Cell tissue culture and transport canister

Abstract

The invention relates to a cell tissue culture and transport canister comprising: a canister body having an accessible interior and an upper opening; a filter arranged inside the canister body or outside the canister and placing the interior of the canister body in communication with an exterior of the canister body, the filter being configured to allow a gas exchange between the interior and the exterior of the canister body, while preventing contamination of the cell tissue culture; a closure means closing the upper opening of the canister body, the closure means being sealed or slightly permeable with respect to fluids and gases; and at least one aseptic fluid connection through the canister body, the at least one aseptic fluid connection including two fluid inlet connections and one fluid outlet connection.

Description

Description Title: Cell tissue culture and transport dish Technical field

[0001] This disclosure relates to the field of cell tissue culture and transport dishes. Prior art

[0002] The process of manufacturing cellular tissue, such as artificial skin, involves the use of a sterile environment and a variety of culture media that are added to the culture sequentially. The process can be time-consuming and involve extensive manipulation of the closed system.

[0003] Typically, stem cells are placed in a culture dish. This culture dish constitutes a closed environment in which the different layers of the skin will be created through the addition of culture media. Each time a medium needs to be added to the culture, the operator uncaps / unscrews / opens the dish and introduces the culture medium. Then he recaps / rescrews / closes the dish, replaces it and lets everything settle. He takes the dish, uncaps, introduces, and recaps it in this way for each culture medium.

[0004] On the one hand, the multiple operations of opening and closing the box can lead to contamination of the culture medium. On the other hand, this requires a large amount of operator time, which does not allow for large-scale multiplication of cell tissue culture. Summary

[0005] There is provided a cell tissue culture and transport dish comprising: a dish body having an accessible interior and an upper opening allowing communication of the interior with an exterior of the dish body; a filter disposed in the dish body or outside the dish body, the filter communicating the interior with the exterior of the dish body, the filter being configured to allow gas exchange between the interior and the exterior of the dish body, while preventing contamination of the cell tissue culture; a closure means closing the upper opening of the dish body, the closure means being tight or weakly permeable to fluids and gases; and at least one aseptic fluid connection through the dish body, said at least one aseptic fluid connection including two fluid inlet fluid connections and one fluid outlet fluid connection. The features set out in the following paragraphs may, optionally, be implemented, independently of each other or in combination with each other: - the closing means is single-use so that once detached from the upper opening, the inside of the box body is accessible through the upper opening - the closing means is a lid or a cover - the closing means is sealed, screwed or clipped to the box body, for example by an O-ring, heat sealing or ultrasound - the closing means is transparent - the closing means retains its mechanical properties up to an exposure temperature of approximately -200°C. - the closure means is made of polyethylene terephthalate or polyethylene. - the filter is arranged at the level of said at least one fluid connection. - the filter is higher than the culture medium to ensure its gas exchange function and not to be wetted by the culture medium. - the filter is arranged in a horizontal part or in an inclined part of an interior of the box body - the inclined part being inclined towards the insert. - the filter is arranged vertically below a gripping area of ​​the insert. - the filter is placed in the distal box body of a culture zone of the insert. - the filter is made of polyethylene, polyurethane, polyester or polypropylene. - the filter is hydrophobic, preferably on both sides. - the box further comprises a removable insert adapted to be placed inside the box body, the insert being adapted to receive a cell tissue culture. - the insert is removable from the box body through the upper opening. - the insert has a growing area and a gripping area connected to the growing area, the gripping area being adapted to manipulate the insert relative to the box body. - the culture area includes a top opening suitable for accommodating cell tissue culture. - the growing area includes a porous bottom. -the porous bottom is opposite the upper opening when the insert is placed in the box body. - the porous bottom of the insert is made of polyester, polyethylene or polycarbonate - the pore size of the porous bottom is 0.2 to 4pm, preferably 0.2 to 0.6pm / - the pore density of the porous bottom is 1*10 6 at 5*10 6 , preferably 1 * 10 6 at 3*10 6 pores per cm 2 . - the gripping area is inclined towards the growing area - the growing area of ​​the insert is rounded in shape - the growing area of ​​the insert is a ring - the growing area of ​​the insert has a thickness corresponding to a thickness of a working surface of the box body adapted to receive the growing area of ​​the insert. - the growing area of ​​the insert has a shape corresponding to a shape of a working surface of the box body adapted to receive the growing area of ​​the insert. - said insert is resistant to dimethylsulfoxide (DMSO). - the insert includes at least one connection adapted to cooperate removably with the interior of the box body. - said at least one connection is at least one tab adapted to be removably inserted into a housing inside the box body. - the housing is an open recess. - the insert is made of polyester, polypropylene, fluorinated ethylene propylene, polycarbonate. - the interior of the box body includes a working area suitable for receiving the cell tissue culture and fluid culture media allowing the cell tissue to grow. - the work area includes a work surface suitable for receiving the growing area of ​​the insert - the work surface being preferably flat. - the work surface has undergone plasma surface treatment or corona treatment. - the inside of the box body includes a portion inclined towards the work surface. - the work surface is transparent. - the box body is substantially parallelepipedal and has rounded edges. - the work surface is removable from the rest of the box body. - the box body is made of polyester, polypropylene, fluorinated ethylene propylene, polycarbonate, polycarbonate alloy or Eastman Tritan™ Copolyester MP100. - the two fluid inlet connections are arranged vertically above the culture area. - the fluid outlet fluid connection is arranged vertically at the same level or below the culture area. - the two fluid inlet fluid connections are ports having a tubular extension extending towards the inside of the box body. - the tubular extension has one end located vertically above the insert. - one end of the inlet fluid connections into the inside of the box body is beveled. - the bevel is oriented towards the upper opening. - the bevel is approximately 45 degrees. - the tubular extension is sealed to the box body by welding and the bevels obtained using an aseptic disconnection machine. - the fluid outlet fluid connection is a septum, a clamp, a fitting grooved associated with a radial crimping system or a Luer-Lock type conical fitting. - said at least one fluid connection is resistant to dimethylsulfoxide (DMSO). - the box body also includes a partition separating the working area from the filter. Brief description of the drawings

[0006] Other features, details and advantages will become apparent upon reading the detailed description below, and upon analyzing the attached drawings, in which: Fig. 1

[0007] [Fig. 1] is a schematic perspective view from above of a cell tissue culture and transport dish according to one embodiment; Fig. 2

[0008] [Fig. 2] is a schematic perspective view of the underside of the box of Fig. 1; Fig. 3

[0009] [Fig. 3] is another schematic perspective view from above of the box of Fig. 1; Fig. 4

[0010] [Fig. 4] is a schematic sectional view of the box of Fig. 1;

[0011] ; Fig. 5

[0012] [Fig. 5] is a schematic perspective view of an insert for the box of Fig. 1; and Fig. 6

[0013] [Fig. 6] is a schematic view of one embodiment of the insert where a porous bottom of the insert is detachable; Fig. 7

[0014] [Fig. 7] is a schematic view of another embodiment of the insert where a porous bottom of the insert is detachable; Fig. 8

[0015] [Fig. 8] is a schematic view of one embodiment of the box where the box has a removable bottom; and Fig. 9

[0016] [Fig. 9] is a schematic view of another embodiment of the box where a filter is arranged vertically. Fig. 10

[0017] [Fig. 10] is a schematic view of another embodiment of the box which includes a partition between the filter and the working area. Description of the embodiments

[0018] With reference to Figures 1 to 4, a box 10 for culturing and transporting cellular tissue TC according to one embodiment will be described. The box 10 comprises a box body 12 having an accessible interior 14 and an upper opening 16. The upper opening 16 is closed by a closure means 18. The closure means 18 is preferably fluid and gas tight. According to another embodiment, the filter is slightly permeable to fluids and gases. A filter is slightly permeable if the water vapor transmission rate is less than 5g / m 2 / 24h, preferably 1 g / m 2 / 24h.

[0019] The box 10 comprises at least one aseptic fluid connection 20 through the box body 12. In the example of FIG. 1, the box 10 includes three aseptic fluid connections 20: two inlet fluid connections 22 for culture fluids, and one outlet fluid connection 24 for fluids. The inlet fluid connections 22 allow fluids, preferably liquids, to be conveyed for the culture of TC cell tissue, while the outlet fluid connection 24 allows the TC cell tissue culture to be drained of these same fluids. The interior 14 of the box body 12 includes a working area (or volume) 36 adapted to receive the TC cell tissue culture and the fluid culture media allowing the TC cell tissue to grow.

[0020] The box 10 further includes a filter 26 disposed in the box body 12 (i.e., in the wall delimiting the interior from the exterior of the box 10). The filter 26 communicates the interior 14 of the box body 12 with an exterior 28 of the box body 12. The filter 26 is configured to allow gas exchange between the interior 14 and the exterior 28 of the box body 21, while preventing the passage of liquids. The filter 26 is configured to prevent the passage of bacteria and viruses. The filter 26 is the only means of communication between the interior and the exterior of the box 10, apart from the three aseptic fluid connections 20.

[0021] According to one embodiment, the filter 26 is not arranged in the box body 12 but outside 28 of the box body 12. It would communicate with the interior 14 of the box body 12 by means of a connecting means, such as a tube connected to the box body 12. Positioning the filter outside 28 of the box body 12 makes it possible to avoid pressure deltas (of the order of -0.5 bar) during cryopreservation.

[0022] According to one embodiment, the filter is hydrophobic. According to one embodiment, a hydrophobic treatment has been applied to each face of the filter.

[0023] The box 10 may optionally include a removable insert 30 disposed in the working area 36 and adapted to receive the TC cell tissue culture. The insert 30 can be inserted into the interior 14 of the dish body 12 through the upper opening 16. Therefore, the upper opening 16 is sized to allow the passage of the insert 30. The insert 30 can have a culture area 34 adapted to receive and culture the TC cell tissue and a gripping area 35 allowing the insert 30 to be manipulated, in particular to put it on and / or remove it from the dish body 12. The insert 30 will be described in more detail below, in relation to FIG. 5. According to other embodiments, the dish 10 could not have an insert, so that the TC cell tissue culture is directly carried out on the interior 14 of the dish body 12 at the working area 36.

[0024] The working area 36 is sized to contain an adequate volume of culture liquid. In the case of the insert 30, the working area 36 has a volume slightly larger than that of the culture area 34 of the insert 30.

[0025] The working area 36 of the dish body 12 includes a working surface 32 adapted to receive the TC cell tissue culture. In the case of dishes having an insert, the working surface 32 is adapted to receive the culture area 34 of the insert 30. In the case of dishes 10 without an insert, the TC cell tissue will be directly arranged on the working surface 32 of the dish 10. In the latter case, the working surface 32 may have undergone a plasma surface treatment in order to allow the TC cell tissue to adhere to the working surface 32. The working surface 32 could alternatively have undergone a corona treatment.

[0026] Whether with or without an insert, the working surface 32 may for example be transparent in order to allow visual inspection of the cellular tissue culture TC, without having to open the closing means 18. The working surface 32 may be part of the box body 12 delimiting the interior 14 from the exterior 28. More broadly, all or part of the box body 12 could be transparent. The working surface 32 is preferably flat. It may be a reinforcement of the box body 12, as illustrated in the figures. The working surface 32 may be detachable from the box body 12 so as to extract the TC cellular tissue when it is formed, as will be explained in relation to Figures 6 to 8.

[0027] The closure means 8 serves to provide a closed system in the dish 10 that allows the culture of TC cell tissue. The closure means 18 may be sealed to the dish body 12, for example by heat sealing or ultrasound. The closure means 18 may include a tab 19 that allows the closure means 8 to be removed by hand without the use of instruments, such as a scalpel.

[0028] The closure means 18 may be disposable so that once detached from the upper opening, 16 the interior 14 of the box body 12 is accessible through the upper opening 16. Thus, the box 10 for culturing and transporting cell tissue may be a disposable product. In the example of the figures, the closure means 18 is transparent in order to allow visual inspection of the cell tissue culture. It may be that the closure means 18 is not transparent, or partially transparent, colored or clear. In order to withstand cryogenics during transport and storage of the cell tissue culture, the closure means 18 is preferably adapted to retain its mechanical properties up to an exposure temperature of approximately -200°C. The closure means 18 is for example made of polyethylene terephthalate or polyethylene.

[0029] The box body 12 may be made by plastic injection. The box body 12 may be made, for example, of polypropylene, fluorinated ethylene propylene, polycarbonate, or Eastman Tritan™ Copolyester MP100. The box body 12 may be adapted to retain its mechanical properties down to an exposure temperature of approximately -200°C, in order to allow cryogenics of the box 10. The box body 12 may be resistant to dimethyl sulfoxide (DMSO). The box body 12 may be substantially parallelepipedal with rounded edges in order to facilitate transport without loss of space. The box body 12 may have a generally ovoid shape when viewed from above.

[0030] The box body 12 can thus include a reinforcement 13 making it possible to receive one end of the gripping zone 35 of the insert 30 so that the insert 30 does not protrude from the box 10.

[0031] The interior 14 of the box body 12 may include an inclined portion 38 toward the working surface 32. The inclined portion 38 may allow the flow of fluids toward the TC cell tissue culture area 36. The inclination of the inclined portion 38 is with reference to a horizontal plane H, the horizontal plane H containing the working surface 32. The inclined portion 38 is, according to one embodiment, opposite the fluid connections 20.

[0032] The inclined portion 38 may receive the filter 26. The filter 26 may be arranged horizontally in the inclined portion 38 or alternatively be inclined, for example having the same inclination as that of the inclined portion as illustrated in Figures 1 to 4. The filter 26 could be arranged vertically in the inclined portion 38 as illustrated by the filter 26' of Figure 9. An evaluation channel C could then extend from the inclined portion 38 through the filter 26' and out of the box. According to another embodiment, the filter 26 is arranged at the level of the fluid connections 20. The filter 26 can be arranged in the box body 12 distally of the working surface 32. This makes it possible to limit the risks of humidification of the filter 26. According to one embodiment, and as shown in the figures, the filter 26 can be arranged vertically below the gripping zone 35 of the insert 30.The filter 26 may be made of polyethylene, polyurethane, polyester or polypropylene. In order to withstand cryogenics during transport and storage of the TC cell tissue culture, the filter 26 is preferably adapted to retain its mechanical properties down to an exposure temperature of approximately -200°C. The box 10 could include more than one filter.

[0033] According to another embodiment, the filter 26 is arranged in the box body 12 at the level of the fluid connections 20. According to one embodiment, the filter 26 is arranged on the box body 12 opposite the fluid connections 20. This latter configuration can make it possible to limit the risks of humidification of the filter 26.

[0034] The two fluid inlet connections 22 are ports having an interior tubular extension 40 extending toward the interior 14 of the box body 12. Each tubular extension 40 has one end that is vertically above the working surface 32, or if the box has an insert 30, vertically above the culture area 34 of the insert 30. The interior tubular extensions 40 are sized to be long enough to supply the culture medium, but at the same time not so long as to interfere with the removal of the insert 30. According to one embodiment, one end 42 of the inlet fluid connections 22 in the interior 14 of the box body 12 is beveled. The beveled shape allows flow towards the growing area 34 / working surface 32. The bevel is for example oriented towards the upper opening 16. The bevel may be approximately 45 degrees.The inner tubular extensions 40 may be sealed to the box body 12 by welding and the bevels obtained using an aseptic disconnecting machine.

[0035] The fluid connections 20 also each have an outer tubular extension 41 extending from the outside of the box body 12 so as to connect to tubes T containing culture or drainage liquids (shown in dotted lines). The outer tubular extensions 41 are adapted to connect to the tubes T for example by a male-female system with a serflex which ensures the seal, a mechanical system, by ultrasonic welding or using biocompatible glue.

[0036] The fluid inlet connections 22 and / or the fluid outlet connection 24 may be a septum allowing them to be pierced by a needle while ensuring a seal. The fluid inlet connections 22 and / or the fluid outlet connection 24 may be resistant to dimethylsulfoxide (DMSO) or any other preservation medium injected into the dish at the end of the cell culture process.

[0037] The dish 10 could also have external attachments, for example a slot in the exterior of the dish body 12, to allow the dish to be temporarily attached to a support allowing the dish to be supplied with culture media. The support can thus contain a plurality of dishes 10 to ensure parallel production of the TC cell tissue.

[0038] As mentioned previously, the removable insert 30 comprises the culture zone 34 adapted to receive and cultivate the cellular tissue TC and the gripping zone 35 allowing the insert 30 to be manipulated, in particular to put it on and / or remove it from the box body 12. This manipulation can be done using the fingertips, or if the box 10 allows it (in particular due to the size of the cavity located under the gripping zone 35 and visible in FIG. 4 by the inclined part 38) with one end of the phalanges.

[0039] The insert 30 (the growing area 34 and gripping area 35) may be made of polyester, polypropylene, fluorinated ethylene propylene, polycarbonate.

[0040] The culture area 34 may include an upper opening 44 adapted to receive the TC cell tissue culture in order to feed the cells from above, in particular through the inlet fluid connections 22. The culture area 34 includes a porous bottom 46 opposite the upper opening 16 when the insert 30 is disposed in the dish body 12 on the working surface 32. The porous bottom 46 of the insert 30 allows the TC cell tissue culture to be received. It is porous in order to allow the fluid culture medium to soak the top and bottom of the TC cell tissue culture. The porous bottom 46 may be made of polyethylene or polycarbonate. The porous bottom 46 may be transparent. The porous bottom 46 could be flexible or rigid. It could have undergone a plasma treatment. Porous means that the bottom allows the evacuation of liquids either through a network of open cells or one or more evacuation holes.The porous bottom 46 could be detachable or not from the rest of the insert 30. According to one embodiment, the size of the pores of the porous bottom is from 0.2 to 4 pm, preferably from 0.2 to 0.6 pm. According to one embodiment, the density of the pores of the porous bottom is 1 * 10. 6 at 5*10 6 , preferably 1 * 10 6 at 3*10 6 pores per cm 2 .

[0041] The culture zone 34 of the insert 30 is generally rounded in shape, for example ring-shaped. The culture zone 34 of the insert 30 may have a thickness E that is less than or corresponds to a thickness of the working surface 38 of the box body 12 adapted to receive the culture zone 34 of the insert 30. This may allow good flow of fluids. According to one embodiment, the culture zone 34 of the insert 30 has a shape corresponding to a shape of the working surface 32 of the box body 12 adapted to receive the culture zone 34 of the insert 30 to prevent the insert 30 from moving relative to the box body 12 during transport.

[0042] The gripping area 34 is, according to one embodiment and as illustrated in the figures, a handle. The gripping area 34 could, however, have any shape allowing the insert 30 to be manipulated. In the embodiment illustrated in the figures, the gripping area 34 is arranged opposite the inclined portion 38. The gripping area 34 may have an inclination relative to the horizontal plane H in the direction of the culture surface 32 in order to allow the flow of condensed fluids, for example. The inclination of the gripping area 34 relative to the horizontal plane H may be substantially the inclination of the inclined portion 38.

[0043] The insert 30 may further include one (or more) connections 47 arranged on the ring-shaped growing area 34, and adapted to removably cooperate with the interior 14 of the box body 12. The connections 47 may prevent the insert 30 from moving relative to the box body 12 during transport. According to one embodiment, and as illustrated in the figures, the connections 47 are tabs adapted to be removably inserted into a housing 48 of the interior 14 of the box body 12. The housing 48 may be an open recess in order to remove the insert 30 upwards.

[0044] The insert 30 may not have the connections 47. On the other hand, the insert 30 may have tabs 49 (2 or more) arranged on the lateral edges of the insert 30 with, for example, hollows at the level of the tabs for placing and sliding the insert 30. The tabs 49 are illustrated in Figure 4 in dotted lines. The tabs 49 make it possible to reduce the quantity of culture medium required for cell culture.

[0045] The insert 30 may be made of polypropylene, fluorinated ethylene propylene, or polycarbonate. The insert 30 may be resistant to dimethyl sulfoxide (DMSO).

[0046] The box 10 is usable as follows. Initially, the box 10 is delivered with the sealed closure means 18, and the insert 30 inside 14 of the box 10, if the box 10 has such an insert. The insert 30 may contain cells in the working area 36 (i.e., on the working surface 32 if the box does not have an insert, or on the porous bottom 46 if the box has an insert) which will be used to culture the cellular tissue TC. The box 10 is then connected via the fluid connections 20 to culture media by the tubes T. There may be one or more culture media that are conveyed into the work area 36 by the inlet fluid connections 22, sequentially, with (or not) rest periods between. The outlet fluid connection 24 allows the work area 36 to be drained between the different conveyances of culture media. The culture of the TC cell tissue is therefore carried out in a closed environment with little risk of contamination. When the TC cell tissue is created, the box 10 can be cryogenically frozen and transported. When the operator decides to use the TC cell tissue, he can recover it in several ways. He can open the closure means 18 and recover the TC cell tissue. If the box 10 has the insert, he can then remove the insert through the upper opening 16 thus released from the closure means 18.

[0047] According to one embodiment, and as illustrated in FIG. 6, the porous bottom 46 of the insert 30 can be detachable, if necessary via a tab, so as to extract the cellular tissue TC from the insert 30 without damaging it. Firstly, the porous bottom 46 with the cellular tissue TC thereon can be detached from the insert 30, then the porous bottom 46 removed from the cellular tissue TC so as to be able to apply the cellular tissue TC alone, for example, to the skin of the patient S.

[0048] According to another embodiment and as illustrated in Figure 7, the porous base 46 is dissociated from the insert 30 and the cellular tissue TC remains attached to the insert 30 for a time. The insert 30 is then applied to the skin S of the patient in order to place the cellular tissue TC on the skin S of the patient without directly manipulating it.

[0049] On the other hand, the box 10 could have a removable bottom for recovering the TC cell tissue, especially when the box 10 does not have an insert. In relation to Figure 8, the working surface 32 of the box body 12 is removable, and is preferably flexible. When the TC cell tissue is formed and is ready for use, the working surface 32 is then removed from the box body 12 and with it the TC cell tissue. The TC cell tissue is then removed from the working surface 32 and deposited on the patient's skin S.

[0050] Referring now to Figure 10, another embodiment of the box 10, which we will call box 110, will be described. The box 110 includes all the elements and embodiments of the box 10, which will not be described again and for which similar numerical references but in the hundreds will be used.

[0051] The box 110 is distinguished from the box 10 by the fact that the box body 112 comprises a partition 150 separating the working area 136 from the filter 126. More specifically, in the embodiment of the figures, the partition 150 is located between the working area 136 and the inclined portion 138 of the interior 114 of the box body 112. As such, and as it is illustrated in Figure 10, in this embodiment, the part 138 may not be inclined. It is also positioned in a lower position than the part 38 so as to prevent the partition 150 from touching the closing means 118 closing the upper opening 116. Generally, the partition 150 is vertical and does not touch the closing means 118 closing the upper opening 116 of the box body 112. The partition 150 makes it possible to secure the filter and prevent it from getting wet when handling the box and during the stirring phases.

Claims

Claims

1. A box (10, 110) for culturing and transporting cell tissue (TC) comprising: - a box body (12) having an accessible interior (14) and an upper opening (16) allowing communication of the interior (14) with an exterior (28) of the box body (12); - a filter (26) disposed in the box body (12) or outside (28) of the box body (12), the filter (26) communicating the interior (14) with the exterior (28) of the box body (12), the filter (26) being configured to allow gas exchange between the interior (14) and the exterior (28) of the box body (12), while preventing contamination of the cell tissue culture; - a closing means (18) closing the upper opening (16) of the box body (12), the closing means (18) being tight or weakly permeable to fluids and gases; and - at least one aseptic fluid connection (20) through the box body (12), said at least one aseptic fluid connection (20) including two fluid inlet fluid connections (22) and one fluid outlet fluid connection (24).

2. A box (10, 110) according to claim 1, wherein the closure means (18) is disposable so that once detached from the upper opening (16), the interior (14) of the box body (12) is accessible through the upper opening (16).

3. A box (10, 110) according to claim 1 or 2, wherein the closure means (18) is transparent.

4. A box (10, 110) according to any preceding claim, further comprising a removable insert (30) adapted to be disposed in the interior (14) of the box body (12), the insert (30) being adapted to receive a cell tissue culture (TC).

5. Box (10, 110) according to the preceding claim, in which the insert (30) is removable from the box body (12) through the upper opening (16).

6. A box (10, 110) according to claim 4 or 5, wherein the insert (30) has a growing area (34) and a gripping area (35) connected to the growing area (34), the gripping area (35) being adapted to manipulate the insert (30) relative to the box body (12).

7. Box (10, 110) according to the preceding claim, in which the culture area (34) includes an upper opening (44) adapted to accommodate the cell tissue culture (CT).

8. A box (10, 110) according to claim 6 or 7, wherein the growing area (34) includes a porous bottom (46).

9. Box (10, 110) according to the preceding claim, in which the porous bottom (46) is removable from the rest of the insert (30).

10. A box (10, 1100) according to claim 8 or 9, wherein the porous bottom (46) is arranged opposite the upper opening (44) when the insert (30) is arranged in the box body (12).

11. Box (10, 110) according to one of claims 8 to 10, in which the porous bottom (46) is made of polyester, polyethylene or polycarbonate.

12. Box (10, 110) according to one of claims 6 to 8, in which the insert (30) includes at least one connection (47) adapted to cooperate removably with the interior (14) of the box body (12).

13. A box (10, 110) according to any preceding claim, wherein the filter (26) is disposed in an inclined portion (38) of the interior (14) of the box body (12).

14. A box (10, 110) according to any preceding claim, wherein the interior (14) of the box body (12) includes a working area (36) adapted to receive the cell tissue culture (CT) and fluid culture media enabling the cell tissue (CT) to grow.

15. Box (10, 110) according to one of the preceding claims, wherein the box body (12) further comprises a partition (150) separating the working area (36) from the filter (26).

Images