evenly, the cell culture environment is simulated more realistically. Example 2:

[0033] A gas source switching structure for a carbon dioxide incubator, such as Figure 1-4 As shown, this embodiment makes the following additions based on embodiment 1: the first pressure sensor 13 is installed 10-20cm downstream of the first pressure reducing valve 11, and the second pressure sensor 19 is installed 10-20cm downstream of the second pressure reducing valve 17. The coil circuits of the first solenoid valve 14 and the second solenoid valve 20 are both connected in parallel with an RC absorption circuit, and the RC absorption circuit includes a 100Ω resistor and a 0.1μF capacitor to prevent the back electromotive force from damaging the PLC controller when the power is off.

[0034] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A gas source switching structure for a carbon dioxide incubator, comprising an incubator body (1) equipped with a sealed door (2), characterized in that, A fixed support plate (3) is bolted to the bottom of one side of the incubator (1), and the top outer wall of the fixed support plate (3) has equally spaced positioning grooves (4). A support collar (5) is provided above the fixed support plate (3), and a carbon dioxide storage bottle (7), a carbon dioxide spare bottle (8), a first gas storage bottle (9), and a second gas storage bottle (10) are respectively inserted and installed on the inner wall of the support collar (5). The ports of the carbon dioxide storage bottle (7) and the carbon dioxide spare bottle (8) are both screwed to a first pressure reducing valve (11), and one end of the first pressure reducing valve (11) is screwed to a second pressure reducing valve (10). A connecting pipe (12) is provided, a first pressure sensor (13) is installed in the middle of the first connecting pipe (12), and a first solenoid valve (14) is screwed to the end of the first connecting pipe (12) away from the first pressure reducing valve (11). A first T-shaped three-way pipe (15) is connected between the two first solenoid valves (14), and a first air inlet pipe (16) is sleeved on the other end of the first T-shaped three-way pipe (15). A second pressure reducing valve (17) is screwed to the ports of the first gas storage bottle (9) and the second gas storage bottle (10), and a second connecting pipe (18) is screwed to one end of the second pressure reducing valve (17). A second pressure sensor (19) is installed in the middle of the second connecting pipe (18), and a second solenoid valve (20) is screwed to the end of the second connecting pipe (18) away from the second pressure reducing valve (17). A second T-shaped three-way pipe (21) is connected between the two second solenoid valves (20), and a second air inlet pipe (22) is sleeved at the other end of the second T-shaped three-way pipe (21). An air supply pipe (29) is connected to the inner wall of one side of the bottom of the incubator (1). A gas mixing component is connected between the first air inlet pipe (16) and the second air inlet pipe (22). The first pressure sensor (13), the first solenoid valve (14), the second pressure sensor (19), and the second solenoid valve (20) are all connected to a PLC controller through signal lines, and the PLC controller is connected to an external power supply through wires.

2. The gas source switching structure for a carbon dioxide incubator according to claim 1, characterized in that, The inner wall of each support collar (5) is bonded with a protective sponge ring (6), and the inner wall dimensions of the protective sponge ring (6) are adapted to the outer wall dimensions of the carbon dioxide storage bottle (7), the carbon dioxide spare bottle (8), the first gas storage bottle (9), and the second gas storage bottle (10).

3. The gas source switching structure for a carbon dioxide incubator according to claim 1, characterized in that, The mixing component includes a mixing housing (23) that is connected to the first air intake pipe (16) and the second air intake pipe (22), and a mixing cavity (24) is provided at the axis of the mixing housing (23), and arc-shaped through grooves (25) are provided on both sides of the inner wall of the mixing cavity (24).

4. The gas source switching structure for a carbon dioxide incubator according to claim 3, characterized in that, The inner walls of both sides of the arc-shaped through groove (25) are provided with slots in the vertical direction, and an arc-shaped airflow guide plate (26) is inserted and installed on the inner wall of the slot.

5. The gas source switching structure for a carbon dioxide incubator according to claim 4, characterized in that, The top of the hybrid housing (23) is screwed with a sealing cover plate (27), and an L-shaped exhaust pipe (28) is connected to the top axis of the sealing cover plate (27). The end of the L-shaped exhaust pipe (28) away from the sealing cover plate (27) is sleeved on the end of the gas transmission pipe (29).

6. The gas source switching structure for a carbon dioxide incubator according to claim 1, characterized in that, The first pressure sensor (13) is installed 10-20cm downstream of the first pressure reducing valve (11), and the second pressure sensor (19) is installed 10-20cm downstream of the second pressure reducing valve (17).

7. The gas source switching structure for a carbon dioxide incubator according to claim 1, characterized in that, The coil circuits of the first solenoid valve (14) and the second solenoid valve (20) both need to be connected in parallel with an RC absorption circuit, and the RC absorption circuit includes a 100Ω resistor and a 0.1μF capacitor to prevent the back electromotive force from damaging the PLC controller when the power is off.

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