Single-use filtration device for the purification of adipose tissue

Abstract

Single-use filtration device for the purification of adipose tissue The present invention relates to a single-use filtration device (100) for the purification of adipose tissue comprising at least: - a sealed enclosure (110), - a filter (120) present in the sealed enclosure, said filter delimiting a centrifugation chamber (160) for adipose tissue, - pipes (170-183) connected to the sealed enclosure, - a rotating plate (131) connected to the filter (120), the rotating plate being configured to cooperate with a rotation drive device (30). Figure for abstract: Fig. 1.

Description

Title of the invention: Single-use filtration device for the purification of adipose tissue Technical field

[0001] The present invention relates to a single-use filtration device for the purification of adipose tissue. The invention finds application in particular for fat transplantation operations (referred to as "lipofilling" in English) for aesthetic and reconstructive purposes. The invention finds, in particular, use for the transplantation of fat useful for breast surgery operations, the implementation of the device which is the subject of the invention not being limited to this application. Prior art

[0002] Fat transplantation or autologous adipose tissue grafting operations are used in surgery, particularly in buttock surgery for volume augmentation or in breast surgery to shape the breast to give it a more natural appearance after a DIEP (Deep Inferior Epigastric Perforator), latissimus dorsi flap reconstruction, or after the placement of a breast implant. These operations involve taking fat from a patient in a donor area and then reintroducing it into the area of ​​interest in the patient's body.

[0003] These operations are currently not reproducible and may require several interventions due to the resorption of the material introduced (loss of volume encountered after the operation). Indeed, the adipose tissue removed contains a liquid medium consisting mainly, on the one hand, of oil and blood initially present in the adipose tissue and, on the other hand, of a liquid such as a physiological solution introduced during the removal of the adipose tissue and / or during a washing operation thereof.

[0004] The inventors have determined that the resorption rate is largely related to the amount of liquid present in the adipose tissue reintroduced into the patient's body. In other words, the more liquid remains in the reinjected adipose tissue, the higher the resorption rate. It is therefore important to treat the adipose tissue before its reintroduction into the patient's body in order to remove as much of the liquid medium as possible. This treatment must nevertheless be carried out under controlled hygiene conditions in order to eliminate the risks of contamination from one patient to another.

[0005] Document US 2020 / 0054824 discloses a fat transplantation system that uses a gravity filtration device marketed under the reference com commercial Revolve®. However, this type of filtration device does not allow for the removal of a sufficient quantity of liquid from the adipose tissue to significantly reduce the resorption rate. Furthermore, in the system of document US 2020 / 0054824, the adipose tissue circulates in a pumping circuit that must be disinfected between two operations, which results in immobilization of the system after each transplantation. It is also difficult to control disinfection throughout the pumping circuit.

[0006] However, there is a need for a solution that allows optimal treatment to be carried out for the elimination of the liquid medium present in adipose tissue while ensuring very good hygiene conditions. Statement of the invention

[0007] To this end, the present invention provides a single-use filtration device for the purification of adipose tissue comprising at least: - a waterproof enclosure, - a filter present in the sealed enclosure, said filter delimiting a centrifugation chamber for adipose tissue, - pipes connected to the watertight enclosure, - a rotating plate connected to the filter, the rotating plate being configured to cooperate with a rotational drive device.

[0008] The filtration device of the invention thus advantageously combines the use of a filter capable of draining a liquid medium from adipose tissue with the rotation of the latter. Indeed, the filter forming a centrifugation chamber, adipose tissue present inside this chamber can be subjected to centrifugal accelerations against the internal wall of the filter. The liquid medium present in and around the adipose tissue is then effectively drained outside the centrifugation chamber through the pores of the filter while the adipose tissue is retained in the chamber. A significant quantity of liquid is thus eliminated, making it possible to have purified adipose tissue which, once reimplanted, has a resorption rate much lower than that obtained with the filtration solutions of the prior art.

[0009] Furthermore, the filtration device of the invention comprises pipes used for the circulation of the adipose tissue. The pipes being part of the single-use device, perfect hygiene is thus ensured at each intervention by replacing the elements in which the adipose tissue circulates or passes through.

[0010] In an exemplary embodiment, the purification device further comprises a stiffening element present between the sealed enclosure and the filter.

[0011] In another embodiment, the filter is made of a self-supporting rigid material. In this case, a stiffening element is no longer necessary in the pu- verification.

[0012] In an exemplary embodiment, the purification device further comprises a collection tray present in the centrifugation chamber, the collection tray being movable in translation along the axis of rotation of the filter. The collection tray acts as a piston which makes it possible to scrape the internal wall of the filter so as to collect a maximum of adipose tissue and to facilitate the collection of the purified adipose tissue at the top of the device.

[0013] According to another particular aspect, the purification device further comprises a threaded rod extending into the centrifugation chamber and cooperating with a threaded portion of the collection tray, the threaded rod being connected to the means for driving the filter in rotation, said means being configured to drive the threaded rod in rotation in a direction of rotation opposite to the direction of rotation of the filter. Here, the same rotational drive means is advantageously used both for centrifuging the filter and for moving the collection tray in vertical translation.

[0014] According to another particular aspect, the purification device further comprises a protective sheath surrounding the threaded rod. This sheath makes it possible to prevent the threaded rod from coming into contact with the adipose tissue which, by accumulating at the level of the threading of the plate, can block the movement of the latter.

[0015] The invention also relates to a machine for purifying adipose tissue from a patient, the machine comprising at least:

[0016] - a single-use filtration device according to the invention,

[0017] - a rotational drive device on which the filtration device for use single is removably attached, the rotary drive device comprising a rotary motor connected to the single-use filtration device,

[0018] - a suction pump connected to the single-use filtration device,

[0019] - a circulation pump connected to the single-use filtration device,

[0020] - a washing liquid tank connected to the single-use filtration device,

[0021] - a control unit configured to control the drive device in rotation, suction pump and circulation pump.

[0022] The machine of the invention makes it possible to carry out all the operations necessary (sampling, washing, centrifugation and reimplantation of adipose tissue) for an adipose tissue transplantation treatment, and this in very good hygienic conditions for each treatment thanks to the use of a single-use filtration device.

[0023] In an exemplary embodiment, the machine further comprises a pinch valve device cooperating with pipes of the single-use filtration device, the control unit being configured to control said pinch valve device so as to selectively prevent circulation in one or more pipes.

[0024] The pinch valve device allows selective control of the circulation in the pipes while facilitating the assembly and disassembly of the pipes when replacing the single-use filtration device.

[0025] In an exemplary embodiment, the rotation drive device comprises a stator flange, the sealed enclosure of the single-use filtration device comprising removable fixing means capable of locking said sealed enclosure in rotation on the stator flange.

[0026] It is thus possible to quickly and easily lock and unlock a single-use filtration device on the machine. Brief description of the drawings

[0027] [Fig-1] [Fig.l] is a schematic perspective view of a machine for puri formation of adipose tissue in accordance with one embodiment of the invention.

[0028] [Fig.2] [Fig.2] is an exploded schematic view of a single-use filtration device in accordance with an exemplary embodiment of the invention,

[0029] [Fig.3] [Fig.3] is a schematic sectional view of the single-use filtration device of [Fig.l] once assembled,

[0030] [Fig.4] [Fig.4] is a schematic sectional view showing the single-use filtration device of [Fig.2] after moving the collection tray,

[0031] [Fig.5] [Fig.5] is a schematic sectional view showing the single-use filtration device of [Fig.2] before attachment to a rotating drive device of the machine for purifying adipose tissue,

[0032] [Fig.6] [Fig.6] is a schematic sectional view showing the single-use filtration device of [Fig.2] after attachment to a rotating drive device of the machine for purifying adipose tissue,

[0033] [Fig.7] [Fig.7] is a detail view of a pinch valve device of the machine for purifying adipose tissue of [Fig.l]. Description of the embodiments

[0034] [Fig. 1] illustrates an example of a machine 1 according to the invention for the purification of adipose tissue. In general and as will be detailed further below, the machine 1 comprises a control unit 10 allowing the implementation of the different treatment steps, a circulation pump 20 and a suction pump 60 controlled by the control unit 10, a rotation drive device 30 controlled by the control unit 10.

[0035] The machine 1 further comprises a single-use filtration device 100 for the purification of adipose tissue in accordance with one embodiment of the invention, the filtration device 100 being removably mounted on the machine 1.

[0036] As illustrated in Figures 2 and 3, the single-use filtration device 100 comprises in particular:

[0037] - a waterproof enclosure 110,

[0038] - a filter 120 present in the sealed enclosure 110, said filter delimiting a chamber 160 centrifugation for adipose tissue,

[0039] - several pipes 170 to 173 connected to the sealed enclosure 110,

[0040] - a rotating plate 131 in connection with the filter 120, the rotating plate being configured to cooperate with the rotation drive device 30.

[0041] The sealed enclosure 110 is formed here by a cover 111, a cylindrical wall 112 and a bottom 113. These elements are fixed together in a sealed manner.

[0042] The filtration device 100 also comprises a filter or screen 120 present in the sealed enclosure 110. The filter 120 delimits a centrifugation chamber 160 ( [Fig. 3]) whose operation is described later. In the example described here, the filter 120 has a cylindrical shape. The filter may have other shapes suitable for centrifugation. The filter 120 has a pore size configured to allow a liquid medium to pass through and retain adipose tissue. The pore size is chosen in particular to allow the passage of liquids such as oil, blood, water or a physiological solution while retaining the adipose tissue. As non-limiting examples, the pore size of the filter may be between 50 μm and 1500 μm and more preferably between 200 μm and 500 μm.The filter can be made in various ways, such as by braiding, welding plastic threads or from a material comprising openings or holes having dimensions corresponding to the desired pore size. The filter can be made in particular of a polymeric material such as polyester or polypropylene, but those skilled in the art will recognize that other materials can be used.

[0043] In the example described here, a stiffening element 130 of cylindrical shape is present between the cylindrical wall 112 of the sealed enclosure 110 and the filter 120. Its function is in particular to ensure the structural strength of the filter 120 during centrifugation. The stiffening element 130 is for example made of metallic or plastic material and has an openwork structure defining a plurality of openings 1300 in order to allow the evacuation of the liquid medium drained by the filter 120.

[0044] The stiffening element 130 is associated with a rotary plate 131. More precisely, the stiffening element 130 has at its lower end teeth 1301 which cooperate with grooves 1310 present in the vicinity of the external periphery of the rotary plate 131. Of course, any other means of securing the stiffening element with the rotary plate, such as for example clipping or gluing, can be envisaged. The rotary plate 131 is connected to the rotation drive device 30. The rotational drive device comprises a rotary electric motor 300. The electric motor 300 may be, for example, a stepper motor or a brushless DC motor. In the example described here, the rotary plate 131 is connected to the rotary electric motor 300 by a bidirectional clutch 350 configured to ensure, via a first shaft 351, the rotational drive of the rotary plate 131 in a first direction of rotation Si of the motor 30 ([Fig. 3]). The bidirectional clutch may be replaced by any device allowing selective rotational drive in two opposite directions of rotation. Seals 114 and 115 are placed respectively below and above the rotary plate 131 in order to ensure sealing in the lower part of the purification device.

[0045] Since the filtration device 100 is disposable, it is mounted interchangeably on the machine. For this purpose and as illustrated in FIGS. 3 to 6, the rotation drive device 30 comprises a stator flange 310 on which the bottom 113 of the sealed enclosure 110 is fixed. In the example described here, a bayonet-type fixing is used which makes it possible both to secure the disposable filtration device 100 with the stator flange 310 of the rotation drive device 30 of the machine 1 and to lock the sealed enclosure 110 of the device in rotation. More specifically, the bottom 113 of the sealed enclosure 110 comprises a fixing ring 1130 which has two notches 1133 and 1134 which cooperate with two tenons 311 and 312 present on the stator flange 310. It is thus possible to quickly and easily lock and unlock a single-use filtration device 100 on the machine 1.Any other removable fastening system can be used to secure the single-use filtration device to the rotation drive device 30 and to prevent the sealed enclosure 110 of the single-use filtration device 100 from rotating.

[0046] In the example described here, the rotary plate 131 comprises a finger 1312 which extends from the lower face 131b of the rotary plate and which is intended to cooperate with another finger 3510 present on the end of the first shaft 351 connected to the rotary motor 30 when the filtration device 100 is mounted on the machine ([Fig.6]). Any other system allowing coupling of the rotary drive device 30 with the rotary plate 31 can be used.

[0047] The centrifugation is carried out by rotating the filter 120. More precisely, the electric motor 300 is controlled by the control unit 10 in a first direction of rotation Si to drive the rotating plate 131 and the stiffening element 130 into engagement with the plate 131. The rotation of the plate 131 and the stiffening element 130 causes the filter 120 to rotate. The filter is fixed to the stiffening element by any type of suitable means. By way of non-limiting examples, the filter may be fixed with one or more of the following means: gluing, engagement in setting notches present on the stiffening element, clipping. The speed of the electric motor 30 is controlled by the control unit 10 so as to apply a centrifugal acceleration to the adipose tissue present in the centrifugation chamber. Thus, an adipose tissue present in the centrifugation chamber 160 will be subjected to a centrifugal force against the internal wall of the filter 120, which makes it possible to effectively drain the liquid medium present in the adipose tissue without damaging it.

[0048] The single-use filtration device comprises at least four pipes 170, 171, 172 and 173, the pipes 170, 171 and 172 being connected respectively to the ports 1110, 1111, 1112 present on the cover 111 of the sealed enclosure 110 while the pipe 173 is connected to an evacuation port 1131 present on the bottom 113 of the enclosure 110. The other end of the pipe 170 is connected to a venting device 90 provided with a filter 91.

[0049] In the example described here as illustrated in [Fig.l], other pipes are used in order to form circulation circuits in the machine 1 allowing the different stages of a purification treatment of adipose tissue to be carried out.

[0050] More specifically, in the exemplary embodiment described here, the machine 1 further comprises:

[0051] - a pipe 174 joining the pipes 172 and 173 connected to the filtration device 100,

[0052] - a pipe 175 one end of which is connected to a suction cannula 50 intended to suction fatty tissue from a patient,

[0053] - a pipe 176, one end of which is connected to a suction pump 60, the pipe 176 opening into pipe 174,

[0054] - a pipe 177 connecting the pipe 175 and a pipe 184 to the pipe 171,

[0055] - a pipe 178 connecting the pipes 171 and 177 to pipes 180 and 179,

[0056] - the pipe 179, one end of which is connected to an injection cannula 55 intended to delivering an anesthetic and purified adipose tissue into the patient's body, the tube 179 being connected to the tubes 178 and 180,

[0057] - the pipe 180, one end of which is connected to the circulation pump 20, the pipe 180 being connected to pipes 178 and 179,

[0058] - a pipe 181, one end of which is connected to the circulation pump 20, the pipe 181 being connected to pipes 182 and 183,

[0059] - the pipe 182, one end of which is connected to a washing liquid reservoir 70, the pipe 182 being connected to pipe 181 and to a pipe 183,

[0060] - the pipe 183, one end of which is connected to a washing liquid reservoir and of anesthetic 80, the pipe 183 being connected to the pipes 181 and 182,

[0061] - a pipe 184 connecting the pipes 175 and 177 with the pipe 174.

[0062] All the pipes described above are for single use.

[0063] The machine 1 further comprises a pinch valve device or constriction 40 comprising a plurality of pinch modules 41 to 48 also called "pinch valve" capable of selectively pinching one or more pipes described above in order to prevent circulation and to release the pinch in order to allow this circulation. In the example described here and as illustrated in [Fig.7], the pinch modules 41 to 48 each comprise a housing 410 to 480 delimited between two plates 490 and 491 and in which a pipe is arranged. The pinch modules 41 to 48 each further comprise a finger 411 to 481 movable in the corresponding housing between a retracted position (open position of the module) in which circulation is allowed in the pipe present in the housing and a deployed position (closed position of the module) in which the finger pinches the pipe present in the housing thus preventing circulation in the pipe.

[0064] In the example described here:

[0065] - the pipe 184 is present in the housing 410 of the pinching module 41,

[0066] - the pipe 177 is present in the housing 420 of the pinching module 42,

[0067] - the pipe 174 is present in the housing 430 of the pinching module 43,

[0068] - the pipe 178 is present in the housing 440 of the pinching module 44,

[0069] - the pipe 179 is present in the housing 450 of the pinching module 45,

[0070] - the pipe 182 is present in the housing 460 of the pinching module 46,

[0071] - the pipe 183 is present in the housing 470 of the pinching module 47,

[0072] - the pipe 170 is present in the housing 480 of the pinching module 48.

[0073] The position of each pinching module is now briefly described as a function of the steps or sequences implemented by the machine 1.

[0074] In the anesthesia step, the control unit 10 places the pinching modules 45 and 47 in the open position allowing circulation in the pipes 179 and 183 and the pinching modules 41, 42, 43, 44, 46 and 48 in the closed position preventing circulation in the pipes 184, 177, 174, 178, 182 and 170. By actuation of the pump 20 by the control unit 10, a liquid 81 present in the washing liquid and anesthetic reservoir 80 is sucked in by the pipe 183. The liquid is conveyed into the pump 20 by the pipe 181 and is then discharged from the latter by the pipe 180 and the pipe 179 in order to reach the injection cannula 55 used for introduce the anesthetic into the patient's body.

[0075] In the step of removing adipose tissue from a patient's body, the control unit 10 places the pinching modules 42 and 43 in the open position allowing circulation in the pipes 177 and 174 and the pinching modules 41, 44, 45, 46, 47 and 48 in the closed position preventing circulation in the pipes 184, 178, 179, 182, 183 and 170. The actuation of the suction pump 60 by the control unit 10 makes it possible to draw a vacuum into the filtration device 100 via the pipes 176, 174, 173 and 172, which causes the adipose tissue to be sucked up by the suction cannula 50 and its routing into the filtration device 100 via the pipes 175, 177 and 171.

[0076] In the step of purifying the adipose tissue removed, the control unit 10 places the pinching modules 44 and 46 in the open position allowing circulation in the pipes 178 and 179 and the pinching modules 41, 42, 43, 45, 47 and 48 in the closed position preventing circulation in the pipes 184, 177, 174, 179, 183 and 170. By actuation of the pump 20 by the control unit 10, a liquid 71 present in the washing liquid reservoir 70 is sucked in by the pipe 182. The liquid is conveyed into the pump 20 by the pipe 181 and is then discharged from the latter by the pipe 180, the pipe 178 and the pipe 171 in order to reach the filtration device 100. During the introduction of the washing liquid into the filtration device 100, one or more centrifugation cycles are carried out as described above to separate the polluting materials. The evacuation of the washing liquid containing the polluting materials (water, blood, oil, etc.) of the adipose tissue is carried out by actuating the suction pump 60 and placing the pinching modules 41, 43 and 48 in the open position by the control unit 10. In this configuration, the washing liquid containing the polluting materials is sucked in through the discharge port 1131 of the filtration device 100 and then circulates in the pipes 173, 174 and 176 in order to be conveyed into a bin 61 present upstream of the suction pump 60. The placement of the module 48 in the open position allows the filtration device to be ventilated and thus facilitates the evacuation of the washing liquid containing the polluting materials. The purification treatment of the adipose tissue with the single-use device of the invention is then completed.

[0077] The pinch valve device 40 allows selective control of the circulation in the pipes while facilitating the assembly and disassembly of the pipes when replacing the single-use filtration device.

[0078] Any other device making it possible to selectively prevent the circulation of material in the pipes may be used to carry out the various sequences described above.

[0079] Optionally, the purification device may further comprise a collection tray movable inside the centrifugation chamber. As illustrated in the example of Figures 3 and 4, the purification device 100 further comprises a collection tray 140 which is movable in translation in a direction DT along the axis X of the filter 120. More specifically, the purification device 100 comprises a threaded rod 141 which extends vertically inside the centrifugation chamber 160 along the axis X of the filter 120. The lower end 1412 of the threaded rod is connected to the electric motor 10 via a guide 150 and the bidirectional clutch 20. The guide 150 comprises a first housing 1500 in to which the lower end 1412 is fixed. A lower portion 1501 of the guide 150 comprises a second housing 1502 which cooperates with a free end 3520 of a second shaft 352 of the bidirectional clutch 350 when the single-use filtration device 100 is mounted on the rotary drive device 30 ([Fig.6]).

[0080] The second shaft 352 is engaged with the electric motor 300 only when the latter transmits a rotational movement in a second direction of rotation S2 opposite to the first direction of rotation Si used for centrifugation. When the electric motor 300 rotates in the first direction of rotation Sb, no rotational movement is transmitted by the bidirectional clutch 350 to the guide 150 to which the threaded rod 141 is connected. Similarly, when the motor 300 rotates in the second direction of rotation S2, no rotational movement is transmitted by the bidirectional clutch 350 to the rotating plate 131 and, consequently, to the filter 120. The collection plate 140 has on its upper face a central opening 1400 extended by a neck 1401 which extends from the lower face of the plate 140. The neck 1401 comprises a portion 1402 comprising a thread 1403 which cooperates with a thread 1411 of the threaded rod 141.Thus, when the threaded rod is rotated in the second direction of rotation S2, the collection tray 140 rises in the centrifugation chamber 160 in the direction DT.

[0081] The peripheral edge 1404 of the collection tray 140 is opposite the internal wall of the filter 120. Thus, when the collection tray 140 is moved in vertical translation along the direction DT, it acts as a piston which makes it possible to scrape the internal wall of the filter so as to collect a maximum amount of adipose tissue. The vertical translation of the tray 140 also makes it possible to place the collected adipose tissue as close as possible to the cover 111 and therefore facilitate its removal for reimplantation.

[0082] According to a particular aspect, the threaded rod 141 can be housed in a protective sheath 142. The protective sheath 142 which extends between an upper end 1421 and a lower end 1422 makes it possible to prevent the threaded rod 141 from coming into contact with the adipose tissue which, by accumulating at the level of the tapping 1403 of the collection plate 140, can block the movement of the latter.

[0083] [Fig.4] shows the purification device 100 after actuation of the electric motor 10 in the second direction of rotation S2 allowing the threaded rod 141 to be driven in rotation. The rotation of the threaded rod 141 causes the vertical translation of the collection tray 140 in the direction DT.

[0084] The lower end 1422 of the protective sheath is fixed in the opening 1400 of the collection tray 140. In order to allow the movement of the protective sheath 142 during the movement of the tray 140, the hood 111 and the cover 101 comprise respectively an opening 1113 and an opening 1013 through which the sheath 142 slides. A seal 115 is present around the opening 1113 in order to preserve the seal at the top of the centrifugation chamber.

[0085] The collection tray can also be used in the step of reintroducing the purified adipose tissue into the patient's body. In this case, the collection tray acts as a piston that pushes the purified adipose tissue toward the upper part of the centrifugation chamber as close as possible to the port 1111. The control unit 10 places the pinching modules 44 and 45 in the open position allowing the circulation of the adipose tissue in the pipes 171, 178 and 179 and the pinching modules 41, 42, 43, 46, 47 and 48 in the closed position. The purified adipose tissue can thus be conveyed from the centrifugation chamber to the injection cannula 55.

[0086] In another embodiment, the filter is made of a self-supporting rigid material. The filter may in particular be made from a metal strip in which holes are made by laser, water jet or chemical cutting in order to form a screen, a metal grid woven from wires or balls agglomerated by sintering metal or ceramic powders. In this case, the stiffening element 130 is no longer necessary and it is the self-supporting filter which is directly engaged with the rotating plate 131.

Claims

Claims

1. Single-use filtration device (100) for the purification of adipose tissue comprising at least: - a sealed enclosure (110), - a filter (120) present in the sealed enclosure, said filter delimiting a centrifugation chamber (160) for adipose tissue, - pipes (170-183) connected to the sealed enclosure, - a rotating plate (131) connected to the filter (120), the rotating plate being configured to cooperate with a rotation drive device (30).

2. Device according to claim 1, further comprising a stiffening element (130) present between the sealed enclosure (110) and the filter (120).

3. Device according to claim 1, in which the filter is made of a self-supporting rigid material.

4. Device according to any one of claims 1 to 3, further comprising a collection tray (140) present in the centrifugation chamber (160), the collection tray being movable in translation along the axis of rotation of the filter.

5. Device according to claim 4, further comprising a threaded rod (141) extending into the centrifugation chamber (160) and cooperating with a threaded portion (1403) of the collection tray (140), the threaded rod being configured to cooperate with a rotational drive means.

6. A device according to claim 5, further comprising a protective sheath (142) surrounding the threaded rod (141).

7. Machine (1) for the purification of adipose tissue, the machine comprising at least: - a single-use filtration device (100) according to any one of claims 1 to 6, - a rotational drive device (30) on which the single-use filtration device (100) is removably fixed, the rotational drive device comprising a rotary motor (300) connected to the single-use filtration device, - a suction pump (60) connected to the single-use filtration device, - a circulation pump (20) connected to the single-use filtration device single, - a washing liquid tank (70) connected to the single-use filtration device, - a control unit (10) configured to control the rotation drive device (30), the suction pump (60) and the circulation pump (20).

8. Machine according to claim 7, further comprising pinch valve device (40) cooperating with pipes of the single-use filtration device (100), the control unit (10) being configured to control said pinch valve device so as to selectively prevent circulation in one or more pipes.

9. Machine according to claim 7 or 8, in which the rotation drive device (30) comprises a stator flange (310), the sealed enclosure (110) of the single-use filtration device (100) comprising removable fixing means capable of locking said sealed enclosure in rotation on the stator flange.

Images