Autofocus during video-microscopy

Abstract

Autofocusing an object 101 at a microscope 100 involves receiving, using a beam splitter 108, a portion of light from the object at a camera 102 and receiving a remaining portion of the light at a sensor 104. One or more images of the object are captured at the sensor which is inclined with respect to the orthogonal image plane, further wherein the images have a region of the highest level of focus, such that movements of the object result in a change of location of the region of the highest level of focus in the one or more images captured at the sensor. One or more images of the object are captured at the camera and a location of the region of the highest level of focus is determined in the images captured at the sensor. The image plane of the object to the camera is restored by adjusting a refocusing device 106, positioned between the object and the beam splitter, wherein the restoring is based on the determined location of the region of the highest level of focus on the sensor.

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