CELL CULTURE PROCEDURES
Patent Information
- Application Number
- DE602015090655
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2014-03-19
- Filing Date
- 2015-03-17
- Publication Date
- 2024-12-18
- Estimated Expiration
- 2035-03-17
AI Technical Summary
Mammalian cell cultures face growth inhibition due to the accumulation of metabolites such as 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenylpyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, and isovalerate, which limit maximum cell density and productivity in protein production processes.
Maintaining these metabolites at low concentrations in the cell culture medium by measuring their levels and adjusting the precursor supply to prevent accumulation above a predefined threshold, specifically below 3mM, in a fed-batch culture system.
This approach enhances cell growth and productivity by preventing metabolic inhibition, allowing for increased viable cell density and protein titers, thereby improving the efficiency of recombinant protein production.