A device for fixing hollow organs in electron microscopy specimens

Abstract

This utility model relates to the field of biological tissue fixation technology, and discloses a device for fixing hollow organs in electron microscopy specimens. The device includes a main body, and further includes a control connection mechanism and a fixation adapter mechanism. The control connection mechanism includes an elastic pick-and-place component located at the upper end of the main body and a connection control component fixedly installed at its lower end. The fixation adapter mechanism includes a conductive support component movably located at the lower end of the connection control component and a fixation adapter component fixedly connected to its lower end. This device for fixing hollow organs in electron microscopy specimens employs a control connection mechanism and a fixation adapter mechanism, which can effectively and stably immerse hollow organ tissues in the fixative, improve the fixation effect, ensure the presentation of subcellular structures, and ensure that the device can be adapted to centrifuge tubes from different manufacturers, effectively meeting the needs of electron microscopy specimen fixation and improving the device's versatility and adaptability.

Description

Technical Field

[0001] This utility model relates to the field of biological tissue fixation technology, and in particular to a device for fixing hollow organs in electron microscopy specimens. Background Technology

[0002] In electron microscopy specimen preparation, especially in the immersion fixation environment for hollow organ samples such as blood vessels, intestines, and lungs, the sample must be fully immersed in the fixative to ensure effective fixation and clearly reveal subcellular structures such as mitochondria and endoplasmic reticulum. However, some problems exist in existing techniques:

[0003] First, existing fixation devices are designed for hollow organs. Because these organs are hollow and contain gas, they cannot sink to the bottom of the fixation solution like solid organs such as the liver and kidneys. This makes it impossible to fully immerse the entire hollow tissue, resulting in uneven fixation and affecting the presentation of subcellular structures. Second, electron microscope tissue blocks are small in size and are prone to leakage from existing devices. Furthermore, they are difficult to adapt to centrifuge tubes from different manufacturers with different inner diameters, making it impossible to stably and accurately fix hollow organ tissues.

[0004] Therefore, there is an urgent need to design a device for fixing hollow organs in electron microscope specimens that can solve the above technical problems. Utility Model Content

[0005] In view of the problems mentioned in the background art, the purpose of this utility model is to provide a device for fixing hollow organs in electron microscope specimens, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model mainly provides the following technical solutions:

[0007] A device for fixing hollow organs in electron microscopy specimens includes a main body and further includes:

[0008] The control connection mechanism and the fixed adapter mechanism; wherein:

[0009] The control and connection mechanism includes an elastic pick-and-place component located at the upper end of the main body and a connection and control component fixedly installed at its lower end.

[0010] The fixed adapter mechanism includes a conductive support component movably located at the lower end of the connection and control component and a fixed adapter component fixedly connected to its lower end.

[0011] Furthermore, the elastic pick-and-place assembly includes a pressing rod located at the upper end of the body, and a return spring is provided on the inner top wall of the pressing rod;

[0012] The lower end of the pressing rod is sleeved with a pressing cylinder, and the other end of the reset spring is fixedly connected to the bottom wall of the pressing cylinder.

[0013] Furthermore, the connection control assembly includes a fixing plate fixedly connected to the bottom wall of the pressing cylinder, and a spiral sleeve rod is provided in the center of the bottom wall of the fixing plate. The spiral sleeve rod has an internal thread that penetrates the center of the bottom end of the spiral inner cylinder.

[0014] Furthermore, the conductive support assembly includes a spiral connecting rod that is threadedly connected to the spiral inner cylinder, and the lower end of the spiral connecting rod branches into four support rods on an even scale around its outer periphery;

[0015] The outer wall of the spiral connecting rod is provided with external threads.

[0016] Furthermore, the fixed adapter component includes a fixed ring that is fixedly connected to the other end of the support rod. A mesh groove is formed in the lateral recess of the inner ring of the fixed ring, and a nylon mesh is fixedly installed in the mesh groove. An adapter groove is formed in the lateral recess of the outer ring of the fixed ring, and a rubber ring is movably installed in the adapter groove.

[0017] Furthermore, the bodies are all made of materials that are resistant to chemical corrosion and have good biocompatibility.

[0018] Compared with the prior art, the beneficial effects of this utility model are mainly reflected in:

[0019] Compared to existing fixation devices, which struggle to fully immerse hollow organ tissues in fixative, suffer from poor fixation results, are unsuitable for small tissues under electron microscopes, are prone to leakage, and are difficult to adapt to different centrifuge tubes, this invention provides a fixation device for hollow organs in electron microscope specimens. This device employs a structure combining an adjustable connection mechanism and a fixation adapter mechanism. An elastic loading and unloading component facilitates device placement and removal, the connection and adjustment component allows for length adjustment, the transmission and support component transmits pressure, and the fixation adapter component utilizes a nylon mesh to hold the tissue in place and a rubber ring to fit the inner diameter of the centrifuge tube. This effectively ensures stable immersion of hollow organ tissues in the fixative, improves fixation results, guarantees the preservation of subcellular structures, and ensures compatibility with centrifuge tubes from different manufacturers. This effectively meets the needs of electron microscope specimen fixation, enhancing the device's versatility and adaptability.

[0020] The above description is only an overview of the technical solution of this utility model. In order to clearly understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1This is a perspective view of a device for fixing hollow organs in electron microscope specimens according to the present invention;

[0023] Figure 2 This is a cross-sectional view of a device for fixing hollow organs in electron microscope specimens according to the present invention;

[0024] Figure 3 This is a structural diagram of a device for fixing hollow organs in electron microscope specimens according to the present invention;

[0025] Figure 4 This is an exploded view of a device for fixing hollow organs in electron microscope specimens according to the present invention.

[0026] Figure 5 This is a diagram illustrating the use of a device for fixing hollow organs in electron microscope specimens according to the present invention.

[0027] Explanation of reference numerals in the attached figures:

[0028] 1. Main body; 2. Adjustment and connection mechanism; 3. Fixing and adapter mechanism;

[0029] 21. Flexible pick-and-place component; 22. Connection and control component;

[0030] 31. Conductive support component; 32. Fixing adapter component;

[0031] 211. Pressing rod; 212. Return spring; 213. Pressing cylinder;

[0032] 221. Fixing plate; 222. Spiral sleeve; 223. Spiral inner cylinder; 224. Internal thread;

[0033] 311. Helical connecting rod; 312. Support rod; 313. External thread;

[0034] 321. Fixing ring; 322. Mesh groove; 323. Nylon mesh; 324. Adaptor groove; 325. Rubber ring; Detailed Implementation

[0035] The present application will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the relevant utility model and not intended to limit the scope of the utility model. Furthermore, it should be noted that, for ease of description, only the parts relevant to the utility model are shown in the accompanying drawings.

[0036] like Figure 1-5 As shown, this utility model provides a device for fixing hollow organs in electron microscopy specimens, comprising a body 1, and further comprising:

[0037] Adjustment connection mechanism 2 and fixed adapter mechanism 3; wherein:

[0038] The control and connection mechanism 2 includes an elastic pick-and-place component 21 located at the upper end of the main body 1 and a connection and control component 22 fixedly installed at its lower end. The elastic pick-and-place component 21 enables convenient pick-and-place of the device in the centrifuge tube, and the connection and control component 22 enables connection with the fixed adapter mechanism 3 below and adjustment of parameters such as length.

[0039] The fixed adapter mechanism 3 includes a conductive support component 31 movably located at the lower end of the connecting control component 22 and a fixed adapter component 32 fixedly connected to its lower end. The conductive support component 31 bears the transmission and distribution of force, ensuring that the fixing force acts evenly downward. The fixed adapter component 32 directly contacts the hollow organ tissue to achieve stable fixation of the tissue and adaptation to the centrifuge tube.

[0040] like Figure 1 , 2 As shown in Figure 5, in this embodiment, the elastic pick-and-place assembly 21 includes a pressing rod 211 disposed on the upper end of the body 1. The pressing rod 211 is the operation execution component of the elastic pick-and-place assembly 21, which is used by the operator to apply external force. A return spring 212 is provided on the inner top wall of the pressing rod 211. The return spring 212 provides elastic restoring force for the pressing rod 211. When an external force is applied to press down the pressing rod 211, the pressing rod 211 can descend. When the force is removed, the return spring 212 can push the pressing rod 211 back to its initial position.

[0041] The lower end of the pressing rod 211 is sleeved with a pressing cylinder 213. The pressing cylinder 213 guides and limits the pressing rod 211, restricting its movement trajectory so that it can only reciprocate longitudinally. The other end of the return spring 212 is fixedly connected to the inner bottom wall of the pressing cylinder 213. When the pressing rod 211 is squeezed by the centrifuge tube cover, the return spring 212 contracts, and the tube cover can be closed tightly. After the tube cover is opened, the return spring 212 extends to push the pressing rod 211 back to its original position, making it easy for the user to squeeze and remove the device.

[0042] like Figure 1 , 2 As shown in Figure 3, in this embodiment, the connection control component 22 includes a fixing plate 221 fixedly connected to the bottom wall of the pressing cylinder 213. The fixing plate 221 is fixed to the pressing cylinder 213 on one hand, bearing the structure and force of the elastic pick-and-place component 21 on the other hand, and providing an installation base for the spiral sleeve rod 222. The spiral sleeve rod 222 is provided in the center of the bottom wall of the fixing plate 221. The spiral sleeve rod 222 realizes the length adjustment and connection function. It can cooperate with the lower conduction support component 31. The spiral inner cylinder 223 is opened through the center of the bottom end of the spiral sleeve rod 222 and is provided with an internal thread 224, which is used to connect with the spiral connecting rod 311 in the conduction support component 31. Through the threaded cooperation, the length adjustment between the connection control component 22 and the conduction support component 31 can be realized, thereby adapting to the fixing needs of centrifuge tubes of different depths and tissues of different sizes.

[0043] like Figure 2 , 3 As shown, in this embodiment, the conductive support assembly 31 includes a spiral connecting rod 311 that is threadedly connected to the spiral inner cylinder 223. The spiral connecting rod 311 connects to the connecting adjustment assembly 22 through its cooperation with the spiral inner cylinder 223. At the same time, the length direction can be adjusted by rotating the thread. The lower end of the spiral connecting rod 311 branches into four support rods 312 on the outer periphery. The four support rods 312 are evenly distributed, which can evenly distribute the force transmitted from the spiral connecting rod 311 to the fixed adapter assembly 32, ensuring the uniform transmission of the fixing force.

[0044] The outer wall of the spiral connecting rod 311 is provided with an external thread 313, which matches the internal thread 224 of the inner cylinder of the spiral sleeve rod 222, so as to realize the detachable connection and length adjustment function between the two.

[0045] like Figure 1 , 3 As shown in Figure 4, in this embodiment, the fixing adapter component 32 includes a fixing ring 321 fixedly connected to the other end of the support rod 312. The fixing ring 321 is used to install components such as the nylon mesh 323 and the rubber ring 325. Simultaneously, through its connection with the support rod 312, it receives and transmits the force transmitted by the support component 31. A mesh groove 322 is recessed on the inner circumference of the fixing ring 321. This recessed structure can limit the position of the nylon mesh 323, ensuring that the nylon mesh 323 is stable after installation and will not easily shift. The nylon mesh 323 is fixedly installed within the mesh groove 322. The nylon mesh 323, made of 100 mesh, is flexible and can effectively hold a 1 cubic millimeter of hollow organ tissue for electron microscopy, ensuring full contact between the tissue and the fixative. It also avoids damaging the tissue due to its high hardness. The outer circumference of the fixation ring 321 has a recessed fitting groove 324, which provides installation space and limits for the rubber ring 325, ensuring stable installation. The rubber ring 325 is movably installed within the fitting groove 324. The elastic rubber ring 325 can adapt to 1.5ml / 2ml centrifuge tubes from different manufacturers with slight differences in inner diameter, keeping the fixation ring 321 stable within the centrifuge tube and preventing wobbling due to differences in tube inner diameter. This ensures the stability of the entire fixation device for tissue fixation.

[0046] In this embodiment, the main body 1 is made of a material that is resistant to chemical corrosion and has good biocompatibility. The chemical corrosion resistant material can ensure the stability of the device structure and prevent damage due to chemical corrosion; the good biocompatibility can avoid adverse reactions between the material and biological tissue specimens, ensure the original state of the specimens, and ensure the accuracy of electron microscopy observation results.

[0047] The working principle and usage process of this technical solution are as follows: During use, first prepare a general 1.5ml / 2ml centrifuge tube containing fixative, and put a 1 cubic millimeter hollow electron microscope specimen of hollow organ tissue into the fixative in the centrifuge tube;

[0048] Next, the fixing device is picked up by the pressing rod 211 of the elastic pick-and-place component 21, aligned with the centrifuge tube opening, and placed into the centrifuge tube. After the device is fully placed, the rubber ring 325 of the fixing adapter component 32 contacts the inner wall of the centrifuge tube. The elasticity of the rubber ring 325 adapts to the inner diameter of the centrifuge tube, stabilizing the fixing ring 321 and the entire fixing adapter component 32 inside the centrifuge tube. Utilizing the threaded connection between the spiral sleeve 222 of the connecting adjustment component 22 and the spiral connecting rod 311 of the conduction support component 31, the spiral sleeve 222 is rotated to adjust the relative length between the conduction support component 31 and the connecting adjustment component 22, so that the bottom wall of the nylon mesh 323 of the fixing adapter component 32 contacts and gently presses down on the tissue. Finally, when closing the centrifuge tube cap, the pressing rod 211 is squeezed by the cap, and the return spring 212 contracts, ensuring that the cap can be closed smoothly and tightly.

[0049] The tissue is held in place by the nylon mesh 323 and pressed down below the surface of the fixative liquid to achieve full fixation. When it is necessary to remove the device and tissue, the centrifuge tube cap is opened, and the pressing rod 211 extends and resets under the action of the return spring 212, making it easy for the operator to squeeze the pressing rod 211 to remove the device and tissue, thus completing the fixation procedure for a hollow organ of an electron microscope specimen.

[0050] The present invention has been further described above with reference to the embodiments, but the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. A device for the fixation of organs in the cavities of specimens for electron microscopy, comprising a body (1), characterized in that: Also includes: The control connection mechanism (2) and the fixed adapter mechanism (3) are used; wherein: The control connection mechanism (2) includes an elastic pick-and-place component (21) located at the upper end of the body (1) and a connection control component (22) fixedly installed at its lower end; The fixed adapter mechanism (3) includes a conductive support component (31) movably disposed at the lower end of the connection control component (22) and a fixed adapter component (32) fixedly connected to its lower end.

2. The device for fixing the specimen cavity according to claim 1, wherein: The elastic pick-and-place assembly (21) includes a pressing rod (211) located at the upper end of the body (1), and a return spring (212) is provided on the inner top wall of the pressing rod (211); The lower end of the pressing rod (211) is sleeved around the pressing cylinder (213), and the other end of the reset spring (212) is fixedly connected to the inner bottom wall of the pressing cylinder (213).

3. The specimen cavity organ holder for electron microscopy of claim 1, wherein: The connection control assembly (22) includes a fixing plate (221) fixedly connected to the bottom wall of the pressing cylinder (213). A spiral sleeve rod (222) is provided in the center of the bottom wall of the fixing plate (221). A spiral inner cylinder (223) is opened through the center of the bottom end of the spiral sleeve rod (222) and is provided with an internal thread (224).

4. The specimen cavity organ holder for electron microscopy of claim 1, wherein: The conductive support assembly (31) includes a spiral connecting rod (311) that is threadedly connected to the spiral inner cylinder (223), and the lower end of the spiral connecting rod (311) branches into four support rods (312) on an even scale on the outer periphery; The outer wall of the spiral connecting rod (311) is provided with an external thread (313).

5. The specimen cavity organ holder for electron microscopy of claim 1, wherein: The fixed adapter component (32) includes a fixed ring (321) that is fixedly connected to the other end of the support rod (312). A mesh groove (322) is recessed on the inner circumference of the fixed ring (321). A nylon mesh (323) is fixedly installed in the mesh groove (322). An adapter groove (324) is recessed on the outer circumference of the fixed ring (321). A rubber ring (325) is movably installed in the adapter groove (324).

6. The specimen cavity organ holder for electron microscopy of claim 1, wherein: The main body (1) is made of a material that is resistant to chemical corrosion and has good biocompatibility.

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