A processing device for fixing environmental microorganism DNA on a water body filter membrane

Abstract

The utility model discloses a kind of processing device of water body filter membrane fixed environmental microorganism DNA, including the pretreatment box of being equipped with several placing cavities and the collection box with refrigeration component in inner cavity, pre-treatment pipe is placed in placing cavity and placing cavity lower end is equipped with liquid outlet passage, refrigeration component upper side is connected with the metal containing body of being equipped with several containing cavities, containing cavity contains centrifuge tube, the assembly plate of being located centrifuge tube upper side is covered on the upper end cover of collection box, assembly plate includes the assembly hole of being connected with the liquid outlet passage and centrifuge tube and the fixing column in assembly hole inner cavity, fixing column is fixedly connected with the stainless steel needle that can penetrate liquid outlet passage and pre-treatment pipe.The utility model can synchronously process multiple samples at a time, shorten the processing time of batch samples, improve the processing efficiency and collection efficiency of microorganism DNA sample, the temperature of centrifuge tube in containing cavity can be stably controlled at 4 ℃, realize the rapid, batch, stable processing of water body filter membrane fixed environmental microorganism DNA.

Description

Technical Field

[0001] This utility model relates to the field of microbial DNA treatment technology, and in particular to a treatment device for fixing environmental microbial DNA using a water filter membrane. Background Technology

[0002] The ecological environment of rivers, lakes, and seas is a complex and fragile ecosystem, playing a crucial role in maintaining biodiversity, water cycles, and ecological balance. Therefore, microbial monitoring of the aquatic environment has become a vital component of environmental protection work. Compared to physicochemical methods, aquatic microbial DNA testing can more sensitively and accurately express the effects of different influencing factors and pollutants. Consequently, aquatic microbial DNA testing is widely used in taxonomic studies of aquatic animals, plants, zooplankton, benthic organisms, and aquatic microorganisms, as well as in genetic analysis of biological gene functions. In aquatic microbial monitoring, processing the environmental microbial DNA fixed on water filter membranes is a key step; its processing efficiency and sample stability directly affect the accuracy of subsequent aquatic ecological identification results.

[0003] Currently, existing microbial DNA processing devices have several shortcomings: 1. Traditional processing devices are mostly integrated designs, requiring manual operation for reagent addition and sample transfer in separate steps. They can only process 1-2 samples at a time, resulting in complex and time-consuming procedures that fail to meet the needs of batch sample processing and lead to low sample processing efficiency. 2. Microbial DNA samples are sensitive to storage temperature. Existing devices lack specific temperature control structures, making samples prone to degradation due to temperature fluctuations during processing, leading to a decrease in DNA extraction rate and affecting subsequent detection. 3. Reagents are mostly manually dispensed on-site, which can easily cause dosage deviations. Furthermore, the connectivity between reagents and centrifuge tubes is unstable after reagent addition, potentially leading to leakage of lysis buffer or incomplete sample transfer, resulting in poor sample processing quality. Utility Model Content

[0004] To address the technical problems of low sample processing efficiency, easy degradation of samples due to temperature fluctuations during processing, and poor sample processing quality in existing technologies, this utility model provides the following technical solution.

[0005] This utility model discloses a treatment device for fixing environmental microbial DNA using a water filter membrane. It includes a pretreatment box with several placement chambers and a collection box with a cooling component inside. Pretreatment tubes are placed in the placement chambers, and a liquid outlet channel is provided at the lower end of each placement chamber. A metal container with several receiving chambers is connected above the cooling component. Centrifuge tubes are contained within the receiving chambers. An assembly plate is fitted over the upper end of the collection box, located above the centrifuge tubes. The assembly plate includes an assembly hole connecting the liquid outlet channel and the centrifuge tubes, and a fixing column located within the assembly hole. A stainless steel needle that can penetrate the liquid outlet channel and the pretreatment tube is fixedly connected to the fixing column.

[0006] As a further technical solution, a matching tube is fixedly connected to the lower end of the liquid outlet channel, and a sealing collar matching the outer wall of the matching tube is provided in the assembly hole at the upper part of the fixed column.

[0007] As a further technical solution, the cooling component includes a semiconductor cooling chip, a heat sink connected to the lower part of the semiconductor, and a cooling fan. The upper part of the semiconductor cooling chip is connected to a heat-conducting plate and a secondary heat-conducting plate.

[0008] As a further technical solution, the upper periphery of the assembly plate is provided with positioning holes, and the lower periphery of the pretreatment box is provided with limiting posts that match the positioning holes.

[0009] As a further technical solution, the assembly plate is provided with a snap-in edge that matches the collection box, and a second handle is provided on both sides of the assembly plate.

[0010] As a further technical solution, the upper end of the pretreatment tube is sealed with an easy-tear film.

[0011] As a further technical solution, the pretreatment box is provided with a first handle on both sides.

[0012] The beneficial effects of this invention are as follows: The pretreatment box of this invention can hold multiple pretreatment tubes, which are pre-filled with lysis reagent, eliminating the need for manual on-site dispensing and reducing operational steps. The collection box contains multiple centrifuge tubes corresponding to the pretreatment tubes for sample collection, allowing the pretreatment box and collection box to process multiple samples simultaneously, significantly shortening the processing time for batch samples. Furthermore, the lysed samples automatically flow into the centrifuge tubes without manual transfer, reducing operational difficulty and improving the processing and collection efficiency of microbial DNA samples. Simultaneously, the collection box is equipped with a cooling component that stably controls the temperature of the centrifuge tubes within the chamber at 4°C, effectively inhibiting the activity of DNA-degrading enzymes and preventing sample degradation during processing, ensuring the integrity and stability of the DNA. This enables rapid, batch, and stable processing of microbial DNA from water filter membrane immobilization environments, providing reliable specimens for subsequent aquatic ecosystem identification. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the external structure of the water body filter membrane for fixing the DNA of environmental microorganisms according to this utility model.

[0014] Figure 2 This is a schematic diagram of the water body filter membrane for fixing environmental microbial DNA after the cover is opened. Figure 3 yes Figure 2 Bottom diagram; Figure 4 This is a cross-sectional schematic diagram of the water body filter membrane for fixing the DNA of environmental microorganisms according to this utility model; Figure 5 This is a cross-sectional view of the assembly plate of the water body filter membrane for fixing the DNA of environmental microorganisms according to this utility model; In the diagram: 1-Pretreatment box; 101-First top cover; 102-First handle; 103-Placement cavity; 104-Liquid outlet channel; 105-Limiting post; 2-Collection box; 201-Second top cover; 202-Support leg; 3-Pretreatment tube; 301-Easy-tear film; 4-Matching tube; 5-Refrigeration component; 501-Semiconductor cooling chip; 502-Heat sink; 503-Cooling fan; 504-Cooling fin; 505-Secondary cooling fin; 6-Metal container; 601-Containing cavity; 7-Centerprise tube; 8-Assembly plate; 801-Assembly hole; 802-Fixing post; 803-Stainless steel needle; 804-Sealing collar; 805-Positioning hole; 806-Second handle; 807-Snap-in edge. Detailed Implementation

[0015] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model. It should be noted that, unless otherwise specified, the embodiments and features described herein can be combined with each other.

[0016] In the description of this utility model, it should be understood that the terms "upper" and "lower" are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0017] like Figure 1 As shown, this utility model discloses a treatment device for fixing environmental microbial DNA on a water filter membrane, comprising a pretreatment tank 1 and a collection tank 2. The pretreatment tank 1 is used to pretreat the water filter membrane, and the collection tank 2 is used to collect the treated lysate containing microbial DNA. The pretreatment tank 1 is provided with first handles 102 on both sides for easy lifting and connection to the collection tank 2. A first top cover 101 and a second top cover 201 are respectively fitted onto the upper parts of the pretreatment tank 1 and the collection tank 2. The first top cover 101 and the second top cover 201 can be hinged to the upper parts of the pretreatment tank 1 and the collection tank 2, respectively. Alternatively, the first top cover 101 and the second top cover 201 can simply cover the upper parts of the pretreatment tank 1 and the collection tank 2; this utility model does not impose any particular limitation on this.

[0018] like Figure 2 and Figure 4 As shown, in a preferred embodiment, the pretreatment chamber 1 is provided with multiple placement chambers 103, each placement chamber 103 having a liquid outlet channel 104 at its lower end for the discharge of the treated lysis solution. In this embodiment, there are nine placement chambers 103, but other numbers are also possible. A pretreatment tube 3 is placed inside each placement chamber 103, and the pretreatment tube 3 contains pre-filled lysis reagent. The upper end of the pretreatment tube 3 is sealed with an easy-tear film 301. In use, the easy-tear film 301 at the upper end of the pretreatment tube 3 is torn open, and the water filter membrane is placed into the lysis solution in the pretreatment tube 3 for about 10 minutes to obtain a lysis solution containing microbial DNA. This eliminates the need for manual dispensing of the lysis solution on-site, reducing the operational steps in the microbial DNA treatment process.

[0019] like Figure 3 and Figure 4 As shown, in a preferred embodiment, the inner cavity of the collection box 2 is provided with a cooling component 5. The cooling component 5 is used to store the lysed solution containing microbial DNA at low temperature. It can stably control the temperature environment of the lysed solution at 4°C, which can effectively inhibit the activity of DNA degrading enzymes, prevent the sample from being degraded during the processing, and ensure the integrity and stability of the DNA.

[0020] Specifically, the cooling component 5 includes a thermoelectric cooler 501 and a heat sink 502 connected to the lower part of the thermoelectric cooler 501. The thermoelectric cooler 501 is connected to an external power supply and a temperature controller via wires. Temperature sensors are connected to both the collection box 2 and the metal container 6 for real-time temperature monitoring. A cooling fan 503 is connected to the lower part of the heat sink 502 via thermal grease, and the cooling fan 503 extends to the bottom plate of the collection box 2. At this time, the bottom of the collection box 2 is provided with multiple support legs 202. A cooling plate 504 is connected to the upper part of the thermoelectric cooler 501. A secondary cooling plate 505 is connected to the upper part of the cooling plate 504 via thermal grease. The secondary cooling plate 505 is connected to the metal container 6, which has multiple receiving cavities 601. The metal container 6 is connected to the secondary cooling plate 505 and fixedly connected to the side wall of the collection box 2. The number of receiving cavities 601 is the same as the number of placement cavities 103, and they correspond one-to-one in the longitudinal direction. At this time, the containment cavity 601 contains centrifuge tubes 7, which are used to collect the lysed solution containing microbial DNA.

[0021] like Figure 4 and Figure 5 As shown, in a preferred embodiment, the upper end of the collection box 2 is covered by an assembly plate 8 located above the centrifuge tube 7. The assembly plate 8 has snap-fit ​​edges 807 that match the inner wall of the collection box 2, and second handles 806 are provided on both sides of the assembly plate 8, thereby allowing the assembly plate 8 to be placed on the upper part of the collection box 2. The pretreatment box 1 is connected to the collection box 2 through the assembly plate 8. After connection, the lysed solution containing microbial DNA in the pretreatment box 1 can enter the centrifuge tube 7. A matching tube 4 is fixedly connected to the lower end of the outlet channel 104. The inner diameter of the matching tube 4 is not less than the inner diameter of the outlet channel 104. The assembly plate 8 includes an assembly hole 801 for connecting the matching tube 4. The upper part of the assembly hole 801 is connected to the matching tube 4, and the lower part of the assembly hole 801 is connected to the centrifuge tube 7. The lysed solution flows from the outlet channel 104 into the centrifuge tube 7 through the matching tube 4 and the assembly hole 801.

[0022] At this time, a horizontally positioned fixing post 802 is fixedly installed in the lower part of the inner cavity of the assembly hole 801. A stainless steel needle 803 that can pass through the liquid outlet channel 104 and the pretreatment tube 3 is fixedly connected in the middle of the fixing post 802. The stainless steel needle 803 can be solid or hollow. When the stainless steel needle 803 is hollow, its upper and lower ends are connected. The first upper cover 101 is placed on the upper end of the pretreatment box 1 and held, and the pretreatment box 1 is placed on the upper part of the collection box 2. During the downward movement of the pretreatment box 1, the stainless steel needle 803 will pass through the matching tube 4 and the liquid outlet channel 104, and the stainless steel needle 803 can puncture the pretreatment tube 3. The pyrolysis solution in the punctured pretreatment tube 3 will flow into the centrifuge tube 7.

[0023] In a preferred embodiment, to ensure the accurate positioning of the pretreatment box 1 and the collection box 2, four positioning holes 805 are provided around the upper periphery of the assembly plate 8, and four limiting posts 105 matching the positioning holes 805 are provided around the lower periphery of the pretreatment box 1. When the pretreatment box 1 and the cover are closed on the upper part of the assembly plate 8, the limiting posts 105 are inserted into the positioning holes 805. At this time, the stainless steel needle 803 just pierces the lower end of the pretreatment tube 3 without penetrating too deeply, ensuring that the pyrolysis solution flows smoothly into the centrifuge tube 7. At the same time, a sealing ring 804 matching the outer wall of the matching tube 4 is provided in the assembly hole 801 above the fixing post 802. The sealing ring 804 is an O-ring seal, which can prevent the pyrolysis solution from leaking out from the connection between the assembly hole 801 and the matching tube 4.

[0024] In use, firstly, open the first top cover 101 and tear the easy-tear film 301 at the top of the pretreatment tube 3. Place the multiple water filter membrane samples to be treated into each pretreatment tube 3. The water filter membrane samples come into contact with the lysis reagent in the pretreatment tube 3 and begin the lysis reaction, releasing microbial DNA. At the same time, activate the cooling component 5 in the collection box 2 for cooling. After the lysis reaction is complete, hold the first handles 102 on both sides of the pretreatment box 1 and move the pretreatment box 1 above the collection box 2, so that the multiple matching tubes 4 at the bottom of the pretreatment tube 3 are aligned with each assembly hole of the assembly plate 8. 801 and move downwards; after the limiting post 105 is fully inserted into the positioning hole 805, the stainless steel needle 803 passes through the matching tube 4 and the liquid outlet channel 104 to puncture the pretreatment tube 3. The lysis solution in the punctured pretreatment tube 3 flows from the liquid outlet channel 104 through the matching tube 4 and the assembly hole 801 into the centrifuge tube 7; then, the cooling component 5 in the collection box 2 continues to work to stabilize the internal temperature of the collection box 2 at 4°C, ensuring that the sample in the centrifuge tube 7 is in a low-temperature preservation state. After all the samples have been transferred, the assembly plate 8 is lifted, and the centrifuge tube 7 can be directly removed for subsequent processing, or it can continue to be stored in the collection box 2.

[0025] The preferred embodiments and examples of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments and examples. Within the scope of knowledge possessed by those skilled in the art, various changes or equivalent substitutions can be made without departing from the concept of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the scope of protection of the present invention.

Claims

1. A treatment device for fixing environmental microbial DNA using a water filter membrane, characterized in that: The system includes a pretreatment box (1) with several placement chambers (103) and a collection box (2) with a refrigeration component (5) in its inner cavity. The placement chamber (103) contains a pretreatment tube (3) and the lower end of the placement chamber (103) has a liquid outlet channel (104). The refrigeration component (5) is connected above a metal container (6) with several receiving chambers (601). The receiving chamber (601) contains a centrifuge tube (7). The upper end of the collection box (2) is covered by an assembly plate (8) located above the centrifuge tube (7). The assembly plate (8) includes an assembly hole (801) connecting the liquid outlet channel (104) and the centrifuge tube (7) and a fixing post (802) located in the inner cavity of the assembly hole (801). The fixing post (802) is fixedly connected with a stainless steel needle (803) that can penetrate the liquid outlet channel (104) and the pretreatment tube (3).

2. The treatment device for fixing environmental microbial DNA using a water filter membrane according to claim 1, characterized in that: The lower end of the liquid outlet channel (104) is fixedly connected to a matching tube (4), and a sealing collar (804) matching the outer wall of the matching tube (4) is provided in the assembly hole (801) above the fixed column (802).

3. The treatment device for fixing environmental microbial DNA using a water filter membrane according to claim 1, characterized in that: The cooling component (5) includes a semiconductor cooling chip (501), a heat sink (502) connected to the lower part of the semiconductor (501), and a cooling fan (503). A heat-conducting plate (504) and a secondary heat-conducting plate (505) are connected to the upper part of the semiconductor cooling chip (501).

4. The treatment device for fixing environmental microbial DNA using a water filter membrane according to claim 1, characterized in that: The upper periphery of the assembly plate (8) is provided with positioning holes (805), and the lower periphery of the pretreatment box (1) is provided with limiting posts (105) that match the positioning holes (805).

5. The treatment device for fixing environmental microbial DNA using a water filter membrane according to claim 1, characterized in that: The assembly plate (8) is provided with a snap-in edge (807) that matches the collection box (2), and a second handle (806) is provided on both sides of the assembly plate (8).

6. The treatment device for fixing environmental microbial DNA using a water filter membrane according to claim 1, characterized in that: The upper end of the pretreatment tube (3) is sealed with an easy-tear film (301).

7. The treatment device for fixing environmental microbial DNA using a water filter membrane according to claim 1, characterized in that: The pretreatment box (1) is provided with a first handle (102) on both sides.

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