A colloidal gold pesticide residue detection device

Abstract

The utility model relates to food safety detection technical field discloses a colloidal gold pesticide residue detection device, including upper half casing, lower half casing and reaction strip, upper half casing and lower half casing are adapted to each other, and are sealed at the connecting place of both, and the reaction strip is detachably installed in the complete casing, and a plurality of rear are evenly arranged along the circumference of complete casing, and the center of upper half casing and lower half casing corresponds and reserves detection hole, and one end of reaction strip extends to detection hole, and upper half casing corresponds and reserves observation port respectively for each reaction strip, compared with prior art, the advantage lies in: the novel can accommodate six reaction strips (test paper strip) simultaneously through the unique hexagonal disc type integrated design, realizes six different pesticide residue indexes to be detected simultaneously in one sample adding, completely changes the cumbersome mode of traditional single connection detection card one by one operation, greatly improves the detection efficiency, and is especially suitable for the occasion that needs to carry out rapid screening to multiple pesticides.

Description

Technical Field

[0001] This utility model relates to the field of food safety testing technology, specifically to a colloidal gold pesticide residue detection device. Background Technology

[0002] Colloidal gold immunochromatography, based on the labeling of gold nanoparticles and the principle of immunochromatography, is characterized by rapid detection and ease of operation. It has been widely applied in fields such as medical diagnostics and food safety, particularly in the detection of pesticide residues in vegetables, veterinary drug residues in livestock and aquatic products, and mycotoxins in grains. The detection card for this technology typically consists of a sample pad, a gold-labeled antibody-binding pad, a nitrocellulose membrane (containing test lines and control lines), and an absorbent pad, completing the detection through capillary action.

[0003] Currently, the detection of pesticide residues in vegetables (such as chlorpyrifos, carbofuran, methamidophos, methomyl, triazophos, fipronil, iprodione, acetamiprid, chlorothalonil, carbendazim, etc.) using colloidal gold immunochromatography requires the use of separate test cards, which is cumbersome and involves steps such as unpacking, placing, and adding liquid, resulting in high workload and low detection efficiency. Utility Model Content

[0004] The technical problem to be solved by this utility model is to overcome the above-mentioned technical difficulties and provide a colloidal gold pesticide residue detection device to solve the problems of cumbersome operation and low efficiency in the prior art.

[0005] To solve the above-mentioned technical problems, the technical solution provided by this utility model is as follows: A colloidal gold pesticide residue detection device includes an upper shell, a lower shell, and reaction strips. The upper and lower shells are adapted to each other and form a complete shell. The connection between the two is sealed. The reaction strips are detachably installed inside the complete shell and are evenly arranged in multiples along the circumference of the complete shell. Detection holes are reserved at the center of the upper and lower shells respectively. One end of the reaction strip extends into the detection hole, and observation ports are reserved on the upper shell for each reaction strip.

[0006] As an improvement, a lower positioning groove is formed on the bottom wall of the lower half of the shell corresponding to each reaction strip, and an upper positioning groove is formed on the top wall of the upper half of the shell corresponding to each reaction strip. The upper and lower positioning grooves are matched and form a complete positioning groove, in which the reaction strip is placed. This facilitates the installation of the reaction strip.

[0007] As an improvement, multiple insertion cylinders are formed on the inner top wall of the upper shell, and insertion sleeves are formed on the inner bottom wall of the lower shell corresponding to each insertion cylinder. After the insertion cylinders are inserted into the insertion sleeves, they are interference-fitted with the insertion sleeves. This makes the assembly of the upper and lower shells more secure.

[0008] As an improvement, finger rings are fixed to the sides of the upper and lower shells respectively, and the two finger rings are staggered vertically. This facilitates the disassembly of the upper and lower shells.

[0009] The advantages of this utility model compared with the prior art are as follows: 1. This new type of device, through its unique hexagonal disc integrated design, can simultaneously accommodate six reaction strips (test strips), enabling the simultaneous detection of six different pesticide residue indicators with a single sample addition. This completely changes the cumbersome operation mode of traditional single-sheet test cards, greatly improving detection efficiency, and is particularly suitable for occasions requiring rapid screening of multiple pesticides.

[0010] 2. With this new method, users no longer need to repeatedly open bags, remove cards, arrange items, and add samples multiple times. The entire testing process can be completed in a single sample addition step. This significantly reduces the labor intensity and technical requirements for operators, while also reducing random errors introduced by multiple operations, thus improving the reliability and consistency of test results.

[0011] 3. This new type of housing adopts a split upper and lower design, achieving a tight connection through the interference fit between the insert cylinder and the insert sleeve, ensuring the overall sealing of the housing. This structure effectively protects the internal reaction strips, preventing them from being exposed to moisture, contamination, or damage during transportation and storage, thus ensuring the stability and validity period of the test card.

[0012] 4. This new design features integrally formed, star-shaped positioning grooves on the inner walls of the upper and lower housings, providing precise and independent accommodating space for each reaction strip and ensuring its fixed position. The observation ports correspond one-to-one with the detection / control line display areas of the reaction strips, with a neat layout, facilitating quick and accurate interpretation of test results for each item. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of this utility model.

[0014] Figure 2 This is a top view of the present invention.

[0015] Figure 3 This is an exploded view of the present invention.

[0016] As shown in the figure: 1. Upper shell; 2. Lower shell; 3. Reaction strip; 4. Detection hole; 5. Observation port; 6. Lower positioning groove; 7. Insert sleeve. Detailed Implementation

[0017] In the description of this utility model, it should be understood that the terms "center," "lateral," "upper," "lower," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more. Additionally, the term "comprising" and any variations thereof are intended to cover non-exclusive inclusion.

[0018] The present invention will now be described in further detail with reference to the accompanying drawings.

[0019] A colloidal gold pesticide residue detection device includes: The upper shell 1 and the lower shell 2 are fitted together to form a complete shell, and their connection is sealed. Both the upper shell 1 and the lower shell 2 are hexagonal in shape. Multiple insertable cylinders are formed on the inner top wall of the upper shell 1, and corresponding insertable sleeves 7 are formed on the inner bottom wall of the lower shell 2. The insertable cylinders are inserted into the insertable sleeves 7 with an interference fit. Finger rings are fixed to the sides of both the upper shell 1 and the lower shell 2, and the two finger rings are staggered vertically. The reaction strip 3 has a lower positioning groove 6 formed on the bottom wall of the lower half shell 2 corresponding to each reaction strip 3, and an upper positioning groove formed on the top wall of the upper half shell 1 corresponding to each reaction strip 3. The upper positioning groove and the lower positioning groove 6 are adapted to each other and form a complete positioning groove. The reaction strip 3 is placed in the complete positioning groove. After six reaction strips 3 are evenly arranged along the circumference of the complete shell, each upper positioning groove and each lower positioning groove 6 are integrally formed and form a *-shaped structure. The center of the upper half shell 1 and the lower half shell 2 are respectively reserved with detection holes 4. One end of the reaction strip 3 extends into the detection hole 4, and each reaction strip 3 is reserved with an observation port 5 on the upper half shell 1.

[0020] In the specific implementation of this embodiment: Sample loading: The user adds the sample liquid to be tested (such as vegetable extract) through the detection hole 4 in the center of the upper shell 1 in one go.

[0021] Sample dispensing and siphoning: The sample liquid first flows into the central chamber formed by the upper and lower shells. Since the sample pad ends of the six reaction strips 3 all extend into this chamber, the sample liquid is synchronously and uniformly siphoned onto the sample pads of the six reaction strips 3 through capillary action.

[0022] Chromatographic reaction: The sample solution continues to chromatographically advance along reaction strip 3, flowing sequentially through: Gold-labeled antibody binding pad: The target pesticide residue in the sample binds to the specific antibody pre-embedded in colloidal gold on the pad to form a complex.

[0023] Nitrocellulose membrane: The complex continues to be chromatographically deposited onto the detection line (T line) and control line (C line) on the membrane.

[0024] The detection line (T line) contains pesticide antigens. If the sample does not contain pesticides or the concentration is below the detection limit, the gold-labeled antibody binds to them, forming a visible red T line. If the pesticide concentration is high, it competitively inhibits the binding of the gold-labeled antibody to the T line, resulting in the T line not developing color or developing a lighter color.

[0025] Control line (C line): Embedded with anti-antibody (such as goat anti-mouse IgG) to capture the remaining gold-labeled antibody. The C line should show color regardless of whether the sample contains pesticides, indicating that the chromatography process was completed normally.

[0026] Result interpretation: After the chromatography reaction is completed (usually 5-10 minutes), the user can directly observe the color development of the T line and C line on each reaction strip 3 through the corresponding observation port 5 on the upper shell 1, and thus determine the residue status of each corresponding pesticide.

[0027] Workflow summary: Single sample addition → Centralized dispensing → Six-channel parallel chromatography → Independent immunoassay → Simultaneous result interpretation. This design cleverly combines fluid dispensing with multi-channel immunoassay, achieving high-throughput, rapid, and convenient detection.

[0028] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the inventive spirit of the present invention, such designs should fall within the protection scope of the present invention.

Claims

1. A colloidal gold pesticide residue detection device, characterized in that: It includes an upper shell (1), a lower shell (2) and a reaction strip (3); the upper shell (1) and the lower shell (2) are adapted to each other and form a complete shell. The connection between the two is sealed. The reaction strip (3) can be detachably installed in the complete shell. After multiple strips are evenly arranged along the circumference of the complete shell, the upper shell (1) and the lower shell (2) are respectively reserved with detection holes (4). One end of the reaction strip (3) extends into the detection hole (4). The upper shell (1) is reserved with observation ports (5) for each reaction strip (3).

2. The colloidal gold pesticide residue detection device according to claim 1, characterized in that: The lower half shell (2) has a lower half positioning groove (6) formed on the bottom wall corresponding to each reaction strip (3), and the upper half shell (1) has an upper half positioning groove formed on the top wall corresponding to each reaction strip (3). The upper half positioning groove and the lower half positioning groove (6) are matched and form a complete positioning groove. The reaction strip (3) is placed in the complete positioning groove.

3. The colloidal gold pesticide residue detection device according to claim 2, characterized in that: The reaction strip (3) is installed in six parts, and each upper half positioning groove and each lower half positioning groove (6) are integrally formed to form a *-shaped structure.

4. The colloidal gold pesticide residue detection device according to claim 3, characterized in that: The upper shell (1) and the lower shell (2) are both hexagonal in shape.

5. The colloidal gold pesticide residue detection device according to claim 1, characterized in that: Multiple insertable cylinders are formed on the inner top wall of the upper shell (1), and insertable sleeves (7) are formed on the inner bottom wall of the lower shell (2) corresponding to each insertable cylinder. After the insertable cylinder is inserted into the insertable sleeve (7), it is interference-fitted with the insertable sleeve (7).

6. The colloidal gold pesticide residue detection device according to claim 1, characterized in that: The upper shell (1) and the lower shell (2) are respectively fixed with finger rings on their sides, and the two finger rings are staggered vertically.

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