ITACONIC ACID DERIVATIVES AND THEIR USE FOR THE TREATMENT OF AN INFLAMMATORY DISEASE OR A DISEASE ASSOCIATED WITH AN ADVERSE IMMUNE RESPONSE

Description

Field of the invention

[0001] The present invention relates to compounds and their use in treating or preventing inflammatory diseases or diseases associated with an undesirable immune response, and to related compositions and intermediate compounds.Background of the invention

[0002] Chronic inflammatory diseases such as rheumatoid arthritis, systemic lupus erythematosus (SLE), multiple sclerosis, psoriasis, Crohn's disease, ulcerative colitis, uveitis and chronic obstructive pulmonary disease (COPD) represent a significant burden to society because of lifelong debilitating illness, increased mortality and high costs for therapy and care (Straub R.H. and Schradin C., 2016). Non-steroidal anti-inflammatory drugs (NSAIDs) are the most widespread medicines employed for treating inflammatory disorders, but these agents do not prevent the progression of the inflammation and only treat the accompanying symptoms. Glucocorticoids are powerful anti-inflammatory agents, making them emergency treatments for acute inflammatory flares, but given longer term these medicines give rise to a plethora of unwanted side-effects and may also be subject to resistance (Straub R. H. and Cutolo M., 2016). Thus, considerable unmet medical need still exists for the treatment of inflammatory disorders and extensive efforts to discover new medicines to alleviate the burden of these diseases is ongoing (Hanke T. et al., 2016).

[0003] Dimethyl fumarate (DMF), a diester of the citric acid cycle (CAC) intermediate fumaric acid, is utilised as an oral therapy for treating psoriasis (Brück J. et al., 2018) and multiple sclerosis (Mills E. A. et al., 2018). Importantly, following oral administration, none of this agent is detected in plasma (Dibbert S. et al., 2013), the only drug-related compounds observed being the hydrolysis product monomethyl fumarate (MMF) and glutathione (GSH) conjugates of both the parent (DMF) and metabolite (MMF). DMF's mechanism of action is complex and controversial. This compound's efficacy has been attributed to a multiplicity of different phenomena involving covalent modification of proteins and the conversion of "prodrug" DMF to MMF. In particular, the following pathways have been highlighted as being of relevance to DMF's anti-inflammatory effects: 1) activation of the anti-oxidant, anti-inflammatory, nuclear factor (erythroid-derived 2)-like 2 (NRF2) pathway as a consequence of reaction of the electrophilic α,β-unsaturated ester moiety with nucleophilic cysteine residues on kelch-like ECH-associated protein 1 (KEAP1) (Brennan M. S. et al., 2015); 2) induction of activating transcription factor 3 (ATF3), leading to suppression of pro-inflammatory cytokines interleukin (IL)-6 and IL-8 (Müller S. et al., 2017); 3) inactivation of the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) through succination of its catalytic cysteine residue with a Michael accepting unsaturated ester (Kornberg M. D. et al., 2018; Angiari S. and O'Neill L. A., 2018); 4) inhibition of nuclear factor-kappaB (NF-κB)-driven cytokine production (Gillard G. O. et al., 2015); 5) preventing the association of PKCB with the costimulatory receptor CD28 to reduce the production of IL-2 and block T-cell activation (Blewett M. M. et al., 2016); 6) reaction of the electrophilic α,β-unsaturated ester with the nucleophilic thiol group of anti-oxidant GSH, impacting cellular responses to oxidative stress (Lehmann J. C. U. et al., 2007); 7) agonism of the hydroxycarboxylic acid receptor 2 (HCA2) by the MMF generated in vivo through DMF hydrolysis (von Glehn F. et al., 2018); 8) allosteric covalent inhibition of the p90 ribosomal S6 kinases (Andersen J. L. et al., 2018); 9) inhibition of the expression and function of hypoxia-inducible factor-1α (HIF-1α) and its target genes, such as IL-8 (Zhao G. et al., 2014); and 10) inhibition of Toll-like receptor (TLR)-induced M1 and K63 ubiquitin chain formation (McGuire V. A. et al., 2016). In general, with the exception of HCA2 agonism (Tang H. et al., 2008), membrane permeable diester DMF tends to exhibit much more profound biological effects in cells compared to its monoester counterpart MMF. However, the lack of systemic exposure of DMF in vivo has led some researchers to assert that MMF is, in fact, the principal active component following oral DMF administration (Mrowietz U. et al., 2018). As such, it is evident that some of the profound biology exerted by DMF in cells is lost because of hydrolysis in vivo to MMF.

[0004] Recently, it has been discovered that, during inflammatory macrophage activation, the CAC becomes anaplerotic and is diverted such that the unsaturated diacid itaconic acid, "itaconate", is generated (Murphy M. P. and O'Neill L. A. J., 2018; O'Neill L. A. J. and Artyomov M. N., 2019; Yu X.-H. et al., 2019). Instead of being hydrated to isocitrate by aconitate hydratase, the CAC intermediate aconitate is decarboxylated by the protein product of immune-responsive gene 1 (IRG1), one of the most highly upregulated genes in macrophages under proinflammatory conditions, subsequently named aconitate decarboxylase 1, to produce itaconic acid (Michelucci A. et al., 2013). This unsaturated diacid is an inhibitor of the bacterial enzyme isocitrate lyase and, as such, it exerts anti-bacterial activity. In addition, itaconic acid has been shown to inhibit the CAC enzyme succinate dehydrogenase (SDH) (Ackermann et al., 1949), leading accordingly to succinate accumulation (Cordes T. et al., 2016). By inhibiting SDH, an enzyme critical for the inflammatory response (E. L. Mills et al., 2016), itaconate ameliorates inflammation in vitro and in vivo during macrophage activation and ischemia-reperfusion injury (Lampropoulou V. et al., 2016).

[0005] Like fumaric acid, itaconic acid is an α,β-unsaturated carboxylic acid. As such, it is a Michael acceptor which induces a global electrophilic stress response. In this regard, the itaconic acid diester dimethyl itaconate (DMI), like DMF, produces an anti-inflammatory response, reducing the expression levels of pro-inflammatory cytokines IL-1β, IL-6, IL-12 and IL-18 in lipopolysaccharide (LPS)-stimulated bone marrow-derived macrophages (WO2017 / 142855A1). This response appears to be mediated, in part, by NRF2 activation, via alkylation of KEAP1 cysteine residues by the electrophilic α,β-unsaturated ester moiety (Mills E. L. et al., 2018), which enhances the expression of downstream genes with anti-oxidant and anti-inflammatory capacities. Nevertheless, not all of the pronounced immunoregulatory effects engendered by DMI can be attributed to NRF2 activation. In particular, the modulation of IκBζ by DMI is independent of NRF2 and is mediated via upregulation of ATF3, a global negative regulator of immune activation that downregulates various cytokines, such as IL-6 (Bambouskova M. et al., 2018). Moreover, by inhibiting IκBζ protein production, DMI ameliorates IL-17-mediated pathologies, highlighting the therapeutic potential of this regulatory pathway (WO2019 / 036509A1). Further highlighting its pharmacologic potential, DMI has recently been reported to 1) demonstrate a protective effect on cerebral ischemia / reperfusion injury, thereby offering potential for the treatment of ischemic stroke (Zhang D. et al., 2019); 2) provide protection from the cardiotoxic effects of doxorubicin (Shan Q. et al., 2019); and 3) protect against lippolysacchride-induced mastitis in mice by activating MAPKs and NRFrf2 while inhibiting NF-κB signaling pathways (Zhao C. et al., 2019). Furthermore, DMI is said to have utility in preventing and treating ulcerative colitis and canceration thereof (CN110731955, Sun Yat-sen University Cancer Center); and has been reported to protect against fungal keratitis by activating the NRF2 / HO-1 signalling pathway (Gu L. et al., 2020). Nevertheless, it should be noted that DMI is not metabolised to itaconic acid intracellularly (EIAzzouny M. et al., 2017). Other α,β-unsaturated esters exhibit IL-1β-lowering effects in macrophages by inhibiting the NLRP3 inflammasome (Cocco M. et al., 2017 and 2014), and have been demonstrated to inhibit the TLR4 pathway, leading ultimately to suppression of LPS-induced stimulation of NF-κB, tumour necrosis factor (TNF)-α, IL-1β and nitric oxide release (Zhang S. et al., 2012).

[0006] Other itaconic acid derivatives have been demonstrated to elicit anti-inflammatory effects (Bagavant G. et al., 1994). A notable example is 4-octyl itaconic acid (4OI), an itaconate derivative with improved cellular uptake. Since the α,β-unsaturated carboxylic acid is not esterified in 4OI, this electrophile exhibits low reactivity with biological thiols (Schmidt T. J. et al., 2007), much like the situation encountered with itaconic acid itself. As a result of its low reactivity / electrophilicity, the NRF2-activating effects of 4OI are not attenuated by GSH, in contrast to the findings with the much more reactive DMI. In this latter case, the α,β-unsaturated carboxylic acid is esterified and, as a consequence, the IL-6-lowering and NRF2-activating effects of DMI are reversed by the thiols N-acetylcysteine and GSH, respectively. Through the reaction with KEAP1 and the resulting NRF2 activation, as well as GAPDH inhibition (Liao S.-T. et al., 2019), 4OI has been demonstrated to produce a wide range of interesting biological effects, including: 1) protection of neuronal cells from hydrogen peroxide (Liu H. et al., 2018); 2) inhibition of proinflammatory cytokine production in peripheral blood mononuclear cells of SLE patients (Tang C. et al., 2018); and 3) protection of human umbilical vein endothelial cells from high glucose (Tang C. et al., 2019); 4) inhibition of osteoclastogenesis by suppressing the E3 ubiquitin ligase Hrd1 and activating NRF2 signaling (Sun X. et al., 2019); 5) induction of repression of STING by NRF2 and type I IFN production in cells from patients with STING-dependent interferonopathies (Olagnier D. et al., 2018); 6) protection against renal fibrosis via inhibiting the TGF-beta / Smad pathway, autophagy and reducing generation of reactive oxygen species (Tian F. et al., 2020); 7) reduction of brain viral burden in mice intracranially injected with Zika virus (Daniels B. P. et al. 2019); and 8) protection against liver ischemia-reperfusion injury (Yi F. et al. 2020). Furthermore, itaconate has been reported to modulate tricarboxylic acid and redox metabolism to mitigate reperfusion injury (Cordes T. et al., 2020). In addition, raised plasma itaconate levels demonstrate a clear correlation with reduction in rheumatoid arthritis disease activity scores following commencement of therapy with conventional disease modifying anti-rheumatic drug (cDMARD) therapy (Daly R. et al. 2019).

[0007] Artyomov et al. (WO2017 / 142855; WO2019 / 036509) disclose the use of itaconate, malonate or a derivative thereof as an immunomodulatory agent.

[0008] Funcke et al. (Arzneimittel-Forschung, 1954, vol. 4, no. 8, 492-494) discloses the influence of structural changes in nitrogen-free esters with spasmolytic effect.

[0009] US2012 / 270155 (Yoshitaka et al.) discloses compounds which are said to be useful for acid generation in a resist composition and related aspects.

[0010] Dominguez et al. (Monatshefte Für Chemie, vol. 120, 1989, 743-748) discloses a series of mono and diesters of itaconate acid.

[0011] Pan et al. (Polymer Preprints, vol. 43, no. 2, 2002, 620-621) discloses hydrogels composed of itaconates and acrylates.

[0012] In spite of the above findings, there remains a need to identify and develop new itaconate derivatives possessing enhanced properties compared to currently marketed anti-inflammatory agents, such as DMF. The present inventors have now discovered, surprisingly, that certain itaconate diesters are highly effective at reducing cytokine release in cells and / or in activating NRF2-driven effects. These properties, amongst others, make them potentially more effective than DMI and / or dimethyl fumarate. Such compounds therefore possess excellent anti-inflammatory properties.Summary of the invention

[0013] The present invention provides a compound of formula (I): wherein, R A< is selected from the group consisting of C 1-10 alkyl, C 3-10 cycloalkyl and C 5-10 spirocycloalkyl; wherein R A< is not substituted or is substituted by one or more substituents selected from the group consisting of oxo, R 1A< , OR 2A< , NR 2A< R 3A< , SR 2A< , SOR 9A< , SO 2 R 9A< , SO 2 NR 2A< R 3A< , C(O)R 2A< and CONR 2A< R 3A< ; R 1A< is selected from the group consisting of fluoro, methyl, cyano, SiR 4A< R 5A< R 6A< , C 3-8 cycloalkyl and phenyl; wherein methyl, C 3-8 cycloalkyl and phenyl are not substituted or are substituted by R 7A< and / or R 8A< ; R 4A< , R 5A< and R 6A< are independently selected from the group consisting of C 1-4 alkyl and phenyl; R 7A< and R 8A< are independently selected from the group consisting of oxo, C 1-4 alkyl, C 1-4 alkoxy, C 1-4 haloalkyl, C 1-4 haloalkoxy, hydroxy, CO 2 H, cyano, methanesulfonyl and halo; or, taken together, R 7A< and R 8A< form a C 3-8 cycloalkyl or 4-7 membered heterocyclic ring; R 2A< and R 3A< are independently H, C 1-8 alkyl, C 3-8 cycloalkyl or phenyl; or, taken together, R 2A< and R 3A< form a 4-7 membered heterocyclic ring; R 9A< is C 1-8 alkyl, C 3-8 cycloalkyl or phenyl; and R B< is C 1-2 alkyl substituted by CO 2 H and is optionally further substituted by trifluoromethyl or methyl; R C< and R D< are independently selected from the group consisting of H, C 1-2 alkyl, hydroxy, methoxy and fluoro; or a pharmaceutically acceptable salt and / or solvate thereof.

[0014] The present invention provides a pharmaceutical composition comprising a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof.

[0015] The present invention provides a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof for use as a medicament.

[0016] The present invention provides a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof for use in treating or preventing an inflammatory disease or a disease associated with an undesirable immune response.

[0017] Also provided are intermediate compounds of use in the preparation of compounds of formula (I).Detailed description of the invention Compounds of formula (I)

[0018] Embodiments and preferences set out herein with respect to the compound of formula (I) apply equally to the pharmaceutical composition and compound for use aspects of the invention.

[0019] The term "C 1-10 alkyl" refers to a straight or branched fully saturated hydrocarbon group having from 1 to 10 carbon atoms. The term encompasses methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, n-heptyl, n-hexyl and n-octyl. Other branched variants such as heptyl-CH(CH 3 )- and hexyl-CH(CH 3 )- are also included. Further branched variants include n-pentyl-CH(CH 2 CH 3 )- and (n-Bu) 2 CH-. Other branched variants include n-pentyl-C(CH 3 ) 2 - or n-hexyl-C(CH 3 ) 2 -. Another branched variant is -CH(t-Bu) 2 . Other alkyl groups, for example C 1-9 alkyl, C 1-8 alkyl, C 1-7 alkyl, C 1-6 alkyl, C 1-S alkyl, C 1-4 alkyl, C 1-3 alkyl, C 1-2 alkyl, C 2-10 alkyl, C 2-9 alkyl, C 2-8 alkyl, C 2-7 alkyl, C 2-6 alkyl, C 2-5 alkyl, C 2-4 alkyl, C 2-3 alkyl, C 3-10 alkyl, C 3-9 alkyl, C 3-8 alkyl, C 3-7 alkyl, C 3-6 alkyl, C 3-5 alkyl, C 3-4 alkyl, C 4-10 alkyl, C 4-9 alkyl, C 4-8 alkyl, C 4-7 alkyl, C 4-6 alkyl, C 4-5 alkyl, C 5-10 alkyl, C 5-9 alkyl, C 5-8 alkyl, C 5-7 alkyl, C 5-6 alkyl, C 6-10 alkyl, C 6-9 alkyl, C 6-8 alkyl, C 7-10 alkyl, C 7-9 alkyl, C 7-8 alkyl, C 8-10 alkyl, C 8-9 alkyl and C 9-10 alkyl are as defined above but contain different numbers of carbon atoms. The term "C 1-10 alkyl" also encompasses "C 1-10 alkylene" which is a bifunctional straight or branched fully saturated hydrocarbon group having from 1 to 10 carbon atoms. Example "C 1-10 alkylene" groups include methylene, ethylene, n-propylene, n-butylene, n-pentylene, n-heptylene, n-hexylene and n-octylene.

[0020] The term "C 1-4 alkoxy" refers to a C 1-4 alkyl group (e.g. C 1-3 alkyl group, C 1-2 alkyl group or C 1 alkyl group) as defined above, singularly bonded to oxygen. The term encompasses methoxy, ethoxy, 1-propoxy and 2-propoxy, and is suitably methoxy.

[0021] The term "C 1-4 haloalkyl" (e.g. C 1-3 haloalkyl group, C 1-2 haloalkyl group or C 1 haloalkyl group) as used herein refers to a straight or a branched fully saturated hydrocarbon chain containing the specified number of carbon atoms and at least one halogen atom, such as fluoro or chloro, especially fluoro. An example of haloalkyl is CF 3 . Further examples of haloalkyl are CHF 2 and CH 2 CF 3 .

[0022] The term "C 1-4 haloalkoxy" refers to a C 1-4 haloalkyl group (e.g. C 1-3 haloalkyl group, C 1-2 haloalkyl group or C 1 haloalkyl group) as defined above, singularly bonded to oxygen. Examples of C 1-4 haloalkoxy include OCF 3 , OCHF 2 and OCH 2 CF 3 .

[0023] The term "C 3-10 cycloalkyl" refers to a fully saturated cyclic hydrocarbon group having from 3 to 10 carbon atoms. The term encompasses cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclononyl and cyclodecyl as well as bridged systems such as bicyclo[1.1.1]pentyl, bicyclo[2.2.1]heptyl, bicyclo[2.2.2]octyl and adamantyl. Other cycloalkyl groups, for example C 3-9 cycloalkyl, C 3-8 cycloalkyl, C 3-7 cycloalkyl, C 3-6 cycloalkyl, C 3-5 cycloalkyl, C 3-4 cycloalkyl, C 4-10 cycloalkyl, C 4-9 cycloalkyl, C 4-8 cycloalkyl, C 4-7 cycloalkyl, C 4-6 cycloalkyl, C 4-5 cycloalkyl, C 5-10 cycloalkyl, C 5-9 cycloalkyl, C 5-8 cycloalkyl, C 5-7 cycloalkyl, C 5-6 cycloalkyl, C 6-10 cycloalkyl, C 6-9 cycloalkyl, C 6-8 cycloalkyl, C 6-7 cycloalkyl, C 7-10 cycloalkyl, C 7-9 cycloalkyl, C 7-8 cycloalkyl, C 8-10 cycloalkyl, C 8-9 cycloalkyl and C 0-10 cycloalkyl are as defined above but contain different numbers of carbon atoms.

[0024] The term "C 5-10 spirocycloalkyl" refers to a bicyclic cycloalkyl group wherein the two rings are connected through just one atom. The rings can be different or identical. The term encompasses spiro[3.3]heptyl. Other spirocycloalkyl groups, for example C 5-9 spirocycloalkyl, C 5-8 spirocycloalkyl and C 5-7 spirocycloalkyl are as defined above but contain different numbers of carbon atoms.

[0025] The term "4-7 membered heterocyclyl" refers to a non-aromatic cyclic group having 4 to 7 ring atoms and at least one heteroatom selected from N, O, S and B. The term "heterocyclyl" is interchangeable with "heterocyclic ring". The term encompasses oxetanyl, thietanyl, azetidinyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, tetrahydropyranyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl and homomorpholinyl. Other heterocyclyl groups, for example, 4-6 membered heterocyclyl and 4-5 membered heterocyclyl are as defined above but contain different numbers of ring atoms. 4-7 membered heterocyclyl groups can typically be substituted by one or more oxo groups. Suitably, thietanyl is substituted by one or two oxo groups. Bicyclic heterocyclic compounds are also encompassed, such as the following:

[0026] The term "hydroxy" (which may also be referred to as "hydroxyl") refers to an -OH group.

[0027] The term "oxo" refers to a =O substituent, whereby an oxygen atom is doubly bonded to carbon (e.g. C=O) or another element (e.g. S=O, S(=O) 2 ). The carbon or other element is suitably an atom of an alkyl, cycloalkyl, spirocycloalkyl or heterocyclyl group.

[0028] The term "halo" as used herein, refers to fluorine, chlorine, bromine or iodine. Particular examples of halo are fluorine and chlorine, especially fluorine.

[0029] Where substituents are indicated as being optionally substituted e.g. optionally further substituted in formula (I) in the embodiments and preferences set out below, said substituents are optionally substituted e.g. optionally further substituted as specified in the given formula unless stated otherwise, even if the possible substitution is not explicitly listed in the embodiment.

[0030] In one embodiment, R A< is selected from the group consisting of C 1-10 alkyl and C 3-10 cycloalkyl.

[0031] In one embodiment, R A< is C 1-10 alkyl, in particular C 1-8 alkyl.

[0032] Suitably, R A< is selected from the group consisting of methyl, ethyl, n-propyl, iso-propyl, n-butyl, n-pentyl, n-hexyl, n-heptyl and n-octyl, and in particular is selected from the group consisting of methyl, ethyl, n-propyl, iso-propyl, n-butyl, n-hexyl and n-octyl.

[0033] Suitably, R A< is C 6-10 alkyl, in particular n-heptyl, n-octyl, heptyl-CH(CH 3 )- and hexyl-CH(CH 3 )-, such as hexyl-CH(CH 3 )-.

[0034] Suitably, R A< is n-pentyl-C(CH 3 ) 2 - or n-hexyl-C(CH 3 ) 2 -.

[0035] Suitably, R A< is n-hexyl-CH(CH 3 )-, n-pentyl-C(CH 3 ) 2 - or n-hexyl-C(CH 3 ) 2 -.

[0036] Most suitably, R A< is hexyl-CH(CH 3 )- and has the following structure:

[0037] In one embodiment, R A< is C 3-10 cycloalkyl, in particular C 3-8 cycloalkyl. Suitably, R A< is C 6-10 cycloalkyl, in particular C 8 cycloalkyl.

[0038] Suitably, R A< is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl and bicyclo[2.2.1]heptyl; and in particular is cyclobutyl, cyclopentyl, cyclohexyl, cyclooctyl or bicyclo[2.2.1]heptyl. Suitably, R A< is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl, and in particular is cyclobutyl or cyclooctyl. Suitably, R A< is cyclooctyl. Alternatively R A< is cyclobutyl.

[0039] Suitably, R A< is 1-adamantyl:

[0040] In one embodiment, R A< is C 5-10 spirocycloalkyl, such as C 5-8 spirocycloalkyl, and in particular is spiro[3.3]heptyl.

[0041] In one embodiment, R A< e.g. as defined above, is not substituted. Suitably, R A< is hexyl-CH(CH 3 )- and is not substituted.

[0042] In another embodiment, R A< e.g. as defined above, is substituted.

[0043] In one embodiment, R A< is substituted by one or more substituents selected from the group consisting of oxo, R 1A< , NR 2A< R 3A< , SR 2A< , SOR 9A< , SO 2 R 9A< , SO 2 NR 2A< R 3A< , C(O)R 2A< and CONR 2A< R 3A< . Suitably, the one or more substituent is R 1A< . Suitably, R A< is substituted by one R 1A< . Alternatively, R A< is substituted by two R 1A< . Alternatively, R A< is substituted by three R 1A< . In any one of the above embodiments, R 1A< may be the same or different e.g. the same.

[0044] When R A< is substituted by at least one R 1A< group the substituent may replace any C-H bond present in R A< . When R A< is substituted by at least two R 1A< groups, the R 1A< groups may be on the same carbon atom or different carbon atoms. When R A< is C 3-10 cycloalkyl or C 5-10 spirocycloalkyl, the group may be substituted at the point of attachment of R A< to the oxygen atom such that the following structures form:

[0045] In one embodiment, R 1A< is selected from the group consisting of fluoro, methyl, cyano, SiR 4A< R 5A< R 6A< and phenyl.

[0046] Suitably, R 1A< is fluoro. Alternatively, R 1A< is methyl. Alternatively, R 1A< is cyano. Alternatively, R 1A< is SIR 4A< R 5A< R 6A< , Alternatively, R 1A< is C 3-8 cycloalkyl. Alternatively, R 1A< is phenyl.

[0047] Most suitably, R 1A< is selected from fluoro, methyl, C 3-8 cycloalkyl and phenyl, such as fluoro and phenyl, especially fluoro.

[0048] In another embodiment, R A< is substituted by one phenyl group. In another embodiment, R A< is substituted by three fluoro groups. Suitably the three fluoro groups are attached to the same terminal carbon atom to form a CF 3 group.

[0049] In one embodiment, R 4A< , R 3A< and R 6A< are independently selected from the group consisting of C 1-4 alkyl and phenyl. Suitably, R 4A< , R 3A< and R 6A< are independently selected from the group consisting of methyl, ethyl, isopropyl, tert-butyl and phenyl.

[0050] In one embodiment, R 1A< is substituted by R 7A< and / or R 8A< when R 1A< is methyl, C 3-8 cycloalkyl and phenyl. Suitably, R 1A< is substituted by R 7A< and R 8A< . Alternatively, R 1A< is substituted by R 7A< or R 8A< . In one embodiment, R 1A< is not substituted by R 7A< and / or R 8A< when R 1A< is methyl, C 3-8 cycloalkyl and phenyl. In one embodiment R 1A< is substituted by R 7A< and R 8A< is absent.

[0051] In one embodiment, R 7A< and R 8A< are independently selected from the group consisting of oxo, C 1-4 alkyl, C 1-4 alkoxy, hydroxy, CO 2 H, cyano, methanesulfonyl and halo; or, taken together, R 7A< and R 8A< form a C 3-8 cycloalkyl or 4-7 membered heterocyclic ring.

[0052] Suitably, R 7A< and R 8A< are independently selected from the group consisting of oxo, methyl, ethyl, methoxy, hydroxy, CO 2 H, cyano, methanesulfonyl and halo (e.g. fluoro) such as oxo, methyl, ethyl, methoxy, hydroxy, cyano, methanesulfonyl and halo (e.g. fluoro); or, taken together, R 7A< and R 8A< form a C 3-8 cycloalkyl or 4-7 membered heterocyclic ring. In one embodiment, R 7A< and R 8A< are fluoro. In one embodiment, R 7A< and R 8A< are chloro. In one embodiment, R 7A< and R 8A< are independently selected from the group consisting of oxo, C 1-4 alkyl, C 1-4 alkoxy, hydroxy, cyano, methanesulfonyl and halo.

[0053] Suitably, R 7A< is oxo. Alternatively, R 7A< is C 1-4 alkyl, such as methyl. Alternatively, R 7A< is C 1-4 alkoxy such as methoxy. Alternatively, R 7A< is C 1-4 haloalkyl such as CF 3 . Alternatively, R 7A< is C 1-4 haloalkoxy such as OCF 3 . Alternatively, R 7A< is hydroxy. Alternatively, R 7A< is CO 2 H. Alternatively, R 7A< is cyano. Alternatively, R 7A< is methanesulfonyl. Alternatively, R 7A< is halo such as chloro or fluoro, e.g., chloro. In any one of the above embodiments, suitably R 8A< is absent. Most suitably, R 7A< is halo such as chloro or fluoro, e.g., chloro, or C 1-4 haloalkyl such as CF 3 and R 8A< is absent.

[0054] Suitably, R 8A< is oxo. Alternatively, R 8A< is C 1-4 alkyl, such as methyl. Alternatively, R 8A< is C 1-4 alkoxy such as methoxy. Alternatively, R 8A< is C 1-4 haloalkyl such as CF 3 . Alternatively, R 8A< is C 1-4 haloalkoxy such as OCF 3 . Alternatively, R 8A< is hydroxy. Alternatively, R 8A< is CO 2 H. Alternatively, R 8A< is cyano. Alternatively, R 8A< is methanesulfonyl. Alternatively, R 8A< is halo such as chloro or fluoro, e.g., chloro. In any one of the above embodiments, suitably R 7A< is absent.

[0055] In another embodiment, taken together, R 7A< and R 8A< form a C 3-8 cycloalkyl or 4-7 membered heterocyclic ring. Suitably, R 7A< and R 8A< form a C 3-8 cycloalkyl ring such as a C 3-6 cycloalkyl ring. Suitably, R 7A< and R 8A< form a 4-7 membered heterocyclic ring such as a 4-6 membered heterocyclic ring.

[0056] In one embodiment, R 2A< and R 3A< are independently H, C 1-8 alkyl, C 3-8 cycloalkyl or phenyl. Suitably, R 2A< and R 3A< are independently H, C 1-4 alkyl, C 3-8 cycloalkyl or phenyl.

[0057] Suitably, R 2A< is H. Alternatively, R 2A< is C 1-8 alkyl, such as C 1-4 alkyl e.g. methyl. Alternatively, R 2A< is C 3-8 cycloalkyl such as C 3-6 cycloalkyl. Alternatively, R 2A< is phenyl.

[0058] In one embodiment, R 2A< is methyl or phenyl, in particular phenyl.

[0059] Suitably, R 3A< is H. Alternatively, R 3A< is C 1-8 alkyl, such as C 1-4 alkyl e.g. methyl. Alternatively, R 3A< is C 3-8 cycloalkyl, such as C 3-6 cycloalkyl. Alternatively, R 3A< is phenyl.

[0060] In one embodiment, R 3A< is methyl.

[0061] In one embodiment, R 2A< and R 3A< form a 4-7 membered heterocyclic ring.

[0062] In one embodiment, R 9A< is C 1-8 alkyl, C 3-8 cycloalkyl or phenyl. Suitably, R 9A< is C 1-4 alkyl, C 3-8 cycloalkyl or phenyl. Suitably, R 9A< is C 1-4 alkyl such as methyl. Alternatively, R 9A< is C 3-8 cycloalkyl such as C 3-5 cycloalkyl. Alternatively, R 9A< is phenyl.

[0063] In one embodiment, R 9A< is methyl or phenyl, in particular phenyl.

[0064] In one embodiment, R A< contains 6 or more carbon atoms, such as 6, 7, 8, 9 or 10 carbon atoms, such as 8, 9 or 10 carbon atoms. Suitably, R A< contains 6 carbon atoms. Alternatively, R A< contains 7 carbon atoms. Alternatively, R A< contains 8 carbon atoms. Alternatively, R A< contains 9 carbon atoms. Alternatively, R A< contains 10 carbon atoms.

[0065] In one embodiment, R B< is C 1 alkyl substituted by CO 2 H. Suitably, R B< is C 1 alkyl substituted by CO 2 H and the C 1 alkyl carbon of R B< adjacent to the ester oxygen atom is also attached to at least one (such as one) hydrogen atom. In another embodiment, R B< is C 2 alkyl substituted by CO 2 H. Suitably, R B< is C 2 alkyl substituted by CO 2 H and the carbon of R B< adjacent to the ester oxygen atom is also attached to at least one (such as one) hydrogen atom. In any one of these embodiments, suitably R B< is not further substituted by trifluoromethyl or methyl.

[0066] When R B< is substituted by CO 2 H and is optionally further substituted by trifluoromethyl or methyl, the substituent may replace any C-H bond present in R B< . When R B< is substituted by CO 2 H and trifluoromethyl, the substituents may be on the same carbon atom or different carbon atoms. When R B< is substituted by CO 2 H and methyl, the substituents may be on the same carbon atom or different carbon atoms.

[0067] Suitably the CO 2 H group is attached to the terminal carbon i.e. such that the following moiety forms: wherein p is 1 or 2 (e.g. 2).

[0068] When R B< is substituted by CO 2 H and is optionally further substituted by trifluoromethyl or methyl, suitably the carbon atom of R B< adjacent to the ester oxygen atom is also attached to at least one (such as one) hydrogen atom i.e. such that the following moiety forms:

[0069] In one embodiment, R B< is not further substituted.

[0070] In another embodiment, R B< is further substituted by trifluoromethyl. Suitably, the trifluoromethyl group is attached to the carbon atom of R B< adjacent to the ester oxygen atom i.e. such that the following moiety forms:

[0071] In another embodiment, R B< is further substituted by methyl. Suitably, the methyl group is attached to the carbon atom of R B< adjacent to the ester oxygen atom i.e. such that the following moiety forms:

[0072] In one embodiment, R C< and R D< are independently selected from the group consisting of H, C 1-2 alkyl, hydroxy, methoxy and fluoro. Suitably, R C< and R D< are independently selected from the group consisting of H, C 1-2 alkyl, hydroxy and fluoro. In one embodiment, R C< and R D< are independently selected from the group consisting of H, methoxy and fluoro.

[0073] In one embodiment, R C< is H. In one embodiment, R C< is C 1-2 alkyl, in particular methyl. In one embodiment, R C< is hydroxy. In one embodiment, R C< is fluoro. In one embodiment, R C< is methoxy.

[0074] In one embodiment, R D< is H. In one embodiment, R D< is C 1-2 alkyl, in particular methyl. In one embodiment, R D< is hydroxy. In one embodiment, R D< is fluoro. In one embodiment, R D< is methoxy.

[0075] In one embodiment, R C< is H, C 1-2 alkyl (in particular methyl), hydroxy or fluoro; and R D< is H, C 1-2 alkyl (in particular methyl), or fluoro. In one embodiment, R C< is H, C 1-2 alkyl (in particular methyl), hydroxy or fluoro; and R D< is H, C 1-2 alkyl (in particular methyl) or fluoro. In one embodiment, R C< is H, C 1-2 alkyl (in particular methyl), hydroxy or fluoro; and R D< is H or C 1-2 alkyl (in particular methyl). In one embodiment, R C< is H, C 1-2 alkyl (in particular methyl), hydroxy or fluoro; and R D< is H or fluoro. In one embodiment, R C< is H, C 1-2 alkyl (in particular methyl), hydroxy or fluoro; and R D< is H. In one embodiment, R C< is H and R D< is H or C 1-2 alkyl (in particular methyl). In one embodiment, R C< is H and R D< is H or fluoro. In one embodiment, R C< is H or C 1-2 alkyl (in particular methyl); and R D< is H, C 1-2 alkyl (in particular methyl), or fluoro. In one embodiment, R C< is H or C 1-2 alkyl (in particular methyl); and R D< is H or C 1-2 alkyl (in particular methyl). In one embodiment, R C< is H or C 1-2 alkyl (in particular methyl); and R D< is H. In one embodiment, R C< is H and R D< is H. In one embodiment, both of R C< and R D< are not hydroxy. In one embodiment, R C< is methoxy and R D< is H.

[0076] In one embodiment, when R B< contains 2 or more carbon atoms and 4 or more heteroatoms, then R A< must contain 6 or more carbon atoms, and the number of carbon atoms in R A< must exceed the number of heteroatoms in R A< by at least 3 atoms.

[0077] In one embodiment, the molecular weight of the compound of formula (I) is 150 Da - 450 Da.

[0078] In any one of the above embodiments, suitably when R A< is R A< is unsubstituted.

[0079] In one embodiment there is provided a compound of formula (I), selected from the group consisting of: 2-((2-methylene-4-(octyloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-(octyloxy)-4-oxobutanoyl)oxy)propanoic acid; 3-((2-methylene-4-(octyloxy)-4-oxobutanoyl)oxy)propanoic acid; 3-((4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; 3-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; 3-((2-methylene-4-(neopentyloxy)-4-oxobutanoyl)oxy)propanoic acid; (S)-3-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)propanoic acid; 3-((4-(hexyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; 3-((2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoyl)oxy)propanoic acid; 3-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; (R)-3-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)propanoic acid; (S)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-(neopentyloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(hexyloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(spiro[3.3]heptan-2-yloxy)butanoyl)oxy)acetic acid; 2-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; (R)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((4-((4,4-difluorocyclohexyl)methoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(3-ethoxypropoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(bicyclo[2.2.1]heptan-2-yloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-cyclobutoxy-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)-3,3,3-trifluoropropanoic acid; 2-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)-3,3,3-trifluoropropanoic acid; 3-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)-4,4,4-trifluorobutanoic acid; 2-(4-(cycloheptyloxy)-2-methylene-4-oxobutanoyloxy)acetic acid; 2-(2-methylene-4-(octan-3-yloxy)-4-oxobutanoyloxy)acetic acid; 2-(2-methylene-4-(octan-4-yloxy)-4-oxobutanoyloxy)acetic acid; 2-((4-(heptan-4-yloxy)-2-methylene-4-oxobutanoyl)oxy) acetic acid; 2-((4-((adamantan-2-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(1-cyclohexylethoxy)-2-methylene-4-oxobutanoyl)oxy) acetic acid; 2-((4-(1-cycloheptylethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-(2-methylene-4-oxo-4-(spiro[3.4]octan-2-yloxy)butanoyloxy)acetic acid; 2-(2-methylene-4-oxo-4-(spiro[3.5]nonan-2-yloxy)butanoyloxy)acetic acid; 2-(2-methylene-4-oxo-4-(spiro[3.5]nonan-7-yloxy)butanoyloxy)acetic acid; 2-((4-((2,2-dimethylcyclohexyl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(cyclooctyloxy)-3-methyl-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(2,2,4,4-tetramethylcyclobutoxy)butanoyl)oxy) acetic acid; (S)-2-(2-methylene-4-(octan-3-yloxy)-4-oxobutanoyloxy)acetic acid; 2-((4-(cyclooctyloxy)-3-methoxy-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-((-adamantan-1-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (R)-2-((4-(heptan-2-yloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-(nonan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-(nonan-5-yloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((4-(1-(3,5-dichlorophenyl)ethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-((1-(3,5-dichlorophenyl)propan-2-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (R)-2-(2-methylene-4-(octan-3-yloxy)-4-oxobutanoyloxy)acetic acid; 2-((2-methylene-4-oxo-4-(((1R,2S,4R)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)butanoyl)oxy)acetic acid; 2-((4-(1-cyclohexyl-2,2,2-trifluoroethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(bicyclo[3.3.1]nonan-9-yloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (S)-2-((2-methylene-4-oxo-4-((1,1,1-trifluorooctan-2-yl)oxy)butanoyl)oxy)acetic acid; (R)-2-((2-methylene-4-(nonan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid; (R)-2-((4-(1-cyclohexylethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (R)-4,4,4-trifluoro-3-((2-methylene-4-(((R)-octan-2-yl)oxy)-4-oxobutanoyl)oxy)butanoic acid; 2-((4-(cyclooctyloxy)-3-hydroxy-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-(3-methylene-5-(4-methylheptan-4-yloxy)-5-oxopent-1-en-2-yloxy)acetic acid; 2-((4-(1-cyclohexylcyclobutoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-((2-methyloctan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-((2-methylheptan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(1-pentylcyclobutoxy)butanoyl)oxy) acetic acid; 2-((2-methylene-4-((2-methylspiro[3.5]nonan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)butanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-((2,2,6,6-tetramethylcyclohexyl)oxy) butanoyl)oxy)acetic acid; 2-((4-(1-(3,5-dichlorophenyl)ethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid (Isomer 1); 2-((4-(1-(3,5-dichlorophenyl)ethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid (Isomer 2); (R)-2-((2-methylene-4-oxo-4-(1-(4-(trifluoromethyl)phenyl)ethoxy)butanoyl) oxy) acetic acid); (S)-2-((2-methylene-4-oxo-4-(1-(4-(trifluoromethyl)phenyl)ethoxy)butanoyl) oxy) acetic acid); 2-((4-(1-cyclohexylcyclopropoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (S)-2-((4-(1-cyclohexylethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-((2,2,4,4-tetramethylpentan-3-yl)oxy)butanoyl) oxy)acetic acid; (S)-4,4,4-trifluoro-3-((2-methylene-4-(((R)-octan-2-yl)oxy)-4-oxobutanoyl)oxy)butanoic acid; 2-((2-methylene-4-oxo-4-(1-pentylcyclopropoxy)butanoyl)oxy) acetic acid; and 3-((2-methylene-4-oxo-4-(2,2,4,4-tetramethylcyclobutoxy)butanoyl)oxy)propanoic acid; or a pharmaceutically acceptable salt and / or solvate of any one thereof.

[0080] Suitably, the compound is selected from the group consisting of: (R)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid; (R)-3-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)propanoic acid; (S)-2-((2-methylene-4-oxo-4-((1,1,1-trifluorooctan-2-yl)oxy)butanoyl)oxy)acetic acid; and 2-((2-methylene-4-((2-methyloctan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid; or a pharmaceutically acceptable salt and / or solvate of any one thereof.

[0081] Suitably, the compound is selected from the group consisting of: (R)-4,4,4-trifluoro-3-((2-methylene-4-(((R)-octan-2-yl)oxy)-4-oxobutanoyl)oxy)butanoic acid; and (S)-4,4,4-trifluoro-3-((2-methylene-4-(((R)-octan-2-yl)oxy)-4-oxobutanoyl)oxy)butanoic acid; or a pharmaceutically acceptable salt and / or solvate of any one thereof.

[0082] Compounds of formula (I) may be prepared as set out in the Examples, e.g. as set out in General Procedures 1-4.

[0083] For example, compounds of formula (I) may prepared using the following route:

[0084] R A< , R B< , R C< and R D< are as defined elsewhere herein.

[0085] Step (i): itaconate anhydride (V) can be reacted with alcohol (IV), wherein R A'< represents R A< or a protected derivative thereof, in the presence of a catalyst such as p-TsOH.H 2 O in a solvent such as toluene to give monoester (III).

[0086] Step (ii): Monoester (III) and alcohol (II), where R B'< represents R B< or a protected derivative thereof, can be condensed under standard coupling conditions as shown in the General Procedures 2 and 3 to give compounds of formula (I) following any optional deprotection steps. Alternatively, monoester (III) can be reacted with compound (II'), wherein R B'< represents R B< or a protected derivative thereof and X represents a leaving group such as chloro, bromo, iodo, alkanesulfonate or arenesulfonate, in the presence of a base such as potassium carbonate to give compounds of formula (I) after any requisite deprotection steps.

[0087] Compounds of formula (I) may also be prepared by the following route:

[0088] R A< , R B< , R C< and R D< are as defined elsewhere herein.

[0089] Step (i): itaconate anhydride (V) can be reacted with alcohol (VI), wherein PG represents a protecting group orthogonal to any protecting group present in R B'< , in the presence of a catalyst such as p-TsOH.H 2 O in a solvent such as toluene to give monoester (VII).

[0090] Step (ii): Monoester (VII) and alcohol (II), wherein R B'< represents R B< or a protected derivative thereof, can be condensed under standard coupling conditions to give diesters of formula (VIII).

[0091] Step (iii): The orthogonal protecting group PG is removed using conditions known to the person skilled in the art to give itaconate (IX) possessing a free carboxyl group at the 4-position.

[0092] Step (iv): Itaconate (IX) is coupled with alcohol (IV), wherein R A'< represents R A< or a protected derivative thereof, under standard coupling conditions to give monoester (I) following any deprotection steps required.

[0093] Compounds of formula (I) may additionally be made by the following route:

[0094] R A< , R B< , R C< and R D< are as defined elsewhere herein.

[0095] Step (i): Alcohol (IV) is condensed with compound (X), wherein X 1< and X 2< represent leaving groups, such as halo e.g., chloro, bromo or iodo, and R A'< represents R A< or a protected derivative thereof, to give monoester (XI).

[0096] Step (ii): Monoester (XI) is reacted with a trialkylphosphonoacetate of formula (XII), wherein R 11< and R 12< independently represent C 1-4 alkyl optionally substituted with halo and R B'< represents R B< or a protected derivative thereof, to provide a compound of formula (XIII).

[0097] Step (iii): Condensation of a compound of formula (XIII) with formaldehyde or a formaldehyde equivalent thereof e.g., paraformaldehyde, and after any optional deprotection steps, provides the compound of formula (I).

[0098] The skilled person will appreciate that protecting groups may be used throughout the synthetic schemes described herein to give protected derivatives of any of the above compounds or generic formulae. Protective groups and the means for their removal are described in "Protective Groups in Organic Synthesis", by Theodora W. Greene and Peter G. M. Wuts, published by John Wiley & Sons Inc; 4th Rev Ed., 2006, ISBN-10: 0471697540. Examples of nitrogen protecting groups include trityl (Tr), tert-butyloxycarbonyl (BOC), 9-fluorenylmethyloxycarbonyl (Fmoc), acetyl (Ac), benzyl (Bn) and para-methoxy benzyl (PMB). Examples of oxygen protecting groups include acetyl (Ac), methoxymethyl (MOM), para-methoxybenzyl (PMB), benzyl, tert-butyl, methyl, ethyl, tetrahydropyranyl (THP), and silyl ethers and esters (such as trimethylsilyl (TMS), tertbutyldimethylsilyl (TBDMS), tri-iso-propylsilyloxymethyl (TOM), and triisopropylsilyl (TIPS) ethers and esters). Specific examples of carboxylic acid protecting groups include alkyl esters (such as C 1-6 alkyl e.g. C 1-4 alkyl esters), benzyl esters and silyl esters.

[0099] Thus, in one embodiment there is provided a process for preparing a compound of formula (I) or a salt such as a pharmaceutically acceptable salt thereof, which comprises reacting a compound of formula (III): wherein R A'< , R C< and R D< are defined elsewhere herein, or a salt thereof; with a compound of formula (II):         R B'< -OH     (II) wherein R B'< is defined elsewhere herein, or a salt thereof.

[0100] In one embodiment there is provided a process for preparing a compound of formula (I), or a salt such as a pharmaceutically acceptable salt thereof, which comprises reacting a compound of formula (III): wherein R A'< , R C< and R D< are defined elsewhere herein, or a salt thereof; with a compound of formula (II'):         R B'< -X     (II') wherein R B'< and X are defined elsewhere herein, or a salt thereof.

[0101] There is also provided a process for preparing a compound of formula (III) or a salt thereof, which comprises reacting a compound of formula (V): or a salt thereof; with a compound of formula (IV):         R A'< -OH     (IV) wherein R A'< is defined elsewhere herein, or a salt thereof.

[0102] There is also provided a process for preparing a compound of formula (I), or a salt such as a pharmaceutically acceptable salt thereof, which comprises reacting a compound of formula (IX): wherein R B'< , R C< and R D< are defined elsewhere herein, or a salt thereof; with a compound of formula (IV)         R A'< -OH     (IV) wherein R A'< is defined elsewhere herein, or a salt thereof.

[0103] In one embodiment there is provided a process for preparing a compound of formula (VIII) or a salt thereof, which comprises reacting a compound of formula (VII): wherein PG, R C< and R D< are defined elsewhere herein, or a salt thereof; with a compound of formula (II):         R B'< -OH     (II) wherein R B'< is defined elsewhere herein, or a salt thereof.

[0104] In one embodiment there is provided a process for preparing a compound of formula (I), or a salt such as a pharmaceutically acceptable salt thereof, which comprises reacting a compound of formula (XIII): or a salt thereof, with formaldehyde or an equivalent thereof; wherein R A'< , R B'< , R C< , R D< , R 11< and R 12< are defined elsewhere herein.

[0105] In one embodiment there is provided a process for preparing a compound of formula (XIII) or a salt thereof, which comprises reacting a compound of formula (XI): wherein R A'< , R C< , R D< and X 2< are defined elsewhere herein, or a salt thereof; with a compound of formula (XII): wherein R B'< , R 11< and R 12< are defined elsewhere herein, or a salt thereof.

[0106] In one embodiment, there is provided a compound of formula (IX): or a salt thereof, wherein R B'< , R C< and R D< are defined elsewhere herein. Suitably, R B'< represents C 1-2 alkyl substituted by CO 2 H and further substituted by trifluoromethyl or methyl and e.g. represents the group CH(CF 3 )CH 2 CO 2 H or CH(CH 3 )CH 2 CO 2 H, or a corresponding group in which the carboxylic acid is protected.

[0107] In one embodiment, there is provided a compound of formula (XIII): or a salt thereof, wherein R A'< , R B'< , R C< , R D< , R 11< and R 12< are defined elsewhere herein.

[0108] In one embodiment, there is provided a compound of formula (XI): or a salt thereof, wherein R A'< , R C< , R D< and X 2< are defined elsewhere herein.

[0109] Certain intermediates are novel and are claimed as an aspect of the invention: 4-(cyclooctyloxy)-2-methylene-4-oxobutanoic acid; 2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoic acid; 4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoic acid; (R)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid; (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid; 2-methylene-4-(neopentyloxy)-4-oxobutanoic acid; and 3-((2-(tert-butoxy)-2-oxoethoxy)carbonyl)but-3-enoic acid; or salts thereof.

[0110] It will be appreciated that for use in therapy the salts of the compounds of formula (I) should be pharmaceutically acceptable. Suitable pharmaceutically acceptable salts will be apparent to those skilled in the art. Pharmaceutically acceptable salts include acid addition salts, suitably salts of compounds of the invention comprising a basic group such as an amino group, formed with inorganic acids e.g. hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid or phosphoric acid. Also included are salts formed with organic acids e.g. succinic acid, maleic acid, acetic acid, fumaric acid, citric acid, tartaric acid, benzoic acid, p-toluenesulfonic acid, methanesulfonic acid, naphthalenesulfonic acid and 1,5-naphthalenedisulfonic acid. Other salts e.g. oxalates or formates, may be used, for example in the isolation of compounds of formula (I) and are included within the scope of this invention, as are basic addition salts such as sodium, potassium, calcium, aluminium, zinc, magnesium and other metal salts.

[0111] Pharmaceutically acceptable salts may also be formed with organic bases such as basic amines e.g. with ammonia, meglumine, tromethamine, piperazine, arginine, choline, diethylamine, benzathine or lysine. Thus, in one embodiment there is provided a compound of formula (I) in the form of a pharmaceutically acceptable salt. Alternatively, there is provided a compound of formula (I) in the form of a free acid. When the compound contains a basic group as well as the free acid it may be Zwitterionic.

[0112] Suitably, the compound of formula (I) is not a salt e.g. is not a pharmaceutically acceptable salt.

[0113] Suitably, where the compound of formula (I) is in the form of a salt, the pharmaceutically acceptable salt is a basic addition salt such as a carboxylate salt formed with a group 1 metal (e.g. a sodium or potassium salt), a group 2 metal (e.g. a magnesium or calcium salt) or an ammonium salt of a basic amine (e.g. an NH 4 +< salt), such as a sodium salt.

[0114] The compounds of formula (I) may be prepared in crystalline or non-crystalline form and, if crystalline, may optionally be solvated, e.g. as the hydrate. This invention includes within its scope stoichiometric solvates (e.g. hydrates) as well as compounds containing variable amounts of solvent (e.g. water). Suitably, the compound of formula (I) is not a solvate.

[0115] The invention extends to a pharmaceutically acceptable derivative thereof, such as a pharmaceutically acceptable prodrug of compounds of formula (I). Typical prodrugs of compounds of formula (I) which comprise a carboxylic acid include ester (e.g. C 1-6 alkyl e.g. C 1-4 alkyl ester) derivatives thereof. Thus, in one embodiment, the compound of formula (I) is provided as a pharmaceutically acceptable prodrug. In another embodiment, the compound of formula (I) is not provided as a pharmaceutically acceptable prodrug.

[0116] Certain compounds of formula (I) may metabolise under certain conditions such as by hydrolysis of the R B< ester group. Certain metabolites of compounds of formula (I) have activity, as described in Biological Example 6. Without wishing to be bound by theory, formation of an active metabolite (such as in vivo) of a compound of formula (I) may be beneficial by contributing to the biological activity observed of the compound of formula (I). Thus, in one embodiment, there is provided an active metabolite of the compound of formula (I) and its use as a pharmaceutical e.g. for the treatment or prevention of the diseases mentioned herein.

[0117] It is to be understood that the present invention encompasses all isomers of compounds of formula (I) including all geometric, tautomeric and optical forms, and mixtures thereof (e.g. racemic mixtures). Where additional chiral centres are present in compounds of formula (I), the present invention includes within its scope all possible diastereoisomers, including mixtures thereof. The different isomeric forms may be separated or resolved one from the other by conventional methods, or any given isomer may be obtained by conventional synthetic methods or by stereospecific or asymmetric syntheses.

[0118] The present invention also includes all isotopic forms of the compounds provided herein, whether in a form (i) wherein all atoms of a given atomic number have a mass number (or mixture of mass numbers) which predominates in nature (referred to herein as the "natural isotopic form") or (ii) wherein one or more atoms are replaced by atoms having the same atomic number, but a mass number different from the mass number of atoms which predominates in nature (referred to herein as an "unnatural variant isotopic form"). It is understood that an atom may naturally exists as a mixture of mass numbers. The term "unnatural variant isotopic form" also includes embodiments in which the proportion of an atom of given atomic number having a mass number found less commonly in nature (referred to herein as an "uncommon isotope") has been increased relative to that which is naturally occurring e.g. to the level of >20%, >50%, >75%, >90%, >95% or> 99% by number of the atoms of that atomic number (the latter embodiment referred to as an "isotopically enriched variant form"). The term "unnatural variant isotopic form" also includes embodiments in which the proportion of an uncommon isotope has been reduced relative to that which is naturally occurring. Isotopic forms may include radioactive forms (i.e. they incorporate radioisotopes) and non-radioactive forms. Radioactive forms will typically be isotopically enriched variant forms.

[0119] An unnatural variant isotopic form of a compound may thus contain one or more artificial or uncommon isotopes such as deuterium ( 2< H or D), carbon-11 ( 11< C), carbon-13 ( 13< C), carbon-14 ( 14< C), nitrogen-13 ( 13< N), nitrogen-15 ( 15< N), oxygen-15 ( 15< O), oxygen-17 ( 17< O), oxygen-18 ( 18< O), phosphorus-32 ( 32< P), sulphur-35 ( 35< S), chlorine-36 ( 35< Cl), chlorine-37 ( 31< Cl), fluorine-18 ( 13< F) iodine-123 ( 123< I), iodine-125 ( 125< I) in one or more atoms or may contain an increased proportion of said isotopes as compared with the proportion that predominates in nature in one or more atoms.

[0120] Unnatural variant isotopic forms comprising radioisotopes may, for example, be used for drug and / or substrate tissue distribution studies. The radioactive isotopes tritium, i.e. 3< H, and carbon-14, i.e. 14< C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection. Unnatural variant isotopic forms which incorporate deuterium i.e. 2< H or D may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances. Further, unnatural variant isotopic forms may be prepared which incorporate positron emitting isotopes, such as 11< C, 18< F, 15< O and 13< N, and would be useful in positron emission topography (PET) studies for examining substrate receptor occupancy.

[0121] In one embodiment, the compounds of formula (I) are provided in a natural isotopic form. In one embodiment, the compounds of formula (I) are provided in an unnatural variant isotopic form. In a specific embodiment, the unnatural variant isotopic form is a form in which deuterium (i.e. 2< H or D) is incorporated where hydrogen is specified in the chemical structure in one or more atoms of a compound of formula (I). In one embodiment, the atoms of the compounds of formula (I) are in an isotopic form which is not radioactive. In one embodiment, one or more atoms of the compounds of formula (I) are in an isotopic form which is radioactive. Suitably radioactive isotopes are stable isotopes. Suitably the unnatural variant isotopic form is a pharmaceutically acceptable form.

[0122] In one embodiment, a compound of formula (I) is provided whereby a single atom of the compound exists in an unnatural variant isotopic form. In another embodiment, a compound of formula (I) is provided whereby two or more atoms exist in an unnatural variant isotopic form.

[0123] Unnatural isotopic variant forms can generally be prepared by conventional techniques known to those skilled in the art or by processes described herein e.g. processes analogous to those described in the accompanying Examples for preparing natural isotopic forms. Thus, unnatural isotopic variant forms could be prepared by using appropriate isotopically variant (or labelled) reagents in place of the normal reagents employed in the Examples. Since the compounds of formula (I) are intended for use in pharmaceutical compositions it will readily be understood that they are each preferably provided in substantially pure form, for example at least 60% pure, more suitably at least 75% pure and preferably at least 85%, especially at least 98% pure (% are on a weight for weight basis). Impure preparations of the compounds may be used for preparing the more pure forms used in the pharmaceutical compositions.Therapeutic indications

[0124] Compounds of formula (I) are of use in therapy, particularly for treating or preventing an inflammatory disease or a disease associated with an undesirable immune response. As shown in Biological Example 1 below, example compounds of formula (I) reduced cytokine release more effectively than dimethyl itaconate, as demonstrated by lower IC 50 values. Cytokines are important mediators of inflammation and immune-mediated disease as evidenced by the therapeutic benefit delivered by antibodies targeting them.

[0125] Thus, in a first aspect, the present invention provides a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof as defined herein, for use as a medicament. Also provided is a pharmaceutical composition comprising a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof as defined herein. Such a pharmaceutical composition contains the compound of formula (I) and a pharmaceutically acceptable carrier or excipient.

[0126] In a further aspect, the present invention provides a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof as defined herein, for use in treating or preventing an inflammatory disease or a disease associated with an undesirable immune response.

[0127] For all aspects of the invention, suitably the compound is administered to a subject in need thereof, wherein the subject is suitably a human subject.

[0128] In one embodiment is provided a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof as defined herein, for use in treating an inflammatory disease or disease associated with an undesirable immune response.

[0129] In one embodiment is provided a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof as defined herein, for use in preventing an inflammatory disease or a disease associated with an undesirable immune response.

[0130] In one embodiment is provided a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof as defined herein, for use in treating or preventing an inflammatory disease.

[0131] In one embodiment is provided a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof as defined herein, for use in treating or preventing a disease associated with an undesirable immune response.

[0132] An undesirable immune response will typically be an immune response which gives rise to a pathology i.e. is a pathological immune response or reaction.

[0133] In one embodiment, the inflammatory disease or disease associated with an undesirable immune response is an auto-immune disease.

[0134] In one embodiment, the inflammatory disease or disease associated with an undesirable immune response is, or is associated with, a disease selected from the group consisting of: psoriasis (including chronic plaque, erythrodermic, pustular, guttate, inverse and nail variants), asthma, chronic obstructive pulmonary disease (COPD, including chronic bronchitis and emphysema), heart failure (including left ventricular failure), myocardial infarction, angina pectoris, other atherosclerosis and / or atherothrombosis-related disorders (including peripheral vascular disease and ischaemic stroke), a mitochondrial and neurodegenerative disease (such as Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, retinitis pigmentosa or mitochondrial encephalomyopathy), autoimmune paraneoplastic retinopathy, transplantation rejection (including antibody-mediated and T cell-mediated forms), multiple sclerosis, transverse myelitis, ischaemia-reperfusion injury (e.g. during elective surgery such as cardiopulmonary bypass for coronary artery bypass grafting or other cardiac surgery, following percutaneous coronary intervention, following treatment of acute ST-elevation myocardial infarction or ischaemic stroke, organ transplantation, or acute compartment syndrome), AGE-induced genome damage, an inflammatory bowel disease (e.g. Crohn's disease or ulcerative colitis), primary sclerosing cholangitis (PSC), PSC-autoimmune hepatitis overlap syndrome, non-alcoholic fatty liver disease (non-alcoholic steatohepatitis), rheumatica, granuloma annulare, cutaneous lupus erythematosus (CLE), systemic lupus erythematosus (SLE), lupus nephritis, drug-induced lupus, autoimmune myocarditis or myopericarditis, Dressler's syndrome, giant cell myocarditis, post-pericardiotomy syndrome, drug-induced hypersensitivity syndromes (including hypersensitivity myocarditis), eczema, sarcoidosis, erythema nodosum, acute disseminated encephalomyelitis (ADEM), neuromyelitis optica spectrum disorders, MOG (myelin oligodendrocyte glycoprotein) antibody-associated disorders (including MOG-EM), optic neuritis, CLIPPERS (chronic lymphocytic inflammation with pontine perivascular enhancement responsive to steroids), diffuse myelinoclastic sclerosis, Addison's disease, alopecia areata, ankylosing spondylitis, other spondyloarthritides (including peripheral spondyloarthritis, that is associated with psoriasis, inflammatory bowel disease, reactive arthritis or juvenile onset forms), antiphospholipid antibody syndrome, autoimmune hemolytic anaemia, autoimmune hepatitis, autoimmune inner ear disease, pemphigoid (including bullous pemphigoid, mucous membrane pemphigoid, cicatricial pemphigoid, herpes gestationis or pemphigoid gestationis, ocular cicatricial pemphigoid), linear IgA disease, Behçet's disease, celiac disease, Chagas disease, dermatomyositis, diabetes mellitus type I, endometriosis, Goodpasture's syndrome, Graves' disease, Guillain-Barre syndrome and its subtypes (including acute inflammatory demyelinating polyneuropathy, AIDP, acute motor axonal neuropathy (AMAN), acute motor and sensory axonal neuropathy (AMSAN), pharyngeal-cervical-brachial variant, Miller-Fisher variant and Bickerstaff's brainstem encephalitis), progressive inflammatory neuropathy, Hashimoto's disease, hidradenitis suppurativa, inclusion body myositis, necrotising myopathy, Kawasaki disease, IgA nephropathy, Henoch-Schonlein purpura, idiopathic thrombocytopenic purpura, thrombotic thrombocytopenic purpura (TTP), Evans' syndrome, interstitial cystitis, mixed connective tissue disease, undifferentiated connective tissue disease, morphea, myasthenia gravis (including MuSK antibody positive and seronegative variants), narcolepsy, neuromyotonia, pemphigus vulgaris, pernicious anaemia, psoriatic arthritis, polymyositis, primary biliary cholangitis (also known as primary biliary cirrhosis), rheumatoid arthritis, palindromic rheumatism, schizophrenia, autoimmune (meningo-)encephalitis syndromes, scleroderma, Sjogren's syndrome, stiff person syndrome, polymylagia rheumatica, giant cell arteritis (temporal arteritis), Takayasu arteritis, polyarteritis nodosa, Kawasaki disease, granulomatosis with polyangitis (GPA; formerly known as Wegener's granulomatosis), eosinophilic granulomatosis with polyangiitis (EGPA; formerly known as Churg-Strauss syndrome), microscopic polyarteritis / polyangiitis, hypocomplementaemic urticarial vasculitis, hypersensitivity vasculitis, cryoglobulinemia, thromboangiitis obliterans (Buerger's disease), vasculitis, leukocytoclastic vasculitis, vitiligo, acute disseminated encephalomyelitis, adrenoleukodystrophy, Alexander's disease, Alper's disease, balo concentric sclerosis or Marburg disease, cryptogenic organising pneumonia (formerly known as bronchiolitis obliterans organizing pneumonia), Canavan disease, central nervous system vasculitic syndrome, Charcot-Marie-Tooth disease, childhood ataxia with central nervous system hypomyelination, chronic inflammatory demyelinating polyneuropathy (CIDP), diabetic retinopathy, globoid cell leukodystrophy (Krabbe disease), graft-versus-host disease (GVHD) (including acute and chronic forms, as well as intestinal GVHD), hepatitis C (HCV) infection or complication, herpes simplex viral infection or complication, human immunodeficiency virus (HIV) infection or complication, lichen planus, monomelic amyotrophy, cystic fibrosis, pulmonary arterial hypertension (PAH, including idiopathic PAH), lung sarcoidosis, idiopathic pulmonary fibrosis, paediatric asthma, atopic dermatitis, allergic dermatitis, contact dermatitis, allergic rhinitis, rhinitis, sinusitis, conjunctivitis, allergic conjunctivitis, keratoconjunctivitis sicca, dry eye, xerophthalmia, glaucoma, macular oedema, diabetic macular oedema, central retinal vein occlusion (CRVO), macular degeneration (including dry and / or wet age related macular degeneration, AMD), post-operative cataract inflammation, uveitis (including posterior, anterior, intermediate and pan uveitis), iridocyclitis, scleritis, corneal graft and limbal cell transplant rejection, gluten sensitive enteropathy (coeliac disease), dermatitis herpetiformis, eosinophilic esophagitis, achalasia, autoimmune dysautonomia, autoimmune encephalomyelitis, autoimmune oophoritis, autoimmune orchitis, autoimmune pancreatitis, aortitis and periaortitis, autoimmune retinopathy, autoimmune urticaria, Behcet's disease, (idiopathic) Castleman's disease, Cogan's syndrome, IgG4-related disease, retroperitoneal fibrosis, juvenile idiopathic arthritis including systemic juvenile idiopathic arthritis (Still's disease), adult-onset Still's disease, ligneous conjunctivitis, Mooren's ulcer, pityriasis lichenoides et varioliformis acuta (PLEVA, also known as Mucha-Habermann disease), multifocal motor neuropathy (MMN), paediatric acute-onset neuropsychiatric syndrome (PANS) (including paediatric autoimmune neuropsychiatric disorders associated with streptococcal infections (PANDAS)), paraneoplastic syndromes (including paraneoplastic cerebellar degeneration, Lambert-Eaton myaesthenic syndrome, limbic encephalitis, brainstem encephalitis, opsoclonus myoclonus ataxia syndrome, anti-NMDA receptor encephalitis, thymoma-associated multiorgan autoimmunity), perivenous encephalomyelitis, reflex sympathetic dystrophy, relapsing polychondritis, sperm & testicular autoimmunity, Susac's syndrome, Tolosa-Hunt syndrome, Vogt-Koyanagi-Harada Disease, anti-synthetase syndrome, autoimmune enteropathy, immune dysregulation polyendocrinopathy enteropathy X-linked (IPEX), microscopic colitis, autoimmune lymphoproliferative syndrome (ALPS), autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy syndrome (APEX), gout, pseudogout, amyloid (including AA or secondary amyloidosis), eosinophilic fasciitis (Shulman syndrome) progesterone hypersensitivity (including progesterone dermatitis), familial Mediterranean fever (FMF), tumour necrosis factor (TNF) receptor-associated periodic fever syndrome (TRAPS), hyperimmunoglobulinaemia D with periodic fever syndrome (HIDS), PAPA (pyogenic arthritis, pyoderma gangrenosum, severe cystic acne) syndrome, deficiency of interleukin-1 receptor antagonist (DIRA), deficiency of the interleukin-36-receptor antagonist (DITRA), cryopyrin-associated periodic syndromes (CAPS) (including familial cold autoinflammatory syndrome [FCAS], Muckle-Wells syndrome, neonatal onset multisystem inflammatory disease [NOMID]), NLRP12-associated autoinflammatory disorders (NLRP12AD), periodic fever aphthous stomatitis (PFAPA), chronic atypical neutrophilic dermatosis with lipodystrophy and elevated temperature (CANDLE), Majeed syndrome, Blau syndrome (also known as juvenile systemic granulomatosis), macrophage activation syndrome, chronic recurrent multifocal osteomyelitis (CRMO), familial cold autoinflammatory syndrome, mutant adenosine deaminase 2 and monogenic interferonopathies (including Aicardi-Goutières syndrome, retinal vasculopathy with cerebral leukodystrophy, spondyloenchondrodysplasia, STING [stimulator of interferon genes]-associated vasculopathy with onset in infancy, proteasome associated autoinflammatory syndromes, familial chilblain lupus, dyschromatosis symmetrica hereditaria), Schnitzler syndrome; familial cylindromatosis, congenital B cell lymphocytosis, OTULIN-related autoinflammatory syndrome, type 2 diabetes mellitus, insulin resistance and the metabolic syndrome (including obesity-associated inflammation), atherosclerotic disorders (e.g. myocardial infarction, angina, ischaemic heart failure, ischaemic nephropathy, ischaemic stroke, peripheral vascular disease, aortic aneurysm), renal inflammatory disorders (e.g. diabetic nephropathy, membranous nephropathy, minimal change disease, crescentic glomerulonephritis, acute kidney injury, renal transplantation).

[0135] In one embodiment, the inflammatory disease or disease associated with an undesirable immune response is, or is associated with, a disease selected from the following autoinflammatory diseases: familial Mediterranean fever (FMF), tumour necrosis factor (TNF) receptor-associated periodic fever syndrome (TRAPS), hyperimmunoglobulinaemia D with periodic fever syndrome (HIDS), PAPA (pyogenic arthritis, pyoderma gangrenosum, and severe cystic acne) syndrome, deficiency of interleukin-1 receptor antagonist (DIRA), deficiency of the interleukin-36-receptor antagonist (DITRA), cryopyrin-associated periodic syndromes (CAPS) (including familial cold autoinflammatory syndrome [FCAS], Muckle-Wells syndrome, and neonatal onset multisystem inflammatory disease [NOMID]), NLRP12-associated autoinflammatory disorders (NLRP12AD), periodic fever aphthous stomatitis (PFAPA), chronic atypical neutrophilic dermatosis with lipodystrophy and elevated temperature (CANDLE), Majeed syndrome, Blau syndrome (also known as juvenile systemic granulomatosis), macrophage activation syndrome, chronic recurrent multifocal osteomyelitis (CRMO), familial cold autoinflammatory syndrome, mutant adenosine deaminase 2 and monogenic interferonopathies (including Aicardi-Goutières syndrome, retinal vasculopathy with cerebral leukodystrophy, spondyloenchondrodysplasia, STING [stimulator of interferon genes]-associated vasculopathy with onset in infancy, proteasome associated autoinflammatory syndromes, familial chilblain lupus, dyschromatosis symmetrica hereditaria) and Schnitzler syndrome.

[0136] In one embodiment, the inflammatory disease or disease associated with an undesirable immune response is, or is associated with, a disease selected from the following diseases mediated by excess NF-κB or gain of function in the NF-κB signalling pathway or in which there is a major contribution to the abnormal pathogenesis therefrom (including non-canonical NF-κB signalling): familial cylindromatosis, congenital B cell lymphocytosis, OTULIN-related autoinflammatory syndrome, type 2 diabetes mellitus, insulin resistance and the metabolic syndrome (including obesity-associated inflammation), atherosclerotic disorders (e.g. myocardial infarction, angina, ischaemic heart failure, ischaemic nephropathy, ischaemic stroke, peripheral vascular disease, aortic aneurysm), renal inflammatory disorders (e.g. diabetic nephropathy, membranous nephropathy, minimal change disease, crescentic glomerulonephritis, acute kidney injury, renal transplantation), asthma, COPD, type 1 diabetes mellitus, rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease (including ulcerative colitis and Crohn's disease), and SLE.

[0137] In one embodiment, the disease is selected from the group consisting of rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, systemic lupus erythematosus, multiple sclerosis, psoriasis, Crohn's disease, ulcerative colitis, uveitis, cryopyrin-associated periodic syndromes, Muckle-Wells syndrome, juvenile idiopathic arthritis and chronic obstructive pulmonary disease. In one embodiment, the disease is multiple sclerosis.

[0138] In one embodiment, the disease is psoriasis.

[0139] In one embodiment, the compound of formula (I) exhibits a lower IC 50 compared with dimethyl itaconate when tested in a cytokine assay e.g. as described in Biological Example 1. In one embodiment, the compound of formula (I) exhibits a lower IC 50 compared with dimethyl fumarate when tested in a cytokine assay e.g. as described in Biological Example 1.

[0140] In one embodiment, the compound of formula (I) exhibits a lower EC 50 compared with dimethyl itaconate when tested in an NRF2 assay e.g. as described in Biological Example 3. In one embodiment, the compound of formula (I) exhibits a higher E max compared with dimethyl itaconate when tested in an NRF2 assay e.g. as described in Biological Example 3. In one embodiment, the compound of formula (I) exhibits a lower EC 50 and / or higher E max compared with dimethyl itaconate when tested in an NRF2 assay e.g. as described in Biological Example 3. In one embodiment, the compound of formula (I) exhibits a lower EC 50 and higher E max compared with dimethyl itaconate when tested in an NRF2 assay e.g. as described in Biological Example 3.

[0141] In one embodiment, the compound of formula (I) exhibits improved oral systemic bioavailability compared with dimethyl itaconate e.g. as described in Biological Example 4. In one embodiment, the compound of formula (I) exhibits reduced plasma clearance following intravenous dosing compared with dimethyl itaconate e.g. as described in Biological Example 4.

[0142] In one embodiment, the compound of formula (I) exhibits lower intrinsic clearance (Cl int ) compared with 4-octyl itaconate when tested in a hepatocyte stability assay, e.g., as described in Biological Example 5. In one embodiment, the compound of formula (I) exhibits a longer half-life (T1 / 2) compared with 4-octyl itaconate when tested in a hepatocyte stability assay, e.g. as described in Biological Example 5.Administration

[0143] The compound of formula (I) is usually administered as a pharmaceutical composition. Thus, in one embodiment, is provided a pharmaceutical composition comprising a compound of formula (I) and one or more pharmaceutically acceptable diluents or carriers.

[0144] The compound of formula (I) may be administered by any convenient method, e.g. by oral, parenteral, buccal, sublingual, nasal, rectal, intrathecal or transdermal administration, and the pharmaceutical compositions adapted accordingly.

[0145] The compound of formula (I) may be administered topically to the target organ e.g. topically to the eye, lung, nose or skin. Hence the invention provides a pharmaceutical composition comprising a compound of formula (I) optionally in combination with one or more topically acceptable diluents or carriers.

[0146] A compound of formula (I) which is active when given orally can be formulated as a liquid or solid, e.g. as a syrup, suspension, emulsion, tablet, capsule or lozenge.

[0147] A liquid formulation will generally consist of a suspension or solution of the compound of formula (I) in a suitable liquid carrier(s). Suitably the carrier is non-aqueous e.g. polyethylene glycol or an oil. The formulation may also contain a suspending agent, preservative, flavouring and / or colouring agent.

[0148] A composition in the form of a tablet can be prepared using any suitable pharmaceutical carrier(s) routinely used for preparing solid formulations, such as magnesium stearate, starch, lactose, sucrose and cellulose.

[0149] A composition in the form of a capsule can be prepared using routine encapsulation procedures, e.g. pellets containing the active ingredient can be prepared using standard carriers and then filled into a hard gelatine capsule; alternatively, a dispersion or suspension can be prepared using any suitable pharmaceutical carrier(s), e.g. aqueous gums, celluloses, silicates or oils and the dispersion or suspension then filled into a soft gelatine capsule.

[0150] Typical parenteral compositions consist of a solution or suspension of the compound of formula (I) in a sterile aqueous carrier or parenterally acceptable oil, e.g. polyethylene glycol, polyvinyl pyrrolidone, lecithin, arachis oil or sesame oil. Alternatively, the solution can be lyophilised and then reconstituted with a suitable solvent just prior to administration.

[0151] Compositions for nasal administration may conveniently be formulated as aerosols, drops, gels and powders. Aerosol formulations typically comprise a solution or fine suspension of the compound of formula (I) in a pharmaceutically acceptable aqueous or non-aqueous solvent and are usually presented in single or multidose quantities in sterile form in a sealed container which can take the form of a cartridge or refill for use with an atomising device. Alternatively, the sealed container may be a disposable dispensing device such as a single dose nasal inhaler or an aerosol dispenser fitted with a metering valve. Where the dosage form comprises an aerosol dispenser, it will contain a propellant which can be a compressed gas e.g. air, or an organic propellant such as a chlorofluorocarbon (CFC) or a hydrofluorocarbon (HFC). Aerosol dosage forms can also take the form of pump-atomisers.

[0152] Topical administration to the lung may be achieved by use of an aerosol formulation. Aerosol formulations typically comprise the active ingredient suspended or dissolved in a suitable aerosol propellant, such as a chlorofluorocarbon (CFC) or a hydrofluorocarbon (HFC).

[0153] Topical administration to the lung may also be achieved by use of a non-pressurised formulation such as an aqueous solution or suspension. These may be administered by means of a nebuliser e.g. one that can be hand-held and portable or for home or hospital use (i.e. non-portable). The formulation may comprise excipients such as water, buffers, tonicity adjusting agents, pH adjusting agents, surfactants and co-solvents.

[0154] Topical administration to the lung may also be achieved by use of a dry-powder formulation. The formulation will typically contain a topically acceptable diluent such as lactose, glucose or mannitol (preferably lactose).

[0155] The compound of the invention may also be administered rectally, for example in the form of suppositories or enemas, which include aqueous or oily solutions as well as suspensions and emulsions and foams. Such compositions are prepared following standard procedures, well known by those skilled in the art. For example, suppositories can be prepared by mixing the active ingredient with a conventional suppository base such as cocoa butter or other glycerides. In this case, the drug is mixed with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug. Such materials are cocoa butter and polyethylene glycols.

[0156] Generally, for compositions intended to be administered topically to the eye in the form of eye drops or eye ointments, the total amount of the compound of the present invention will be about 0.0001 to less than 4.0% (w / w).

[0157] Preferably, for topical ocular administration, the compositions administered according to the present invention will be formulated as solutions, suspensions, emulsions and other dosage forms.

[0158] The compositions administered according to the present invention may also include various other ingredients, including, but not limited to, tonicity agents, buffers, surfactants, stabilizing polymer, preservatives, co-solvents and viscosity building agents. Suitable pharmaceutical compositions of the present invention include a compound of the invention formulated with a tonicity agent and a buffer. The pharmaceutical compositions of the present invention may further optionally include a surfactant and / or a palliative agent and / or a stabilizing polymer.

[0159] Various tonicity agents may be employed to adjust the tonicity of the composition, preferably to that of natural tears for ophthalmic compositions. For example, sodium chloride, potassium chloride, magnesium chloride, calcium chloride, simple sugars such as dextrose, fructose, galactose, and / or simply polyols such as the sugar alcohols mannitol, sorbitol, xylitol, lactitol, isomaltitol, maltitol, and hydrogenated starch hydrolysates may be added to the composition to approximate physiological tonicity. Such an amount of tonicity agent will vary, depending on the particular agent to be added. In general, however, the compositions will have a tonicity agent in an amount sufficient to cause the final composition to have an ophthalmically acceptable osmolality (generally about 150-450 mOsm, preferably 250-350 mOsm and most preferably at approximately 290 mOsm). In general, the tonicity agents of the invention will be present in the range of 2 to 4% w / w. Preferred tonicity agents of the invention include the simple sugars or the sugar alcohols, such as D-mannitol.

[0160] An appropriate buffer system (e.g. sodium phosphate, sodium acetate, sodium citrate, sodium borate or boric acid) may be added to the compositions to prevent pH drift under storage conditions. The particular concentration will vary, depending on the agent employed. Preferably however, the buffer will be chosen to maintain a target pH within the range of pH 5 to 8, and more preferably to a target pH of pH 5 to 7.

[0161] Surfactants may optionally be employed to deliver higher concentrations of compound of the present invention. The surfactants function to solubilise the compound and stabilise colloid dispersion, such as micellar solution, microemulsion, emulsion and suspension. Examples of surfactants which may optionally be used include polysorbate, poloxamer, polyosyl 40 stearate, polyoxyl castor oil, tyloxapol, Triton, and sorbitan monolaurate. Preferred surfactants to be employed in the invention have a hydrophile / lipophile / balance "HLB" in the range of 12.4 to 13.2 and are acceptable for ophthalmic use, such as TritonX114 and tyloxapol.

[0162] Additional agents that may be added to the ophthalmic compositions of compounds of the present invention are demulcents which function as a stabilising polymer. The stabilizing polymer should be an ionic / charged example with precedence for topical ocular use, more specifically, a polymer that carries negative charge on its surface that can exhibit a zeta-potential of (-)10-50 mV for physical stability and capable of making a dispersion in water (i.e. water soluble). A preferred stabilising polymer of the invention would be polyelectrolyte, or polyelectrolytes if more than one, from the family of cross-linked polyacrylates, such as carbomers and Pemulen(R), specifically Carbomer 974p (polyacrylic acid), at 0.1-0.5% w / w.

[0163] Other compounds may also be added to the ophthalmic compositions of the compound of the present invention to increase the viscosity of the carrier. Examples of viscosity enhancing agents include, but are not limited to: polysaccharides, such as hyaluronic acid and its salts, chondroitin sulfate and its salts, dextrans, various polymers of the cellulose family; vinyl polymers; and acrylic acid polymers.

[0164] Topical ophthalmic products are typically packaged in multidose form. Preservatives are thus required to prevent microbial contamination during use. Suitable preservatives include: benzalkonium chloride, chlorobutanol, benzododecinium bromide, methyl paraben, propyl paraben, phenylethyl alcohol, edentate disodium, sorbic acid, polyquaternium-1, or other agents known to those skilled in the art. Such preservatives are typically employed at a level of from 0.001 to 1.0% w / v. Unit dose compositions of the present invention will be sterile, but typically unpreserved. Such compositions, therefore, generally will not contain preservatives.

[0165] Compositions suitable for buccal or sublingual administration include tablets, lozenges and pastilles where the compound of formula (I) is formulated with a carrier such as sugar and acacia, tragacanth, or gelatine and glycerine.

[0166] Compositions suitable for transdermal administration include ointments, gels and patches.

[0167] The composition may contain from 0.1% to 100% by weight, for example from 10 to 60% by weight, of the compound of formula (I), depending on the method of administration. The composition may contain from 0% to 99% by weight, for example 40% to 90% by weight, of the carrier, depending on the method of administration. The composition may contain from 0.05mg to 1000mg, for example from 1.0 mg to 500 mg, such as from 1.0 mg to 50 mg, e.g. about 10 mg of the compound of formula (I), depending on the method of administration. The composition may contain from 50 mg to 1000 mg, for example from 100mg to 400mg of the carrier, depending on the method of administration. The dose of the compound used in the treatment of the aforementioned disorders will vary in the usual way with the seriousness of the disorders, the weight of the sufferer, and other similar factors. However, as a general guide suitable unit doses may be 0.05 to 1000 mg, more suitably 1.0 to 500mg, such as from 1.0 mg to 50 mg, e.g. about 10 mg and such unit doses may be administered more than once a day, for example two or three times a day. Such therapy may extend for a number of weeks or months.

[0168] In one embodiment of the invention, the compound of formula (I) is used in combination with a further therapeutic agent or agents. When the compound of formula (I) is used in combination with other therapeutic agents, the compounds may be administered either sequentially or simultaneously by any convenient route. Alternatively, the compounds may be administered separately.

[0169] Therapeutic agents which may be used in combination with the present invention include: corticosteroids (glucocorticoids), retinoids (e.g. acitretin, isotretinoin, tazarotene), anthralin, vitamin D analogues (e.g. cacitriol, calcipotriol), calcineurin inhibitors (e.g. tacrolimus, pimecrolimus), phototherapy or photochemotherapy (e.g. psoralen ultraviolet irradiation, PUVA) or other form of ultraviolet light irradiation therapy, ciclosporine, thiopurines (e.g. azathioprine, 6-mercaptopurine), methotrexate, anti-TNFα agents (e.g. infliximab, etanercept, adalimumab, certolizumab, golimumab and biosimilars), phosphodiesterase-4 (PDE4) inhibition (e.g. apremilast, crisaborole), anti-IL-17 agents (e.g. brodalumab, ixekizumab, secukinumab), anti-IL12 / IL-23 agents (e.g. ustekinumab, briakinumab), anti-IL-23 agents (e.g. guselkumab, tildrakizumab), JAK (Janus Kinase) inhibitors (e.g. tofacitinib, ruxolitinib, baricitinib, filgotinib, upadacitinib), plasma exchange, intravenous immune globulin (IVIG), cyclophosphamide, anti-CD20 B cell depleting agents (e.g. rituximab, ocrelizumab, ofatumumab, obinutuzumab), anthracycline analogues (e.g. mitoxantrone), cladribine, sphingosine 1-phosphate receptor modulators or sphingosine analogues (e.g. fingolimod, siponimod, ozanimod, etrasimod), interferon beta preparations (including interferon beta 1b / 1a), glatiramer, anti-CD3 therapy (e.g. OKT3), anti-CD52 targeting agents (e.g. alemtuzumab), leflunomide, teriflunomide, gold compounds, laquinimod, potassium channel blockers (e.g. dalfampridine / 4-aminopyridine), mycophenolic acid, mycophenolate mofetil, purine analogues (e.g. pentostatin), mTOR (mechanistic target of rapamycin) pathway inhibitors (e.g. sirolimus, everolimus), anti-thymocyte globulin (ATG), IL-2 receptor (CD25) inhibitors (e.g. basiliximab, daclizumab), anti-IL-6 receptor or anti-IL-6 agents (e.g. tocilizumab, siltuximab), Bruton's tyrosine kinase (BTK) inhibitors (e.g. ibrutinib), tyrosine kinase inhibitors (e.g. imatinib), ursodeoxycholic acid, hydroxychloroquine, chloroquine, B cell activating factor (BAFF, also known as BLyS, B lymphocyte stimulator) inhibitors (e.g. belimumab, blisibimod), other B cell targeted therapy including fusion proteins targeting both APRIL (A PRoliferation-Inducing Ligand) and BLyS (e.g. atacicept), PI3K inhibitors including pan-inhibitors or those targeting the p110δ and / or p110y containing isoforms (e.g. idelalisib, copanlisib, duvelisib), interferon α receptor inhibitors (e.g. anifrolumab, sifalimumab), T cell co-stimulation blockers (e.g. abatacept, belatacept), thalidomide and its derivatives (e.g. lenalidomide), dapsone, clofazimine, leukotriene antagonists (e.g. montelukast), theophylline, anti-IgE therapy (e.g. omalizumab), anti-IL-5 agents (e.g. mepolizumab, reslizumab), long-acting muscarinic agents (e.g. tiotropium, aclidinium, umeclidinium), PDE4 inhibitors (e.g. roflumilast), riluzole, free radical scavengers (e.g. edaravone), proteasome inhibitors (e.g. bortezomib), complement cascade inhibitors including those directed against C5 (e.g. eculizumab), immunoadsor, antithymocyte globulin, 5-aminosalicylates and their derivatives (e.g. sulfasalazine, balsalazide, mesalamine), anti-integrin agents including those targeting α4β1 and / or α4β7 integrins (e.g. natalizumab, vedolizumab), anti-CD11-α agents (e.g. efalizumab), non-steroidal anti-inflammatory drugs (NSAIDs) including the salicylates (e.g. aspirin), propionic acids (e.g. ibuprofen, naproxen), acetic acids (e.g. indomethacin, diclofenac, etodolac), oxicams (e.g. meloxicam) and fenamates (e.g. mefenamic acid), selective or relatively selective COX-2 inhibitors (e.g. celecoxib, etroxicoxib, valdecoxib and etodolac, meloxicam, nabumetone), colchicine, IL-4 receptor inhibitors (e.g. dupilumab), topical / contact immunotherapy (e.g. diphenylcyclopropenone, squaric acid dibutyl ester), anti-IL-1 receptor therapy (e.g. anakinra), IL-1β inhibitor (e.g. canakinumab), IL-1 neutralising therapy (e.g. rilonacept), chlorambucil, specific antibiotics with immunomodulatory properties and / or ability to modulate NRF2 (e.g. tetracyclines including minocycline, clindamycin, macrolide antibiotics), anti-androgenic therapy (e.g. cyproterone, spironolactone, finasteride), pentoxifylline, ursodeoxycholic acid, obeticholic acid, fibrate, cystic fibrosis transmembrane conductance regulator (CFTR) modulators, VEGF (vascular endothelial growth factor) inhibitors (e.g. bevacizumab, ranibizumab, pegaptanib, aflibercept), pirfenidone, and mizoribine.

[0170] Compounds of formula (I) may display one or more of the following desirable properties: low IC 50 values for inhibiting release of cytokines e.g. IL-1β and / or IL-6, from cells; low EC 50 and / or high E max values for activating the NRF2 pathway; enhanced efficacy through improved hydrolytic stability of carboxylic acid esters and / or augmented maximum response; reduced dose and dosing frequency through improved pharmacokinetics; improved oral systemic bioavailability; reduced plasma clearance following intravenous dosing; improved metabolic stability e.g. as demonstrated by improved stability in plasma and / or hepatocytes; augmented cell permeability; enhanced aqueous solubility; good tolerability, for example, by limiting the flushing and / or gastrointestinal side effects provoked by oral DMF (Hunt T. et al., 2015; WO2014 / 152494A1), possibly by reducing or eliminating HCA2 activity; low toxicity at the relevant therapeutic dose; distinct anti-inflammatory profiles resulting from varied electrophilicities, leading to differential targeting of the cysteine proteome (van der Reest J. et al., 2018) and, therefore, modified effects on gene activation; glutathione-sparing actions; avoiding the oncometabolite fumaric acid (Kulkarni R. A. et al., 2019). Abbreviations

[0171] Ac 2 Oacetic anhydride ADEMacute disseminated encephalomyelitis AIDPacute inflammatory demyelinating polyneuropathy ALPSautoimmune lymphoproliferative syndrome AMANacute motor axonal neuropathy AMDage related macular degeneration AMSANacute motor and sensory axonal neuropathy APEXautoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy syndrome APRILA PRoliferation-Inducing Ligand aq.aqueous ATF3activating transcription factor 3 ATGanti-thymocyte BAFFB cell activating factor BBFObroadband fluorine observe BEHethylene bridged hybrid Boctertiary-butoxycarbonyl BSAbovine serum albumin BTKBruton's tyrosine kinase CACcitric acid cycle CANDLEchronic atypical neutrophilic dermatosis with lipodystrophy and elevated temperature CAPScryopyrin-associated periodic syndromes CFCchlorofluorocarbon CFTRcystic fibrosis transmembrane conductance regulator CIDPchronic inflammatory demyelinating polyneuropathy CLEcutaneous lupus erythematosus CLIPPERSchronic lymphocytic inflammation with pontine perivascular enhancement responsive to steroids CLLchronic lymphocytic leukaemia COPDchronic obstructive pulmonary disease CRMOchronic recurrent multifocal osteomyelitis CRVOcentral retinal vein occlusion CSHcharged surface hybrid DABCO1,4-diazabicyclo[2.2.2]octane DADdiode array detector DBU1,8-diazabicyclo(5.4.0)undec-7-ene DCCN,N'-dicyclohexylcarbodiimide DCMdichloromethane DIPEAN,N-diisopropylethylamine DIRAdeficiency of interleukin-1 receptor antagonist DITRAdeficiency of the interleukin-36-receptor antagonist DLBCLdiffuse large B cell lymphoma DMAP4-dimethylaminopyridine DMFdimethyl fumarate DMIdimethyl itaconate DMPDess-Martin periodinane DMSOdimethyl sulfoxide EDC1-ethyl-3-(3-dimethylaminopropyl)carbodiimide EDTAethylenediaminetetraacetic acid EGPAeosinophilic granulomatosis with polyangiitis EtOAcethyl acetate FBSfetal bovine serum FCASfamilial cold autoinflammatory syndrome FMFfamilial Mediterranean fever GAPDHglyceraldehyde 3-phosphate dehydrogenase GPAgranulomatosis with polyangiitis GSHglutathione GVHDgraft versus host disease HATU1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate HCA2hydroxycarboxylic acid receptor 2 HCVhepatitis C HFChydrofluorocarbon HIF-1αhypoxia-inducible factor-1α HIVhuman immunodeficiency virus HMDMshuman monocyte derived macrophages HOBt1-hydroxybenzotriazole ILinterleukin IPEXimmune dysregulation polyendocrinopathy enteropathy X-linked IRG1immune-responsive gene 1 IVIGintravenous immune globulin JAKJanus kinase KEAP1kelch-like ECH-associated protein 1 LCMSliquid chromatography-mass spectrometry LDAlithium diisopropylamide LPSlipopolysaccharide MALTmucosa-associated lymphoid tissue mCPBAmeta-chloroperoxybenzoic acid M-CSFmacrophage-colony stimulating factor MMFmonomethyl fumarate MMNmultifocal motor neuropathy MOGmyelin oligodendrocyte glycoprotein MSmass spectrometry MSDmass selective detector MTBEmethyl tertiary-butyl ether NLRP12ADNLRP12-associated autoinflammatory disorders NMMN-methylmorpholine NMRnuclear magnetic resonance NOMIDneonatal onset multisystem inflammatory disease NRF2nuclear factor (erythroid-derived 2)-like 2 NSAIDsnon-steroidal anti-inflammatory drugs O / Novernight PAHpulmonary arterial hypertension PANDASpaediatric autoimmune neuropsychiatric disorders associated with streptococcal infections PANSpaediatric acute-onset neuropsychiatric syndrome PAPApyogenic arthritis, pyoderma gangrenosum, severe cystic acne PMB4-methoxybenzyl PBMCsprimary peripheral blood mononuclear cells PBSphosphate buffered saline PDAphotodiode array PDE4phosphodiesterase-4 PETpositron emission topography PFAPAperiodic fever aphthous stomatitis PLEVApityriasis lichenoides et varioliformis acuta PMAphorbol 12-myristate 13-acetate PSCprimary sclerosing cholangitis PUVApsoralen ultraviolet irradiation p-TsOHp-toluenesulfonic acid 4OI4-octyl itaconic acid RTroom temperature sat.saturated SDHsuccinate dehydrogenase SFCsupercritical fluid chromatography SLEsystemic lupus erythematosus STINGstimulator of interferon genes TFAtrifluoroacetic acid TLRToll-like receptor TNFtumour necrosis factor TRAPStumour necrosis factor receptor-associated periodic fever Trttrityl, triphenylmethyl TTPthrombotic thrombocytopenic purpura UPLCultra performance liquid chromatography VEGFvascular endothelial growth factor VWDvariable wavelength detector wt.weight EXAMPLES Analytical Equipment

[0172] NMR spectra were recorded using a Bruker 400 MHz Avance III spectrometer fitted with a BBFO 5 mm probe, or a Bruker 500 MHz Avance III HD spectrometer equipped with a Bruker 5 mm SmartProbeTM. Spectra were measured at 298 K, unless indicated otherwise, and were referenced relative to the solvent resonance. The chemical shifts are reported in parts per million. Data were acquired using Bruker TopSpin software.

[0173] UPLC / MS analysis was carried out on a Waters Acquity UPLC system using either a Waters Acquity CSH C18 or BEH C18 column (2.1 x 30 mm) maintained at a temperature of 40 °C and eluted with a linear acetonitrile gradient appropriate for the lipophilicity of the compound over 3 or 10 minutes at a constant flow rate of 0.77 mL / min. The aqueous portion of the mobile phase was either 0.1 % Formic Acid (CSH C18 column) or 10 mM Ammonium Bicarbonate (BEH C18 column). LC-UV chromatograms were recorded using a Waters Acquity PDA detector between 210 and 400 nm. Mass spectra were recorded using a Waters Acquity Qda detector with electrospray ionisation switching between positive and negative ion mode. Sample concentration was adjusted to give adequate UV response.

[0174] LCMS analysis was carried out on a Agilent LCMS system using either a Waters Acquity CSH C18 or BEH C18 column (4.6 x 30 mm) maintained at a temperature of 40 °C and eluted with a linear acetonitrile gradient appropriate for the lipophilicity of the compound over 4 or 15 minutes at a constant flow rate of 2.5 mL / min. The aqueous portion of the mobile phase was either 0.1 % Formic Acid (CSH C18 column) or 10 mM Ammonium Bicarbonate (BEH C18 column). LC-UV chromatograms were recorded using an Agilent VWD or DAD detector at 254 nm. Mass spectra were recorded using an Agilent MSD detector with electrospray ionisation switching between positive and negative ion mode. Sample concentration was adjusted to give adequate UV response.

[0175] Alternatively, the following analytical LCMS equipment and methods were also used: LCMS / HPLC Instrument Details System Instrument Name LC Detector ELS detector Mass detector 1 Agilent LCMS 1200G1315D DAD380 ELSDAgilent G6120B2 Agilent LCMS 1200G1315C DAD380 ELSDAgilent G6110A LCMS / HPLC Method Details Method Name Solvent System Column Gradient UV range Mass Range Column Temp. °C Flow Rate ml / min A A) water + 10 mM NH 4 HCO 3 Waters X-Bridge C18 (50 mm x 4.6 mm x 3.5 µm)From 95:5 to 0:100 in 1.6 min, 0:100 for 1.4 min, from 0:100 to 95:5 in 0.1 min, 95:5 for 0.7 min190-400 nm100-1800 amu402.0B) acetonitrileB A) water + 0.05% TFAWaters X-Bridge C18 (50 mm x 4.6 mm x 3.5 µm)From 95:5 to 0:100 in 1.6 min, 0:100 for 1.4 min, from 0:100 to 95:5 in 0.05 min, 95:5 for 0.7 min190-400 nm100-1100 amu402.0B) acetonitrile + 0.05% TFAC A) water + 0.05% TFAHalo C18 (30 mm x 4.6 mm x 2.7 µm)From 95:5 to 0:100 in 0.8 min, 0:100 for 0.4 min, from 0:100 to 95:5 in 0.01 min, 95:5 for 0.2 min190-400 nm100-1100 amu403.0 Commercial Materials

[0176] Dimethyl itaconate was purchased from Sigma-Aldrich (product number: 109533); 4-octyl itaconate was purchased from BOC biosciences (product number: B0001-007866); 4-methyl itaconate was purchased from Apollo Scientific (product number: OR10969); 4-butyl itaconate was purchased from Combi-Blocks (product number: QV-5962).General Methods

[0177] Unless otherwise stated all reactions were stirred. Organic solutions were routinely dried over anhydrous magnesium sulfate. Hydrogenations were performed on a Thales H-cube flow reactor under the conditions stated or under pressure in a gas autoclave (bomb).General Procedure 1 - synthesis of monoesters

[0178]

[0179] A suspension of itaconic anhydride (1 eq.) in toluene was treated with p-TsOH.H 2 O (10 mol%) followed by the appropriate alcohol (R-OH, 1 eq.) which is defined where relevant below. The resultant yellow solution was stirred at 80-110 °C. The reaction mixture was concentrated onto silica gel and the crude product was purified by chromatography on silica gel (0-5% MeOH / DCM) to afford the desired monoester.General Procedure 2 - synthesis of diesters

[0180]

[0181] A solution of the appropriate itaconic acid monoester (1 eq.) which is defined where relevant below, EDC.HCl (1.5 eq.) and DMAP (5-200 mol%) in DCM was treated with DIPEA (3 eq.) and the appropriate alcohol (R 2< -OH, 1.1 eq.) which is defined where relevant below. The resulting solution was stirred at RT for 20 h. The reaction mixture was concentrated onto silica and the crude product purified by chromatography on silica gel (0-5% MeOH / DCM) to afford the desired diester.General Procedure 3 - synthesis of diesters

[0182]

[0183] A solution of the appropriate alcohol (R 2< -OH, 1 eq.) which is defined where relevant below, HOBt.H 2 O hydrate (2 eq.) and EDC.HCl (2 eq.) in DCM was treated with the appropriate itaconic acid monoester (1 eq.) which is defined where relevant below, followed by dropwise addition of DIPEA (3 eq.) at RT. The resulting mixture was stirred at RT for 16 h. The reaction mixture was concentrated in vacuo and purified by column chromatography on silica gel (DCM / EtOAc 1:1) to provide the desired diester.General Procedure 4 - synthesis of diesters

[0184]

[0185] A mixture of the appropriate itaconic acid monoester (1 eq.) which is defined where relevant below, potassium carbonate (1.2 eq.) and iodomethane (1.2 eq.) in acetone was stirred for 18 h at RT. The mixture was filtered and the filtrate was concentrated onto silica gel. The crude product was purified by chromatography on silica gel (0-20% EtOAc / isohexane) to afford the desired diester.

[0186] In any one of the above General Procedures, suitably R 1< is R A< ' which is defined elsewhere herein.Intermediate 1 - 4-(cyclooctyloxy)-2-methylene-4-oxobutanoic acid

[0187]

[0188] Intermediate 1 was prepared according to General Procedure 1, using cyclooctanol as R-OH. 1< H NMR (500 MHz, DMSO-d6) δ 12.59 (br. s, 1H), 6.13 (d, J = 1.6 Hz, 1H), 5.75 - 5.72 (m, 1H), 4.82 (tt, J = 8.2, 3.9 Hz, 1H), 3.25 (s, 2H), 1.76 - 1.40 (m, 14H). LCMS m / z 263.2 (M+Na) +< (ES +< ); 239.2 (M-H) -< (ES -< ).Intermediate 2 - 2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoic acid

[0189]

[0190] Intermediate 2 was prepared according to General Procedure 1, using 3-phenoxypropan-1-ol as R-OH. 1< H NMR (500 MHz, DMSO-d6) δ 12.65 (br. s, 1H), 7.32 - 7.26 (m, 2H), 6.95 - 6.91 (m, 3H), 6.15 (d, J = 1.6 Hz, 1H), 5.76 (br. s, 2H), 4.18 (t, J = 6.4 Hz, 2H), 4.02 (t, J = 6.3 Hz, 2H), 3.33 (br. s, 4H), 2.01 (qu, J = 6.3 Hz, 2H). LCMS m / z 287.1 (M+Na) +< (ES +< ); 263.1 (M-H) -< (ES -< ).Intermediate 3 - 4-(hexyloxy)-2-methylene-4-oxobutanoic acid

[0191]

[0192] Intermediate 3 was prepared according to General Procedure 1, using hexan-1-ol as R-OH. 1< H NMR (500 MHz, DMSO-d6) δ 12.57 (br. s, 1H), 6.15 (d, J = 1.6 Hz, 1H), 5.78 - 5.74 (m, 1H), 4.01 (t, J = 6.6 Hz, 2H), 3.30 (s, 2H), 1.58 - 1.51 (m, 2H), 1.35 - 1.21 (m, 6H), 0.87 (t, J = 6.8 Hz, 3H). LCMS m / z 237.2 (M+H) +< (ES +< ); 213.2 (M-H) -< (ES -< ).Intermediate 4 - 4-(cyclohexyloxy)-2-methylene-4-oxobutanoic acid

[0193]

[0194] Intermediate 4 was prepared according to General Procedure 1, using cyclohexanol as R-OH. 1< H NMR (500 MHz, CDCl 3 ) δ 6.44 (d, J = 0.9 Hz, 1H), 5.81 (d, J = 1.2 Hz, 1H), 4.90 - 4.69 (m, 1H), 3.32 (s, 2H), 1.83 - 1.64 (m, 4H), 1.55 - 1.16 (m, 6H). 1< H NMR (500 MHz, DMSO-d6) δ 12.62 (s, 1H), 6.17 (d, J = 1.6 Hz, 1H), 5.78 (q, J = 1.3 Hz, 1H), 4.15 - 4.11 (m, 2H), 3.61 - 3.58 (m, 2H), 3.54 - 3.51 (m, 2H), 3.49 - 3.46 (m, 2H), 3.43 (q, J = 7.0 Hz, 2H), 3.33 (s, 2H), 1.10 (t, J = 7.0 Hz, 3H). LCMS m / z 210.7 (M-H) -< (ES -< ).Intermediate 5 - 4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoic acid

[0195]

[0196] Intermediate 5 was prepared according to General Procedure 1, using (4-fluorophenyl)methanol as R-OH. 1< H NMR (500 MHz, DMSO-d6) δ 12.67 (s, 1H), 7.44 - 7.38 (m, 2H), 7.23 - 7.16 (m, 2H), 6.17 (d, J = 1.6 Hz, 1H), 5.81 - 5.77 (m, 1H), 5.09 (s, 2H), 3.38 (s, 2H). LCMS m / z 237.3 (M-H) -< (ES -< ).Intermediate 6 - (R)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid

[0197]

[0198] Intermediate 6 was prepared according to General Procedure 1, using (R)-octan-2-ol as R-OH. 1< H NMR (500 MHz, CDCl 3 ) δ 12.58 (s, 1H), 6.13 (d, J = 1.6 Hz, 1H), 5.74 (d, J = 1.6 Hz, 1H), 4.88 - 4.58 (m, 1H), 3.26 (s, 2H), 1.54 - 1.38 (m, 2H), 1.30 - 1.20 (m, 8H), 1.13 (d, J = 6.2 Hz, 3H), 0.85 (t, J = 6.7 Hz, 3H). LCMS m / z 241.2 (M-H) -< (ES -< ).Intermediate 7 - (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid

[0199]

[0200] Intermediate 7 was prepared according to General Procedure 1, using (S)-octan-2-ol as R-OH. 1< H NMR (500 MHz, DMSO-d6) δ 12.58 (s, 1H), 6.14 (d, J = 1.6 Hz, 1H), 5.75 (d, J = 1.6 Hz, 1H), 4.84 - 4.74 (m, 1H), 3.26 (s, 2H), 1.56 - 1.40 (m, 2H), 1.33 - 1.20 (m, 8H), 1.14 (d, J = 6.2 Hz, 3H), 0.86 (t, J = 6.8 Hz, 3H). LCMS m / z 241.0 (M-H) -< (ES -< ).Intermediate 8 - 2-methylene-4-(neopentyloxy)-4-oxobutanoic acid

[0201]

[0202] Intermediate 8 was prepared according to General Procedure 1, using 2,2-dimethylpropan-1-ol as R-OH. 1< H NMR (500 MHz, DMSO-d6) δ 12.63 (s, 1H), 6.16 (d, J = 1.6 Hz, 1H), 5.77 (d, J = 1.4 Hz, 1H), 3.72 (s, 2H), 3.34 (s, 2H), 0.88 (s, 9H). LCMS m / z 198.9 (M-H) -< (ES -< ).Intermediate 9 - 3-((2-(tert-butoxy)-2-oxoethoxy)carbonyl)but-3-enoic acid

[0203] Step 1

[0204] Boron trifluoride diethyl etherate (1.43 mL, 11.6 mmol) was added to a mixture of itaconic anhydride (10 g, 89 mmol) and 2,2,2-trichloroethanol (15.4 mL, 161 mmol) under nitrogen at RT. The reaction mixture was heated to 95 °C for 30 mins, then cooled to RT. The residue was treated with sat. aq. NaHCO 3 (400 mL) and washed with EtOAc (3 x 100 mL). The aqueous phase was acidified to pH=2 with concentrated HCl and extracted with EtOAc (3 x 120 mL). The combined organic layers were dried (MgSO 4 ) and concentrated. The residue was recrystallised from a mixture of toluene and iso-hexane (1:1) (300 mL). The resulting solid was filtered, washed with iso-hexane and dried in vacuo to afford 2-methylene-4-oxo-4-(2,2,2-trichloroethoxy)butanoic acid (13.7 g, 51.3 mmol) as a white crystalline solid. 1< H NMR (400 MHz, DMSO-d6) δ 12.70 (s, 1H), 6.21 (d, J = 1.5 Hz, 1H), 5.86 (d, J = 1.3 Hz, 1H), 4.88 (s, 2H), 3.49 (s, 2H).Step 2

[0205] Potassium carbonate (4.16 g, 30.1 mmol) was added portionwise to a solution of 2-methylene-4-oxo-4-(2,2,2-trichloroethoxy)butanoic acid (7.50 g, 28.7 mmol) in acetone (140 mL) at RT. After 5 min tert-butyl bromoacetate (4.45 mL, 30.1 mmol) was added dropwise. The reaction mixture was stirred at RT for 16 h, then diluted with EtOAc (150 mL) and filtered. The filtrate was concentrated to afford 1-(2-(tert-butoxy)-2-oxoethyl) 4-(2,2,2-trichloroethyl) 2-methylenesuccinate (10.7 g, 28.5 mmol) as a white solid. 1< H NMR (400 MHz, DMSO-d6) δ 6.36 (d, J = 1.2 Hz, 1H), 6.03 (q, J = 1.1 Hz, 1H), 4.89 (s, 2H), 4.62 (s, 2H), 3.58 (d, J = 1.0 Hz, 2H), 1.42 (s, 9H).Step 3

[0206] Zinc (11.2 g, 171 mmol) was added portionwise over 5 min to a solution of 1-(2-(tert-butoxy)-2-oxoethyl) 4-(2,2,2-trichloroethyl) 2-methylenesuccinate (10.7 g, 28.5 mmol) in acetic acid (160 mL). The reaction mixture was stirred at RT for 18 h then diluted with water (100 mL) and EtOAc (300 mL). The mixture was carefully decanted and the phases were separated. The aqueous phase was extracted with EtOAc (3 x 200 mL). The combined organic extracts were washed with brine (3 x 150 mL), dried (Na 2 SO 4 ) and concentrated to afford the title compound as a colourless solid (6.07 g). An analytically pure sample was obtained by recrystallisation of a small sample (300 mg) from toluene and isohexane (1:1). 1< H NMR (400 MHz, DMSO-d6) δ 12.35 (s, 1H), 6.26 (d, J = 1.3 Hz, 1H), 5.91 - 5.85 (m, 1H), 4.62 (s, 2H), 3.32 (s, 2H), 1.42 (s, 9H). LCMS m / z 267.1 (M+Na) +< (ES +< ).Example 1 - 2-((2-methylene-4-(octyloxy)-4-oxobutanoyl)oxy)acetic acid

[0207] Step 1

[0208] tert-Butyl bromoacetate (0.37 mL, 2.5 mmol) was added to a mixture of 4-octyl itaconate (0.50 g, 2.1 mmol) and potassium carbonate (0.35 g, 2.5 mmol) in acetone (10 mL). The mixture was stirred for 16 h at RT. The mixture was diluted with EtOAc (20 mL), filtered and concentrated onto silica gel. The crude product was purified by chromatography on silica gel (0-30% EtOAc / isohexane) to afford 1-(2-(tert-butoxy)-2-oxoethyl) 4-octyl 2-methylenesuccinate as a colourless oil. 1< H NMR (500 MHz, DMSO-d6) δ 6.29 (d, J = 1.3 Hz, 1H), 5.95 - 5.92 (m, 1H), 4.61 (s, 2H), 4.01 (t, J = 6.6 Hz, 2H), 3.38 (s, 2H), 1.60 - 1.50 (m, 2H), 1.42 (s, 9H), 1.34 - 1.21 (m, 10H), 0.90 - 0.83 (m, 3H). LCMS m / z 379.3 (M+Na) +< (ES +< ).Step 2

[0209] TFA (5 mL, 65 mmol) was added to a solution of 1-(2-(tert-butoxy)-2-oxoethyl) 4-octyl 2-methylenesuccinate (0.65 g, 1.82 mmol) in DCM (5 mL). The reaction mixture was stirred for 30 minutes, diluted with toluene (10 mL) and concentrated. The crude product was purified by chromatography on silica gel (0-4% MeOH / DCM) to afford the title compound as a colourless oil. 1< H NMR (500 MHz, DMSO-d6) δ 13.08 (s, 1H), 6.29 (d, J = 1.2 Hz, 1H), 5.93 (q, J = 1.2 Hz, 1H), 4.64 (s, 2H), 4.01 (t, J = 6.6 Hz, 2H), 3.38 (s, 2H), 1.60 - 1.48 (m, 2H), 1.32 - 1.20 (m, 10H), 0.92 - 0.82 (m, 3H). LCMS m / z 323.2 (M+Na) +< (ES +< ).Example 2 - 2-((2-methylene-4-(octyloxy)-4-oxobutanoyl)oxy)propanoic acid

[0210] Step 1

[0211] tert-Butyl 2-bromopropanoate (1.03 g, 4.95 mmol) was added to a suspension of 4-octyl itaconate (1.00 g, 4.13 mmol) and potassium carbonate (0.696 g, 5.03 mmol) in acetone (20 mL). The reaction mixture was stirred at RT for 18 h, then heated to 50 °C and stirred for a further 5 h. The reaction mixture was diluted with ethyl acetate (40 mL), filtered and concentrated to afford 1-(1-(tert-butoxy)-1-oxopropan-2-yl) 4-octyl 2-methylenesuccinate (1.7 g, 3.07 mmol). 1< H NMR (500 MHz, DMSO-d6) δ 6.27 (d, J = 1.3 Hz, 1H), 5.95 - 5.88 (m, 1H), 4.88 (q, J = 7.0 Hz, 1H), 4.00 (t, J = 6.6 Hz, 2H), 3.41 - 3.32 (m, 2H), 1.59 - 1.51 (m, 2H), 1.43 - 1.35 (m, 12H), 1.32 - 1.18 (m, 10H), 0.89 - 0.84 (m, 3H). LCMS m / z 393.2 (M+Na) +< (ES +< ).Step 2

[0212] TFA (11 mL) was added to a solution of 1-(1-(tert-butoxy)-1-oxopropan-2-yl) 4-octyl 2-methylenesuccinate (1.53 g, 4.13 mmol) in DCM (11 mL) and the mixture was stirred for 30 min at RT. The reaction was diluted with toluene (20 mL) and concentrated. The residue was dissolved in ethyl acetate (40 mL) and washed with water (10 x 25 mL). The organic phase was dried (MgSO 4 ) and concentrated to afford the title compound (1.00 g, 3.15 mmol) as a colourless oil. 1< H NMR (500 MHz, DMSO-d6) δ 13.06 (s, 1H), 6.27 (d, J = 1.3 Hz, 1H), 5.90 (d, J = 1.3 Hz, 1H), 4.96 (q, J = 7.1 Hz, 1H), 4.00 (t, J = 6.6 Hz, 2H), 3.36 (s, 2H), 1.59 - 1.50 (m, 2H), 1.42 (d, J = 7.0 Hz, 3H), 1.31 - 1.22 (m, 10H), 0.86 (t, J = 6.8 Hz, 3H). LCMS m / z 337.2 (M+Na) +< (ES +< ).Example 3 - 3-((2-methylene-4-(octyloxy)-4-oxobutanoyl)oxy)propanoic acid

[0213] Step 1

[0214] A solution of 4-octyl itaconate (2.00 g, 8.25 mmol), tert-butyl 3-hydroxypropanoate (1.49 mL, 9.90 mmol), N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (3.16 g, 16.5 mmol) and DMAP (0.101 g, 0.83 mmol) in DCM (60 mL) was treated with DIPEA (2.88 mL, 16.5 mmol). The resulting solution was stirred at RT for 20 h. The reaction mixture was concentrated onto silica gel and the crude product purified by chromatography on silica gel (0-20% EtOAc / DCM or 0-20% EtOAc / DCM) to afford 1-(3-(tert-butoxy)-3-oxopropyl) 4-octyl 2-methylenesuccinate (1.56 g, 4.13 mmol) as a clear colourless oil. 1< H NMR (500 MHz, DMSO-d6) δ 6.30 (d, J = 1.0 Hz, 1H), 5.70 (d, J = 1.2 Hz, 1H), 4.39 (t, J = 6.5 Hz, 2H), 4.08 (t, J = 6.8 Hz, 2H), 3.32 (s, 2H), 2.59 (t, J = 6.5 Hz, 2H), 1.63 - 1.51 (m, 2H), 1.45 (s, 9H), 1.32 - 1.13 (m, 10H), 0.88 (t, J = 6.9 Hz, 3H). LCMS m / z 392.9 (M+Na) +< (ES +< ).Step 2

[0215] TFA (15 mL) was added to a solution of 1-(3-(tert-butoxy)-3-oxopropyl) 4-octyl 2-methylenesuccinate (1.56 g, 4.21 mmol) in DCM (15 mL) and the mixture was stirred for 2 h at RT. The reaction was diluted with toluene (50 mL) and concentrated. The residue was co-evaporated with toluene (2 x 20 mL) and dried in vacuo to afford the title compound (1.546 g, 4.87 mmol) as a colourless solid. 1< H NMR (500 MHz, DMSO-d6) δ 12.37 (s, 1H), 6.16 (d, J = 1.3 Hz, 1H), 5.82 (d, J = 1.4 Hz, 1H), 4.25 (t, J = 6.2 Hz, 2H), 4.00 (t, J = 6.6 Hz, 2H), 3.32 (s, 2H), 2.59 (t, J = 6.2 Hz, 2H), 1.58 - 1.47 (m, 2H), 1.36 - 1.16 (m, 10H), 0.86 (t, J = 6.8 Hz, 3H). LCMS m / z 337.2 (M+Na) +< (ES +< ).Example 4 - 3-((4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid

[0216]

[0217] Example 4 was prepared according to the procedure of Example 3, but using 4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoic acid (Intermediate 5) instead of 4-octyl itaconate. 1< H NMR (500 MHz, DMSO-d6) δ 12.39 (br s, 1H), 7.44 - 7.38 (m, 2H), 7.25 - 7.17 (m, 2H), 6.18 (d, J = 1.3 Hz, 1H), 5.85 (d, J = 1.3 Hz, 1H), 5.08 (s, 2H), 4.24 (t, J = 6.2 Hz, 2H), 3.41 (s, 2H), 2.57 (t, J = 6.2 Hz, 2H). LCMS m / z 333.3 (M+Na) +< (ES +< ).Example 5 - 3-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid

[0218]

[0219] Example 5 was prepared according to the procedure of Example 3, but using 4-(cyclooctyloxy)-2-methylene-4-oxobutanoic acid (Intermediate 1) instead of 4-octyl itaconate. 1< H NMR (500 MHz, DMSO-d6) δ 12.39 (s, 1H), 6.15 (d, J = 1.4 Hz, 1H), 5.85 - 5.74 (m, 1H), 4.83 (tt, J = 8.1, 3.9 Hz, 1H), 4.26 (t, J = 6.2 Hz, 2H), 3.30 (s, 2H), 2.60 (t, J = 6.2 Hz, 2H), 1.77 - 1.41 (m, 14H). LCMS m / z 335.3 (M+Na) +< (ES +< ).Example 6 - 3-((2-methylene-4-(neopentyloxy)-4-oxobutanoyl)oxy)propanoic acid

[0220]

[0221] Example 6 was prepared according to the procedure of Example 3, but using 2-methylene-4-(neopentyloxy)-4-oxobutanoic acid (Intermediate 8) instead of 4-octyl itaconate. 1< H NMR (400 MHz, DMSO-d6) δ 12.38 (s, 1H), 6.18 (d, J = 1.4 Hz, 1H), 5.88 - 5.82 (m, 1H), 4.26 (t, J = 6.2 Hz, 2H), 3.72 (s, 2H), 3.38 (s, 2H), 2.59 (t, J = 6.2 Hz, 2H), 0.88 (s, 9H). LCMS m / z 295.7 (M+Na) +< (ES +< ).Example 7 - (S)-3-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)propanoic acid

[0222]

[0223] Example 7 was prepared according to the procedure of Example 3, but using S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid (Intermediate 7) instead of 4-octyl itaconate. 1< H NMR (400 MHz, DMSO-d6) δ 12.39 (s, 1H), 6.15 (d, J = 1.3 Hz, 1H), 5.81 (d, J = 1.3 Hz, 1H), 4.84 - 4.70 (m, 1H), 4.25 (t, J = 6.2 Hz, 2H), 3.30 (s, 2H), 2.59 (t, J = 6.2 Hz, 2H), 1.54 - 1.37 (m, 2H), 1.25 (dd, J = 10.7, 5.2 Hz, 8H), 1.13 (d, J = 6.2 Hz, 3H), 0.85 (t, J = 6.4 Hz, 3H). LCMS m / z 336.9 (M+Na) +< (ES +< ).Example 8 - 3-((4-(hexyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid

[0224]

[0225] Example 8 was prepared according to the procedure of Example 3, but using 4-(hexyloxy)-2-methylene-4-oxobutanoic acid (Intermediate 3) instead of 4-octyl itaconate. 1< H NMR (400 MHz, DMSO-d6) δ 12.39 (s, 1H), 6.16 (d, J = 1.4 Hz, 1H), 5.82 (d, J = 1.3 Hz, 1H), 4.25 (t, J = 6.2 Hz, 2H), 4.00 (t, J = 6.6 Hz, 2H), 3.33 (s, 2H), 2.59 (t, J = 6.2 Hz, 2H), 1.61 - 1.47 (m, 2H), 1.32 - 1.19 (m, 6H), 0.93 - 0.78 (m, 3H). LCMS m / z 309.7 (M+Na) +< (ES +< ).Example 9 - 3-((2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoyl)oxy)propanoic acid

[0226]

[0227] Example 9 was prepared according to the procedure of Example 3, but using 2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoic acid (Intermediate 2) instead of 4-octyl itaconate. 1< H NMR (400 MHz, DMSO-d6) δ 12.41 (s, 1H), 7.35 - 7.24 (m, 2H), 6.99 - 6.88 (m, 3H), 6.17 (d, J = 1.3 Hz, 1H), 5.83 (q, J = 1.2 Hz, 1H), 4.24 (t, J = 6.2 Hz, 2H), 4.19 (t, J = 6.4 Hz, 2H), 4.01 (t, J = 6.3 Hz, 2H), 3.37 (s, 2H), 2.58 (t, J = 6.2 Hz, 2H), 2.06 - 1.97 (m, 2H). LCMS m / z 359.3 (M+Na) +< (ES +< ).Example 10 - 3-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid

[0228]

[0229] Example 10 was prepared according to the procedure of Example 3, but using 4-(cyclohexyloxy)-2-methylene-4-oxobutanoic acid (Intermediate 4) instead of 4-octyl itaconate. 1< H NMR (400 MHz, DMSO-d6) δ 12.40 (s, 1H), 6.15 (d, J = 1.4 Hz, 1H), 5.81 (d, J = 1.3 Hz, 1H), 4.70 - 4.59 (m, 1H), 4.25 (t, J = 6.2 Hz, 2H), 3.31 (s, 2H), 2.59 (t, J = 6.2 Hz, 2H), 1.77 - 1.70 (m, 2H), 1.65 - 1.55 (m, 2H), 1.49 - 1.16 (m, 6H). LCMS m / z 307.4 (M+Na) +< (ES +< ).Example 11 - (R)-3-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)propanoic acid

[0230]

[0231] Example 11 was prepared according to the procedure of Example 3, but using (R)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid (Intermediate 6) instead of 4-octyl itaconate. 1< H NMR (400 MHz, DMSO-d6) δ 12.38 (s, 1H), 6.15 (d, J = 1.4 Hz, 1H), 5.81 (d, J = 1.4 Hz, 1H), 4.83 - 4.72 (m, 1H), 4.25 (t, J = 6.2 Hz, 2H), 3.30 (s, 2H), 2.59 (t, J = 6.3 Hz, 2H), 1.54 - 1.39 (m, 2H), 1.29 - 1.16 (m, 8H), 1.13 (d, J = 6.3 Hz, 3H), 0.85 (t, J = 6.4 Hz, 3H). LCMS m / z 337.4 (M+Na) +< (ES +< ).Example 12 - (S)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid

[0232] Step 1

[0233] A mixture of (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid (Intermediate 7, 1 g, 4.13 mmol), tert-butyl bromoacetate (0.64 mL, 4.3 mmol) and potassium carbonate (0.684 g, 4.95 mmol) in acetone (20 mL) was stirred at RT overnight. The reaction mixture was concentrated and the residue was partitioned between water (20 mL) and EtOAc (10 mL). The phases were separated and the aqueous phase was extracted with EtOAc (10 mL). The combined organic phases were washed with brine (10 mL), dried (MgSO 4 ) and concentrated to afford (S)-1-(2-(tert-butoxy)-2-oxoethyl) 4-octan-2-yl 2-methylenesuccinate (1.56 g, 4.07 mmol) as a colourless oil. 1< H NMR (500 MHz, DMSO-d6) δ 6.28 (s, 1H), 5.92 (s, 1H), 4.82 - 4.75 (m, 1H), 4.60 (s, 2H), 3.35 (s, 2H), 1.42 (s, 9H), 1.31 - 1.20 (m, 10H), 1.14 (d, J = 6.3 Hz, 3H), 0.88 - 0.84 (m, 3H).Step 2

[0234] TFA (7.5 mL) was added to a solution of (S)-1-(2-(tert-butoxy)-2-oxoethyl) 4-octan-2-yl 2-methylenesuccinate (1.5 g, 3.79 mmol) in DCM (7.5 mL). The reaction mixture was stirred for 2 h, partitioned between EtOAc (50 mL) and water (50 mL) and the phases separated. The organic phase was washed with water (2 x 20 mL), brine (20 mL), dried (MgSO 4 ) and concentrated. The residue was redissolved in DCM (10 mL), washed with water (2 x 10 mL), dried (MgSO 4 ) and concentrated to afford the title compound (0.922 g, 3.04 mmol) as a colourless oil. 1< H NMR (500 MHz, DMSO-d6) δ 13.08 (br. s, 1H), 6.28 (d, J = 1.2 Hz, 1H), 5.92 (d, J = 1.3 Hz, 1H), 4.83 - 4.74 (m, 1H), 4.64 (s, 2H), 3.35 (s, 2H), 1.55 - 1.40 (m, 2H), 1.31 - 1.18 (m, 8H), 1.14 (d, J = 6.2 Hz, 3H), 0.86 (t, J = 6.9 Hz, 3H). LCMS m / z 323.2 (M+H) +< (ES +< ).Example 13 - 2-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0235]

[0236] Example 13 was prepared according to the procedure of Example 12, but using 4-(cyclooctyloxy)-2-methylene-4-oxobutanoic acid (Intermediate 1) instead of (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid. 1< H NMR (500 MHz, DMSO-d6) δ 13.11 (s, 1H), 6.27 (d, J = 1.2 Hz, 1H), 5.91 (d, J = 1.4 Hz, 1H), 4.82 (tt, J = 8.1, 3.9 Hz, 1H), 4.63 (s, 2H), 3.33 (s, 2H), 1.75 - 1.42 (m, 14H). LCMS m / z 321.3 (M+Na) +< (ES +< ).Example 14 - 2-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0237]

[0238] Example 14 was prepared according to the procedure of Example 12, but using 4-(cyclohexyloxy)-2-methylene-4-oxobutanoic acid (Intermediate 4) instead of (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid. 1< H NMR (500 MHz, DMSO-d6) δ 13.09 (s, 1H), 6.27 (d, J = 1.3 Hz, 1H), 5.91 (d, J = 1.3 Hz, 1H), 4.68 - 4.59 (m, 3H), 3.35 (s, 2H), 1.77 - 1.70 (m, 2H), 1.65 - 1.58 (m, 2H), 1.50 - 1.17 (m, 6H). LCMS m / z 293.6 (M+Na) +< (ES +< ).Example 15 - 2-((2-methylene-4-(neopentyloxy)-4-oxobutanoyl)oxy)acetic acid

[0239]

[0240] Example 15 was prepared according to the procedure of Example 12, but using 2-methylene-4-(neopentyloxy)-4-oxobutanoic acid (Intermediate 8) instead of (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid. 1< H NMR (500 MHz, DMSO-d6) δ 13.08 (s, 1H), 6.30 (d, J = 1.2 Hz, 1H), 5.95 (d, J = 1.2 Hz, 1H), 4.64 (s, 2H), 3.73 (s, 2H), 3.42 (s, 2H), 0.88 (s, 9H). LCMS m / z 280.7 (M+Na) +< (ES +< ).Example 16 - 2-((4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0241]

[0242] Example 16 was prepared according to the procedure of Example 12, but using 4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoic acid (Intermediate 5) instead of (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid. 1< H NMR (400 MHz, DMSO-d6) δ 13.10 (br. s, 1H), 7.44 - 7.37 (m, 2H), 7.25 - 7.17 (m, 2H), 6.30 (d, J = 1.2 Hz, 1H), 5.95 (d, J = 1.2 Hz, 1H), 5.09 (s, 2H), 4.63 (s, 2H), 3.45 (s, 2H). LCMS m / z 297.3 (M+H) +< (ES +< ).Example 17 - 2-((4-(hexyloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0243]

[0244] Example 17 was prepared according to the procedure of Example 12, but using 4-(hexyloxy)-2-methylene-4-oxobutanoic acid (Intermediate 3) instead of (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid. 1< H NMR (400 MHz, DMSO-d6) δ 13.10 (s, 1H), 6.28 (d, J = 1.2 Hz, 1H), 5.92 (d, J = 1.2 Hz, 1H), 4.63 (s, 2H), 4.00 (t, J = 6.6 Hz, 2H), 3.37 (s, 2H), 1.58 - 1.42 (m, 2H), 1.31 - 1.11 (m, 6H), 0.86 (t, J = 6.8 Hz, 3H). LCMS m / z 295.3 (M+Na) +< (ES +< ).Example 18 - 2-((2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoyl)oxy)acetic acid

[0245]

[0246] Example 18 was prepared according to the procedure of Example 12, but using 2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoic acid (Intermediate 2) instead of (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid. 1< H NMR (400 MHz, DMSO-d6) δ 13.09 (s, 1H), 7.33 - 7.25 (m, 2H), 6.98 - 6.90 (m, 3H), 6.29 (d, J = 1.2 Hz, 1H), 5.97 - 5.90 (m, 1H), 4.62 (s, 2H), 4.19 (t, J = 6.4 Hz, 2H), 4.01 (t, J = 6.3 Hz, 2H), 3.41 (s, 2H), 2.07 - 1.97 (m, 2H). LCMS m / z 345.3 (M+Na) +< (ES +< ).Example 19 - 2-((2-methylene-4-oxo-4-(spiro[3.3]heptan-2-yloxy)butanoyl)oxy)acetic acid

[0247] Step 1

[0248] N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (0.471 g, 2.46 mmol) was added to a solution of 3-((2-(tert-butoxy)-2-oxoethoxy)carbonyl)but-3-enoic acid (Intermediate 9, 0.400 g, 1.64 mmol), spiro[3.3]heptan-2-ol (0.220 g, 1.97 mmol) and DMAP (0.300 g, 2.46 mmol) in DCM (10 mL). The reaction mixture was stirred at RT for 18 h. The reaction mixture was concentrated and the crude product was purified by chromatography on silica gel (0-10% EtOAc / DCM) to afford impure product. The crude product was purified by chromatography on silica gel (0-10% EtOAc / isohexane) to afford 1-(2-(tert-butoxy)-2-oxoethyl) 4-spiro[3.3]heptan-2-yl 2-methylenesuccinate (146 mg, 0.41 mmol) as a pale yellow oil.Step 2

[0249] TFA (1 mL) was added to a solution of 1-(2-(tert-butoxy)-2-oxoethyl) 4-spiro[3.3]heptan-2-yl 2-methylenesuccinate (146 mg, 0.41 mmol) in DCM (1 mL). The reaction mixture was stirred for 1 h, diluted with toluene (5 mL) and concentrated. The residue was taken up in EtOAc (10 mL), washed with brine (10 mL), dried (MgSO4) and concentrated to afford the title compound (90 mg, 0.316 mmol) as a colourless oil. 1< H NMR (400 MHz, DMSO-d6) δ 13.08 (br. s, 1H), 6.28 (d, J = 1.2 Hz, 1H), 5.91 (d, J = 1.2 Hz, 1H), 4.82 - 4.73 (m, 1H), 4.64 (s, 2H), 3.34 (s, 2H), 2.43 - 2.34 (m, 2H), 2.02 - 1.90 (m, 6H), 1.85 - 1.74 (m, 2H). LCMS m / z 305.3 (M+Na) +< (ES +< ).Example 20 - 2-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid

[0250]

[0251] Example 20 was prepared using the same procedure as described in Example 2, but using 4-(cyclohexyloxy)-2-methylene-4-oxobutanoic acid (Intermediate 4) instead of 4-octyl itaconate. 1< H NMR (400 MHz, DMSO-d6) δ 13.03 (s, 1H), 6.26 (d, J = 1.3 Hz, 1H), 5.89 (d, J = 1.3 Hz, 1H), 4.96 (q, J = 7.0 Hz, 1H), 4.65 (dq, J = 8.6, 4.0 Hz, 1H), 3.40 - 3.29 (m, 2H), 1.80 - 1.70 (m, 2H), 1.64 (ddd, J = 13.0, 6.9, 3.9 Hz, 2H), 1.42 (d, J = 7.1 Hz, 3H), 1.52 - 1.19 (m, 6H). LCMS m / z 307.6 (M+Na) +< (ES +< ).Example 21 - (R)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid

[0252]

[0253] Example 21 was prepared according to the procedure of Example 12, but using (R)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid (Intermediate 6) instead of (S)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid. 1< H NMR (400 MHz, DMSO-d6) δ 13.09 (s, 1H), 6.28 (d, J = 1.2 Hz, 1H), 5.92 (d, J = 1.2 Hz, 1H), 4.84 - 4.74 (m, 1H), 4.64 (s, 2H), 3.35 (s, 2H), 1.58 - 1.39 (m, 2H), 1.31 - 1.18 (m, 8H), 1.14 (d, J = 6.3 Hz, 3H), 0.91 - 0.82 (m, 3H). LCMS m / z 323.0 (M+Na) +< (ES +< ).

[0254] The sodium salt of Example 21 was made as follows:Example 21a: (R)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid sodium salt

[0255]

[0256] To a solution of (R)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid (1.0 g, 3.3 mmol) in MeCN (10 mL) was added a solution of NaHCO 3 in water (0.5 M, 6.27 mL, 3.14 mmol), and the mixture was stirred at room temperature for 10 min. The mixture was then concentrated under reduced pressure at 30 °C to remove the MeCN, and the remaining aqueous solution was washed with MTBE. The aqueous solution was then concentrated under reduced pressure at 30 °C to remove residual dissolved MTBE, and finally lyophilized to give (R)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid sodium salt (1.0 g, 3.1 mmol, 94 % yield) as a white solid. LCMS (System 2, Method B) m / z 323.2 (M+H) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 6.18 (d, J = 0.8 Hz, 1H), 5.75 (d, J = 0.8 Hz, 1H), 4.82-4.74 (m, 1H), 4.14 (d, J = 1.2 Hz, 2H), 3.25 (s, 2H), 1.52-1.38 (m, 2H), 1.27-1.23 (m, 8H), 1.13 (d, J = 6.4 Hz, 3H), 0.85 (t, J = 6.4 Hz, 3H).Example 22 - 2-((4-((4,4-difluorocyclohexyl)methoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0257]

[0258] Example 22 was prepared according to the procedure of Example 19, but using 4,4-difluorocyclohexanol instead of spiro[3.3]heptan-2-ol. 1< H NMR (400 MHz, DMSO-d6) δ 13.08 (s, 1H), 6.30 (d, J = 1.2 Hz, 1H), 5.94 (d, J = 1.3 Hz, 1H), 4.65 (s, 2H), 3.92 (d, J = 6.1 Hz, 2H), 3.41 (s, 2H), 2.07 - 1.93 (m, 2H), 1.89 - 1.68 (m, 5H), 1.28 - 1.14 (m, 2H). LCMS m / z 319.1 (M-H) -< (ES -< ).Example 23 - 2-((4-(3-ethoxypropoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0259] Step 1

[0260] EDC.HCl (0.371 g, 1.935 mmol) was added to a solution of 3-((2-(tert-butoxy)-2-oxoethoxy)carbonyl)but-3-enoic acid (Intermediate 9, 0.35 g, 1.29 mmol), 3-ethoxypropan-1-ol (0.18 mL, 1.56 mmol), DMAP (0.016 g, 0.13 mmol) and DIPEA (0.34 mL, 1.95 mmol) in DCM (10 mL). The reaction mixture was stirred at RT for 18 h. The reaction mixture was diluted with water (50 mL) and extracted with DCM (3 x 20 mL). The organic layers were combined and passed through a phase separator and concentrated in vacuo. The crude product was purified by chromatography on silica gel (0-10% EtOAc / DCM) to afford impure product. The crude product was purified by chromatography on silica gel (0-20% EtOAc / DCM) to afford 1-(2-(tert-butoxy)-2-oxoethyl) 4-(3-ethoxypropyl) 2-methylenesuccinate (260 mg, 0.71 mmol) as a colourless oil. 1< H NMR (400 MHz, DMSO-d6) δ 6.30 (d, J = 1.2 Hz, 1H), 5.95 - 5.92 (m, 1H), 4.62 (s, 2H), 4.07 (t, J = 6.5 Hz, 2H), 3.43 - 3.35 (m, 6H), 1.82 - 1.71 (m, 2H), 1.42 (s, 9H), 1.10 (t, J = 7.0 Hz, 3H). LCMS m / z 353.3 (M+Na) +< (ES +< ).Step 2

[0261] TFA (2.5 mL) was added to a solution of 1-(2-(tert-butoxy)-2-oxoethyl) 4-(3-ethoxypropyl) 2-methylenesuccinate (260 mg, 0.71 mmol) in DCM (2.5 mL). The reaction mixture was stirred for 2 h, then concentrated. The crude product was purified by chromatography on RP Flash C18 (5-75% MeCN / Water 0.1% Formic Acid) followed by chromatography on silica gel (0-10% MeOH / DCM) to afford the title compound (0.015 g, 0.052 mmol) as a clear and colourless gum. 1< H NMR (400 MHz, DMSO-d6) δ 6.21 (d, J = 1.5 Hz, 1H), 5.82 - 5.77 (m, 1H), 4.20 (s, 2H), 4.06 (t, J = 6.5 Hz, 2H), 3.45 - 3.36 (m, 4H), 3.34 (s, 2H), 1.82 - 1.71 (m, 2H), 1.10 (t, J = 7.0 Hz, 3H). LCMS m / z 275.3 (M+H) +< (ES +< ).Example 24 - 2-((4-(bicyclo[2.2.1]heptan-2-yloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0262]

[0263] Example 24 was prepared according to the procedure of Example 23, but using bicyclo[2.2.1]heptan-2-ol instead of 3-ethoxypropan-1-ol. 1< H NMR (400 MHz, DMSO-d6) δ 13.10 (s, 1H), 6.27 (d, J = 1.2 Hz, 1H), 5.96 - 5.86 (m, 1H), 4.64 (s, 2H), 4.55 - 4.47 (m, 1H), 3.33 (s, 2H), 2.29 - 2.17 (m, 2H), 1.69 - 1.61 (m, 1H), 1.52 - 1.29 (m, 4H), 1.16 - 1.03 (m, 3H). LCMS m / z 305.2 (M+Na) +< (ES +< ).Example 25 - 2-((4-cyclobutoxy-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0264]

[0265] Example 25 was prepared according to the procedure of Example 23, but using cyclobutanol instead of 3-ethoxypropan-1-ol. 1< H NMR (400 MHz, DMSO-d6) δ 13.08 (s, 1H), 6.29 (s, 1H), 5.92 (d, J = 1.5 Hz, 1H), 4.89 (p, J = 7.4 Hz, 1H), 4.65 (s, 2H), 3.36 (s, 2H), 2.30 - 2.19 (m, 2H), 2.06 - 1.92 (m, 2H), 1.79 - 1.68 (m, 1H), 1.65 - 1.50 (m, 1H). LCMS m / z 265.1 (M+Na) +< (ES +< ).Example 26 - 2-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)-3,3,3-trifluoropropanoic acid

[0266] Step 1

[0267] 1-(chloromethyl)-4-methoxybenzene (0.90 mL, 6.64 mmol) was added to a mixture of 3,3,3-trifluoro-2-hydroxypropanoic acid (1.00 g, 6.94 mmol) and cesium carbonate (2.26 g, 6.94 mmol) in dimethylformamide (30 mL). The mixture was stirred at RT for 3 h, then heated to 70 °C for 2 h, then cooled to RT and stirred for 18 h. The mixture was poured onto water (50 mL) and extracted with EtOAc (3 x 50 mL). The combined organic phases were washed with brine (100 mL), dried (MgSO 4 ) and concentrated. The crude product was purified by chromatography on silica gel (0-50% EtOAc / isohexane) to afford 4-methoxybenzyl 3,3,3-trifluoro-2-hydroxypropanoate (640 mg, 2.30 mmol) as a colourless oil. 1< H NMR (400 MHz, DMSO-d6) δ 7.36 - 7.30 (m, 2H), 7.14 (d, J = 7.4 Hz, 1H), 7.00 - 6.91 (m, 2H), 5.22 - 5.14 (m, 2H), 4.92 - 4.83 (m, 1H), 3.76 (s, 3H).Step 2

[0268] EDC.HCl (0.348 g, 1.81 mmol) was added to a solution of 4-(cyclohexyloxy)-2-methylene-4-oxobutanoic acid (Intermediate 4, 0.35 g, 1.65 mmol), 4-methoxybenzyl 3,3,3-trifluoro-2-hydroxypropanoate (0.523 g, 1.98 mmol), DMAP (0.020 g, 0.17 mmol) and DIPEA (0.43 mL, 2.47 mmol) in DCM (8 mL) at 0 °C. The reaction mixture was allowed to warm to RT and stirred for 72 h. The reaction mixture was diluted with 1 M HCl (30 mL). The phases were separated and the aqueous phase was extracted with DCM (10 mL). The combined organic phases were washed with brine (30 mL), dried (MgSO 4 ) and concentrated. The crude product was purified by chromatography on silica gel (0-20% EtOAc / isohexane) to afford 4-cyclohexyl 1-(1,1,1-trifluoro-3-((4-methoxybenzyl)oxy)-3-oxopropan-2-yl) 2-methylenesuccinate (302 mg, 0.593 mmol) as a colourless oil. 1< H NMR (400 MHz, DMSO-d6) δ 7.34 - 7.29 (m, 2H), 6.98 - 6.92 (m, 2H), 6.36 (d, J = 0.9 Hz, 1H), 6.15 (q, J = 7.3 Hz, 1H), 6.06 (d, J = 1.1 Hz, 1H), 5.23 (s, 2H), 4.70 - 4.61 (m, 1H), 3.76 (s, 3H), 3.41 (s, 2H), 1.79 - 1.71 (m, 2H), 1.68 - 1.59 (m, 2H), 1.54 - 1.42 (m, 1H), 1.41 - 1.21 (m, 5H). LCMS m / z 481.3 (M+Na) +< (ES +< ).Step 3

[0269] TFA (1 mL) was added to a solution of 4-cyclohexyl 1-(1,1,1-trifluoro-3-((4-methoxybenzyl)oxy)-3-oxopropan-2-yl) 2-methylenesuccinate (302 mg, 0.659 mmol) in DCM (2 mL). The reaction mixture was stirred for 1 h, diluted with toluene (5 mL) and concentrated. The crude product was purified by chromatography on silica gel (0-60% EtOAc / isohexane) to afford the title compound (38 mg, 0.107 mmol, 16.20 % yield) as a colourless oil. 1< H NMR (400 MHz, DMSO-d6) δ 14.49 (br. s, 1H), 6.35 (d, J = 0.9 Hz, 1H), 6.04 (d, J = 1.1 Hz, 1H), 5.87 (q, J = 7.6 Hz, 1H), 4.70 - 4.60 (m, 1H), 3.47 - 3.35 (m, 2H), 1.80 - 1.71 (m, 2H), 1.69 - 1.58 (m, 2H), 1.52 - 1.43 (m, 1H), 1.42 - 1.18 (m, 5H). LCMS m / z 337.2 (M-H) -< (ES -< ).Example 27 - 2-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)-3,3,3-trifluoropropanoic acid

[0270]

[0271] Example 27 was prepared according to the procedure of Example 26, but using 4-(cyclooctyloxy)-2-methylene-4-oxobutanoic acid (Intermediate 1) instead of 4-(cyclohexyloxy)-2-methylene-4-oxobutanoic acid. 1< H NMR (400 MHz, DMSO-d6) δ 14.47 (s, 1H), 6.34 (s, 1H), 6.03 (s, 1H), 5.86 (q, J = 7.6 Hz, 1H), 4.82 (tt, J = 8.2, 4.0 Hz, 1H), 3.37 (d, J = 2.1 Hz, 2H), 1.80 - 1.32 (m, 12H). LCMS m / z 390.0 (M+H) +< (ES +< ).Example 28 - 3-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)-4,4,4-trifluorobutanoic acid

[0272] Step 1

[0273] 1-(chloromethyl)-4-methoxybenzene (0.86 ml, 6.3 mmol) was added to a mixture of 4,4,4-trifluoro-3-hydroxybutanoic acid (1.00 g, 6.33 mmol) and cesium carbonate (2.06 g, 6.33 mmol) in DMF (27 mL). The mixture was stirred at RT for 1 h, then heated to 70 °C for 2 h. The mixture was cooled to RT and stirred for 18 h. The mixture was poured onto water (50 mL) and extracted with EtOAc (3x50 mL). The combined organic phases were washed with brine (100 mL), dried (MgSO4) and concentrated. The crude product was purified by chromatography on silica gel (0-100% EtOAc / isohexane) to afford 4-methoxybenzyl 4,4,4-trifluoro-3-hydroxybutanoate (0.283 g, 0.97 mmol) as a colourless oil. 1H NMR (400 MHz, DMSO-d6) δ 7.37 - 7.26 (m, 2H), 6.98 - 6.87 (m, 2H), 6.58 (d, J = 6.7 Hz, 1H), 5.08 (d, J = 13.5 Hz, 1H), 5.05 (d, J = 13.6 Hz, 1H), 4.35 (dtt, J = 17.3, 7.2, 3.3 Hz, 1H), 3.75 (s, 3H), 2.77 (dd, J = 15.8, 3.3 Hz, 1H), 2.57 - 2.52 (m, 1H).Step 2

[0274] A slurry of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (0.28 g, 1.5 mmol) in DCM (1.6 mL) was added slowly to a solution of 4-(cyclooctyloxy)-2-methylene-4-oxobutanoic acid (0.233 g, 0.97 mmol), 4-methoxybenzyl 4,4,4-trifluoro-3-hydroxybutanoate (0.283 g, 0.97 mmol), DIPEA (0.25 mL, 1.5 mmol) and DMAP (0.012 g, 0.097 mmol) in DCM (1.6 mL) at 0 °C. The mixture was allowed to warm slowly to RT and stirred for 18 h. The mixture was diluted with 1 M HCl (10 mL) and extracted with EtOAc (3x10 mL). The combined organic phases were washed with brine (15 mL), dried (Na2SO4) and concentrated. The crude product was purified by chromatography on silica gel (0-50% EtOAc / isohexane) to afford 4-cyclooctyl 1-(1,1,1-trifluoro-4-((4-methoxybenzyl)oxy)-4-oxobutan-2-yl) 2-methylenesuccinate (0.304 g, 0.56 mmol) as a colourless oil. LCMS m / z 523.2 (M+Na)+ (ES+). 1H NMR (400 MHz, DMSO-d6) δ 7.34 - 7.22 (m, 2H), 6.96 - 6.85 (m, 2H), 6.23 (d, J = 1.0 Hz, 1H), 5.93 (d, J = 1.1 Hz, 1H), 5.81 (dqd, J = 10.3, 6.7, 3.6 Hz, 1H), 5.04 (s, 2H), 4.80 (tt, J = 8.2, 4.0 Hz, 1H), 3.75 (s, 3H), 3.31 (s, 2H), 3.10 (dd, J = 16.8, 3.6 Hz, 1H), 2.87 (dd, J = 16.8, 9.5 Hz, 1H), 1.78 - 1.33 (m, 14H).Step 3

[0275] Trifluoroacetic acid (1.4 mL) was added to a solution of 4-cyclooctyl 1-(1,1,1-trifluoro-4-((4-methoxybenzyl)oxy)-4-oxobutan-2-yl) 2-methylenesuccinate (0.304 g, 0.56 mmol) in DCM (5 mL) at 0 °C. The reaction mixture was warmed to RT, stirred for 30 min and concentrated. The resiude was co-evaporated with toluene (2x10 mL). The crude product was purified by chromatography on silica gel (0-50% EtOAc / DCM) to afford 3-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)-4,4,4-trifluorobutanoic acid (0.127 g, 0.32 mmol) as a colourless oil. LCMS m / z 403.3 (M+Na)+ (ES+). 1H NMR (400 MHz, DMSO-d6) δ 12.84 (s, 1H), 6.29 (d, J = 1.1 Hz, 1H), 5.96 (d, J = 1.2 Hz, 1H), 5.83 - 5.71 (m, 1H), 4.82 (tt, J = 8.1, 3.9 Hz, 1H), 3.35 (s, 2H), 2.96 (dd, J = 16.9, 3.8 Hz, 1H), 2.74 (dd, J = 16.9, 9.2 Hz, 1H), 1.79 - 1.34 (m, 14H).Example 29 - 2-(4-(cycloheptyloxy)-2-methylene-4-oxobutanoyloxy)acetic acid

[0276] Step 1

[0277] To a solution of 3-((2-tert-butoxy-2-oxoethoxy)carbonyl)but-3-enoic acid (234 mg, 0.96 mmol), cycloheptanol (110 mg, 0.96 mmol) and DMAP (117 mg, 0.96 mmol) in DCM (4 mL) was added EDC.HCl (276 mg, 1.44 mmol) at 0 °C, and the resulting mixture was stirred at room temperature for 1 h. The mixture was quenched with aqueous NH 4 Cl (2 mL), the phases were separated, and the aqueous phase was extracted with DCM (2 x 3 mL). The combined organic phases were washed with brine, dried over Na 2 SO 4 and filtered. The filtrate was concentrated under reduced pressure at 40 °C , and the residue was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give 1-(2-tert-butoxy-2-oxoethyl) 4-cycloheptyl 2-methylenesuccinate (130 mg, 0.38 mmol, 40 %) as a pale-yellow oil. LCMS (System 2, Method B) m / z 363.3 (M+Na) +< (ES +< ).Step 2

[0278] A mixture of 1-(2-tert-butoxy-2-oxoethyl) 4-cycloheptyl 2-methylenesuccinate (130 mg, 0.38 mmol) and HCl solution in 1,4-dioxane (4 M, 3 mL, 12 mmol) in DCM (2 mL) was stirred at room temperature for 4 h. The mixture was concentrated under reduced pressure at 40 °C and the residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10 µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.05% TFA / water); gradient: 15-95% MeCN; collection wavelength: 214 nm). The collected fractions were concentrated at 40 °C under reduced pressure to remove MeCN, and the residue was lyophilized to give 2-(4-(cycloheptyloxy)-2-methylene-4-oxobutanoyloxy)acetic acid (76 mg, 0.27 mmol, 70 %) as a pale-yellow oil. LCMS (System 2, Method B) m / z 307.1 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 13.06 (br, 1H), 6.27 (d, J = 0.8 Hz, 1H), 5.91 (d, J = 0.8 Hz, 1H), 4.83-4.78 (m, 1H), 4.63 (s, 2H), 3.33 (s, 2H), 1.83-1.76 (m, 2H), 1.62-1.51 (m, 4H), 1.50-1.48 (m, 4H), 1.42-1.37 (m, 2H).

[0279] The following compounds were made using a similar procedure: Example No. Alcohol Intermediate / Example Structure / Name LCMS / 1< H NMR data 30 octan-3-olLCMS (System 2, Method B) m / z 323.2 (M+Na) +< (ES +< ). 2-(2-methylene-4-(octan-3-yloxy)-4-oxobutanoyloxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.28 (d, J = 0.8 Hz, 1H), 5.93 (d, J = 0.8 Hz, 1H), 4.73-4.66 (m, 1H), 4.62 (s, 2H), 3.37 (s, 2H), 1.56-1.40 (m, 4H), 1.25-1.14 (m, 6H), 0.84 (t, J = 6.4 Hz, 3H), 0.80 (d, J = 7.2 Hz, 3H).31 octan-4-olLCMS (System 2, Method B) m / z 323.2 (M+Na) +< (ES +< ). 2-(2-methylene-4-(octan-4-yloxy)-4-oxobutanoyloxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.28 (d, J = 1.2 Hz, 1H), 5.92 (d, J = 1.2 Hz, 1H), 4.81-4.74 (m, 1H), 4.62 (s, 2H), 3.36 (s, 2H), 1.49-1.42 (m, 4H), 1.32-1.14 (m, 6H), 0.84 (t, J = 7.2 Hz, 6H).32 heptan-4-olLCMS (System 2, Method B) m / z 309.3 (M+Na) +< (ES +< ). 2-((4-(heptan-4-yloxy)-2-methylene-4-oxobutanoyl)oxy) acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.28 (d, J = 1.2 Hz, 1H), 5.93 (d, J = 0.8 Hz, 1H), 4.82-4.77 (m, 1H), 4.62 (s, 2H), 3.37 (s, 2H), 1.48-1.42 (m, 4H), 1.32-1.16 (m, 4H), 0.85 (t, J = 7.2 Hz, 6H).33 adamantan-2-olLCMS (System 2, Method B) m / z 323.2 (M+H) +< (ES +< ). 2-((4-((adamantan-2-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.01 (br, 1H), 6.29 (s, 1H), 5.94 (s, 1H), 4.80 (m, 1H), 4.63 (s, 2H), 3.41 (s, 2H), 1.89-1.86 (m, 4H), 1.80-1.78 (m, 4H), 1.72-1.68 (m, 4H), 1.49 (d, J = 12.4 Hz, 2H).34 1-cyclohexylethan-1-olLCMS (System 2, Method B) m / z 321.3 (M+Na) +< (ES +< ). 2-((4-(1-cyclohexylethoxy)-2-methylene-4-oxobutanoyl)oxy) acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.11 (br, 1H), 6.28 (s, 1H), 5.92 (s, 1H), 4.64-4.58 (m, 1H), 4.62 (s, 2H), 3.40 (s, 2H), 1.68 (d, J = 10.0 Hz, 3H), 1.59 (t, J = 10.8 Hz, 2H), 1.42-1.35 (m, 1H), 1.21-1.12 (m, 3H), 1.09 (d, J = 6.0 Hz, 3H), 1.06-0.84 (m, 2H).35 1-cycloheptylethan-1-olLCMS (System 2, Method B) m / z 335.2 (M+Na) +< (ES +< ). 2-((4-(1-cycloheptylethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.08 (br, 1H), 6.27 (s, 1H), 5.92 (s, 1H), 4.70-4.65 (m, 1H), 4.63 (s, 2H), 3.34 (s, 2H), 1.63-1.61 (m, 5H), 1.57-1.33 (m, 6H), 1.22-1.14 (m, 2H), 1.07 (d, J = 6.0 Hz, 3H).36 spiro[3.4]octan-2-olLCMS (System 2, Method B) m / z 319.1 (M+Na) +< (ES +< ). 2-(2-methylene-4-oxo-4-(spiro[3.4]octan-2-yloxy)butanoyloxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.08 (br, 1H), 6.28 (d, J = 0.8 Hz, 1H), 5.91 (d, J = 0.8 Hz, 1H), 4.90-4.83 (m, 1H), 4.64 (s, 2H), 3.35 (s, 2H), 2.23-2.18 (m, 2H), 1.92-1.87 (m, 2H), 1.57-1.47 (m, 8H).37 spiro[3.5]nonan-2-olLCMS (System 2, Method B) m / z 333.3 (M+Na) +< (ES +< ). 2-(2-methylene-4-oxo-4-(spiro[3.5]nonan-2-yloxy)butanoyloxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.08 (br, 1H), 6.28 (s, 1H), 5.91 (s, 1H), 4.91-4.83 (m, 1H), 4.64 (s, 2H), 3.35 (s, 2H), 2.19-2.14 (m, 2H), 1.68-1.63 (m, 2H), 1.40-1.30 (m, 10H).38 spiro[3.5]nonan-7-olLCMS (System 2, Method B) m / z 333.3 (M+Na) +< (ES +< ). 2-(2-methylene-4-oxo-4-(spiro[3.5]nonan-7-yloxy)butanoyloxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.10 (br, 1H), 6.26 (s, 1H), 5.90 (s, 1H), 4.62 (s, 3H), 3.34 (s, 2H), 1.83-1.77 (m, 2H), 1.70-1.61 (m, 8H), 1.37-1.34 (m, 4H).39 2,2-dimethylcyclohexan-1-olLCMS (System 2, Method B) m / z 321.3 (M+Na) +< (ES +< ). 2-((4-((2,2-dimethylcyclohexyl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.28 (s, 1H), 5.93 (s, 1H), 4.62 (s, 2H), 4.47-4.44 (m, 1H), 3.38 (s, 2H), 1.62-1.58 (m, 2H), 1.42-1.34 (m, 4H), 1.29-1.18 (m, 2H), 0.84 (s, 6H).41 2,2,4,4-tetramethylcyclobutan-1-olLCMS (System 2, Method B) m / z 321.2 (M+Na) +< (ES +< ). 2-((2-methylene-4-oxo-4-(2,2,4,4-tetramethylcyclobutoxy) butanoyl)oxy) acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.11 (br, 1H), 6.29 (s, 1H), 5.95 (s, 1H), 4.63 (s, 2H), 4.35 (s, 1H), 3.43 (s, 2H), 1.51 (d, J = 11.6 Hz, 1H), 1.41 (d, J = 11.6 Hz, 1H), 1.10 (s, 6H), 1.00 (s, 6H).42 (S)-octan-3-olLCMS (System 2, Method B) m / z 323.2 (M+Na) +< (ES +< ). (S)-2-(2-methylene-4-(octan-3-yloxy)-4-oxobutanoyloxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.28 (d, J = 0.8 Hz, 1H), 5.93 (d, J = 0.8 Hz, 1H), 4.73-4.66 (m, 1H), 4.62 (s, 2H), 3.37 (s, 2H), 1.56-1.40 (m, 4H), 1.25-1.14 (m, 6H), 0.84 (t, J = 6.4 Hz, 3H), 0.80 (d, J = 7.2 Hz, 3H).45 (R)-heptan-2-olLCMS (System 2, Method B) m / z 309.3 (M+Na) +< (ES +< ). (R)-2-((4-(heptan-2-yloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.14 (br, 1H), 6.27 (d, J = 0.8 Hz, 1H), 5.91 (d, J = 0.8 Hz, 1H), 4.80-4.75 (m, 1H), 4.63 (s, 2H), 3.34 (s, 2H), 1.49-1.43 (m, 2H), 1.29-1.23 (m, 6H), 1.13 (d, J = 6.4 Hz, 3H), 0.85 (t, J = 6.4 Hz, 3H).46 nonan-2-olLCMS (System 2, Method B) m / z 337.3 (M+Na) +< (ES +< ).2-((2-methylene-4-(nonan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.27 (d, J = 1.2 Hz, 1H), 5.91 (d, J = 1.2 Hz, 1H), 4.82-4.74 (m, 1H), 4.62 (s, 2H), 3.50 (s, 2H), 1.48-1.43 (m, 2H), 1.29-1.23 (m, 10H), 1.13 (d, J = 6.4 Hz, 3H), 0.86 (t, J = 6.8 Hz, 3H).47 nonan-5-olLCMS (System 2, Method B) m / z 337.3 (M+Na) +< (ES +< ). 2-((2-methylene-4-(nonan-5-yloxy)-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.14 (br, 1H), 6.28 (d, J = 1.2 Hz, 1H), 5.92 (d, J = 0.8 Hz, 1H), 4.79-4.74 (m, 1H), 4.62 (s, 2H), 3.37 (s, 2H), 1.52-1.42 (m, 4H), 1.31-1.14 (m, 8H), 0.85 (t, J = 6.8 Hz, 6H).48 1-(3,5-dichlorophenyl)ethan-1-olLCMS (System 2, Method B) m / z 383.0 (M+Na) +< (ES +< ). 2-((4-(1-(3,5-dichlorophenyl)ethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.12 (br, 1H), 7.54 (t, J = 2.0 Hz, 1H), 7.41 (d, J = 1.6 Hz, 2H), 6.31 (d, J = 0.8 Hz, 1H), 5.96 (d, J = 0.8 Hz, 1H), 5.77 (q, J = 6.4 Hz, 1H), 4.63 (s, 2H), 3.47 (s, 2H), 1.44 (d, J = 6.4 Hz, 3H).49 1-(3,5-dichlorophenyl)propan-2-olLCMS (System 2, Method B) m / z 397.1 (M+Na) +< (ES +< ). 2-((4-((1-(3,5-dichlorophenyl)propan-2-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.07 (br, 1H), 7.46 (t, J = 2.0 Hz, 1H ), 7.29 (d, J = 1.6 Hz, 2H), 6.27 (s, 1H), 5.87 (d, J = 0.8 Hz,1H ), 4.99 (q, J = 6.4 Hz, 1H), 4.61 (d, J =1.6 Hz, 2H), 3.31 (s, 2H), 2.88-2.78 (m, 2H), 1.14 (d, J = 6.4 Hz, 3H).50 (R)-octan-3-olLCMS (System 2, Method B) m / z 323.2 (M+Na) +< (ES +< ). (R)-2-(2-methylene-4-(octan-3-yloxy)-4-oxobutanoyloxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.11 (br, 1H), 6.28 (d, J = 0.8 Hz, 1H), 5.93 (d, J = 0.8 Hz, 1H), 4.73-4.66 (m, 1H), 4.62 (s, 2H), 3.37 (s, 2H), 1.56-1.40 (m, 4H), 1.25-1.14 (m, 6H), 0.84 (t, J = 6.4 Hz, 3H), 0.80 (d, J = 7.2 Hz, 3H).51 (1R,2S,4R)-1,7,7-trimethylbicyclo[2.2.1] heptan-2-ol (CAS No. 464-43-7)LCMS (System 2, Method B) m / z 347.2 (M+Na) +< (ES +< ). 2-((2-methylene-4-oxo-4-(((1R,2S,4R)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)butanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.13 (br, 1H), 6.29 (s, 1H), 5.94 (d, J = 0.8 Hz, 1H), 4.79-4.77 (m, 1H), 4.64 (s, 2H), 3.40 (s, 2H), 2.27-2.20 (m, 1H), 1.84-1.77 (m, 1H), 1.72-1.63 (m, 2H), 1.27-1.12 (m, 2H), 0.91 (dd, J = 13.6, 3.6 Hz, 1H), 0.84 (d, J = 8.8 Hz, 6H), 0.76 (s, 3H).52 1-cyclohexyl-2,2,2-trifluoroethan-1-olLCMS (System 2, Method B) m / z 375.3 (M+Na) +< (ES +< ). 2-((4-(1-cyclohexyl-2,2,2-trifluoroethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.08 (br, 1H), 6.33 (s, 1H), 6.01 (s, 1H), 5.27-5.18 (m, 1H), 4.63 (s, 2H), 3.56 (s, 2H), 1.88-1.75 (m, 1H), 1.71-1.51 (m, 5H), 1.29-0.96 (m, 5H).53 bicyclo[3.3.1]nonan-9-olLCMS (System 2, Method B) m / z 333.2 (M+Na) +< (ES +< ). 2-((4-(bicyclo[3.3.1]nonan-9-yloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.08 (br, 1H), 6.29 (d, J = 1.2 Hz, 1H), 5.94 (d, J = 1.2 Hz, 1H), 4.66 (s, 1H), 4.63 (s, 2H), 3.41 (s, 2H), 1.83-1.75 (m, 6H), 1.73 (d, J = 13.2 Hz, 4H), 1.45 (d, J = 6.0 Hz, 4H).54 (S)-1,1,1-trifluorooctan-2-olLCMS (System 2, Method B) m / z 377.1 (M+Na) +< (ES +< ). (S)-2-((2-methylene-4-oxo-4-((1,1,1-trifluorooctan-2-yl)oxy)butanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.10 (br, 1H), 6.34 (s,1H), 6.02 (s, 1H), 5.41-5.36 (m, 1H), 4.62 (s, 2H), 3.54 (s, 2H), 1.75-1.62 (m, 2H), 1.26-1.24 (m, 8H), 0.86 (t, J = 6.4 Hz, 3H).55 (R)-nonan-2-olLCMS (System 2, Method B) m / z 337.3 (M+Na) +< (ES +< ). (R)-2-((2-methylene-4-(nonan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.06 (br, 1H), 6.27 (d, J = 1.2 Hz, 1H), 5.91 (d, J = 1.2 Hz, 1H), 4.82-4.74 (m, 1H), 4.62 (s, 2H), 3.50 (s, 2H), 1.48-1.43 (m, 2H), 1.29-1.23 (m, 10H), 1.13 (d, J = 6.4 Hz, 3H), 0.86 (t, J = 6.8 Hz, 3H).56 (R)-1-cyclohexylethan-1-olLCMS (System 2, Method B) m / z 321.3 (M+Na) +< (ES +< ). (R)-2-((4-(1-cyclohexylethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.14 (br, 1H), 6.28 (s,1H), 5.92 (s, 1H), 4.62 (s, 2H), 4.66-4.58 (m, 1H), 3.26 (s, 2H), 1.69-1.67 (m, 3H), 1.62-1.53 (m, 2H), 1.42-1.35 (m, 1H), 1.25-1.08 (m, 3H), 1.15 (d, J = 6.4 Hz, 3H), 1.04-0.82 (m, 2H). Example 40 - 2-((4-(cyclooctyloxy)-3-methyl-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0280] Step 1

[0281] To a solution of 4-(cyclooctyloxy)-2-methylene-4-oxobutanoic acid (570 mg, 2.38 mmol) in THF (10 mL) at -78 °C was added a solution of LDA in THF / n-heptane / ethyl benzene (2 M, 2.38 mL, 4.76 mmol), and the reaction mixture was stirred at -78 °C for 1 h. lodomethane (338 mg, 2.38 mmol) was then added at -78 °C, and the reaction mixture was stirred at -78 °C for 2 h. The reaction mixture was quenched with dilute aqueous HCl (0.5 M, 20 mL) and extracted with EtOAc (2 x 20 mL). The combined organic phases were dried over Na 2 SO 4 , filtered and the filtrate was concentrated under reduced pressure at 35 °C to give crude 4-(cyclooctyloxy)-3-methyl-2-methylene-4-oxobutanoic acid (550 mg, 2.16 mmol, 91%) as a colorless oil, which was used in the next step without purification. LCMS (System 2, Method C) m / z 277.4 (M+Na) +< (ES +< ).Step 2

[0282] To a solution of 4-(cyclooctyloxy)-3-methyl-2-methylene-4-oxobutanoic acid (550 mg, 2.16 mmol) in acetone (10 mL) was added potassium carbonate (329 mg, 2.39 mmol), and the reaction mixture was stirred at room temperature for 30 min. tert-Butyl 2-bromoacetate (464 mg, 2.39 mmol) was then added and the resulting mixture was stirred at room temperature overnight. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give 1-(2-(tert-butoxy)-2-oxoethyl) 4-cyclooctyl 3-methyl-2-methylenesuccinate (380 mg, 1.0 mmol, 48%) as a yellow oil. LCMS (System 2, Method B) m / z 391.3 (M+Na) +< (ES +< ).Step 3

[0283] A mixture of 1-(2-(tert-butoxy)-2-oxoethyl) 4-cyclooctyl 3-methyl-2-methylenesuccinate (180 mg, 0.49 mmol) and HCl solution in 1,4-dioxane (4 M, 2 mL, 8.0 mmol) in DCM (2 mL) was stirred at room temperature for 4 h, then concentrated under reduced pressure at 40 °C. The residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.05% TFA / water) gradient: 30-95% MeCN; collection wavelength: 214 nm). The fractions were concentrated under reduced pressure at 40 °C to remove MeCN, and the residue was lyophilized to give 2-((4-(cyclooctyloxy)-3-methyl-2-methylene-4-oxobutanoyl)oxy)acetic acid (117 mg, 0.37 mmol, 77 %) as a pale-yellow oil.

[0284] LCMS (System 2, Method B) m / z 335.2 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 13.10 (br, 1H), 6.28 (s, 1H), 5.85 (s, 1H), 4.82-4.76 (m, 1H), 4.64 (d, J = 1.6 Hz, 2H), 3.51 (q, J = 6.8 Hz, 1H), 1.70-1.62 (m, 6H), 1.55-1.41 (m, 8H), 1.27 (d, J = 7.2 Hz, 3H).Example 43 - 2-((4-(cyclooctyloxy)-3-methoxy-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0285] Step 1

[0286] A mixture of (2R,3R)-2,3-dihydroxysuccinic acid (3 g, 20 mmol), cyclooctanol (7.7 g, 60 mmol), anhydrous p-toluenesulfonic acid (344 mg, 2 mmol) and anhydrous Na 2 SO 4 (6 g, 42.2 mmol) in toluene (40 mL) was stirred at 80 °C for 16 h. The reaction mixture was cooled to room temperature and filtered. The filtrate was concentrated under reduced pressure at 45 °C and the residue was purified by reversed phase column chromatography (120 g C18 silica; flow rate: 40 mL / min; 50-70% MeCN / (10 mM HCl / water); collection wavelength: 214 nm). The collected fractions were concentrated under reduced pressure at 40 °C to remove MeCN, and the residue was lyophilized to give dicyclooctyl (2R,3R)-2,3-dihydroxysuccinate (3.5 g, 9.4 mmol, 47 %) as a white solid. LCMS (System 2, Method B) m / z 393.3 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 5.38 (d, J = 8.0 Hz, 2H), 4.90-4.86 (m, 2H), 4.32 (d, J = 7.6 Hz, 2H), 1.77-1.65 (m, 12H), 1.58-1.49 (m, 16H).Step 2

[0287] To a solution of dicyclooctyl (2R,3R)-2,3-dihydroxysuccinate (3.5 g, 9.4 mmol) in THF:H 2 O (2:1, 40 mL) was added NalO 4 (2.3 g, 18.8 mmol) at room temperature, and the reaction mixture was stirred at room temperature for 2 h. The reaction mixture was filtered, the filtrate was diluted with EtOAc (30 mL), quenched with saturated aqueous Na 2 S 2 O 3 aq. and separated. The organic layer was washed with saturated aqueous NaHCO 3 , dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 30 °C. The residue was purified by flash column chromatography (40 g silica, 0-20% EtOAc / petroleum ether) to give crude cyclooctyl 2-oxoacetate (3 g, 16.3 mmol, 87 %) as a pale yellow oil, which was used directly in the next step. 1< H NMR (400 MHz, CDCl 3 ) δ: 9.39 (s, 1H), 5.08-5.03 (m, 1H), 1.91-1.68 (m, 6H), 1.60-1.58 (m, 8H).Step 3

[0288] A mixture of cyclooctyl 2-oxoacetate (3 g, 16.3 mmol), tert-butyl acrylate (3.1 g, 24.4 mmol) and DABCO (914 mg, 8.1 mmol) in a solvent mixture of 1,4-dioxane / DMSO / H 2 O (20 mL, 8 / 2 / 1) was stirred at room temperature for 48 h. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure at 50 °C. The residue was purified by reversed phase column chromatography (120 g C18 silica; flow rate: 40 mL / min; 50-70% MeCN / H 2 O; collection wavelength: 214 nm). The collected fractions were concentrated under reduced pressure at 40 °C to remove MeCN, and the residue was lyophilized to give 1-(tert-butyl) 4-cyclooctyl 3-hydroxy-2-methylenesuccinate (2.3 g, 7.4 mmol, 45 %) as a yellow oil. LCMS (System 2, Method B) m / z 335.2 (M+Na) +< (ES +< ).Step 4

[0289] To a mixture of 1-(tert-butyl) 4-cyclooctyl 3-hydroxy-2-methylenesuccinate (300 mg, 0.96 mmol) and Ag 2 O (445 mg, 1.92 mmol) in DCM (1 mL) was added methyl iodide (818 mg, 5.76 mmol), and the reaction mixture was stirred at 40 °C for 16 h. The mixture was filtered and the filtrate was concentrated under reduced pressure at 30 °C to give the crude product as a 5:1 mixture of 1-(tert-butyl) 4-cyclooctyl 3-methoxy-2-methylenesuccinate and 1-(tert-butyl) 4-cyclooctyl 3-methoxy-2-methylfumarate respectively. The crude product was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give pure 1-(tert-butyl) 4-cyclooctyl 3-methoxy-2-methylenesuccinate (250 mg, 0.77 mmol, 79%) as a pale yellow oil. LCMS (System 2, Method B) m / z 349.3 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, CDCl 3 ) δ: 6.31 (d, J = 0.8 Hz, 1H), 5.88 (s, 1H), 5.02-4.98 (m, 1H), 4.58 (s, 1H), 3.44 (s, 3H), 1.86-1.64 (m, 6H), 1.63-1.52 (m, 8H), 1.48 (s, 9H).Step 5

[0290] A mixture of 1-(tert-butyl) 4-cyclooctyl 3-methoxy-2-methylenesuccinate (230 mg, 0.71 mmol) and TFA (2 mL) in DCM (2 mL) was stirred at room temperature overnight. The mixture was concentrated under reduced pressure at 40 °C to give crude 4-(cyclooctyloxy)-3-methoxy-2-methylene-4-oxobutanoic acid (200 mg, 0.71 mmol, > 100 %) as a pale yellow oil, which was used directly in the next step. LCMS (System 2, Method C) m / z 293.4 (M+Na) +< (ES +< ).Step 6

[0291] To a solution of crude 4-(cyclooctyloxy)-3-methoxy-2-methylene-4-oxobutanoic acid (200 mg, 0.71 mmol) in acetone (5 mL) was added potassium carbonate (110 mg, 0.74 mmol), and the reaction mixture was stirred at room temperature for 30 min. Tert-butyl 2-bromoacetate (171 mg, 0.89 mmol) was added and the resulting mixture was stirred at room temperature overnight. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give 1-(2-(tert-butoxy)-2-oxoethyl) 4-cyclooctyl 3-methoxy-2-methylenesuccinate (200 mg, 0.52 mmol, 73%) as a yellow oil. LCMS (System 2, Method B) m / z 407.3 (M+Na) +< (ES +< ).Step 7

[0292] A mixture of 1-(2-(tert-butoxy)-2-oxoethyl) 4-cyclooctyl 3-methoxy-2-methylenesuccinate (200 mg, 0.52 mmol) and TFA (2 mL) in DCM (2 mL) was stirred at room temperature for 3 h. The mixture was concentrated under reduced pressure at 40 °C and the residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.1% TFA / water) gradient: 45-95% MeCN; collection wavelength: 214 nm). The fractions were concentrated under reduced pressure at 40 °C to remove MeCN, and the residue was lyophilized to give 2-((4-(cyclooctyloxy)-3-methoxy-2-methylene-4-oxobutanoyl)oxy)acetic acid (144 mg, 0.44 mmol, 84%) as a white solid. LCMS (System 2, Method B) m / z 351.2 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 12.97 (br, 1H), 6.40 (s, 1H), 6.03 (s, 1H), 4.90-4.84 (m, 1H), 4.65 (s, 2H), 4.60 (s, 1H), 3.33 (s, 3H), 1.77-1.54 (m, 6H), 1.49-1.38 (m, 8H).Example 44 - 2-((4-((-adamantan-1-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0293] Step 1

[0294] A mixture of adamantan-1-ol (3.1 g, 20 mmol), bromoacetic acid (5.5 g, 40 mmol) and anhydrous p-toluenesulfonic acid (172 mg, 1 mmol) in toluene (100 mL) was heated at reflux overnight. The reaction mixture was cooled to room temperature, diluted with saturated aqueous NaHCO 3 , separated and the aqueous layer was extracted with EtOAc (2 x 30 mL). The combined organic layers was washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (80 g silica, 0-5% EtOAc / petroleum ether) to give adamantan-1-yl 2-bromoacetate (4.5 g, 16.5 mmol, 83%) as a white solid. 1< H NMR (400 MHz, DMSO-d6) δ: 4.02 (s, 2H), 2.50 (m, 3H), 2.05 (d, J = 3.2 Hz, 6H), 1.62 (t, J = 2.8 Hz, 6H).Step 2

[0295] To a solution of ethyl 2-(diethoxyphosphoryl)acetate (1.2 g, 5.4 mmol) in THF (10 mL) was added NaH suspension in mineral oil (60 wt. %, 236 mg, 5.9 mmol) at 0 °C, the reaction mixture was stirred at 0 °C for 0.5 h, then adamantan-1-yl 2-bromoacetate (1.61 g, 5.9 mmol) was added. The reaction mixture was stirred at room temperature for 4 h, then it was quenched by the addition of dilute aqueous HCl (0.5 M, 10 mL), adjusted to pH = 5, and extracted with EtOAc (2 x 10 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (40 g silica, 0-30% EtOAc / petroleum ether) to give crude 4-(adamantan-1-yl) 1-ethyl 2-(diethoxyphosphoryl)succinate (2 g, 4.8 mmol, 90%) as a yellow oil. LCMS (System 2, Method B) m / z 417.3 (M+H) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 4.13-4.00 (m, 6H), 3.37-3.32 (m, 1H), 2.75-2.72 (m, 1H), 2.63-2.60 (m, 1H), 2.11 (m, 3H), 2.01(s, 6H), 1.60 (m, 6H), 1.26-1.17 (m, 9H).Step 3

[0296] To a solution of 4-(adamantan-1-yl) 1-ethyl 2-(diethoxyphosphoryl)succinate (2 g, 4.8 mmol) and potassium carbonate (1.3 g, 9.6 mmol) in DMF (20 mL) was added formaldehyde solution in water (37 wt. %, 7.8 mL, 96 mmol) at room temperature, and the reaction mixture was stirred at room temperature for 4 h. The reaction mixture was diluted with H 2 O (60 mL) and extracted with MTBE (2 x 30 mL). The combined organic layers were washed with H 2 O and brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 30 °C. The residue was purified by flash column chromatography (40 g silica, 0-10% MTBE / petroleum ether) to give 4-(adamantan-1-yl) 1-ethyl 2-methylenesuccinate (800 mg, 3.0 mmol, 57%) as a yellow oil. LCMS (System 2, Method B) m / z 315.3 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 6.14 (d, J = 1.2 Hz, 1H), 5.75 (d, J = 1.2 Hz, 1H), 4.13 (q, J = 7.2 Hz, 2H), 3.26 (s, 2H), 2.11 (m, 3H), 2.01 (d, J = 2.8 Hz, 6H), 1.60 (s, 6H), 1.21 (t, J = 7.8 Hz, 3H).Step 4

[0297] To a solution of 4-(adamantan-1-yl) 1-ethyl 2-methylenesuccinate (800 mg, 3.0 mmol) in THF (10 mL) was added LiOH solution in water (2 M, 4.5 mL, 9 mmol), and the reaction mixture was stirred at room temperature overnight. The reaction mixture was concentrated under reduced pressure at 30 °C to remove THF. The residue was diluted with H 2 O (20 mL) and washed with MTBE (2 x 10 mL). The aqueous layer was adjusted to pH = 3 using dilute aqueous HCl (0.5 M) and extracted with EtOAc (2 x 10 mL). The combined EtOAc layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C to give a 1:1 mixture of 4-((adamantan-1-yl)oxy)-2-methylene-4-oxobutanoic acid and 4-((adamantan-1-yl)oxy)-2-methyl-4-oxobut-2-enoic acid (300 mg, 1.1 mmol, 38%) as a pale yellow oil, which was used directly in the next step. LCMS (System 2, Method C) m / z 287.2 (M+Na) +< (ES +< ).Step 5

[0298] To a solution of a 1:1 mixture of 4-((adamantan-1-yl)oxy)-2-methylene-4-oxobutanoic acid and 4-((adamantan-1-yl)oxy)-2-methyl-4-oxobut-2-enoic acid (300 mg, 1.1 mmol), and potassium carbonate (157 mg, 1.1 mmol) in acetone (10 mL) was added 2,2,2-trichloroethyl 2-bromoacetate (354 mg, 1.3 mmol) at room temperature, and the reaction mixture was stirred at room temperature overnight. The reaction mixture was then filtered, and the filtrate was concentrated under reduced pressure at 30 °C. The residue was purified by flash column chromatography (40 g silica, 0-10% MTBE / petroleum ether) to give 4-(adamantan-1-yl) 1-(2-oxo-2-(2,2,2-trichloroethoxy)ethyl) 2-methylenesuccinate (270 mg, 0.60 mmol, 54%) as a yellow oil. LCMS (System 2, Method B) m / z 475.3 (M+Na) +< (ES +< ).Step 6

[0299] To a solution of 4-(adamantan-1-yl) 1-(2-oxo-2-(2,2,2-trichloroethoxy)ethyl) 2-methylenesuccinate (270 mg, 0.60 mmol) in AcOH (5 mL) was added zinc powder (195 mg, 3 mmol), and the reaction mixture was stirred at room temperature overnight. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure at 45 °C. The residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.05% TFA / water) gradient: 50-95% MeCN; collection wavelength: 214 nm). The fractions were concentrated under reduced pressure at 40 °C to remove MeCN, and the residue was lyophilized to give 2-((4-((-adamantan-1-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid (72 mg, 37 %) as a colorless oil. LCMS (System 2, Method B) m / z 345.3 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 13.07 (br, 1H), 6.23 (s, 1H), 5.86 (s, 1H), 4.64 (s, 2H), 3.27 (s, 2H), 2.10 (s, 3H), 2.01 (d, J = 2.4 Hz, 6H), 1.60 (m, 6H).Example 57 - (R)-4,4,4-trifluoro-3-((2-methylene-4-(((R)-octan-2-yl)oxy)-4-oxobutanoyl)oxy)butanoic acid

[0300] Step 1

[0301] To a mixture of (R)-4,4,4-trifluoro-3-hydroxybutanoic acid (300 mg, 1.90 mmol) and K 2 CO 3 (315 mg, 2.28 mmol) in DMF (10 mL) was added PMBCl (327 mg, 2.09 mmol) at 0 °C, and the reaction mixture was stirred at room temperature overnight. The reaction mixture was quenched with water and extracted with EtOAc. The organic layers were washed with water and brine, dried over Na2SO4 and filtered. The filtrate was concentrated under reduced pressure at 40 °C, and the residue was purified by flash column chromatography (80 g silica, 0-20% MTBE / petroleum ether) to give 4-methoxybenzyl (R)-4,4,4-trifluoro-3-hydroxybutanoate (320 mg, 1.15 mmol, 61 %) as a yellow oil. LCMS (System 2, Method B) m / z 301.1 (M+Na) +< (ES +< ).Step 2

[0302] To a solution of (R)-2-methylene-4-(octan-2-yloxy)-4-oxobutanoic acid (228 mg, 1.15 mmol), 4-methoxybenzyl (R)-4,4,4-trifluoro-3-hydroxybutanoate (320 mg, 1.15 mmol) and DMAP (140 mg, 1.15 mmol) in DCM (3 mL) was added EDC.HCl (331 mg, 1.725 mmol) at 0 °C, and the resulting pale yellow mixture was stirred at room temperature for 2 h. The mixture was quenched with saturated aqueous NH 4 Cl solution (1 mL), separated and the organic phase was extracted with DCM (2 x 2 mL). The separated organic phases were washed with brine, dried over Na2SO4 and filtered. The filtrate was concentrated under reduced pressure at 40 °C, and the residue was purified by flash column chromatography (25 g silica, 0-15% MTBE / petroleum ether) to give 4-((R)-octan-2-yl) 1-((R)-1,1,1-trifluoro-4-((4-methoxybenzyl)oxy)-4-oxobutan-2-yl) 2-methylenesuccinate (300 mg, 0.60 mmol, 52 %) as a yellow oil. LCMS (System 2, Method C) m / z 525.3 (M+Na) +< (ES +< ).Step 3

[0303] A solution of 4-((R)-octan-2-yl) 1-((R)-1,1,1-trifluoro-4-((4-methoxybenzyl)oxy)-4-oxobutan-2-yl) 2-methylenesuccinate (300 mg, 0.60 mmol) in HCl solution in 1,4-dioxane (4 M, 3 mL) was stirred at room temperature for 4 h. The mixture was concentrated under reduced pressure at 30 °C and the residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.05% TFA / water) gradient: 60-95% MeCN; collection wavelength: 214 nm). The fractions were concentrated under reduced pressure at 40 °C to remove MeCN, and the residue was lyophilized to give (R)-4,4,4-trifluoro-3-((2-methylene-4-(((R)-octan-2-yl)oxy)-4-oxobutanoyl)oxy)butanoic acid (134 mg, 0.35 mmol 59 %) as a colourless oil. LCMS (System 2, Method B) m / z 405.3 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 12.85 (br, 1H), 6.28 (s, 1H), 5.96 (d, J = 0.8 Hz, 1H), 5.79-5.73 (m, 1H), 4.80-4.75 (m, 1H), 3.40 (s, 2H), 2.95 (dd, J = 22.8, 4.0 Hz, 1H), 2.74 (dd, J = 16.8, 9.2 Hz, 1H), 1.48-1.33 (m, 2H), 1.29-1.23 (m, 8H), 1.12 (d, J = 6.4 Hz, 3H), 0.85(t, J = 6.4 Hz, 3H).

[0304] The following compound was synthesised using a similar procedure but starting from (S)-4,4,4-trifluoro-3-hydroxybutanoic acid: Example No. Example Structure / Name LCMS / 1< H NMR data 74 LCMS (System 2, Method B) m / z 405.3 (M+Na) +< (ES +< ).(S)-4,4,4-trifluoro-3-((2-methylene-4-(((R)-octan-2-yl)oxy)-4-oxobutanoyl)oxy)butanoic acid 1< H NMR (400 MHz, DMSO-d6) δ: 12.83 (br, 1H), 6.29 (s, 1H), 5.96 (s, 1H), 5.80-5.72 (m, 1H), 4.81-4.73 (m, 1H), 3.40 (s, 2H), 2.95 (dd, J = 22.8, 4.0 Hz, 1H), 2.74 (dd, J = 16.8, 9.2 Hz, 1H), 1.51-1.41 (m, 2H), 1.29-1.23 (m, 8H), 1.12 (d, J = 6.4 Hz, 3H), 0.85 (t, J = 6.4 Hz, 3H). Example 58 - 2-((4-(cyclooctyloxy)-3-hydroxy-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0305] Step 1

[0306] A mixture of 1-(tert-butyl) 4-cyclooctyl 3-hydroxy-2-methylenesuccinate (200 mg, 0.64 mmol) and HCl solution in 1,4-dioxane (4 M, 2 mL) in DCM (2 mL) was stirred at room temperature for 4 h.

[0307] The mixture was concentrated under reduced pressure at 40 °C to give crude 2-(chloromethyl)-4-(cyclooctyloxy)-3-hydroxy-4-oxobutanoic acid (200 mg, 0.68 mmol, >100 %) as a pale yellow oil, which was used directly in the next step. LCMS (System 2, Method C) m / z 315.2 (M+Na) +< (ES +< ).Step 2

[0308] To a solution of the crude 2-(chloromethyl)-4-(cyclooctyloxy)-3-hydroxy-4-oxobutanoic acid (200 mg, 0.68 mmol) in acetone (5 mL) was added potassium carbonate (94 mg, 0.68 mmol) and the reaction mixture was stirred at room temperature for 30 min, then tert-butyl 2-bromoacetate (158 mg, 0.82 mmol) was added, and the resulting mixture was stirred at room temperature overnight. The reaction mixture was filtered, the filtrate was concentrated under reduced pressure at 40 °C, and the residue was purified by flash column chromatography (25 g silica, 0-20% MTBE / petroleum ether) to give 1-(2-(tert-butoxy)-2-oxoethyl) 4-cyclooctyl 3-hydroxy-2-methylenesuccinate (200 mg, 0.54 mmol, 79 %) as a yellow oil. LCMS (System 2, Method B) m / z 393.3 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, CDCl 3 ) δ: 6.47 (s, 1H), 6.00 (s, 1H), 5.30 (m, 1H), 4.85 (d, J = 6.0 Hz, 1H), 4.66 (d, J = 16.0 Hz, 1H), 4.52 (d, J = 16.0 Hz, 1H), 3.56 (d, J = 6.0 Hz, 1H), 1.86-1.65 (m, 6H), 1.63-1.52 (m, 8H), 1.42 (s, 9H).Step 3

[0309] A mixture of 1-(2-(tert-butoxy)-2-oxoethyl) 4-cyclooctyl 3-hydroxy-2-methylenesuccinate (200 mg, 0.54 mmol) and TFA (2 mL) in DCM (2 mL) was stirred at room temperature overnight. The mixture was concentrated under reduced pressure at 40 °C and the residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.1% TFA / water) gradient: 35-95% MeCN; collection wavelength: 214 nm). The fractions were concentrated under reduced pressure at 40 °C to remove MeCN, and the residue was lyophilized to give 2-((4-(cyclooctyloxy)-3-hydroxy-2-methylene-4-oxobutanoyl)oxy)acetic acid (99 mg, 58 %) as a colorless oil. LCMS (System 2, Method B) m / z 337.3 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 13.10 (br, 1H), 6.30 (s, 1H), 6.07 (m, 1H), 6.03 (s, 1H), 4.85-4.79 (m, 1H), 4.83 (s, 1H), 4.64 (s, 2H), 1.71-1.53 (m, 6H), 1.49-1.36 (m, 8H).Example 59 - 2-(3-methylene-5-(4-methylheptan-4-yloxy)-5-oxopent-1-en-2-yloxy)acetic acid

[0310] Step 1

[0311] To a solution of 4-methylheptan-4-ol (1.50 g, 11.52 mmol) and DBU (2.62 g, 17.28 mmol) in 1-methyl-2-pyrrolidinone (25 mL) was slowly added 2-bromoacetyl bromide (3.49 g, 17.28 mmol) dropwise at 0 °C, and the mixture was stirred at room temperature for 1 h. The reaction mixture was diluted with water (20 mL) and MTBE (20 mL), the layers were separated and the aqueous layer was extracted with MTBE (2 x 10 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (25 g silica, 0-3% MTBE / petroleum ether) to give 4-methylheptan-4-yl 2-bromoacetate (2.00 g, 7.96 mmol, 69 %) as a colourless oil. 1< H NMR (400 MHz, CDCl 3 ) δ: 3.75 (s, 2H), 1.86-1.78 (m, 2H), 1.74-1.67 (m, 2H), 1.42 (s, 3H), 1.36-1.26 (m, 4H), 0.91 (t, J = 7.6 Hz, 6H).Step 2

[0312] To a solution of methyl 2-(diethoxyphosphoryl)acetate (1.52 g, 7.24 mmol) in THF (30 mL) was added NaH suspension in mineral oil (60 wt. %, 290 mg, 7.96 mmol) at 0 °C, and the reaction mixture was stirred at 0 °C for 0.5 h. 4-Methylheptan-4-yl 2-bromoacetate (2.00 g, 7.96 mmol) was added and the reaction mixture was stirred at room temperature overnight. The reaction mixture was then quenched with dilute aqueous HCl (0.5 M, 10 mL) to pH = 5, and extracted with EtOAc (2 x 10 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by silica gel column chromatography (25 g silica, 1:4-1:2 EtOAc / petroleum ether) to give 1-methyl 4-(4-methylheptan-4-yl) 2-(diethoxyphosphoryl)succinate (2.30 g, 6.0 mmol, 83 %) as a light yellow oil. LCMS (System 2, Method C) m / z 403.3 (M+Na) +< (ES +< ).Step 3

[0313] To a solution of 1-methyl 4-(4-methylheptan-4-yl) 2-(diethoxyphosphoryl)succinate (1.30 g, 3.42 mmmol) and potassium carbonate (945 mg, 6.84 mmol) in DMF (15 mL) was added formaldehyde solution in water (37 wt. %, 5.5 mL, 68.40 mmol) at room temperature, and the reaction mixture was stirred at room temperature for 4 h. The reaction mixture was diluted with H 2 O (30 mL) and extracted with MTBE (2 x 20 mL). The combined organic layers were washed with H 2 O (2 x 15 mL) and brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 30 °C. The residue was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give 1-methyl 4-(4-methylheptan-4-yl) 2-methylenesuccinate (600 mg, 2.34 mmol, 68 %) as a colourless oil. LCMS (System 2, Method C) m / z 279.4 (M+Na) +< (ES +< ).Step 4

[0314] To a solution of 1-methyl 4-(4-methylheptan-4-yl) 2-methylenesuccinate (600 mg, 2.34 mmol) in THF (10 mL) was added LiOH solution in water (2 M, 4.7 mL, 9.36 mmol), and the reaction mixture was stirred at room temperature overnight. The reaction mixture was acidified with dilute aqueous HCl (0.5 M) to pH = 3, and extracted with EtOAc (2 x 10 mL). The EtOAc layer was washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C to give a 5:1 mixture of 2-methylene-4-(4-methylheptan-4-yloxy)-4-oxobutanoic acid and 2-methyl-4-(4-methylheptan-4-yloxy)-4-oxobut-2-enoic acid (500 mg, 2.06 mmol, 88 %) as a pale yellow oil, which was used directly in the next step. LCMS (System 2, Method B) m / z 265.3 (M+Na) +< (ES +< ).Step 5

[0315] To a solution of the 5:1 mixture of 2-methylene-4-(4-methylheptan-4-yloxy)-4-oxobutanoic acid and 2-methyl-4-(4-methylheptan-4-yloxy)-4-oxobut-2-enoic acid (500 mg, 2.06 mmol), and potassium carbonate (313 mg, 2.26 mmol) in acetone (10 mL) was added 2,2,2-trichloroethyl 2-bromoacetate (558 mg, 2.06 mmol), and the reaction mixture was stirred at room temperature overnight. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure at 30 °C. The residue was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give 4-(4-methylheptan-4-yl) 1-(2-oxo-2-(2,2,2-trichloroethoxy)ethyl) 2-methylenesuccinate (430 mg, 1.00 mmol, 48 %) as a pale yellow oil. LCMS (System 2, Method B) m / z 455.0 (M+Na) +< (ES +< ).Step 6

[0316] To a solution of 4-(4-methylheptan-4-yl) 1-(2-oxo-2-(2,2,2-trichloroethoxy)ethyl) 2-methylenesuccinate (430 mg, 1.00 mmol) in AcOH (5 mL) was added zinc powder (325 mg, 4.98 mmol), and the reaction mixture was stirred at room temperature overnight. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure at 30 °C. The residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.1% TFA / water) gradient: 50-95% MeCN; collection wavelength: 214 nm). The fractions were concentrated under reduced pressure at 30 °C to remove MeCN, and the residue was lyophilized to give 2-(3-methylene-5-(4-methylheptan-4-yloxy)-5-oxopent-1-en-2-yloxy)acetic acid (72 mg, 0.24 mmol, 24 %) as a colourless oil. LCMS (System 2, Method B) m / z 323.2 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.23 (d, J = 1.2 Hz, 1H), 5.86 (d, J = 0.4 Hz, 1H), 4.61 (s, 2H), 3.28 (s, 2H), 1.76-1.68 (m, 2H), 1.64-1.56 (m, 2H), 1.30 (s, 3H), 1.28-1.18 (m, 4H), 0.85 (d, J = 7.2 Hz, 6H).

[0317] The following compounds were made using a similar procedure: Example No. Alcohol used in step 5 / Example Structure / Name LCMS / 1< H NMR data 601-cyclohexylcyclobutan-1-olLCMS (System 2, Method B) m / z 347.2 (M+Na) +< (ES +< ). 2-((4-(1-cyclohexylcyclobutoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.26 (d, J = 1.2 Hz, 1H), 5.90 (d, J = 0.8 Hz, 1H), 4.63 (s, 2H), 3.23 (s, 2H), 2.28-2.15 (m, 4 H), 1.88-1.80 (m, 1H), 1.79-1.68 (m, 6H), 1.63-1.61 (m, 1H), 1.54-1.45 (m, 1H), 1.20-1.06 (m, 2H), 1.02-0.92 (m, 2H).61 2-methyloctan-2-olLCMS (System 2, Method B) m / z 337.3 (M+Na) +< (ES +< ). 2-((2-methylene-4-((2-methyloctan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.11 (br, 1H), 6.24 (d, J = 0.8 Hz, 1H), 5.87 (d, J = 0.8 Hz, 1H), 4.63 (s, 2H), 3.35 (s, 2H), 1.65-1.63 (m, 2H), 1.34 (s, 6H), 1.27-1.23 (m, 8H), 0.86 (t, J = 7.2 Hz, 3H).62 2-methylheptan-2-olLCMS (System 2, Method B) m / z 323.2 (M+Na) +< (ES +< ). 2-((2-methylene-4-((2-methylheptan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.07 (br, 1H), 6.24 (d, J = 1.2 Hz, 1H), 5.87 (d, J = 0.8 Hz, 1H), 4.63 (s, 2H), 3.27 (s, 2H), 1.67-1.63 (m, 2H), 1.34 (s, 6H), 1.29-1.18 (m, 6H), 0.85 (d, J = 6.8 Hz, 3H).63 1-pentylcyclobutan-1-olLCMS (System 2, Method B) m / z 335.2 (M+Na) +< (ES +< ). 2-((2-methylene-4-oxo-4-(1-pentylcyclobutoxy) butanoyl)oxy) acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.27 (s, 1H), 5.90 (s, 1H), 4.64 (s, 2H), 3.31 (s, 2H), 2.19-2.13 (m, 2H), 2.09-2.05 (m, 2H), 1.82-1.79 (m, 2H), 1.76-1.72 (m, 1H), 1.63-1.53 (m, 1H), 1.30-1.22 (m, 6H), 0.86 (t, J = 7.2 Hz, 3H).64 2-methylspiro[3.5]nonan-2-olLCMS (System 2, Method B) m / z 347.2 (M+Na) +< (ES +< ). 2-((2-methylene-4-((2-methylspiro[3.5]nonan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.08 (br, 1H), 6.26 (s, 1H), 5.90 (s, 1H), 4.64 (s, 2H), 3.34 (s, 2H), 1.95 (q, J = 12.8 Hz, 4H), 1.46 (s, 3H), 1.40 (m, 4H), 1.31-1.30 (m, 6H).75 1-pentylcyclopropan-1-olLCMS (System 2, Method B) m / z 299.2 (M+H) +< (ES +< ). 2-((2-methylene-4-oxo-4-(1-pentylcyclopropoxy) butanoyl)oxy) acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.07 (br, 1H), 6.27 (s, 1H), 5.90 (s, 1H), 4.62 (s, 2H), 3.33 (s, 2H), 1.68-1.64 (m, 2H), 1.37-1.24 (m, 6H), 0.86 (t, J = 6.8 Hz, 3H), 0.73 (t, J = 5.2 Hz, 2H), 0.61 (t, J = 5.2 Hz, 2H).

[0318] 2-Methylspiro[3.5]nonan-2-ol was made by MeMgBr addition to the corresponding commercially available ketone in THF. 1< H NMR (400 MHz, CDCl 3 ) δ: 1.92 (d, J = 14.6 Hz, 2H), 1.89 (d, J = 14.4 Hz, 2H), 1.71 (m, 1H), 1.53-1.50 (m, 2H), 1.43-1.34 (m, 8H), 1.39 (s, 3H).Example 65 - 2-((2-methylene-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)butanoyl)oxy)acetic acid

[0319] Step 1

[0320] To a solution of (exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-ol (CAS No. 124-76-5) (1.00 g, 6.49 mmol) and DBU (1.48 g, 9.74 mmol) in 1-methyl-2-pyrrolidinone (20 mL) was slowly added 2-bromoacetyl bromide (1.97 g, 9.74 mmol) dropwise at 0 °C, and the mixture was stirred at room temperature for 3 h. The reaction mixture was diluted with water (20 mL) and MTBE (20 mL), separated and the aqueous layer was extracted with MTBE (2 x 10 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (40 g silica, 0-3% MTBE / petroleum ether) to give (exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl 2-bromoacetate (1.5 g, 5.11 mmol, 79 %) as a colourless oil. 1< H NMR (400 MHz, CDCl 3 ) δ: 4.74-4.71 (m, 1H), 3.80 (s, 2H), 1.86-1.68 (m, 4H), 1.61-1.54 (m, 1H), 1.18-1.08 (m, 2H), 1.00 (s, 3H), 0.80 (s, 3H), 0.77 (s, 3H).Step 2

[0321] To a solution of tert-butyl 2-(diethoxyphosphoryl)acetate (1.29 g, 5.11 mmol) in THF (20 mL) was added NaH suspension in mineral oil (60 wt. %, 225 mg, 5.62 mmol) at 0 °C, and the reaction mixture was stirred at 0 °C for 0.5 h, then (exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl 2-bromoacetate (1.50 g, 5.11 mmol) was added. The reaction mixture was stirred at room temperature overnight, then quenched with dilute aqueous HCl (0.5 M) to pH = 5 and extracted with EtOAc (2 x 10 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (40 g silica, 1:4-1:2 EtOAc / petroleum ether) to give 1-(tert-butyl) 4-((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) 2-(diethoxyphosphoryl)succinate (1.60 g, 3.59 mmol, 70 %) as a colourless oil. LCMS (System 2, Method C) m / z 469.4 (M+Na) +< (ES +< ).Step 3

[0322] To a mixture of 1-(tert-butyl) 4-((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) 2-(diethoxyphosphoryl)succinate (1.60 g, 3.59 mmol) and potassium carbonate (990 mg, 7.17 mmol) in DMF (10 mL) was added formaldehyde solution in water (37 wt. %, 2.9 mL, 35.9 mmol) at room temperature, and the reaction mixture was stirred at room temperature for 4 h. The reaction mixture was diluted with H 2 O (15 mL) and extracted with MTBE (2 x 20 mL). The combined organic layers were washed with H 2 O (2 x 10 mL) and brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 30 °C. The residue was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give 1-(tert-butyl) 4-((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) 2-methylenesuccinate (850 mg, 2.64 mmol, 73 %) as a colourless oil. LCMS (System 2, Method C) m / z 345.4 (M+Na) +< (ES +< ).Step 4

[0323] To a solution of 1-(tert-butyl) 4-((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) 2-methylenesuccinate (400 mg, 1.24 mmol) in DCM (8 mL) was added HCl solution in 1,4-dioxane (4 M, 4.0 mL), and the reaction mixture was stirred at room temperature overnight. The reaction mixture was concentrated under reduced pressure at 40 °C to give a crude 33:20:47 mixture of 2-methylene-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)butanoic acid (LCMS (System 2, Method C) m / z 289.4 (M+Na) +< (ES +< )), 2-methyl-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)but-2-enoic acid (LCMS (System 2, Method C) m / z 289.4 (M+Na) +< (ES +< )) and 2-(chloromethyl)-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)butanoic acid (LCMS (System 2, Method C) m / z 325.2 (M+Na) +< (ES +< )) (400 mg) as a pale yellow oil, which was used directly in the next step.Step 5

[0324] To a crude 33:20:47 mixture of 2-methylene-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy) butanoic acid, (2-methyl-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)but-2- enoic acid and 2-(chloromethyl)-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy) butanoic acid (400 mg) and potassium carbonate (342 mg, 2.48 mmol) in acetone (10 mL) was added tert-butyl 2-bromoacetate (331 mg, 1.24 mmol), and the reaction mixture was stirred at room temperature overnight. The mixture was then filtered, and the filtrate was concentrated under reduced pressure at 30 °C. The residue was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give 1-(2-oxo-2-(2,2,2-trichloroethoxy)ethyl) 4-((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) 2-methylenesuccinate (450 mg, 0.99 mmol, 80 % over two steps) as a pale yellow oil. LCMS (System 2, Method C) m / z 477.0 (M+Na) +< (ES +< ).Step 6

[0325] To a solution of 1-(2-oxo-2-(2,2,2-trichloroethoxy)ethyl) 4-((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) 2-methylenesuccinate (450 mg, 0.99 mmol) in AcOH (5 mL) was added zinc powder (322 mg, 4.96 mmol), and the reaction mixture was stirred at room temperature overnight. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure at 30 °C. The residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.05% TFA / water) gradient: 40-95% MeCN; collection wavelength: 214 nm). The fractions were concentrated under reduced pressure at 30 °C to remove MeCN, and the residue was lyophilized to give 2-((2-methylene-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) oxy)butanoyl)oxy)acetic acid (77 mg, 0.23 mmol, 24 %) as a colourless oil. LCMS (System 2, Method B) m / z 347.2 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ: 13.06 (br, 1H), 6.27 (d, J = 0.8 Hz, 1H), 5.92 (d, J = 0.8 Hz, 1H), 4.61 (s, 2H), 4.54 (dd, J = 8.0, 3.6 Hz, 1H), 3.35 (s, 2H), 1.74-1.60 (m, 4H), 1.53-1.47 (m, 1H), 1.13-1.01 (m, 2H), 0.88 (s, 3H), 0.79 (s, 3H), 0.75 (s, 3H).

[0326] The following compound was made using a similar procedure: Example No. / Structure / Name Alcohol / Example LCMS / 1< H NMR data 66 2,2,6,6-tetramethylcyclohexan-1-olLCMS (System 2, Method B) m / z 349.3 (M+Na) +< (ES +< ). 2-((2-methylene-4-oxo-4-((2,2,6,6-tetramethylcyclohexyl)oxy) butanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.09 (br, 1H), 6.31 (d, J = 1.2 Hz, 1H), 5.98 (s, 1H), 4.62 (s, 2H), 4.40 (s, 1H), 3.46 (s, 2H), 1.56-1.44 (1H, m), 1.45-1.37 (2H, m), 1.37-1.29 (m, 1H), 1.27-1.16 (m, 2H), 0.86 (s, 6H), 0.75 (s, 6H).

[0327] 2,2,6,6-Tetramethylcyclohexan-1-ol was made by reduction of the corresponding commercially available ketone with NaBH 4 in MeOH. 1< H NMR (400 MHz, DMSO-d6) δ: 4.40 (br, 1H), 2.83 (s, 1H), 1.56-1.48 (1H, m), 1.47-1.38 (m, 2H), 1.36-1.28 (m, 1H), 1.22-1.11 (m, 2H), 0.93 (s, 6H), 0.88 (s, 6H).

[0328] The following examples were prepared according to the procedure of Example 23, but using the alcohols described below instead of 3-ethoxypropan-1-ol: Example No. Alcohol / Example Structure / Name LCMS / 1< H NMR data 67 1-(3,5-dichlorophenyl)ethan-1-ol (Isomer 1)LCMS (System 2, Method B) m / z 383.0 (M+Na) +< (ES +< ). 2-((4-(1-(3,5-dichlorophenyl)ethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid (Isomer 1) 1< H NMR (400 MHz, DMSO-d6) δ 13.12 (br, 1H), 7.54 (t, J = 2.0 Hz, 1H), 7.41 (d, J = 1.6 Hz, 2H), 6.31 (d, J = 0.8 Hz, 1H), 5.96 (d, J = 0.8 Hz, 1H), 5.77 (q, J = 6.4 Hz, 1H), 4.63 (s, 2H), 3.47 (s, 2H), 1.44 (d, J = 6.4 Hz, 3H).68 1-(3,5-dichlorophenyl)ethan-1-ol (Isomer 2)LCMS (System 2, Method B) m / z 383.0 (M+Na) +< (ES +< ). 2-((4-(1-(3,5-dichlorophenyl)ethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid (Isomer 2) 1< H NMR (400 MHz, DMSO-d6) δ 13.12 (br, 1H), 7.54 (t, J = 2.0 Hz, 1H), 7.41 (d, J = 1.6 Hz, 2H), 6.31 (d, J = 0.8 Hz, 1H), 5.96 (d, J = 0.8 Hz, 1H), 5.77 (q, J = 6.4 Hz, 1H), 4.63 (s, 2H), 3.47 (s, 2H), 1.44 (d, J = 6.4 Hz, 3H).69 (R)-1-(4-(trifluoromethyl)phenyl) ethan-1-ol)LCMS (System 2, Method B) m / z 383.0 (M+Na) +< (ES +< ). (R)-2-((2-methylene-4-oxo-4-(1-(4-(trifluoromethyl)phenyl)ethoxy)butanoyl) oxy) acetic acid) 1< H NMR (400 MHz, DMSO-d6) δ 13.13 (br, 1H), 7.73 (d, J = 8.4 Hz, 2H), 7.57 (d, J = 8.4 Hz, 2H), 6.30 (d, J = 0.8 Hz, 1H), 5.94 (d, J = 0.8 Hz, 1H), 5.86 (q, J = 6.8 Hz, 1H), 4.62 (s, 2H), 3.46 (s, 2H), 1.47 (d, J = 6.4 Hz, 3H).70 (S)-1-(4-(trifluoromethyl)phenyl) ethan-1-ol)LCMS (System 2, Method B) m / z 383.0 (M+Na) +< (ES +< ). (S)-2-((2-methylene-4-oxo-4-(1-(4-(trifluoromethyl)phenyl)ethoxy)butanoyl) oxy) acetic acid) 1< H NMR (400 MHz, DMSO-d6) δ 13.06 (br, 1H), 7.73 (d, J = 8.4 Hz, 2H), 7.57 (d, J = 8.4 Hz, 2H), 6.30 (d, J = 0.8 Hz, 1H), 5.94 (d, J = 0.8 Hz, 1H), 5.86 (q, J = 6.8 Hz, 1H), 4.62 (s, 2H), 3.46 (s, 2H), 1.47 (d, J = 6.4 Hz, 3H).72 (S)-1-cyclohexylethan-1-olLCMS (System 2, Method B) m / z 321.3 (M+Na) +< (ES +< ). (S)-2-((4-(1-cyclohexylethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid 1< H NMR (400 MHz, DMSO-d6) δ: 13.14 (br, 1H), 6.28 (s,1H), 5.92 (s, 1H), 4.62 (s, 2H), 4.66-4.58 (m, 1H), 3.26 (s, 2H), 1.69-1.67 (m, 3H), 1.62-1.53 (m, 2H), 1.42-1.35 (m, 1H), 1.25-1.08 (m, 3H), 1.15 (d, J = 6.4 Hz, 3H), 1.04-0.82 (m, 2H).

[0329] 1-(3,5-dichlorophenyl)ethan-1-ol (Isomer 1) was prepared by the following method: Racemic 1-(3,5-dichlorophenyl)ethan-1-ol was resolved using chiral SFC (Column: CHIRALPAK AY-3 4.6x100 mm; Flow Rate: 2 mL / min; solvent system: 10% IPA / CO 2 ; collection wavelength: 214 nm). Isomer 1 was the first eluting peak at 1.34 min.

[0330] 1-(3,5-dichlorophenyl)ethan-1-ol (Isomer 2) was prepared by the following method: Racemic 1-(3,5-dichlorophenyl)ethan-1-ol was resolved using chiral SFC (Column: CHIRALPAK AY-3 4.6x100 mm; Flow Rate: 2 mL / min; solvent system: 10% IPA / CO 2 ; collection wavelength: 214 nm). Isomer 2 was the second eluting peak at 1.53 min.Example 71 - 2-((4-(1-cyclohexylcyclopropoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid

[0331] Step 1

[0332] To a solution of methyl cyclohexanecarboxylate (4.26 g, 30 mmol) and titanium tetraisopropoxide (11.93 g, 42 mmol) in THF (60 mL) at 0 °C was slowly added a solution of ethyl magnesium bromide in diethyl ether (3M, 30 mL, 90 mmol), and the mixture was stirred at room temperature for 16 h. The reaction mixture was quenched with water (60 mL), and stirred for 1 h until a gray precipitate was formed, and then filtered. The filtrate was extracted with MTBE (3 x 40 mL), and the combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (0-3% EtOAc / petroleum ether) give 1-cyclohexylcyclopropan-1-ol (2.50 g, 17.8 mmol, 60 %) as a colourless oil. 1< H NMR (400 MHz, CDCl 3 ) δ: 1.80-1.75 (m, 4H), 1.69-1.66 (m, 1H), 1.26-1.17 (m, 5H), 0.94-0.92 (m, 1H), 0.70-0.67 (m, 2H), 0.45-0.42 (m, 2H). One exchangeable proton not observed.Step 2

[0333] To a solution of 1-cyclohexylcyclopropan-1-ol (1.50 g, 10.7 mmol) and DBU (2.43 g, 16.05 mmol) in 1-methyl-2-pyrrolidinone (20 mL) was slowly added 2-bromoacetyl bromide (3.24 g, 16.05 mmol) dropwise at 0 °C, and the resulting mixture was stirred at room temperature for 1 h. The reaction mixture was diluted with water (20 mL) and MTBE (20 mL), separated and the aqueous layer was extracted with MTBE (2 x 10 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (25 g silica, 0-3% MTBE / petroleum ether) to give 1-cyclohexylcyclopropyl 2-bromoacetate (1.70 g, 6.51 mmol, 61 %) as a colourless oil. 1< H NMR (400 MHz, CDCl 3 ) δ: 3.74 (s, 2H), 1.80-1.73 (m, 5H), 1.67-1.64 (m, 1H), 1.24-1.08 (m, 3H), 0.95-0.79(m, 2H), 0.70-0.67 (m, 4H).Step 3

[0334] To a solution of methyl 2-(diethoxyphosphoryl)acetate (1.36 g, 6.51 mmol) in THF (30 mL) was added NaH suspension in mineral oil (60 wt. %, 261 mg, 6.51 mmol) at 0 °C, and the reaction mixture was stirred at 0 °C for 0.5 h. Then 1-cyclohexylcyclopropyl 2-bromoacetate (1.70 g, 6.51 mmol) was added, and the reaction mixture was stirred at room temperature overnight. The reaction mixture was quenched with dilute aqueous HCl (0.5 M) to pH = 5, and extracted with EtOAc (2 x 10 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C to give 4-(1-cyclohexylcyclopropyl) 1-methyl 2-(diethoxyphosphoryl)succinate (2.70 g, 6.92 mmol, > 100 %) as a colourless oil. The crude product was used directly in next step. LCMS (System 2, Method B) m / z 413.2 (M+Na) +< (ES +< ).Step 4

[0335] To a mixture of 4-(1-cyclohexylcyclopropyl) 1-methyl 2-(diethoxyphosphoryl)succinate (2.70 g, 6.92 mmmol) and potassium carbonate (1.83 g, 13.8 mmol) in THF (20 mL) was added formaldehyde solution in water (37 wt. %, 11.2 mL, 138 mmol) at room temperature, and the reaction mixture was stirred at room temperature for 4 h. The reaction mixture was diluted with H 2 O (20 mL) and extracted with MTBE (2 x 20 mL). The combined organic layers were washed with H 2 O (2 x 15 mL) and brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 30 °C. The residue was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give 4-(1-cyclohexylcyclopropyl) 1-methyl 2-methylenesuccinate (1.20 g, 4.51 mmol, 65 %) as a colourless oil. LCMS (System 2, Method B) m / z 267.3 (M+H) +< (ES +< ).Step 5

[0336] To a solution of 4-(1-cyclohexylcyclopropyl) 1-methyl 2-methylenesuccinate (600 mg, 2.25 mmol) in THF (8 mL) was added LiOH solution in water (2 M, 3.4 mL, 6.75 mmol), and the reaction mixture was stirred at room temperature for 7 h (about 24 % of starting material remained). The reaction mixture was acidified with dilute aqueous HCl (0.5 M) to pH = 3 and extracted with EtOAc (2 x 10 mL). The EtOAc layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C to give a 2:1 mixture of 4-(1-cyclohexylcyclopropoxy)-2-methylene-4-oxobutanoic acid and 4-(1-cyclohexylcyclopropoxy)-2-methyl-4-oxobut-2-enoic acid (500 mg, 1.98 mmol, 88 %) as a pale yellow oil, which was used directly in the next step. LCMS (System 2, Method C) m / z 253.4 (M+H) +< (ES +< ).Step 6

[0337] To a solution of a 2:1 mixture of 4-(1-cyclohexylcyclopropoxy)-2-methylene-4-oxobutanoic acid and 4-(1-cyclohexylcyclopropoxy)-2-methyl-4-oxobut-2-enoic acid (500 mg, 1.98 mmol), and potassium carbonate (328 mg, 2.38 mmol) in acetone (10 mL) was added 2,2,2-trichloroethyl 2-bromoacetate (530 mg, 1.98 mmol), and the reaction mixture was stirred at room temperature overnight. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure at 30 °C. The residue was purified by flash column chromatography (25 g silica, 0-10% MTBE / petroleum ether) to give 4-(1-cyclohexylcyclopropyl) 1-(2-oxo-2-(2,2,2-trichloroethoxy)ethyl) 2-methylenesuccinate (300 mg, 0.68 mmol, 34 %) as a pale yellow oil. LCMS (System 2, Method B) m / z 463.1 (M+Na) +< (ES +< ).Step 7

[0338] To a solution of 4-(1-cyclohexylcyclopropyl) 1-(2-oxo-2-(2,2,2-trichloroethoxy)ethyl) 2-methylenesuccinate (100 mg, 0.22 mmol) in THF (2 mL) and water (0.5 mL) was added zinc powder (71 mg, 1.10 mmol) and sodium acetate (90 mg, 1.10 mmol), and the reaction mixture was stirred at room temperature overnight. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure at 30 °C. The residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.05% TFA / water) gradient: 30-95% MeCN; collection wavelength: 214 nm). The fractions were concentrated under reduced pressure at 30 °C to remove MeCN, and the residue was lyophilized to give 2-((4-(1-cyclohexylcyclopropoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid (6 mg, 0.019 mmol, 9 %) as a colourless oil. LCMS (System 2, Method B) m / z 311.2 (M+H) +< (ES +< ). 1< H NMR (400 MHz, CDCl 3 ) δ: 6.43 (s, 1H), 5.80 (s, 1H), 4.74 (s, 2H), 3.31 (s, 2H), 1.79-1.71 (m, 5H), 1.66-1.63 (m, 1H), 1.25-1.15 (m, 2H), 1.11-1.05 (m, 1H), 0.92-0.83 (m, 2H), 0.74 (d, J = 2.0 Hz, 4H). One exchangeable proton not observed.Example 73 - 2-((2-methylene-4-oxo-4-((2,2,4,4-tetramethylpentan-3-yl)oxy)butanoyl) oxy)acetic acid

[0339] Step 1

[0340] To a solution of 2,2,4,4-tetramethylpentan-3-one (1.5 g, 10.6 mmol) in dry THF (40 mL) was added LiAlH 4 (802 mg, 21.1 mmol) at 0 °C, and the resulting mixture was stirred at room temperature for 1 h. To the reaction mixture was sequentially added water (1 mL), aqueous NaOH (15 wt %, 1 mL), water (2.5 mL) and Na 2 SO 4 (20 g), the mixture was stirred at room temperature for 20 min, filtered, and concentrated under reduced pressure at 35 °C to give 2,2,4,4-tetramethylpentan-3-ol (1.4 g, 9.70 mmol, 90 % ) as colourless crystals. 1< H NMR (400 MHz, CDCl 3 ) δ: 3.12 (d, J= 2.4 Hz, 1H), 1.23 (s, 18H). One exchangeable proton not observed.Step 2

[0341] To a solution of 2,2,4,4-tetramethylpentan-3-ol (1.3 g, 9.02 mmol) and DBU (2.74 g, 18.0 mmol) in 1-methyl-2-pyrrolidinone (45 mL) was slowly added 2-bromoacetyl bromide (3.64 g, 18.0 mmol) at 0 °C dropwise and the mixture was stirred at room temperature for 1 h. The reaction mixture was diluted with water (10 mL) and MTBE (20 mL), separated and the aqueous layer was extracted with MTBE (2 x 20 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (50 g silica, 0-10% EtOAc / petroleum ether) to give 2,2,4,4-tetramethylpentan-3-yl 2-bromoacetate (1.50 g, 5.68 mmol, 62 %) as a colorless oil. 1< H NMR (400 MHz, CDCl 3 ) δ: 4.63 (s, 1H), 3.87 (s, 2H), 1.04 (s, 18H).Step 3

[0342] To a solution of tert-butyl 2-(diethoxyphosphoryl)acetate (1.43 g, 5.68 mmol) in THF (15 mL) was added NaH suspension in mineral oil (60 wt. %, 227 mg, 5.68 mmol) at 0 °C, and the reaction mixture was stirred at 0 °C for 0.5 h. 2,2,4,4-Tetramethylpentan-3-yl 2-bromoacetate (1.5 g, 5.68 mmol) was then added and the reaction mixture was stirred at room temperature overnight. The reaction mixture was quenched with dilute aqueous HCl (0.5 M) to pH = 5, and extracted with EtOAc (3 x 20 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C to give crude 1-(tert-butyl) 4-(2,2,4,4-tetramethylpentan-3-yl) 2-(diethoxyphosphoryl)succinate (3.00 g, 6.87 mmol, > 100 %) as a colourless oil. The crude product was used directly in next step. LCMS (System 2, Method B) m / z 459.3 (M+Na) +< (ES +< ).Step 4

[0343] To a solution of 1-(tert-butyl) 4-(2,2,4,4-tetramethylpentan-3-yl) 2-(diethoxyphosphoryl)succinate (3.00 g, ~ 6.87 mmol, crude) and potassium carbonate (1.90 g, 13.8 mmol) in THF (24 mL) and H 2 O (6 mL) was added formaldehyde solution in water (37 wt. %, 11.15 mL, 138 mmol) at room temperature, and the reaction mixture was stirred at room temperature for 4 h. The reaction mixture was diluted with H 2 O (15 mL) and extracted with MTBE (3 x 30 mL). The combined organic layers were washed with H 2 O (2 x 10 mL) and brine, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (25 g silica, 0-20% MTBE / petroleum ether) to give 1-(tert-butyl) 4-(2,2,4,4-tetramethylpentan-3-yl) 2-methylenesuccinate (1.45 g, 4.64 mmol, 68 %) as a colourless oil. LCMS (System 2, Method B) m / z 335.4 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, CDCl 3 ) δ: 6.24 (d, J = 1.2 Hz, 1H), 5.63 (d, J = 1.2 Hz, 1H), 4.57 (s, 1H), 3.35 (d, J = 0.8 Hz, 2H), 1.48 (s, 9H), 0.98 (s, 18H).Step 5

[0344] A solution of 1-(tert-butyl) 4-(2,2,4,4-tetramethylpentan-3-yl) 2-methylenesuccinate (450 mg, 1.44 mmol) in TFA / DCM (2:1, 7 mL) was stirred at room temperature for 4 h. The mixture was concentrated under reduced pressure at 40 °C to give 2-methylene-4-oxo-4-((2,2,4,4-tetramethylpentan-3-yl)oxy)butanoic acid (400 mg, 1.56 mmol, > 100 %) as a colourless oil, which was used directly in the next step. LCMS (System 2, Method C) m / z 279.4 (M+Na) +< (ES +< ).Step 6

[0345] To a solution of 2-methylene-4-oxo-4-((2,2,4,4-tetramethylpentan-3-yl)oxy)butanoic acid (400 mg, 1.56 mmol), and potassium carbonate (645 mg, 4.68 mmol) in acetone (10 mL) was added tert-butyl 2-bromoacetate (608 mg, 3.12 mmol), and the reaction mixture was stirred at room temperature for 2 days. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure at 40 °C. The residue was purified by flash column chromatography (25 g silica, 0-20% MTBE / petroleum ether) to give 1-(2-(tert-butoxy)-2-oxoethyl) 4-(2,2,4,4-tetramethylpentan-3-yl) 2-methylenesuccinate (430 mg, 1.16 mmol, 81 %) as a colourless oil. LCMS (System 2, Method C) m / z 393.4 (M+Na) +< (ES +< ).Step 7

[0346] A solution of 1-(2-(tert-butoxy)-2-oxoethyl) 4-(2,2,4,4-tetramethylpentan-3-yl) 2-methylenesuccinate (430 mg, 1.16 mmol) in TFA / DCM (2:1, 6 mL) was stirred at room temperature for 1 h. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure at 40 °C. The residue was purified by preparative HPLC (Column: Waters X-Bridge C18 OBD 10µm 19x250 mm; Flow Rate: 20 mL / min; solvent system: MeCN / (0.05% TFA / water) gradient: 50-95% MeCN; collection wavelength: 214 nm). The fractions were concentrated under reduced pressure at 40 °C to remove MeCN, and the residue was lyophilized to give 2-((2-methylene-4-oxo-4-((2,2,4,4-tetramethylpentan-3-yl)oxy)butanoyl)oxy)acetic acid (302 mg, 0.96 mmol, 83 %) as a white solid. LCMS (System 2, Method B) m / z 337.3 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, CDCl 3 ) δ: 13.01 (br, 1H), 6.30 (d, J = 1.2 Hz, 1H), 5.97 (s, J = 1.2 Hz, 1H), 4.62 (s, 2H), 4.45 (s, 1H), 3.45 (s, 2H), 0.93 (s, 18H).Example 77 - 3-((2-methylene-4-oxo-4-(2,2,4,4-tetramethylcyclobutoxy)butanoyl)oxy) propanoic acid

[0347]

[0348] Example 77 was prepared according to the procedure of Example 3, but using 2,2,4,4-tetramethylcyclobutan-1-ol instead of 4-octyl itaconate. LCMS (System 2, Method B) m / z 335.2 (M+Na) +< (ES +< ). 1< H NMR (400 MHz, DMSO-d6) δ 12.41 (br, 1H), 6.17 (d, J = 1.2 Hz, 1H), 5.85 (d, J = 0.8 Hz, 1H), 4.36 (s, 1H), 4.25 (t, J = 2.0 Hz, 2H), 3.39 (s, 2H), 2.59 (t, J = 2.0 Hz, 2H), 1.51 (d, J = 11.6 Hz, 1H), 1.41 (d, J = 11.6 Hz, 1H), 1.10 (s, 6H), 1.00 (s, 6H).Biological Example 1 - THP-1 AlphaLISA IL-1β and IL-6 Cytokine Assay Measuring inhibitory effects on IL-1β and IL-6 cytokine output from THP-1s

[0349] The cytokine inhibition profiles of compounds of formula (I) were determined in a differentiated THP-1 cell assay. All assays were performed in RPMI-1640 growth medium (Gibco), supplemented with 10% fetal bovine serum (FBS; Gibco), 1% penicillin-streptomycin and 1% sodium pyruvate unless specified otherwise. The IL-1β and IL-6 cytokine inhibition assays were each run in a background of differentiated THP-1 cells as described below. All reagents described were from Sigma-Aldrich unless specified otherwise. Compounds were prepared as 10mM DMSO stocks.Assay Procedure

[0350] THP-1 cells were expanded as a suspension up to 80% confluence in appropriate growth medium. Cells were harvested, suspended, and treated with an appropriate concentration of phorbol 12-myristate 13-acetate (PMA) over a 72hr period (37°C / 5% CO 2 ).

[0351] Following 72hrs of THP-1 cell incubation, cellular medium was removed and replaced with fresh growth media containing 1% of FBS. Working concentrations of compounds were prepared separately in 10% FBS treated growth medium and pre-incubated with the cells for 30 minutes (37°C / 5% CO 2 ). Following the 30 minute compound pre-incubation, THP-1s were treated with an appropriate concentration of LPS and the THP-1s were subsequently incubated for a 24hr period (37°C / 5% CO 2 ). An appropriate final concentration of Nigericin was then dispensed into the THP-1 plates and incubated for 1 hour (37°C / 5% CO 2 ) before THP-1 supernatants were harvested and collected in separate polypropylene 96-well holding plates.

[0352] Reagents from each of the IL-1β and IL-6 commercial kits (Perkin Elmer) were prepared and run according to the manufacturer's instructions. Subsequently, fluorescence signal detection in a microplate reader was measured (EnVision ®< Multilabel Reader, Perkin Elmer).

[0353] Percentage inhibition was calculated per cytokine by normalising the sample data to the high and low controls used within each plate (+ / - LPS respectively). Percentage inhibition was then plotted against compound concentration and the 50% inhibitory concentration (IC 50 ) was determined from the resultant concentration-response curve.

[0354] A number of Example compounds of formula (I) were tested and the results are shown in Table 1 below. Dimethyl itaconate and dimethyl fumarate were included as comparator compounds. All compounds of formula (I) shown in Table 1 exhibited comparable or improved cytokine-lowering potencies compared to dimethyl itaconate and / or dimethyl fumarate for IL-1β and / or IL-6. Certain compounds shown in Table 1 exhibited improved cytokine-lowering potencies compared to dimethyl itaconate and / or dimethyl fumarate for IL-1β and / or IL-6. Table 1 - THP-1 cell IL-1β and IL-6 IC 50 values (µM)Compound IL-1β (IC 50 ) IL-6 (IC 50 ) dimethyl fumarate14.49.3dimethyl itaconate>100100.0Example 17.25.5Example 211.913.5Example 39.65.3Example 410052.1Example 525.718.9Example 634.296.9Example 714.322Example 822.618.6Example 910015.7Example 1028.1100Example 1125.220.4Example 1217.512.6Example 1316.49.8Example 1448.133.8Example 1575.321.2Example 1673.828.1Example 175.3NT*Example 1880.6NT*Example 1915.747.1Example 2010070.9Example 2114.6NT*Example 2210048.5Example 2310056.2Example 2459.536.3Example 2510060.4Example 2654.2NT*Example 2713NT*Example 281.7NTExample 2919.3NTExample 308.5NTExample 3113.4NTExample 3229.6NTdimethyl fumarate14.49.3dimethyl itaconate>100100.0Example 3312.1NTExample 3411.7NTExample 3510.3NTExample 3610.3NTExample 377.0NTExample 388.4NTExample 3916.5NTExample 4049.3NTExample 4115.6NTExample 4214.7NTExample 4321.8NTExample 4423.8NTExample 4527.6NTExample 4610.9NTExample 477.6NTExample 4812.0NTExample 4920.5NTExample 5024.6NTExample 5111.0NTExample 528.2NTExample 5332.8NTExample 546.1NTExample 559.6NTExample 5633.9NTExample 573.5NTExample 5857.9NTExample 5958.4NTExample 6026.6NTExample 6110.6NTExample 6217.6NTExample 6324.8NTExample 6424.3NTExample 6518.8NTdimethyl fumarate14.49.3dimethyl itaconate>100100.0Example 6621.4NTExample 6722.0NTExample 6818.3NTExample 6911.5NTExample 7024.6NTExample 7136.7NTExample 7222.0NTExample 7341.7NTExample 741.7NTExample 7519.1NTExample 77100NTNT* = not tested Biological Example 2 - Primary human monocyte AlphaLISA IL-1β and IL-6 Cytokine Assay Measuring inhibitory effects on IL-1β and IL-6 cytokine output from isolated primary human monocytes

[0355] The cytokine inhibition profiles of compounds of formula (I) were determined in a CD14 +< isolated primary human monocyte cell assay. All assays were performed in RPMI-1640 growth medium (Gibco), supplemented with heat-inactivated fetal bovine serum (FBS) and 1% penicillin-streptomycin unless specified otherwise. The IL-1β and IL-6 cytokine inhibition assays were each run in a background of isolated primary human monocyte cells as described below. All reagents described were from Sigma-Aldrich unless specified otherwise. Compounds were prepared as 100mM DMSO stocks.Assay Procedure

[0356] Primary peripheral blood mononuclear cells (PBMCs) were isolated from human whole blood. Following PBMC isolation, a CD14 +< monocyte isolation step was conducted whereby CD14 +< magnetic beads (Miltenyi Biotec) were incubated for 15 minutes with the PBMC suspension that was previously treated with ice cold T-cell isolation buffer (PBS, 0.5% BSA, 2mM EDTA). Following bead incubation, the treated cell suspension was passed through a magnetic separation column designed to positively select for magnetically labelled cells. The isolated CD14 +< monocytes were subsequently plated at the appropriate cell density for the assay, prior to compound treatment on the day of plating. Working concentrations of compounds were prepared separately in RPMI-1640 only growth medium and pre-incubated with the cells for 30 minutes (37°C / 5% CO 2 ). Following the 30 minute compound pre-incubation, primary monocytes were treated with an appropriate concentration of LPS and subsequently incubated for a 24hr period (37°C / 5% CO 2 ). An appropriate final concentration of Nigericin was then dispensed into the primary monocyte plates and incubated for 1 hour (37°C / 5% CO 2 ) before monocyte supernatants were harvested and collected in separate polypropylene 96-well holding plates prior to commencing the AlphaLISA cytokine assay.

[0357] Reagents from each of the IL-1β and IL-6 commercial kits (Perkin Elmer) were prepared and run according to the manufacturer's instructions. Subsequently, fluorescence signal was measured in a microplate reader (EnVision ®< Multilabel Reader, Perkin Elmer).

[0358] Percentage inhibition was calculated per cytokine by normalising the sample data to the high and low controls used within each plate (+ / - LPS respectively). Percentage inhibition was then plotted against compound concentration and the 50% inhibitory concentration (IC 50 ) was determined from the resultant concentration-response curve.

[0359] A number of Example compounds of formula (I) were tested and the results are shown in Table 2 below. Dimethyl fumarate and 4-octyl itaconate were included as comparator compounds. All compounds which were tested exhibited lower IC 50s than 4-octyl itaconate. Table 2 - primary monocyte IL-1β and IL-6 IC 50 values (µM)Compound IL-1β (IC 50 ) IL-6 (IC 50 ) dimethyl fumarate8.515.74-octyl itaconate>100NT*Example 1117.3NTExample 1381.3NTExample 2113.7NTNT* = not tested Biological Example 3 - NRF2 + / - GSH activation assay Measuring compound activation effects on the anti-inflammatory transcription factor NRF2 in DiscoverX PathHunter NRF2 translocation kit

[0360] Potency and efficacy of compounds of formula (I) against the target of interest to activate NRF2 (nuclear factor erythroid 2-related factor 2) were determined using the PathHunter NRF2 translocation kit (DiscoverX). The NRF2 translocation assay was run using an engineered recombinant cell line, utilising enzyme fragment complementation to determine activation of the Keap1-NRF2 protein complex and subsequent translocation of NRF2 into the nucleus. Enzyme activity was quantified using a chemiluminescent substrate consumed following the formation of a functional enzyme upon PK-tagged NRF2 translocation into the nucleus.

[0361] The assay was run under either + / - GSH (glutathione) conditions to determine the attenuating activities of GSH against target compounds.

[0362] Additionally, a defined concentration of dimethyl fumarate was used as the 'High' control to normalise test compound activation responses to.Assay Procedure

[0363] U2OS PathHunter eXpress cells were thawed from frozen prior to plating. Following plating, U2OS cells were incubated for 24hrs (37°C / 5%CO 2 ) in commercial kit provided cell medium.

[0364] Following 24hrs of U2OS incubation, cells were directly treated with an appropriate final concentration of compound, for -GSH conditions, or for +GSH conditions, an intermediate plate containing 6x working concentrations of compound stocks was prepared in a 6mM working concentration of GSH solution (solubilised in sterile PBS). Following a 30 minute compound-GSH pre-incubation (37°C / 5%CO 2 ) for +GSH treatment, plated U2OS cells were incubated with an appropriate final concentration of compound and GSH.

[0365] Following compound (+ / -GSH) treatment, the U2OS plates were incubated for a further 6 hours (37°C / 5%CO 2 ) before detection reagent from the PathHunter NRF2 commercial kit was prepared and added to test plates according to the manufacturer's instructions. Subsequently, the luminescence signal detection in a microplate reader was measured (PHERAstar ®< , BMG Labtech).

[0366] Percentage activation was calculated by normalising the sample data to the high and low controls used within each plate (+ / - DMF). Percentage activation / response was then plotted against compound concentration and the 50% activation concentration (EC 50 ) was determined from the plotted concentration-response curve.

[0367] A number of compounds of formula (I) were tested, and the results are shown in Table 3 below. Dimethyl itaconate and dimethyl fumarate were included as comparator compounds. Certain compounds of formula (I) shown in Table 3 exhibited a lower or comparable EC 50 and / or a higher E max compared with dimethyl itaconate and / or dimethyl fumarate. Table 3 - NRF2 activationCompound EC 50 (µM) E max (%) dimethyl fumarate5.6102dimethyl itaconate21.4137Example 115.8232Example 321.5229Example 424.9110Example 526.5164Example 76.3185Example 825.0163Example 921.091Example 1034.2113Example 113.2139Example 1222.4238Example 1329.4157Example 1410.173Example 1537.8109Example 1636.0118Example 1722.2193Example 1836.5122Example 1921.2180Example 218.9173Example 2717.5178Example 282.1176Example 29>1007Example 5014.3188dimethyl fumarate5.6102dimethyl itaconate21.4137Example 545.5188Example 5818.0137Example 6111.8194 Biological Example 4 - Mouse pharmacokinetic studies

[0368] Pharmacokinetic studies were carried out in 6-8-week-old male C57BL / 6 mice having free access to food and water. Intravenous dosing was conducted at 10 mg / kg (5 mL / kg; vehicle: 10% DMSO-90% (25% HP-β-CD in water)) via tail vein injection, with sampling at 3min, 8min, 15min, 30min, 1, 2, 4, 6 and 8 hours, i.e., 9 time points in total (N=3 / time point), using semi-serial bleeding for plasma. Oral compound administration, via gavage, was carried out at 100 mg / kg (10 mL / kg; vehicle: 5% DMSO-95% (0.5% HPMC + 0.1% Tween 80 in water)), with sampling at 5min, 15min, 30min, 1, 2, 4, 6, 8 and 24 hours, i.e., 9 time points in total (N=3 / time point), using semi-serial bleeding for plasma.

[0369] For both intravenous and oral routes, the mice were restrained manually at the designated time points, with ca. 110 µL of blood being taken into K2EDTA tubes via the facial vein. Blood samples were put on ice and centrifuged to obtain plasma samples from which the concentration at each time point was measured by LC-MS / MS.

[0370] A number of compounds of formula (I) were tested, and the results are shown in Tables 4 and 5 below. Dimethyl itaconate was included as a comparator compound. All compounds of formula (I) exhibited higher systemic exposures than dimethyl itaconate which was only quantifiable at one timepoint following intravenous dosing and was below the limit of quantification at all timepoints following oral administration. Table 4 - Parent compound concentrations (ng / mL) following intravenous administrationTime (h) Compound 0.05 0.133 0.25 0.5 1 2 4 6 8 dimethyl itaconate874<24<24<24<24<24<24<24<24Example 51760083472783752692.21.41.6<0.8Example 75575-*1034217346.5<2.0<2.0-Example 114250023533123676253251320040114.2Example 12278331237776802300430656.8<4.0<4.0Example 1319233716020231597893.62.81.71.2Example 171800060732437550421.91.4<0.8<0.8Example 191510035876591696.43.0<0.8<0.8<0.8Example 21634674013336233235331300035371513424154Example 4115600643328035168.62.51.3<0.8<0.8Example 423716721867893316634766612<4.0<4.0Example 44236671130026371211759.96.1<4.0<4.0Example 4727467117006110119318132<4.0<4.0<4.0Example 50333671550056772850594618.2<4.0<4.0Example 51178675317181045613<4.0<4.0<4.0<4.0Example 5440833179336737289364288206.0<4.0Example 6136633214331127329074008626<4.0<4.0Example 62288331067740401450134164.1<4.0<4.0*Timepoints where no samples were taken are indicated with a dash, "-". Table 5 - Parent compound concentrations (ng / mL) following oral administration Time (h) Compound 0.083 0.25 0.5 1 2 4 6 8 24 Dimethyl itaconate<24<24<24<24<24<24<24<24<24Example 1-*53684334411112-4.5<1.5Example 3-850115171517367-<1.5<1.5Example 5275331800064473400104256811286<0.8Example 7-9089511633141005368-217<31Example 9-4194368614919-<3.0<3.0Example 10-353331588485151465198-31<14Example 11539004363333767126831052741051947725.5Example 12439673913328500139674737918582166.8Example 132286710637620033733473164510485001.1Example 14-88636235192045033-1913Example 17278331256382173113213739219690<0.8Example 1915300477337801551810856330443<0.8Example 2179833645677233349500314332126767831218725Example 4137800987746031750971215577.7<0.8Example 425500038367405671005344204403441258.1Example 47391331946718233551714072000784231<4.0Example 6067067270672793311050366014642245639<4.0Example 54658674683327867134935393137212854175.9Example 61470006066737900251678737544079416.6Example 625290045600315338927212392055195.6 *Timepoints where no samples were taken are indicated with a dash, "-".

[0371] These results reveal that compounds of the invention display improved systemic exposures, as shown by the plasma concentrations of certain compounds of formula (I) in this assay. All the compounds of formula (I) shown in Table 4 exhibited higher systemic exposures compared with dimethyl itaconate when administered intravenously, and all the compounds of formula (I) shown in Table 5 exhibited higher systemic exposures compared with dimethyl itaconate when administered orally.Biological Example 5 - Hepatocyte stability assay

[0372] Defrosted cryo-preserved hepatocytes (viability > 70%) are used to determine the metabolic stability of a compound via calculation of intrinsic clearance (Cl int ; a measure of the removal of a compound from the liver in the absence of blood flow and cell binding). Clearance data are particularly important for in vitro work as they can be used in combination with in vivo data to predict the half-life and oral bioavailability of a drug.

[0373] The metabolic stability in hepatocytes assay involves a time-dependent reaction using both positive and negative controls. The cells must be pre-incubated at 37 °C then spiked with test compound (and positive control); samples taken at pre-determined time intervals are analysed to monitor the change in concentration of the initial drug compound over 60 minutes. A buffer incubation reaction (with no hepatocytes present) acts as a negative control and two cocktail solutions, containing compounds with known high and low clearance values (verapamil / 7-hydroxycoumarin and propranolol / diltiazem), act as positive controls. 1. The assay is run with a cell concentration of 0.5 x 10 6< cells / mL in Leibovitz buffer. 2. All compounds and controls are run in duplicate. 3. Compound concentration is 10µM. 4. All compounds and controls are incubated with both cells and buffer to show turnover is due to hepatic metabolism. 5. All wells on the incubation plate will have 326.7µL of either cells or buffer added. 6. Prior to assay, cell and buffer-only incubation plates are preincubated for 10 mins at 37 °C. 7. The assay is initiated by adding compounds, 3.3µL of 1mM in 10%DMSO-90%Buffer; final DMSO concentration is 0.1%. 8. Samples are taken at regular timepoints (0, 5, 10, 20, 40, 60 min) until 60 mins. 9. Sample volume is 40µL and it is added to 160µL of crash solvent (acetonitrile with internal standard) and stored on ice. 10. At the end of the assay, the crash plates are centrifuged at 3500rpm for 20mins at 4 °C. 11. 80µL of clear supernatant is removed and mixed with 80µL of deionised water before being analysed by LC-MS / MS.

[0374] Raw LC-MS / MS data are exported to, and analysed in, Microsoft Excel for determination of intrinsic clearance. The percentage remaining of a compound is monitored using the peak area of the initial concentration as 100%. Intrinsic clearance and half-life values are calculated using a graph of the natural log of percentage remaining versus the time of reaction in minutes. Half-life (min) and intrinsic clearance (Cl int in µL min -1< 10 -6< cells) values are calculated using the gradient of the graph (the elimination rate constant, k) and Equations 1 and 2. t 1 2 = ln 2 k CI int = ln 2 t 1 2 × 350 0.175

[0375] A number of compounds of formula (I) which were tested in Biological Example 4 were tested in this assay, and the results are shown in Table 6 below. 4-Octyl itaconate was included as a comparator compound. Table 6 - Hepatocyte stabilityCompound Species Cl int (µL min -1< 10 -6< cells) T½ (min) 4-octyl itaconateHuman4014Mouse3514Example 1Human10913Mouse13810Example 3Human2436Mouse1589Example 5Human1591Mouse52274-octyl itaconateHuman4014Mouse3514Example 7Human8616Mouse2206Example 9Human4431MouseNT*NTExample 10Human11136Mouse2165Example 11Human13413Mouse2977Example 12Human2949Mouse19813Example 13Human8183Mouse4730Example 14Human4352Mouse2948Example 17Human1685Mouse6023Example 19Human11126Mouse11212Example 21Human6525Mouse25513Example 41Human5268Mouse3834Example 42Human12120Mouse6420Example 51Human13129Mouse12913Example 54Human6028Mouse1879Example 61Human3254Mouse3505Example 62Human16109Mouse7922*NT means not tested in this assay

[0376] These results reveal that compounds of the invention, at least those of Table 6, are expected to have acceptable or improved metabolic stabilities, as shown by their intrinsic clearance (Cl int ) and half-life (T 1 / 2 ) values in this assay. All the compounds of formula (I) shown in Table 6 were more stable, i.e., they exhibited lower intrinsic clearance (Cl int ) and longer half-life (T 1 / 2 ) values compared with 4-octyl itaconate in at least human or mouse species. Preferred compounds exhibited lower intrinsic clearance (Cl int ) and longer half-life (T 1 / 2 ) values compared with 4-octyl itaconate in both human and mouse species.Biological Example 6 - Metabolites of compounds of formula (I)

[0377]

[0378] Example 21 undergoes hydrolysis in vivo to form Intermediate 6, as evidenced by a mouse pharmacokinetic study - carried out according to the protocol outlined in Biological Example 4 - the data for which are shown in Table 7. Intermediate 6 was tested in the assay described in Biological Examples 1 and 3 and the results are presented in Table 8. Example 21 is also shown as is dimethyl fumarate and dimethyl itaconate which were used as comparator compounds. Table 7 - Example 21 and Intermediate 6 concentrations (ng / mL) following oral administration at 100mg / kg to the mouseTime (h) Compound 0.083 0.25 0.5 1 2 4 6 8 24 Example 2110930011566774200279001400048834024867<8Intermediate 618467197671276747132157719614132BQL**BQL means below quantifiable limit

[0379] As shown in Table 7, these results reveal that Intermediate 6 (which is a metabolite of Example 21) displayed improved systemic exposure, as shown by the plasma concentrations of the compound in this assay. Intermediate 6 exhibited higher systemic exposure compared with dimethyl itaconate when administered orally. Table 8: Biological assay data for Intermediate 6Compound IL-1β (IC 50 ) IL-6 (IC 50 ) EC 50 (µM) E max (%) dimethyl fumarate14.49.35.6102dimethyl itaconate>100100.021.4137Example 2114.6NT*8.9173Intermediate 656.723.720.4165

[0380] As shown in Table 8, Intermediate 6 (which is a metabolite of Example 21) exhibited improved cytokine-lowering potencies compared to dimethyl itaconate for IL-1β and IL-6, and a lower EC 50 and a higher E max compared with dimethyl itaconate. Intermediate 6 also displayed a higher E max compared to dimethyl fumarate.References

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[0382] Throughout the specification and the claims which follow, unless the context requires otherwise, the word 'comprise', and variations such as 'comprises' and 'comprising', will be understood to imply the inclusion of a stated integer, step, group of integers or group of steps but not to the exclusion of any other integer, step, group of integers or group of steps.

Claims

1. A compound of formula (I): wherein, RA is selected from the group consisting of C1-10 alkyl, C3-10 cycloalkyl and C5-10 spirocycloalkyl; wherein RA is not substituted or is substituted by one or more substituents selected from the group consisting of oxo, R1A, OR2A, NR2AR3A, SR2A, SOR9A, SO2R9A, SO2NR2AR3A, C(O)R2A and CONR2AR3A; R1A is selected from the group consisting of fluoro, methyl, cyano, SiR4AR5AR6A, C3-8 cycloalkyl and phenyl; wherein methyl, C3-8 cycloalkyl and phenyl are not substituted or are substituted by R7A and / or R8A; R4A, R5A and R6A are independently selected from the group consisting of C1-4 alkyl and phenyl; R7A and R8A are independently selected from the group consisting of oxo, C1-4 alkyl, C1-4 alkoxy, C1-4 haloalkyl, C1-4 haloalkoxy, hydroxy, CO2H, cyano, methanesulfonyl and halo; or, taken together, R7A and R8A form a C3-8 cycloalkyl or 4-7 membered heterocyclic ring; R2A and R3A are independently H, C1-8 alkyl, C3-8 cycloalkyl or phenyl; or, taken together, R2A and R3A form a 4-7 membered heterocyclic ring; R9A is C1-8 alkyl, C3-8 cycloalkyl or phenyl; and RB is C1-2 alkyl substituted by CO2H and is optionally further substituted by trifluoromethyl or methyl; RC and RD are independently selected from the group consisting of H, C1-2 alkyl, hydroxy, methoxy and fluoro; or a pharmaceutically acceptable salt and / or solvate thereof.

2. The compound or a pharmaceutically acceptable salt and / or solvate thereof according to claim 1 wherein RA is C3-8 cycloalkyl.

3. The compound or a pharmaceutically acceptable salt and / or solvate thereof according to claim 1 or claim 2 wherein RA is substituted by R1A.

4. The compound or a pharmaceutically acceptable salt and / or solvate thereof according to claim 3 wherein R1A is phenyl and said phenyl is substituted by R7A or R8A.

5. The compound or a pharmaceutically acceptable salt and / or solvate thereof according to claim 4 wherein R7A is C1-4 haloalkyl such as CF3 and R8A is absent.

6. The compound or a pharmaceutically acceptable salt and / or solvate thereof according to any one of claims 1 to 5 wherein RB is C1 alkyl substituted by CO2H.

7. The compound or a pharmaceutically acceptable salt and / or solvate thereof according to any one of claims 1 to 6, wherein RC and RD are H.

8. The compound or a pharmaceutically acceptable salt and / or solvate thereof according to claim 1 which is selected from the list consisting of: 2-((2-methylene-4-(octyloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-(octyloxy)-4-oxobutanoyl)oxy)propanoic acid; 3-((2-methylene-4-(octyloxy)-4-oxobutanoyl)oxy)propanoic acid; 3-((4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; 3-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; 3-((2-methylene-4-(neopentyloxy)-4-oxobutanoyl)oxy)propanoic acid; (S)-3-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)propanoic acid; 3-((4-(hexyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; 3-((2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoyl)oxy)propanoic acid; 3-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; (R)-3-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)propanoic acid; (S)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-(neopentyloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((4-((4-fluorobenzyl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(hexyloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(3-phenoxypropoxy)butanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(spiro[3.3]heptan-2-yloxy)butanoyl)oxy)acetic acid; 2-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)propanoic acid; (R)-2-((2-methylene-4-(octan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((4-((4,4-difluorocyclohexyl)methoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(3-ethoxypropoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(bicyclo[2.2.1]heptan-2-yloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-cyclobutoxy-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(cyclohexyloxy)-2-methylene-4-oxobutanoyl)oxy)-3,3,3-trifluoropropanoic acid; 2-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)-3,3,3-trifluoropropanoic acid; 3-((4-(cyclooctyloxy)-2-methylene-4-oxobutanoyl)oxy)-4,4,4-trifluorobutanoic acid; 2-(4-(cycloheptyloxy)-2-methylene-4-oxobutanoyloxy)acetic acid; 2-(2-methylene-4-(octan-3-yloxy)-4-oxobutanoyloxy)acetic acid; 2-(2-methylene-4-(octan-4-yloxy)-4-oxobutanoyloxy)acetic acid; 2-((4-(heptan-4-yloxy)-2-methylene-4-oxobutanoyl)oxy) acetic acid; 2-((4-((adamantan-2-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(1-cyclohexylethoxy)-2-methylene-4-oxobutanoyl)oxy) acetic acid; 2-((4-(1-cycloheptylethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-(2-methylene-4-oxo-4-(spiro[3.4]octan-2-yloxy)butanoyloxy)acetic acid; 2-(2-methylene-4-oxo-4-(spiro[3.5]nonan-2-yloxy)butanoyloxy)acetic acid; 2-(2-methylene-4-oxo-4-(spiro[3.5]nonan-7-yloxy)butanoyloxy)acetic acid; 2-((4-((2,2-dimethylcyclohexyl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(cyclooctyloxy)-3-methyl-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(2,2,4,4-tetramethylcyclobutoxy)butanoyl)oxy) acetic acid; (S)-2-(2-methylene-4-(octan-3-yloxy)-4-oxobutanoyloxy)acetic acid; 2-((4-(cyclooctyloxy)-3-methoxy-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-((-adamantan-1-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (R)-2-((4-(heptan-2-yloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-(nonan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-(nonan-5-yloxy)-4-oxobutanoyl)oxy)acetic acid; 2-((4-(1-(3,5-dichlorophenyl)ethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-((1-(3,5-dichlorophenyl)propan-2-yl)oxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (R)-2-(2-methylene-4-(octan-3-yloxy)-4-oxobutanoyloxy)acetic acid; 2-((2-methylene-4-oxo-4-(((1R,2S,4R)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)butanoyl)oxy)acetic acid; 2-((4-(1-cyclohexyl-2,2,2-trifluoroethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((4-(bicyclo[3.3.1]nonan-9-yloxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (S)-2-((2-methylene-4-oxo-4-((1,1,1-trifluorooctan-2-yl)oxy)butanoyl)oxy)acetic acid; (R)-2-((2-methylene-4-(nonan-2-yloxy)-4-oxobutanoyl)oxy)acetic acid; (R)-2-((4-(1-cyclohexylethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (R)-4,4,4-trifluoro-3-((2-methylene-4-(((R)-octan-2-yl)oxy)-4-oxobutanoyl)oxy)butanoic acid; 2-((4-(cyclooctyloxy)-3-hydroxy-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-(3-methylene-5-(4-methylheptan-4-yloxy)-5-oxopent-1-en-2-yloxy)acetic acid; 2-((4-(1-cyclohexylcyclobutoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-((2-methyloctan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-((2-methylheptan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(1-pentylcyclobutoxy)butanoyl)oxy) acetic acid; 2-((2-methylene-4-((2-methylspiro[3.5]nonan-2-yl)oxy)-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-(((exo)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)oxy)butanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-((2,2,6,6-tetramethylcyclohexyl)oxy) butanoyl)oxy)acetic acid; 2-((4-(1-(3,5-dichlorophenyl)ethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid (Isomer 1); 2-((4-(1-(3,5-dichlorophenyl)ethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid (Isomer 2); (R)-2-((2-methylene-4-oxo-4-(1-(4-(trifluoromethyl)phenyl)ethoxy)butanoyl) oxy) acetic acid); (S)-2-((2-methylene-4-oxo-4-(1-(4-(trifluoromethyl)phenyl)ethoxy)butanoyl) oxy) acetic acid); 2-((4-(1-cyclohexylcyclopropoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; (S)-2-((4-(1-cyclohexylethoxy)-2-methylene-4-oxobutanoyl)oxy)acetic acid; 2-((2-methylene-4-oxo-4-((2,2,4,4-tetramethylpentan-3-yl)oxy)butanoyl) oxy)acetic acid; (S)-4,4,4-trifluoro-3-((2-methylene-4-(((R)-octan-2-yl)oxy)-4-oxobutanoyl)oxy)butanoic acid; 2-((2-methylene-4-oxo-4-(1-pentylcyclopropoxy)butanoyl)oxy) acetic acid; and 3-((2-methylene-4-oxo-4-(2,2,4,4-tetramethylcyclobutoxy)butanoyl)oxy)propanoic acid; or a pharmaceutically acceptable salt and / or solvate of any one thereof.

9. A pharmaceutical composition comprising a compound or a pharmaceutically acceptable salt and / or solvate thereof according to any one of claims 1 to 8.

10. A compound or a pharmaceutically acceptable salt and / or solvate thereof according to any one of claims 1 to 8 or a pharmaceutical composition according to claim 9 for use as a medicament.

11. A compound or a pharmaceutically acceptable salt and / or solvate thereof according to any one of claims 1 to 8 or a pharmaceutical composition according to claim 9 for use in treating or preventing an inflammatory disease or a disease associated with an undesirable immune response.

12. The compound for use according to claim 11, wherein the inflammatory disease or disease associated with an undesirable immune response is, or is associated with, a disease selected from the group consisting of: psoriasis (including chronic plaque, erythrodermic, pustular, guttate, inverse and nail variants), asthma, chronic obstructive pulmonary disease (COPD, including chronic bronchitis and emphysema), heart failure (including left ventricular failure), myocardial infarction, angina pectoris, other atherosclerosis and / or atherothrombosis-related disorders (including peripheral vascular disease and ischaemic stroke), a mitochondrial and neurodegenerative disease (such as Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, retinitis pigmentosa or mitochondrial encephalomyopathy), autoimmune paraneoplastic retinopathy, transplantation rejection (including antibody-mediated and T cell-mediated forms), multiple sclerosis, transverse myelitis, ischaemia-reperfusion injury (e.g. during elective surgery such as cardiopulmonary bypass for coronary artery bypass grafting or other cardiac surgery, following percutaneous coronary intervention, following treatment of acute ST-elevation myocardial infarction or ischaemic stroke, organ transplantation, or acute compartment syndrome), AGE-induced genome damage, an inflammatory bowel disease (e.g. Crohn's disease or ulcerative colitis), primary sclerosing cholangitis (PSC), PSC-autoimmune hepatitis overlap syndrome, non-alcoholic fatty liver disease (non-alcoholic steatohepatitis), rheumatica, granuloma annulare, cutaneous lupus erythematosus (CLE), systemic lupus erythematosus (SLE), lupus nephritis, drug-induced lupus, autoimmune myocarditis or myopericarditis, Dressler's syndrome, giant cell myocarditis, post-pericardiotomy syndrome, drug-induced hypersensitivity syndromes (including hypersensitivity myocarditis), eczema, sarcoidosis, erythema nodosum, acute disseminated encephalomyelitis (ADEM), neuromyelitis optica spectrum disorders, MOG (myelin oligodendrocyte glycoprotein) antibody-associated disorders (including MOG-EM), optic neuritis, CLIPPERS (chronic lymphocytic inflammation with pontine perivascular enhancement responsive to steroids), diffuse myelinoclastic sclerosis, Addison's disease, alopecia areata, ankylosing spondylitis, other spondyloarthritides (including peripheral spondyloarthritis, that is associated with psoriasis, inflammatory bowel disease, reactive arthritis or juvenile onset forms), antiphospholipid antibody syndrome, autoimmune hemolytic anaemia, autoimmune hepatitis, autoimmune inner ear disease, pemphigoid (including bullous pemphigoid, mucous membrane pemphigoid, cicatricial pemphigoid, herpes gestationis or pemphigoid gestationis, ocular cicatricial pemphigoid), linear IgA disease, Behçet's disease, celiac disease, Chagas disease, dermatomyositis, diabetes mellitus type I, endometriosis, Goodpasture's syndrome, Graves' disease, Guillain-Barre syndrome and its subtypes (including acute inflammatory demyelinating polyneuropathy, AIDP, acute motor axonal neuropathy (AMAN), acute motor and sensory axonal neuropathy (AMSAN), pharyngeal-cervical-brachial variant, Miller-Fisher variant and Bickerstaff's brainstem encephalitis), progressive inflammatory neuropathy, Hashimoto's disease, hidradenitis suppurativa, inclusion body myositis, necrotising myopathy, Kawasaki disease, IgA nephropathy, Henoch-Schonlein purpura, idiopathic thrombocytopenic purpura, thrombotic thrombocytopenic purpura (TTP), Evans' syndrome, interstitial cystitis, mixed connective tissue disease, undifferentiated connective tissue disease, morphea, myasthenia gravis (including MuSK antibody positive and seronegative variants), narcolepsy, neuromyotonia, pemphigus vulgaris, pernicious anaemia, psoriatic arthritis, polymyositis, primary biliary cholangitis (also known as primary biliary cirrhosis), rheumatoid arthritis, palindromic rheumatism, schizophrenia, autoimmune (meningo-)encephalitis syndromes, scleroderma, Sjogren's syndrome, stiff person syndrome, polymylagia rheumatica, giant cell arteritis (temporal arteritis), Takayasu arteritis, polyarteritis nodosa, Kawasaki disease, granulomatosis with polyangitis (GPA; formerly known as Wegener's granulomatosis), eosinophilic granulomatosis with polyangiitis (EGPA; formerly known as Churg-Strauss syndrome), microscopic polyarteritis / polyangiitis, hypocomplementaemic urticarial vasculitis, hypersensitivity vasculitis, cryoglobulinemia, thromboangiitis obliterans (Buerger's disease), vasculitis, leukocytoclastic vasculitis, vitiligo, acute disseminated encephalomyelitis, adrenoleukodystrophy, Alexander's disease, Alper's disease, balo concentric sclerosis or Marburg disease, cryptogenic organising pneumonia (formerly known as bronchiolitis obliterans organizing pneumonia), Canavan disease, central nervous system vasculitic syndrome, Charcot-Marie-Tooth disease, childhood ataxia with central nervous system hypomyelination, chronic inflammatory demyelinating polyneuropathy (CIDP), diabetic retinopathy, globoid cell leukodystrophy (Krabbe disease), graft-versus-host disease (GVHD) (including acute and chronic forms, as well as intestinal GVHD), hepatitis C (HCV) infection or complication, herpes simplex viral infection or complication, human immunodeficiency virus (HIV) infection or complication, lichen planus, monomelic amyotrophy, cystic fibrosis, pulmonary arterial hypertension (PAH, including idiopathic PAH), lung sarcoidosis, idiopathic pulmonary fibrosis, paediatric asthma, atopic dermatitis, allergic dermatitis, contact dermatitis, allergic rhinitis, rhinitis, sinusitis, conjunctivitis, allergic conjunctivitis, keratoconjunctivitis sicca, dry eye, xerophthalmia, glaucoma, macular oedema, diabetic macular oedema, central retinal vein occlusion (CRVO), macular degeneration (including dry and / or wet age related macular degeneration, AMD), post-operative cataract inflammation, uveitis (including posterior, anterior, intermediate and pan uveitis), iridocyclitis, scleritis, corneal graft and limbal cell transplant rejection, gluten sensitive enteropathy (coeliac disease), dermatitis herpetiformis, eosinophilic esophagitis, achalasia, autoimmune dysautonomia, autoimmune encephalomyelitis, autoimmune oophoritis, autoimmune orchitis, autoimmune pancreatitis, aortitis and periaortitis, autoimmune retinopathy, autoimmune urticaria, Behcet's disease, (idiopathic) Castleman's disease, Cogan's syndrome, IgG4-related disease, retroperitoneal fibrosis, juvenile idiopathic arthritis including systemic juvenile idiopathic arthritis (Still's disease), adult-onset Still's disease, ligneous conjunctivitis, Mooren's ulcer, pityriasis lichenoides et varioliformis acuta (PLEVA, also known as Mucha-Habermann disease), multifocal motor neuropathy (MMN), paediatric acute-onset neuropsychiatric syndrome (PANS) (including paediatric autoimmune neuropsychiatric disorders associated with streptococcal infections (PANDAS)), paraneoplastic syndromes (including paraneoplastic cerebellar degeneration, Lambert-Eaton myaesthenic syndrome, limbic encephalitis, brainstem encephalitis, opsoclonus myoclonus ataxia syndrome, anti-NMDA receptor encephalitis, thymoma-associated multiorgan autoimmunity), perivenous encephalomyelitis, reflex sympathetic dystrophy, relapsing polychondritis, sperm & testicular autoimmunity, Susac's syndrome, Tolosa-Hunt syndrome, Vogt-Koyanagi-Harada Disease, anti-synthetase syndrome, autoimmune enteropathy, immune dysregulation polyendocrinopathy enteropathy X-linked (IPEX), microscopic colitis, autoimmune lymphoproliferative syndrome (ALPS), autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy syndrome (APEX), gout, pseudogout, amyloid (including AA or secondary amyloidosis), eosinophilic fasciitis (Shulman syndrome) progesterone hypersensitivity (including progesterone dermatitis), amilial Mediterranean fever (FMF), tumour necrosis factor (TNF) receptor-associated periodic fever syndrome (TRAPS), hyperimmunoglobulinaemia D with periodic fever syndrome (HIDS), PAPA (pyogenic arthritis, pyoderma gangrenosum, severe cystic acne) syndrome, deficiency of interleukin-1 receptor antagonist (DIRA), deficiency of the interleukin-36-receptor antagonist (DITRA), cryopyrin-associated periodic syndromes (CAPS) (including familial cold autoinflammatory syndrome [FCAS], Muckle-Wells syndrome, neonatal onset multisystem inflammatory disease [NOMID]), NLRP12-associated autoinflammatory disorders (NLRP12AD), periodic fever aphthous stomatitis (PFAPA), chronic atypical neutrophilic dermatosis with lipodystrophy and elevated temperature (CANDLE), Majeed syndrome, Blau syndrome (also known as juvenile systemic granulomatosis), macrophage activation syndrome, chronic recurrent multifocal osteomyelitis (CRMO), familial cold autoinflammatory syndrome, mutant adenosine deaminase 2 and monogenic interferonopathies (including Aicardi-Goutières syndrome, retinal vasculopathy with cerebral leukodystrophy, spondyloenchondrodysplasia, STING [stimulator of interferon genes]-associated vasculopathy with onset in infancy, proteasome associated autoinflammatory syndromes, familial chilblain lupus, dyschromatosis symmetrica hereditaria), Schnitzler syndrome; familial cylindromatosis, congenital B cell lymphocytosis, OTULIN-related autoinflammatory syndrome, type 2 diabetes mellitus, insulin resistance and the metabolic syndrome (including obesity-associated inflammation), atherosclerotic disorders (e.g. myocardial infarction, angina, ischaemic heart failure, ischaemic nephropathy, ischaemic stroke, peripheral vascular disease, aortic aneurysm), renal inflammatory disorders (e.g. diabetic nephropathy, membranous nephropathy, minimal change disease, crescentic glomerulonephritis, acute kidney injury, renal transplantation).

13. A compound which is a compound of formula (IX): wherein: RB' is RB or a derivative thereof in which the carboxylic acid of RB is protected by an alkyl, benzyl or silyl group; wherein RB is as defined in any one of claims 1 to 8; RC and RD are as defined in any one of claims 1 to 8; or a salt thereof.

14. A process for preparing a compound or a pharmaceutically acceptable salt and / or solvate thereof according to any one of claims 1 to 8 which comprises: (a) reacting a compound of formula (III): or a salt thereof; with a compound of formula (II):         RB'-OH     (II) or a salt thereof; (b) reacting a compound of formula (III): or a salt thereof; with a compound of formula (II'):         RB'-X     (II') or a salt thereof; (c) reacting a compound of formula (IX): or a salt thereof; with a compound of formula (IV)         RA'-OH     (IV) or a salt thereof; or (d) reacting a compound of formula (XIII): or a salt thereof, with formaldehyde or an equivalent thereof; wherein: RA' is RA or a protected derivative thereof; wherein RA is as defined in any one of claims 1 to 8; RB' is RB or a protected derivative thereof; wherein RB is as defined in any one of claims 1 to 8; RC and RD are as defined in any one of claims 1 to 8; X is a leaving group; R11 is C1-4 alkyl optionally substituted with halo; and R12 is C1-4 alkyl optionally substituted with halo.