A repair device for immunohistochemical reactions

Abstract

The utility model discloses a kind of repair equipment for immunohistochemical reaction, it is related to biological medical technology field, for solving the problem of low repair efficiency to tissue sample, it include: repair inner container, repair cover plate on the repair inner container, a window and liquid inlet hole, liquid outlet hole are set on the repair cover plate, slide glass clamping piece;Wherein, the window is provided with slide glass support frame on two parallel edges, several support grooves are set on the slide glass support frame;The slide glass clamping piece includes slide glass holder body and slide glass holder chuck, the slide glass holder body starts to have several slide glass grooves, and elastic pressing piece is set in the front end of the slide glass groove, the elastic pressing piece is used to clamp the slide glass of tissue sample, the slide glass holder chuck is set in one side of the slide glass holder body, the two sides of the slide glass holder body are provided with protruding component, and the protruding component is used to with the support groove inlaying support.

Description

Technical Field

[0001] This utility model relates to the field of biomedical technology, and in particular to a repair device for immunohistochemical reactions. Background Technology

[0002] Immunohistochemistry (IHC) is a practical biological experimental technique that utilizes the principle of specific binding between antigens and antibodies, employing enzymes, proteins, or chemical dyes to visualize the expression of macromolecular proteins or genes in situ on tissue sections or cell slices. It is widely used in the pathological diagnosis of various diseases, including tumors and inflammation. With the development of digital pathology technology, automated immunohistochemical reaction equipment has emerged to improve pathological diagnosis, enabling identification and analysis based on the reacted tissue. To ensure accurate reaction of tissue samples, tissue sample repair is necessary, i.e., repairing the tissue samples using a repair solution. Currently, existing repair equipment in immunohistochemical reactions relies on a robotic arm to sequentially place each slide containing a tissue sample into the repair device for soaking. Commonly used repair devices are divided into multiple zones, with slides placed in individual zones for soaking. However, due to the need to repair a large number of tissue samples, moving the robotic arm to each zone requires numerous operations, and simultaneous repair of a large number of tissue samples is not possible, reducing the efficiency of tissue sample repair. Summary of the Invention

[0003] In view of this, the present invention provides a repair device for immunohistochemical reactions, the main purpose of which is to solve the problem of low repair efficiency of existing tissue samples.

[0004] According to one aspect of this utility model, a repair device for immunohistochemical reactions is provided, comprising:

[0005] Repair inner liner, repair cover plate placed on the repair inner liner, the repair cover plate having a window and liquid inlet hole and liquid outlet hole, and glass slide holder;

[0006] Among them, a glass slide support frame is provided on the two parallel sides of the window, and the glass slide support frame is provided with several support grooves;

[0007] The slide holder includes a slide frame and a slide holder clamp. The slide frame has several slide slots for holding slides to be repaired. The slide holder clamp is located on one side of the slide frame. The slide frame has protruding parts on both sides for engaging with the support slots.

[0008] Preferably, the slide holder clamp and the slide are on the same horizontal and opposite surface.

[0009] Preferably, the support groove includes a first platform, a second platform, and a third platform. The first platform is connected to the second platform, the second platform is connected to the third platform, and the first platform is connected to the first bottom surface of the support groove. The support groove also includes a side surface, which is perpendicular to the first bottom surface. The first platform and the second platform are at a first angle.

[0010] Preferably, the protrusion includes a fourth platform, a fifth platform, and a sixth platform. The fourth platform is connected to the fifth platform, the fifth platform is connected to the sixth platform, and the fourth platform is connected to the top surface of the protrusion. The protrusion also includes a seventh platform and an eighth platform. The seventh platform is connected to the top surface of the protrusion, and the eighth platform is connected to the second bottom surface of the protrusion. The fourth platform and the fifth platform are at a second angle, and the first angle and the second angle are complementary.

[0011] Preferably, an elastic clamp is provided at the front end of the slide groove, the elastic clamp is fixedly connected to the slide holder, and the elastic clamp is used to hold the slide of the tissue sample.

[0012] Preferably, the slide holder clamp is either separately connected to or integrally connected to the slide holder body.

[0013] Preferably, the slide holder chuck has slots on both sides, which are used by the grippers on the robotic arm to clamp the slide holder chuck.

[0014] Preferably, the repair cover plate is connected to a cover body via a rotating component, the cover body being a cavity, and the coverage area of ​​the cover body being larger than the opening area of ​​the window.

[0015] Preferably, the inner wall of the inner bladder cavity has an inlet area below the inlet hole for the flow of repair fluid into the repair inner bladder.

[0016] By employing the above technical solutions, the technical solutions provided by the embodiments of this utility model have at least the following advantages:

[0017] This invention provides a repair device for immunohistochemical reactions. Compared with the prior art, the embodiment of this invention includes a repair inner liner, a repair cover plate placed on the repair inner liner, a window and an inlet / outlet on the repair cover plate, and a slide holder. A slide support frame is provided on two parallel sides of the window, and the slide support frame has several support grooves. The slide holder includes a slide frame body and a slide holder clamp. The slide frame body has several slide grooves, and the slide holder clamp is located on one side of the slide frame body. Protrusions are provided on both sides of the slide frame body for engaging with the support grooves. By providing a slide support frame with multiple support grooves on both sides of the repair inner liner, and engaging with the slide holder that can hold multiple slides, a large number of tissue samples can be placed in the repair solution simultaneously, thereby increasing the number of samples repaired within the same time period.

[0018] The above description is merely an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this utility model more obvious and understandable, specific embodiments of this utility model are given below. Attached Figure Description

[0019] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of the invention. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings:

[0020] Figure 1 An axial view of a repair device provided in an embodiment of the present invention is shown;

[0021] Figure 2 This shows a front view of a glass slide holder provided in an embodiment of the present invention;

[0022] Figure 3 A top view of a glass slide holder provided in an embodiment of the present invention is shown;

[0023] Figure 4 An axial view of a glass slide holder provided in an embodiment of the present invention is shown;

[0024] Figure 5 An axial view of a support groove provided in an embodiment of the present invention is shown;

[0025] Figure 6 This figure shows a side view of a glass slide clamping device provided by an embodiment of the present invention;

[0026] Figure 7This figure shows a front view of a robotic arm gripper provided in an embodiment of the present invention;

[0027] Figure 8 An axial view of a cover body provided in an embodiment of the present invention is shown;

[0028] Among them, the repair inner liner-1, repair cover plate-2, window-3, liquid inlet-4, liquid outlet-5, slide holder-6, slide support frame-7, support groove-71, slide frame body-61, slide frame chuck-62, slide groove-611, protruding component-612, first side-613, second side-614, third side-615, fourth side-616, fifth side-617, first tabletop-711, second tabletop-712, third tabletop- 713, side of the tank - 714, first bottom surface - 715, fourth platform - 6121, fifth platform - 6122, sixth platform - 6123, seventh platform - 6124, eighth platform - 6125, top surface - 6126, second bottom surface - 6127, elastic pressure plate - 617, slot - 621, chuck - 622, claw body - 623, cover body - 8, liquid inlet area - 9, robotic arm gripper - 10, first gripper side - 1001, second gripper side - 1002. Detailed Implementation

[0029] Exemplary embodiments of the present disclosure will now be described in more detail with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

[0030] In this embodiment of the invention, all directional indicators (including but not limited to up, down, left, right, front, back, etc.) are only used to explain the relative positional relationship and movement of the components in a specific posture (as shown in the attached figures). If the specific posture changes, the directional indicators will also change accordingly. Furthermore, in this embodiment of the invention, descriptions involving "first," "second," etc., are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first" or "second" may explicitly or implicitly include at least one feature. In the description of this invention, "several" means at least two, for example, two, three, etc., unless otherwise explicitly specified.

[0031] In this embodiment of the invention, unless otherwise explicitly specified and limited, the terms "connection," "fixing," etc., should be interpreted broadly. For example, "fixing" can mean a fixed connection, a detachable connection, or an integral part; "connection" can mean a mechanical connection, an electrical connection, a direct connection, or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the operational relationship between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this embodiment of the invention according to the specific circumstances.

[0032] This utility model embodiment provides a repair device for immunohistochemical reactions, such as... Figure 1 The repair device shown is a front view of the axis. The device includes: a repair liner 1, a repair cover plate 2 placed on the repair liner, a window 3, an inlet hole 4, an outlet hole 5, and a glass slide holder 6.

[0033] Among them, a glass slide support frame 7 is provided on the two parallel sides of the window 3, and a plurality of support grooves 71 are provided on the glass slide support frame 7.

[0034] The slide holder 6 includes a slide frame 61 and a slide holder clamp 62. The slide frame 61 has a plurality of slide grooves 611. The slide holder clamp 62 is disposed on one side of the slide frame. The slide frame 61 has protruding parts 612 on both sides. The protruding parts 612 are used to engage with the support groove 71 for support.

[0035] In this embodiment of the invention, the repair inner liner 1 is made of metal. The cavity of the repair inner liner 1 is used to hold the repair fluid for repairing tissue samples. A repair cover plate 2 is provided on the repair inner liner 1, and a window 3 is provided on the repair cover plate 2. The length and width of the window 3 are the same as the length and width of the cavity of the repair inner liner 1. The depth of the repair inner liner 1 can be set based on the length of the glass slide, and this embodiment of the invention does not make a specific limitation. In addition to the window 3, the repair cover plate 2 also has an inlet hole 4 and an outlet hole 5. At this time, the inlet hole 4 is used to inject the repair fluid into the cavity of the repair inner liner 1, and the outlet hole 5 is used to pump the repair fluid out of the cavity. The positions of the inlet hole 4 and the outlet hole 5 can be set on the same side or diagonally, and this invention does not make a specific limitation. In addition, slide support frames 7 are provided on two parallel sides of the window 3. The slide support frames 7 have several support slots 71. To increase the number of slides that can be loaded, the number of support slots 71 is preferably set such that a corresponding number of slide clamping members 6 can be fitted and supported. This embodiment of the invention does not impose a specific limitation. The slide clamping member 6 includes a slide frame body 61 and a slide frame clamp 62. As shown in Figure 2, the slide frame body 61 has several slide slots 611 for holding slides. The slide frame clamp 62 is located on one side of the slide frame body 61, i.e., the side not containing the slide slots 611. At this time, the slide frame clamp 62 can be moved by the corresponding grippers on the robotic arm, placing the slide clamping member 6 carrying multiple slides onto the slide support frame 7 of the window 3. In a specific implementation scenario, a slide holder 6 has five slide slots 611 on its slide frame 61 and 20 support slots 71 on one side of its slide support frame 7, enabling the restoration device to restore 200 tissue samples simultaneously. Furthermore, to ensure the slide holder 6 is stably positioned at the window 3, protruding components 612 are provided on both sides of the slide frame 61. These protruding components 612 engage with the support slots 71 for support, allowing the slides on the slide holder 6 to suspend in the restoration fluid.

[0036] In one embodiment of this utility model, when the slide holder 6 is freely and flexibly gripped by the bayonet of the robotic arm, the slide holder chuck 62 can be configured on the first side 613, second side 614, third side 615, fourth side 616, and fifth side 617 of the slide holder body 61, such as... Figure 3As shown, when the slide holder 62 is positioned on the third side 615 and the fifth side 617, it shares a side with the protruding parts 612-612. This embodiment of the invention does not impose specific limitations. Additionally, the fourth side 616 is the same side as the side where the slide groove 611 is located. To enable the robotic arm to move within a minimal space and adapt to a more optimized immunohistochemical reaction environment, preferably, the slide holder 62 and the slide are on the same horizontal and opposite surface, i.e., on the first side 613, as shown... Figure 4 The three-dimensional view shown is illustrated. Depending on the spatial environment in which the robotic arm operates, the slide holder 62 can be positioned near the edge of the slide holder 6, or it can be positioned in the middle of the slide holder 6. In this case, the slide holder 62 and the slide holder 6 can be integrally formed, or they can be manufactured separately and then fixedly connected, such as by screws. This embodiment of the invention does not impose specific limitations.

[0037] In one embodiment of this utility model, such as Figure 5 As shown, in order to stably place the slide holder 6 in the support groove 71, the support groove 71 includes a first platform 711, a second platform 712, and a third platform 713. The first platform 711 is connected to the second platform 712, the second platform 712 is connected to the third platform 713, and the first platform 711 is connected to the first bottom surface 715 of the support groove 71. The support groove 71 also includes a groove side surface 714, which is perpendicular to the first bottom surface 715. The first platform 711 and the second platform 712 are at a first angle.

[0038] In this embodiment, the first platform 711 can be directly connected to the first bottom surface 715 of the support groove 71. Correspondingly, the first platform 711 is connected to the second platform 712. To improve the stability of the fit, the first platform 711 and the second platform 712 are at a first angle, preferably 60 degrees, 70 degrees, etc., which are not specifically limited in this embodiment. Furthermore, the second platform 712 is connected to the third platform 713, which is parallel to the first bottom surface 715. It should be noted that the length and width of each platform can be configured based on the width of the support groove 71 within the slide support frame 7 and the thickness of the slide clamping member 6 (including the thickness of the slide groove 611 and the slide, or based on the thickness of the slide frame 61 of the slide clamping member 6). These dimensions are not specifically limited in this embodiment.

[0039] In one embodiment of this invention, to increase the stability of the fit and the safety of the glass slide holder 6, as shown in Figure 6, the protrusion includes a fourth platform 6121, a fifth platform 6122, and a sixth platform 6123. The fourth platform 6121 is connected to the fifth platform 6122, and the fourth platform 6121 and the fifth platform 6122 are at a second angle. The first angle and the second angle are complementary, so that the protrusion 612 can be stably fitted into the support groove 71. In addition, the fifth platform 6122 is connected to the sixth platform 6123, and the fourth platform 6121 is connected to the top surface 6126 of the protrusion, which can be vertically arranged to achieve stable fitting into the support groove 71. In addition, the protrusion also includes a seventh platform 6124 and an eighth platform 6125. The seventh platform 6124 is connected to the top surface 6126 of the protrusion, and the eighth platform 6125 is connected to the second bottom surface 6127 of the protrusion, so as to stably place the slide holder in other positions.

[0040] In one embodiment of this invention, in order to suspend the glass slide in the restoration fluid, an elastic pressure plate 617 is provided at the front end of the slide groove 611, such as... Figure 2 As shown, at this time, the elastic clamp 617 is fixedly connected to the slide holder 61, and the elastic clamp 617 is used to hold the slide of the tissue sample. Preferably, the elastic clamp 617 is fixed to the slide holder 61 by screws. Since it is necessary to suspend the slide in the restoration solution and reduce the space occupied on the slide holder 61, the slide groove 611 is designed as follows... Figure 2 As shown, the elastic flattening device can be fixed to the front end of the slide groove 611 to press the glass slide placed in the slide groove 611. Furthermore, the elastic pressing plate 617 can be made of elastic metal, plastic, etc., and this embodiment of the invention does not impose specific limitations.

[0041] In one embodiment of the present invention, in order to clean or flexibly install the repair inner liner 1, preferably, the repair inner liner 1 and the repair cover plate 2 are connected separately or as a whole. The separate connection can be a detachable connection method, and the integrated connection can be an integrated manufacturing method. The present invention does not make specific limitations.

[0042] In one embodiment of this utility model, to stabilize the gripping action of the robotic arm, preferably, slots 621 are provided on both sides of the slide holder chuck 62. These slots 621 are used by the grippers on the robotic arm to clamp and hold the slide holder chuck 62. Figure 2As shown, the slots 621 on both sides are symmetrically arranged. They can be slots 621 formed by two platforms at a specific angle, or they can be right-angled slots 621 formed by three platforms. This embodiment of the invention does not impose a specific limitation. Furthermore, to accommodate the robotic arm's gripping capabilities, as shown in Figure 7, the gripper 10 on the robotic arm includes a first gripper side 1001 and a second gripper side 1002. The first gripper side 1001 and the second gripper side 1002 are provided with protruding surfaces complementary to the shape of the slots 621, forming claw bodies 623 to facilitate engagement and gripping of the slots 621. For example, if the slot 621 is a slot 621 formed by two platforms at a specific angle, then the claw body 623 formed by the corresponding protruding surface of the gripper is formed at an angle complementary to the specific angle. This embodiment of the invention does not impose a specific limitation.

[0043] In one embodiment of this invention, in order to protect the tissue repair environment for immunohistochemical staining and ensure repair effectiveness, such as... Figure 8 As shown, a cover 8 is connected to the repair cover plate 2 via a rotating component. The cover 8 is a cavity, and its height can be configured based on the equipment environment, preferably 1 cm. In this case, the coverage area of ​​the cover 8 is larger than the opening area of ​​the window 3, that is, the length of the cover 8 is greater than the length of the window 3, and the width of the cover 8 is greater than the width of the window 3, so that the cover 8 can completely cover the window 3. In addition, the cover 8 can be connected by installing a rotating component on one side to achieve the purpose of opening and closing. This embodiment of the utility model does not make specific limitations.

[0044] In one embodiment of this invention, since the glass slide is suspended in the repair fluid after being held by the glass slide holder 6, in order to save repair fluid, the length and width of the inner cavity of the repair liner 1 that carries the repair fluid are the same as the length and width of the window 3, and the height can be set based on the equipment environment. The liquid outlet pipe can enter the inner cavity of the inner cavity through the liquid outlet hole 5. This embodiment of the invention does not make specific limitations. Among them, the inner wall of the inner cavity is provided with a liquid inlet area 9 below the liquid inlet hole 4, as shown in Figure 2. The liquid inlet area 9 is used for the flow of repair fluid into the repair liner 1. The length, width, and height of the liquid inlet area 9 can be flexibly set according to the inner cavity of the inner cavity and the thickness of the inner wall of the repair liner 1. This embodiment of the invention does not make specific limitations.

[0045] In addition, it should be noted that the materials used in the embodiments of this utility model are all metal unless otherwise specified, and no specific limitations are imposed.

[0046] This invention provides a repair device for immunohistochemical reactions. By setting up a slide support frame with multiple support grooves on both sides of the repair inner liner and fitting it with a slide clamping component that can hold multiple slides, a large number of tissue samples can be placed in the repair solution, thereby increasing the number of samples repaired.

[0047] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A repair device for immunohistochemical reactions, characterized in that, include: Repair inner liner, repair cover plate placed on the repair inner liner, the repair cover plate having a window and liquid inlet hole and liquid outlet hole, and glass slide holder; Among them, a glass slide support frame is provided on the two parallel sides of the window, and the glass slide support frame is provided with several support grooves; The slide holder includes a slide frame and a slide holder clamp. The slide frame has several slide slots for holding slides to be repaired. The slide holder clamp is located on one side of the slide frame. The slide frame has protruding parts on both sides for engaging with the support slots.

2. The repair equipment according to claim 1, characterized in that, The slide holder clamp and the glass slide are on the same horizontal and opposite plane.

3. The repair equipment according to claim 1, characterized in that, The support groove includes a first platform, a second platform, and a third platform. The first platform is connected to the second platform, the second platform is connected to the third platform, and the first platform is connected to the first bottom surface of the support groove. The support groove also includes a side surface, which is perpendicular to the first bottom surface. The first platform and the second platform are at a first angle.

4. The repair equipment according to claim 3, characterized in that, The protrusion includes a fourth platform, a fifth platform, and a sixth platform. The fourth platform is connected to the fifth platform, the fifth platform is connected to the sixth platform, and the fourth platform is connected to the top surface of the protrusion. The protrusion also includes a seventh platform and an eighth platform. The seventh platform is connected to the top surface of the protrusion, and the eighth platform is connected to the second bottom surface of the protrusion. The fourth platform and the fifth platform are at a second angle, and the first angle and the second angle are complementary.

5. The repair equipment according to claim 1, characterized in that, An elastic clamp is provided at the front end of the slide slot. The elastic clamp is fixedly connected to the slide holder and is used to hold the slide of the tissue sample.

6. The repair equipment according to claim 1, characterized in that, The slide holder clamp is either separately connected to the slide holder body or integrally connected.

7. The repair equipment according to claim 1, characterized in that, The repaired inner liner and the repaired cover plate are either connected separately or as a single unit.

8. The repair equipment according to claim 1, characterized in that, The slide holder chuck has slots on both sides, which are used by the grippers on the robotic arm to clamp the slide holder chuck.

9. The repair equipment according to claim 1, characterized in that, The repair cover plate is connected to a cover body via a rotating component. The cover body is a cavity, and the coverage area of ​​the cover body is larger than the opening area of ​​the window.

10. The repair equipment according to claim 1, characterized in that, The inner liner of the repaired liner has an inlet area in the inner wall of the liner below the inlet hole, for the flow of repair fluid into the repaired liner.

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