Portable constant temperature amplification and fluorescence detection integrated detection device

Abstract

This utility model discloses a portable integrated isothermal amplification and fluorescence detection device, relating to the field of nucleic acid detection technology, including a detector, a touch screen, and a storage cavity. The portable integrated isothermal amplification and fluorescence detection device disclosed in this utility model allows the cartridge to slide along a rail into the storage cavity, thereby allowing the reaction tubes inside the test tube holder to enter the detector for monitoring. This enables batch testing of samples, eliminating the need for frequent replacement of the reaction tubes to be tested within the storage cavity by the experimenter, thus improving detection efficiency. Furthermore, after removing the cartridge, simply lowering the cartridge allows the test tube holder to be removed, facilitating the removal of the tested reaction tubes. Moreover, the experimenter only needs to replace the test tube holder and a spare test tube holder, and then move the cartridge back into the detector to perform batch testing again, further improving detection efficiency.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid detection technology, specifically a portable integrated detection device for isothermal amplification and fluorescence detection. Background Technology

[0002] Isothermal amplification is a technique that rapidly amplifies nucleic acids at a constant temperature using specialized enzymes and primers. Fluorescence detection is a technique that monitors and determines the success of the isothermal amplification reaction in real time by detecting changes in the intensity of the fluorescence signal, transforming the invisible nucleic acid amplification process into a visible and measurable change in light signal. A portable integrated isothermal amplification and fluorescence detection device is a miniaturized, portable molecular diagnostic device that integrates isothermal nucleic acid amplification and real-time fluorescence detection functions.

[0003] When staff conduct nucleic acid testing on samples, they need to first mix the processed samples with isothermal amplification fluorescent detection reagents, dispense them into reaction tubes, and then place the reaction tubes into the detection instrument for monitoring. However, this detection method can only monitor an independent reaction tube per batch, making it difficult to test multiple reaction tubes simultaneously, which leads to longer testing time and affects the detection efficiency of the equipment. Utility Model Content

[0004] The purpose of this invention is to provide a portable integrated isothermal amplification and fluorescence detection device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a portable isothermal amplification and fluorescence detection integrated detection device, including a detector, a touch screen display, and a storage cavity. The touch screen display is embedded in the center of the top of the detector, and a storage cavity is opened on one side inside the detector. A card box is connected inside the storage cavity, and a test tube holder is slidably connected inside the card box. The test tube holder has equally spaced storage slots inside, and multiple sets of test tubes can be placed inside the storage slots. Then, the test tube holder is moved into the detector by the card box for batch detection. The card box has a movable groove in the middle of its bottom end, and a base is connected inside the movable groove. The base has a "T" shaped structure, and a base is fixedly connected to the top of the base. The base is located at the bottom of the inner wall of the card box, and the base can move upward to push the test tube holder out of the card box.

[0006] By pressing down on the card holder, the base will be fixed in place against the work surface. The card holder will move the test tube holder downwards, and the base at the top of the base will exert a pushing force on the test tube holder, causing the top of the test tube holder to move out of the test tube holder.

[0007] Preferably, the base and the pedestal are in the shape of an "I" shape, the card box has sliding grooves on both sides of the middle part, and the sliding grooves are connected to sliding rails, and one side of the outer wall of the sliding rails is fixedly connected to the detector.

[0008] The experimenters inserted the reaction tubes into the test tube holder one by one. At this time, the test tube holder was located inside the card box. Then, the card box was moved along the slide rail into the storage cavity. At this time, the slide rail supported and limited the card box to prevent the card box from moving the test tube holder downward.

[0009] Preferably, a sealing plate is fixedly connected to one side of the outer wall of the card box, and a handle is installed in the middle of the outer wall of the sealing plate, the handle being in the shape of a "C".

[0010] After the detector finishes testing the reaction tubes of this batch inside, the experimenter grabs the handle and moves the sealing plate. The movement of the sealing plate then moves the card box along with it until the card box is removed from the storage cavity.

[0011] Preferably, the sealing plate is connected to the upper and lower sides with fixing blocks, and a magnetic strip is magnetically connected to the middle of the outer wall of the fixing blocks, and one side of the outer wall of the magnetic strip is fixedly connected to the detector.

[0012] When the sealing plate is attached to the outer wall of the detector, the fixing blocks on the upper and lower sides of the sealing plate are magnetically connected to the magnetic strip, thereby limiting and fixing the sealing plate to prevent it from moving at will.

[0013] Preferably, a storage box is installed on one side of the outer wall of the detector, and the storage box has storage cavities on the upper and lower sides, and a spare test tube holder is connected inside the storage cavity.

[0014] When the test tube holder is damaged, the laboratory personnel can quickly remove the spare test tube holder to continue the monitoring work.

[0015] Preferably, the spare test tube holder has equally spaced test tube holes inside, and the bottom corner of the detector is connected to a support foot.

[0016] When the test tube holder is inside the detector for monitoring, the experimenter can place the untested reaction tubes into the spare test tube holder for prior preparation.

[0017] As can be seen from the above, the portable isothermal amplification and fluorescence detection integrated detection device provided by this utility model has the following beneficial effects.

[0018] 1. By allowing the card box to slide along the rail into the storage cavity, the reaction tube inside the test tube holder can be brought into the detector for monitoring, thus enabling batch testing of samples. This eliminates the need for frequent replacement of the reaction tubes to be tested in the storage cavity by the experimenter, improving testing efficiency. Furthermore, after removing the card box, the test tube holder can be removed simply by lowering the card box, making it convenient for the experimenter to remove the completed reaction tubes.

[0019] 2. When the test tube holder is damaged, the experimenter can quickly take out the spare test tube holder to continue the monitoring work. The experimenter can also put the untested reaction tubes into the spare test tube holder for preparation. After the reaction tubes in the test tube holder have finished monitoring and are removed from the detector, the experimenter only needs to replace the test tube holder and the spare test tube holder, and then move the card box into the detector to perform batch testing again, thereby further improving the testing efficiency. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the present invention; Figure 2 This is a side view of the three-dimensional structure of the present invention; Figure 3 This is a side view of the structure of this utility model; Figure 4 This is a three-dimensional structural diagram of the sealing plate of this utility model; Figure 5 This is a three-dimensional structural diagram of the card box of this utility model; Figure 6 This is a side view sectional diagram of the card holder structure of this utility model; Figure 7 This is a three-dimensional structural diagram of the base of this utility model; Figure 8 This is a three-dimensional cross-sectional view of the storage box of this utility model.

[0021] In the diagram: 1. Detector; 2. Touch screen; 3. Storage cavity; 4. Card box; 5. Test tube holder; 6. Storage slot; 7. Base; 8. Base; 9. Slide rail; 10. Sealing plate; 11. Handle; 12. Fixing block; 13. Magnetic strip; 14. Storage box; 15. Spare test tube holder. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] Please see Figures 1-8This utility model provides a technical solution: a portable isothermal amplification and fluorescence detection integrated detection device, including a detector 1, a touch screen 2, and a storage cavity 3. The touch screen 2 is embedded in the middle of the top of the detector 1, and a storage cavity 3 is opened on one side inside the detector 1. A card box 4 is connected inside the storage cavity 3, and a test tube holder 5 is slidably connected inside the card box 4. The test tube holder 5 has equally spaced storage slots 6 inside, and multiple sets of test tubes can be placed inside the storage slots 6. Then, the test tube holder 5 is moved into the detector 1 by the card box 4 for batch detection. The card box 4 has a movable groove in the middle of its bottom end, and a base 7 is connected inside the movable groove. The base 7 has a "T" shaped structure, and a base 8 is fixedly connected to the top of the base 7. The base 8 is located at the bottom of the inner wall of the card box 4, and the base 8 can move upward to push the test tube holder 5 out of the card box 4. The base 7 and the base 8 have an "I" shaped structure. Sliding grooves are opened on both sides of the middle of the card box 4, and sliding rails 9 are connected inside the sliding grooves. One side of the outer wall of the sliding rail 9 is fixedly connected to the detector 1.

[0024] In practice, the experimenters first collect samples and extract nucleic acids. Then, they mix the processed samples with isothermal amplification fluorescence detection reagents and dispense them into reaction tubes. At this time, multiple sets of reaction tubes are placed on one side of the workbench. The experimenters then insert the reaction tubes into the test tube holder 5 one by one. At this time, the test tube holder 5 is located inside the cartridge 4. The cartridge 4 is then moved along the slide rail 9 into the storage cavity 3. The slide rail 9 supports and limits the cartridge 4 to prevent the cartridge 4 from moving the test tube holder 5 downward. This allows the reaction tubes inside the test tube holder 5 to enter the detector 1 for monitoring. Then, by detecting the change in the intensity of the fluorescence signal, the experimenters monitor and judge in real time whether the isothermal amplification reaction is successful. The detection results are then displayed on the touch screen 2, thus enabling batch detection of samples without the need for the experimenters to frequently change the reaction tubes to be tested in the storage cavity 3, thereby improving detection efficiency.

[0025] See Figures 5-7 After the detector 1 completes the testing of the batch of reaction tubes inside, the experimenter grasps the handle 11 and moves the sealing plate 10. Then, the movement of the sealing plate 10 will move the card box 4 together until the card box 4 is moved out of the storage cavity 3. At this time, the card box 4 is disengaged from the slide rail 9. Then, the experimenter moves the card box 4 so that the base 7 is located at the edge of the workbench. Then, the experimenter presses down on the card box 4. At this time, the base 7 is attached to the workbench and fixed. The card box 4 moves the test tube holder 5 down. The base 8 at the top of the base 7 will push the test tube holder 5, causing the top of the test tube holder 5 to move out of the test tube holder 5. After removing the card box 4, the test tube holder 5 can be removed simply by moving the card box 4 down, which makes it convenient for the experimenter to remove the tested reaction tubes.

[0026] See Figures 1-4 , Figure 8A sealing plate 10 is fixedly connected to one side of the outer wall of the card box 4, and a handle 11 is installed in the middle of the outer wall of the sealing plate 10. The handle 11 has a "C" shaped structure. Fixing blocks 12 are connected to the upper and lower sides of the sealing plate 10, and a magnetic strip 13 is magnetically connected to the middle of the outer wall of the fixing block 12. One side of the outer wall of the magnetic strip 13 is fixedly connected to the detector 1. A storage box 14 is installed on one side of the outer wall of the detector 1, and storage cavities are opened on the upper and lower sides inside the storage box 14. Spare test tube holders 15 are connected inside the storage cavities. The spare test tube holders 15 have equally spaced test tube holes inside, and support feet are connected to the bottom corners of the detector 1.

[0027] In practice, since the storage box 14 contains a spare test tube holder 15, when the test tube holder 5 is damaged, the experimenter can quickly take out the spare test tube holder 15 to continue the monitoring work. Moreover, the experimenter can take out the test tube holder 5 and the spare test tube holder 15 at the same time in advance. Then, when the test tube holder 5 is inside the detector 1 for monitoring, the experimenter can put the untested reaction tubes into the spare test tube holder 15 for preparation. After the reaction tubes inside the test tube holder 5 have finished monitoring and are removed from the detector 1, the experimenter only needs to replace the test tube holder 5 and the spare test tube holder 15, and then move the card box 4 into the detector 1 to perform batch testing again, thereby further improving the testing efficiency.

[0028] See Figures 1-4 When the sealing plate 10 is attached to the outer wall of the detector 1, the fixing blocks 12 on the upper and lower sides of the sealing plate 10 are attached to the magnetic strip 13 in a magnetic attraction connection, thereby limiting and fixing the sealing plate 10 to prevent it from moving at will.

[0029] The above description is merely a preferred embodiment of this utility model, but the protection scope of this utility model is not limited thereto. The substitutions may be replacements of some structures, devices, or method steps, or they may be complete technical solutions. Equivalent substitutions or modifications made based on the technical solution and inventive concept of this utility model should all be covered within the protection scope of this utility model.

Claims

1. A portable isothermal amplification and fluorescence detection integrated detection device, comprising a detector (1), a touch screen (2), and a storage cavity (3), wherein the touch screen (2) is embedded in the middle of the top of the detector (1), and the storage cavity (3) is provided on one side inside the detector (1), characterized in that: The storage cavity (3) is connected to a card box (4), and a test tube holder (5) is slidably connected inside the card box (4). The test tube holder (5) has equally spaced storage slots (6) inside. Multiple sets of test tubes can be placed inside the storage slots (6). Then, the test tube holder (5) is moved into the detector (1) for batch testing as the card box (4) drives it. The card box (4) has a movable groove in the middle of the bottom end, and a base (7) is connected inside the movable groove. The base (7) has a "T" shaped structure, and a base (8) is fixedly connected to the top of the base (7). The base (8) is located at the bottom of the inner wall of the card box (4), and the base (8) can move upward to push the test tube fixing seat (5) out of the card box (4). 2.The portable integrated device for constant temperature amplification and fluorescence detection according to claim 1, characterized in that: The base (7) and the base (8) are in the shape of an "I". The card box (4) has a sliding groove on both sides in the middle, and a sliding rail (9) is connected inside the sliding groove. The outer wall of the sliding rail (9) is fixedly connected to the detector (1). 3.The portable integrated device for constant temperature amplification and fluorescence detection according to claim 2, characterized in that: A sealing plate (10) is fixedly connected to one side of the outer wall of the card box (4), and a handle (11) is installed in the middle of the outer wall of the sealing plate (10). The handle (11) has a "C" shaped structure. 4.The portable integrated device for constant temperature amplification and fluorescence detection according to claim 3, characterized in that: The sealing plate (10) is connected to the upper and lower sides by fixing blocks (12), and the middle of the outer wall of the fixing block (12) is magnetically connected to a magnetic strip (13). One side of the outer wall of the magnetic strip (13) is fixedly connected to the detector (1).

5. The portable integrated device for constant temperature amplification and fluorescence detection according to claim 4, characterized in that: The detector (1) has a storage box (14) installed on one side of its outer wall, and storage cavities are opened on the upper and lower sides inside the storage box (14), and a spare test tube holder (15) is connected inside the storage cavity. 6.The portable integrated device for constant temperature amplification and fluorescence detection according to claim 5, characterized in that: The spare test tube holder (15) has equally spaced test tube holes inside, and the bottom corner of the detector (1) is connected to a support foot.

Images