Chromatographic packing cleaning suction filter device

Abstract

The utility model discloses a chromatography filler cleaning suction filtration device, including mixing barrel, filtrate funnel, filtrate diaphragm and rotating clamping mechanism, mixing barrel is built -in mixing mechanism, and one side is provided with one -way liquid inlet pipe, is used for input reaction liquid or clean water to clean the chromatography filler in mixing barrel, filtrate funnel is detachably connected with mixing barrel, and the one end away from mixing barrel is connected with the suction filter pipe, is used for pumping the cleaning waste liquid after reaction, the filtrate diaphragm is clamped between mixing barrel and filtrate funnel, wherein, mixing barrel and filtrate diaphragm form mixing chamber between, and filtrate chamber is formed between filtrate funnel and filtrate diaphragm, rotating clamping mechanism is connected with the outer wall of mixing barrel, the utility model discloses the above -mentioned setting, to guarantee the premise of the effect of filling material cleaning, still can make in the cleaning process without needing to shift the filling material to this saves the time that consumes because of filling material shift, reaches simple operation and the purpose of promotion rate.

Description

Technical Field

[0001] This utility model relates to the field of biopharmaceuticals, and in particular to a chromatography packing cleaning and filtration device. Background Technology

[0002] In the pilot-scale process of filler development, the substrate spheres need to undergo key processes in sequence, including activation treatment, bonding reaction with protein solution, and separation and cleaning. Among these, after activation, residual activation solution must be removed to avoid interference from impurities, and after the bonding reaction, the filler spheres must also be thoroughly cleaned to remove unreacted protein solution, thus requiring multiple cleaning processes.

[0003] In existing packing cleaning processes, it is usually necessary to use components such as a three-necked flask with a mixing mechanism, a Buchner funnel, a filter flask, and a filtrate diaphragm to complete the cleaning. The specific cleaning process is as follows:

[0004] S1. Place the packing material and the reaction solution in a three-necked flask, and use a mixing mechanism to ensure that the packing material and the reaction solution react fully to obtain a solid-liquid mixture;

[0005] S2. Load the Buchner funnel onto the filter flask and place the solid-liquid mixture in the Buchner funnel. Solid-liquid separation is completed by using the filtrate diaphragm between the Buchner funnel and the filter flask.

[0006] S3. A filtrate tube connected to a vacuum filtration pump is connected to the outside of the filter bottle to provide a vacuum negative pressure force to the filter bottle, thereby accelerating solid-liquid separation and placing the pre-cleaned packing material at the bottom of the Buchner funnel and on the filtrate diaphragm.

[0007] S4. Place the pre-cleaned packing material back into the three-necked flask and add the reaction solution to obtain a solid-liquid mixture again.

[0008] Repeat steps S2 to S4 until cleaning is complete.

[0009] As can be seen from the above steps, during the cleaning process, the packing needs to be constantly transferred back and forth between the Buchner funnel and the three-necked flask, which is time-consuming and very inconvenient.

[0010] Therefore, a chromatography packing cleaning and filtration device is needed to solve the above problems. Utility Model Content

[0011] The purpose of this invention is to provide a chromatography packing cleaning and filtration device that, while ensuring the cleaning effect of the chromatography packing, eliminates the need to transfer the packing during the cleaning process, thereby saving the time spent on transferring the chromatography packing and achieving the goals of simple operation and increased speed.

[0012] To solve the above-mentioned technical problems, this utility model provides a chromatography packing cleaning and filtration device, including a mixing cylinder, a filtrate funnel, a filtrate diaphragm, and a rotating clamping mechanism.

[0013] The mixing cylinder has a built-in mixing mechanism and a one-way liquid inlet pipe on one side for inputting reaction liquid or water to clean the chromatography packing material inside the mixing cylinder.

[0014] The filtrate funnel is detachably connected to the mixing cylinder, and a suction pipe is connected to the end away from the mixing cylinder for suctioning the washing waste liquid after the reaction.

[0015] The filtrate diaphragm is sandwiched between the mixing cylinder and the filtrate funnel for separating the cleaning waste liquid from the chromatography packing material;

[0016] Wherein, a mixing chamber is formed between the mixing cylinder and the filtrate diaphragm, and a filtrate chamber is formed between the filtrate funnel and the filtrate diaphragm;

[0017] The rotating clamping mechanism is connected to the outer wall of the mixing cylinder and is used to control the mixing cylinder to rotate around a horizontal axis, so that the open end of the mixing cylinder can switch between vertically upward and vertically downward directions.

[0018] Furthermore, the filtrate funnel and the mixing cylinder are connected by a threaded connection.

[0019] Furthermore, the filtrate funnel has a connecting sleeve portion, and the inner wall of the connecting sleeve portion is provided with internal threads;

[0020] The outer wall of the mixing cylinder is provided with an external thread that matches the internal thread.

[0021] Furthermore, the filtrate diaphragm is sandwiched between the upper end face of the mixing cylinder and the stepped end face of the connecting sleeve.

[0022] Furthermore, the filtrate funnel and the suction tube are detachably connected by a connecting sleeve, and a vacuum suction pump is connected to the end of the suction tube away from the filtrate funnel.

[0023] Furthermore, the rotating clamping mechanism includes a stand, two detachably fixedly connected annular clamps, and a driving component;

[0024] The upright frame is located on one side of the mixing cylinder;

[0025] Two annular clamps are disposed on the outer wall of the mixing cylinder, and a clamping gap matching the outer wall of the mixing cylinder can be formed between the two annular clamps.

[0026] The drive unit is mounted on the upright and has an output shaft that is fixedly connected to the outer wall of one of the annular clamps.

[0027] Furthermore, the outer wall of the mixing cylinder is provided with an annular groove that matches the annular clamp.

[0028] Furthermore, both the one-way liquid inlet pipe and the suction filter pipe are configured as flexible hoses.

[0029] Compared with the prior art, the present invention has at least the following beneficial effects:

[0030] By configuring a mixing cylinder, a filtrate funnel, a filtrate diaphragm, and a rotating clamping mechanism, a mixing chamber is formed between the mixing cylinder and the filtrate diaphragm, and a filtrate chamber is formed between the filtrate funnel and the filtrate diaphragm. The filtrate funnel is connected to a suction filtration tube, and the mixing cylinder is connected to a one-way inlet pipe. This allows the chromatography packing material and the reaction solution to mix and react in the mixing chambers for reaction cleaning. After the reaction cleaning is complete, the rotating clamping mechanism causes the opening of the mixing cylinder to point vertically downwards, meaning the mixing cylinder and the filtrate funnel are inverted, thus allowing for further reaction cleaning. Under the action of force, the cleaning waste liquid can pass through the filtrate diaphragm and enter the filtrate funnel, and be discharged under the suction action of the suction tube. After it is completely discharged, the opening end of the mixing cylinder is made vertically upward by the rotating clamping mechanism, and the chromatography packing is repositioned on the bottom wall of the mixing cylinder under the action of gravity. The reaction solution is added again for cleaning to ensure the cleaning effect of the chromatography packing. Therefore, there is no need to transfer the chromatography packing multiple times during the entire cleaning process, thus saving the time spent on transferring the chromatography packing and achieving the purpose of simple operation and increased rate. Attached Figure Description

[0031] Figure 1 This is a cross-sectional view of the mixing cylinder with its opening facing vertically upwards in a chromatography packing cleaning and filtration device according to an embodiment of the present invention.

[0032] Figure 2 This is a cross-sectional view of the mixing cylinder with its opening facing downwards in a chromatography packing cleaning and filtration device according to an embodiment of the present invention.

[0033] Figure 3 This is a partial structural diagram of the mixing cylinder and the annular clamp in a chromatography packing cleaning and filtration device according to an embodiment of the present invention.

[0034] Reference numerals: 1. Mixing cylinder; 11. Mixing mechanism; 12. One-way liquid inlet pipe; 2. Filtrate funnel; 21. Connecting sleeve; 3. Filtrate diaphragm; 4. Rotary clamping mechanism; 41. Stand; 42. Annular clamp; 43. Drive component; 431. Output shaft; 5. Filter tube; 6. Mixing chamber; 7. Filtrate chamber; 8. Connecting sleeve. Detailed Implementation

[0035] The chromatography packing cleaning and filtration device of this utility model will now be described in more detail with reference to the schematic diagrams, which illustrate preferred embodiments of this utility model. It should be understood that those skilled in the art can modify the utility model described herein while still achieving its advantageous effects. Therefore, the following description should be understood as being of general knowledge to those skilled in the art and is not intended to limit the utility model.

[0036] Furthermore, based on the teachings of this specification, those skilled in the art can form new technical solutions through cross-combination of different implementation methods without creating technical contradictions. Such variations should all be considered to fall within the protection scope of this patent.

[0037] The present invention will be described in more detail below by way of example with reference to the accompanying drawings. The advantages and features of the present invention will become clearer from the following description. It should be noted that the drawings are in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of the present invention.

[0038] like Figures 1 to 3 As shown in the figure, this utility model embodiment proposes a chromatography packing cleaning and filtration device, including a mixing cylinder 1, a filtrate funnel 2, a filtrate diaphragm 3, and a rotating clamping mechanism 4.

[0039] The mixing cylinder 1 has a built-in mixing mechanism 11 and a one-way liquid inlet pipe 12 on one side for inputting reaction liquid or clean water to clean the chromatography packing material inside the mixing cylinder 1. That is, by inputting reaction liquid or clean water, the chromatography packing material is subjected to reaction cleaning or water rinsing.

[0040] The filtrate funnel 2 is detachably connected to the mixing cylinder 1, and a suction pipe 5 is connected to the end away from the mixing cylinder 1 for suctioning the cleaning waste liquid after the reaction. That is, after the chromatography packing material and the reaction solution are mixed and reacted, the resulting cleaning waste liquid can be discharged by means of the suction pipe 5, so as to facilitate the re-injection of the subsequent reaction solution for secondary or multiple cleaning.

[0041] The filtrate diaphragm 3 is sandwiched between the mixing cylinder 1 and the filtrate funnel 2, and is used to separate the cleaning waste liquid from the chromatography packing material. That is, by setting the filtrate diaphragm 3, only the liquid can pass through the filtrate diaphragm 3 to enter the filtrate funnel 2 from the mixing cylinder 1, while the chromatography packing material is intercepted by the filtrate diaphragm 3, so as to achieve the function of solid-liquid separation.

[0042] The mixing chamber 6 is formed between the mixing cylinder 1 and the filtrate diaphragm 3, and the filtrate funnel 2 is formed between the filtrate diaphragm 3. The mixing chamber 6 is used for mixing the chromatography packing material and the reaction solution, while the filtrate chamber 7 is used for discharging the washing waste liquid.

[0043] Furthermore, the rotating clamping mechanism 4 is connected to the outer wall of the mixing cylinder 1 and is used to control the rotation of the mixing cylinder 1 around a horizontal axis, so that the open end of the mixing cylinder 1 switches between vertically upward and vertically downward directions. Through the operation of the rotating clamping mechanism 4, the relative position between the mixing cylinder 1 and the filtrate funnel 2 can change, such as the mixing cylinder 1 changing from an upright state (e.g., ...). Figure 1 (as shown) becomes an inverted state (as shown) Figure 2 (As shown), to be used for mixing the chromatography packing material and the reaction solution or for discharging the cleaning waste liquid.

[0044] This device comprises a mixing cylinder 1, a filtrate funnel 2, a filtrate diaphragm 3, and a rotating clamping mechanism 4. A mixing chamber 6 is formed between the mixing cylinder 1 and the filtrate diaphragm 3, and a filtrate chamber 7 is formed between the filtrate funnel 2 and the filtrate diaphragm 3. The filtrate funnel 2 is connected to a suction filtration tube 5, and the mixing cylinder 1 is connected to a one-way inlet pipe 12. This allows the chromatography packing material (such as…) to… Figure 1 The circled area indicates the reaction solution (as shown in the image) and the reaction solution (e.g. Figure 1 As indicated by the middle arrow, the mixture can be mixed and reacted in the mixing chamber 6 for reaction cleaning. After the reaction cleaning is completed, under the action of the rotating clamping mechanism 4, the opening end of the mixing cylinder 1 is made to face vertically downward, that is, the mixing cylinder 1 and the filtrate funnel 2 are inverted in opposite directions. Then, under the action of gravity, the cleaning waste liquid (such as...) is removed. Figure 2 (As indicated by the middle arrow) It can pass through the filtrate diaphragm 3 and enter the filtrate funnel 2, and be discharged under the suction of the suction tube 5. After it is completely discharged, the opening end of the mixing cylinder 1 is made to face vertically upward by the rotating clamping mechanism 4. The chromatography packing is repositioned on the bottom wall of the mixing cylinder 1 under the action of gravity. The reaction solution is added again for cleaning to ensure the cleaning effect of the chromatography packing. Therefore, the chromatography packing does not need to be transferred multiple times during the entire cleaning process, thus saving the time spent on transferring the chromatography packing and achieving the purpose of simple operation and increased rate.

[0045] In other embodiments, to accelerate the separation of the chromatography packing material from the filtrate membrane 3, the mixing cylinder 1 can be controlled to reciprocate within a certain deflection angle (i.e., not exceeding 180°) by the rotating clamping mechanism 4. Under the action of oscillation, the chromatography packing material and the filtrate membrane 3 can be separated more quickly, avoiding the situation where the chromatography packing material cannot react and be cleaned with the reaction solution due to adhesion to the filtrate membrane 3 during secondary or multiple cleaning.

[0046] In this embodiment, in order to ensure the sealing of the connection between the filtrate funnel 2 and the mixing cylinder 1, and to prevent external impurities from entering the mixing cylinder 1 and contaminating the chromatography packing, the connection between the two is further defined. Specifically, the filtrate funnel 2 and the mixing cylinder 1 are connected by threads.

[0047] In this embodiment, the filtrate funnel 2 has a connecting sleeve portion 21, the inner wall of the connecting sleeve portion 21 is provided with an internal thread, and the outer wall of the mixing cylinder 1 is provided with an external thread that matches the internal thread, so that the outer diameter of the filtrate funnel 2 is larger than the outer diameter of the mixing cylinder 1, thereby facilitating the clamping of the filtrate diaphragm 3 between the two.

[0048] Specifically, the filtrate diaphragm 3 is sandwiched between the upper end face of the mixing cylinder 1 and the stepped end face of the connecting sleeve portion 21.

[0049] In other embodiments, the filtrate funnel 2 and the suction tube 5 are detachably connected by a connecting sleeve 8 to facilitate maintenance and replacement.

[0050] In a specific example, the connecting sleeve 8 is configured as a pipe connection with internal threads.

[0051] The end of the filtration tube 5 away from the filtrate funnel 2 is connected to a vacuum filtration pump, which provides suction force for the cleaning waste liquid.

[0052] In a further embodiment, a specific rotating clamping mechanism 4 is proposed to better control the rotation of the mixing cylinder 1.

[0053] Specifically, the rotating clamping mechanism 4 includes a stand 41, two detachably fixed ring clamps 42, and a driving component 43.

[0054] The support frame 41 is located on one side of the mixing cylinder 1 for support.

[0055] Two annular clamps 42 are disposed on the outer wall of the mixing cylinder 1, and the two annular clamps 42 can form a clamping gap that matches the outer wall of the mixing cylinder 1. That is, by changing the state of the two annular clamps 42, they can be kept relatively fixed with the mixing cylinder 1. Thus, by driving the annular clamps 42 to rotate, the mixing cylinder 1 can be rotated.

[0056] In this embodiment, the drive member 43 is disposed on the upright frame 41 and has an output shaft 431 fixedly connected to the outer wall of one of the annular clamps 42.

[0057] To improve the stability of the mixing cylinder 1 during rotation, an annular groove matching the annular clamp 42 is provided on the outer wall of the mixing cylinder 1.

[0058] To prevent interference between the one-way inlet pipe 12 and the suction filter pipe 5 and the upright frame 41 during the rotation of the mixing cylinder 1, both the one-way inlet pipe 12 and the suction filter pipe 5 are made into flexible hoses.

[0059] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. A chromatographic packing washing and suction filtration apparatus, characterized by, It includes a mixing cylinder (1), a filtrate funnel (2), a filtrate diaphragm (3), and a rotary clamping mechanism (4); The mixing cylinder (1) has a built-in mixing mechanism (11) and a one-way liquid inlet pipe (12) on one side for inputting reaction liquid or water to clean the chromatography packing material inside the mixing cylinder (1). The filtrate funnel (2) is detachably connected to the mixing cylinder (1), and a suction pipe (5) is connected to the end away from the mixing cylinder (1) for suctioning the cleaning waste liquid after the reaction. The filtrate diaphragm (3) is sandwiched between the mixing cylinder (1) and the filtrate funnel (2) for separating the cleaning waste liquid from the chromatography packing material; The mixing cylinder (1) and the filtrate diaphragm (3) form a mixing chamber (6), and the filtrate funnel (2) and the filtrate diaphragm (3) form a filtrate chamber (7). The rotating clamping mechanism (4) is connected to the outer wall of the mixing cylinder (1) and is used to control the mixing cylinder (1) to rotate around the horizontal axis, so that the opening end of the mixing cylinder (1) switches between vertically upward and vertically downward directions.

2. A chromatography media washing and filtering apparatus as claimed in claim 1, wherein, The filtrate funnel (2) and the mixing cylinder (1) are connected by threads.

3. A chromatography media washing and filtering apparatus as claimed in claim 2, wherein, The filtrate funnel (2) has a connecting sleeve (21), and the inner wall of the connecting sleeve (21) is provided with an internal thread; The outer wall of the mixing cylinder (1) is provided with an external thread that matches the internal thread.

4. A chromatography media washing and filtering apparatus as claimed in claim 3, wherein, The filtrate diaphragm (3) is sandwiched between the upper end face of the mixing cylinder (1) and the stepped end face of the connecting sleeve (21).

5. The chromatography media washing and filtering apparatus of claim 1, wherein, The filtrate funnel (2) and the suction tube (5) are detachably connected by a connecting sleeve (8), and a vacuum suction pump is connected to the end of the suction tube (5) away from the filtrate funnel (2).

6. The chromatography packing cleaning and filtration device as described in claim 1, characterized in that, The rotating clamping mechanism (4) includes a stand (41), two detachably fixed ring clamps (42), and a drive component (43); The upright frame (41) is located on one side of the mixing cylinder (1); Two annular clamps (42) are disposed on the outer wall of the mixing cylinder (1), and a clamping gap matching the outer wall of the mixing cylinder (1) can be formed between the two annular clamps (42); The drive unit (43) is mounted on the stand (41) and has an output shaft (431) that is fixedly connected to the outer wall of one of the annular clamps (42).

7. The chromatography packing cleaning and filtration device as described in claim 6, characterized in that, The outer wall of the mixing cylinder (1) is provided with an annular groove that matches the annular clamp (42).

8. The chromatography packing cleaning and filtration device as described in claim 1, characterized in that, Both the one-way liquid inlet pipe (12) and the suction filter pipe (5) are configured as flexible hoses.

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