An mRNA in vitro transcription kit

Abstract

The utility model relates to the technical fields of kit, put forward a kind of mRNA in vitro transcription kit, kit inside is separated into storage cavity and refrigeration cavity by partition, the refrigeration cavity inside is equipped with refrigeration device, the storage cavity inside includes the support seat fixed in the upper portion of partition, rotatable connection has the rotating shaft of vertical arrangement on the support seat, the top of rotating shaft coaxially is fixed with the rack for placing reagent tube for placing, driving hole is opened along the axis in the rack, the driving hole passes through rotating shaft and extends to the inside of support seat, spiral rod is passed in the inside of driving hole, the driving hole is located on the inner wall of rack and is opened with the spiral groove of the appearance matching of spiral rod. The utility model has the beneficial effects that: using spiral rod and refrigeration device, RNA polymerase can be centrifuged in kit, wait for use, do not need to take reagent out of box again, reduce the risk of temperature rise enzyme activity decline.

Description

Technical Field

[0001] This invention relates to the technical field of reagent kits, specifically to an mRNA in vitro transcription kit. Background Technology

[0002] Most reagents used for in vitro mRNA transcription require cryopreservation. RNA polymerase reagent, in particular, needs to be stored at -20°C, typically in a freezer. Before use, a brief centrifugation is performed to remove condensate from the tube walls or caps, mix any precipitates or separated components, avoid air bubbles interfering with the accuracy of liquid collection, and remove any possible protein aggregates. After centrifugation, the reagent is placed on an ice box to maintain a low-temperature environment, awaiting the preparation of the entire reaction system with other reagents according to their components and dosages. The entire process requires multiple pieces of equipment, including freezers, centrifuges, and ice boxes, making the operation complex. Furthermore, the fact that centrifugation is performed at room temperature may lead to a decrease in enzyme activity and damage to reagent stability. In addition, the centrifugation time cannot be precisely consistent each time, resulting in poor reproducibility of the experiment. Utility Model Content

[0003] This invention proposes an in vitro mRNA transcription kit that utilizes a spiral rod and a cooling device to allow RNA polymerase to undergo centrifugation within the kit, eliminating the need to remove the kit from the container. This reduces the risk of decreased enzyme activity due to temperature rise, saves experimental time and steps, and improves experimental reproducibility.

[0004] Therefore, the technical solution adopted is as follows:

[0005] An mRNA in vitro transcription kit includes a housing. The housing is internally divided into a storage chamber and a cooling chamber by an isolation plate. The cooling chamber contains a cooling device. The storage chamber includes a support fixed above the isolation plate. A vertically arranged rotating shaft is rotatably connected to the support. A holder for placing reagent tubes is coaxially fixed to the top of the rotating shaft. A driving hole is formed along the central axis of the holder. The driving hole passes through the rotating shaft and extends into the support. A helical rod passes through the driving hole. A helical groove matching the shape of the helical rod is formed on the inner wall of the holder.

[0006] A further technical solution is that a snap-fit ​​block is fixed at the top of the spiral rod, and a snap-fit ​​groove matching the snap-fit ​​block is provided on the placement frame, so that the snap-fit ​​block can be embedded inside the snap-fit ​​groove.

[0007] A further technical solution is that the placement rack has several evenly distributed placement holes, and two support grooves are symmetrically opened on both sides of the placement holes. A support frame is inserted into the placement hole. The support frame is a cylindrical structure with an inner diameter matching the outer diameter of the reagent tube. Two support rods are symmetrically fixed on both sides of the support frame, and the two support rods can be correspondingly inserted into the two support grooves.

[0008] A further technical solution is that the bottom of the support groove is provided with a vertical groove and an inclined groove, and the bottom of the support rod is fixed with an insertion block, which matches both the vertical groove and the inclined groove.

[0009] A further technical solution is that the upper and lower edges of the placement hole are provided with bevels to accommodate the reagent tube being placed at an angle.

[0010] A further technical solution is that a number of placement holes are evenly distributed around the central axis of the placement frame in layers, and the diameter of the placement holes in each layer is different.

[0011] A further technical solution is that a support column is fixed to the bottom of the isolation plate, and the support column is fixed to the box body.

[0012] The working principle and beneficial effects of this application are as follows:

[0013] 1. The centrifugation of reagents on the rack is achieved by quickly pulling out the screw rod to drive the rack to rotate at high speed. At the same time, the cooling device allows RNA polymerase to be provided in the kit for centrifugation, ready for use, without having to take the reagent out of the kit. This reduces the risk of enzyme activity decreasing due to temperature rise, saves experimental time and steps, and improves the reproducibility of the experiment.

[0014] 2. During the storage of reagent tubes, the inserter is inserted into the vertical groove to keep the tubes upright for easy liquid retrieval. During centrifugation, the inserter is inserted into the horizontal groove to keep the reagent tubes tilted, thereby increasing the sedimentation path of the reagents during centrifugation, improving centrifugation efficiency, reducing convection interference, and stabilizing stratification. Attached Figure Description

[0015] The present application will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0016] Figure 1 This is a schematic diagram of the overall internal structure of this application;

[0017] Figure 2 This is a schematic diagram of the structure of the placement rack described in this application;

[0018] Figure 3 This is a schematic diagram of the support frame described in this application placed vertically;

[0019] Figure 4 This is a schematic diagram of the support frame described in this application, placed at an angle.

[0020] Figure 5 This is a schematic diagram of the support groove described in this application;

[0021] Figure 6 This is a schematic diagram of the structure of the screw rod described in this application;

[0022] Figure 7 This is a cross-sectional structural diagram of the support base described in this application;

[0023] Figure 8 This is a schematic diagram of the support frame described in this application.

[0024] In the diagram: 100, box body; 10, isolation plate; 200, reagent tube; 1, storage chamber; 11, support base; 12, rotating shaft; 2, refrigeration chamber; 21, refrigeration device; 3, placement rack; 31, drive hole; 32, spiral rod; 321, snap-fit ​​block; 33, spiral groove; 34, snap-fit ​​groove; 35, placement hole; 36, support groove; 361, vertical groove; 362, inclined groove; 37, support frame; 371, support rod; 372, insertion block; 351, bevel; 4, support column. Detailed Implementation

[0025] The technical solutions of this utility model will be clearly and completely described below with reference to the embodiments of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this utility model.

[0026] like Figures 1-8 As shown, an mRNA in vitro transcription kit includes a box 100. The box 100 is divided into a storage chamber 1 and a cooling chamber 2 by an isolation plate 10. The cooling chamber 2 has a cooling device 21 inside. The storage chamber 1 includes a support base 11 fixed above the isolation plate 10. A vertically arranged rotating shaft 12 is rotatably connected to the support base 11. A placement rack 3 for placing reagent tubes 200 is coaxially fixed at the top end of the rotating shaft 12. A driving hole 31 is opened along the central axis of the placement rack 3. The driving hole 31 passes through the rotating shaft 12 and extends into the support base 11. A spiral rod 32 passes through the driving hole 31. A spiral groove 33 matching the shape of the spiral rod 32 is opened on the inner wall of the placement rack 3.

[0027] The refrigeration device 21 used in this embodiment can be rechargeable for short-term storage, while for long-term transport it needs to be placed in a refrigerated transport vehicle or connected to an external power source. In special cases, ice packs or other low-temperature substances can also be used to achieve a cooling effect. This kit can simultaneously perform transport, storage, centrifugation, and liquid retrieval functions, and can be used in appropriate steps as needed. Generally, before starting the experiment, the required dose of RNA polymerase reagent can be directly placed into the kit for later use. Because the kit is still in a low-temperature environment, the activity and stability of the enzyme will not be affected.

[0028] Before the transcription process begins, the helical rod 32 is quickly pulled outward. Since the driving hole 31 is located on the inner wall of the placement rack 3 and has a helical groove 33 that matches the shape of the helical rod 32, as the helical rod 32 is pulled out, the entire placement rack 3 will rotate rapidly around the rotation axis 12, thereby driving the reagent tube 200 above it to perform centrifugation, removing the condensate on the tube wall or cap, and mixing the precipitate or layered components. Since the RNA polymerase reagent only requires simple centrifugation to meet the needs, after pulling out the helical rod 32, other reagents can be prepared and configured without any further operations. The placement rack 3 will automatically stop afterward. The RNA polymerase reagent after separation will remain on the placement rack 3 waiting for liquid collection. The whole process is simpler and more efficient, without the need for transfer from the centrifuge to the ice box.

[0029] In subsequent processes, the screw rod 32 can be slowly inserted back at any time to prevent loss or damage. Figure 2 As shown, a snap-fit ​​block 321 is fixed to the top of the spiral rod 32. The placement frame 3 has a snap-fit ​​groove 34 that matches the snap-fit ​​block 321. The snap-fit ​​block 321 can be embedded in the snap-fit ​​groove 34, so that the spiral rod 32 can be better stored when it is not needed. A handheld gripping device can also be fixed to the top of the snap-fit ​​block 321 to facilitate pulling out and inserting the spiral rod 32.

[0030] The placement frame 3 has several evenly distributed placement holes 35. Two support grooves 36 are symmetrically opened on both sides of each placement hole 35. A support frame 37 is inserted into the placement hole 35. The support frame 37 is a cylindrical structure with an inner diameter matching the outer diameter of the reagent tube 200. Two support rods 371 are symmetrically fixed on both sides of the support frame 37. The two support rods 371 can be inserted into the two support grooves 36 respectively. The support frame 37 is secured in the placement hole 35 by the support rods 371. When in use, the reagent tube 200 is inserted into the support frame 37.

[0031] like Figures 2-5As shown, the bottom of the support groove 36 has a vertical groove 361 and an inclined groove 362. An insertion block 372 is fixed to the bottom of the support rod 371. The insertion block 372 matches both the vertical groove 361 and the inclined groove 362. The inclined groove 362 has an inclination angle of 30° to 45° relative to the vertical groove 361. During the storage of the reagent tube 200, the insertion block 372 is inserted into the vertical groove 361 to keep it vertical, facilitating liquid retrieval. During centrifugation, the insertion block 372 is inserted into the inclined groove 362, causing the support frame 37 to tilt the reagent tube 200, thereby increasing the sedimentation path during centrifugation, improving centrifugation efficiency, reducing convection interference, and stabilizing stratification. Furthermore, to accommodate the tilting of the support frame 37, bevels 351 are provided at the upper and lower edges of the placement hole 35 to accommodate the inclined placement of the reagent tube 200.

[0032] like Figure 2 As shown, several placement holes 35 are arranged in an even number and distributed layer by layer around the central axis of the placement rack 3. The diameter of each layer of placement holes 35 is different. The even number of placement holes 35 ensures that there are always two placement holes 35 that are symmetrical. Thus, during centrifugation, the corresponding reagent tubes 200 can be placed symmetrically, making the centrifugal force more stable and balanced. The different diameters of each layer of placement holes 35 correspond to a wider variety of reagent tube diameters 200, meeting more experimental needs.

[0033] Because the isolation plate 10 is the main stress-bearing component in the entire kit, therefore... Figure 1 As shown, a support column 4 is fixed at the bottom of the isolation plate 10. The support column 4 is fixed on the box body 100 and supports the isolation plate 10 to ensure the structural stability of the entire reagent kit.

[0034] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. An mRNA in vitro transcription kit comprising a cartridge (100), characterized in that, The box body (100) is divided into a storage chamber (1) and a cooling chamber (2) by an isolation plate (10). The cooling chamber (2) has a cooling device (21). The storage chamber (1) includes a support base (11) fixed above the isolation plate (10). A vertically arranged rotating shaft (12) is rotatably connected to the support base (11). A placement rack (3) for placing reagent tubes (200) is coaxially fixed at the top of the rotating shaft (12). A driving hole (31) is opened along the central axis of the placement rack (3). The driving hole (31) passes through the rotating shaft (12) and extends into the support base (11). A spiral rod (32) passes through the driving hole (31). A spiral groove (33) matching the shape of the spiral rod (32) is opened on the inner wall of the placement rack (3) of the driving hole (31).

2. The mRNA in vitro transcription kit according to claim 1, characterized in that, The top end of the spiral rod (32) is fixed with a snap-fit ​​block (321), and the placement frame (3) is provided with a snap-fit ​​groove (34) that matches the snap-fit ​​block (321). The snap-fit ​​block (321) can be embedded inside the snap-fit ​​groove (34).

3. The mRNA in vitro transcription kit according to claim 1, characterized in that, The placement rack (3) has several evenly distributed placement holes (35). Two support grooves (36) are symmetrically opened on both sides of the placement holes (35). A support frame (37) is inserted into the placement hole (35). The support frame (37) is a cylindrical structure with an inner diameter matching the outer diameter of the reagent tube (200). Two support rods (371) are symmetrically fixed on both sides of the support frame (37). The two support rods (371) can be inserted into the two support grooves (36) respectively.

4. The mRNA in vitro transcription kit according to claim 3, characterized in that, The bottom of the support groove (36) is provided with a vertical groove (361) and an inclined groove (362), and the bottom of the support rod (371) is fixed with an insertion block (372), which matches both the vertical groove (361) and the inclined groove (362).

5. The mRNA in vitro transcription kit according to claim 4, characterized in that, The placement hole (35) has bevels (351) at its upper and lower edges to accommodate the reagent tube (200) placed at an angle.

6. The mRNA in vitro transcription kit according to claim 1, characterized in that, Several placement holes (35) are arranged in an even number and are distributed layer by layer around the central axis of the placement frame (3), and the diameter of each layer of placement holes (35) is different.

7. The mRNA in vitro transcription kit according to claim 1, characterized in that, The bottom of the isolation plate (10) is fixed with a support column (4), which is fixed on the box body (100).

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