Liquid biological product virus inactivation device

Abstract

The utility model relates to a kind of liquid biological product virus inactivation device, it includes dust-free box and the horizontal one-way conveying caterpillar being set in dust-free box inside, upper sample unit, light shielding unit and ultraviolet irradiation unit are sequentially set in the above of conveying caterpillar along the conveying direction of conveying caterpillar, collection unit and cleaning unit are sequentially set in the below of conveying caterpillar along the direction opposite to the conveying direction of conveying caterpillar, upper sample unit includes drop liquid device for adding liquid biological product to the upper surface of conveying caterpillar, light shielding unit includes light shield, ultraviolet irradiation unit includes ultraviolet lamp, collection unit includes first material collecting groove and the scraping strip being set in first material collecting groove, scraping strip is contacted with the lower surface of conveying caterpillar, cleaning unit includes flushing device, drying device and cooling device.The utility model can provide stable virus inactivation efficiency and biological activity retention when long time operation, realize the continuous automation virus inactivation of large-scale liquid biological product truly.

Description

Technical Field

[0001] This utility model relates to the technical field of virus inactivation devices, specifically to a virus inactivation device for liquid biological products. Background Technology

[0002] With the development and expansion of the use of various biological products in the biotechnology industry, the risk of animal-derived viral infection has become increasingly prominent. Virus inactivation is a crucial step in the preparation of liquid biological products, aiming to kill viruses without affecting the function of the liquid biological products.

[0003] There are various methods for inactivating viruses in liquid biological products, mainly including physical, chemical, and biochemical methods. Physical methods include heat inactivation, ultraviolet inactivation, and membrane filtration; chemical methods include organic solvent / detergent treatment, formaldehyde and β-propiolactone (BPL) inactivation, and peracetic acid inactivation; and biochemical methods include pH adjustment and enzyme treatment. Other methods include photochemical methods (such as methylene blue photochemical inactivation), ozone inactivation, and ultrasonic inactivation.

[0004] Ultraviolet (UV) inactivation is a simple, rapid, broad-spectrum, efficient, and non-contaminating method for virus inactivation. It directly targets viral nucleic acids, disrupting their structure and inhibiting replication. Applicable to various viruses, including enveloped and non-enveloped viruses, it has significant application value in the field of virus inactivation in liquid biological products. To simultaneously ensure the bioactivity of liquid biological products and the effectiveness of virus inactivation, specific equipment is required. Existing technologies utilize customized containers, allowing the liquid biological product to be continuously introduced and irradiated with UV light. Furthermore, existing technologies use external forces such as centrifugation to thin the liquid layer, ensuring sufficient and effective UV irradiation to increase UV penetration and effectively inactivate viruses. However, during the processing of large quantities of liquid biological samples, some liquid biological products inevitably denature and adhere to the container walls, causing parameter changes and altering the liquid flow pattern. Moreover, the inability to clean the samples frequently during use can negatively impact the UV dose received by the liquid biological product and the retention of sample activity over extended periods. Summary of the Invention

[0005] The purpose of this invention is to provide a novel virus inactivation device for liquid biological products, which can provide stable virus inactivation efficiency and biological activity retention during long-term operation, truly realizing continuous and automated virus inactivation of large-scale liquid biological products.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] A liquid biological product virus inactivation device includes a cleanroom and a horizontal unidirectional conveyor belt disposed inside the cleanroom. A sample loading unit, a light-shielding unit, and an ultraviolet irradiation unit are sequentially arranged above the conveyor belt along the conveying direction of the conveyor belt. A collection unit and a cleaning unit are sequentially arranged below the conveyor belt along the opposite direction of the conveyor belt.

[0008] Specifically, the surface of the conveyor belt is provided with an anti-protein adsorption coating. This coating is non-toxic, harmless, chemically stable, hydrophobic, and anti-protein sticky, and has no effect on liquid biological products and is easy to clean.

[0009] In some embodiments, the anti-protein adsorption coating is formed from one or more of fluorinated silanes, PEG (polyethylene glycol), and zwitterionic polymers (such as polysulfobetaine). The anti-protein adsorption coating can be applied to the track surface directly from the above substances or through plasma treatment or chemical modification. The coating thickness does not exceed 5 μm.

[0010] Specifically, the sample loading unit includes a dripping device for adding liquid biological products to the upper surface of the conveyor belt. The liquid biological products are placed in droplets on the upper surface of the conveyor belt via the dripping device, and then transported by the conveyor belt through an ultraviolet irradiation unit for ultraviolet sterilization. The droplets are thin, the operation is simple, and it can quickly and effectively solve the problem of light's inability to penetrate thick liquids.

[0011] Specifically, the light-shielding unit includes a light-shielding plate that separates the sample loading unit from the ultraviolet irradiation unit. The light-shielding plate can block ultraviolet light emitted from the ultraviolet region, and to the greatest extent possible, block the light from the ultraviolet irradiation unit from shining on the opening of the dripping device, preventing the liquid biological product from denaturing and adhering at the opening, thereby preventing blockage of the dripping device opening and uneven flow rate.

[0012] Specifically, the ultraviolet irradiation unit includes one or more ultraviolet lamps, and an appropriate number of ultraviolet lamps can be selected and installed according to actual needs.

[0013] Specifically, the collection unit includes a first receiving trough and a scraper disposed in the first receiving trough, the scraper contacting the lower surface of the conveyor belt. Since the conveyor belt is unidirectional, after it tumbles down, the virus-inactivated liquid biological product will have a downward dripping tendency under the influence of gravity, and will naturally drip into the first receiving trough when it encounters the scraper.

[0014] Specifically, the cleaning unit includes a rinsing device, a drying device, and a cooling device. The rinsing device is used to rinse away any residual liquid biological products on the lower surface of the conveyor belt; the drying device is used to dry the cleaned portion of the conveyor belt; and the cooling device is used to cool the dried portion of the conveyor belt to prevent excessively high local temperatures that could cause subsequent application of liquid biological products to that local surface to deform or become inactive.

[0015] In some embodiments, the dripping device includes a sample holder, which is parallel to the conveyor belt and extends in a direction perpendicular to the conveying direction of the conveyor belt. The sample holder has a storage bin inside, and one or more feed pipes connected to the storage bin are provided at the top of the sample holder. The bottom of the sample holder has multiple dripping ports connected to the storage bin, and the multiple dripping ports are arranged sequentially at intervals along the extension direction of the sample holder.

[0016] Specifically, the dripping device further includes a pump located outside the cleanroom and connected to the feed pipe. The pump is used to continuously feed the liquid biological product to be inactivated into the sample rack. The pump can be a pump existing in the art, such as a peristaltic pump or a diaphragm pump.

[0017] Preferably, the sample holder is made of an opaque material, and more preferably a material that has no effect on liquid biological products and is easy to clean.

[0018] In some embodiments, the dripping device is movably disposed in the cleanroom along a conveying direction parallel to the conveyor belt, facilitating adjustment of the sample loading site.

[0019] In some embodiments, there is a gap between the lower end of the light shield and the upper surface of the conveyor belt. Preferably, the lower end of the light shield is as close as possible to the upper surface of the conveyor belt, so as to avoid ultraviolet light irradiating the sample holder without affecting the operation of the conveyor belt.

[0020] In some embodiments, the light-shielding plate is perpendicular to the horizontal plane and extends from one side of the conveyor belt to the other side, effectively ensuring the ultraviolet light blocking effect.

[0021] In some embodiments, the light-shielding plate is movably disposed in the cleanroom in the vertical direction, which facilitates the vertical adjustment of the light-shielding plate and the maintenance of the device.

[0022] In some embodiments, the light-shielding plate is movably disposed in the cleanroom along the conveying direction parallel to the conveyor belt, so as to facilitate timely adjustment of the position of the light-shielding plate on the conveyor belt according to the setting of the sample rack and the ultraviolet irradiation area.

[0023] In some embodiments, the ultraviolet lamp is parallel to the horizontal plane and extends in a direction perpendicular to the conveyor belt's conveying direction, which helps to improve the ultraviolet irradiation effect.

[0024] In some embodiments, the plurality of ultraviolet lamps are arranged sequentially at intervals along the conveyor belt's conveying direction, so that the ultraviolet light received on the upper surface of the conveyor belt in the ultraviolet irradiation area is as uniform as possible.

[0025] In some embodiments, the ultraviolet lamp can be moved vertically within the cleanroom to facilitate adjustment of the irradiation dose as needed and to facilitate subsequent maintenance.

[0026] In some embodiments, the ultraviolet irradiation unit is further equipped with a reflective light shield, with the portion of the upper surface of the conveyor belt located within the light shield and the ultraviolet lamp both situated inside the light shield. This blocks ultraviolet rays from propagating vertically from top to bottom without interfering with other units. Combined with the use of a light shield plate, this further ensures that the sample loading unit is not affected by ultraviolet light. The light shield has a reflective coating.

[0027] In some embodiments, the wavelength of the ultraviolet lamp is 240-280nm, such as 240nm, 241nm, 242nm, 243nm, 244nm, 245nm, 246nm, 247nm, 248nm, 249nm, 250nm, 251nm, 252nm, 253nm, 254nm, 255nm, 256nm, 257nm, 258nm, 259nm, 260nm, etc. Any wavelength between any two specific wavelengths, including 261nm, 262nm, 263nm, 264nm, 265nm, 266nm, 267nm, 268nm, 269nm, 270nm, 271nm, 272nm, 273nm, 274nm, 275nm, 276nm, 277nm, 278nm, 279nm, 280nm, or above, can be selected according to actual needs.

[0028] In some embodiments, the minimum vertical distance between the ultraviolet lamp and the upper surface of the conveyor belt is 1-10cm, such as any distance between any two specific distances of 1cm, 2cm, 3cm, 4cm, 5cm, 6cm, 7cm, 8cm, 9cm, 10cm or more, and an appropriate distance can be selected according to the actual required irradiation energy.

[0029] In some embodiments, the power of the ultraviolet lamp is 10-30W, for example, any power between any two specific power values ​​of 10W, 11W, 12W, 13W, 14W, 15W, 16W, 17W, 18W, 19W, 20W, 21W, 22W, 23W, 24W, 25W, 26W, 27W, 28W, 29W, 30W, or above. The appropriate power can be selected according to the actual required irradiation energy.

[0030] In some implementations, the number of ultraviolet lamps is 1-10, for example, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, and an appropriate number of ultraviolet lamps are selected or turned on according to actual needs.

[0031] When there are multiple UV lamps, it is preferable that the distance between two adjacent UV lamps is 5-10cm, such as any distance between any two specific distances of 5cm, 6cm, 7cm, 8cm, 9cm, 10cm or more. The appropriate distance can be selected according to the actual required irradiation energy.

[0032] In some embodiments, the first receiving trough is located below the distal end of the conveyor belt, parallel to the conveyor belt and extending perpendicular to the conveying direction of the conveyor belt. Preferably, the horizontal projection of the distal end of the conveyor belt falls inside and slightly rearward of the first receiving trough, and the scraper is located in the middle of the first receiving trough to minimize the leakage of liquid biological products after virus inactivation.

[0033] In some embodiments, the two ends of the first receiving trough extend beyond the sides of the conveyor belt to further prevent the leakage of liquid biological products after virus inactivation.

[0034] Specifically, the bottom of the first receiving trough is connected to a storage device located below it or outside the cleanroom via a pipe.

[0035] In some embodiments, the scraper is located in the middle of the first receiving trough, and the scraper extends from one side of the conveyor belt to the other side, further preventing the leakage of liquid biological products after virus inactivation.

[0036] The material of the first receiving trough is not specifically limited. It can be made of a material that is corrosion-resistant, stable, and biocompatible in the field, such as glass, polymer, or metal.

[0037] Preferably, the scraper is made of a flexible solid material to avoid damaging the coating on the surface of the conveyor belt. For example, silicone is chosen because it has advantages such as strong adhesion, low hardness that does not damage the coating, compliance with GMP requirements for material contact, non-toxicity and good biocompatibility, easy shaping, replaceability, and moderate cost.

[0038] In some embodiments, the scraper is fixedly connected to the inside of the first receiving trough via a bracket. The material of the bracket is not specifically limited, and any material that is corrosion-resistant, stable, and biocompatible in the art can be used, and may be the same as or different from the first receiving trough.

[0039] In some embodiments, the collection unit further includes a first spraying device for spraying buffer solution onto the conveyor belt and a second collection trough for collecting the buffer solution. A small amount of buffer solution can be used to rinse the surface of the conveyor belt, flushing the active ingredients of any remaining liquid biological product into the second collection trough for collection. After collection, the solution can be concentrated and reused.

[0040] Specifically, the first spraying device is connected via a pipe to a buffer solution storage container located below the second receiving trough or outside the cleanroom.

[0041] In some embodiments, one or more of the first spraying devices are provided along the extension direction of the second receiving trough, or the first spraying devices are connected to a plurality of spray heads via pipes, the plurality of spray heads being spaced apart along the extension direction of the second receiving trough.

[0042] Specifically, the bottom of the second receiving trough is connected via a pipe to a buffer solution receiving container located below it or outside the cleanroom.

[0043] Furthermore, the second receiving trough is located between the first receiving trough and the rinsing device.

[0044] Furthermore, the second receiving trough is parallel to the conveyor belt and extends in a direction perpendicular to the conveyor belt's conveying direction, in order to collect as much buffer solution containing biological products as possible.

[0045] Furthermore, the two ends of the second receiving trough extend beyond the sides of the conveyor belt to collect buffer solutions containing biological products as much as possible.

[0046] Furthermore, the material of the second receiving trough is not specifically limited, and any material that is corrosion-resistant, stable, and biocompatible in the field can be used, such as glass, polymer, or metal, which may be the same as or different from the first receiving trough.

[0047] Furthermore, the first spraying device is located above one end of the second receiving trough, and its spraying direction is upward and toward the center of the conveyor belt.

[0048] Furthermore, the second spraying device is located on the outside and below one side of the conveyor belt, and it is fixedly installed in the cleanroom by a fixing bracket.

[0049] In some embodiments, the rinsing device includes a second spraying device for spraying cleaning fluid onto the conveying track and a collection tank for collecting the cleaning fluid.

[0050] Specifically, the cleaning solution is preferably pure water. Rinsing the surface of the conveyor belt with an appropriate amount of pure water further ensures that there are no liquid biological product residues, while preventing ion residues in the buffer solution.

[0051] In some embodiments, the liquid collection tank is parallel to the conveyor belt and extends perpendicular to the conveyor belt's conveying direction, in order to collect as much cleaning liquid as possible.

[0052] In some embodiments, the two ends of the liquid collection tank extend beyond the sides of the conveyor belt to collect as much cleaning fluid as possible.

[0053] In some embodiments, the second spraying device is connected via a pipe to a cleaning fluid storage container located below the collection tank or outside the cleanroom.

[0054] In some embodiments, the bottom of the collection tank is connected via a pipe to a waste liquid container located below it or outside the cleanroom.

[0055] In some embodiments, one or more of the second spraying devices are provided along the extension direction of the liquid collection tank, or the second spraying devices are connected to a plurality of spray heads through pipes, the plurality of spray heads being spaced apart along the extension direction of the liquid collection tank.

[0056] Furthermore, the material of the liquid collection tank is not specifically limited; any corrosion-resistant material in the art can be used.

[0057] In some embodiments, the second spraying device is located above one end of the liquid collection tank, with its spraying direction upward and toward the center of the conveyor belt.

[0058] Furthermore, the second spraying device is located on the outside of the conveyor belt and is fixedly installed in the cleanroom by a fixing bracket.

[0059] In some embodiments, the drying device includes an electric heating wire and a first fan for removing cleaning fluid from the surface of the conveyor belt.

[0060] In some embodiments, the cooling device includes a water-cooled pipe and a second fan. The water-cooled pipe is connected to a cold water circulation system outside the cleanroom to reduce the surface temperature of the dried conveyor belt to room temperature or below, so as to prevent the local high temperature on the surface of the conveyor belt from affecting the bioactivity of the liquid biological products during subsequent cycles.

[0061] In some embodiments, the conveyor belt includes a frame, a drive unit, a transmission unit, and a track.

[0062] Specifically, the frame includes a horizontal support plate and a support frame, with the upper end of the support frame fixedly connected to the horizontal support plate. The drive device includes a motor mounted on the horizontal support plate. The transmission device includes a drive roller disposed at one end of the horizontal support plate and a driven roller disposed at the other end of the horizontal support plate. The drive roller is connected to the motor, and the motor can drive the drive roller to rotate. The track is sleeved on the drive roller, the horizontal support plate, and the driven roller, and the track is in a taut state with both its upper and lower surfaces parallel to the horizontal plane.

[0063] Furthermore, the material of the track is not specifically limited, including but not limited to silicone rubber, polytetrafluoroethylene or polyurethane.

[0064] In some specific embodiments, the horizontal tray is provided with a heat insulation film, and at least the lower surface of the horizontal tray is covered with the heat insulation film.

[0065] In some embodiments, the liquid biological product virus inactivation device further includes a control console and a power module disposed outside the cleanroom. The power module is used to supply power to the equipment inside the cleanroom, and the control console is electrically connected to the equipment inside the cleanroom to control the operation of the equipment inside the cleanroom, thereby achieving a high degree of automation.

[0066] In some embodiments, the cleanroom is provided with a viewing window to facilitate observation of the operation of the various devices inside the cleanroom.

[0067] In some implementations, the cleanroom is removable for maintenance.

[0068] In some embodiments, the cleanroom is equipped with a temperature sensor.

[0069] Specifically, the temperature sensor is electrically connected to the control console. The temperature sensor is used to monitor the temperature inside the cleanroom. When the temperature exceeds a preset temperature threshold, the control console will control the cooling device to improve the cooling efficiency and prevent the temperature inside the cleanroom from being too high, which could affect the biological activity of the liquid biological products.

[0070] In some embodiments, the cleanroom is equipped with an ultraviolet radiometer and a hygrometer, which are electrically connected to the control console.

[0071] Furthermore, the console is equipped with a control panel, which includes a display screen that can display the temperature, humidity and ultraviolet irradiance inside the box, as well as operation buttons for setting parameters such as conveyor belt speed, temperature, humidity and ultraviolet irradiance inside the box.

[0072] In some specific embodiments, the bottom of the cleanroom is provided with a track parallel to the conveyor belt's conveying direction. A first retractable support, a second retractable support, and a third retractable support, each vertically extendable, are slidably arranged on the track along the conveyor belt's conveying direction. The dripping device is connected to the upper part of the first retractable support, the light-shielding plate is connected to the upper part of the second retractable support, and the ultraviolet lamp is connected to the upper part of the third retractable support. Preferably, when there are multiple ultraviolet lamps, the number of third retractable supports is equal to the number of ultraviolet lamps, with each ultraviolet lamp corresponding to one third retractable support. The first retractable support, the second retractable support, and the third retractable support are electrically connected to the control console, which allows control of the movement and extension of the first retractable support, the second retractable support, and the third retractable support.

[0073] Due to the application of the above technical solution, this utility model has the following advantages compared with the prior art:

[0074] This invention relates to a liquid biological product virus inactivation device that transports liquid biological products as droplets via a conveyor belt. During transport, the products are irradiated with ultraviolet light, effectively solving the problem of uneven virus inactivation caused by the difficulty of light penetrating thick liquids. The planar transport and porous droplet design of the conveyor belt ensure that the liquid biological products always move in the same pattern and thickness on the irradiated plane, resulting in more stable dosage. A collection and cleaning unit located below the conveyor belt automatically collects the virus-inactivated liquid biological products and automatically cleans the conveyor belt, avoiding the problem of residual denaturation of the liquid biological products affecting subsequent inactivation effects. This allows for continuous and efficient virus inactivation while maximizing the preservation of the biological activity of the liquid biological products. During long-term operation, it provides stable virus inactivation efficiency and biological activity retention, truly achieving large-scale continuous automated virus inactivation of liquid biological products. Attached Figure Description

[0075] Figure 1 This is a simplified schematic diagram of the overall structure of the liquid biological product virus inactivation device in Example 1.

[0076] Figure 2 This is a simplified schematic diagram of part of the main structure of the liquid biological product virus inactivation device located inside the cleanroom in Example 1.

[0077] In the attached diagrams above, 1 is a cleanroom; 2 is a conveyor belt; 211 is a horizontal pallet; 212 is a support frame; 22 is a motor; 23 is a driven roller; 24 is a conveyor belt; 3 is a sample loading unit; 31 is a sample loading rack; 4 is a light-shielding unit; 41 is a light-shielding plate; 5 is a UV irradiation unit; 51 is a UV lamp; 6 is a collection unit; 61 is a first receiving trough; 62 is a scraper; 63 is a bracket; 64 is a first spraying device; 65 is a second receiving trough; 7 is a cleaning unit; 71 is a second spraying device; 72 is a liquid collection trough; 73 is an electric heating wire; 74 is a first fan; 75 is a water-cooling pipe; 76 is a second fan; and 8 is a control console. Detailed Implementation

[0078] The present invention will be further described below with reference to the embodiments shown in the accompanying drawings.

[0079] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0080] In the description of this utility model, it should be noted that the directional terms such as "upper" and "lower" are all used in accordance with... Figure 2 The terms "inner" and "outer" are defined by their distance from the center of the device or component, with "inner" being the position closer to the center and "outer" being the position farther from the center. "Proximal end" refers to the end closer to the dripping device, and "distal end" refers to the end farther from the dripping device. "Transmission direction" refers to the direction in which material is transported from the "proximal end" to the "distal end" on the upper surface of the conveyor belt. These directional terms are used only for the convenience of describing this utility model and for simplifying the description, and are not intended to indicate or imply that the device or component must have a specific orientation, or be constructed and operated in a specific orientation; therefore, they should not be construed as limitations on this utility model.

[0081] In the description of this utility model, it should be noted that when described as above the conveyor belt, it means above the upper surface of the conveyor belt; when described as below the conveyor belt, it means below the lower surface of the conveyor belt.

[0082] In this embodiment of the invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can be a direct connection or an indirect connection through an intermediate component. Those skilled in the art can understand the specific meaning of the above terms in this embodiment of the invention according to the specific circumstances.

[0083] The accompanying drawings provided in this utility model are merely schematic diagrams of the structure of the virus inactivation device for liquid biological products, intended to facilitate the reader's understanding, and do not represent a limitation on the structure of the virus inactivation device for liquid biological products.

[0084] To simplify the disclosure of embodiments of this utility model, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the embodiments of this utility model. Specifications and models of components can be adjusted according to actual needs.

[0085] Example 1

[0086] like Figure 1 and Figure 2 As shown, this embodiment provides a liquid biological product virus inactivation device, which includes a cleanroom box 1 and a horizontal unidirectional conveyor belt 2 disposed inside the cleanroom box 1. A sample loading unit 3, a light-shielding unit 4, and an ultraviolet irradiation unit 5 are sequentially arranged above the conveyor belt 2 along the conveying direction of the conveyor belt 2. A collection unit 6 and a cleaning unit 7 are sequentially arranged below the conveyor belt 2 in the opposite direction to the conveying direction of the conveyor belt 2.

[0087] Specifically, the top and sides of the cleanroom 1 can be opened. The cleanroom 1 is equipped with a viewing window for easy observation of the operation of the equipment inside. The cleanroom 1 is removable for maintenance. In this embodiment, the cleanroom 1 has a width of 4m and a height of 2m; in other embodiments, the dimensions can be adjusted according to the internal equipment.

[0088] Specifically, the conveyor belt 2 includes a frame, a drive unit, a transmission unit, and a track 24. In this embodiment, the frame includes a horizontal support plate 211 and a support frame 212, with the upper end of the support frame 212 fixedly connected to the horizontal support plate 211. The drive unit includes a motor 22 mounted on the horizontal support plate 211. The transmission unit includes a drive roller disposed at one end of the horizontal support plate 211 and a driven roller 23 disposed at the other end of the horizontal support plate 211. The drive roller is connected to the motor 22, and the motor 22 can drive the drive roller to rotate. The track 24 is fitted onto the drive roller, the horizontal support plate 211, and the driven roller 23, and the track 24 is in a tensioned state with both its upper and lower surfaces parallel to the horizontal plane. In this embodiment, the track 24 is made of rubber, and its surface is coated with an anti-protein adsorption coating. The main component of this coating is fluorinated silane, which is non-toxic, harmless, chemically stable, water-resistant, has no effect on liquid biological products, and is easy to clean. In other embodiments, polyethylene glycol coatings, polysulfobetaine coatings, etc., can also be used, as long as they can achieve anti-protein adsorption and have a certain degree of hydrophobicity. The material of the horizontal pallet 211 is not limited and can be metal, stainless steel, or polymer material. The lower surface of the horizontal pallet 211 is covered with a heat insulation film. In this embodiment, the width of the track 24 is 1m, the total length of the track 24 is 8m, the diameter of the driving wheel and the driven wheel are 0.2m respectively, and the motor 22 drives the track 24 to rotate counterclockwise. In other embodiments, other specifications of conveyor tracks 2 can be selected according to actual needs or the size of the cleanroom 1.

[0089] Specifically, the sample loading unit 3 includes a dripping device for dripping liquid biological products onto the upper surface of the conveyor belt 2. The liquid biological products are placed on the upper surface of the conveyor belt 2 in the form of droplets, and then conveyed by the conveyor belt 2 through the ultraviolet irradiation unit 5 for ultraviolet sterilization. The droplet thickness is small, the operation is simple, and it can quickly and effectively solve the problem that light cannot penetrate thick liquids. At the same time, the planar conveying of the conveyor belt and the porous dripping scheme can ensure that the liquid biological products always run in the irradiated plane with the same pattern and thickness, resulting in more stable dosage. In this embodiment, the dripping device includes a sample mounting frame 31, which is parallel to the conveyor belt 2 and extends perpendicular to the conveying direction of the conveyor belt 2. The sample mounting frame 31 is provided with a storage bin, and the top of the sample mounting frame 31 is provided with one or more feed pipes connected to the storage bin. The bottom of the sample mounting frame 31 has multiple dripping ports connected to the storage bin, and the multiple dripping ports are arranged sequentially at intervals along the extension direction of the sample mounting frame 31. In this embodiment, the dripping device also includes a peristaltic pump (not shown in the figure) connected to the feed pipe and disposed outside the cleanroom 1. The peristaltic pump is used to continuously feed the liquid biological product to be inactivated into the sample rack 31. The sample rack 31 is made of quartz, which has no effect on the liquid biological product and is easy to clean. The dripping device is movably disposed in the cleanroom 1 along the conveying direction parallel to the conveyor belt 2, facilitating the adjustment of the sample loading point.

[0090] Specifically, the light-shielding unit 4 includes a light-shielding plate 41 that separates the sample loading unit 3 from the ultraviolet irradiation unit 5. The light-shielding plate 41 can block ultraviolet light emitted from the ultraviolet region, maximally blocking the light from the ultraviolet irradiation unit 5 from irradiating the opening of the dripping device, preventing the liquid biological product at the opening from denaturing and adhering, thereby preventing blockage of the dripping device opening and uneven flow rate. In this embodiment, there is a gap between the lower end of the light-shielding plate 41 and the upper surface of the conveyor belt 2. The lower end of the light-shielding plate 41 is as close as possible to the upper surface of the conveyor belt 2, so as to avoid ultraviolet light irradiating the sample loading rack 31 as much as possible without affecting the operation of the conveyor belt 2. The light-shielding plate 41 is perpendicular to the horizontal plane and extends from one side of the conveyor belt 2 to the other side. The light-shielding plate 41 is movably installed in the cleanroom 1 in the vertical direction, which facilitates the vertical adjustment of the light-shielding plate 41 and the maintenance of the device. The light shield 41 is movably installed in the cleanroom 1 along the conveying direction parallel to the conveyor belt 2, so that the position of the light shield 41 on the conveyor belt 2 can be adjusted in a timely manner according to the setting of the sample rack 31 and the ultraviolet irradiation area.

[0091] Specifically, the ultraviolet irradiation unit 5 includes one or more ultraviolet lamps 51, and an appropriate number of ultraviolet lamps 51 can be selected and installed according to actual needs. In this embodiment, multiple ultraviolet lamps 51 are provided, each of which is parallel to the horizontal plane and extends in a direction perpendicular to the conveyor belt 2, which is beneficial to improving the ultraviolet irradiation effect. The multiple ultraviolet lamps 51 are arranged sequentially and at intervals along the conveyor belt, so that the ultraviolet light received by the upper surface of the conveyor belt 2 in the ultraviolet irradiation area is as uniform as possible. The ultraviolet lamps 51 can be moved in the cleanroom 1 in the vertical direction, which is convenient for adjusting the irradiation dose according to actual needs and for subsequent maintenance. In this embodiment, the ultraviolet irradiation unit 5 is also provided with a reflective light shield, and the part of the upper surface of the conveyor belt 2 located in the light shielding unit 4 and the ultraviolet lamps 51 are all located inside the light shield. It can block ultraviolet rays so that they propagate vertically from top to bottom without interfering with other units. With the use of the light shield 41, it will further ensure that the sample loading unit 3 is not affected by ultraviolet light. The light shield has a reflective coating.

[0092] In this embodiment, the wavelength of the ultraviolet lamp 51 is 253.7 nm. In other embodiments, the wavelength of the ultraviolet lamp 51 can be adjusted according to actual needs, preferably within the range of 240-280 nm. In this embodiment, the vertical distance between the ultraviolet lamp 51 and the upper surface of the conveyor belt 2 is adjustable from 1 to 10 cm, and the power of the ultraviolet lamp 51 is 20 W. In other embodiments, an ultraviolet lamp 51 with a suitable power can be selected according to actual needs.

[0093] Specifically, the collection unit 6 includes a first receiving trough 61 and a scraper 62 disposed in the first receiving trough 61, the scraper 62 contacting the lower surface of the conveyor belt 2. Since the conveyor belt 2 is unidirectional, after it tumbles down, the virus-inactivated liquid biological product will tend to drip downwards under the influence of gravity, and will naturally drip into the first receiving trough 61 upon encountering the scraper 62. In this embodiment, the first receiving trough 61 is located below the far end of the conveyor belt 2, parallel to the conveyor belt 2 and extending perpendicular to the conveying direction of the conveyor belt 2. More specifically, the horizontal projection of the far end of the conveyor belt 2 falls inside the first receiving trough 61 and is slightly rearward, while the scraper 62 is located in the middle of the first receiving trough 61, minimizing the possibility of missed collection of the virus-inactivated liquid biological product. The two ends of the first receiving trough 61 extend beyond the sides of the conveyor belt 2, further preventing the missed collection of the virus-inactivated liquid biological product. The bottom of the first receiving trough 61 is connected via a pipe to a storage device (not shown in the figure) located below it or outside the cleanroom 1. A scraper 62 is located in the middle of the first receiving trough 61, extending from one side of the conveyor belt 2 to the other, further preventing the leakage of liquid biological products after virus inactivation. The first receiving trough 61 is made of metal; in other embodiments, a corrosion-resistant, stable, and biocompatible material may also be used. The scraper 62 is made of silicone, which has a certain degree of flexibility to avoid damaging the coating on the surface of the conveyor belt 2. The scraper 62 is fixedly connected to the inside of the first receiving trough 61 via a bracket 63. The bracket 63 is made of the same material as the first receiving trough 61; in other embodiments, a corrosion-resistant, stable, and biocompatible material may also be used.

[0094] In this embodiment, the collection unit 6 further includes a first spraying device for spraying buffer solution onto the conveyor belt 2 and a second receiving trough 65 for collecting the buffer solution. A small amount of buffer solution can be used to rinse the surface of the conveyor belt 2, flushing the effective components of the residual liquid biological product into the second receiving trough 65 for collection. After collection, it can be concentrated and reused. The first spraying device is connected via a pipe to a buffer solution storage container (not shown) located below the second receiving trough 65 or outside the cleanroom 1. Preferably, one or more first spraying devices 64 are arranged along the extension direction of the second receiving trough 65, or the first spraying devices 64 are connected to multiple spray heads via pipes, with the multiple spray heads spaced apart along the extension direction of the second receiving trough 65. In this embodiment, two first spraying devices 64 (the other not shown) are provided, located above both ends of the second receiving trough 65 and on the outer sides of the conveyor belt 2, respectively. They are fixedly installed in the cleanroom 1 by a fixing bracket, with the spraying direction facing upwards and towards the center of the conveyor belt 2. The bottom of the second receiving trough 65 is connected via a pipe to a buffer solution receiving container (not shown in the figure) located below it or outside the cleanroom 1. The second receiving trough 65 is located between the first collecting trough and the rinsing device. The second receiving trough 65 is parallel to the conveyor belt 2 and extends perpendicular to the conveying direction of the conveyor belt 2, collecting buffer solutions containing biological products as much as possible. Both ends of the second receiving trough 65 extend beyond the sides of the conveyor belt 2, collecting buffer solutions containing biological products as much as possible. The material of the second receiving trough 65 is the same as that of the first receiving trough 61. In other embodiments, materials that are corrosion-resistant, stable, and biocompatible in the art can also be used.

[0095] Specifically, the cleaning unit 7 includes a rinsing device, a drying device, and a cooling device. The rinsing device rinses away any residual liquid biological products on the lower surface of the conveyor belt 2; the drying device dries the cleaned portion of the conveyor belt 2; and the cooling device cools the dried portion of the conveyor belt 2 to prevent excessively high local temperatures that could cause subsequent application of liquid biological products to deform or become inactive. In this embodiment, the rinsing device includes a second spraying device for spraying cleaning fluid onto the conveyor belt 2 and a collection tank 72 for collecting the cleaning fluid. The cleaning fluid is preferably pure water. Rinsing the surface of the conveyor belt 2 with an appropriate amount of pure water further ensures that only liquid biological products remain, while preventing the retention of ions from the buffer solution.

[0096] The liquid collection trough 72 is parallel to the conveyor belt 2 and extends perpendicular to the conveying direction of the conveyor belt 2, collecting as much cleaning liquid as possible. The bottom of the liquid collection trough 72 is connected to a waste liquid collection container (not shown in the figure) outside the cleanroom 1 via a pipe. Both ends of the liquid collection trough 72 extend beyond the sides of the conveyor belt 2, collecting as much cleaning liquid as possible. The second spraying device 71 is connected via a pipe to a cleaning liquid storage container (not shown in the figure) located below the second collection trough 65 or outside the cleanroom 1. Preferably, one or more second spraying devices 71 are provided along the extension direction of the liquid collection trough 72, or the second spraying devices 71 are connected to multiple spray heads via pipes, with the multiple spray heads spaced apart along the extension direction of the liquid collection trough 72. In this embodiment, two second spraying devices 71 (the other is not shown) are provided, located above both ends of the liquid collection trough 72 and outside the sides of the conveyor belt 2, and are fixedly installed in the cleanroom 1 by a fixing bracket, with the spraying direction facing upwards and towards the center of the conveyor belt 2. The liquid collection tank 72 is made of the same material as the first material collection tank 61. In other embodiments, a corrosion-resistant material in the art can also be used. In this embodiment, the drying device includes an electric heating wire 73 and a first fan 74 for removing the cleaning fluid from the surface of the conveyor belt 2. The cooling device includes a water-cooling pipe 75 and a second fan 76. The water-cooling pipe 75 is connected to a cold water circulation system outside the cleanroom 1 (not shown in the figure) to reduce the surface temperature of the dried conveyor belt 2 to room temperature or below, so as to prevent the local high temperature on the surface of the conveyor belt 2 from affecting the bioactivity of the liquid biological product during subsequent cycles.

[0097] Specifically, the liquid biological product virus inactivation device also includes a control console 8 and a power module (not shown in the figure) located outside the cleanroom 1. The power module is used to supply power to the equipment inside the cleanroom 1. The control console 8 is electrically connected to the equipment inside the cleanroom 1 to control the operation of the equipment inside the cleanroom 1 and achieve a high degree of automation.

[0098] In this embodiment, a temperature sensor and an ultraviolet radiometer are installed inside the cleanroom 1. The temperature sensor is electrically connected to the control console 8 and is used to monitor the temperature inside the cleanroom 1. When the temperature exceeds a preset temperature threshold, the control console 8 will control the cooling device to improve cooling efficiency and prevent the internal temperature of the cleanroom 1 from becoming too high, which could affect the bioactivity of the liquid biological products. The sensor is also electrically connected to the control console to detect and control the irradiation dose via the control console.

[0099] In this embodiment, the bottom of the cleanroom 1 is provided with a track parallel to the conveyor belt 2. A first retractable support, a second retractable support, and a third retractable support (not shown in the figure) that can be slidably arranged vertically along the conveyor belt 2 are sequentially mounted on the track. A dripping device is connected to the upper part of the first retractable support, a light-shielding plate 41 is connected to the upper part of the second retractable support, and an ultraviolet lamp 51 is connected to the upper part of the third retractable support. Preferably, when there are multiple ultraviolet lamps 51, the number of third retractable supports is equal to the number of ultraviolet lamps 51, with each ultraviolet lamp 51 corresponding to one third retractable support. The first, second, and third retractable supports are electrically connected to the control console 8, which controls the movement and extension of the first, second, and third retractable supports. Further details are omitted here, referring to existing technologies in the field.

[0100] The liquid biological product virus inactivation device of this embodiment can be used for the inactivation of various viruses in various liquid biological products, including but not limited to vaccine preparations, toxin preparations, toxoid preparations, immune sera, blood products, immunoglobulin preparations, antigen preparations, allergen preparations, cytokine preparations, hormone preparations, enzyme preparations, fermentation broths, monoclonal antibody preparations, or in vitro immunodiagnostic products. Viruses include, but are not limited to, one or more of the following families: Reoviridae, Rhabdoviridae, Orthomyxoviridae, Filoviridae, Coronaviridae, Bunyaviridae, Bonaviridae, Flaviviridae, Paramyxoviridae, Clonorviridae, Arenaviridae, Microribonucleoviridae, Caliciviridae, Retroviridae, Poxviridae, Herpesviridae, Iridoviridae, Papillovaviridae, and Parvoviridae.

[0101] In some specific application embodiments of the liquid biological product virus inactivation device in this example, it can drip individually formed droplets of liquid biological product at different speeds without pressure. The dripping speed is adjustable in the range of 6-60 drops / minute per well. Based on a liquid volume of 30-80 μL per drop, the inlet speed is approximately 3.6-384 mL / s. The speed of the conveyor belt is controlled at 0.2-1 cm / s. Within this speed range, the liquid biological product droplets on the surface of the conveyor belt remain stationary relative to the belt surface and do not roll with the belt's movement. The interval between the previous and next droplets of liquid biological product on the same conveyor path is approximately 1 cm. The time for a point on the conveyor belt to pass through a hot air drying zone and a cold air cooling zone of approximately 1 m in length can be controlled within 1-5 minutes. A small amount of the treated liquid biological product is filtered through a 0.22 μm filter for virus titer and biological activity (protein activity) testing. When the virus titer decreases by 4-6 logs, the biological activity retention rate is greater than 90%.

[0102] The above embodiments are only for illustrating the technical concept and features of this utility model, and are intended to enable those skilled in the art to understand the content of this utility model and implement it accordingly. They should not be construed as limiting the scope of protection of this utility model. All equivalent changes or modifications made in accordance with the spirit and essence of this utility model should be included within the scope of protection of this utility model.

Claims

1. A device for inactivating viruses in liquid biological products, characterized in that, It includes a cleanroom and a horizontal unidirectional conveyor belt disposed inside the cleanroom. A sample loading unit, a light-shielding unit, and a UV irradiation unit are sequentially arranged above the conveyor belt along its conveying direction. A collection unit and a cleaning unit are sequentially arranged below the conveyor belt in the opposite direction to its conveying direction. The surface of the conveyor belt is provided with an anti-protein adsorption coating. The sample loading unit includes a dripping device for dripping liquid biological products onto the upper surface of the conveyor belt. The light-shielding unit includes a light-shielding plate that separates the sample loading unit from the ultraviolet irradiation unit. The ultraviolet irradiation unit includes one or more ultraviolet lamps. The collection unit includes a first receiving trough and a scraper blade disposed in the first receiving trough, the scraper blade being in contact with the lower surface of the conveyor belt. The cleaning unit includes a rinsing device, a drying device, and a cooling device.

2. The liquid biological product virus inactivation device according to claim 1, characterized in that, The dripping device includes a sample rack, which is parallel to the conveyor belt and extends perpendicular to the conveying direction of the conveyor belt. The sample rack is provided with a storage bin. The top of the sample rack is provided with one or more feed pipes connected to the storage bin. The bottom of the sample rack is provided with multiple dripping ports connected to the storage bin. The multiple dripping ports are arranged at intervals along the extension direction of the sample rack. And / or, the dripping device is movably disposed in the cleanroom along a conveying direction parallel to the conveyor belt.

3. The liquid biological product virus inactivation device according to claim 1, characterized in that, There is a gap between the lower end of the light-shielding plate and the upper surface of the conveyor belt; And / or, the light shield is perpendicular to the horizontal plane and extends from one side of the conveyor belt to the other side; And / or, the light-shielding plate is movably disposed in the cleanroom in the vertical direction; And / or, the light-shielding plate is movably disposed in the cleanroom along a conveying direction parallel to the conveyor belt.

4. The liquid biological product virus inactivation device according to claim 1, characterized in that, The ultraviolet lamp is parallel to the horizontal plane and extends in a direction perpendicular to the conveyor belt's conveying direction; And / or, the plurality of ultraviolet lamps are arranged sequentially at intervals along the conveyor belt; And / or, the ultraviolet lamp is movable in the cleanroom in the vertical direction; And / or, the ultraviolet irradiation unit is provided with a reflective light shield; And / or, the wavelength of the ultraviolet lamp is 240-280nm; And / or, the minimum vertical distance between the ultraviolet lamp and the upper surface of the conveyor belt is 1-10cm.

5. The liquid biological product virus inactivation device according to claim 1, characterized in that, The first receiving chute is located below the far end of the conveyor belt, and the first receiving chute is parallel to the conveyor belt and extends in a direction perpendicular to the conveying direction of the conveyor belt. And / or, the two ends of the first receiving chute extend beyond the sides of the conveyor belt; And / or, the bottom of the first receiving trough is connected via a pipe to a storage device located below it or outside the cleanroom; And / or, the scraper is disposed in the middle of the first receiving trough, and the scraper extends from one side of the conveyor belt to the other side; And / or, the scraper is made of a flexible solid material.

6. The liquid biological product virus inactivation device according to claim 1, characterized in that, The collection unit also includes a first spraying device for spraying buffer solution onto the conveyor track and a second receiving trough for collecting the buffer solution.

7. The liquid biological product virus inactivation device according to claim 6, characterized in that, The second receiving trough is located between the first receiving trough and the rinsing device; And / or, the second receiving chute is parallel to the conveyor belt and extends in a direction perpendicular to the conveyor belt's conveying direction; And / or, the two ends of the second receiving chute extend beyond the sides of the conveyor belt; And / or, the first spraying device is connected via a pipe to a buffer solution storage container located below the second receiving trough or outside the cleanroom. And / or, the bottom of the second receiving trough is connected via a pipe to a buffer receiving container located below it or outside the cleanroom; And / or, the spraying direction of the first spraying device is upward and toward the near end of the conveyor belt.

8. The liquid biological product virus inactivation device according to claim 1, characterized in that, The rinsing device includes a second spraying device for spraying cleaning fluid onto the conveying track and a collection tank for collecting the cleaning fluid. The liquid collection tank is parallel to the conveyor belt and extends in a direction perpendicular to the conveyor belt's conveying direction. And / or, the two ends of the liquid collection tank extend beyond the sides of the conveyor belt; And / or, the second spraying device is connected via a pipe to a cleaning fluid storage container located below the liquid collection tank or outside the cleanroom. And / or, the bottom of the liquid collection tank is connected via a pipe to a waste liquid container located below it or outside the cleanroom; And / or, the second spraying device sprays liquid upwards and toward the proximal end of the conveyor belt. And / or, the drying device includes an electric heating wire and a first fan; And / or, the cooling device includes a water-cooled pipe and a second fan, the water-cooled pipe being connected to a cold water circulation system outside the cleanroom.

9. The liquid biological product virus inactivation device according to claim 1, characterized in that, The conveyor belt includes a frame, a drive unit, a transmission unit, and a track. The frame includes a horizontal support plate and a support frame, with the upper end of the support frame fixedly connected to the horizontal support plate. The drive unit includes a motor mounted on the horizontal support plate. The transmission device includes a drive roller disposed at one end of the horizontal support plate and a driven roller disposed at the other end of the horizontal support plate. The drive roller is connected to the motor, and the motor can drive the drive roller to rotate. The track is fitted onto the drive roller, the horizontal support plate, and the driven roller.

10. The liquid biological product virus inactivation device according to claim 1, characterized in that, The liquid biological product virus inactivation device also includes a control console and a power module disposed outside the cleanroom. The power module is used to supply power to the equipment inside the cleanroom. The control console is electrically connected to the equipment inside the cleanroom and is used to control the operation of the equipment inside the cleanroom. And / or, the cleanroom is provided with a viewing window; And / or, the cleanroom is equipped with a temperature sensor, a hygrometer, and an ultraviolet radiometer.

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