Biological cultivation device of bionic bezoar

Abstract

The utility model discloses a kind of biological cultivation devices of bionic bovine bezoar, comprising: strain separation and purification module, including constant-temperature culture unit and strain low-temperature storage unit;Primary fermentation module, including primary fermentation unit and primary centrifugal unit, the input end of primary fermentation unit is connected with strain low-temperature storage unit, the input end of primary centrifugal unit is connected with primary fermentation unit;Bile pretreatment module, including sterilization unit and bile low-temperature storage unit;Secondary fermentation and composite bilirubin calcium preparation module, including sequentially connected secondary fermentation unit, heating unit and secondary centrifugal unit, the input end of secondary fermentation unit is connected with primary centrifugal unit and bile low-temperature storage unit respectively;Bionic cultivation module, including sequentially connected mixing unit, promote unit and first freeze-drying unit, the input end of mixing unit is connected with secondary centrifugal unit;Bionic shaping module, including shaping unit, the input end of shaping unit is connected with first freeze-drying unit;Spraying module, including sequentially connected spraying unit and second freeze-drying unit, the input end of spraying unit is connected with shaping unit.The utility model simplifies the cultivation process of bionic bovine bezoar, reduces the production cost of bionic bovine bezoar.

Description

Technical Field

[0001] This utility model relates to the field of biomedical technology, and more specifically, to a biomimetic bezoar biological cultivation device. Background Technology

[0002] Bezoar is a precious traditional Chinese medicine, widely used in over 600 kinds of traditional Chinese medicine preparations, including Pien Tze Huang. Due to the extremely low probability of natural bezoar formation (0.21%-0.68%) and limited production, the market supply and demand imbalance is significant. Current technologies for in vitro culture of bezoar involve complex processes, high costs, and insufficient output to meet market demand. Therefore, there is an urgent need for a biomimetic bezoar culture device that is simple in structure, easy to operate, and suitable for large-scale production. Utility Model Content

[0003] The main purpose of this invention is to provide a biomimetic bezoar biological cultivation device to solve the problems of complex production process, high cost, and difficulty in meeting market demand in the production of in vitro cultured bezoar in related technologies.

[0004] To achieve the above objectives, this utility model provides a biomimetic bezoar biological cultivation device, comprising:

[0005] The strain isolation and purification module includes a constant temperature culture unit and a strain low temperature preservation unit, wherein the input end of the strain low temperature preservation unit is connected to the constant temperature culture unit.

[0006] A primary fermentation module includes a primary fermentation unit and a primary centrifugation unit. The input end of the primary fermentation unit is connected to the strain low-temperature preservation unit, and the input end of the primary centrifugation unit is connected to the primary fermentation unit.

[0007] The bile pretreatment module includes a sterilization unit and a bile cryopreservation unit, wherein the input end of the bile cryopreservation unit is connected to the sterilization unit.

[0008] The secondary fermentation and compound bilirubin calcium preparation module includes a secondary fermentation unit, a heating unit and a secondary centrifugation unit connected in sequence. The input end of the secondary fermentation unit is connected to the primary centrifugation unit and the bile low-temperature preservation unit, respectively.

[0009] The biomimetic cultivation module includes a mixing unit, a catalytic unit, and a first freeze-drying unit connected in sequence, with the input end of the mixing unit connected to the secondary centrifugation unit;

[0010] A biomimetic molding module includes a molding unit, the input end of which is connected to the powder outlet of the first freeze-drying unit;

[0011] The spraying module includes a spraying unit and a second freeze-drying unit connected in sequence. The input end of the spraying unit is connected to the forming unit via a conveyor belt.

[0012] Furthermore, the input end of the secondary fermentation unit is connected to the primary centrifugation unit and the bile cryopreservation unit respectively via sterile pipes;

[0013] The input end of the heating unit is connected to the secondary fermentation unit via a sterile pipe;

[0014] The input end of the mixing unit is connected to the secondary centrifugation unit via a sterile pipe;

[0015] The output end of the triggering unit is connected to the first freeze-drying unit via a sterile pipe;

[0016] The input end of the molding unit is connected to the powder outlet of the second freeze-drying unit via a sterile pipe;

[0017] The spraying unit and the second freeze-drying unit are connected by a conveyor belt.

[0018] Furthermore, both the primary fermentation unit and the secondary fermentation unit include a biological thermostatic shaker or a stirred fermenter.

[0019] Furthermore, the tank body of the biological constant temperature shaker or stirred fermenter is made of non-metallic materials.

[0020] Furthermore, the sterilization unit includes a high-temperature, high-pressure, moist heat sterilizer, and the input end of the bile cryopreservation unit is connected to the high-temperature, high-pressure, moist heat sterilizer via a sterile pipe.

[0021] Furthermore, the bile cryopreservation unit includes a bile cryopreservation tank.

[0022] Furthermore, the body of the bile cryogenic storage tank is made of non-metallic materials.

[0023] Furthermore, both the first freeze-drying unit and the second freeze-drying unit include a freeze dryer.

[0024] Furthermore, both the primary and secondary centrifugation units include centrifuges.

[0025] Furthermore, the initiation unit includes an acid-base monitor and an initiator dripping device.

[0026] Furthermore, the forming unit includes a pelletizing machine.

[0027] Furthermore, the spraying unit is suspended directly above the conveyor belt and is used to spray the wet shot on the conveyor belt below.

[0028] The biomimetic bezoar cultivation device provided in this embodiment of the invention simplifies the cultivation process and reduces production costs by utilizing a modular design and multiple modules and equipment units working together. The multiple modules, arranged in a corresponding sequence, can form an assembly line production line, suitable for large-scale industrial production, effectively addressing market supply and demand imbalances, and yielding significant economic and social benefits. Furthermore, the primary fermentation module pre-ferments the bacterial strains during production, greatly increasing the number of strains and thus improving fermentation efficiency in the secondary fermentation module, thereby enhancing the overall production efficiency. Simultaneously, the formed bezoar product can be surface-coated using a spraying unit, providing better protection and facilitating subsequent storage and use. Attached Figure Description

[0029] The accompanying drawings, which form part of this utility model, are used to provide a further understanding of the utility model, making other features, objects, and advantages of the utility model more apparent. The illustrative embodiments of the utility model and their descriptions are used to explain the utility model and do not constitute an undue limitation of the utility model. In the drawings:

[0030] Figure 1 This is a schematic diagram of the arrangement of the biomimetic bezoar biological cultivation device according to an embodiment of this utility model;

[0031] The module includes: 1. Strawberry isolation and purification module; 2. Primary fermentation module; 3. Bile pretreatment module; 4. Secondary fermentation and compound bilirubin calcium preparation module; 5. Bionic cultivation module; 6. Bionic molding module; 7. Spraying module. Detailed Implementation

[0032] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the protection scope of the present invention.

[0033] It should be noted that the terms "first," "second," etc., used in the specification, claims, and accompanying drawings of this utility model are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate for the embodiments of this utility model described herein.

[0034] In this invention, the terms "upper," "lower," "inner," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing this invention and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.

[0035] Furthermore, in addition to indicating direction or positional relationship, some of the aforementioned terms may also have other meanings. For example, the term "above" may also be used in some cases to indicate a certain dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this utility model according to the specific circumstances.

[0036] Furthermore, the terms "set up," "equipped with," "connected," and "fixed" should be interpreted broadly. For example, "connected" can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be an internal connection between two devices, components, or parts. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0037] In addition, the term "multiple" should mean two or more.

[0038] It should be noted that, where there is no conflict, the embodiments and features in the embodiments of this utility model can be combined with each other. The present utility model will now be described in detail with reference to the accompanying drawings and embodiments.

[0039] To solve related technical problems, such as Figure 1 As shown, this embodiment of the invention provides a biomimetic bezoar biological cultivation device, comprising:

[0040] The strain isolation and purification module 1 includes a constant temperature culture unit and a strain low temperature preservation unit, wherein the input end of the strain low temperature preservation unit is connected to the constant temperature culture unit.

[0041] The primary fermentation module 2 includes a primary fermentation unit and a primary centrifugation unit. The input end of the primary fermentation unit is connected to the strain low-temperature preservation unit, and the input end of the primary centrifugation unit is connected to the primary fermentation unit.

[0042] The bile pretreatment module 3 includes a sterilization unit and a bile cryopreservation unit, wherein the input end of the bile cryopreservation unit is connected to the sterilization unit.

[0043] The secondary fermentation and compound bilirubin calcium preparation module 4 includes a secondary fermentation unit, a heating unit, and a secondary centrifugation unit. The input end of the secondary fermentation unit is connected to the primary centrifugation unit and the bile cryopreservation unit, respectively.

[0044] The biomimetic cultivation module 5 includes a mixing unit, a catalytic unit, and a first freeze-drying unit connected in sequence, with the input end of the mixing unit connected to the secondary centrifugation unit;

[0045] The biomimetic molding module 6 includes a molding unit, the input end of which is connected to the powder outlet of the first freeze-drying unit;

[0046] The spraying module 7 includes a spraying unit and a second freeze-drying unit connected in sequence. The input end of the spraying unit is connected to the forming unit via a conveyor belt.

[0047] In this embodiment, the strain isolation and purification module 1 is used to isolate and purify luteogenic strains from fresh bovine bile or natural bezoar. It includes a constant-temperature culture unit and a strain cryopreservation unit. The constant-temperature culture unit is used to culture, isolate, and purify the luteogenic strains at a specific temperature, while the cryopreservation unit is used to preserve the purified luteogenic strains at 1-4°C. Specifically, the constant-temperature culture unit may include a centrifuge, culture medium, and a constant-temperature incubator. The luteogenic strains are isolated and purified from bovine bile using the centrifuge, and then cultured in the culture medium, with the constant-temperature incubator serving as the culture environment. The strain cryopreservation unit is used to cryopreserve the cultured luteogenic strains, specifically using an incubator capable of maintaining a low temperature.

[0048] The cultured flavonoid strain is then fermented in primary fermentation module 2, and the fermentation products are processed. Primary fermentation module 2 includes a primary fermentation unit and a primary centrifugation unit connected in sequence. In one embodiment, the primary fermentation unit is a bio-thermal shaker or a stirred fermenter, and the primary centrifugation unit is a centrifuge. The product fermented in the bio-thermal shaker or stirred fermenter enters the centrifuge for high-speed centrifugation separation.

[0049] The bile pretreatment module 3 includes a sterilization unit and a bile cryopreservation unit connected in sequence. Fresh bovine bile is first introduced into the sterilization unit for sterilization, and the sterilized bovine bile is then introduced into the bile cryopreservation unit for storage, facilitating subsequent use. In one embodiment, the sterilization unit may include a high-temperature, high-pressure, moist heat sterilizer, and the bile cryopreservation unit may employ appropriate equipment with cryopreservation capabilities, such as a cryogenic storage tank. The input end of the bile cryopreservation unit is connected to the high-temperature, high-pressure, moist heat sterilizer via a sterile pipeline. Furthermore, the bile can be purified before sterilization by using a filtration unit. Specifically, a multi-stage filtration unit can be used, with the pore size of the multi-stage filtration unit gradually decreasing.

[0050] The fermentation products and sterilized bile obtained from the primary fermentation module 2 need to enter the secondary fermentation and complex bilirubin calcium preparation module 4 for further fermentation. The secondary fermentation and complex bilirubin calcium preparation module 4 is a large-scale fermentation process, capable of handling a larger fermentation volume. Specifically, in this embodiment, the secondary fermentation and complex bilirubin calcium preparation module 4 includes a secondary fermentation unit, a heating unit, and a secondary centrifugation unit connected in sequence. The input end of the secondary fermentation unit is connected to the primary centrifugation unit and the bile cryopreservation unit, respectively. Specifically, bovine bile fermented by flavonoid strains can be sterilized, then a clear saturated calcium hydroxide solution and sodium metabisulfite can be added, heated to boiling, and then centrifuged to obtain complex bilirubin calcium. In one embodiment, the heating unit can be any device with heating function, such as a heating tank. In one embodiment, the secondary fermentation unit includes a biological constant-temperature shaker or a stirred fermentation tank, and the secondary centrifugation unit can be a centrifuge, capable of centrifuging the heated product.

[0051] The fermentation product obtained from the secondary fermentation module and the compound bilirubin calcium preparation module 4 is introduced into the biomimetic cultivation module 5 for biomimetic bezoar cultivation. This module includes a mixing unit, a priming unit, and a first freeze-drying unit connected in sequence. The input end of the mixing unit is connected to the secondary centrifugation unit. The biomimetic cultivation module 5 is used to process the fermentation product and cultivate biomimetic bezoar during the process. Specifically, the mixing unit mixes the compound bilirubin calcium obtained after boiling and centrifugation with the effective bezoar components produced during fermentation. In one embodiment, the mixing unit can be a mixing container, and the priming unit mainly uses a priming agent to promote the formation of biomimetic cultured bezoar after the pH is adjusted appropriately. The first freeze-drying unit is used to freeze-dry the formed biomimetic bezoar product, and a freeze dryer can be used. In one embodiment, the priming unit includes a pH monitor and a priming reagent adding device. The priming reagent adding device is used to add priming reagent to the priming agent, and the pH monitor can monitor the environmental pH value of the biomimetic cultured bezoar during the priming process.

[0052] The biomimetic bezoar prepared by the biomimetic cultivation module 5 is fed into the molding unit for molding. Specifically, in the molding unit, powdered biomimetic bezoar can be combined with masterbatch and formed into wet pellets by a pelletizing machine. In one embodiment, the molding unit includes a pelletizing machine.

[0053] The formed wet pellets undergo further processing via a spraying module 7. This process involves first spraying a black coating onto the surface of the wet pellets using a spraying unit within the spraying module 7, and then feeding them into a second freeze-drying unit for freeze-drying to obtain the finished product. In one embodiment, the second freeze-drying unit can also be a freeze dryer.

[0054] To improve production efficiency, the corresponding modules and units need to be connected by a conveyor structure to facilitate the rapid transfer of products from the upper level to the lower level. Therefore, in this embodiment, the input end of the secondary fermentation unit is connected to the primary centrifugation unit and the bile cryopreservation unit via sterile pipes; the input end of the heating unit is connected to the secondary fermentation unit via sterile pipes; the input end of the mixing unit is connected to the secondary centrifugation unit via sterile pipes; the output end of the activating unit is connected to the first freeze-drying unit via sterile pipes; the input end of the molding unit is connected to the powder outlet of the first freeze-drying unit via sterile pipes; and the input and output ends of the spraying unit are connected to the molding unit and the second freeze-drying unit via conveyor belts.

[0055] To improve production quality, the bodies of the bio-thermal shaker or stirred fermentation tank and the low-temperature bile storage tank in this embodiment are all made of non-metallic materials. Compared to tanks that are conventionally made of stainless steel, the tanks in this embodiment are made of non-metallic materials, thereby reducing the impact on the quality of the biomimetic bezoar product.

[0056] To facilitate spraying, the spraying unit is suspended directly above the conveyor belt for spraying wet shot on the conveyor belt below.

[0057] The above description is merely a preferred embodiment of this utility model and is not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A biomimetic bezoar-inspired biological cultivation device, characterized in that, include: The strain isolation and purification module includes a constant temperature culture unit and a strain low temperature preservation unit, wherein the input end of the strain low temperature preservation unit is connected to the constant temperature culture unit. A primary fermentation module includes a primary fermentation unit and a primary centrifugation unit. The input end of the primary fermentation unit is connected to the strain low-temperature preservation unit, and the input end of the primary centrifugation unit is connected to the primary fermentation unit. The bile pretreatment module includes a sterilization unit and a bile cryopreservation unit, wherein the input end of the bile cryopreservation unit is connected to the sterilization unit. The secondary fermentation and compound bilirubin calcium preparation module includes a secondary fermentation unit, a heating unit and a secondary centrifugation unit connected in sequence. The input end of the secondary fermentation unit is connected to the primary centrifugation unit and the bile low-temperature preservation unit, respectively. The biomimetic cultivation module includes a mixing unit, a catalytic unit, and a first freeze-drying unit connected in sequence, with the input end of the mixing unit connected to the secondary centrifugation unit; A biomimetic molding module includes a molding unit, the input end of which is connected to the powder outlet of the first freeze-drying unit; The spraying module includes a spraying unit and a second freeze-drying unit connected in sequence. The input end of the spraying unit is connected to the forming unit via a conveyor belt.

2. The biomimetic bezoar biological cultivation device according to claim 1, characterized in that, The input end of the secondary fermentation unit is connected to the primary centrifugation unit and the bile cryopreservation unit respectively via sterile pipes; The input end of the heating unit is connected to the secondary fermentation unit via a sterile pipe; The input end of the mixing unit is connected to the secondary centrifugation unit via a sterile pipe; The output end of the triggering unit is connected to the first freeze-drying unit via a sterile pipe; The input end of the molding unit is connected to the powder outlet of the first freeze-drying unit via a sterile pipe. The input and output ends of the spraying unit are connected to the molding unit and the second freeze-drying unit via conveyor belts, respectively.

3. The biomimetic bezoar biological cultivation device according to claim 1, characterized in that, Both the primary fermentation unit and the secondary fermentation unit include a biological thermostatic shaker or a stirred fermentation tank.

4. The biomimetic bezoar biological cultivation device according to claim 3, characterized in that, The body of the biological constant temperature shaker or stirred fermenter is made of non-metallic materials.

5. The biomimetic bezoar biological cultivation device according to claim 1, characterized in that, The sterilization unit includes a high-temperature, high-pressure, moist heat sterilizer, and the input end of the bile low-temperature preservation unit is connected to the high-temperature, high-pressure, moist heat sterilizer via a sterile pipe.

6. The biomimetic bezoar biological cultivation device according to claim 5, characterized in that, The bile cryopreservation unit includes a bile cryopreservation tank.

7. The biomimetic bezoar biological cultivation device according to claim 6, characterized in that, The body of the bile cryogenic storage tank is made of non-metallic materials.

8. The biomimetic bezoar biological cultivation device according to claim 1, characterized in that, Both the first freeze-drying unit and the second freeze-drying unit include a freeze dryer.

9. The biomimetic bezoar biological cultivation device according to claim 1, characterized in that, The priming unit includes an acid-base monitor and a priming reagent dispensing device; Both the primary centrifugation unit and the secondary centrifugation unit include centrifuges; The forming unit includes a pelletizing machine.

10. The biomimetic bezoar biological cultivation device according to claim 1, characterized in that, The spraying unit is suspended directly above the conveyor belt and is used to spray wet shot on the conveyor belt below.

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