Biomimetic anti-aging skincare composition with prebiotic, postbiotic and pro-biotic enriched components

Abstract

The present invention relates to a biomimetic anti-aging skincare product in particular a beautification product with a skin nursing complex that comprises an aging resistance effect, characterised in

Description

The invention relates to the technical field of Biomimetic (Pre, Pro and Post Biotic Enriched) Anti-Aging Skincare products—the next frontier in organic and sustainable skincare repair face cream, in particular to Biomimetic Anti-Aging Cream and Serum (Pre, Pro and Post Biotic Enriched) preparation method thereof. BACKGROUND OF THE INVENTION Our skin is put through its paces every day. Our environment, variations in temperature, pollution, stress and fatigue all impact our skin quality. It becomes dehydrated and can even become uncomfortable. This is where biomimetism comes in. By imitating the skin's natural composition, it provides the skin with only high tolerance, 100% natural-origin ingredients. Biomimetism is inspired by the skin, imitating its natural protective power to create innovative skincare. Biomimetic skincare incorporates ingredients naturally found in skin such as water, lipids, proteins, sugars and mineral salts. Your skin is therefore naturally in harmony with these skincare products, it “accepts” them and knows how to use them to help replenish the skin's natural barrier. If it is broken down, the prefix “bio” refers to life, while “mimetic” is derived from the word “imitate.” Biomimicry literally means “imitation of the living,” and refers to a branch of science that studies nature and then imitates, or takes inspiration from, natural designs and processes to solve human problems. Biomimetic skin care is an advanced approach to formulation utilising innovative plant-based and synthetic biomimetic ingredients that integrate nature and science. These ingredients mimic skin structures and biochemicals which enable optimal delivery and results; this is the next frontier in natural skin care. The beauty industry has received backlash for conducting animal testing for their products to ensure that they are safe for human use. Biomimetic products don't need to, because, with natural ingredients, they're safe and harmless. Therefore, the customers buy cruelty-free skin care products and also supporting the move towards abolishing animal testing in the industry’. Market Assessment The global skin care products market size was valued at USD 130.50 billion in 2021 and is expected to expand at a compound annual growth rate (CAGR) of 4.6% from 2022 to 2030. Escalating demand for face creams, sunscreens, and body lotions across tire globe is expected to have a positive impact on the market growth over the forecast period. Moreover, the flourishing e-commerce sector is anticipated to boost market growth further. In the UK, revenue in the Skin Care segment amounts to US$4.05bn in 2023. The market is expected to grow annually by 2.38% (CAGR 2023-2027). The organic skincare market is evolving at a rapid pace evident in the growth statistics. The Global Organic Skincare Market size was valued at USD 9.83 billion in 2021 and is poised to grow from USD 10.49 billion in 2022 to USD 21.16 billion by 2030, growing at a CAGR of 8.9% in the forecast period (2023-2030). The revenue of the natural and organic cosmetics market in the United Kingdom amounted to around 221 million British pounds in 2020. That same year, the average revenue per capita stood at 3.25 British pounds. Analysts note that millennials are a key target demographic for organic skincare brands. This generation is more likely to be concerned about environmental issues and their own personal health, making them more likely to purchase organic skincare products. Biomimetic skincare is an emerging approach to skincare formulation that sits at the intersection of cutting-edge scientific technology and nature. “These are synthetic or natural materials that can mimic our own skin’s natural function,” explains Ginger King, cosmetic chemist. “Biomimetic materials [are] triggers for our own natural production of collagen, elastin, sebum, etc., instead of applying materials like collagen on skin, which is useless.” Biomimicry has been the catalyst for the discovery of powerful anti-aging peptides, how to reignite slowed collagen production, and superior moisturizing ingredients. Biomimetic skincare doesn’t refer to specific ingredients so much as it refers to a framework from which ingredients and formulas are derived. It’s also more sustainable. Natural ingredients aren’t always the most sustainable, and scientists can use biomimetics to formulate new alternatives that are more sustainable with less environmental impact. Thus, the future of beauty lies in the past - millions of years of evolution across all species. Time has proven that nature has always been the cure - biomimetics just harnesses that cure and distils that knowledge into practical applications that solve real, human problems. Also, because biomimetic products use naturally grown ingredients that are free from toxic pesticides and fertilizers, they don't leave a harmful footprint on the planet, particularly the soil, water, and air. Given the growing market demand for organic products and the emerging trend of biomimetics particularly in the skin care industry offers a progressive scope of skin products that improves the health of skin giving it a long-lasting natural impact. BRIEF DESCRIPTION OF THE DRAWINGS Implementations of the present technology will now be described, by way of example only, with reference to the attached figures Fig. 1 illustrates the Skin elasticity (R2) increase percentage; Fig 2 illustrates the Fatigue Resistance (F4) decrement percentage; Fig. 3 illustrates the Cell growth increase percentage; Fig 4 illustrates the Cell Proliferation rate percentage. Fig. 5 illustrates the HABP straining of the vehicle-treated (VEH) human skin explant tissues and the 0.04% stabilized-biomimetic complex (BMC) -treated skin explant tissues; Fig 6 illustrates the comparison between the epidermis thickness of the vehicle-treated tissue and tire epidermis thickness of the stabilized-biomimetic complex (BMC); Fig 7 illustrates the present Biomimetic anti-aging cream that employs a barrier lesion on a 3D skin model. DETAILED DESCRIPTION OF THE INVENTION It will be appreciated that for simplicity and clarity of illustration, where appropriate, reference numerals have been repeated among the different figures to indicate corresponding or analogous elements. In addition, numerous specific details are set forth in order to provide a thorough understanding of the exemplary embodiments described herein. However, it will be understood by those of ordinary skill in the art that the exemplary embodiments described herein can be practiced without these specific details. In other instances, methods, procedures, and components have not been described in detail so as not to obscure the related relevant feature being described. The drawings are not necessarily to scale and the proportions of certain parts may be exaggerated to better illustrate details and features. The description is not to be considered as limiting the scope of the exemplary embodiments described herein. The term “comprising" means “including, but not necessarily limited to”; it specifically indicates open-ended inclusion or membership in a so-described combination, group, series, and the like. Product Composition for Biomimetics Anti-ageing Cream and Serum The cream and senim are designed to balance the skin microbiome, reduce age spots and discoloration, and provide ultra-hydration and ultra-brightening using biomimetics. The proposed unique products contain prebiotic, postbiotic, and probiotic ingredients in such a unique and optimized ratio that it balances and nourish the skin microbiome (trillions of microorganisms, like bacteria, fungi, and viruses found on the skin as invisible lifeforms are known as the skin microbiome). The company has conducted extensive research on the most effective delivery systems for probiotics, live bacteria and postbiotic components in Biomimetic skincare products to ensure maximum effectiveness and stability. All compositions in the skincare formulations have some unique proportion to offer which determines its efficacy and activity. Our Optimized mix of natural prebiotics, probiotics, and postbiotics ingredients derived from natural processes such as fermentation and other organic sources that balance and nourish the skin microbiome. Our invention revolves around the concept of imitating natural process of cell turnover with the natural ingredients. To achieve this goal, we took ingredients from nature which are easily available and sustainable. It has also incorporated additional ingredients, such as antioxidants and peptides, in the unique Biomimetic formulation of anti-aging skincare. Aiming at the defects of the prior art, the invention provides biomimetic anti-aging face cream and serum preparation method thereof, and solves the problems in the background art. In order to achieve the above purpose, the invention is realized by the following technical scheme: Method for preparing the beautification product of the skin nursing complex that comprises the aging resistance effect is characterized in that: A) one or more oils and active component Centella asiatica (Gotu Kola) Flower / Leaf / Stem Extract, Rosa canina (Rosehip) Fruit Oil are to mix under 45-50 °C with water to form first mixture; Subsequently, B) be 1 by first mixture that a) makes, independent of additional mixture that makes with the adding speed adding that is no more than lOg / min under the OOOrpm, defined second mixture is to be made of phospholipid and optional further adjuvant and active substance; C) the 3rd mixture that forms therefore is to mixing under 600-800rpm, until making water-oil emulsion; D) combine water and gellant, and be no more than l,200rpm stirs down, till forming gel network; With E) the 3rd mixture is poured in the gel of step d) in 15 40-ininute time under the 700rpm at minimum; F) according to e) gel that we get by with cosmetics adjuvant, active substance or its mixture at 25-35 °C be less than under the 700rpm and mix. The present invention is use for averting or reducing the appearance of human skin aging of a beauty composition. Herein, the averting or reducing of the appearance of human skin aging is with respect to a distinguishingly selected from the group consisting of wrinkles, evening skin Colour, evening tone and combinations thereof. The use of an Undaria pinnatifida Cell Culture Extract in the manufacture of a medicament for reducing skin irritation or sting caused by hydroxy acid. Herein, the Undaria pinnatifida Cell Culture Extract is present in an amount of from 1 % by weight of the medicament. Biomimetic (Pre, Pro and Post Biotic Enriched) anti-aging cream and serum into a distillation flask, distilling for 3-5 hours by using steam at a distillation speed of 1-2 drops per second, and finally adding into the stirrer; stirring and dispersing the emulsion, adding an antioxidant, a chelating agent, panthenol and a plant extract, uniformly stirring, and standing and cooling for 5-8 hours; step six, cooling, using a transparent plastic plate to remove surface foam, putting the mixture into a heating container, adding an emollient, a humectant, a peptide active substance and an antioxidant, uniformly stirring, and then standing for cooling; and seventhly, adding a proper amount of water, a chelating agent, panthenol and a plant extract after cooling, and uniformly stirring to obtain the biomimetic anti-aging cream and serum. Description of the embodiments The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. Examples 1 series A Biomimetic anti- ageing face cream and serum preparation method thereof, wherein the Biomimetic anti- ageing face cream and serum comprises upto 30 parts of water, Biomimetic mixture of Pre, post and pro Biotic components upto 15 parts, 15 parts of adenosine, 5 parts of tocopheryl acetate, 2.5 parts of propylene glycol glycerol, 3.5 parts of calcium pantothenate, 2.5 parts of ceramide, 0.5 part of nicotinamide, 55 parts of provitamin B, 3.5 parts of amino acid humectant, 33.5 parts of vitamin B, 1 part of amino acid humectant, 1 part of shea butter, 1 part of urea, 4 parts of peptide active substance, 1 part of polysaccharide substance, 2 parts of humectant, 100 parts of emollient, 2 parts of emulsifier, 0.25 part of antioxidant, 0.02 part of chelating agent, 50 parts of deionized water, 20.5 parts of caprylic / capric triglyceride, 6.5 parts of propylene glycol, 3.25 parts of cetylstearyl oleate, 2.25 parts of sorbitan oleate, 2.2 parts of squalane, 2 parts of tocopheryl acetate, 7.49 parts of glycerol, 1.5 parts of shea butter, 2 parts of urea, 1.3 parts of sodium hyaluronate, 3.3%of vitamin B, 3.003%of glucosamine, 0.0.003% of calcium pantothenate, and 0.0.003% of lactic acid amide in parts by weight: Method for preparing the beautification product of the skin nursing complex that comprises the aging resistance effect is characterized in that: A) one or more oils and active component Centella asiatica (Gotu Kola) Flower / LeafiStem Extract, Rosa canina (Rosehip) Fruit Oil are to mix under 45-50 °C with water to form first mixture; Subsequently, B) be 1 by first mixture that a) makes, independent of additional mixture that makes with the adding speed adding that is no more than lOg / min under the OOOrpm, defined second mixture is to be made of phospholipid and optional further adjuvant and active substance; C) the 3rd mixture that forms therefore is to mixing under 600-800rpm, until making water-oil emulsion; D) combine water and gellant, and be no more than l,200rpm stirs down, till forming gel network; With E) the 3rd mixture is poured in the gel of step d) in 15-40-minute time under the 700rpm at minimum; F) according to e) gel that we get by with cosmetics adjuvant, active substance or its mixture at 25-35 °C be less than under the 700rpm and mix. The present invention is use for averting or reducing the appearance of human skin aging of a beauty composition. Herein the averting or reducing of the appearance of human skin aging is with respect to a distinguishingly selected from the group consisting of wrinkles, evening skin Colour, evening tone and combinations thereof. The use of an Undaria pinnatifida Cell Culture Extract in the manufacture of a medicament for reducing skin irritation or sting caused by hydroxy acid. Herein the Undaria pinnatifida Cell Culture Extract is present in an amount of from 1 % by weight of the medicament. Examples 2 Series The Biomimetic anti- ageing face cream and serum preparation method thereof are characterized in that: according to the weight ratio, the Biomimetic anti- ageing face cream and serum comprises upto 40 % of water, Biomimetic mixture of Pre, post and pro Biotic components upto 15 parts, 35% of adenosine, 7% of tocopherol acetate, 6.5% of propylene glycol glycerin, 3% of calcium pantothenate, 4% of ceramide, 3% of nicotinamide, 8% of provitamin, 3% of amino acid humectant, 3% of vitamin, 3% of shea butter, 4% of urea, 25% of peptide active substance, 6% of polysaccharide substance, 30% of humectant, 20% of emollient, 10% of emulsifier, 3% of antioxidant, 0.6% of chelating agent, 90% of deionized water, and 20.5% of capryl ic / capric triglyceride, 6.5% of propanediol, 3.25% of cetostearyl oleate, 2.25% of sorbitan olive oleate, 2.2% of squalane, 2% of tocopherol acetate, 7.49% of glycerin, 1.5% of shea butter, 2% of urea, 1% of sodium hyaluronate, 30.3% of vitamin B, 0.2% of acetylglucosamine, 0.1% of calcium pantothenate, 0.075%, 0.01% of adenosine, 0.003% of ceramide, and 0.003% of ceramide according to the weight ratio: Method for preparing the beautification product of the skin nursing complex that comprises the aging resistance effect is characterized in that: A) one or more oils and active component Centella asiatica (Gotu Kola) Flower / LeafiStem Extract, Rosa canina (Rosehip) Fruit Oil are to mix under 45-50 °C with water to form first mixture; Subsequently, B) be 1 by first mixture that a) makes, independent of additional mixture that makes with the adding speed adding that is no more than lOg / min under the OOOrpm, defined second mixture is to be made of phospholipid and optional further adjuvant and active substance; C) the 3rd mixture that forms therefore is to mixing under 600-800rpm, until making water-oil emulsion; D) combine water and gellant, and be no more than l,200rpm stirs down, till forming gel network; With E) the 3rd mixture is poured in the gel of step d) in 15-40-minute time under the 700rpm at minimum; F) according to e) gel that we get by with cosmetics adjuvant, active substance or its mixture at 25-35 °C be less than under the 700rpm and mix. The present invention is use for averting or reducing the appearance of human skin aging of a beauty composition. Herein the averting or reducing of the appearance of human skin aging is with respect to a distinguishingly selected from the group consisting of wrinkles, evening skin Colour, evening tone and combinations thereof. The use of an Undaria pinnatifida Cell Culture Extract in the manufacture of a medicament for reducing skin irritation or sting caused by hydroxy acid. Herein the Undaria pinnatifida Cell Culture Extract is present in an amount of from 1 % by weight of the medicament. The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention. Anti- Aging Biomimetic Complex Efficacy evaluation The test method was carried out by connecting an elastic probe PVM600 manufactured by Courage- Khazaka, German} (CK, Germany) to an MPA master according to a standard method and using the probe in combination with Cutomer Q analysis software. Working principle of skin elasticity test probe The test principle is based on the principle of suction and stretching, where a negative pressure is generated on the skin surface to be tested to suck the skin into a specific test probe, and the depth of the skin sucked into the test probe is measured by a non contact optical test system. The test probe includes a light emitter and a light receiver, the ratio of light (the ratio of emitted light to received light) is proportional to the depth of skin being absorbed, thus obtaining a curve of the length of skin stretched versus time, which is then analyzed by the MPA software to determine the elastic properties of the skin. Technical parameter index of skin elasticity test probe Host physical size: 27 X 25.5 X7 cm, probe: phi 2X10cm Probe test hole diameter: phi 2mm Pressure: (20-500) mbar Test time: (0.1-60) seconds Precision: penetration depth of about 3% above lOOum, and wireless property below lOOum Power supply: 100 to 240V / 50-60Hz / 0.3A Interface: USB (universal serial bus) Test negative pressure mode: continuous negative pressure mode Curve display mode: stretch length-time table Skin elasticity test mode-maintaining constant negative pressure Uf=Ue+Uv Wherein: uf-maximum skin stretches with negative pressure. Ue — the amount of skin stretches at 0.1 second after a constant negative pressure is applied to the skin, the amount of stretch in the elastic portion is located. Uv-Uf-Ue is the amount of stretching of the viscoelastic part, or plastic part, of the skin. The value of Ue is higher for skin that is more elastic than for younger skin, whereas the value of Ue is lower for skin that is less elastic than for older skin, and the value of Uv is higher for viscoelastic. Ur=Uf-Ul.l Ua=Uf-U2.0 Wherein: Ur-the value of elastic part Ur, i.e. the value of elastic part and the value of viscoelastic part, also called plastic part, after negative pressure is removed for 0.1 second. Ua-the recovery value of the skin from the removal of negative pressure to the next successive test of the skin surface plus negative pressure. The younger the skin, the more elastic the skin, the higher the elastic fraction value Ur; the older the skin, the less elastic the skin, the lower the Ur value. Skin elasticity test method Opening the Curometer Q software, clicking the tool bar Measurement or directly clicking the upper right Measurement button, taking down the probe cover after the pump is started, clicking OK, vertically and lightly contacting the probe with the skin area to be tested within 30 seconds (the skin surface should be cleaned, if a product is smeared, the test is carried out after the product is absorbed, so as not to pollute the probe hole), starting the test until the test cycle time is over, moving the probe away, and covering the probe cover. The 3-pomt test was performed in the area 2.5 X 2.5cm from the arm's upper ami wrist, and the average value of R2 was calculated, R2 = Ua / Uf. Meanwhile, the anti-fatigue degree value F4 of the skin can be calculated according to the measured data. Then, after the area was covered with a mask cloth, 0.45ml of the sample to be tested was injected into the film cloth, the film cloth was removed after applying for 20 minutes, the mask liquid was washed off after waiting for 10 minutes, and the skin elasticity value R2 and the fatigue resistance degree value F4 of the skin were measured after the skin was dried. Fibroblast assay Cell counting method After cell suspension preparation, cells are stained with the vital dye trypan blue for cell counting. Trypan blue is unable to penetrate the normal intact cell membrane of living cells, so living cells are not stained. The cell membrane permeability of the dead cells is increased, and the dye enters the cells to stain the cells (blue). MTT staining MTT is named as 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2-H-tetrazolium bromide, and the Chinese chemical name is 3- (4, 5-dimethylthiazole-2) -2,5-diphenyl tetrazolium bromide, the trade name is: thiazole blue, a yellow dye. The MTT method is also called MTT colorimetric method, and is a method for detecting cell survival and growth. The detection principle is that succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT into water-insoluble blue- purple crystalline Formazan (Formazan) and deposit the blue-purple crystalline Formazan in the cells, and dead cells do not have the function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its light absorption value is measured at 540 or 720nm wavelength by ELISA detector, which can indirectly reflect living cell number. Within a certain range of cell number, MTT crystals are formed in an amount proportional to the cell number. Test materials and methods Experimental materials DMEM, DPBS, Typsin-EDTA, Petri dish, 96well dish, 0.4% trypan blue solution, absolute ethyl alcohol or 95% ethyl alcohol solution, common microscope, cell counting plate, pipette, MTT, DMSO The experimental method comprises the following steps: Fibroblast cell culture Treating cultured fibroblast with Typsin-EDTA, collecting, suspending with DMEM, counting with a hemocytometer, and diluting to 5 * 10 cell concentration4cells / ml for use. Tire prepared cell suspension was aliquoted into 96well dish and 6well dish, respectively, and cultured, wherein the inoculation amount of 96well dish was lOOul, and the inoculation amount of 6well dish was 2 ml. The cells were incubated at 37 °C in an incubator containing 5% CO2 for 24 hours. Sample addition (1) Diluting a sample to be detected by using a DMEM culture medium, wherein the diluted concentrations are as follows: 1% (this concentration passes the previous MTT test and is non-toxic) (2) After 24 hours of cell culture, the previous DMEM was removed, and then carefully washed with DPBS (3) the DMEM medium to which the sample to be tested was added prepared in the first step is added in order. The cells were incubated at 37 °C in an incubator containing 5% CO2 for 48 hours. Cell counting Counting plate treatment Wiping the counting plate with absolute ethyl alcohol or 95% ethyl alcohol solution, wiping the counting plate with silk cloth, wiping another cover glass sheet, and covering the cover glass on the counting plate. Dyeing process Die cells cultured in 6well dish were removed from the medium, digested with typsin-EDTA, collected and suspended in DMEM medium. lOul of 0.4% trypan blue stain and lOul of cell suspension were pipetted with a pipette and mixed well. Slowly injecting from the edge of the counting plate to fill the gap between the counting plate and the cover plate. The counting plate was placed under a low power mirror (1 OX 10 times) to observe the counting. Counting method The number of cells in the four large squares (16 small squares per large square) of the counting plate was counted as shown. When counting, only intact cells are counted, and if cells are aggregated, the cells are counted as one cell. In a large square, if any cells are on-line, the off-line cells are generally counted without the on-line cells, and the left-line cells are counted without the right-line cells. Under the observation of the microscope, the cells with strong refractivity and no coloration are live cells, and the cells with blue coloration are dead cells. Conversion of the count After counting, the number of cells per ml of suspension was converted. Since the area of each square in the counting plate is 0.01cm2 Height of 0.01cm, so that its volume is 0.0001cm3I.e. 0.1mm3. Since 1ml is 1000mm3Therefore, the number of cells in each large square * 10000 = cell number / ml, and thus can be calculated as follows: cell suspension cell number / ml-4 big lattice cell total number / 4 x 10000 If the sample is diluted before counting, the dilution factor can be multiplied. After counting the cells, the concentration of the cells in the cell suspension is calculated. The cell proliferation rate of each sample was calculated based on the blank. MTT staining The MTT powder stored in the frozen state was dissolved in DPBS to prepare a solution having a concentration of 2 mg / ml. After 48 hours of culture in 96well dish, 50ul / well of MTT solution was added after removing the medium Culturing at 37 deg.C in 5% CO2 incubator for 3 hr MTT solution was completely removed after 3 hours After the MTT solution was completely removed, lOOul / well of DMSO solution was added to each well to dissolve formazan blue-violet crystal (formazan crystal) Absorbance was measured at 595 nm. The cell proliferation rate of each sample was calculated based on the blank Biopsy study design This was a pilot investigation study conducted in accordance with the principles of the 2013 Declaration of Helsinki and approved by an independent institutional review board. The purpose of investigation was to evaluate the histochemical skin changes after a stabilized biomimetic complex use. A total of 13 subjects were enrolled in the study. Informed consent was obtained from all individual participants included in the study. The subjects between the ages of 40 and 55, with Fitzpatrick skin type I-III, in good general health and with mild-to-moderate photo-damaged skin were recruited for the study. Subjects were excluded if they had used topical anti-aging within 30 days of study entry, or topical prescription retinoids within 90 days, or systemic retinoids within 6 months. The subjects were instructed not to use any products throughout the study. Paired tissues from the same individual were treated either by a stabilized- biomimetic complex or by its corresponding vehicle daily by randomization. The subjects used a 0.1% stabilized biomimetic complex lotion and its corresponding vehicle once daily in tire evening for 52 weeks. At end of treatment with the respective formulations, 2-mm punch biopsies were extracted from the subjects. The samples were later shipped to a pathology lab (AIMS Delhi) in 10% buffered neutral formalin for paraffin embedding and for HABP staining process. Results and Summary Discussion Skin Elasticity Test Name R2 increase % Blank -4.09 CG 1 0.53 CG2 0.29 CG3 0.79 CG4 0.92 CG5 0.92 CG6 1.42 CG7 2.84 CG8 1.89 EG 1 4.34 EG 2 3.3 EG 3 4.3 Fatigue Resistance (F4) Test Name F4 decrement (%) Blank 3.97 CG 1 3.47 CG2 4.46 CG3 11.8 CG4 11.46 CG5 15.76 CG6 15.35 CG7 13.73 CG8 10.21 EG 1 18.94 EG 2 17.95 EG 3 20.33 The CK probe is used for evaluating the skin elasticity and the fatigue resistance, the skin careful composition has obvious advantages in the aspects of timely improving the skin tightness and the fatigue resistance, the skin elasticity can be improved by 3.3-4.34% by smearing a sample for 30min, and the skin fatigue resistance is reduced by 17.95-20.33%. Results of cell counting experiments Name Cell Growth (%) CG 1 20.7 CG2 21.31 CG3 21.75 CG4 23.81 CG5 38.24 CG6 43.4 CG7 45.17 CG8 32.42 EG 1 58.99 EG 2 48.28 EG 3 58.88 MTT staining test results Name Cell proliferation Rate (%) CG 1 17.76 CG2 16.82 CG3 17.76 CG4 23.36 CG5 36.45 CG6 42.99 CG7 48.6 CG8 36.17 EG 1 64.49 EG 2 50.47 EG 3 63.55 The skin refining composition is applied to fibroblasts, and through cell counting and MTT chromosome evaluation, the skin refining composition provided by the invention has the advantages that the number of the fibroblasts can be remarkably increased to 48.28-58.88%, and the proliferation rate of the fibroblasts is 50.47% -64.49%. Topical stabilized Biomimetic Anti-Aging Treatment induces the expression in human skin To further confirm the effect of a stabilized Biomimetic Complex on HA production, we performed studies on full-thickness human skin explants. The HABP staining of three representatives’ images of the vehicle-treated (VEH) human skin explant tissues and the 0.04% stabilized-biomimetic complex (BMC) -treated skin explant tissues were shown in below figure. The HABP staining was in a diffuse pattern in the extracellular space between the epidermal keratinocytes and the dermal fibroblasts as shown in purple color with no nuclear counter-staining. Topical application of the stabilized biomimetic complex at 0.04% for 7 days showed darker purple color of HABP staining in epidermis and in dermis of the skin explants than that of the vehicle-treated skin explants, suggesting a stabilized-biomimetic complex formulation enhanced HA production in human skin explants. Paired tissues from each individual were treated either by the vehicle or a stabilized-BMC formulation daily for 52 weeks. The HABP staining of representative epidermal images of three pairs of the vehicle-treated tissues and the stabilized-BMC-treated tissues was shown in figure below. The HABP staining was in a diffuse pattern in the extracellular space between the epidermal keratinocytes as shown in blue color. Topical application of a stabilized-BMC (0.1%)-treated tissues showed darker blue color of HABP staining in the extracellular space of the viable layers of the epidermis as indicated by arrows (—»), which suggested that the stabilized-BMC formulation induced the production of HA in tire extracellular space of the viable layers of the epidermis as compared to that of the vehicle-treated tissues figure below. The HABP-staining intensities of the paired tissue of the vehicle- and the stabilized-BMC treatments were quantitated, as shown in figure below. The HABP staining of the paired tissues of the stabilized-BMC treatment was significantly higher than that of the vehicle treatment (p = 0.0076). These histological results demonstrate that a stabilized retinol formulation can continue to induce biological responses and sustain increased levels of HA in the epidermis in vivo over a year of continuous use. The epidermal thickness of the treated tissues was also quantitated. To confirm the efficacy of the stabilized BMC, the epidermis thickness of the treated samples was measured. The epidermis thickness of the stabilized-BMC-treated tissue is 117± 5 qm. whereas the epidermis thickness of the vehicle treated tissue is 83 ± 4 pm. Ure stabilized- BMC treatment increased the epidermal thickness as compared to that of the vehicle-treated tissues figure below. FIG7. shows that the present Biomimetic Anti- Aging Cream employs a barrier lesion on 3D skin model Thus, the proposed products will be a breakthrough in technology and formulation of natural skin care products using biomimetics (by using such natural ingredients that mimic to those actually existing in the skin for improving its appearance and immunity) that will help the skin in improving its self-defense mechanism and capacity to maintain its heath rather than getting support form external synthetic materials causing harm in the long run. The proposed formulations only express several combinations of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. By imitating the skin's natural composition, it provides the skin with only high tolerance, 100% natural-origin ingredients. Biomimetic skincare incorporates ingredients naturally found in skin such as water, lipids, proteins, sugars and mineral salts. Your skin is therefore naturally in harmony with these skincare products, it “accepts" them and knows how to use them to help replenish the skin's natural barrier. Biomimetic skin care is an advanced approach to formulation utilising innovative plant-based and synthetic biomimetic ingredients that integrate nature and science. These ingredients mimic skin structures and biochemicals which enable optimal delivery and results; this is the next frontier in natural skin care. “Biomimetic materials [are] triggers for our own natural production of collagen, elastin, sebum, etc., instead of applying materials like collagen on skin, which is useless.” Biomimiciy has been the catalyst for the discovery of powerful anti-aging peptides, how to reignite slowed collagen production, and superior moisturizing ingredients. Given the growing market demand for organic products and the emerging trend of biomimetics particularly in the skin care industry offers a progressive scope of skin products that improves the health of skin giving it a long-lasting natural impact. Product Composition for Biomimetics Anti-ageing Cream and Serum The cream and serum are designed to balance the skin microbiome, reduce age spots and discoloration, and provide ultra-hydration and ultra-brightening using biomimetics. The proposed unique products contain prebiotic, postbiotic, and probiotic ingredients in such a unique and optimized ratio that it balances and nourish the skin microbiome (trillions of microorganisms, like bacteria, fungi, and viruses found on the skin as invisible life forms are known as the skin microbiome). Our Optimized mix of natural prebiotics, probiotics, and postbiotics ingredients derived from natural processes such as fennentation and other organic sources that balance and nourish the skin microbiome. The unique antiaging cream contains prebiotic, postbiotic, and probiotic ingredients that balance and nourish the skin microbiome. Overall, our biomimetic anti-aging cream with prebiotic, postbiotic, and probiotic ingredients is a lab proven solution for those looking for a vegan and cruelty-free way to achieve youthful, healthy-looking skin. The unique biomimetic antiaging serum contains prebiotic, postbiotic, and probiotic ingredients that balance and nourish the skin microbiome. Overall, our biomimetic anti-aging serum with prebiotic, postbiotic, and probiotic ingredients is a lab proven solution for those looking for a vegan and cruelty-free way to achieve youthful, healthy-looking skin.

Claims

1. Beautification product with the skin nursing complex that comprises the aging resistance effect, be characterized in that this complex is that liposome by the mixture of the prebiotic, postbiotic and probiotic components, described cream and serum is dispersed in the gel network of water and gellant, this gel is included in the beautification product together with common cosmetic additive, adjuvant, active substance and composition thereof concentration with 0.1-1 lwt.%, and concentration data is based on the whole weight meter of preparation.

2. According to the beautification product of claim 1, w herein the prebiotic component that is Extract Mix of Oat Meal extract, Banna Extract and Asparagus in the formulation of anti-aging serum and cream is 0.6-1 wt.% with respect to the gross weight meter scope of complex.

3. According to the beautification product of claim 1, wherein the postbiotic components that is mixture of Alpha-Glucan Oligosaccharide, L. fermentum &L. delbrueckii, the fermented culture medium and metabolites in anti-aging cream and serum is l-2.5wt.% with respect to the gross weight meter scope of complex.

4. According to the beautification product of claim 1, wherein the probiotic components that is mixture of Lactobacillus plantarum (KPBBC7), Lactobacillus Arizonensis Ferment Filtrate, Bacillus Ferment &Saccharomyces Ferment Filtrate, Kombucha Tea, Lactobacillus Ferment Lysate, Bacillus Ferment &Saccharomyces Ferment Filtrate in anti-aging serum is 0.1-5 wt.% with respect to the gross weight meter scope of complex.

5. According to the beautification product of claim 1, wherein the probiotic components that is mixture of Lactobacillus plantarum (KPBBC7), Lactobacillus Fennent Lysate, Bacillus Ferment &Saccharomyces Ferment Filtrate, Lactobacillus Arizonensis Ferment Filtrate, Kombucha Tea in anti-aging cream is 0.5-5 wt.% with respect to the gross weight meter scope of complex.

6. According to the beautification product of claim 1, be characterized in that co-3 and / or m-6 fatty acids are included as further active substance.

7. Method for preparing the beautification product of the skin nursing complex that comprises the aging resistance effect is characterized in that:A) one or more oils and active component Centella asiatica (Gotu Kola) Flower / Leaf / Stem Extract, Rosa canina (Rosehip) Fruit Oil are to mix under 45-50 °C with water to form first mixture; Subsequently,B) be 1 by first mixture that a) makes, independent of additional mixture that makes with the adding speed adding that is no more than lOg / min under the OOOrpm, defined second mixture is to be made of phospholipid and optional further adjuvant and active substance;C) the 3rd mixture that forms therefore is to mixing under 600-800rpm, until making water-oil emulsion;D) combine water and gellant, and be no more than l,200rpm stirs down, till forming gel network; WithE) the 3rd mixture is poured in the gel of step d) in 15 40-mmiitc time under the 700rpm at minimum;F) according to e) gel that we get by with cosmetics adjuvant, active substance or its mixture at 25-35 °C be less than under the 700rpm and mix.

8. Use for averting or reducing the appearance of human skin aging of a beauty composition according to claim 1 to 6, wherein the averting or reducing of the appearance of human skin aging is with respect to a distinguishing^ selected from the group consisting of wrinkles, evening skin Colour, evening tone and combinations thereof.

9. Use of an Undaria pinnatifida Cell Culture Extract in the manufacture of a medicament for reducing skin irritation or sting caused by hydroxy acid.

10. Use according to claim 19, wherein the Undaria pinnatifida Cell Culture Extract is present in an amount of from 1% by weight of the medicament.

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