A dual-channel optical detection device based on fluorescence detection

Abstract

The utility model discloses a kind of double-channel optical detection devices based on fluorescence detection, it includes metal shielding shell, detection chamber and detection mechanism, detection chamber includes detection bottom plate, transmission light channel is opened in the inside of detection bottom plate, lower cover lens fixed groove is opened in the inside of detection bottom plate and located transmission light channel both sides, detection top plate is opened with upper cover lens fixed groove in the position corresponding lower cover lens fixed groove, optical lens is equipped in lower cover lens fixed groove, lower cover lightproof groove is opened in the top end edge position of detection bottom plate, positioning installation is carried out to optical element by the installation groove inside detection bottom plate and detection top plate mutual cooperation, simple structure is convenient according to the replacement installation of the optical element in inside to carry out detection to different fluorescent dye, make device modularization degree of integration high, make the occupied space inside equipment reduce, reduce the volume of equipment, convenient to carry, improve practicality.

Description

Technical Field

[0001] This utility model relates to the molecular diagnostics industry and the field of fluorescence detectors, and more specifically to a dual-channel optical detection device based on fluorescence detection. Background Technology

[0002] Molecular diagnostics refers to the technique of making a diagnosis by using molecular biology methods to detect changes in the structure or expression level of genetic material in a patient's body.

[0003] Fluorescence is the phenomenon where a substance absorbs light energy and enters an excited state after being irradiated by incident light of a certain wavelength (usually ultraviolet or X-rays), and is immediately excited to emit outgoing light with a wavelength longer than the incident light. Fluorescent dyes are substances that can emit light with a wavelength longer than the absorbed light after absorbing a certain wavelength of light.

[0004] Molecular diagnostic reagents typically use fluorescent dyes as markers. Changes in the fluorescent dyes indicate changes in substances during molecular diagnostics, especially in PCR reactions.

[0005] PCR (polymerase chain reaction) is a molecular biology technique for detecting and amplifying specific nucleic acid fragment sequences in vitro. Due to its high specificity, high sensitivity, and speed, it is widely used in molecular biology detection and analysis. Currently used PCR instruments (nucleic acid amplification detectors) mainly use high-definition camera imaging mode or PMT detection modules for signal acquisition. There are also multi-channel single-channel integrated detection devices for signal detection. They are generally large in size and consume a lot of power, which is not conducive to the use of small rapid diagnostic instruments.

[0006] Insufficient technology: Existing fluorescence acquisition devices typically use dynamic lens combinations to achieve fluorescence acquisition and detection, requiring a supporting mechanical motion structure. This structure is complex and bulky, failing to meet the requirements of small-volume structures. When the structure is installed in a moving carrier, the stability of the optical path structure cannot be guaranteed. Existing fluorescence structures and detection instruments are highly coupled, making independent use impossible and preventing simultaneous detection of multiple instruments as needed, significantly reducing efficiency. During operation, the internal electronic components of the fluorescence acquisition device are highly susceptible to external electromagnetic fields. However, existing equipment cannot effectively isolate external electromagnetic fields, causing detection deviations and greatly reducing the accuracy of the detection results. Utility Model Content

[0007] In order to overcome the above-mentioned defects of the prior art, the present invention provides a dual-channel optical detection device based on fluorescence detection to solve the problems existing in the background art.

[0008] This utility model provides the following technical solution: a dual-channel optical detection device based on fluorescence detection, comprising a metal shielding shell, and further comprising: Testing chamber: installed inside the metal shielding shell and fixedly connected to the metal shielding shell; Testing facility: installed inside the testing room and fixedly connected to the testing room.

[0009] Furthermore, the metal shielding shell includes a lower shell, a second threaded hole on the side of the lower shell, an upper shell at the top of the lower shell, a first threaded hole at the top of the upper shell, a circuit board fixedly connected to the bottom of the inner part of the lower shell, a first fixing bolt threadedly connected to the second threaded hole, a second fixing bolt threadedly connected to the first threaded hole, and a fixing block fixedly connected to the side of the upper shell near the lower shell. Both the upper shell and the lower shell are made of aluminum alloy.

[0010] Furthermore, the testing chamber includes a testing base plate, the side of which is connected to the lower housing via a first fixing bolt. A light transmission channel is formed inside the testing base plate. A first lower cover light source mounting slot and a second lower cover light source mounting slot are formed inside the testing base plate near the light transmission channel. A first lower cover receiving light source mounting slot and a second lower cover receiving light source mounting slot are also formed inside the testing base plate near the light transmission channel. A first lower cover lens fixing slot, a second lower cover lens fixing slot, a third lower cover lens fixing slot, a fourth lower cover lens fixing slot, a fifth lower cover lens fixing slot, a sixth lower cover lens fixing slot, and a seventh lower cover lens fixing slot are formed on both sides of the light transmission channel. An optical lens lower cover mounting slot is formed on one side of the light transmission channel. A lower cover threaded hole is formed at the top of the testing base plate. A testing top plate is formed at the top of the testing base plate, and the bottom end of the testing top plate corresponds to the first lower cover light source mounting slot and the second lower cover. The light source mounting slot has a first upper cover light source mounting slot and a second upper cover light source mounting slot. The bottom end of the detection top plate has a first upper cover light source mounting slot and a second upper cover light source mounting slot corresponding to the positions of the first lower cover light source mounting slot and the second lower cover light source mounting slot. The bottom end of the detection top plate has an optical lens upper cover mounting slot corresponding to the position of the optical lens lower cover mounting slot. The bottom end of the detection top plate has a first upper cover lens fixing slot, a second upper cover lens fixing slot, a third upper cover lens fixing slot, a fourth lower cover lens fixing slot, a fifth lower cover lens fixing slot, a sixth lower cover lens fixing slot, and a seventh lower cover lens fixing slot corresponding to the positions of the first lower cover lens fixing slot, the second lower cover lens fixing slot, the third upper cover lens fixing slot, the fourth upper cover lens fixing slot, the fifth upper cover lens fixing slot, the sixth upper cover lens fixing slot, and the seventh upper cover lens fixing slot. The top end of the detection top plate has an upper cover threaded hole corresponding to the position of the lower cover threaded hole.

[0011] Furthermore, the detection mechanism includes a first laser emitting device, which is located in a first lower cover light source mounting slot. A first light source receiving device is located in a first lower cover light source receiving mounting slot. A second laser emitting device is located in a second lower cover light source mounting slot. A second light source receiving device is located in a second lower cover light source receiving mounting slot. An optical lens device is located in an optical lens lower cover mounting slot. A first optical lens is located in a first lower cover lens fixing slot. A second optical lens is located in a second lower cover lens fixing slot. A third optical lens is located in a third lower cover lens fixing slot. A seventh optical lens is located in a fourth lower cover lens fixing slot. A fifth optical lens is located in a fifth lower cover lens fixing slot. A sixth optical lens is located in a sixth lower cover lens fixing slot. A fourth optical lens is located in a seventh lower cover lens fixing slot.

[0012] Furthermore, a lower cover light-dense groove is provided at the top edge of the detection base plate, and an upper cover light-dense groove is provided at the bottom of the detection top plate corresponding to the lower cover light-dense groove. The lower cover light-dense groove and the upper cover light-dense groove can be closed to each other to keep the light blocked. The detection top plate is connected and fixed by a second fixing bolt, and the upper shell, lower shell and detection base plate are fixedly connected by a first fixing bolt.

[0013] Furthermore, mounting grooves are provided at the edges of the first, second, third, fourth, fifth, sixth, and seventh optical lenses.

[0014] Furthermore, the inner walls of both the lower and upper shells are provided with elastic cushioning material.

[0015] The technical effects and advantages of this utility model are as follows: This invention features a lower housing made of aluminum alloy to protect the internal optical components, which helps to isolate the device from external electromagnetic fields, extend the service life of the equipment, and ensure the stability of optical signal acquisition.

[0016] This utility model features a lower and upper cover for the detection chamber, with mounting slots inside for installing and fixing optical components. This reduces the use of fixed parts and facilitates easy replacement of internal optical components for fluorescence detection as needed. The high degree of modular integration reduces the internal space occupied by the equipment, making it smaller, easier to carry, and improving its practicality.

[0017] This invention uses optical elements inside the detection mechanism to form two sets of fluorescence detection mechanisms, which can be combined with corresponding optical lenses to detect two fluorescent dyes simultaneously, thus improving the efficiency of the detection work.

[0018] This invention connects to the outside world and communicates with it via a circuit board and ribbon cable, enabling the independent use of the module and improving the diversity of application scenarios for the detection module. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0020] Figure 2 This is a schematic diagram showing the overall structure of this utility model.

[0021] Figure 3 This is a schematic diagram of the detection element structure of this utility model.

[0022] Figure 4 This is a schematic diagram of the mounting groove for the detection base plate of this utility model.

[0023] Figure 5 This is a schematic diagram of the mounting groove for the detection top plate of this utility model.

[0024] Figure 6 This is a schematic diagram of the optical structure of the detection element of this utility model.

[0025] Figure 7 This is a schematic diagram of the optical path structure operating principle of this utility model.

[0026] Figure 8 This is a schematic diagram of the optical path element of this utility model.

[0027] The attached figures are labeled as follows: 1. Metal shielding shell; 101. Lower shell; 102. Upper shell; 103. First fixing bolt; 104. First threaded hole; 105. Second threaded hole; 106. Fixing block; 107. Second fixing bolt; 2. Detection chamber; 201. Detection base plate; 202. Detection top plate; 203. Upper cover light-dense groove; 204. First lower cover light-receiving light-mounting groove; 205. First lower cover light-mounting groove; 206. Lower cover light-dense groove; 207. First lower cover mirror. 208. Second lower cover lens fixing slot; 209. Second lower cover light source mounting slot; 210. Second lower cover light source receiving mounting slot; 211. Third lower cover lens fixing slot; 212. Fourth lower cover lens fixing slot; 213. Fifth lower cover lens fixing slot; 214. Sixth lower cover lens fixing slot; 215. Seventh lower cover lens fixing slot; 216. Optical lens lower cover mounting slot; 217. Lower cover threaded hole; 218. Upper cover threaded hole; 219. First upper cover light receiving... 220. Source mounting slot; 221. First upper cover receiving light source mounting slot; 222. First upper cover lens fixing slot; 223. Second upper cover lens fixing slot; 224. Third upper cover lens fixing slot; 225. Second upper cover receiving light source mounting slot; 226. Second upper cover light source mounting slot; 227. Fifth upper cover lens fixing slot; 228. Sixth upper cover lens fixing slot; 229. Seventh upper cover lens fixing slot; 230. Optical lens upper cover mounting slot; 231. Transmission light channel; 3. Detection mechanism; 301. First laser emitting device; 302. First light source receiving device; 303. Second laser emitting device; 304. Second light source receiving device; 305. First optical lens; 306. Second optical lens; 307. Third optical lens; 308. Fourth optical lens; 309. Fifth optical lens; 310. Sixth optical lens; 311. Optical lens assembly; 312. Seventh optical lens; 4. Circuit board. Detailed Implementation

[0028] The technical solution of this utility model will be clearly and completely described below with reference to the accompanying drawings. In addition, the forms of the various structures described in the following embodiments are merely illustrative. The fluorescence collection device involved in this utility model is not limited to the structures described in the following embodiments. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0029] Reference Figure 1-5 This utility model provides a dual-channel optical detection device based on fluorescence detection, including a metal shielding shell 1, and further comprising: Testing chamber 2: installed inside the metal shielding shell 1 and fixedly connected to the metal shielding shell 1; Testing unit 3: installed inside testing chamber 2 and fixedly connected to testing chamber 2.

[0030] The metal shielding shell 1 includes a lower shell 101, a second threaded hole 105 on the side of the lower shell 101, an upper shell 102 at the top of the lower shell 101, a first threaded hole 104 at the top of the upper shell 102, a circuit board 4 fixedly connected to the bottom of the interior of the lower shell 101, a first fixing bolt 103 threadedly connected to the second threaded hole 105, a second fixing bolt 107 threadedly connected to the first threaded hole 104, and a fixing block 106 fixedly connected to the side of the upper shell 102 near the first fixing bolt 103. Both the upper shell 102 and the lower shell 101 are made of aluminum alloy. By using aluminum alloy, the shell is not only sturdy and durable but also has a good shielding effect against electromagnetic interference, reducing external signal interference and reducing the interference of external factors on the final data acquisition results.

[0031] The testing chamber 2 includes a testing base plate 201. The side of the testing base plate 201 is connected to the lower housing 101 by a first fixing bolt 103. A transmission light channel 231 is formed inside the testing base plate 201. A first lower cover light source mounting groove 205 and a second lower cover light source mounting groove 209 are formed inside the testing base plate 201 near the transmission light channel 231. A first lower cover receiving light source mounting groove 204 and a second lower cover receiving light source mounting groove 210 are formed inside the testing base plate 201 near the transmission light channel 231. The transmission light channel 231 has a first lower cover receiving light source mounting groove 204 and a second lower cover receiving light source mounting groove 210 on both sides. The optical transmission channel 231 has a lower lens mounting slot 216 on one side inside, and a lower lens mounting slot 216 on one side inside. The top of the detection base plate 201 has a lower lens threaded hole 217, and the top of the detection base plate 201 has a detection top plate 202. The bottom of the detection top plate 202 corresponds to the first lower lens mounting slot 205 and the second lower lens mounting slot. Position 209 provides a first upper cover light source mounting slot 219 and a second upper cover light source mounting slot 226. The bottom end of the detection top plate 202, corresponding to the positions of the first lower cover light source mounting slot 204 and the second lower cover light source mounting slot 210, provides a first upper cover light source mounting slot 220 and a second upper cover light source mounting slot 225. The bottom end of the detection top plate 202, corresponding to the position of the optical lens lower cover mounting slot 216, provides an optical lens upper cover mounting slot 230. The bottom end of the detection top plate 202, corresponding to the positions of the first lower cover lens fixing slot 207, the second lower cover lens fixing slot 208, and the... The positions of the lower cover lens fixing groove 211, the fourth lower cover lens fixing groove 212, the fifth lower cover lens fixing groove 213, the sixth lower cover lens fixing groove 214, and the seventh lower cover lens fixing groove 215 are provided with the first upper cover lens fixing groove 221, the second upper cover lens fixing groove 222, the third upper cover lens fixing groove 223, the fourth upper cover lens fixing groove 224, the fifth upper cover lens fixing groove 227, the sixth upper cover lens fixing groove 228, and the seventh upper cover lens fixing groove 229. The top of the detection top plate 202 is provided with the upper cover threaded hole 218 corresponding to the position of the lower cover threaded hole 217.

[0032] The testing mechanism 3 includes a first laser emitting device 301, which is located in the first lower cover light source mounting slot 205. A first light source receiving device 302 is located in the first lower cover light source receiving mounting slot 204. A second laser emitting device 303 is located in the second lower cover light source mounting slot 209. A second light source receiving device 304 is located in the second lower cover light source receiving mounting slot 210. An optical lens device 311 is located in the optical lens lower cover mounting slot 216. A first optical lens 305 is located in the first lower cover lens fixing slot 207. A second optical lens 306 is located in the second lower cover lens fixing slot 208. A third optical lens 307 is located in the third lower cover lens fixing slot 211. A seventh optical lens 312 is located in the fourth lower cover lens fixing slot 212. A fifth optical lens 309 is located in the fifth lower cover lens fixing slot 213. A sixth optical lens 310 is located in the sixth lower cover lens fixing slot 214. A fourth optical lens 308 is located in the seventh lower cover lens fixing slot 215.

[0033] The bottom edge of the detection base plate 201 is provided with a lower cover light-dense groove 206, and the bottom end of the detection top plate 202 is provided with an upper cover light-dense groove 203 corresponding to the lower cover light-dense groove 206. The lower cover light-dense groove 206 and the upper cover light-dense groove 203 can be closed to each other to keep the light blocked. The detection top plate 202 is connected and fixed by a second fixing bolt 107. The upper shell 102, the lower shell 101 and the detection base plate 201 are fixedly connected by a first fixing bolt 103. By using bolts to fix them, the device will be more robust during transportation and will not be damaged by external forces.

[0034] Among them, the first optical lens 305, the second optical lens 306, the third optical lens 307, the fourth optical lens 308, the fifth optical lens 309, the sixth optical lens 310 and the seventh optical lens 312 are all provided with mounting grooves at their edges. By providing elastic anti-slip pads at the edges of each optical lens, the optical lenses are fixed to the edges of the optical lenses, thus protecting them and preventing damage.

[0035] The inner walls of both the lower housing 101 and the upper housing 102 are provided with elastic buffer material to protect the inside of the equipment and prevent damage to the internal optical components caused by impacts.

[0036] The working principle of this utility model is as follows: First, the device is inspected before use. A suitable optical lens is selected based on the fluorescent dye to be detected, and the optical lens is installed in the corresponding mounting slot. The device is then fixed using the first fixing bolt 103 and the second fixing bolt 107. The installed optical lens device 311 is aligned with the fluorescent dye to be detected. Simultaneously, the circuit board 4 drives the device. The device can be controlled and the detection data can be viewed via an external connection cable. The detection mechanism 3 contains a fluorescence detection mechanism composed of two sets of optical elements, which, through the combination of corresponding optical lenses, can simultaneously detect two different fluorescent dyes. When a second-path fluorescence detection is required, the second laser emitting device 303 emits a detection emission beam. This beam passes through the fourth optical lens 308 and is reflected onto the optical lens device 311. The optical lens device 311 focuses the beam onto the fluorescence object being detected. The emitted fluorescence beam travels back along the optical path through the optical lens device 311, through the fourth optical lens 308, and reaches the fifth optical lens 309. The fifth optical lens 309 reflects the light back to the second light source receiving device 304, where fluorescence data is collected. When the second laser emitting device 303 is operating, the first-path fluorescence laser emitting device 301 also emits a detection beam. The light is irradiated by the third optical lens 307, which reflects the light onto the optical lens device 311. The optical lens device 311 focuses the light onto the fluorescent object to be detected. The light emitted by the fluorescence is returned through the optical lens device 311 along the optical path channel 231, and then through the fourth optical lens 308, the fifth optical lens 309, and the third optical lens 307 to reach the first optical lens 305. The first optical lens 305 reflects the light to the first light source receiving device 302, where fluorescence data is collected. The collected detection data is then transmitted to an external device via the circuit board 4 for viewing. This allows for the simultaneous detection of multiple fluorescent dyes. Since the product is controlled and operates by circuit board 4, the stored data can be viewed. The casing is made of a special aluminum alloy, which not only protects the internal components but also isolates the device from external electromagnetic fields, preventing interference with normal operation, thus improving stability and extending lifespan. Furthermore, improvements to the internal components allow for the replacement of different optical elements, significantly enhancing performance and enabling the detection and processing of various fluorescent dyes. The highly integrated internal modules also result in a small internal footprint, making it easy to carry and greatly improving portability.

[0037] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A dual channel optical detection device based on fluorescence detection comprising a metal shielded housing (1), characterized in that, Also includes: Testing chamber (2): installed inside the metal shielding shell (1) and fixedly connected to the metal shielding shell (1); Testing mechanism (3): installed inside the testing chamber (2) and fixedly connected to the testing chamber (2); The testing chamber (2) includes a testing base plate (201). The side of the testing base plate (201) is connected to the lower housing (101) by a first fixing bolt (103). A transmission light channel (231) is provided inside the testing base plate (201). A first lower cover light source mounting groove (205) and a second lower cover light source mounting groove (209) are provided inside the testing base plate (201) near the transmission light channel (231). A first lower cover receiving light source mounting groove (204) and a second lower cover receiving light source mounting groove (210) are provided inside the testing base plate (201) near the transmission light channel (231). The transmission light channel (231) has openings on both sides. The first lower cover lens fixing groove (207), the second lower cover lens fixing groove (208), the third lower cover lens fixing groove (211), the fourth lower cover lens fixing groove (212), the fifth lower cover lens fixing groove (213), the sixth lower cover lens fixing groove (214), and the seventh lower cover lens fixing groove (215) are provided. An optical lens lower cover mounting groove (216) is provided on one side of the transmission light channel (231). A lower cover threaded hole (217) is provided at the top of the detection base plate (201). A detection top plate (202) is provided at the top of the detection base plate (201). The bottom end of the detection top plate (202) corresponds to the first lower cover light source mounting groove (205) and the second lower cover light source mounting groove. The slot (209) is provided with a first upper cover light source mounting slot (219) and a second upper cover light source mounting slot (226). The bottom end of the detection top plate (202) is provided with a first upper cover light source mounting slot (220) and a second upper cover light source mounting slot (225) corresponding to the positions of the first lower cover light source mounting slot (204) and the second lower cover light source mounting slot (210). The bottom end of the detection top plate (202) is provided with an optical lens upper cover mounting slot (230) corresponding to the position of the optical lens lower cover mounting slot (216). The bottom end of the detection top plate (202) is provided with a first lower cover lens fixing slot (207), a second lower cover lens fixing slot (208), and a third lower cover lens fixing slot (209). The first upper cover lens fixing groove (221), the second upper cover lens fixing groove (222), the third upper cover lens fixing groove (223), the fourth upper cover lens fixing groove (224), the fifth upper cover lens fixing groove (227), the sixth upper cover lens fixing groove (228), and the seventh upper cover lens fixing groove (229) are provided at the positions of the lower cover lens fixing groove (211), the fourth lower cover lens fixing groove (212), the fifth upper cover lens fixing groove (213), the sixth upper cover lens fixing groove (214), and the seventh upper cover lens fixing groove (229). The top of the detection top plate (202) is provided with an upper cover threaded hole (218) at the position corresponding to the lower cover threaded hole (217).

2. The dual channel fluorescence detection based optical detection device according to claim 1, wherein: The metal shielding shell (1) includes a lower shell (101), a second threaded hole (105) is provided on the side of the lower shell (101), an upper shell (102) is provided at the top of the lower shell (101), a first threaded hole (104) is provided at the top of the upper shell (102), a circuit board (4) is fixedly connected to the bottom of the interior of the lower shell (101), a first fixing bolt (103) is threadedly connected to the second threaded hole (105), a second fixing bolt (107) is threadedly connected to the first threaded hole (104), and a fixing block (106) is fixedly connected to the side of the upper shell (102) near the first fixing bolt (103). Both the upper shell (102) and the lower shell (101) are made of aluminum alloy.

3. The dual channel fluorescence detection based optical detection device according to claim 2, wherein: The detection mechanism (3) includes a first laser emitting device (301), which is located in the first lower cover light source mounting slot (205). A first light source receiving device (302) is located in the first lower cover light source receiving mounting slot (204). A second laser emitting device (303) is located in the second lower cover light source mounting slot (209). A second light source receiving device (304) is located in the second lower cover light source receiving mounting slot (210). An optical lens device (311) is located in the optical lens lower cover mounting slot (216). A first optical lens (305) is located in the first lower cover lens fixing slot (207). A second optical lens (306) is located in the second lower cover lens fixing slot (208). A third optical lens (307) is located in the third lower cover lens fixing slot (211). A seventh optical lens (312) is located in the fourth lower cover lens fixing slot (212). A fifth optical lens (309) is located in the fifth lower cover lens fixing slot (213). The sixth optical lens (310) is provided in the sixth lower cover lens fixing groove (214), and the fourth optical lens (308) is provided in the seventh lower cover lens fixing groove (215).

4. The dual channel optical detection device based on fluorescence detection according to claim 3, characterized in that: The detection base plate (201) has a lower cover light-dense groove (206) at the top edge, and the detection top plate (202) has an upper cover light-dense groove (203) at the bottom corresponding to the lower cover light-dense groove (206). The lower cover light-dense groove (206) and the upper cover light-dense groove (203) can be closed to each other to keep the light out. The detection top plate (202) is connected and fixed by the second fixing bolt (107). The upper shell (102), the lower shell (101) and the detection base plate (201) are fixedly connected by the first fixing bolt (103).

5. The dual channel fluorescence detection based optical detection device according to claim 4, wherein: The first optical lens (305), the second optical lens (306), the third optical lens (307), the fourth optical lens (308), the fifth optical lens (309), the sixth optical lens (310), and the seventh optical lens (312) are all provided with mounting grooves at their edges.

6. The dual channel fluorescence detection based optical detection device according to claim 2, wherein: The inner walls of both the lower shell (101) and the upper shell (102) are provided with elastic cushioning material.

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