Quantum dot fluorescent detection card for quantitatively detecting MxA

Abstract

The utility model relates to protein detection technical field discloses a kind of quantum dot fluorescence detection card of quantitative detection MxA, including detection card, the detection card includes box, upper cover, the recess is established in the box top, the recess inner bottom is equipped with a plurality of placing groove, test strip is equipped in the placing groove, the recess is symmetrically equipped with the limiting strip connected with upper cover, the box and upper cover are connected by fixed mechanism;The utility model is cooperated with limiting strip by placing groove, and the test strip is limited and both ends are pressed solid, effectively prevent test strip from being displaced due to infiltration after droping sample, avoid interfering normal reaction of test strip and sample, guarantee the accuracy of detection result;Meanwhile, with the help of fixed mechanism, the box and upper cover can be conveniently fixed or split, further ensure that the limiting strip is stably pressed solid to the test strip, improve detection reliability and operational convenience.

Description

Technical Field

[0001] This utility model relates to the field of protein detection technology, and in particular to a quantum dot fluorescent detection card for quantitative detection of MxA. Background Technology

[0002] Antiviral protein A (MxA) is a 78KD protein induced by type 1 interferon-IFN (α / β) and distributed in the cytoplasm. It has broad-spectrum antiviral activity and is one of the important antiviral proteins. It can inhibit various RNA viruses and some DNA viruses, and its expression level can accurately reflect the IFN-induced state of the body. In vitro studies have shown that HIV infection of human monocytes can produce MxA protein as early as 6 hours after infection, and reach its peak expression at 14 days. Moreover, this expression process can be independent of IFN-α. Extremely low viral load (1 pfu / ml) can induce cells to express detectable MxA protein, making it an important indicator for the early and acute diagnosis of viral infection.

[0003] Although MxA is extremely valuable in the field of virus detection, existing detection technologies still have shortcomings. Currently, most test cards used for MxA detection adopt a traditional structure, with the test strip placed in the slot of the test card box. When a quantitative sample is added through the sample application hole on the top cover, the sample wetting can easily cause the test strip to shift, thereby interfering with the normal reaction process between the test strip and the sample, ultimately causing deviations in the test results and seriously affecting the accuracy of the detection. Utility Model Content

[0004] In view of the problem that the existing test strips for detecting MxA are prone to displacement after being soaked in the sample, which interferes with the normal reaction between the strips and the sample, resulting in deviations and insufficient accuracy in the test results, this utility model is proposed.

[0005] To solve the above technical problems, this utility model provides the following technical solution: a quantum dot fluorescence detection card for quantitative detection of MxA, comprising a detection card, the detection card including a box body and a top cover, the top of the box body having a groove, the bottom of the groove having a plurality of placement slots having a test strip placed in the placement slots, and the groove having symmetrically arranged limiting strips connected to the top cover, the box body and the top cover being connected by a fixing mechanism.

[0006] As a preferred embodiment, a positioning post is fixedly installed at each of the four corners of the bottom of the top cover, and a positioning groove adapted to the positioning post is opened on the top of the box body.

[0007] As a preferred embodiment, the fixing mechanism includes a rotating rod, and the two ends of the box are symmetrically provided with mounting grooves that communicate with the positioning grooves. The rotating rod is rotatably installed in the mounting grooves. The outer walls of the two ends of the rotating rod are symmetrically provided with misalignment grooves, and the misalignment grooves are adapted to the positioning posts. The outer wall of the positioning posts is provided with limit grooves corresponding to the misalignment grooves.

[0008] As a preferred embodiment, the limiting groove is arc-shaped, and the limiting groove is adapted to the corresponding misalignment groove portion of the rotating rod.

[0009] As a preferred embodiment, a drive plate is fixedly connected to the outer wall at the middle position of the rotating rod, and a receiving groove is provided on both sides of the mounting groove at the end of the box body, and the receiving groove is connected to the mounting groove, with the drive plate located in the receiving groove.

[0010] As a preferred embodiment, the inner wall surface of the drive plate at the outermost end is arc-shaped.

[0011] As a preferred embodiment, the top of the cover has several sample inlets, which are funnel-shaped and directly opposite the test strip.

[0012] As a preferred embodiment, the test strip includes a coating film layer and an MxA detection layer, with the MxA detection layer located on top of the coating film layer.

[0013] Compared with the prior art, the present invention has at least the following beneficial effects:

[0014] 1. This utility model uses a placement groove to limit the position of the test strip. In conjunction with the limiting strip connected to the top cover, the two ends of the test strip can be pressed and fixed. The two work together to restrict and fix the test strip, thereby avoiding the problem that the test strip may shift after the sample is added, which would interfere with the normal reaction process between the test strip and the sample, and ultimately cause deviation in the test results, seriously affecting the accuracy of the test.

[0015] 2. This utility model, through a fixing mechanism, can conveniently and quickly restrict and fix the positioning post, thereby facilitating the connection, fixing, or disassembly between the box body and the top cover, ensuring that the limiting strip has a good pressing and fixing effect on the test strip. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 This is a structural schematic diagram of the box body and one end of the top cover of this utility model;

[0018] Figure 3 This is a top view of the box body of this utility model;

[0019] Figure 4 This is a schematic diagram of the structure between the rotating rod and the drive plate of this utility model;

[0020] Figure 5 This is a schematic diagram of the positioning post and limiting groove structure of this utility model;

[0021] Figure 6 This is a cross-sectional structural diagram of the test strip of this utility model.

[0022] Explanation of reference numerals in the attached figures:

[0023] 1. Test card; 2. Box body; 3. Top cover; 4. Test strip; 5. Limiting strip; 6. Groove; 7. Placement slot; 8. Positioning post; 9. Positioning slot; 10. Rotating rod; 11. Mounting slot; 12. Misalignment slot; 13. Limiting slot; 14. Drive plate; 15. Receiving slot; 16. Coating film layer; 17. MxA detection layer; 18. Sample dispensing port. Detailed Implementation

[0024] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0025] Reference Figures 1-6 As shown, a quantum dot fluorescence detection card for quantitative detection of MxA is provided, including a detection card 1. The detection card 1 includes a box body 2 and a top cover 3. A groove 6 is formed on the top of the box body 2, and several placement slots 7 are formed at the bottom of the groove 6. Test strips 4 are placed in the placement slots 7. Limiting strips 5 connected to the top cover 3 are symmetrically arranged in the grooves 6. The box body 2 and the top cover 3 are connected by a fixing mechanism. The placement slots 7 limit the test strips 4, and the limiting strips 5 press the two ends together to effectively prevent the test strips 4 from shifting and ensure the accuracy of detection.

[0026] In this example, a positioning post 8 is fixedly installed at each of the four bottom corners of the top cover 3, and a positioning groove 9 adapted to the positioning post 8 is opened on the top of the box body 2. The positioning post 8 and the positioning groove 9 cooperate to provide a positioning basis for the precise installation of the box body 2 and the top cover 3, and enhance the structural stability.

[0027] In this example, the fixing mechanism includes a rotating rod 10. The two ends of the box body 2 are symmetrically provided with mounting grooves 11 that communicate with the positioning grooves 9. The rotating rod 10 is rotatably installed in the mounting grooves 11. The outer walls of the two ends of the rotating rod 10 are symmetrically provided with misaligned grooves 12, and the misaligned grooves 12 are adapted to the positioning posts 8. The outer wall of the positioning posts 8 is provided with limiting grooves 13 corresponding to the misaligned grooves 12. The fixing mechanism, through the cooperation of the rotating rod 10, the misaligned grooves 12 and the limiting grooves 13, realizes the quick connection and fixation of the box body 2 and the top cover 3 and ensures that the limiting strip 5 stably presses the test strip 4.

[0028] In this example, the limiting groove 13 is arc-shaped, and the limiting groove 13 is adapted to the part of the misalignment groove 12 corresponding to the rotating rod 10. The arc-shaped limiting groove 13 is designed to fit tightly with the misalignment groove 12, so that the part of the rotating rod 10 corresponding to the misalignment groove 12 is inserted into the limiting groove 13, thereby providing a stable locking effect on the positioning post 8 and preventing the box 2 from accidentally separating from the top cover 3.

[0029] In this example, a drive plate 14 is fixedly connected to the outer wall of the middle position of the rotating rod 10. The end of the box body 2 is provided with receiving grooves 15 on both sides of the mounting groove 11, and the receiving grooves 15 are connected to the mounting groove 11. The drive plate 14 is located in the receiving groove 15. The setting of the drive plate 14 and the receiving groove 15 makes it convenient for the operator to apply force to rotate the rotating rod 10, improves the convenience of fixing and disassembling operations, and can accommodate the drive plate 14.

[0030] In this example, the inner wall surface of the drive board 14 at the outer end is rounded; the rounded inner wall surface design is ergonomic, further optimizing the operating feel and reducing operating resistance.

[0031] In this example, the top of the cover 3 has several sample inlets 18, and the sample inlets 18 are funnel-shaped, with the sample inlets 18 facing the test strip 4. The funnel-shaped sample inlets 18 design guides the sample to flow precisely to the test strip 4, reduces sample splashing, and improves detection efficiency and accuracy.

[0032] In this example, the test strip 4 includes a coating membrane layer 16 and an MxA detection layer 17, with the MxA detection layer 17 located on top of the coating membrane layer 16; the MxA detection layer 17 interacts with the coating membrane layer 16 to react with the sample and detect it.

[0033] During use, the test strip 4 is fixed by placing the test strip 4 into the placement groove 7 in the groove 6 of the box body 2, and then covering it with the top cover 3. The limiting strip 5 is connected to the top cover 3 and presses the two ends of the test strip 4. The placement groove 7 and the limiting strip 5 work together to prevent the test strip 4 from shifting due to wetting after the quantitative sample is added, thus ensuring that it reacts normally with the sample.

[0034] The connection principle between the box body 2 and the top cover 3 is as follows: The positioning post 8 at the bottom of the top cover 3 is inserted into the positioning groove 9 at the top of the box body 2. The flip drive plate 14 moves downward, thereby driving the rotating rod 10 to rotate. This causes the misalignment grooves 12 at both ends of the rotating rod 10 to rotate to the side away from the positioning post 8. At the same time, the part of the rotating rod 10 corresponding to the misalignment groove 12 moves into the limiting groove 13 opened on the positioning post 8, thereby restricting and fixing the positioning post 8. This enables the box body 2 and the top cover 3 to be quickly connected, ensuring that the limiting strip 5 stably presses the test strip 4. To release the restriction, simply flip the drive plate 14 upward to drive the rotating rod 10 to rotate, causing the part of the rotating rod 10 located in the limiting groove 13 to move out and make the misalignment groove 12 correspond to the positioning post 8, thereby releasing the restriction on the positioning post 8.

[0035] Sample addition and detection principle: A quantitative sample is added through the funnel-shaped sample inlet 18 at the top of the cover 3. Since the sample inlet 18 is directly opposite the test strip 4, the sample flows precisely to the test strip 4. Then, it is detected by a fluorescence immunoassay analyzer. The MxA content in the sample can be reflected by detecting its fluorescence signal value.

[0036] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A quantum dot fluorescence test card for quantitative detection of MxA, comprising a test card (1), characterized in that: The detection card (1) contains a box body (2), an upper cover (3), the top of the box body (2) is provided with a groove (6), a plurality of placing grooves (7) are arranged in the bottom of the groove (6), test strips (4) are arranged in the placing grooves (7), the groove (6) is symmetrically provided with a limiting strip (5) connected with the upper cover (3), and the box body (2) and the upper cover (3) are connected through a fixing mechanism.

2. The quantum dot fluorescent test card for quantitatively detecting MxA according to claim 1, characterized in that: The upper cover (3) is fixedly installed with a positioning column (8) at the bottom of each corner, and the top of the box body (2) is provided with a positioning groove (9) corresponding to the positioning column (8).

3. The quantum dot fluorescent test card for quantitatively detecting MxA according to claim 2, characterized in that: The fixing mechanism comprises a rotating rod (10), the two ends of the box body (2) are symmetrically provided with an installation groove (11) in communication with the positioning groove (9), the rotating rod (10) is rotatably installed in the installation groove (11), the two ends of the rotating rod (10) are symmetrically provided with a misaligned groove (12) on the outer wall, and the misaligned groove (12) is matched with the positioning column (8), and the outer wall of the positioning column (8) is provided with a limiting groove (13) corresponding to the misaligned groove (12).

4. The quantum dot fluorescent test card for quantitatively detecting MxA according to claim 3, characterized in that: The limiting groove (13) is arc-shaped, and the limiting groove (13) is matched with the corresponding misaligned groove (12) of the rotating rod (10).

5. The quantum dot fluorescent test card for quantitatively detecting MxA according to claim 4, characterized in that: The rotating rod (10) is fixedly connected with a driving plate (14) on the outer wall at the middle position, the box body (2) is provided with an accommodating groove (15) on the two sides of the installation groove (11) at the end, and the accommodating groove (15) is in communication with the installation groove (11), and the driving plate (14) is located in the accommodating groove (15).

6. The quantum dot fluorescent test card for quantitatively detecting MxA according to claim 5, characterized in that: The inner wall surface of the one end of the driving plate (14) is arc-shaped.

7. The quantum dot fluorescent test card for quantitatively detecting MxA according to claim 2, characterized in that: The upper cover (3) is provided with a plurality of sample adding openings (18) at the top, and the sample adding openings (18) are funnel-shaped, the sample adding openings (18) are opposite to the test strips (4).

8. The quantum dot fluorescent test card for quantitatively detecting MxA according to claim 2, characterized in that: The test strip (4) comprises a coating film layer (16) and an MxA detection layer (17), and the MxA detection layer (17) is located at the top of the coating film layer (16).

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