A collection device for sub-totipotent stem cells

Abstract

The utility model relates to stem cell collection technical field, concretely disclose a kind of collection device for subtotipotent stem cell, including filter cartridge, the bottom of filter cartridge is fixedly installed with closure, the top of filter cartridge is fixedly installed with input pipe, the bottom of filter cartridge is fixedly installed with discharge pipe, the outside of discharge pipe is provided with sterile collection bottle, the inside of filter cartridge is provided with filter assembly, the top of input pipe is provided with fixed dismounting assembly, the top of fixed dismounting assembly is provided with connecting pipe, the top of connecting pipe is fixedly installed with collection bag, this is used for the collection device of subtotipotent stem cell, flange bolt connection and pipe clamp sealing design are used in fixed dismounting assembly, both through rigid connection ensure pipeline stable, and sterile environment is formed using sealing washer to prevent pollution, and support quick dismounting replacement collection bag, and give consideration to sealing property, convenience and reuse requirement.

Description

Technical Field

[0001] This utility model relates to the field of stem cell collection technology, specifically to a collection device for sub-pluripotent stem cells. Background Technology

[0002] In the biomedical field, subpluripotent stem cells have shown important application value in regenerative medicine, cell therapy and other fields due to their multipotent differentiation potential and immune regulation function. The efficient collection of subpluripotent stem cells is a key link in subsequent research and clinical application, and the core technical performance of the collection device directly affects the quality and efficiency of stem cell acquisition.

[0003] In the collection of subpluripotent stem cells, a bone marrow aspiration needle is used to puncture the bone marrow cavity, extract bone marrow fluid, and inject it into a collection bag containing an anticoagulant to prevent clotting. Immediately after collection, the collection bag is gently inverted to allow the anticoagulant to mix thoroughly with the bone marrow fluid, preventing the formation of blood clots. Then, the bone marrow sample (containing an anticoagulant, such as heparin or EDTA) is poured into a filter. The liquid components (plasma, blood cell suspension) pass through the filter under gravity and enter a sterile collection bottle. Large particulate impurities are retained. However, since bone marrow is composed of hematopoietic tissue, adipose tissue, bone trabecular fragments, and vascular connective tissue, a large number of blood cells (red blood cells, white blood cells, platelets), fat droplets, blood clots, and connective tissue debris will be mixed in during collection (such as iliac crest puncture or bone marrow blood collection). These cell debris can clog the filter during the collection process, thus affecting the collection efficiency. Therefore, we propose a collection device for subpluripotent stem cells. Utility Model Content

[0004] The purpose of this invention is to provide a collection device for subpluripotent stem cells, in order to solve the problem mentioned in the background art that during collection (such as iliac crest puncture or bone marrow blood collection), a large number of blood cells (red blood cells, white blood cells, platelets), fat droplets, blood clots and connective tissue debris are mixed in, causing these cell debris to clog the filter screen during the collection process, thereby affecting the collection efficiency.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a collection device for sub-pluripotent stem cells, wherein a sealing cap is fixedly installed at the bottom of a filter cylinder, an input tube is fixedly installed at the top of the filter cylinder, an output tube is fixedly installed at the bottom of the filter cylinder, a sterile collection bottle is provided outside the output tube, a filter assembly is provided inside the filter cylinder, a fixing and disassembly assembly is provided at the top of the input tube, a connecting tube is provided at the top of the fixing and disassembly assembly, and a collection bag is fixedly installed at the top of the connecting tube.

[0006] The fixed disassembly assembly includes a second flange, which is fixedly installed on the top of the inlet pipe. The second flange is threaded with bolts through its internal threaded holes. The bolts are externally threaded to the first flange. The second flange contacts and merges with the first flange. The second flange is fixed to the first flange by bolts.

[0007] The second flange and the first flange are fitted with a pipe clamp on the outside. The pipe clamp wraps around the merged second flange and the first flange. A sealing ring is fixedly installed inside the pipe clamp. The sealing ring covers the gap left after the second flange and the first flange are merged. An output pipe is fixedly installed on the top of the first flange. A connecting pipe is fixedly installed on the top of the output pipe.

[0008] The filter assembly includes a micro motor, which is fixedly installed at the bottom of the filter cartridge. A second intermittent gear is fixedly installed at one end of the output shaft of the micro motor. A connecting rod is fixedly installed at the top of the second intermittent gear. A first intermittent gear is fixedly installed at the top of the connecting rod. A driven gear is rotatably connected inside the filter cartridge.

[0009] The first intermittent gear is meshed with the driven gear. A rotating disk is fixedly installed on the top of the driven gear. Multiple cell filters are fixedly installed on the rotating disk through the through holes inside it. Multiple installation tubes are fixedly installed on the rotating disk through the through holes inside it. The rotating disk, driven gear, installation tubes and cell filters are all symmetrically arranged in twos about the transverse central axis of the connecting rod.

[0010] One of the driven gears is not meshed with the second intermittent gear. A conveying pipe is fixedly installed inside the filter cylinder. The bottom of the input pipe and the top of the conveying pipe are in contact with the cell filter inside the rotating disk. The bottom of the conveying pipe and the top of the discharge pipe are in contact with the mounting pipe inside another rotating disk.

[0011] This utility model has at least the following beneficial effects:

[0012] The fixed and disassembly assembly employs a flange bolt connection and pipe clamp sealing design, which ensures pipeline stability through rigid connection and creates a sterile environment to prevent contamination using sealing rings. It also supports quick disassembly and replacement of collection bags, taking into account the requirements of sealing, convenience and reusability. The filtration assembly is based on a gear intermittent transmission structure driven by a micro motor. Two sets of rotating disks alternately perform filtration and diversion functions. It can achieve precise separation by intercepting non-target cells through the filter screen, and automatically switch working states when the filtration rate decreases to avoid clogging and ensure continuous sample processing, significantly improving collection efficiency and equipment stability. Attached Figure Description

[0013] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0014] Figure 2 This is a schematic diagram of the exploded three-dimensional structure of this utility model;

[0015] Figure 3 This is an exploded three-dimensional structural diagram of the fixing and disassembly assembly of this utility model;

[0016] Figure 4 This is an exploded three-dimensional structural diagram of the filter component of this utility model.

[0017] In the diagram: 1. Sealing cap; 2. Filter cartridge; 3. Sterile collection bottle; 4. Collection bag; 5. Fixing and disassembly assembly; 51. Output tube; 52. Bolt; 53. First flange; 54. Second flange; 55. Pipe clamp; 56. Sealing ring; 6. Input tube; 7. Filter assembly; 71. Rotating disc; 72. Cell filter; 73. Installation tube; 74. Delivery tube; 75. Driven gear; 76. First intermittent gear; 77. Connecting rod; 78. Second intermittent gear; 79. Micro motor; 8. Discharge tube; 9. Connecting tube. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figures 1 to 4 This utility model provides a technical solution: a collection device for sub-pluripotent stem cells, including a filter cylinder 2, a sealing cap 1 fixedly installed on one side of the filter cylinder 2, an input pipe 6 fixedly installed on the top of the filter cylinder 2, an output pipe 8 fixedly installed on the bottom of the filter cylinder 2, a sterile collection bottle 3 provided outside the output pipe 8, a filter assembly 7 provided inside the filter cylinder 2, a fixing and disassembly assembly 5 provided on the top of the input pipe 6, a connecting pipe 9 provided on the top of the fixing and disassembly assembly 5, and a collection bag 4 fixedly installed on the top of the connecting pipe 9.

[0020] During the collection of sub-pluripotent stem cells, the collected bone marrow sample is placed in the collection bag 4. After the device starts working, the upper rotating disk 71 is in the filtering state. The bone marrow sample enters the cell filter 72 through the input tube 6 for filtration. Non-stem cell components are retained, and the sub-pluripotent stem cells flow with the filtrate through the delivery tube 74, the installation tube 73, and the discharge tube 8 into the sterile collection bottle 3. After the upper cell filter 72 has been used for a period of time, the micro motor 79 drives the rotating disk 71 to switch states. The lower rotating disk 71 enters the filtering state, and the upper rotating disk 71 enters the flow guiding state, realizing continuous filtration.

[0021] The fixed disassembly assembly 5 includes a second flange 54, which is fixedly installed on the top of the inlet pipe 6. A bolt 52 is threadedly connected to the second flange 54 through a threaded hole inside it. A first flange 53 is threadedly connected to the bolt 52. The second flange 54 and the first flange 53 contact and merge. The second flange 54 is fixed to the first flange 53 by the bolt 52. A pipe clamp 55 is provided outside the second flange 54 and the first flange 53, enclosing the merged second flange 54 and the first flange 53. A sealing ring 56 is fixedly installed inside the pipe clamp 55, covering the gap left after the second flange 54 and the first flange 53 are merged. An output pipe 51 is fixedly installed at the top, and a connecting pipe 9 is fixedly installed at the top of the output pipe 51. The connecting pipe 9 is made of medical-grade silicone tubing, which has good elasticity and aging resistance and can be bent and adjusted according to actual operation requirements. The second flange 54 is fixed to the top of the input pipe 6 and is threadedly connected to the first flange 53 by bolts 52, so as to achieve a rigid connection between the output pipe 51 and the input pipe 6 of the collection bag 4. The pipe clamp 55 wraps around the merged second flange 54 and first flange 53, and the internal sealing ring 56 fills the gap to form a sterile sealed environment to prevent external contamination from entering the filter cartridge 2. When replacing the collection bag 4, the second flange 54 and first flange 53 can be separated by loosening the bolts 52 and removing the pipe clamp 55, which meets the requirements of quick replacement and reuse.

[0022] The filter assembly 7 includes a micro motor 79, which is fixedly installed at the bottom of the filter cartridge 2. A second intermittent gear 78 is fixedly installed at one end of the output shaft of the micro motor 79. A connecting rod 77 is fixedly installed at the top of the second intermittent gear 78, and a first intermittent gear 76 is fixedly installed at the top of the connecting rod 77. A driven gear 75 is rotatably connected inside the filter cartridge 2. The first intermittent gear 76 and the driven gear 75 are meshed together. A rotating disk 71 is fixedly installed at the top of the driven gear 75. Multiple cell filters 72 are fixedly installed on the rotating disk 71 through through holes in its interior. Multiple mounting tubes 73 are fixedly installed on the rotating disk 71 through through holes in its interior. The multiple cell filters 72 and the mounting tubes 73 are spaced apart, and the upper and lower rotating disks 71 always keep the cell filters 72 and the mounting tubes 73 in the same vertical plane. The rotating disk 71, the driven gear 75, the mounting tubes 73 and the cell filters 72 are all symmetrical about the transverse central axis of the connecting rod 77. There are two sets of rotating discs. The other driven gear 75 is not engaged with the second intermittent gear 78. A delivery pipe 74 is fixedly installed inside the filter cylinder 2. The bottom of the input pipe 6 and the top of the delivery pipe 74 are in contact with the cell filter 72 inside the rotating disc 71. The bottom of the delivery pipe 74 and the top of the discharge pipe 8 are in contact with the mounting pipe 73 inside the other rotating disc 71. The micro motor 79 drives the second intermittent gear 78 to rotate, which drives the coaxial first intermittent gear 76 to rotate synchronously through the connecting rod 77. The second intermittent gear 78 and the first intermittent gear 76 only mesh with the driven gear 75 at a specific angle. Because the two sets of rotating discs 71 and driven gears 75 are symmetrical about the transverse central axis of the connecting rod 77 and only one set is engaged, the two sets of rotating discs 71 work alternately. The bottom of the input pipe 6 is in contact with the cell filter 72 of the upper rotating disc 71. After the sample flows in, the non-pluripotent stem cells are intercepted. The filtrate is guided through the delivery pipe 74 and the mounting pipe 73 to the discharge pipe 8 and flow into the sterile collection bottle 3.

[0023] The micro motor 79 operates periodically, and the intermittent meshing of gears causes the rotating disk 71 to periodically switch between filtration and flow guiding states. When the micro motor 79 drives the second intermittent gear 78 to rotate to a specific angle, its teeth mesh with the upper driven gear 75, pushing the upper rotating disk 71 into the filtration state. When the upper driven gear 75 rotates, the lower driven gear 75 disengages, causing the lower rotating disk 71 to enter the flow guiding state, ensuring continuous filtration, preventing the cell filter 72 from clogging, and improving efficiency.

[0024] Before the device is put into operation, the second flange 54 is fixed to the top of the input pipe 6 and is threadedly connected to the first flange 53 by bolts 52, thereby achieving a rigid connection between the output pipe 51 of the collection bag 4 and the input pipe 6. The clamp 55 wraps around the merged second flange 54 and the first flange 53, and its internal sealing ring 56 fills the gap between the second flange 54 and the first flange 53, thereby forming a sterile sealed environment to prevent external contamination from entering the filter cartridge 2. When the collection bag 4 needs to be replaced, it is only necessary to loosen the bolts 52 and remove the clamp 55 to separate the first flange 53 and the second flange 54, which meets the requirements of quick replacement and reuse.

[0025] When the device is in operation, when the teeth of the first intermittent gear 76 mesh with the driven gear 75 on the upper layer, the upper rotating disk 71 is in a filtering state. The bottom of the input pipe 6 contacts the cell filter 72, and the top of the delivery pipe 74 contacts the cell filter 72, allowing the sample to flow into the cell filter 72 for filtration. Simultaneously, the teeth of the second intermittent gear 78 are not meshed with the driven gear 75 on the right, and the lower rotating disk 71 is in a guiding state. At this time, the bottom of the delivery pipe 74 connects with the mounting pipe 73 inside the lower rotating disk 71, and the filtrate is discharged through this path. When the cell filter 72 inside the upper rotating disk 71 has been used for a long time, to prevent the cell filter 72 from becoming completely clogged, the micro motor 79 is activated. Normally, the micro motor 79 operates on a 10-minute cycle, meaning the rotating disk 71 is switched every 10 minutes. The teeth of the second intermittent gear 78 mesh with the driven gear 75 of the lower layer, driving the lower rotating disk 71 to rotate. This brings the cell filter 72 inside the lower rotating disk 71 into contact with the bottom of the delivery pipe 74, thus putting the lower rotating disk 71 into a filtering state. Due to the rotation of the second intermittent gear 78, the first intermittent gear 76 rotates, causing the driven gear 75 of the upper layer to rotate, which in turn causes the upper rotating disk 71 to rotate. At this time, the top of the delivery pipe 74 and the bottom of the input pipe 6 are in contact with the mounting pipe 73 inside the upper rotating disk 71, and the upper rotating disk 71 is in a flow guiding state. This achieves the alternating operation of the two sets of rotating disks 71. This alternating working mode ensures that the device can continuously perform filtering operations, greatly improving work efficiency, while avoiding work interruptions caused by the blockage of a single filter.

[0026] At the start of collection, the sample in collection bag 4 enters filter cartridge 2 through connecting tube 9, output tube 51, and input tube 6 under the influence of gravity. At this time, the upper rotating disk 71 is in filtration mode, the bottom of input tube 6 is in contact with cell filter 72, and the top of delivery tube 74 is in contact with cell filter 72, allowing the sample to flow into the cell filter 72 for filtration. The cell filter 72 retains non-pluripotent stem cell components in the sample, while pluripotent stem cells pass through the filter and enter delivery tube 74. Simultaneously, the lower rotating disk 71 is in a flow guiding state, and the bottom of delivery tube 74 connects with the installation tube 73 inside the lower rotating disk 71. Pluripotent stem cells flow into sterile collection bottle 3 through installation tube 73 and discharge tube 8. After all the sample in collection bag 4 has been filtered, the power switch of micro motor 79 is turned off, and the valve of discharge tube 8 is opened to discharge the remaining liquid in filter cartridge 2 and the pipes into sterile collection bottle 3. Then, sterile collection bottle 3 and collection bag 4 are disassembled sequentially for subsequent processing and testing of the collected pluripotent stem cell samples.

[0027] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0028] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A collection device for sub-pluripotent stem cells, comprising a filter cartridge: characterized in that: A sealing cap is fixedly installed at the bottom of the filter cartridge, an inlet pipe is fixedly installed at the top of the filter cartridge, an outlet pipe is fixedly installed at the bottom of the filter cartridge, a sterile collection bottle is provided outside the outlet pipe, a filter assembly is provided inside the filter cartridge, a fixing and disassembly assembly is provided at the top of the inlet pipe, a connecting pipe is provided at the top of the fixing and disassembly assembly, and a collection bag is fixedly installed at the top of the connecting pipe.

2. The collection device for sub-pluripotent stem cells according to claim 1, characterized in that: The fixed disassembly assembly includes a second flange, which is fixedly installed on the top of the input pipe. The second flange is threaded with bolts through a threaded hole inside it. The bolts are threaded with a first flange. The second flange contacts and merges with the first flange. The second flange is fixed to the first flange by bolts.

3. The collection device for sub-pluripotent stem cells according to claim 2, characterized in that: The second flange and the first flange are provided with a pipe clamp on the outside. The pipe clamp wraps around the merged second flange and the first flange. A sealing ring is fixedly installed inside the pipe clamp. The sealing ring covers the gap left after the second flange and the first flange are merged. An output pipe is fixedly installed on the top of the first flange. A connecting pipe is fixedly installed on the top of the output pipe.

4. The collection device for sub-pluripotent stem cells according to claim 3, characterized in that: The filter assembly includes a micro motor, which is fixedly installed at the bottom of the filter cartridge. A second intermittent gear is fixedly installed at one end of the output shaft of the micro motor. A connecting rod is fixedly installed at the top of the second intermittent gear. A first intermittent gear is fixedly installed at the top of the connecting rod. Two driven gears are rotatably connected inside the filter cartridge. The two driven gears are located outside the first intermittent gear and the second intermittent gear, respectively.

5. The collection device for sub-pluripotent stem cells according to claim 4, characterized in that: The first intermittent gear is meshed with the driven gear. A rotating disk is fixedly installed on the top of the driven gear. Multiple cell filters are fixedly installed on the rotating disk through through holes provided inside it. Multiple mounting tubes are fixedly installed on the rotating disk through through holes provided inside it. The rotating disk, driven gear, mounting tubes and cell filters are all symmetrically arranged in twos about the transverse central axis of the connecting rod.

6. The collection device for sub-pluripotent stem cells according to claim 5, characterized in that: Another driven gear is not meshed with the second intermittent gear. A conveying pipe is fixedly installed inside the filter cylinder. The bottom of the input pipe and the top of the conveying pipe are in contact with the cell filter inside the rotating disk. The bottom of the conveying pipe and the top of the discharge pipe are in contact with the mounting pipe inside another rotating disk.

Images