Objective lens device and microscope observation device

Abstract

To provide an objective lens device capable of exhibiting excellent imaging performance even when there are significant changes in the immersion liquid and the thickness or optical properties of a container on which a sample is placed.SOLUTION: An objective lens device 1 includes: a first movable lens group A provided to be movable in the optical axis direction; a first correction device Ac that corrects axial chromatic aberration by moving the first movable lens group A in the optical axis direction; a second movable lens group B provided to be movable in the optical axis direction; and a second correction device Bc that moves the second movable lens group B in the optical axis direction.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a microscope objective lens and a microscope observation device. [Background technology]

[0002] Currently, cell aggregates obtained by culturing cells in a three-dimensional manner and research using cell aggregates are attracting attention. In recent years, cell aggregates are photographed using a microscope device, and the obtained microscope image data is screened for drug discovery using image analysis technology to evaluate drug efficacy (Patent Documents 1-2).

[0003] When photographing deep areas of the above-mentioned objects, an immersion objective lens is used to suppress the occurrence of spherical aberration due to refractive index mismatch, and the objects are often observed with an inverted microscope. Immersion objective lenses suitable for such applications are known (Patent Documents 3 to 6). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] JP 2022-78638 A [Patent Document 2] JP 2022-169853 A [Patent Document 3] JP 2023-32561 A [Patent Document 4] U.S. Patent No. 7,349,162 [Patent Document 5] Patent No. 6960487 [Patent Document 6] Japanese Patent Application Publication No. 5-196873 [Non-patent literature]

[0005] [Non-Patent Document 1] M. Bass, et al: Handbook of Optics vol.II, McGraw-Hill [Non-Patent Document 2] R. Kingslake: Lens Design Fundamentals, SPIE Summary of the Invention [Problem to be solved by the invention]

[0006] Meanwhile, in screening research, containers (called well plates, microplates, etc.; hereinafter referred to as well plates in this specification) in which multiple depressions (wells) for containing samples are formed in a matrix are sometimes used. By using such containers, it is possible to easily prepare a large number of samples under different conditions. Many such well plates have been disclosed, for example in Patent Document 1.

[0007] There are several challenges when observing samples contained in a well plate, as opposed to the conventional case where a sample is placed on a cover glass and observed.

[0008] First, most well plates are made of acrylic, polyethylene, polystyrene, etc., and products with a wide variety of shapes are available. In other words, there is variation in the shape (especially thickness) of well plates, and this variation leads to changes in optical performance.

[0009] Furthermore, polymer materials such as polystyrene have larger variations in optical properties and thickness compared to glass due to subtle differences in the composition of the materials and differences in the conditions of the injection molding process (Non-Patent Document 1).

[0010] These differences in thickness and optical properties mainly result in changes in spherical aberration and chromatic aberration (Non-Patent Document 2). Such changes in aberrations can lead to problems such as insufficient imaging performance being achieved and making it impossible to perform desired observations.

[0011] In addition, when using immersion objectives, there are cases where multiple immersion liquids are used separately. Using different immersion liquids changes the refractive index and Abbe number, which in turn changes the spherical aberration and chromatic aberration.

[0012] With the above issues in mind, existing immersion objective lenses will be examined in detail below.

[0013] Patent Document 3 discloses an objective lens that corrects spherical aberration and chromatic aberration by moving some lens groups in the optical axis direction when an immersion liquid in which at least one of the refractive index and the Abbe number differs by 5% or more is changed. However, since this method simultaneously corrects spherical aberration and chromatic aberration, there is a problem that precise correction cannot be performed when an immersion liquid in which the refractive index is the same but the Abbe number is different is changed. This situation corresponds to, for example, a case where immersion liquid A and immersion liquid E are used selectively among the immersion liquids listed in Patent Document 3, but a correction method in that case is not disclosed.

[0014] Patent Document 4 discloses an objective lens that can select a state in which aberrations are appropriately corrected by appropriately moving multiple lens groups in response to changes in cover glass thickness, temperature, and immersion liquid. However, this method moves each lens group simultaneously, and has the problem that although it can handle several types of patterns assumed at the time of design, it cannot handle other cases.

[0015] Patent Document 5 discloses an objective lens that corrects spherical aberration and chromatic aberration by inserting and removing an appropriate parallel plane plate according to the immersion liquid. However, this method allows discrete adjustment for a certain immersion liquid, but cannot be applied to immersion liquids with unknown optical properties that were not anticipated at the time of design. In addition, no consideration is given to changes in the optical properties of the container in which the sample is placed. Furthermore, there is a problem that it is necessary to secure space on the sample side to hold the parallel plane plate, which leads to an increase in the size of the objective lens.

[0016] Patent Document 6 discloses an objective lens that corrects aberrations by inserting and removing a correction lens depending on whether a cover glass is present or not. However, this method has the problem that, although it can handle the change between the two states of whether or not a cover glass is present, it cannot handle the case where a cover glass is present and has a different thickness.

[0017] The present invention has been made in consideration of the above circumstances, and aims to provide an objective lens device that can exhibit good imaging performance even when there are large changes in the immersion liquid and in the thickness and optical characteristics of the container in which the sample is placed. [Means for solving the problem]

[0018] In order to solve the above problem, one aspect of the present invention is an objective lens device comprising a first movable lens group (A) movable in the optical axis direction, a first correction device (Ac) that corrects axial chromatic aberration by moving the first movable lens group in the optical axis direction, a second movable lens group (B) that can be moved in the optical axis direction, and a second correction device (Bc) that corrects spherical aberration by moving the second movable lens group in the optical axis direction.

[0019] According to this aspect, different aberrations can be corrected by each of the first and second correction devices, so that the imaging performance of the objective lens device can be improved even when there are large changes in the immersion liquid and in the thickness and optical characteristics of the container in which the sample is placed.

[0020] In the above aspect, the first movable lens group (A) may be composed of a cemented lens formed by cementing a plano-convex lens (A1) and a plano-concave lens (A2) together.

[0021] According to this embodiment, the first movable lens group can be realized with a simple lens configuration.

[0022] In the above aspect, it is preferable that the plano-convex lens (A1) and the plano-concave lens (A2) satisfy the following conditional expressions (1) and (2). |n1-n2|<0.05 (1) |v1-v2|>10 ···(2) however, n1: the refractive index of the plano-convex lens (A1) at the reference wavelength n2: the refractive index of the plano-concave lens (A2) at the reference wavelength v1: Abbe number of the plano-convex lens (A1) v2: the Abbe number of the plano-concave lens (A2).

[0023] According to this embodiment, it is possible to improve the imaging performance of the objective lens unit when there is a large change in the immersion liquid and in the thickness and optical characteristics of the container in which the sample is placed.

[0024] In order to solve the above problem, another aspect of the present invention is a microscope observation device (100) comprising an objective lens device (1) configured to include the first movable lens group (A), the first correction device (Ac), the second movable lens group (B), and the second correction device (Bc), a control device (20) that controls the first correction device (Ac) and the second correction device (Bc) of the objective lens device, an imaging lens (2) that forms an image of the objective lens device, and an image acquisition device (3) that acquires the image formed by the imaging lens, wherein the control device calculates a correction amount from image data acquired by the image acquisition device, and controls the first correction device and the second correction device based on the calculated correction amount.

[0025] According to this aspect, the axial chromatic aberration can be corrected by the first corrector, and the spherical aberration can be corrected by the second corrector, so that the imaging performance of the objective lens device can be improved even when there are large changes in the immersion liquid and in the thickness and optical characteristics of the container in which the sample is placed. Effect of the Invention

[0026] According to the above aspects, it is possible to realize an objective lens device and a microscope observation device that can individually correct spherical aberration and chromatic aberration and obtain good imaging performance even when the thickness or optical characteristics of the container in which the sample is placed change, or the immersion liquid changes. [Brief description of the drawings]

[0027] [Figure 1] FIG. 1 is a diagram showing the configuration of an objective lens device according to an embodiment of the present invention. [Diagram 2] FIG. 4 is a diagram showing spherical aberration of a reference wavelength in a normal state and in a state in which a movable lens group is moved in the objective lens device according to the first embodiment; [Diagram 3] FIG. 4 is a diagram showing a change in axial chromatic aberration when a movable lens group is moved from a normal state in the objective lens device according to the first embodiment. [Figure 4] Optical path diagram of Example 1 [Diagram 5] Longitudinal aberration diagram of the first state of the first embodiment [Figure 6] Longitudinal aberration diagram of the second state of the first embodiment [Figure 7] Longitudinal aberration diagram of the third state of the first embodiment [Figure 8] Transverse aberration diagram of the first state of the first embodiment [Figure 9] Transverse aberration diagram of the second state of the first embodiment [Figure 10] Transverse aberration diagram of the third state of the first embodiment [Figure 11] Optical path diagram of Example 2 [Figure 12] Longitudinal aberration diagram of the first state of the second embodiment [Figure 13] Longitudinal aberration diagram of the second state of the second embodiment [Figure 14] Longitudinal aberration diagram of the third state of the second embodiment [Figure 15] Transverse aberration diagram of the first state of the second embodiment [Figure 16] Transverse aberration diagram of the second state of the second embodiment [Figure 17] Transverse aberration diagram of the third state of the second embodiment [Figure 18] Optical path diagram of Example 3 [Figure 19]Longitudinal aberration diagram of the first state of the third embodiment [Figure 20] Longitudinal aberration diagram of the second state of the third embodiment [Figure 21] Longitudinal aberration diagram of the third state of the third embodiment [Figure 22] Transverse aberration diagram of the first state of the third embodiment [Figure 23] Transverse aberration diagram of the second state of the third embodiment [Figure 24] Transverse aberration diagram of the third state of the third embodiment [Diagram 25] Schematic diagram of a microscope observation device according to Example 4 DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0028] First, an objective lens device 1 according to one embodiment of the present invention will be described.

[0029] As shown in Fig. 1, the objective lens device 1 includes an optical system 10 consisting of a plurality of lenses that constitute an objective lens, and a lens barrel 11 that holds the optical system 10. The objective lens device 1 includes a first movable lens group A that is movable in the optical axis direction in the optical system 10. The objective lens device 1 can correct axial chromatic aberration by moving the first movable lens group A in the optical axis direction using a first corrector Ac. The first corrector Ac is a drive device that moves the first movable lens group A relative to the lens barrel 11.

[0030] Furthermore, the objective lens device 1 includes a second movable lens group B that is movable in the optical axis direction in the optical system 10. The objective lens device 1 can correct spherical aberration by moving the second movable lens group B in the optical axis direction using a second corrector Bc. The second corrector Bc is a drive device that moves the second movable lens group B relative to the lens barrel 11.

[0031] In this way, the objective lens device 1 includes two movable lens groups A and B, the first movable lens group A for correcting axial chromatic aberration and the second movable lens group B for correcting spherical aberration, and can correct axial chromatic aberration and spherical aberration separately by moving each of them separately. This allows flexible aberration correction to be achieved.

[0032] The first corrector Ac and the second corrector Bc are embodied, for example, by correction collars. Alternatively, they may be embodied by a method in which each of the movable lens groups A and B is moved by an electric actuator. When an electric actuator is used, flexible control becomes possible by electrically connecting it to a microscope device equipped with a computer.

[0033] The first movable lens group A is composed of a cemented lens in which a plano-convex lens A1 and a plano-concave lens A2 are cemented together. In this case, the plano-convex lens A1 and the plano-concave lens A2 satisfy the following conditional expressions (1) and (2). |n1-n2|<0.05 (1) |v1-v2|>10 ···(2) where n1 is the refractive index of the plano-convex lens A1 at the reference wavelength, n2 is the refractive index of the plano-concave lens A2 at the reference wavelength, v1 is the Abbe number of the plano-convex lens A1, and v2 is the Abbe number of the plano-concave lens A2.

[0034] It is more desirable that the plano-convex lens A1 and the plano-concave lens A2 satisfy the following conditional expressions (3) and (4). |n1-n2|<0.01 (3) |v1-v2|>20 ···(4)

[0035] The above formula means that two glass materials with nearly equal refractive indices at the reference wavelength and different Abbe numbers are cemented together. By using this type of configuration, it becomes possible to selectively change the axial chromatic aberration.

[0036] Now, consider moving this first movable lens group A in the optical axis direction to change the height of the light ray incident on first movable lens group A. In this case, since first movable lens group A can be regarded as a plane-parallel plate for the reference wavelength, the change in the light ray height does not affect the aberration. However, since first movable lens group A has power for wavelengths other than the reference wavelength, the change in the light ray height changes the axial chromatic aberration.

[0037] The aberration correction method of the present invention will be described using a specific example.

[0038] 2 shows the spherical aberration of the reference wavelength superimposed in the normal state and in the state (moving state) in which the first movable lens group A is moved in the objective lens device 1 according to Example 1 described later. As can be seen from FIG. 2, even when the first movable lens group A is moved, the spherical aberration of the reference wavelength hardly changes.

[0039] Fig. 3 shows the effect on chromatic aberration when the first movable lens group A is moved from the normal state in the objective lens device 1 according to Example 1, specifically, the amount of change in axial chromatic aberration (focus shift). As can be seen from Fig. 3, the axial chromatic aberration changes significantly when the first movable lens group A is moved.

[0040] As shown in FIGS. 2 and 3, by moving the first movable lens group A, it is possible to change the axial chromatic aberration without causing a significant change in the spherical aberration of the reference wavelength.

[0041] By providing the second movable lens group B with a spherical aberration correction function, it becomes possible to effectively correct both spherical aberration and longitudinal chromatic aberration.

[0042] Next, an observation method according to another aspect of the present invention will be described.

[0043] The observation method is embodied by an observation device exemplified in Fig. 18. Details will be described in the third embodiment, but the observation device is a microscope observation device 100, which includes a microscope body 15 having at least one objective lens device 1, and a control device 20 that controls the microscope body 15. The microscope body 15 further includes an imaging lens 2 and an image acquisition device 3. The objective lens device 1 includes a first correction device Ac (Fig. 1) that corrects axial chromatic aberration, and a second correction device Bc (Fig. 1) that corrects spherical aberration.

[0044] The control device 20 includes an objective lens corrector control device 21, a microscope control device 22, and a calculation terminal 23. The calculation terminal 23 calculates the amount of residual aberration based on image information acquired by the image acquisition device 3. At this time, the calculation terminal 23 may use a resolution chart or the like as an observation target. The calculation terminal 23 calculates the amount of image point shift and the amount of residual aberration from the image, and based on this, the objective lens corrector control device 21 calculates the amount of movement of each of the movable lens groups A and B. Based on this, the control device 20 drives the first corrector Ac and the second corrector Bc via the microscope control device 22, thereby achieving a state in which good imaging performance is exhibited.

[0045] Thus, according to the present invention, it is possible to realize an objective lens device 1 and a microscope observation device 100 that can individually correct spherical aberration and chromatic aberration and obtain good imaging performance even when the thickness or optical characteristics of the container 6 in which the sample is placed change, or the immersion liquid changes.

[0046] Several embodiments of the objective lens device 1 of the present invention will be described below. Figs. 4 to 10 and Tables 1 to 4 show Example 1, Figs. 11 to 17 and Tables 5 to 8 show Example 2, Figs. 18 to 24 and Tables 9 to 12 show Example 3, and Fig. 25 shows Example 4.

[0047] Tables 1 and 5 show lens data for the objective lens device 1. Tables 2 and 6 show basic data such as the focal length of the objective lens device 1. Unless otherwise specified, specifications are values ​​at the reference wavelength. Tables 3 and 7 show immersion liquid data used for the objective lens device 1. The "immersion liquid data" shows the refractive index and Abbe number of each immersion liquid. Tables 4 and 8 show variable distance data for the objective lens device 1. The "variable distance data" shows the variable distance values ​​associated with changes in the first to third states.

[0048] In addition, for all the values ​​of the following specifications, the focal length, radius of curvature, lens surface spacing, and other lengths are expressed in millimeters (mm) unless otherwise specified. However, since the optical system can obtain the same optical performance with proportional magnification and proportional reduction, this is not limited to this. EXAMPLES

[0049] Fig. 4 shows an optical path diagram of the objective lens device 1 of Example 1. Figs. 5 to 7 show longitudinal aberration diagrams of the objective lens device 1 in the first to third states, and Figs. 8 to 10 show lateral aberration diagrams of the objective lens device 1 in the first to third states.

[0050] The objective lens device 1 according to the first embodiment is composed of a first fixed lens group FA, a first movable lens group A, a second fixed lens group FB, a second movable lens group B, and a third fixed lens group FC. The first fixed lens group FA is composed of a plurality of lens components including a cemented lens (a first lens component L1 (cemented lens) and a second lens component L2 (single lens) both made of concave meniscus lenses). Here, the lens component means one lens made of a cemented lens or a single lens. The first movable lens group A is composed of a cemented lens (a third lens component L3) in which a plano-convex lens A1 and a plano-concave lens A2 are cemented together. The second fixed lens group FB is composed of a cemented lens (a fourth lens component L4 made of a convex meniscus lens). The second movable lens group B is composed of two cemented lenses (a fifth lens component L5 and a sixth lens component L6) each of which forms a biconvex lens. The third fixed lens group FC is composed of a plurality of lenses including a cemented lens (a seventh lens component L7 formed of a biconvex lens, an eighth lens component L8 formed of a convex meniscus lens, and a ninth lens component L9.

[0051] The second movable lens group B is movable in the direction of the optical axis. Making the lens group with a high light ray height movable is effective in correcting spherical aberration.

[0052] Moreover, the light beam emerging from the second movable lens group B is approximately afocal. With this configuration, it is possible to suppress a change in axial chromatic aberration when the second movable lens group B is moved.

[0053] The first corrector Ac moves the first movable lens group A in the optical axis direction, and the second corrector Bc moves the second movable lens group B in the optical axis direction.

[0054] The object to be observed is placed in a polystyrene well plate, although only the bottom of the well is shown in the optical path diagram in Figure 4.

[0055] The gap between the well plate and the objective lens arrangement 1 is filled with an immersion liquid.

[0056] The first state is a reference state, in which a well plate of a given thickness and a given immersion liquid are used. The reference wavelength is the d-line. The second state is a state in which an immersion liquid different from that of the first state is used. Furthermore, the third state is a state in which the thickness of the well plate is different from that of the second state.

[0057] The lens data for Example 1 are shown below. All glass materials beginning with "S-" are Ohara glass names. POLYSTYR is polystyrene. [Table 1] [Table 2] [Table 3] [Table 4]

[0058] Longitudinal aberration diagrams of the objective lens device 1 of Example 1 in the first to third states are as shown in Figures 5 to 7. Moreover, lateral aberration diagrams of the objective lens device 1 of Example 1 in the first to third states are as shown in Figures 8 to 10. EXAMPLES

[0059] Fig. 11 shows an optical path diagram of the objective lens device 1 of Example 2. Figs. 12 to 14 show longitudinal aberration diagrams of the objective lens device 1 in the first to third states, and Figs. 15 to 17 show lateral aberration diagrams of the objective lens device 1 in the first to third states.

[0060] The objective lens device 1 according to the second embodiment is composed of a second movable lens group B and a first fixed lens group FA. The first movable lens group A is provided in the second movable lens group B. As the second movable lens group B moves in the optical axis direction, the first movable lens group A also moves integrally therewith.

[0061] The second movable lens group B is composed of four cemented lenses (an eleventh lens component L11 consisting of a concave meniscus lens, a twelfth lens component L12 consisting of a flat lens, a thirteenth lens component L13 consisting of a concave meniscus lens, and a fourteenth lens component L14 consisting of a plano-convex lens). The first movable lens group A is composed of a cemented lens (a twelfth lens component L12) in which a plano-convex lens A1 and a plano-concave lens A2 are cemented together. The first fixed lens group FA is composed of a plurality of lenses including a cemented lens (a fifteenth lens component L15 consisting of a biconvex lens, and sixteenth lens component L16, seventeenth lens component L17, and eighteenth lens component L18, all of which are convex meniscus lenses).

[0062] When the first movable lens group A is moved in the optical axis direction to correct axial chromatic aberration, the second movable lens group B is fixed.

[0063] The first corrector Ac moves the first movable lens group A in the optical axis direction, and the second corrector Bc moves the second movable lens group B in the optical axis direction.

[0064] The first movable lens group A is composed of a cemented lens in which a plano-convex lens A1 and a plano-concave lens A2 are cemented together.

[0065] The object to be observed is placed in a polystyrene well plate, although only the bottom of the well is shown in the optical path diagram in Figure 11.

[0066] The gap between the well plate and the objective lens arrangement 1 is filled with an immersion liquid.

[0067] The first state is a reference state, in which a well plate of a given thickness and a given immersion liquid are used. The reference wavelength is the d-line. The second state is a state in which an immersion liquid different from that of the first state is used. Furthermore, the third state is a state in which the thickness of the well plate is different from that of the second state.

[0068] The lens data for Example 2 are shown below. All glass materials beginning with "S-" are Ohara glass names. POLYSTYR is polystyrene. [Table 5] [Table 6] [Table 7] [Table 8]

[0069] 12 to 14 show longitudinal aberration diagrams of the objective lens device 1 of Example 2 in the first to third states, and FIGS. 15 to 17 show lateral aberration diagrams of the objective lens device 1 of Example 2 in the first to third states. EXAMPLES

[0070] Fig. 18 shows an optical path diagram of the objective lens device 1 of Example 3. Figs. 19 to 21 show longitudinal aberration diagrams of the objective lens device 1 in the first to third states, and Figs. 22 to 24 show lateral aberration diagrams of the objective lens device 1 in the first to third states.

[0071] The objective lens device 1 according to the third embodiment is composed of a first fixed lens group FA, a second movable lens group B, a first movable lens group A, and a second fixed lens group FB. The first fixed lens group FA is composed of a 21st lens component L21 composed of a cemented lens. The second movable lens group B is composed of a 22nd lens component L22 composed of a meniscus lens having the same curvature on both sides. The first movable lens group A is composed of a cemented lens (a 23rd lens component L23) in which a plano-convex lens A1 and a plano-concave lens A2 are cemented together. The second fixed lens group FB is composed of a plurality of lenses including a cemented lens (a 24th lens component L24 composed of a cemented lens, a 25th lens component L25 composed of a cemented lens, a 26th lens component L26 composed of a cemented lens, a 27th lens component L27 composed of a biconvex lens, a 28th lens component L28 composed of a convex meniscus lens, and a 29th lens component L29 composed of a parallel plane plate).

[0072] When the first movable lens group A is moved in the optical axis direction to correct axial chromatic aberration, the second movable lens group B is fixed.

[0073] The first corrector Ac moves the first movable lens group A in the optical axis direction, and the second corrector Bc moves the second movable lens group B in the optical axis direction.

[0074] The first movable lens group A is composed of a cemented lens in which a plano-convex lens A1 and a plano-concave lens A2 are cemented together.

[0075] The second movable lens group B is composed of a meniscus lens having the same curvature on both sides, which effectively eliminates the power of the second movable lens group B, making it possible to selectively change the spherical aberration without significantly affecting the axial chromatic aberration.

[0076] The object to be observed is placed in a polystyrene well plate, although only the bottom of the well is shown in the optical path diagram in Figure 18.

[0077] The gap between the well plate and the objective lens arrangement 1 is filled with an immersion liquid.

[0078] The first state is a reference state, in which a well plate of a given thickness and a given immersion liquid are used. The reference wavelength is the d-line. The second state is a state in which an immersion liquid different from that of the first state is used. Furthermore, the third state is a state in which the thickness of the well plate is different from that of the second state.

[0079] The lens data for Example 2 are shown below. All glass materials beginning with "S-" are Ohara glass names. POLYSTYR is polystyrene. [Table 9] [Table 10] [Table 11] [Table 12] EXAMPLES

[0080] 18 is a schematic diagram showing the concept of a microscope observation apparatus 100 according to the present invention. The microscope observation apparatus 100 includes at least an objective lens device 1, an objective lens correction device control device 21 that controls the objective lens correction device, and an image acquisition device 3. The objective lens device 1 also includes a first correction device Ac that corrects axial chromatic aberration, and a second correction device Bc that corrects spherical aberration.

[0081] A sample S observed by the microscope observation device 100 is, for example, cultured cells contained in a container 6 such as a well plate.

[0082] The transmitted illumination system 5 illuminates the sample S placed on the stage 7 from above the stage 7.

[0083] As shown in the figure, a plurality of objective lens devices 1 are provided below the stage 7 so as to be switchable by a revolver 8. The objective lens device 1 forms an optical image of the sample S in combination with an imaging lens 2.

[0084] An optical image of the sample S is acquired as an image by the image acquisition device 3.

[0085] The control device 20 preferably comprises a computing terminal 23 (eg a computer) on which the images captured by the image capture device 3 are output.

[0086] The first corrector Ac and the second corrector Bc provided in the objective lens device 1 are controlled by the calculation terminal 23 through the microscope control device 22. Based on the image data acquired by the image acquisition device 3, a state in which good imaging performance is exhibited can be obtained with an appropriate amount of correction. [Explanation of symbols]

[0087] 1: Objective lens device 2: Imaging lens 3: Image acquisition device 5: Transillumination system 6: Container 7: Stage 8: Nosepiece 15: Microscope body 20: Control device 21: Objective lens correction device control device 22: Microscope control device 23: Calculation terminal 100: Microscope observation device A: First movable lens group A1: Plano-convex lens A2: Plano-concave lens Ac: 1st correction device B: Movable lens group Bc:Second correction device FA: First fixed lens group FB: Second fixed lens group FC: Third fixed lens group S: Sample

Claims

1. An objective lens device, A first movable lens group that is movable in the optical axis direction; a first correction device that moves the first movable lens group in an optical axis direction; A second movable lens group provided so as to be movable in the optical axis direction; and a second correction device that moves the second movable lens group in the optical axis direction.

2. 2. The objective lens device according to claim 1, wherein the first movable lens group is composed of a cemented lens formed by cementing a plano-convex lens and a plano-concave lens together.

3. 3. The objective lens device according to claim 2, wherein the plano-convex lens and the plano-concave lens satisfy the following conditional expressions (1) and (2): |n １ -n ２ |<0.05 ・・・(1) |v １ -v ２ |>10 ・・・(2) however, n １ : the refractive index of the plano-convex lens at a reference wavelength n ２ : the refractive index of the plano-concave lens at a reference wavelength v １ : Abbe number of the plano-convex lens v ２ : the Abbe number of the plano-concave lens.

4. A microscope observation apparatus, comprising: An objective lens device according to any one of claims 1 to 3, a control device for controlling the first correction device and the second correction device of the objective lens device; an imaging lens for forming an image of the objective lens device; an image capture device for capturing an image formed by the imaging lens; A microscope observation apparatus, wherein the control device calculates a correction amount from image data acquired by the image acquisition device, and controls the first correction device and the second correction device based on the calculated correction amount.

Images