Colloidal gold test strips for rapid detection of Seneca virus antibodies

Abstract

This utility model relates to the field of colloidal gold test strip technology, specifically a colloidal gold test strip for rapid detection of Seneca virus antibodies, comprising a bottom shell and an upper shell, which are snapped together to form a box body. The upper surface of the bottom shell has a test strip mounting groove, inside which are respectively arranged an adsorption membrane, a conjugate pad, an NC membrane, and absorbent filter paper. A liquid accumulation groove is located at the bottom of the test strip mounting groove, and a liquid inlet groove is located on the side of the liquid accumulation groove. In use, by setting a liquid accumulation groove at the bottom of the test strip mounting groove corresponding to the dropper opening, and setting a liquid inlet groove on the side of the liquid accumulation groove so that the liquid inlet strip extends into the liquid inlet groove, after the liquid sample is dripped in through the dropper opening, the adsorption membrane adsorbs the sample liquid through capillary action. Part of the sample liquid is transported to the colloidal gold through the adsorption membrane, and part of the sample liquid permeates into the liquid accumulation groove and is directly adsorbed by the liquid inlet strip. By increasing the adsorption area, the detection efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of colloidal gold test strip technology, and in particular to a colloidal gold test strip for rapid detection of Seneca virus antibodies. Background Technology

[0002] Seneca virus is a highly contagious pathogen that primarily affects pigs and belongs to the Picornaviridae family. The disease it causes is called Seneca Valley virus infection, with symptoms including blisters around the mouth and nose, lameness, and anorexia, which are highly similar to foot-and-mouth disease, but the pathogens are different. This virus can be transmitted through direct contact, contaminated objects, or aerosols.

[0003] VP1 protein is a major component of the Seneca virus nucleocapsid protein, and its gene sequence is relatively conserved. Therefore, it is often used as a target protein for Seneca virus diagnosis. Thus, Seneca virus can be detected using colloidal gold test strips.

[0004] Existing colloidal gold test strips mainly consist of: a sample pad that receives the liquid sample and guides it towards the absorbent pad via capillary action; a binding pad pre-coated with colloidal gold-labeled antibodies that bind to the target antigen in the sample to form a complex; and a reaction membrane used to immobilize specific antibodies, capture the target antigen-gold-labeled antibody complex, develop a colorimetric result indicating a positive outcome, and capture unbound gold-labeled antibodies to verify the strip's effectiveness. The entire testing process relies on capillary action to transfer the liquid sample, and after receiving the sample, it often requires 5–10 minutes of stillness to obtain results, resulting in low testing efficiency.

[0005] Therefore, this application proposes a colloidal gold test strip for rapid detection of Seneca virus antibodies to improve detection efficiency. Utility Model Content

[0006] In view of the shortcomings of the prior art described above, the purpose of this utility model is to provide a colloidal gold test strip for rapid detection of Seneca virus antibodies, in order to solve the problem of slow detection speed mentioned in the prior art.

[0007] To achieve the above and other related objectives, this utility model provides a colloidal gold test strip for rapid detection of Seneca virus antibodies, comprising a bottom shell and an upper shell, wherein the bottom shell and the upper shell are snapped together to form a box body; The upper surface of the bottom shell is provided with a test strip mounting groove. The interior of the test strip mounting groove is provided with an adsorption membrane, a conjugation pad, an NC membrane and a water-absorbing filter paper. The bottom of the test strip mounting groove is provided with a liquid accumulation groove, and the side of the liquid accumulation groove is provided with a liquid guiding groove. The water-absorbing filter paper is located at the end of the test strip mounting groove away from the liquid accumulation groove. The upper shell is provided with a drip outlet, which is located directly above the liquid collection tank. The upper shell is provided with a long strip-shaped display window, and the NC membrane is located directly below the display window. The adsorption membrane covers the top of the liquid collection tank, the binding pad abuts against the adsorption membrane, and the bottom of the binding pad extends into the interior of the liquid collection tank. One end of the NC membrane overlaps the binding pad, and the other end of the NC membrane overlaps the absorbent filter paper.

[0008] Preferably, the shape of the drip nozzle is an inverted frustum, and the axis of the frustum is provided with an inverted conical hole.

[0009] Preferably, the adsorption membrane is a thin film prepared from porous cellulose.

[0010] Preferably, the bonding pad comprises colloidal gold, the colloidal gold being Z-shaped, and a liquid-guiding strip is provided at the bottom of the colloidal gold, the liquid-guiding strip extending into the interior of the liquid-guiding groove.

[0011] Preferably, a gap is left between the top of the liquid guide strip and the bottom of the high surface of the colloidal gold, and the end of the adsorption membrane is tightly inserted into this gap.

[0012] Preferably, the liquid inlet groove is a plurality of strip-shaped through grooves, and the liquid inlet strips correspond one-to-one with all the liquid inlet grooves.

[0013] Preferably, the bottom of the test paper mounting groove is provided with a support platform, the bonding pad and the absorbent filter paper are located on both sides of the support platform, and the NC membrane is placed on the top of the support platform.

[0014] Preferably, the NC membrane is provided with two indicator lines, which are a test line and a quality control line, and both the test line and the quality control line are located within the viewing window of the display window.

[0015] Preferably, the top side of the absorbent filter paper facing the NC membrane has a groove, and the end of the NC membrane overlaps the groove.

[0016] As described above, the colloidal gold test strip for rapid detection of Seneca virus antibodies of this invention has the following beneficial effects: 1. This utility model provides a liquid accumulation groove at the bottom of the test strip mounting slot, corresponding to the position of the droplet outlet, and a liquid guide groove on the side of the liquid accumulation groove so that the liquid guide strip extends into the interior of the liquid guide groove. At the same time, the liquid accumulation groove is covered by an adsorption membrane. After the liquid sample is dripped in through the droplet outlet, the adsorption membrane adsorbs the sample liquid through capillary action. Part of the sample liquid is transported to the colloidal gold through the adsorption membrane, and part of the sample liquid permeates into the liquid accumulation groove and is directly adsorbed by the liquid guide strip. Finally, it is transported to the NC membrane through the colloidal gold. By increasing the adsorption area, the detection efficiency is improved.

[0017] 2. This utility model sets the colloidal gold in a Z-shape and forms a gap between the top of the liquid guide strip and the bottom of the high surface of the colloidal gold, allowing the adsorption membrane to be inserted. This allows the sample liquid adsorbed by the adsorption membrane to be transported to the colloidal gold through both the top and bottom surfaces, thereby further improving the detection efficiency.

[0018] 3. This utility model supports the NC membrane by setting a support platform in the test strip mounting groove, thereby reducing the thickness of the NC membrane and allowing the bound liquid to be transported in the NC membrane more quickly. At the same time, it can also reduce the dosage of the test sample.

[0019] Therefore, this utility model effectively overcomes the various shortcomings of the prior art and has high industrial application value. Attached Figure Description

[0020] Figure 1 The diagram shown is a structural schematic of this utility model.

[0021] Figure 2 The diagram shown is a cross-sectional view of the structure of this utility model.

[0022] Figure 3 The diagram shown is a structural assembly diagram of this utility model.

[0023] Figure 4 The diagram shown is a structural schematic of the bottom shell of this utility model.

[0024] Figure 5 The diagram shown is a structural schematic of the bonding pad of this utility model.

[0025] Figure 6 The diagram shows the connection of the adsorption membrane, conjugate pad, NC membrane, and absorbent filter paper of this utility model.

[0026] Component designation explanation: 1. Bottom shell; 11. Test paper mounting slot; 12. Liquid collection tank; 13. Liquid inlet tank; 14. Support platform; 2. Top shell; 21. Dropper nozzle; 22. Display window; 3. Adsorption membrane; 4. Conjugation pad; 41. Colloidal gold; 42. Injection strip; 5. NC membrane; 51. Indicator line; 6. Absorbent filter paper. Detailed Implementation

[0027] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification.

[0028] Please see Figures 1 to 6It should be understood that the structures, proportions, sizes, etc., illustrated in the accompanying drawings are merely for illustrative purposes to aid those skilled in the art and are not intended to limit the scope of this invention. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to size, without affecting the effectiveness and purpose of this invention, should still fall within the scope of the technical content disclosed in this invention. Furthermore, the terms such as "upper," "lower," "left," "right," "middle," and "one" used in this specification are merely for clarity and are not intended to limit the scope of this invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of this invention.

[0029] like Figures 1-4 As shown, this utility model provides a colloidal gold test strip for rapid detection of Seneca virus antibodies, including a bottom shell 1 and an upper shell 2. The bottom shell 1 and the upper shell 2 are snapped together to form a box and connected as a whole by a connector. The part used for detection is set inside the box. Specifically, the upper surface of the bottom shell 1 is provided with a test strip mounting groove 11. The interior of the test strip mounting groove 11 is provided with an adsorption membrane 3, a conjugate pad 4, an NC membrane 5, and a water-absorbing filter paper 6, which are limited by the test strip mounting groove 11. The adsorption membrane 3 is used to adsorb the dripped liquid sample to prevent leakage of the liquid sample, and to transport the liquid sample to the conjugate pad 4 through capillary action to bind with the labeled antibody to form a complex. The formed complex is transported to the NC membrane 5 through the conjugate pad 4, where the target antigen-gold labeled antibody complex is captured by a specific antibody for detection. The bottom of the test strip mounting groove 11 is provided with a liquid accumulation groove 12, and the side of the liquid accumulation groove 12 is provided with a liquid inlet groove 13 that communicates with the interior of the liquid accumulation groove 12. The water-absorbing filter paper 6 is located at the end of the test strip mounting groove 11 away from the liquid accumulation groove 12, and is used to adsorb excess substances in the NC membrane 5. The upper shell 2 is provided with a dropper 21 for dripping liquid samples. The dropper 21 is located directly above the liquid collection tank 12. When the absorption capacity of the adsorption membrane 3 is insufficient, the excess liquid sample will pass through the adsorption membrane 3 and directly enter the liquid collection tank 12. The binding pad 4 directly adsorbs the liquid sample from the liquid collection tank 12 through the liquid inlet 13, thereby increasing the speed at which the liquid sample binds to the labeled antibody. The upper shell 2 is provided with a long strip-shaped display window 22. The NC membrane 5 is located directly below the display window 22 for easy observation of the detection status.

[0030] The adsorption membrane 3 covers the top of the collection tank 12, and the conjugate pad 4 is in contact with the adsorption membrane 3. The bottom of the conjugate pad 4 extends into the interior of the liquid inlet tank 13. Some liquid samples exceeding the adsorption capacity of the adsorption membrane 3 will permeate through the adsorption membrane 3 into the collection tank 12 and be directly absorbed by the conjugate pad 4 through the liquid inlet tank 13, thereby increasing the binding speed of the liquid sample and the labeled antibody. One end of the NC membrane 5 is attached to the conjugate pad 4 to absorb the complex in the conjugate pad 4 through capillary action. The other end of the NC membrane 5 is attached to the absorbent filter paper 6 to absorb the migrating liquid and prevent liquid backflow.

[0031] like Figure 1 and Figure 3 As shown, in some embodiments, the shape of the drip nozzle 21 of this utility model is an inverted frustum. The axis of the frustum is provided with an inverted conical hole. The large opening facilitates the dripping of liquid samples, while the small hole at the bottom facilitates the concentrated absorption of the sample liquid by the adsorption membrane 3. This concentrates the liquid sample position and increases the absorption load of the adsorption membrane 3, thereby enabling the liquid sample to drip into the liquid collection tank 12.

[0032] It is worth noting that the adsorption membrane 3 is a thin film made of porous cellulose, which can effectively ensure uniform diffusion of the sample and good permeability.

[0033] like Figure 2 , Figure 3 and Figure 5 As shown, in some embodiments, the conjugation pad 4 of this invention includes colloidal gold 41. The colloidal gold 41 contains a chemical substance for detecting the Seneca virus VP1 protein, which is used to bind with the liquid sample. The colloidal gold 41 is Z-shaped to increase the contact area between the colloidal gold 41 and the adsorption membrane 3 and the NC membrane 5, thereby improving the migration speed of the liquid. The bottom of the colloidal gold 41 is provided with a liquid guide strip 42 with an adsorption effect. The liquid guide strip 42 extends into the liquid guide groove 13. The liquid sample flowing from the liquid accumulation groove 12 into the liquid guide groove 13 can be directly adsorbed through the liquid guide strip 42 to further improve the liquid migration speed, thereby improving the detection speed.

[0034] like Figure 2 , Figure 3 , Figure 5 and Figure 6 As shown, in some embodiments, a gap is left between the top of the liquid guide strip 42 and the bottom of the high surface of the colloidal gold 41, and the end of the adsorption membrane 3 is tightly inserted into this gap, so that both the upper and lower surfaces of the adsorption membrane 3 can contact the conjugate pad 4, thereby increasing the speed at which the sample migrates to the conjugate pad 4.

[0035] like Figure 4 and Figure 5As shown, in some embodiments, the liquid inlet groove 13 of this utility model is a plurality of strip-shaped through grooves, and the liquid inlet strip 42 corresponds one-to-one with all the liquid inlet grooves 13, which is used to increase the contact area between the liquid inlet strip 42 and the liquid inlet groove 13, and to further improve the migration speed of the liquid sample.

[0036] like Figure 2 and Figure 4 As shown, in some embodiments, the bottom of the test strip mounting groove 11 of this utility model is provided with a support platform 14 to raise the depth of the test strip mounting groove 11. The combination pad 4 and the absorbent filter paper 6 are respectively located on both sides of the support platform 14. The NC membrane 5 is placed on the top of the support platform 14. The thickness of the NC membrane 5 can be reduced by the support of the support platform 14. By reducing the thickness of the NC membrane 5, the diffusion range of the liquid can be reduced, so that the liquid can migrate through the NC membrane 5 more quickly.

[0037] like Figure 3 As shown, in some embodiments, the NC membrane 5 of this invention is provided with two indicator lines 51, which are a test line and a control line, respectively. Both the test line and the control line are located within the viewing window of the display window 22, and the test line is marked as the T line and the control line is marked as the C line. The color development is used to enhance positive results and verify the effectiveness of the test strip.

[0038] like Figure 3 and Figure 6 As shown, in some embodiments, the top side of the absorbent filter paper 6 of this invention facing the NC membrane 5 is provided with a groove, and the end of the NC membrane 5 overlaps the groove. The groove can increase the contact area between the NC membrane 5 and the absorbent filter paper 6, so that the absorbent filter paper 6 can adsorb the migrating liquid more quickly during the detection process and avoid liquid backflow.

[0039] In summary, the colloidal gold test strip for rapid detection of Seneca virus antibodies of this invention improves detection efficiency by setting a liquid accumulation groove 12 corresponding to the position of the drop outlet 21 at the bottom of the test strip mounting groove 11, and setting a liquid inlet groove 13 on the side of the liquid accumulation groove 12 so that the liquid inlet strip 42 extends into the interior of the liquid inlet groove 13. At the same time, the liquid accumulation groove 12 is covered by an adsorption membrane 3. After the liquid sample is dripped in through the drop outlet 21, the adsorption membrane 3 adsorbs the sample liquid through capillary action. Part of the sample liquid is transported to the colloidal gold 41 through the adsorption membrane 3, and part of the sample liquid permeates into the liquid accumulation groove 12 and is directly adsorbed by the liquid inlet strip 42. Finally, it is transported to the NC membrane 5 through the colloidal gold 41.

[0040] This invention improves detection efficiency by arranging the colloidal gold 41 in a Z-shape and creating a gap between the top of the liquid guide strip 42 and the bottom of the high surface of the colloidal gold 41, allowing the adsorption membrane 3 to be inserted. This enables the sample liquid adsorbed by the adsorption membrane 3 to be transported to the colloidal gold 41 through both the top and bottom surfaces.

[0041] This invention supports the NC membrane 5 by setting a support platform 14 in the test strip mounting groove 11, thereby reducing the thickness of the NC membrane 5 and enabling the combined liquid to be transported in the NC membrane 5 more quickly. At the same time, it can also reduce the dosage of the test sample.

[0042] Therefore, this utility model effectively overcomes the various shortcomings of the prior art and has high industrial application value.

[0043] The above embodiments are merely illustrative of the principles and effects of this utility model and are not intended to limit the scope of this utility model. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of this utility model. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this utility model should still be covered by the claims of this utility model.

Claims

1. A colloidal gold test strip for rapid detection of Seneca virus antibodies, characterized in that, It includes a bottom shell (1) and an upper shell (2), which are fastened together to form a box body; The upper surface of the bottom shell (1) is provided with a test paper mounting groove (11). The interior of the test paper mounting groove (11) is provided with an adsorption membrane (3), a binding pad (4), an NC membrane (5), and a water-absorbing filter paper (6). The bottom of the test paper mounting groove (11) is provided with a liquid accumulation groove (12). The side of the liquid accumulation groove (12) is provided with a liquid guiding groove (13). The water-absorbing filter paper (6) is located at the end of the test paper mounting groove (11) away from the liquid accumulation groove (12). The upper shell (2) is provided with a drip port (21), which is located directly above the liquid accumulation tank (12). The upper shell (2) is provided with a long strip display window (22), and the NC membrane (5) is located directly below the display window (22). The adsorption membrane (3) covers the top of the liquid collection tank (12), the binding pad (4) abuts against the adsorption membrane (3), and the bottom of the binding pad (4) extends into the interior of the liquid collection tank (13). One end of the NC membrane (5) overlaps the binding pad (4), and the other end of the NC membrane (5) overlaps the absorbent filter paper (6).

2. The colloidal gold test strip for rapid detection of Seneca virus antibodies according to claim 1, characterized in that: The drip nozzle (21) is shaped like an inverted frustum, with an inverted conical hole at the center of the frustum.

3. The colloidal gold test strip for rapid detection of Seneca virus antibodies according to claim 1, characterized in that: The adsorption membrane (3) is a thin film made of porous cellulose.

4. The colloidal gold test strip for rapid detection of Seneca virus antibodies according to claim 1, characterized in that: The bonding pad (4) includes colloidal gold (41), which is Z-shaped, and the bottom of the colloidal gold (41) is provided with a liquid guide strip (42), which extends into the interior of the liquid guide groove (13).

5. The colloidal gold test strip for rapid detection of Seneca virus antibodies according to claim 4, characterized in that: A gap is left between the top of the liquid guide strip (42) and the bottom of the high surface of the colloidal gold (41), and the end of the adsorption membrane (3) is tightly inserted into this gap.

6. The colloidal gold test strip for rapid detection of Seneca virus antibodies according to claim 5, characterized in that: The liquid inlet groove (13) is a number of strip-shaped through grooves, and the liquid inlet strip (42) corresponds one-to-one with all the liquid inlet grooves (13).

7. The colloidal gold test strip for rapid detection of Seneca virus antibodies according to claim 1, characterized in that: The bottom of the test paper mounting groove (11) is provided with a support platform (14), the bonding pad (4) and the absorbent filter paper (6) are located on both sides of the support platform (14), and the NC membrane (5) is placed on the top of the support platform (14).

8. The colloidal gold test strip for rapid detection of Seneca virus antibodies according to claim 1, characterized in that: The NC membrane (5) is provided with two indicator lines (51), which are a test line and a quality control line, respectively. Both the test line and the quality control line are located within the viewing window of the display window (22).

9. The colloidal gold test strip for rapid detection of Seneca virus antibodies according to claim 1, characterized in that: The absorbent filter paper (6) has a groove on the side facing the NC membrane (5), and the end of the NC membrane (5) overlaps the groove.

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