Animal serum sample collection device with pre-cooling pipetting function

Abstract

The utility model provides a kind of animal serum sample collection device with precooling pipetting function, it is related to blood detection technical field, mainly for solving the problem of large blood sample separation loss in prior art. The animal serum sample collection device includes blood collection tube, pipette, cooling water jacket and pipette plunger, wherein, cooling water jacket is sleeved on blood collection tube, for cooling the blood in blood collection tube;Transparent pipette plunger can be sealingly inserted into blood collection tube, through the step hole is arranged in pipette plunger, small hole is slender in lower part, large hole in upper part can be inserted into pipette.The utility model separates serum, only about 1-3% of total serum is left in slender small hole, which greatly reduces the separation loss of serum sample.In addition, the utility model can also accurately remove gel layer to obtain pure blood cells, and the blood can be cooled to 2-8℃ within 3 minutes, to avoid blood clotting and other problems.

Description

Technical Field

[0001] This utility model is specifically an animal serum sample collection device with pre-cooling pipetting function, which relates to the field of blood testing technology. Background Technology

[0002] The paper "Effects of Poria cocos extract on serum biochemical indicators and lipid metabolism in broiler rabbits" indicates that dietary supplementation with 0.1-0.2% Poria cocos extract can significantly increase the content of serum protein and total protein in broiler rabbits, thereby improving their immunity and protein metabolism rate (see *China Feed*, 2024, No. 12). To verify the effects of Poria cocos extract, it is necessary to collect rabbit blood in batches and test the serum biochemical indicators of the rabbit blood.

[0003] See attached document Figure 1 In existing technology, serum is separated using a centrifuge. Before centrifugation, a small amount of gel is added to the blood collection tube 1, and then a rubber stopper 2 is placed on top. The specific gravity of the gel is greater than that of the serum but smaller than that of the blood cells; the function of the gel is to completely separate the separated serum from the blood cells. After centrifugation, a pale yellow serum layer 61 is located at the top of the blood collection tube, a colorless gel layer 62 is located in the middle of the blood collection tube, and a dark red blood cell layer 63 is located at the bottom of the blood collection tube.

[0004] See attached document Figure 2 After serum separation, a pipette 3 with a bulb syringe is used to aspirate the serum sample from blood collection tube 1 before performing multiple biochemical tests. The current problem is that to avoid aspirating the gel into the pipette, a portion of serum (approximately 10-15% of the total serum) always remains in the blood collection tube, which is a significant waste. Similarly, to obtain clean blood cells, some blood cells must be aspirated along with the gel. Rabbits are small and have a very limited blood volume. For a limited number of blood sample extractions, this blood loss is negligible, but for large-scale blood sample extractions, the blood loss is substantial, seriously affecting the health of the rabbits.

[0005] In addition, rabbit blood is collected in farms, where conditions are not available to cool it to 2-8°C within 5 minutes, which can easily lead to problems such as blood clotting. Utility Model Content

[0006] In order to overcome the shortcomings of the prior art, this utility model discloses an animal serum sample collection device with pre-cooling pipetting function, the purpose of which is to solve the problem of large blood sample separation loss in the prior art.

[0007] The present invention adopts the following technical solution:

[0008] An animal serum sample collection device with pre-cooling pipetting function, comprising:

[0009] Blood collection tubes;

[0010] pipette;

[0011] A cooling water jacket is fitted over the blood collection tube to cool the blood inside.

[0012] The transparent pipette plunger can be inserted into the blood collection tube in a sealed manner. The plunger has a through stepped hole, with a small, elongated hole at the bottom and a large hole at the top for inserting the pipette.

[0013] Further improvements to the technical solution: The cooling water jacket is mainly composed of a glass bottle and ice water contained inside the glass bottle.

[0014] Further improve the technical solution: Set a lower conical surface that is smaller at the top and larger at the bottom at the lower end of the small hole.

[0015] Further improve the technical solution: Set an upper conical surface that is larger at the top and smaller at the bottom between the small hole and the large hole.

[0016] Further improve the technical solution: A sealing ring is set on the outside of the pipette plunger, and the pipette plunger can be inserted into the blood collection tube in a sealed manner through the sealing ring.

[0017] Further technical improvements: A removable rubber stopper is installed on the blood collection tube.

[0018] After implementing the above technical solution, the beneficial effects of this utility model compared to the prior art are:

[0019] This novel pipette plunger features a stepped orifice that is wider at the top and narrower at the bottom. During serum separation, only about 1-3% of the total serum remains within the narrow orifice, significantly reducing serum sample loss. Furthermore, the pipette plunger can precisely remove the gel layer, yielding pure blood cells.

[0020] The cooling water jacket of this invention is easy to use and can cool blood collection to 2-8℃ within 3 minutes, avoiding problems such as blood clotting during blood collection. Attached Figure Description

[0021] Appendix Figure 1 The diagram shown illustrates the stratification of blood after centrifugation.

[0022] Appendix Figure 2 The diagram shown is a schematic of serum separation in the prior art.

[0023] Appendix Figure 3 The diagram shown is a schematic of the structure of this animal serum sample collection device.

[0024] Appendix Figure 4 The diagram shown is a schematic of the cooling water jacket.

[0025] Appendix Figure 5 The diagram shown is a schematic of the pipette plunger.

[0026] Appendix Figure 6-7 The diagram shown illustrates the process of separating serum samples using this animal serum sample collection device.

[0027] Appendix Figure 8 The diagram shown illustrates how the animal serum sample collection device separates blood cell samples.

[0028] In the attached diagram: 1. Blood collection tube; 2. Rubber stopper; 3. Pipette; 4. Cooling water jacket; 41. Glass bottle; 42. Ice water; 5. Pipette plunger; 51. Large orifice; 52. Small orifice; 53. Sealing ring; 54. Upper conical surface; 55. Lower conical surface; 61. Serum layer; 62. Gel layer; 63. Blood cell layer. Detailed Implementation

[0029] The preferred embodiments of this utility model are described below with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are only used to explain the technical principles of this utility model and are not intended to limit the scope of protection of this utility model. It should be noted that in the description of this utility model, terms such as "front," "rear," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. This is merely for ease of description and does not indicate or imply that the device or element must have a specific orientation and positional relationship, and therefore should not be construed as a limitation of this utility model. It should also be noted that in the description of this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, it can refer to a fixed connection, a detachable connection, or an integral connection; it can refer to a mechanical connection or an electrical connection; it can refer to a direct connection or an indirect connection through an intermediate medium, or a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0030] An animal serum sample collection device with pre-cooling pipetting function is disclosed, relating to the field of blood testing technology, primarily to address the problem of significant blood sample separation loss in existing technologies. The composition and working principle of this animal serum sample collection device are described in detail below.

[0031] See attached document Figure 3This animal serum sample collection device mainly consists of a blood collection tube 1, a pipette 3, a cooling water jacket 4, and a pipette plunger 5. The blood collection tube 1 and pipette 3 are existing products. The blood collection tube 1 is made of glass or transparent plastic, and the pipette 3 includes a pipette and a bulb syringe. A rubber stopper 2 is installed at the opening of the blood collection tube 1; the function of the rubber stopper 2 is to prevent blood from being ejected from the blood collection tube 1 during centrifugation.

[0032] See attached document Figure 4 , attached Figure 4 The diagram shows the structure of the cooling water jacket 4. The cooling water jacket 4 is fitted onto the blood collection tube 1 and is used to cool the blood inside the tube 1. In this embodiment, the cooling water jacket 4 mainly consists of a glass bottle 41 and ice water 42 contained within the glass bottle 41. Ice water 42 is readily available, and the glass bottle 41 facilitates observation of the melting process of the ice water 42. The ice water 42 has a temperature of 0°C and can cool the rabbit blood in the blood collection tube 1 to 2-8°C within 3 minutes, preventing problems such as blood clotting.

[0033] See attached document Figure 5 , attached Figure 5 The diagram shows the structure of the pipette plunger 5. The pipette plunger 5 is transparent and made of transparent materials such as glass or plastic. A stepped orifice is provided inside the pipette plunger 5; the lower small orifice 52 is elongated, and the upper large orifice 51 is coarser, allowing the pipette tube 3 to be inserted. An upper conical surface 54, wider at the top and narrower at the bottom, is provided between the small orifice 52 and the large orifice 51; the upper conical surface 54 helps reduce separation loss. A lower conical surface 55, narrower at the top and wider at the bottom, is provided at the lower end of the small orifice 52; the lower conical surface 55 helps the liquid to completely enter the small orifice. Furthermore, a sealing ring 53 is provided on the outside of the pipette plunger 5, allowing the pipette plunger 5 to be inserted into the blood collection tube 1 in a sealed manner.

[0034] Working principle:

[0035] See attached document Figure 6 When separating serum samples, insert the pipette plunger 5 into the blood collection tube 1. Since the pipette plunger 5 is transparent, the pale yellow serum layer 61 can be observed to enter the large hole 51 through the narrow hole 52. At this time, use the pipette 3 to aspirate the serum in the large hole 51.

[0036] See attached document Figure 7 As the pipette plunger 5 continues to descend, the colorless gel layer 62 also begins to enter the narrow orifice 52. To ensure the purity of the serum sample, the pipette 3 stops drawing serum at this point. Since the serum in the large orifice 51 is drawn away by the pipette 3, only a small amount of serum (approximately 1-3% of the total serum) remains in the narrow orifice 52, thus significantly reducing the separation loss of the serum sample.

[0037] See attached document Figure 8As the pipette stopper 5 continues to move downwards, the gel layer 62 completely enters the large well 51, and the dark red blood cell layer 63 also begins to enter the large well 51 from the small well 52. At this point, use the pipette 3 to aspirate the gel, and then pull out the pipette stopper 5. What remains in the blood collection tube 1 is a pure blood cell sample, which can be used directly for testing.

[0038] It is worth noting that the content not described in detail in the above embodiments is prior art. It is also worth noting that any additions, subtractions, substitutions, and improvements made by those skilled in the art based on the structure and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. An animal serum sample collection device with pre-cooling pipetting function, characterized in that: include: Blood collection tubes; pipette; A cooling water jacket is fitted over the blood collection tube to cool the blood inside. The transparent pipette plunger can be inserted into the blood collection tube in a sealed manner. The plunger has a through stepped hole, with a small, elongated hole at the bottom and a large hole at the top for inserting the pipette.

2. The animal serum sample collection device with pre-cooling pipetting function as described in claim 1, characterized in that: The cooling water jacket mainly consists of a glass bottle and ice water inside the glass bottle.

3. The animal serum sample collection device with pre-cooling pipetting function as described in claim 1, characterized in that: A lower conical surface, smaller at the top and larger at the bottom, is provided at the lower end of the small hole.

4. An animal serum sample collection device with pre-cooling pipetting function as described in claim 1 or 3, characterized in that: An upper conical surface, larger at the top and smaller at the bottom, is provided between the small hole and the large hole.

5. The animal serum sample collection device with pre-cooling pipetting function as described in claim 1, characterized in that: A sealing ring is provided on the outside of the pipette plunger, which allows the pipette plunger to be inserted into the blood collection tube in a sealed manner.

6. The animal serum sample collection device with pre-cooling pipetting function as described in claim 1, characterized in that: The blood collection tubes are fitted with removable rubber stoppers.

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