A sampling device for protein renaturation reactions

Abstract

The utility model discloses a kind of sampling devices for protein renaturation reaction, including sampling test tube, fixed support plate, limit frame, motor, limit threaded column, limit plate, sampling part and rotary adjusting disc, the fixed support plate is evenly fixed and installed in the bottom outside side end of the limit frame.This utility model can carry out continuous stable equal sampling, in the sampling process, electromagnetic valve and second electromagnetic valve are opened synchronously, while high-pressure gas is transported to the inside of filling cylinder simultaneously by connecting pipe, so that sampling liquid is sprayed in rotary nozzle, motor is started intermittently, rotary adjusting disc is rotated, rotary nozzle passes above each sampling test tube in turn during rotation, and electromagnetic valve and second electromagnetic valve are opened synchronously for the same time every time, so that the amount of sampling liquid added in each sampling test tube is guaranteed to be the same.

Description

Technical Field

[0001] This utility model relates to the field of biopharmaceutical technology, specifically to a sampling device for protein refolding reactions. Background Technology

[0002] Protein refolding is a crucial step in biopharmaceutical and biotechnology research. During protein expression, insoluble inclusion bodies often form. Proteins within these inclusion bodies require refolding to restore their native conformation and biological activity. Protein refolding reactions are typically carried out in specific reaction systems, such as solutions containing denaturants, removal agents, and buffers. During protein refolding, periodic sampling and analysis of the reaction system are necessary to monitor reaction progress, assess refolding effectiveness, and optimize reaction conditions. However, existing sampling methods have several drawbacks: they cannot provide continuous, stable, and convenient equal-volume sampling; the sampling process is cumbersome and inconvenient; and they hinder precise monitoring of the reaction progress. Therefore, a device is needed to address these issues. Utility Model Content

[0003] The purpose of this invention is to provide a sampling device for protein refolding reactions, so as to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a sampling device for protein refolding reactions, comprising a sampling tube, a fixed support plate, a limiting frame, a motor, a limiting threaded column, a limiting plate, a sampling part, and a rotating adjustment disk. The fixed support plate is uniformly fixedly installed on the bottom outer end of the limiting frame. The rotating adjustment disk is rotatably engaged in the middle of the limiting frame. The motor is fixedly installed on the bottom end of the limiting frame via a reducer, and the drive end of the motor is fixedly connected to the middle of the bottom end of the rotating adjustment disk. The sampling part is located in the middle of the upper end of the rotating adjustment disk. The limiting plate is uniformly fixedly installed on the outer end of the limiting frame. The sampling tube is inserted into the inside of the limiting plate. The limiting threaded column is threadedly rotatably installed in the middle of the limiting plate, and the front end of the limiting threaded column contacts the sampling tube.

[0005] Preferably, a supporting limiting plate is fixedly installed at the bottom of the outer end of the limiting frame, and the supporting limiting plate is located directly below the limiting plate, with the bottom end of the sampling tube located on the supporting limiting plate.

[0006] Preferably, the sampling unit includes a connecting pipe, a solenoid valve, a rotating connecting seat, a limiting side frame, a connecting plate, a rotating nozzle, a delivery pipe, a second solenoid valve, a clamping plate, a filling cylinder, and an adding nozzle. The limiting side frame is fixedly installed in the middle of the connecting plate. The clamping plate is evenly fixedly installed on the limiting side frame by bolts, and the filling cylinder is clamped between the clamping plate and the limiting side frame. The adding nozzle is located on the outer side of the upper end of the filling cylinder. The rotating connecting seat is rotatably clamped to the middle of the upper end of the filling cylinder. The solenoid valve is fixedly installed in the middle of the upper end of the rotating connecting seat. The connecting pipe is fixedly installed in the middle of the upper end of the solenoid valve. The second solenoid valve is fixedly connected to the bottom side of the filling cylinder. The delivery pipe is fixedly connected to the second solenoid valve. The rotating nozzle is rotatably clamped to the bottom of the delivery pipe opposite to the second solenoid valve.

[0007] Preferably, the connecting plate is fixedly installed in the middle of the rotating adjusting plate by bolts.

[0008] Preferably, when the delivery tube swings in both directions, the rotating nozzle passes directly above each sampling tube.

[0009] Preferably, the filling cylinder is made of transparent glass.

[0010] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0011] This invention enables continuous, stable, and equal-volume sampling. During sampling, the solenoid valve and the second solenoid valve are opened simultaneously, and high-pressure gas is supplied to the filling cylinder through the connecting pipe, causing the sampling liquid to be sprayed out of the rotating nozzle. The motor drives the rotating adjustment disc to rotate intermittently. During the rotation, the rotating nozzle passes over each sampling tube in sequence, and the solenoid valve and the second solenoid valve open synchronously for the same time each time, ensuring that the amount of sampling liquid added to each sampling tube is the same. The rotating nozzle can be rotated at the bottom of the delivery pipe to adjust the sampling angle, allowing the device to adapt to sampling needs at different positions and angles, greatly improving the stability and accuracy of sampling. It can quickly complete the sampling of multiple sampling tubes at one time, enabling multiple sets of experiments to be carried out simultaneously. In the protein refolding reaction, multiple sets of experiments can be used to more comprehensively monitor the reaction process and obtain richer data information. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the main three-dimensional structure of the present utility model;

[0013] Figure 2 This is a schematic diagram of the outer structure of the limiting frame in this utility model;

[0014] Figure 3 This is a schematic diagram of the sampling section structure in this utility model.

[0015] In the diagram: 1-Sampling tube, 2-Fixed support plate, 3-Limiting frame, 4-Motor, 5-Supporting limiting plate, 6-Limiting threaded column, 7-Limiting plate, 8-Sampling part, 9-Rotating adjusting plate, 10-Connecting pipe, 11-Solenoid valve, 12-Rotating connecting seat, 13-Limiting side frame, 14-Connecting plate, 15-Rotating nozzle, 16-Transmitting pipe, 17-Second solenoid valve, 18-Clamping plate, 19-Adding cylinder, 20-Adding nozzle. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] Please see Figure 1-3 This utility model provides an embodiment of a sampling device for protein refolding reactions, comprising a sampling tube 1, a fixed support plate 2, a limiting frame 3, a motor 4, a limiting threaded post 6, a limiting plate 7, a sampling part 8, and a rotating adjustment disk 9. The fixed support plate 2 is uniformly fixedly installed on the bottom outer end of the limiting frame 3. The rotating adjustment disk 9 is rotatably engaged in the middle of the limiting frame 3. The motor 4 is fixedly installed on the bottom end of the limiting frame 3 through a reducer, and the driving end of the motor 4 is fixedly connected to the middle of the bottom end of the rotating adjustment disk 9. The sampling part 8 is located in the middle of the upper end of the rotating adjustment disk 9. The limiting plate 7 is uniformly fixedly installed on the outer end of the limiting frame 3. The sampling tube 1 is inserted into the inside of the limiting plate 7. The limiting threaded post 6 is threadedly rotatably installed in the middle of the limiting plate 7, and the front end of the limiting threaded post 6 is in contact with the sampling tube 1.

[0018] A support limiting plate 5 is fixedly installed at the bottom of the outer end of the limiting frame 3, and the support limiting plate 5 is located directly below the limiting plate 7. The bottom end of the sampling tube 1 is located on the support limiting plate 5.

[0019] The sampling unit 8 includes a connecting pipe 10, a solenoid valve 11, a rotating connecting seat 12, a limiting side frame 13, a connecting plate 14, a rotating nozzle 15, a delivery pipe 16, a second solenoid valve 17, a clamping plate 18, a filling cylinder 19, and a filling nozzle 20. The limiting side frame 13 is fixedly installed in the middle of the connecting plate 14. The clamping plate 18 is evenly fixedly installed on the limiting side frame 13 by bolts, and the filling cylinder 19 is secured between the clamping plate 18 and the limiting side frame 13. The filling nozzle 20... The upper outer side of the filling cylinder 19 is provided with a rotating connecting seat 12 that is rotatably engaged with the middle of the upper end of the filling cylinder 19. The solenoid valve 11 is fixedly installed in the middle of the upper end of the rotating connecting seat 12. The connecting pipe 10 is fixedly installed in the middle of the upper end of the solenoid valve 11. The second solenoid valve 17 is fixedly connected to the bottom side of the filling cylinder 19. The delivery pipe 16 is fixedly connected to the second solenoid valve 17. The rotating nozzle 15 is rotatably engaged with the bottom of the end of the delivery pipe 16 that is away from the second solenoid valve 17.

[0020] The connecting plate 14 is fixedly installed in the middle of the rotating adjusting plate 9 by bolts, which facilitates disassembly and use.

[0021] When the delivery tube 16 swings in both directions, the rotating nozzle 15 passes directly above each sampling tube 1, which allows each sampling tube 1 to continuously take equal amounts of samples.

[0022] The filling cylinder 19 is made of transparent glass, allowing observation of the amount of remaining liquid.

[0023] Working principle: The sampling tube 1 is evenly placed inside the limiting plate 7. The bottom of the sampling tube 1 can be supported and limited by the supporting limiting plate 5. When the diameter of the sampling tube 1 is small, the limiting threaded post 6 can be screwed to achieve auxiliary vertical fixation. It is connected to external equipment such as air pump through the connecting pipe 10 and the hose. The sampling liquid can be added into the filling cylinder 19 by screwing down the adding nozzle 20. The filling cylinder 19 is clamped and fixed between the limiting side frame 13 and the clamping plate 18 by the threaded clamping plate 18. During the sampling process, the solenoid valve 11 and the second solenoid valve 17 are opened simultaneously, and high pressure is delivered into the filling cylinder 19 through the connecting pipe 10. The gas causes the sampling liquid to be sprayed out of the rotating nozzle 15. Then, the solenoid valve 11 and the second solenoid valve 17 are closed. The motor 4 is started, which can drive the rotating adjustment disk 9 to rotate intermittently. During the rotation, the rotating nozzle 15 can pass over the sampling tube 1 in sequence. When it passes, the solenoid valve 11 and the second solenoid valve 17 open synchronously for the same time, so that the same amount of sampling liquid can be added to each sampling tube 1. The rotating nozzle 15 can rotate at the bottom of the delivery tube 16 to adjust the sampling angle, so that each sampling tube 1 can be sampled quickly at one time. This allows for multiple sets of experiments to be conducted with the same sampling, which is convenient for monitoring the reaction process, evaluating the refolding effect, and optimizing the reaction conditions.

[0024] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A sampling device for protein refolding reactions, comprising a sampling tube (1), a fixed support plate (2), a limiting frame (3), a motor (4), a limiting threaded column (6), a limiting plate (7), a sampling part (8), and a rotating adjustment disk (9), characterized in that: The fixed support plate (2) is uniformly fixedly installed on the bottom outer end of the limiting frame (3). The rotating adjustment disk (9) is rotatably engaged in the middle of the limiting frame (3). The motor (4) is fixedly installed at the bottom end of the limiting frame (3) through a reducer, and the driving end of the motor (4) is fixedly connected to the middle of the bottom end of the rotating adjustment disk (9). The sampling part (8) is set in the middle of the upper end of the rotating adjustment disk (9). The limiting plate (7) is uniformly fixedly installed on the outer end of the limiting frame (3). The sampling test tube (1) is inserted into the inside of the limiting plate (7). The limiting threaded column (6) is rotatably installed in the middle of the limiting plate (7), and the front end of the limiting threaded column (6) is in contact with the sampling test tube (1).

2. The sampling device for protein refolding reaction according to claim 1, characterized in that: The bottom of the outer end of the limiting frame (3) is fixedly installed with a supporting limiting plate (5), and the supporting limiting plate (5) is located directly below the limiting plate (7). The bottom end of the sampling tube (1) is located on the supporting limiting plate (5).

3. A sampling device for protein refolding reaction according to claim 2, characterized in that: The sampling unit (8) includes a connecting pipe (10), a solenoid valve (11), a rotating connecting seat (12), a limiting side frame (13), a connecting plate (14), a rotating nozzle (15), a delivery pipe (16), a second solenoid valve (17), a clamping plate (18), a filling cylinder (19), and a filling nozzle (20). The limiting side frame (13) is fixedly installed in the middle of the connecting plate (14). The clamping plate (18) is evenly fixedly installed on the limiting side frame (13) by bolts, and the filling cylinder (19) is secured between the clamping plate (18) and the limiting side frame (13). The filling nozzle (20) The upper outer side of the filling cylinder (19) is provided, the rotating connecting seat (12) is rotatably clamped to the middle of the upper end of the filling cylinder (19), the solenoid valve (11) is fixedly installed in the middle of the upper end of the rotating connecting seat (12), the connecting pipe (10) is fixedly installed in the middle of the upper end of the solenoid valve (11), the second solenoid valve (17) is fixedly connected to the bottom side of the filling cylinder (19), the delivery pipe (16) is fixedly connected to the second solenoid valve (17), and the rotating nozzle (15) is rotatably clamped to the bottom of the end of the delivery pipe (16) away from the second solenoid valve (17).

4. A sampling device for protein refolding reaction according to claim 3, characterized in that: The connecting plate (14) is fixedly installed in the middle of the rotating adjusting plate (9) by bolts.

5. A sampling device for protein refolding reaction according to claim 4, characterized in that: When the delivery tube (16) swings forward and backward, the rotating nozzle (15) passes directly above each sampling tube (1).

6. A sampling device for protein refolding reaction according to claim 5, characterized in that: The filling cylinder (19) is made of transparent glass.

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