A pre-treatment device for ethanol components in a sample

Abstract

A kind of pre-treatment device of ethanol component in sample, belong to ethanol processing equipment technical field, including heating base, sample heating cup is placed on heating base, sample heating cup includes heating cup bottom, heating cup inner wall and heating cup outer wall, the height of heating cup inner wall is lower than the height of heating cup outer wall, top cover that can seal the cavity inside heating cup outer wall is equipped on heating cup outer wall;Heating cup inner wall and heating cup bottom are enclosed into inner cup capable of containing sample solution;Heating cup inner wall, heating cup outer wall and heating cup bottom are enclosed into first containing cavity capable of containing ethanol absorption liquid.Sample solution is added to inner cup, ethanol absorption liquid is added to first containing cavity, top cover is covered to make sample heating cup form sealed state, and ethanol volatilized is absorbed by ethanol absorption liquid;Through the absorption of ethanol absorption liquid to ethanol in sealed space, not only improve the ethanol volatilization efficiency, the absorption effect of ethanol, also avoid the pollution of sample solution by external material.

Description

Technical Field

[0001] This utility model belongs to the technical field of ethanol processing equipment, specifically a pretreatment device for ethanol components in a sample. Background Technology

[0002] Bacterial endotoxins are unique structures on the cell walls of Gram-negative bacteria. Endotoxins are exogenous pyrogens that activate neutrophils and other cells, causing them to release endogenous pyrogens, which then act on the body's thermoregulatory center to induce fever. The main chemical component of bacterial endotoxins is lipopolysaccharide.

[0003] Bacterial endotoxins, as a biosafety indicator, are crucial for overall pharmaceutical safety. Therefore, controlling bacterial endotoxin levels in raw materials, intermediates, and finished products is essential during drug production. Since bacterial endotoxins react with Limulus amebocyte lysate (LAL) reagent, the LAL-based bacterial endotoxin gel assay is an important method for monitoring bacterial endotoxin levels.

[0004] However, during the pharmaceutical manufacturing process, many raw materials, intermediate samples, and even finished products contain ethanol solutions as protective agents or solvents. Ethanol solutions can disrupt the coagulation reaction between bacterial endotoxins and horseshoe crab reagent. Therefore, when conducting experiments on samples containing ethanol solutions, it is necessary to pretreat the samples to eliminate interference.

[0005] Common methods for eliminating interference include dilution and heating / evaporation. However, dilution methods often face limitations in practical experiments due to the maximum dilution factor and the requirement for high sensitivity to horseshoe crab reagent. While heating can remove ethanol from samples, its removal efficiency is poor, and it can easily damage the sample and introduce new endotoxin contamination during the heating process. Utility Model Content

[0006] To address the problem that heating methods are ineffective in removing ethanol from samples and may introduce new sources of contamination, this invention provides a pretreatment device for ethanol in samples.

[0007] This utility model is achieved through the following technical solution:

[0008] A pretreatment device for ethanol components in a sample includes a heating base on which a sample heating cup is placed. The sample heating cup includes a bottom, an inner wall, and an outer wall. The inner wall and the outer wall are both fused to the bottom of the heating cup, and the cross-sections of the inner wall and the outer wall are arranged in a concentric ring from the inside to the outside.

[0009] The height of the inner wall of the heating cup is lower than the height of the outer wall of the heating cup, and the outer wall of the heating cup is covered with a top cover that can seal the cavity inside the outer wall of the heating cup.

[0010] The inner wall and bottom of the heating cup form an inner cup capable of containing the sample solution; the inner wall, outer wall, and bottom of the heating cup form a first receiving cavity capable of containing the ethanol absorption liquid.

[0011] Add the sample solution containing ethanol to the inner cup, add the ethanol absorption liquid to the first receiving cavity, and cover the top to seal the sample heating cup. The ethanol that evaporates after heating is absorbed by the ethanol absorption liquid. By absorbing ethanol in the sealed space, not only is the ethanol evaporation efficiency and the absorption effect of ethanol improved, but the sample solution is also prevented from being contaminated by external substances.

[0012] A further improvement of this invention includes a removable annular cup that can be placed within the first receiving cavity. The removable annular cup comprises an annular bottom surface, an annular outer wall, and an annular inner wall, both of which are fused to the annular bottom surface. The annular outer wall, the annular bottom surface, and the annular inner wall form a second receiving cavity capable of containing the ethanol absorption liquid. By placing the removable annular cup into the first receiving cavity to hold the ethanol absorption liquid, the cup can be directly replaced after the ethanol absorption liquid reaches its maximum absorption limit. This improves the replacement efficiency of the ethanol absorption liquid and avoids direct contact with the sample solution in the inner cup, helping to reduce contamination of the sample solution.

[0013] A further improvement of this invention is that the height of the removable annular cup is less than the height of the outer wall of the heating cup. This avoids the top of the removable annular cup coming into contact with the top cover, which could lead to a poor seal and improves the reliability of the experimental data.

[0014] A further improvement of this invention is that the height of the annular inner wall is greater than the height of the inner wall of the heating cup. Using the portion extending above the inner wall of the heating cup for clamping operations helps to improve the ease of replacing the removable annular cup.

[0015] A further improvement of this invention is that an operating gap is provided between the aforementioned annular inner wall and the inner wall of the heating cup. This operating gap provides more operating space for the clamping tool and prevents the clamping tool from extending into the inner cup.

[0016] A further improvement of this invention is that at least one inner wall of the second receiving cavity is provided with a pure white coating. Since the ethanol absorbent changes color after absorbing ethanol, the white coating can be used as a comparative reference for color observation; as the absorbed ethanol content increases, the color depth gradually increases, which can be used as a reference to help determine whether the ethanol absorbent needs to be replaced.

[0017] A further improvement of this invention is that the sealing surface at the top of the outer wall of the heating cup, which comes into contact with the top cover, is a frosted surface. This helps to increase the seal between the top cover and the outer wall of the heating cup, reduce gas leakage, and prevent bacterial endotoxins in the outside air from contaminating the sample solution.

[0018] A further improvement of this invention is that the top surface of the heating base is also covered with an asbestos layer. The asbestos layer has the functions of heat insulation and uniform heat conduction, preventing the sample heating cup from cracking due to uneven heating.

[0019] A further improvement of this invention is that the side of the heating base is also provided with a temperature control panel for controlling the heating temperature. The optimal heating temperature for the sample solution is achieved through the temperature control panel.

[0020] As can be seen from the above technical solution, the beneficial effects of this utility model are: adding a sample solution containing ethanol into the inner cup, adding ethanol absorption liquid into the first receiving cavity, and covering the top cover to make the sample heating cup form a sealed state, the ethanol that evaporates after heating is absorbed by the ethanol absorption liquid; by absorbing ethanol by the ethanol absorption liquid in the sealed space, not only is the ethanol evaporation efficiency and the absorption effect of ethanol improved, but the sample solution is also prevented from being contaminated by external substances. Attached Figure Description

[0021] To more clearly illustrate the technical solution of this utility model, the drawings used in the description will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a structural schematic diagram of a specific embodiment of the present utility model.

[0023] Figure 2 This is an exploded view of the removable ring cup and sample heating cup according to a specific embodiment of the present invention.

[0024] In the attached diagram: 1. Heating base; 2. Temperature control panel; 3. Sample heating cup; 4. Top cover; 5. Removable annular cup; 6. Outer wall of heating cup; 7. Bottom of heating cup; 8. Inner wall of heating cup; 9. Annular bottom surface; 10. Annular outer wall; 11. Annular inner wall; 12. First receiving cavity; 13. Sealing surface; 14. Inner cup; 15. Asbestos layer; 16. Second receiving cavity. Detailed Implementation

[0025] To make the objectives, features, and advantages of this utility model more apparent and understandable, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings of the specific embodiments. Obviously, the embodiments described below are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this patent, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this patent.

[0026] like Figures 1-2 As shown, this utility model discloses a pretreatment device for ethanol components in a sample, including a heating base 1, on which a sample heating cup 3 is placed. The sample heating cup 3 includes a heating cup bottom 7, a heating cup inner wall 8, and a heating cup outer wall 6. The heating cup inner wall 8 and the heating cup outer wall 6 are both fused to the heating cup bottom 7, and the cross-sections of the heating cup inner wall 8 and the heating cup outer wall 6 are arranged in a concentric ring from the inside to the outside.

[0027] The height of the inner wall 8 of the heating cup is lower than the height of the outer wall 6 of the heating cup, and the outer wall 6 of the heating cup is covered with a top cover 4 that can seal the internal cavity of the outer wall 6 of the heating cup.

[0028] The inner wall 8 and the bottom 7 of the heating cup form an inner cup 14 that can accommodate the sample solution; the inner wall 8, the outer wall 6, and the bottom 7 of the heating cup form a first accommodating cavity 12 that can accommodate the ethanol absorption liquid.

[0029] Add a sample solution containing ethanol to the inner cup 14, add ethanol absorbent to the first receiving cavity 12, and cover the top cover 4 to seal the sample heating cup 3. The ethanol that evaporates after heating is absorbed by the ethanol absorbent. By absorbing ethanol in the sealed space, not only is the ethanol evaporation efficiency and the absorption effect of ethanol improved, but the sample solution is also prevented from being contaminated by external substances.

[0030] The ethanol absorption solution is a potassium dichromate-sulfuric acid solution. Specifically, 3.7 g of potassium dichromate is weighed, dissolved in 150 ml of water, and then 280 ml of sulfuric acid is slowly added. After cooling, water is added to 500 ml, and the solution is shaken well.

[0031] It also includes a removable annular cup 5 that can be placed within the first receiving cavity 12; the removable annular cup 5 includes an annular bottom surface 9, an annular outer wall 10, and an annular inner wall 11, the annular outer wall 10 and the annular inner wall 11 being fused to the annular bottom surface 9; the annular outer wall 10, the annular bottom surface 9, and the annular inner wall 11 form a second receiving cavity 16 capable of containing the ethanol absorption liquid. By placing the removable annular cup 5 into the first receiving cavity 12 to contain the ethanol absorption liquid, the removable annular cup 5 can be directly replaced after the ethanol absorption liquid reaches its upper limit, improving the replacement efficiency of the ethanol absorption liquid, while avoiding direct contact with the sample solution in the inner cup 14, which helps to reduce contamination of the sample solution.

[0032] Ethanol absorbent liquid is added into the removable ring cup 5, and the liquid level of the ethanol absorbent liquid is at least 0.5cm-1.5cm above the mouth of the removable ring cup 5.

[0033] The height of the removable annular cup 5 is less than the height of the outer wall 6 of the heating cup. This avoids the top of the removable annular cup 5 from contacting the top cover 4, which could lead to a poor seal and improve the reliability of the experimental data. The distance between the highest point of the removable annular cup 5 and the mouth of the outer wall 6 of the heating cup is at least 0.5 cm.

[0034] The height of the annular inner wall 11 is greater than the height of the inner wall 8 of the heating cup. Using the portion extending above the inner wall 8 for clamping operations helps improve the ease of replacing the removable annular cup 5.

[0035] The lowest point of the removable ring cup 5 is at least 1 cm away from the mouth of the inner wall 8 of the heating cup.

[0036] An operating gap is provided between the annular inner wall 11 and the inner wall 8 of the heating cup. This operating gap provides more operating space for the clamping tool and prevents the clamping tool from extending into the inner cup 14.

[0037] At least one inner wall of the second receiving cavity 16 is provided with a pure white coating. Since the ethanol absorbent changes color after absorbing ethanol, the white coating can be used as a comparative reference for color observation; as the absorbed ethanol content increases, the color depth gradually increases, which can be used as a reference to help determine whether the ethanol absorbent needs to be replaced.

[0038] The sealing surface 13 at the top of the outer wall 6 of the heating cup, which can contact the top cover 4, is frosted. This helps to increase the sealing between the top cover 4 and the outer wall 6 of the heating cup, reduce gas leakage, and prevent bacterial endotoxins in the outside air from contaminating the sample solution.

[0039] The top surface of the heating base 1 is also covered with an asbestos layer 15. The asbestos layer 15 has the functions of heat insulation and uniform heat conduction, preventing the sample heating cup 3 from cracking due to uneven heating.

[0040] The heating base 1 is also equipped with a temperature control panel 2 on its side, which can control the heating temperature. The optimal heating temperature of the sample solution can be achieved through the temperature control panel 2.

[0041] In summary, the operating principle of this device is as follows: The sample heating cup 3 is made of high-temperature glass, which allows for dry heat removal of the heat source, reducing contamination during sample solution pretreatment; a potassium dichromate-sulfuric acid solution is used as the ethanol absorbent because ethanol is largely absorbed in the potassium dichromate solution. During absorption, high-valent chromium oxidizes the absorbed ethanol, turning the orange-red solution yellow-green; the concentrated sulfuric acid in the absorbent provides an acidic environment, enhancing the oxidizing power of high-valent chromium; the height of the removable annular cup 5 is at least 0.5 cm lower than the outer wall 6 of the heating cup, allowing the top cover 4 to seal the entire sample heating cup 3 while allowing ethanol gas escaping from the inner cup 14 to enter the removable annular cup 5; the contact surface between the top of the outer wall 6 of the heating cup and the top cover 4 is frosted, increasing the airtightness between the top cover 4 and the sample heating cup 3; and the temperature is adjusted... The operation panel 2 controls the temperature according to the heat resistance of the sample, thus minimizing the time while ensuring the removal of ethanol solution. An asbestos layer 15 is provided between the sample heating cup 3 and the heating base 1 to prevent damage to the sample heating cup 3 due to uneven heating. Since the height of the inner cup 14 is at least 1 cm lower than that of the removable annular cup 5, the removable annular cup 5 is higher than the nearby contact surface in the sample heating cup 3, and can be picked up with tweezers. The annular inner wall 11 of the removable annular cup 5 has a double-layer glass structure with a pure white coating between the two layers. This is to eliminate the interference of sample color in the background after adding potassium dichromate-sulfuric acid solution, making it easier to observe its color change. The ethanol absorption liquid level should be 0.5-1.5 cm. Too little absorption liquid requires frequent replacement, increasing the risk of contamination, while too much absorption liquid will cause waste.

[0042] By adding an ethanol absorbent and heating it, the saturated vapor pressure of ethanol is altered, accelerating ethanol evaporation and effectively shortening the heating time, thus increasing experimental efficiency. Furthermore, observing the color change of the ethanol absorbent in the heating cup allows for assessment of ethanol removal from the sample, further reducing interference from the sample to the Limulus Amebocyte Lysate (LAL) gel reaction. The sealed sample heating cup 3, combined with a shorter heating time, reduces the time the sample solution is exposed to air, preventing contamination by airborne bacterial endotoxins and increasing experimental accuracy.

[0043] In summary, the method of using this device is as follows: Before use, the sample heating cup 3 is subjected to dry heat treatment to remove the heat source. After the sample heating cup 3 is in a heat-free state, the sample to be treated is added to the inner cup 14. Then, potassium dichromate-sulfuric acid solution is added to the removable annular cup 5 as the ethanol absorption solution 16 and the indicator solution. Afterwards, the sealing surface 13 at the top of the outer wall 6 of the heating cup is gently rubbed against the top cover 4 and then closed. The heating base 1 is opened, and the heating temperature is selected according to the sample to be treated. During heating, the color change of the ethanol absorption solution in the removable annular cup 5 is observed. After it turns completely yellow-green for 5 minutes, the top cover 4 is opened, and the annular inner wall 11 of the removable annular cup 5 is grasped with tweezers without a heat source and removed. Then, the ethanol absorption solution is replaced, and the above process is repeated until the ethanol absorption solution no longer changes color, indicating that the sample treatment is complete and the sample can be removed for the bacterial endotoxin gel examination.

[0044] The present invention discloses a pretreatment device for ethanol in a sample. An ethanol-containing sample solution is added to an inner cup, and an ethanol absorption liquid is added to a first receiving cavity. The top cover is then closed to seal the sample heating cup. The ethanol that evaporates upon heating is absorbed by the ethanol absorption liquid. By absorbing ethanol within the sealed space, not only is the ethanol evaporation efficiency and absorption effect improved, but contamination of the sample solution by external substances is also avoided.

[0045] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A pretreatment device for ethanol components in a sample, comprising a heating base (1) on which a sample heating cup (3) is placed, characterized in that, The sample heating cup (3) includes a bottom (7), an inner wall (8), and an outer wall (6). The inner wall (8) and the outer wall (6) are both fused to the bottom (7) of the heating cup, and the cross-sections of the inner wall (8) and the outer wall (6) are arranged in a concentric ring from the inside to the outside. The height of the inner wall (8) of the heating cup is lower than the height of the outer wall (6) of the heating cup, and the outer wall (6) of the heating cup is covered with a top cover (4) that can seal the internal cavity of the outer wall (6) of the heating cup. The inner wall (8) and the bottom (7) of the heating cup form an inner cup (14) that can hold the sample solution; the inner wall (8), the outer wall (6) and the bottom (7) of the heating cup form a first receiving cavity (12) that can hold the ethanol absorption liquid.

2. The pretreatment device for ethanol components in a sample according to claim 1, characterized in that, It also includes a removable annular cup (5) placed in the first receiving cavity (12); the removable annular cup (5) includes an annular bottom surface (9), an annular outer wall (10) and an annular inner wall (11), the annular outer wall (10) and the annular inner wall (11) are both fused to the annular bottom surface (9); the annular outer wall (10), the annular bottom surface (9) and the annular inner wall (11) form a second receiving cavity (16) that can contain ethanol absorption liquid.

3. The pretreatment device for ethanol components in a sample according to claim 2, characterized in that, The height of the removable ring cup (5) is less than the height of the outer wall (6) of the heating cup.

4. The pretreatment device for ethanol components in a sample according to claim 3, characterized in that, The height of the annular inner wall (11) is greater than the height of the inner wall (8) of the heating cup.

5. The pretreatment device for ethanol components in a sample according to claim 4, characterized in that, An operating gap is reserved between the annular inner wall (11) and the inner wall (8) of the heating cup.

6. The pretreatment device for ethanol components in a sample according to claim 2, characterized in that, The second receiving cavity (16) has at least one inner wall with a pure white coating.

7. A pretreatment apparatus for ethanol components in a sample according to any one of claims 1 to 5, characterized in that, The sealing surface (13) of the top of the outer wall (6) of the heating cup, which can contact the top cover (4), is a frosted surface.

8. A pretreatment apparatus for ethanol components in a sample according to any one of claims 1 to 5, characterized in that, The top surface of the heating base (1) is also covered with an asbestos layer (15).

9. A pretreatment apparatus for ethanol components in a sample according to any one of claims 1 to 5, characterized in that, The heating base (1) is also provided with a temperature control panel (2) on its side, which can control the heating temperature.

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