Cosmetic composition including microneedles impregnated with CICA extract as active ingredient and method for manufacturing the same

Abstract

To provide a cosmetic composition including microneedles impregnated with CICA extract as an active ingredient, and a method for manufacturing the same.SOLUTION: The present invention relates to a cosmetic composition including microneedles impregnated with CICA extract as an active ingredient, and a method for manufacturing the same. The composition imparts elasticity and regenerative power to the skin, and exhibits excellent effects in enhancing skin turnover, absorption level, absorption depth, and absorption rate, while also improving skin texture, particularly skin roughness, thereby making it suitable for use in the cosmetic compositions.SELECTED DRAWING: Figure 2

Description

[Technical field]

[0001] The present invention relates to a cosmetic composition containing microneedles impregnated with a deer extract as an active ingredient, and a method for producing the same. [Background technology]

[0002] The skin is the largest organ of the body, protecting various organs from external stimuli and fulfilling various physiological roles. However, as we age, the physiological and barrier functions of the skin decline due to various internal and external factors, making the skin more susceptible to damage even from minor external stimuli. Therefore, there is a need to develop cosmetics that can reduce skin damage caused by external stimuli.

[0003] As technology advances, the average lifespan of humans increases, and people become more interested in their skin and appearance, the market size for functional cosmetics continues to expand. Skin is the primary defense organ against various harmful substances and is an important immune organ. Factors that harm the skin are increasing, changes in dietary habits are slowing down the production and shedding of the stratum corneum, and the moisturizing factors and lipid content of the stratum corneum are decreasing due to the decline in the function of keratinocytes, resulting in an increase in the number of people with skin that cannot function normally, which is becoming a problem.

[0004] Such a decrease in the barrier function of the skin leads to cosmetic problems such as darkening of the skin, psoriasis, and excessive peeling of the keratin, as well as skin disease problems such as xeroderma, atopic dermatitis, etc. However, most of these problems can be prevented or alleviated by maintaining the moisture content of the skin, providing firmness and flexibility to the stratum corneum, and regulating the degree of contraction of the surrounding muscle tissue.

[0005] Even if the skin is healthy, it can become dry, flaky, and brittle due to various factors such as the effects of harsh external environments, wind, cold weather, sunlight, washing the face, shaving, various stresses, and natural skin aging that comes with age. Therefore, it is very important to properly regulate the moisture content of the skin's stratum corneum layer, the contractility of muscle cells, and protein activity.

[0006] In recent years, various types and forms of cosmetics are used. Cosmetics have beneficial functions for the skin, such as cleansing, moisturizing, blocking UV rays, improving wrinkles, whitening, and soothing, but they can also be a major cause of contact dermatitis. Contact dermatitis is dermatitis that occurs when the skin comes into contact with an external substance, and is classified into irritant contact dermatitis caused by irritant substances and allergic contact dermatitis that occurs in people who are sensitized to a specific antigen. Therefore, safety testing of cosmetics is important for the purpose of reducing such side effects.

[0007] Dermatitis caused by cosmetics occurs in less than 2% of users, of which allergic contact dermatitis accounts for 5-10% of side effects caused by cosmetics and 0.3-3% of patients who visit a dermatologist. Cosmetics are manufactured by mixing various ingredients, and since they come into direct contact with the human body and are used for long periods of time, the safety of not only the raw materials but also the cosmetics themselves must be ensured.

[0008] Conventional cosmetics contain glycerin, sorbitol, etc., but these have the problem of being sticky and causing discomfort when used. In addition, sodium pyrrolidone carboxylate, sodium lactate, etc., which are known as naturally derived additives, are highly electrolytic, making it difficult to maintain stability in the formulation. Conventional naturally derived raw materials either have poor effectiveness or cause many problems such as stability in the formulation and side effects due to skin irritation, so there is a need to develop cosmetic compositions that are not harmful to the human body and have excellent effects.

[0009] Under these circumstances, the present inventors have confirmed that a cosmetic composition containing as an active ingredient microneedles impregnated with a deer extract produced by the production method of the present invention provides elasticity and regenerative power to the skin, and has excellent effects of improving skin turnover, skin absorption amount, absorption depth, and absorption speed, as well as improving skin texture, i.e., skin roughness, and thereby completed the present invention. Summary of the Invention [Problem to be solved by the invention]

[0010] The present invention has been made in consideration of the above circumstances, and its object is to provide a cosmetic composition containing microneedles impregnated with deer extract as an active ingredient.

[0011] Another object of the present invention is to provide a method for producing a cosmetic composition, comprising the steps of: i) hydrolyzing a sponge after harvesting; ii) sieving the hydrolyzed sponge to separate microneedles; iii) drying the microneedles; and iv) stirring the dried microneedles and deer extract under reduced pressure to impregnate the deer extract into the inner tubes of the microneedles. [Means for solving the problem]

[0012] The present invention provides a cosmetic composition comprising microneedles impregnated with a deer extract as an active ingredient.

[0013] The deer extract of the present invention, also known as Centella asiatica extract, is a perennial plant belonging to the family Apiaceae. The stem spreads along the ground and has two degenerated scale-like leaves, each of which has a diameter of 2-5 cm. It produces crimson-purple flowers and flat, round fruits. It is known to have effects such as wound healing, antioxidant properties, promoting blood circulation, and improving vascular health.

[0014] The microneedle of the present invention is a spicule, which is a fine structure forming the skeleton of a marine sponge that lives in freshwater, and is a natural composite material consisting of calcium and silicate having a fine needle shape extracted from seaweed or coral.

[0015] The microneedle of the present invention may have a length of 200 to 300 μm and a diameter of 10 to 20 μm, specifically a length of 230 to 280 μm and a diameter of 12 to 18 μm, and more specifically a length of 250 μm and a diameter of 15 μm. If the size of the microneedle is less than the above range, it may penetrate into the skin in large quantities and cause inflammation, and if it exceeds the above range, the effect of improving skin regeneration and self-healing power is poor.

[0016] The pH of the microneedle of the present invention may be 7 to 8, specifically 7 to 7.5, and more specifically 7, but is not limited thereto.

[0017] The microneedles of the present invention are derived from a sponge, and the sponge may be Spongilla lacustris, Spongilla fragilis Leidy, or Ephydatia fluviatilis.

[0018] The content ratio of the deer extract to the microneedles of the present invention may be 0.5-2:98-99.5 parts by weight, specifically 0.5-1.5:98.5-99.5 parts by weight, and more specifically 1:99. If the content range of the deer extract to the microneedles is outside the above range, there is a risk that the deer extract will not be sufficiently contained in the microneedles.

[0019] The cosmetic composition of the present invention can have the effect of improving skin turnover. In one specific example of the present invention, it was confirmed that the general skin turnover cycle, which is 4 weeks or more, was shortened to 2 weeks when the cosmetic composition of the present invention was used, thereby promoting skin turnover.

[0020] The cosmetic composition of the present invention may have the effect of improving the skin's self-healing ability. In a specific embodiment of the present invention, the improvement in the skin's self-healing ability after use of the cosmetic composition of the present invention was measured, and it was confirmed that the measured value of the amount of skin erythema in the area where the cosmetic composition was used was reduced with a statistically significant difference 24 hours after exposure to ultraviolet rays compared to the area where the composition was not used.

[0021] The cosmetic composition of the present invention can have the effect of increasing the amount of skin absorption, the depth of absorption, and the rate of absorption. In a specific embodiment of the present invention, the amount of skin absorption, the depth of absorption, and the rate of absorption were measured after using the cosmetic composition of the present invention, and it was confirmed that the measured values ​​of the amount of skin absorption, the depth of absorption, and the rate of absorption were statistically significantly increased after using the cosmetic composition compared to before using the cosmetic composition.

[0022] The cosmetic composition of the present invention may have the effect of promoting improvement of skin roughness. In a specific embodiment of the present invention, the skin roughness, i.e., skin texture, was measured after use of the cosmetic composition of the present invention, and it was confirmed that the skin roughness analysis value was statistically significantly reduced immediately after use compared to before use of the cosmetic composition, thereby improving the skin texture.

[0023] The cosmetic composition of the present invention may be prepared in a dosage form selected from the group consisting of, but not limited to, a solution, an ointment for external use, a cream, a foam, a nutritious lotion, a softening lotion, a perfume, a pack, a softening lotion, a milky lotion, a makeup base, an essence, a soap, a liquid cleanser, a bath additive, a sunscreen cream, a sun oil, a suspension, an emulsion, a paste, a gel, a lotion, a powder, a surfactant-containing cleanser, an oil, a powder foundation, an emulsion foundation, a wax foundation, a patch, and a spray.

[0024] The cosmetic composition may further contain one or more cosmetically acceptable carriers that are commonly used in skin cosmetics, and may appropriately contain, as typical ingredients, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc., but is not limited thereto.

[0025] The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

[0026] When the dosage form of the present invention is an ointment, paste, cream or gel, the carrier component may be, but is not limited to, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, etc. These may be used alone or in combination of two or more.

[0027] When the dosage form of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. are used as carrier components, and especially in the case of a spray, a propellant such as hydrochlorofluorocarbon, propane / butane, or dimethyl ether can be additionally contained, but is not limited thereto. These can be used alone or in combination of two or more kinds.

[0028] When the dosage form of the present invention is a solution or emulsion, a solvent, solubilizer, or emulsifier is used as a carrier component. For example, water, glycerin, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, etc. are used, and in particular, cottonseed oil, peanut oil and corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol, or fatty acid esters of sorbitan are used, but are not limited thereto. These may be used alone or in combination of two or more.

[0029] When the dosage form of the present invention is a suspension, the carrier components include, but are not limited to, liquid diluents such as water, glycerin, ethanol, or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters, and polyoxyethylene sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant, etc. These may be used alone or in combination of two or more.

[0030] When the dosage form of the present invention is a soap, the carrier components include, but are not limited to, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. These may be used alone or in combination of two or more.

[0031] The present invention also provides a method for producing a cosmetic composition, comprising the steps of: i) hydrolyzing a sponge after harvesting it; ii) sieving the hydrolyzed sponge to separate microneedles; iii) drying the microneedles; and iv) stirring the dried microneedles and a deer extract under reduced pressure to impregnate the inner tubes of the microneedles with the deer extract.

[0032] In step i), the hydrolysis is carried out for 30 to 50 hours, but is not limited thereto.

[0033] In the step ii), the sieving may be performed with a mesh size of 50 to 200 mesh, specifically, 50 to 150 mesh, and more specifically, 100 mesh, but is not limited thereto.

[0034] In the step iii), the drying may be performed at 70 to 100° C. for 20 to 30 hours, more specifically, at 70 to 90° C. for 24 hours.

[0035] In step iv), the stirring under reduced pressure may be performed for 2 to 5 hours at -0.1 to 0.1 MPa, specifically for 2 to 4 hours at -0.07 to -0.05 MPa, and more specifically for 3 hours at -0.06 MPa. The deer extract can be sufficiently impregnated into the inner tube of the microneedle within the pressure and time range of the stirring under reduced pressure. Effect of the Invention

[0036] The present invention provides a cosmetic composition containing microneedles impregnated with a deer extract as an active ingredient, and a manufacturing method thereof. The composition can be usefully used as a cosmetic composition by providing elasticity and regenerative power to the skin, improving skin turnover, skin absorption amount, absorption depth, and absorption rate, and improving skin texture, i.e., skin roughness. [Brief description of the drawings]

[0037] [Figure 1] FIG. 2 is a graph showing the change in skin color as a result of measuring the skin turnover promoting effect after use of the cosmetic composition of the present invention. [Diagram 2] FIG. 2 is a graph showing the peeling rate of skin stratum corneum as a result of measuring the skin turnover promoting effect after use of the cosmetic composition of the present invention. [Diagram 3] FIG. 2 is a graph showing the measured amount of skin erythema, which was a result of measuring the effect of improving skin's self-healing ability after use of the cosmetic composition of the present invention. [Figure 4] FIG. 2 is a graph showing the results of measuring the amount of skin absorption after use of the cosmetic composition of the present invention. [Diagram 5] FIG. 2 is a graph showing the results of measuring the roughness of the skin, i.e., the texture of the skin, after use of the cosmetic composition of the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0038] The advantages and features of the present invention, as well as the methods for achieving them, will become apparent from the following detailed description of the embodiments in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, and may be embodied in various different forms. However, the embodiments are provided to complete the disclosure of the present invention and to allow those skilled in the art to fully understand the scope of the present invention, and the present invention is defined only by the scope of the claims.

[0039] The terms used in this specification are for the purpose of describing the embodiments and are not intended to limit the present invention. In this specification, the singular includes the plural unless otherwise specified. The terms "comprises" and / or "comprising" used in this specification do not exclude the presence or addition of one or more other components other than the components mentioned. The same reference numerals throughout this specification refer to the same components, and "and / or" includes each and every combination of one or more of the components mentioned. Even if "first", "second", etc. are used to describe various components, these components are not limited by these terms. These terms are used simply to distinguish one component from another component. Therefore, it goes without saying that the first component mentioned below may also be the second component within the technical spirit of the present invention.

[0040] Unless otherwise defined, all terms (including technical and scientific terms) used in this specification are used as meanings commonly understood by ordinary skilled artisans in the technical field to which the present invention belongs. Furthermore, terms defined in commonly used dictionaries are not interpreted ideally or excessively unless clearly and specifically defined.

[0041] Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes modifications of the technical ideas equivalent thereto.

[0042] Example 1. Preparation of cosmetic composition 1-1. Collection of the original Freshwater sponge from Ukraine, which lives in fresh water, was used as the raw material. The raw material was directly imported and used for purification.

[0043] The freshwater sponge is also called hydrized sponge or spicules, and the spicules are very small needles extracted from freshwater sponges that penetrate deep into the skin, stimulate the dermis, activate microcirculation in the skin, and help tighten skin pores.

[0044] 1-2. Hydrolysis of the original material The raw material was stirred for 30 minutes, and the temperature was gradually raised to 30-40°C, and the mixture was hydrolyzed with stirring for 48 hours.

[0045] 1-3. Primary screening and washing The raw material hydrolyzed in step 1-2 was sieved through a 100 mesh to separate the spicules, which were then washed five times.

[0046] 1-4. Drying and secondary screening The mixture was dried at 70 to 90° C. for 24 hours, and then sieved through a 200 mesh screen to remove foreign matter.

[0047] 1-5.Centella asiatica extract coating Dried spicules and Centella asiatica extract were put into a vacuum stirrer in a ratio of 1:1. The temperature of the vacuum stirrer was set to 80°C, and the vacuum pump was started to reduce the pressure inside the tank to -0.06 MPa while heating, and stirring was performed for 3 hours to dry and simultaneously impregnate the Centella asiatica extract into the inner tubes of the spicules. The cosmetic composition of the present invention contains Centella asiatica extract and spicules in a content ratio of 1:99 parts by weight.

[0048] 1-6. Packing and sterilization The container was primarily packed in Ziploc® bags, and then secondary packed in aluminum packs. After sterilization by gamma treatment, the cosmetic composition was finally prepared.

[0049] Experimental example 1. Improved skin turnover 1-1. Information on test subjects Information on the subjects who participated in the study is as shown in Table 1 below.

[0050] [Table 1]

[0051] 1-2. Test method A total of 22 test subjects were assigned test sites (2 x 2 cm) to separate areas where the cosmetic composition was used and areas where it was not used. Then, before dyeing, the skin was measured and photographed, and 8% DHA (Dihydroxyacetone) emulsion was applied at 2.0 mg / cm2 to each area. 2 The composition was applied to the area where the cosmetic composition of the present invention was used for two weeks, and the skin was measured and photographed before and after dyeing, and 1 day, 7 days, and 14 days after using the product.

[0052] 1-3.Evaluation method The improvement of skin turnover was evaluated by measuring skin color and taking UV photos of the skin. To measure skin color, a Chromameter CR 400 was used to measure the skin color (L value) of each area before and after dyeing, and after 1 day, 7 days, and 14 days of product use. Skin color was measured three times for each area, and the average value was substituted into the following formula to calculate the keratin peeling rate (%).

[0053] JPEG2024177006000003.jpg30163

[0054] The higher the exfoliation rate value of the dyed skin after using the product, the more effective it is at promoting skin turnover.

[0055] Additionally, UV photography of the skin was performed using a Janus 1 Mark II.

[0056] 1-4. Test results This test was conducted on 22 adult men and women aged 19 to 60 years old, and a total of 20 test subjects, excluding 2 who dropped out, used the test product, the cosmetic composition of the present invention, on the forearm for two weeks.

[0057] As a result of measuring the skin turnover promotion effect after using the test product, it was confirmed that the measured value of the keratin peeling rate of the test product application site was improved with a statistically significant difference after 14 days of product use compared to the test product non-use site (p<0.05). Skin color and skin keratin peeling rate are shown in Figures 1 and 2.

[0058] It was confirmed that the exfoliation rate value of the stained skin increased after use of the product (Table 2).

[0059] [Table 2]

[0060] This indicates that the cosmetic composition of the present invention is useful for improving skin turnover even with just two weeks of use. The general skin turnover cycle is four weeks or more, but when the cosmetic composition of the present invention is used, this is shortened to two weeks, suggesting that skin turnover is promoted.

[0061] Experimental example 2. Improved skin regeneration ability 2-1. Information on test subjects Information on the subjects who participated in the study is as shown in Table 1 above.

[0062] 2-2. Test method A total of 22 test subjects were assigned test sites (2 x 2 cm) to separate areas where the cosmetic composition was used and areas where it was not used. After the cosmetic composition was used on the test sites for self-healing ability for 2 weeks, the test sites were irradiated with ultraviolet light, and the skin was measured and photographed before and 24 hours after exposure to ultraviolet light.

[0063] 2-3.Evaluation method The improvement of skin's natural regeneration ability is measured at a wavelength of 290-320 nm, 44.22 mJ / cm 2 The skin barrier was temporarily damaged by irradiating the skin with ultraviolet light having a light intensity of 1000 nm using a Multiport. After that, general photography of the skin was performed using an Antera 3D, and the amount of skin erythema was measured using a Mexameter MX18. Skin erythema was evaluated by measuring the erythema index (EI) of the irradiated area before and 24 hours after UV irradiation. The average value of three measurements was used as the evaluation material for skin erythema, and the unit of the measured erythema index is AU (Arbitrary Unit). The measured value and the erythema index are proportional, so the lower the measured value, the more the occurrence of erythema is suppressed.

[0064] 2-4. Test results This test was conducted on 22 adult men and women aged 19 to 60 years old, and a total of 20 test subjects, excluding 2 who dropped out, used the test product, the cosmetic composition of the present invention, on the forearm for two weeks.

[0065] The improvement in skin's natural regeneration ability was measured after use of the test product, and it was confirmed that the amount of skin erythema measured in the areas where the test product was used was reduced 24 hours after exposure to UV rays with a statistically significant difference compared to areas where the test product was not used (p<0.05) (Figure 3).

[0066] The effect of the cosmetic composition of the present invention in improving skin self-healing ability was measured by measuring the change in the amount of skin erythema, and the results are shown in Table 3 below.

[0067] [Table 3]

[0068] This suggests that the cosmetic composition of the present invention has a significant effect on improving skin's natural regeneration ability after two weeks of use.

[0069] Experimental example 3. Improved skin absorption 3-1. Information on test subjects Information on the subjects who participated in the study is as shown in Table 4 below.

[0070] [Table 4]

[0071] 3-2. Measurement of skin absorption 3-2-1.Measurement method To measure the amount of skin absorption, the test subjects' forearms were divided into areas where no test product was used, areas where essence was used, and areas where the test product was used. The amount of skin absorption in each area was then measured using the gen2-SCA Performance before and 20 minutes after use of the test product.

[0072] The measured data was analyzed using Skin Tools 3.0, and the value measured once was used as the evaluation data for the amount of skin absorption. The unit of the measured amount of skin absorption is μg / cm. 3 The measured value is proportional to the degree of skin absorption, so a higher measured value for skin absorption means that more is absorbed into the skin.

[0073] 3-2-2.Measurement results The amount of skin absorption measured after using the test product and essence was confirmed to be statistically significantly higher after using the product than before (p<0.05) (Figure 4). In addition, the change in the total amount of skin absorption measured at the test product and essence application sites showed a statistically significant difference after using the product compared to the essence application sites (p<0.05).

[0074] 3-3. Measurement of skin absorption depth 3-3-1.Measurement method The skin absorption depth was measured by dividing the test subject's forearm into areas without the test product, areas with essence, and areas with the test product, and then using gen2-SCA Performance, the skin absorption amount at each area was measured before and 20 minutes after using the test product. The depth at which the skin absorption amount increased statistically significantly after using the product compared to before using the product was used as the evaluation data for the skin absorption depth. The measurement unit is μm, and the higher the measurement value, the deeper the absorption into the skin.

[0075] 3-3-2.Measurement results The skin absorption depth was measured after use of the test product and essence, and it was confirmed that the skin absorption depth increased statistically significantly to a depth of 25-30 μm 20 minutes after use of the product (p<0.05).

[0076] 3-4. Measurement of skin absorption rate 3-4-1.Measurement method The skin absorption rate was determined by dividing the skin absorption depth by the absorption time for each area. The calculated value is proportional to the improvement in the skin absorption rate, so the higher the calculated value, the faster the absorption.

[0077] 3-4-2.Measurement results Calculation of the skin absorption rate after use of the test product and essence showed a statistically significant increase in skin absorption depth to 25-30 μm and a skin absorption rate of 1.25-1.5 μm / min.

[0078] The results of improving skin absorption of the cosmetic composition of the present invention are summarized in Table 5 below.

[0079] [Table 5]

[0080] Experimental example 4. Improvement of skin roughness 4-1. Information on test subjects Information on the subjects who participated in the study is as shown in Table 4 above.

[0081] 4-2.Measurement method To measure skin roughness, Antera 3D was used to photograph the face and cheek area before and immediately after using the test product. A specific area of ​​the photograph was selected and analyzed for roughness and used as evaluation data. The unit of analyzed skin roughness is Ra. The analysis value and the degree of improvement in skin roughness (skin texture) are inversely proportional, so the lower the analysis value, the more the skin roughness (skin texture) is improved.

[0082] 4-3.Measurement results After using the test product, the roughness of the skin, i.e., the texture of the skin, was measured and it was confirmed that the analysis value of the roughness of the skin immediately after using the product was statistically significantly reduced compared to before using the product (p<0.05) (Figure 5).

[0083] The results of the skin roughness measurements are shown in Table 6 below.

[0084] [Table 6]

[0085] Since a lower skin roughness analysis value means a greater effect of improving skin texture, a rate of change expressed as a negative number indicates the degree of improvement in skin texture (roughness), suggesting that the cosmetic composition of the present invention has an excellent effect of improving skin roughness.

[0086] Experimental Example 5. Assessment of Adaptability The test subjects were instructed to fill out the adaptation diary that was distributed with the test product when using the test product according to the usage method and frequency of use. Test subjects who used the test product less than 80% of the 100% adaptation that should be used were excluded from the measurement data analysis.

[0087] The suitability of the product for use is shown in Table 7 below.

[0088] [Table 7]

[0089] Experimental Example 6. Evaluation of abnormal reactions For the safety of the test subjects, the occurrence of any abnormal reactions and the severity of symptoms were checked at each visit.

[0090] At each visit, the test subjects were checked for any abnormal reactions to the areas where the product was used, and no particular abnormal skin reactions were reported to the areas where the product was used during the period of use of the test product (Table 8).

[0091] [Table 8]

[0092] Experimental Example 7. Questionnaire evaluation of test subjects The test subjects completed a direct questionnaire evaluation on the effectiveness of the product in improving the skin after using the test product on a 5-point scale (5: very satisfied, 4: satisfied, 3: normal, 2: dissatisfied, 1: very dissatisfied) and on the preference scale (5: very much so, 4: so, 3: normal, 2: not so much, 1: not at all) and the number of test subjects who answered 3 points or more was expressed as a percentage (positive response rate).

[0093] As a result, it was confirmed that the effectiveness of the product in promoting skin turnover and improving the skin's natural regeneration ability was high with a score of 4 or more, and that the amount, depth and speed of absorption by the skin were improved, and that the roughness of the skin was also improved. It was also confirmed that the rate of positive responses to the preferences such as usability, fragrance and purchase intention was 100% (Tables 9 and 10).

[0094] [Table 9]

[0095] [Table 10]

[0096] Experimental Example 8. Safety Evaluation The test products were applied to the skin for 24 hours, and the skin reactions and mean scores of the skin reactions observed at the test sites were evaluated 30 minutes, 24 hours, and 48 hours after the application was removed.

[0097] The results of the skin patch test conducted on the test subjects are shown in Table 11 below.

[0098] [Table 11]

[0099] As a result, no irritation was observed with the test product 30 minutes, 24 hours, or 48 hours after removal. The average skin reaction score was 0.00, which was determined to be non-irritating according to the evaluation criteria.

[0100] Example 9. Statistical analysis of results To determine the significance of the change in skin measurements between the areas where the test product was used and the areas where it was not used, the statistical analysis program SPSS23.0 was used. After excluding data from subjects who dropped out during the study, statistical analysis was performed, and statistical significance was confirmed when p<0.05 with a 95% confidence interval.

[0101] For comparison between areas where the test product was used and areas where the test product was not used, satisfaction of normality was analyzed using the Independent T-test (parametric method), and non-satisfaction of normality was analyzed using the Mann-Whitney U test (nonparametric method).

[0102] To compare results before and after use of the test product, satisfaction of normality was analyzed using the Paired samples T-test (parametric method), and non-satisfaction of normality was analyzed using the Wilcoxon signed ranks test (nonparametric method).

[0103] This suggests that a cosmetic composition containing as an active ingredient microneedles impregnated with deer extract produced by the production method of the present invention provides elasticity and regenerative power to the skin, improves skin turnover, skin absorption amount, absorption depth, and absorption speed, and improves skin texture, i.e., skin roughness, and has excellent adaptability and safety effects.

[0104] Although the embodiments of the present invention have been described above, the present invention is not limited to the above embodiments, and can be manufactured in various different forms, and a person skilled in the art to which the present invention belongs can understand that the present invention can be embodied in other specific forms without changing the technical idea or essential features of the present invention. Therefore, the above-described embodiments should be understood as illustrative in all respects and not restrictive.

Claims

1. A cosmetic composition comprising microneedles impregnated with a deer extract as an active ingredient.

2. The cosmetic composition according to claim 1, wherein the content ratio of the deer extract to the microneedles is 0.5-2:98-99.5 parts by weight.

3. The cosmetic composition according to claim 1, wherein the microneedles have a length of 200 to 300 μm and a diameter of 10 to 20 μm.

4. The cosmetic composition according to claim 1, characterized in that the pH of the microneedles is 7 to 8.

5. The cosmetic composition according to claim 1 , wherein the microneedles are derived from a sponge.

6. 6. The cosmetic composition according to claim 5, wherein the sponge is Spongilla lacustris, Spongilla fragilis Leidy, or Ephydatia fluviatilis.

7. The cosmetic composition according to claim 1, which has an effect of improving skin turnover.

8. The cosmetic composition according to claim 1, which has an effect of promoting improvement of skin's natural regeneration ability.

9. The cosmetic composition according to claim 1, which has the effect of promoting improvement of skin roughness.

10. i) hydrolysis of the sponge after harvesting; ii) sieving the hydrolyzed sponge to separate the microneedles; iii) drying the microneedles; iv) stirring the dried microneedles and deer extract under reduced pressure to impregnate the deer extract into the inner tubes of the microneedles; A method for producing a cosmetic composition comprising the steps of:

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