A cerebrospinal fluid cryogenic storage tube

Abstract

The utility model discloses a kind of cerebrospinal fluid cryopreservation tubes, including outer tube and inner tube, two cavities along outer tube center line are equipped in outer tube, one is centrifugal tube cavity, another is cryopreservation tube cavity, inner tube is used to hold cerebrospinal fluid, can be placed in any cavity, inner tube cover and outer tube cover are detachably connected, micropore that can filter supernatant is opened in inner tube bottom portion.The utility model has the dual function of centrifugal tube and cryopreservation tube, and the cell precipitate (cerebrospinal fluid has type component) after centrifugation is preserved together with supernatant, and it is convenient to use.

Description

Technical Field

[0001] This utility model relates to the field of cell culture device technology, specifically to a cerebrospinal fluid cryopreservation tube. Background Technology

[0002] Cerebrospinal fluid (CSF) cryopreservation is a technique for preserving CSF samples at low temperatures, primarily for subsequent cell analysis, biomarker research, or disease diagnosis. The cryopreservation steps are as follows: 1. Collect CSF; 2. Centrifuge and remove the supernatant, retaining the cell pellet (the formed components of CSF); 3. Resuspend the cells in an appropriate amount of cryopreservation solution and aliquot them into cryovials. The cryopreservation process requires centrifuge tubes, cryovials, and supernatant storage tubes. After centrifuging the collected CSF in the centrifuge tubes, a special device is needed to extract the supernatant from the centrifuge tubes. Then, the cell pellet is transferred to cryovials for freezing. This process is cumbersome, and the cryopreservation solution and supernatant are stored separately, requiring separate retrieval for subsequent analysis. Each sample requires a unique pair of cryopreservation solutions and supernatants, which is quite inconvenient. Utility Model Content

[0003] This invention provides a cerebrospinal fluid cryopreservation tube that has the dual functions of a centrifuge tube and a cryopreservation tube, and preserves the centrifuged cell precipitate (the solid component of cerebrospinal fluid) together with the supernatant, making it convenient to use.

[0004] To achieve the above-mentioned technical objectives, the technical solution adopted by this utility model is as follows:

[0005] A cerebrospinal fluid cryopreservation tube includes an outer tube and an inner tube. The outer tube has two symmetrical lumens along its center line, one for centrifugation and the other for cryopreservation. The inner tube is used to hold cerebrospinal fluid and can be placed in either lumen. The cap of the inner tube is detachably connected to the cap of the outer tube. The bottom of the inner tube has micropores for filtering the supernatant.

[0006] Furthermore, the two lumens are a long lumen and a short lumen, respectively. The long lumen is used for centrifugation, and the short lumen is used for cryopreservation. The short lumen can hold cell cryopreservation solution.

[0007] Furthermore, the long and short lumens have the same diameter.

[0008] Furthermore, the diameter of the micropore is 0.4 μm.

[0009] Furthermore, both the outer and inner tubes are made of plastic.

[0010] Furthermore, the bottom of the outer tube cap is provided with a groove, the inner wall of the groove is provided with an internal thread, the height of the inner tube cap is greater than the depth of the groove, the outer wall of the inner tube cap is provided with an external thread, and the outer tube cap and the inner tube cap are threadedly connected.

[0011] Furthermore, the inner tube cap has a diameter larger than the tube cavity, and a sealing ring is provided at the bottom of the inner tube cap.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0013] This invention has the dual functions of a centrifuge tube and a cryopreservation tube. The inner tube contains cerebrospinal fluid and is placed in the long tube. After the outer tube is centrifuged, the supernatant is filtered through a micropore and retained in the long tube. Cell cryopreservation solution is added to the short tube. The inner tube containing cell precipitate (the formed component of cerebrospinal fluid) is then transferred to the short tube to achieve cryopreservation. This invention can preserve the centrifuged cell precipitate (the formed component of cerebrospinal fluid) and supernatant together in the outer tube, making it convenient to use. Attached Figure Description

[0014] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the structure of this utility model before centrifugation;

[0016] Figure 2 This is a schematic diagram of the structure of the present invention after centrifugation;

[0017] In the diagram: 1-outer tube, 2-inner tube, 3-cerebrospinal fluid, 4-micropore, 5-long lumen, 6-short lumen, 7-cell cryopreservation solution, 8-groove, 9-supernatant, 10-cell pellet. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0019] In the description of the embodiments of this application, it should be noted that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship that the product of this application is usually placed in when in use, or the orientation or positional relationship that is commonly understood by those skilled in the art. It is only for the convenience of describing this application and simplifying the description, and is not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this application.

[0020] In the description of the embodiments of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set," "install," and "connect" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0021] As shown in the figure, a cerebrospinal fluid cryopreservation tube includes an outer tube 1 and an inner tube 2. The outer tube 1 has two symmetrical lumens along its center line. The inner tube 2 is used to hold cerebrospinal fluid 3. One lumen is a long lumen 5, used as a centrifugation lumen, and the other is a short lumen 6, which can hold cell cryopreservation solution 7 and is used as a cryopreservation lumen. The long lumen 5 and the short lumen 6 have the same diameter. The inner tube 2 can be placed in either lumen. The bottom of the inner tube 2 has micropores 4 for filtering the supernatant. Preferably, the diameter of the micropores 4 is 0.4 μm. Preferably, both the outer tube 1 and the inner tube 2 are made of plastic.

[0022] The inner tube 2 cap and the outer tube 1 cap are detachably connected. Preferably, the bottom of the outer tube 1 cap has a groove 8, the inner wall of the groove 8 has an internal thread, the height of the inner tube 2 cap is greater than the depth of the groove 8, the outer wall of the inner tube 2 cap has an external thread, and the outer tube 1 cap and the inner tube 2 cap are threaded together. The diameter of the inner tube 2 cap is larger than the tube cavity, and the bottom of the inner tube 2 cap has a sealing ring.

[0023] This invention features dual functions as a centrifuge tube and a cryopreservation tube. In use, the collected cerebrospinal fluid 3 is placed in the inner tube 2, and the inner tube 2 is connected to the cap of the outer tube 1. The inner tube 2 is then placed in the long tube lumen 5, and the cap of the outer tube 1 is connected to the tube body. Preferably, the cap and body of the outer tube 1 are connected by a snap-fit. After centrifugation, the supernatant 9 (small molecules) is filtered through micropores and retained in the long tube lumen 5, while the cell precipitate 10 remains in the inner tube 2. The cap of the outer tube 1 is opened, and cell cryopreservation solution 7 is added to the short tube lumen 6. The cap of the outer tube 1, along with the inner tube 2, is rotated 180°, and the inner tube 2 is inserted into the short tube lumen 6. The cell cryopreservation solution 7 passes through the micropores 4 and encapsulates the cell precipitate 10, thus achieving cell cryopreservation. This invention can preserve the centrifuged cell precipitate 10 (the formed components of cerebrospinal fluid) and the supernatant 9 together in the outer tube 1, making it convenient to use.

[0024] Of course, there may be other embodiments of this utility model. Without departing from the spirit and essence of this utility model, those skilled in the art can make various corresponding changes and modifications based on this utility model, but these corresponding changes and modifications should all fall within the protection scope of the appended claims of this utility model.

Claims

1. A cerebrospinal fluid cryopreservation tube, characterized in that: It includes an outer tube (1) and an inner tube (2). The outer tube (1) has two symmetrical cavities along the center line of the outer tube (1), one for centrifugation and the other for cryopreservation. The inner tube (2) is used to hold cerebrospinal fluid (3) and can be placed in either cavity. The cap of the inner tube (2) is detachably connected to the cap of the outer tube (1). The bottom of the inner tube (2) has micropores (4) for filtering the supernatant.

2. The cerebrospinal fluid cryopreservation tube according to claim 1, characterized in that: The two lumens are a long lumen (5) and a short lumen (6), respectively. The long lumen (5) is for centrifugation, and the short lumen (6) is for cryopreservation. The short lumen (6) can hold cell cryopreservation solution (7).

3. The cerebrospinal fluid cryopreservation tube according to claim 2, characterized in that: The long tube (5) and the short tube (6) have the same diameter.

4. The cerebrospinal fluid cryopreservation tube according to claim 1, characterized in that: The diameter of the micropore (4) is 0.4 μm.

5. The cerebrospinal fluid cryopreservation tube according to claim 1, characterized in that: Both the outer tube (1) and the inner tube (2) are plastic tubes.

6. The cerebrospinal fluid cryopreservation tube according to claim 1, characterized in that: The bottom of the outer tube (1) cap is provided with a groove (8), the inner wall of the groove (8) is provided with an internal thread, the height of the inner tube (2) cap is greater than the depth of the groove (8), the outer wall of the inner tube (2) cap is provided with an external thread, and the outer tube (1) cap and the inner tube (2) cap are threadedly connected.

7. The cerebrospinal fluid cryopreservation tube according to claim 1, characterized in that: The inner tube (2) has a cap diameter larger than the tube cavity, and a sealing ring is provided at the bottom of the inner tube (2) cap.

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