Cell thawing apparatus

Abstract

The utility model provides a kind of cell recovery fluid washing device, the device can be input cell recovery fluid and cell washing fluid to inlet by inlet pipeline first, two kinds of liquid are mixed in cell recovery fluid washing bag after flowing to cut-off membrane, after the cut-off effect of cut-off membrane, cell is retained on cut-off membrane, waste liquid flows to washing function pipeline from inlet and outlet, after all waste liquid flows out, resuspension liquid is input to inlet and outlet again by washing function pipeline, reverse flush cell on cut-off membrane, cell will flow to outlet pipeline by outlet, finally obtain washed cell. That is, the cell recovery fluid washing device of the utility model can automatically wash cell recovery fluid by three kinds of pipeline and closed cell recovery fluid washing bag, the washing process is closed operation without opening, the requirement of relative low to environmental cleanliness, and reduce manpower and material resources cost, improve washing efficiency.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a cell resuscitation fluid washing device. Background Technology

[0002] The biopharmaceutical industry requires maintaining high levels of sterility during production. Based on current equipment selection in the biopharmaceutical industry, biosafety cabinets are typically used to maintain a localized Class A ventilation environment. The preparation of HepaCure plasma bio-purification columns requires hiHep cells to undergo resuscitation and washing before clinical application. Currently, there are no specialized hiHep cell resuscitation solution washing devices on the market, so manual operation is mainly used. This involves manual centrifugation and resuspension, requiring four centrifugation and three resuspension steps, which is time-consuming, demands high skill levels from personnel, and, for safety reasons, must be performed in a Class A environment, hindering large-scale industrial application.

[0003] Therefore, the existing cell resuscitation fluid washing process suffers from technical problems such as low efficiency, high labor costs, and high requirements for environmental cleanliness, and needs to be improved. Utility Model Content

[0004] This embodiment provides a cell resuscitation fluid washing device to solve the technical problems of low efficiency, high labor costs, and high requirements for environmental cleanliness in the current cell resuscitation fluid washing process.

[0005] To address the aforementioned technical problems, this embodiment provides the following technical solution:

[0006] This embodiment provides a cell resuscitation fluid washing device, including a cell resuscitation fluid washing bag, an inlet pipe, a washing function pipe, and an outlet pipe. The cell resuscitation fluid washing bag includes a first side and a second side arranged opposite to each other. The first side is provided with an inlet and an outlet, and the second side is provided with an inlet and an outlet. A retaining membrane is provided between the first side and the inlet and outlet. The inlet is connected to the inlet pipe, the outlet is connected to the outlet pipe, and the inlet and outlet are connected to the washing function pipe.

[0007] In one embodiment, the cell resuscitation fluid washing bag further includes a plastic filter screen disposed between the first side and the inlet / outlet, and the retention membrane is adhered to the plastic filter screen.

[0008] In one embodiment, the second orthographic projection of the inlet / outlet on the retention membrane is located between the first orthographic projection of the inlet on the retention membrane and the third orthographic projection of the outlet on the retention membrane.

[0009] In one embodiment, the liquid inlet pipeline includes a first single connector, a first connecting pipe, a first reducing connector, a second connecting pipe, a first Luer connector, and a first Luer cap connected in sequence. A first clamping member is provided outside the first connecting pipe, and the first single connector is connected to the liquid inlet.

[0010] In one embodiment, the second connecting pipe is connected to the first peristaltic pump.

[0011] In one embodiment, the washing function pipeline includes a second single connector, a third connecting pipe, a second reducing connector, a fourth connecting pipe, a first equal diameter connector, a first peristaltic pump pipe, a second equal diameter connector, a first thermoplastic pipe, a second Luer connector, and a second Luer cap, which are connected in sequence. A second clamping member is provided on the outside of the third connecting pipe, and the second single connector is connected to the inlet and outlet.

[0012] In one embodiment, the first peristaltic pump tube is connected to the second peristaltic pump.

[0013] In one embodiment, the liquid outlet pipeline includes a third single connector, a fifth connecting pipe, a third reducing connector, a sixth connecting pipe, a third equal diameter connector, a second peristaltic pump pipe, a fourth equal diameter connector, a second thermoplastic pipe, a third Luer connector, and a third Luer cap connected in sequence. A third clamping member is provided outside the fifth connecting pipe, and the third single connector is connected to the liquid outlet.

[0014] In one embodiment, the second peristaltic pump tube is connected to the third peristaltic pump.

[0015] In one embodiment, the liquid outlet line is connected to a cell filling device.

[0016] The positive and progressive effects of this utility model are as follows:

[0017] This embodiment provides a cell resuscitation fluid washing device. The device first introduces cell resuscitation fluid and cell washing fluid into the inlet through an inlet pipe. The two liquids mix in the cell resuscitation fluid washing bag and then flow to a retention membrane. The cells are retained on the retention membrane, and the waste liquid flows from the inlet / outlet to the washing function pipe. After all the waste liquid has flowed out, a resuspension solution is introduced into the inlet / outlet through the washing function pipe to backwash the cells on the retention membrane. The cells then flow out through the outlet to the outlet pipe, ultimately yielding washed cells. In other words, this cell resuscitation fluid washing device can automatically wash cell resuscitation fluid using three types of pipes and a sealed cell resuscitation fluid washing bag. The washing process is a closed operation with no openings, requiring relatively low environmental cleanliness, reducing labor and material costs, and improving washing efficiency. Attached Figure Description

[0018] Figure 1This is an exploded structural diagram of the cell resuscitation fluid washing device provided in an embodiment of the present invention.

[0019] Figure 2 The diagram shows the front and back of the cell resuscitation fluid washing bag provided in this embodiment of the invention.

[0020] Explanation of reference numerals in the attached figures

[0021] Cell resuscitation fluid wash bag 100

[0022] Inlet pipe 10

[0023] Washing function piping 20

[0024] 30mm outlet pipe

[0025] First side 200

[0026] Second side 300

[0027] Inlet 1

[0028] Inlet / outlet 2

[0029] Liquid outlet 3

[0030] Retaining membrane 4

[0031] Plastic filter screen 5

[0032] First length L1

[0033] First width W1

[0034] Second length L2

[0035] Second width W2

[0036] Spacing L3

[0037] First single connector 101

[0038] First connecting pipe 102

[0039] First reducing joint 103

[0040] Second connecting pipe 104

[0041] First Ruhr Connector 105

[0042] First Ruhr Helmet 106

[0043] First clamping component 107

[0044] First self-sealing bag 108

[0045] Second single connector 201

[0046] Third connecting pipe 202

[0047] Second reducing joint 203

[0048] Fourth connecting pipe 204

[0049] First equal diameter connector 205

[0050] First peristaltic pump tube 206

[0051] Second equal diameter connector 207

[0052] First thermoplastic pipe 208

[0053] Second Ruhr Connector 209

[0054] Second Ruhr Helmet 210

[0055] Second clamping component 211

[0056] Second self-sealing bag 212

[0057] Third single connector 301

[0058] Fifth connecting pipe 302

[0059] Third reducing connector 303

[0060] Sixth connecting pipe 304

[0061] Third equal diameter connector 305

[0062] Second peristaltic pump tube 306

[0063] Fourth equal diameter connector 307

[0064] Second thermoplastic tube 308

[0065] Third Ruhr connector 309

[0066] Third Ruhr Helmet 310

[0067] Third clamping component 311

[0068] Third self-sealing bag 312

[0069] Cable ties 400 Detailed Implementation

[0070] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present utility model. In addition, it should be understood that the specific embodiments described herein are only for illustration and explanation of the present utility model and are not intended to limit the present utility model. In the present utility model, unless otherwise stated, directional terms such as "upper" and "lower" generally refer to the upper and lower positions of the device in actual use or operation, specifically the drawing directions in the accompanying drawings; while "inner" and "outer" refer to the outline of the device.

[0071] Combination such as Figure 1 and Figure 2 As shown, the cell resuscitation fluid washing device includes a cell resuscitation fluid washing bag 100, an inlet pipe 10, a washing function pipe 20, and an outlet pipe 30. The cell resuscitation fluid washing bag 100 includes a first side 200 and a second side 300 arranged opposite to each other. The first side 200 is provided with an inlet 1 and an outlet 3, and the second side 300 is provided with inlet and outlet 2. A retaining membrane 4 is provided between the first side 200 and the second side 300. The inlet 1 is connected to the inlet pipe 10, the outlet 3 is connected to the outlet pipe 30, and the inlet and outlet 2 are connected to the washing function pipe 20.

[0072] Figure 2 Image (a) is a schematic diagram of the front structure of the cell resuscitation fluid washing bag. Figure 2 (b) is a schematic diagram of the reverse side structure of the cell resuscitation fluid washing bag. The cell resuscitation fluid washing bag 100 is a disposable sterile bag with a capacity of 1 liter or other specifications. Taking a capacity of 1 liter as an example, the first length L1 of the cell resuscitation fluid washing bag 100 can be 280 mm, and the first width W1 can be 180 mm. The front side shows the first side 200 of the cell resuscitation fluid washing bag 100, and the reverse side shows the second side 300 of the cell resuscitation fluid washing bag 100. The first side 200 has an inlet 1 and an outlet 3, and the second side 300 has an inlet and outlet 2. When all three liquid outlets are closed, the entire bag forms a closed space. The retention membrane 4 is located inside the cell resuscitation fluid washing bag 100, specifically between the first side 200 and the inlet and outlet 2. There are gaps between the retention membrane 4 and both the first side 200 and the second side 300. The function of the retention membrane 4 is to retain hiHep cells and allow other liquids to flow through.

[0073] The first end of the inlet pipe 10 is connected to the inlet port 1, and the second end of the inlet pipe 10 can be connected to the cell resuscitation fluid supply device and the cell washing fluid supply device respectively. First, the cell resuscitation fluid in the cell resuscitation fluid supply device is introduced into the inlet port 1, and then the cell washing fluid in the cell washing fluid supply device is introduced into the inlet port 1. The two liquids will mix in the gap between the retention membrane 4 and the first side 200. After the retention effect of the retention membrane 4, the cells are retained on the retention membrane 4, and the waste liquid flows through the retention membrane 4 to the other side of the retention membrane 4.

[0074] The first end of the washing function pipeline 20 is connected to the inlet / outlet 2. The second end of the washing function pipeline 20 is first connected to the waste liquid storage device. Waste liquid can flow through the inlet / outlet 2 to the washing function pipeline 20 and enter the waste liquid storage device. After the waste liquid has completely flowed out, the second end of the washing function pipeline 20 is then connected to the resuspension supply device. The resuspension in the resuspension supply device flows through the inlet / outlet 2 to the retention membrane 4, backwashing the cells on the retention membrane 4 and causing them to enter the gap between the retention membrane 4 and the first side 200.

[0075] The first end of the outlet pipe 30 is connected to the outlet 3, and the second end of the outlet pipe 30 is connected to the cell storage device. Cells in the gap between the retention membrane 4 and the first side 200 can flow into the outlet pipe 30 through the outlet 3 and eventually enter the cell storage device.

[0076] As can be seen from the above, the cell resuscitation fluid washing device of this utility model can automatically wash the cell resuscitation fluid through three types of pipelines and a closed cell resuscitation fluid washing bag. The washing process is a closed operation without openings, which has relatively low requirements for environmental cleanliness. It can be completed in a Class C environment, and it reduces labor and material costs, improves washing efficiency, effectively reduces impurity residue, and is conducive to ensuring the sterility of the product.

[0077] In one embodiment, the cell resuscitation fluid washing bag 100 further includes a plastic filter 5, which is disposed between the first side 200 and the inlet / outlet 2. A retaining membrane 4 is adhered to the plastic filter 5. Because the retaining membrane 4 is relatively soft, if it were only disposed between the first side 200 and the inlet / outlet 2, it might adhere to the bag body, obstructing the flow of liquid. In this embodiment, the retaining membrane 4 is adhered to the plastic filter 5. Since the plastic filter 5 is relatively rigid, the retaining membrane 4 can be smoothly embedded in the bag body, avoiding complete adhesion and ensuring smoother inflow and outflow of liquid. The retaining membrane 4 and the plastic filter 5 can be the same size, such as... Figure 2As shown, if the first length L1 of the cell resuscitation fluid washing bag 100 is 280 mm and the first width W1 is 180 mm, then the second length L2 of the retention membrane 4 and the plastic filter 5 can be 220 mm and the second width W2 can be 120 mm. Furthermore, to avoid affecting the performance of the retention membrane 4, the pore size of the plastic filter 5 will be larger than that of the retention membrane 4; the pore size of the retention membrane 4 is typically in the micrometer range.

[0078] In one embodiment, the second orthographic projection of the inlet / outlet 2 onto the retention membrane 4 is located between the first orthographic projection of the inlet 1 onto the retention membrane 4 and the third orthographic projection of the outlet 3 onto the retention membrane 4. For example... Figure 2 As shown, viewed from either the front or back, the inlet and outlet 2 are both located between the inlet 1 and the outlet 3, and the distance L3 between the inlet 1 and the outlet 3 can be 160mm. This arrangement ensures that the input and output of various liquids do not interfere with each other, improving washing efficiency and quality.

[0079] In one embodiment, the liquid inlet pipeline 10 includes a first single connector 101, a first connecting pipe 102, a first reducing connector 103, a second connecting pipe 104, a first Luer connector 105, and a first Luer cap 106 connected in sequence. A first clamping member 107 is provided outside the first connecting pipe 102, and the first single connector 101 is connected to the liquid inlet 1.

[0080] like Figure 1 As shown, the first single connector 101 is a 1 / 4" single connector, the first connecting tube 102 is a 3 / 16" (4.8*8) medical silicone tube with a length of 60mm, the first reducing connector 103 is a 1 / 4" to 1 / 8" reducing connector, the second connecting tube 104 is a 1 / 8" (3.2*6.4) medical silicone tube with a length of 500mm, the first Luer connector 105 is a 1 / 8" female Luer connector, and the first Luer cap 106 is a female Luer cap.

[0081] The first Luer connector 105 can be directly connected to various commercially available disposable liquid bag interfaces for liquid transfer. Figure 1 These liquid bags are collectively referred to as the first self-sealing bag 108. The first self-sealing bag 108 can be a cell resuscitation fluid supply unit, a cell washing fluid supply unit, or other functional components, depending on the requirements. When the inlet pipe 10 is not connected to the first self-sealing bag 108, the first Luer cap 106 is connected to the first Luer connector 105 to protect the inlet pipe 10 from contamination. When it is necessary to connect it to the first self-sealing bag 108, the first Luer cap 106 is first removed, and then the first Luer connector 105 is connected to the first self-sealing bag 108. The above components can be connected by cable ties 400, or they can be nested with adjacent components based on their shape.

[0082] A first clamping member 107 is provided outside the first connecting tube 102. The first clamping member 107 can be a 1 / 4" Y-shaped clamp. When liquid needs to be introduced, the first connecting tube 102 can be adjusted to be located in the wide slot of the first clamping member 107 to keep the liquid inside the first connecting tube 102 flowing relatively smoothly. When liquid does not need to be introduced or the liquid introduction speed needs to be slowed down, the first connecting tube 102 can be adjusted to be stuck in the narrow slot of the first clamping member 107, so that the liquid inside the first connecting tube 102 flows slowly or stops flowing.

[0083] In one embodiment, the second connecting tube 104 is connected to the first peristaltic pump. After the first peristaltic pump is connected to the second connecting tube 104, it can control the automatic entry of cell resuscitation fluid and cell washing fluid into the inlet 1. The flow rate of the liquid can be adjusted to any value between 0 and 200 ml / min by the first peristaltic pump.

[0084] In one embodiment, the washing function pipeline 20 includes a second single connector 201, a third connecting pipe 202, a second reducing connector 203, a fourth connecting pipe 204, a first equal diameter connector 205, a first peristaltic pump pipe 206, a second equal diameter connector 207, a first thermoplastic pipe 208, a second Luer connector 209, and a second Luer cap 210 connected in sequence. A second clamping member 211 is provided on the outside of the third connecting pipe 202. The second single connector 201 is connected to the inlet and outlet ports 2.

[0085] like Figure 1 As shown, the second single connector 201 is a 1 / 4" single connector; the third connecting tube 202 is a 3 / 16" (4.8*8) medical silicone tube with a length of 60mm; the second reducing connector 203 is a 1 / 4" to 1 / 8" reducing connector; the fourth connecting tube 204 is a 1 / 8" (3.2*6.4) medical silicone tube with a length of 500mm; the first equal diameter connector 205 is a 1 / 8" equal diameter connector; and the first peristaltic pump tube 206 is a 1 / 8" (3.2*6.4) peristaltic pump tube with a length of 500mm.

[0086] The second equal diameter connector 207 is a 1 / 8" equal diameter connector, the first thermoplastic tube 208 is a 1 / 8" (1 / 8*1 / 4) thermoplastic tube with a length of 500mm, the second Luer connector 209 is a 1 / 8" female Luer connector, and the second Luer cap 210 is a female Luer cap.

[0087] The second Luer connector 209 can be directly connected to various commercially available disposable liquid bag interfaces for liquid transfer. Figure 1These liquid bags are collectively referred to as the second self-sealing bag 212. The second self-sealing bag 212 can be a waste liquid storage device, a resuspension liquid supply device, or other functional components, depending on the requirements. When the washing function pipeline 20 is not connected to the second self-sealing bag 212, the second Luer cap 210 is connected to the second Luer connector 209 to protect the washing function pipeline 20 from contamination. When it is necessary to connect it to the second self-sealing bag 212, the second Luer cap 210 is first removed, and then the second Luer connector 209 is connected to the second self-sealing bag 212. The above components can be connected by cable ties 400, or they can be nested with adjacent components based on their shape.

[0088] A second clamping member 211 is provided outside the third connecting tube 202. The second clamping member 211 can be a 1 / 4" Y-shaped clamp. When liquid needs to be introduced, the third connecting tube 202 can be adjusted to be located in the wide slot of the second clamping member 211 to keep the liquid inside the third connecting tube 202 flowing relatively smoothly. When liquid does not need to be introduced or the liquid introduction speed needs to be slowed down, the third connecting tube 202 can be adjusted to be stuck in the narrow slot of the second clamping member 211, so that the liquid inside the third connecting tube 202 flows slowly or stops flowing.

[0089] In one embodiment, the first peristaltic pump tube 206 is connected to the second peristaltic pump. After the second peristaltic pump is connected to the first peristaltic pump tube 206, the waste liquid can be controlled to automatically flow out of the inlet / outlet 2, and the resuspended liquid can be automatically flow into the inlet / outlet 2. The liquid flow rate can be adjusted to any value between 0 and 200 ml / min by the second peristaltic pump.

[0090] In one embodiment, the liquid outlet pipeline 30 includes a third single connector 301, a fifth connecting pipe 302, a third reducing connector 303, a sixth connecting pipe 304, a third equal diameter connector 305, a second peristaltic pump pipe 306, a fourth equal diameter connector 307, a second thermoplastic pipe 308, a third Luer connector 309, and a third Luer cap 310 connected in sequence. A third clamping member 311 is provided on the outside of the fifth connecting pipe 302. The third single connector 301 is connected to the liquid outlet 3.

[0091] like Figure 1As shown, the third single connector 301 is a 1 / 4" single connector, the fifth connecting tube 302 is a 3 / 16" (4.8*8) medical silicone tube with a length of 60mm, the third reducing connector 303 is a 1 / 4" to 1 / 8" reducing connector, the sixth connecting tube 304 is a 1 / 8" (3.2*6.4) medical silicone tube with a length of 500mm, the third equal diameter connector 305 is a 1 / 8" equal diameter connector, the second peristaltic pump tube 306 is a 1 / 8" (3.2*6.4) peristaltic pump tube with a length of 500mm, the fourth equal diameter connector 307 is a 1 / 8" equal diameter connector, the second thermoplastic tube 308 is a 1 / 8" (1 / 8*1 / 4) thermoplastic tube with a length of 500mm, the third Luer connector 309 is a 1 / 8" male Luer connector, and the third Luer cap 310 is a male Luer cap.

[0092] The third Luer connector 309 can be connected to the third self-sealing bag 312, which can be a cell storage device or other functional components as needed. When the outlet line 30 is not connected to the third self-sealing bag 312, the third Luer cap 310 is connected to the third Luer connector 309 to protect the outlet line 30 from contamination. When it is necessary to connect it to the third self-sealing bag 312, the third Luer cap 310 should be removed first, and then the third Luer connector 309 should be connected to the third self-sealing bag 312. The above components can be connected by cable ties 400 or by fitting together with adjacent components based on their shape.

[0093] A third clamping member 311 is provided outside the fifth connecting tube 302. The third clamping member 311 can be a 1 / 4" Y-shaped clamp. When liquid needs to be introduced, the fifth connecting tube 302 can be adjusted to be located in the wide slot of the third clamping member 311 to keep the liquid inside the fifth connecting tube 302 flowing relatively smoothly. When liquid does not need to be introduced or the liquid introduction speed needs to be slowed down, the fifth connecting tube 302 can be adjusted to be stuck in the narrow slot of the third clamping member 311, so that the liquid inside the fifth connecting tube 302 flows slowly or stops flowing.

[0094] In one embodiment, the second peristaltic pump tube 306 is connected to the third peristaltic pump. After the third peristaltic pump is connected to the second peristaltic pump tube 306, cells can be controlled to automatically flow out from the outlet 3. The flow rate of the liquid can be adjusted to any value between 0 and 200 ml / min by the third peristaltic pump.

[0095] In one embodiment, the outlet line 30 is connected to a cell filling device. The third Luer connector 309 of the outlet line 30, in addition to connecting to the third self-sealing bag 312, can also be directly connected to the cell filling device. After washing, the cell suspension transferred to the cell filling device does not need to be transferred again and can be directly used for subsequent clinical treatment, which is more conducive to ensuring the sterility of the product.

[0096] It should be noted that in the above embodiments, the first, second, and third peristaltic pumps can be the same peristaltic pump or different peristaltic pumps. Those skilled in the art can choose to connect the three pipelines to different peristaltic pumps or to the same peristaltic pump as needed. Both methods can achieve automatic liquid inlet and outlet, as well as speed regulation during the liquid inlet and outlet process. Furthermore, the models and specifications of the connecting pipes and connectors in each pipeline can also be adaptively adjusted as needed, as long as the liquid inlet and outlet function is achieved.

[0097] The embodiments of this utility model have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of this utility model. The description of the above embodiments is only for the purpose of helping to understand the method and core ideas of this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of this utility model. Therefore, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. A cell resuscitation fluid washing device, characterized in that, The device includes a cell resuscitation fluid washing bag, an inlet pipe, a washing function pipe, and an outlet pipe. The cell resuscitation fluid washing bag has a first side and a second side arranged opposite to each other. The first side is provided with an inlet and an outlet, and the second side is provided with an inlet and an outlet. A retaining membrane is provided between the first side and the inlet and outlet. The inlet is connected to the inlet pipe, the outlet is connected to the outlet pipe, and the inlet and outlet are connected to the washing function pipe.

2. The cell resuscitation fluid washing device as described in claim 1, characterized in that, The cell resuscitation fluid washing bag also includes a plastic filter screen, which is disposed between the first side and the inlet / outlet, and the retention membrane is adhered to the plastic filter screen.

3. The cell resuscitation fluid washing device as described in claim 1, characterized in that, The second orthographic projection of the inlet / outlet on the retention membrane is located between the first orthographic projection of the inlet on the retention membrane and the third orthographic projection of the outlet on the retention membrane.

4. The cell resuscitation fluid washing device as described in claim 1, characterized in that, The liquid inlet pipeline includes a first single connector, a first connecting pipe, a first reducing connector, a second connecting pipe, a first Luer connector, and a first Luer cap connected in sequence. A first clamping member is provided outside the first connecting pipe, and the first single connector is connected to the liquid inlet.

5. The cell resuscitation fluid washing device as described in claim 4, characterized in that, The second connecting pipe is connected to the first peristaltic pump.

6. The cell resuscitation fluid washing device as described in claim 1, characterized in that, The washing function piping includes a second single connector, a third connecting pipe, a second reducing connector, a fourth connecting pipe, a first equal diameter connector, a first peristaltic pump pipe, a second equal diameter connector, a first thermoplastic pipe, a second Luer connector, and a second Luer cap, which are connected in sequence. A second clamping member is provided on the outside of the third connecting pipe. The second single connector is connected to the inlet and outlet.

7. The cell resuscitation fluid washing device as described in claim 6, characterized in that, The first peristaltic pump tube is connected to the second peristaltic pump.

8. The cell resuscitation fluid washing device as described in claim 1, characterized in that, The liquid outlet pipeline includes a third single connector, a fifth connecting pipe, a third reducing connector, a sixth connecting pipe, a third equal diameter connector, a second peristaltic pump pipe, a fourth equal diameter connector, a second thermoplastic pipe, a third Luer connector, and a third Luer cap, which are connected in sequence. A third clamping member is provided on the outside of the fifth connecting pipe. The third single connector is connected to the liquid outlet.

9. The cell resuscitation fluid washing device as described in claim 8, characterized in that, The second peristaltic pump tube is connected to the third peristaltic pump.

10. The cell resuscitation fluid washing device as described in claim 1, characterized in that, The liquid outlet line is connected to the cell filling device.

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