Use of a composition of alcohol and a cooling agent in regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality

Abstract

Use of a composition of alcohol and a cooling agent in regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, fighting tumors, and improving immunity and sleep quality in the fields of medicine and health care products and food. The components of the composition include alcohol and a cooling agent. In addition, the composition includes an organic acid. In the composition, the alcohol content is 1-99% by volume, and the cooling agent content is 0.05-500 ppm. The composition can regulate sexual function, protect the cardiovascular and cerebrovascular systems, promote liver cell regeneration, fight tumors, and improve immunity and sleep quality within a short period of time.

Description

[Technical field]

[0001] The present invention belongs to the fields of medicine, health care and food, specifically, to the use of a composition of alcohol and a cooling agent in regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality. [Background technology]

[0002] Most people are busy working under heavy pressure, often drinking heavily in social situations and lacking exercise. With the increasing pressure of life and work, the human body tends to enter a state of long-term sympathetic hypertension. The persistent sympathetic hyperexcitation leads to many diseases, such as autoimmune depression, sexual dysfunction and insufficient sleep or insomnia.

[0003] Long-term excessive drinking causes cardiovascular and cerebrovascular diseases, alcoholic liver disease (ALD), and tumors. In 2021, the International Agency for Research on Cancer (IARC), a branch of the WHO, published the Lancet Oncology report on the global burden of alcohol-related cancers. The report shows that in 2020, approximately 740,000 new cancers were related to alcohol consumption worldwide, including 76.7% of men and 23.3% of women. There are more than 270 million patients in China with cardiovascular and cerebrovascular diseases. Alcoholic liver disease (ALD) is a common clinical type of liver damage. The latest epidemiological surveys have found that ALD remains one of the common diseases that seriously endanger public health worldwide and is increasing year by year. More than 90% of alcoholics are prone to fatty liver, 10%-35% develop alcoholic hepatitis, and about 8%-20% of chronic alcoholics progress to cirrhosis or even develop liver cancer. In China, nearly 3 million people die from cardiovascular and cerebrovascular diseases every year, accounting for 51% of the total annual causes of death. However, 75% of survivors lose the ability to work to various degrees, and 40% suffer from severe disabilities.

[0004] Non-Patent Document 1 surveyed 100,000 people across all provinces in China. The survey results showed that 83.81% of the respondents were often bothered by sleep problems. The survey showed that sleep problems in China are increasing year by year, sleep problems are gradually turning into social problems, and people with sleep problems often suffer from autoimmune depression, sexual dysfunction and other symptoms.

[0005] Most of the people who have the above health problems, such as immunosuppression, sexual dysfunction and insufficient sleep, cannot detect the actual disease and cannot be effectively treated by drugs for a long time.Generally, they are treated through diet or by administering nutritional supplements, but these methods may not be effective in short-term alleviation of immunosuppression, sexual dysfunction, insufficient sleep and other health problems.In addition, the treatment through diet or nutritional supplements takes a long time and has a slow effect, so it is difficult for patients to continue such treatment. [Prior art documents] [Non-patent literature]

[0006] [Non-Patent Document 1] 2018 China Sleep Quality Survey Report released by the Sleep and Breathing Center of Beijing Chaoyang Hospital Summary of the Invention [Means for solving the problem]

[0007] The present invention provides the use of a composition of alcohol and a cooling agent in regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality.

[0008] Furthermore, the alcohol content in the composition is 1% by volume to 99% by volume, and the cooling sensation agent therein is 0.05 ppm to 500 ppm.

[0009] Preferably, the alcohol in the composition is 30% to 80% by volume, and the cooling agent therein is 10 ppm to 200 ppm.

[0010] More preferably, the alcohol in the composition is 30% by volume to 60% by volume, and the cooling agent therein is 10 ppm to 100 ppm.

[0011] Furthermore, the cooling agent is a substance capable of activating the thermoreceptor TRPM8 receptor. The cooling agent includes at least one of menthyl acetate, L-menthyl lactate, monomethyl succinate, L-monomethyl glutarate, L-menthol ethylene glycol carbonate, L-menthol 1-(or 2-)-propylene glycol carbonate, 3-L-menthoxypropane-1,2-diol, 3-L-menthoxy-2-methylpropane-1,2-diol, N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide, N,2,3-trimethyl-2-isopropylbutamide, menthol, or menthone.

[0012] Additionally, the composition includes an organic acid.

[0013] Furthermore, the organic acid content is 150 ppm to 30,000 ppm.

[0014] Furthermore, the organic acid is 1,000 ppm to 10,000 ppm.

[0015] Furthermore, the organic acid content is 1,000 ppm to 6,000 ppm.

[0016] Further, the organic acid is selected from at least one of formic acid, acetic acid, propionic acid, lactic acid, butanoic acid, succinic acid, pentanoic acid, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, citric acid, malic acid, tartaric acid, oxalic acid, oleic acid, linoleic acid, palmitic acid, or lauric acid.

[0017] The beneficial effect of the present invention is that a cooling agent is added to a base liquor of a certain alcoholic concentration, or natural plants containing menthol (peppermint and spearmint) are added to participate in all the fermentation stages of various solutions to obtain a cooling agent, and then the cooling agent and alcohol are controlled by blending or refining to prepare a composition with the ability of regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immune and sleep quality. The composition of the present invention can play the role of regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immune and sleep quality within 5 to 7 days by activating the parasympathetic signaling pathway, thereby largely solving the problem that common dietary therapy and dietary supplements cannot improve sexual function, immune and sleep quality within a short period of time, and enabling the protection, recovery and repair of the organism. The additional organic acid in the base liquor containing the cooling agent has a good effect. The composition of the present invention can be used in the fields of medicine, food and health care products. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0018] Detailed Description of the Invention The term "liquor" refers to a distilled, fermented, alcoholic beverage or mixed alcoholic beverage.

[0019] The human body is a complex system, and maintains the balance of bodily functions through various adjustments, not a single adjustment. The present invention provides the use of a composition of alcohol and a cooling agent in regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality. The alcohol in the composition of the present invention is 1% by volume to 99% by volume, and the cooling agent therein is 0.05 ppm to 500 ppm. Preferably, the alcohol in the composition is 30% by volume to 80% by volume, and the cooling agent therein is 10 ppm to 200 ppm. More preferably, the alcohol in the composition is 30% by volume to 60% by volume, and the cooling agent therein is 10 ppm to 100 ppm.

[0020] The alcohol of the present invention is selected from at least one of distilled alcohol, fermented alcohol, and edible alcohol.

[0021] The spirit is selected from at least one of sake, brandy, whiskey, vodka, rum, gin, tequila, or fruit spirits.

[0022] The fermented alcoholic beverage is selected from at least one of Chartreuse, beer, sake, fruit wine, refined rice liqueur, and milk liqueur.

[0023] The cooling agent is a substance capable of activating the thermoreceptor TRPM8 receptor, and includes at least one of menthyl acetate, L-menthyl lactate, monomethyl succinate, L-monomethyl glutarate, L-menthol ethylene glycol carbonate, L-menthol 1-(or 2-)-propylene glycol carbonate, 3-L-menthoxypropane-1,2-diol, 3-L-menthoxy-2-methylpropane-1,2-diol, N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide, N,2,3-trimethyl-2-isopropylbutamide, menthol, or menthone.

[0024] Furthermore, the applicant has discovered that the addition of an organic acid to a base liquor containing a cooling agent has favorable effects on regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality.

[0025] The organic acid is 150 ppm to 30,000 ppm.Furthermore, the organic acid is 1,000 ppm to 10,000 ppm.Furthermore, the organic acid is 1,000 ppm to 6,000 ppm.

[0026] The organic acid is selected from at least one of formic acid, acetic acid, propionic acid, lactic acid, butanoic acid, succinic acid, pentanoic acid, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, citric acid, malic acid, tartaric acid, oxalic acid, oleic acid, linoleic acid, palmitic acid, or lauric acid.

[0027] The compositions of the present invention also include water to adjust the alcohol concentration.

[0028] The method for preparing the composition of the present invention comprises the steps of: dissolving a cooling agent in alcohol, then adding water and mixing the mixture thoroughly. Alternatively, it comprises dissolving a cooling agent in alcohol, then adding an organic acid, and finally adding water and mixing the mixture thoroughly.

[0029] The test results show that healthy adults who drink a certain amount of alcohol, cooling agent, or organic acid alone for a short period of 5 to 7 days have no significant effect on regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immune and sleep quality. Drinking a certain amount of a composition of cooling agent and organic acid, or a certain amount of a composition of alcohol and organic acid for a short period of time has no significant effect on regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immune and sleep quality. However, drinking a certain amount of a composition of alcohol and cooling agent for a short period of time has an improving effect on regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immune and sleep quality. Drinking a certain amount of a composition of alcohol, a cooling agent and an organic acid in a short period of time has significant improving effects on regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality.

[0030] The composition of the present application is used for regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality. This is mainly related to the intake of alcohol and cooling agent in the composition by the human body. In terms of the taste adaptation of the human body and the alcohol tolerance of the human body, the intake of the composition may vary depending on the content of alcohol and cooling agent in the composition. When the alcohol and cooling agent in the composition is low, the intake of the composition can be increased to increase the intake of alcohol and cooling agent. This plays a role in regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality. When the alcohol and cooling agent in the composition is high, the composition can be diluted with water to meet the individual requirements of the human body regarding taste adaptation and alcohol tolerance, and obtain the effects of regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality.

[0031] Table A gives recommended intake ranges.

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[0032] Clinical studies have shown that long-term excessive alcohol intake can cause sleep and sexual dysfunction, as well as the development of cardiovascular disease and alcoholic liver damage, and even the development of tumors, which have a major impact on the immune system of the human body. The depressant effect of alcohol on the central nervous system refers to its stimulatory effect, with increasing doses, from the cerebral cortex down to the limbic system, cerebellum, reticular formation, and medulla oblongata. This is due to the fact that alcohol acts on the postsynaptic membrane gamma-aminobutyric acid (GABA) receptors in the brain, thereby suppressing the inhibitory effect of GABA in the brain. As the alcohol concentration increases, alcohol binds to more receptors, thereby causing a wide range of effects. Studies have shown that alcohol reduces immune function, suppresses interferon secretion, and interferes with the responsiveness of the original antibody to newly introduced antigens. Studies have shown that long-term heavy drinking can cause sexual dysfunction in rats. This is due to the fact that alcohol suppresses the hypothalamic-pituitary-gonadal axis by lowering serum levels, thus directly suppressing sexual dysfunction in rats. In addition, alcohol suppression may further cause penile smooth muscle atrophy by suppressing choline acetyltransferase activity in penile tissue, thus indirectly suppressing sexual function in rats.

[0033] Research shows that menthol derivatives can shorten sleep latency and antagonize the central stimulant effect of thiosemicarbazide to improve sleep quality, and at the same time, L-menthol and its derivatives can significantly suppress the expression levels of glutamate receptors and acetylcholinesterase in the hippocampus region of rats, thereby improving their cognitive ability and memory. In addition, research also shows that L-menthol and its derivatives have antidepressant, anticonvulsant and hypnotic effects on organisms. Researchers have found that some menthol derivatives can activate TRPM8, and they have higher efficacy and specificity than menthol, while having high development potential. Research shows that TRPM8 activation can improve vascular pressure and prevent hypertension. TRPM8 is widely distributed. In addition to TRPM8 expressed in prostate cancer, TRPM8 is also expressed in some non-prostate primary tumors and is associated with tumor cell survival and proliferation. It is likely to be a new target for antitumor therapy.

[0034] Organic acids can regulate the cellular immune system and induce host cells to produce antimicrobial peptides to achieve indirect bacterial inhibition, thereby providing immunity to the body. Meanwhile, studies have reported that succinic acid has a regulatory effect on both the GABA and Glu neurotransmitter systems in the CAI region of the rat hippocampus, and can act on the GABA system through a calcium ion-centered regulatory mechanism.

[0035] The present invention first investigated the useful effects of a composition of alcohol and a cooling agent in regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality, particularly the useful effects of a composition of alcohol, a cooling agent, and an organic acid in regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality. According to the present invention, three types of immunoglobulins (IgA, IgG, IgM), serotonin (5-HT), estradiol (E2), testosterone (T), and nitric oxide (NO) in blood are measured to evaluate the function of the composition in regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality. However, in men, interferon-gamma (IFN-γ) should be measured, and in women, progesterone (P), dopamine (DA), and endorphins (β-EP) should be measured. Also, the rate of deep sleep in humans should be measured.

[0036] Immunoglobulins are important substances for anti-infection in the body. It is important to maintain a good level of immunoglobulins against viral and bacterial infections. Clinically, immunoglobulins IgA, IgG and IgM are often tested. The concentrations of IgA, IgG and IgM represent the level of humoral immune function, with high concentrations indicating strong humoral immune function.

[0037] Interferon-γ (IFN-γ) is a representative cytokine secreted by T lymphocytes, which has antiviral and immunomodulatory effects on immune and inflammatory responses, tumor prevention and cell division inhibition. IFN-γ has become a widely relevant antitumor drug due to its antitumor cell proliferation, tumor angiogenesis inhibition and immunomodulatory effects. IFN-γ can significantly inhibit the growth of various tumors and has been applied in clinical trials. IFN-γ can assist the body's immune system in recognizing and killing tumors by increasing the expression levels of adhesion molecules and MHC molecules on the surface of tumor cells and antigen-presenting cells, and promote the recruitment of more immune cells, thereby exerting good antitumor effects. Research has shown that in the tumor microenvironment, IFN-γ not only activates CD4+Th1 cells to exert its immune function, but also inhibits the proliferation of cancer cells, promotes apoptosis of cancerous cells, directly kills cancer cells, and promotes high expression levels of major histocompatibility complex class I molecules in cancer cells, resulting in the recognition of cancer cells by specific T cells.

[0038] Serotonin (5-HT) is an important immune regulator. It can promote killing by natural killer (NK) cells related to anti-tumor, anti-virus, etc., enhance the phagocytosis of macrophages, and promote the proliferation and activation of T cells. As an amine neurotransmitter, serotonin is a natural mood stabilizer, which is important for us to obtain a sense of happiness, emotional calm, and focused attention. At the same time, recent studies have found that 5-HT also promotes cell mitosis and is involved in tissue repair. In recent years, many studies have found that 5-HT plays an important role in regulating the proliferation of hepatocytes during liver regeneration. Platelets are the main carriers of 5-HT in the blood and can transport 5-HT to various organs.

[0039] Estradiol is an estrogen, and high estradiol can suppress the synthesis and secretion of androgens by the testes, which causes sexual dysfunction due to insufficient androgen secretion. Estrogen maintains the thickness and elasticity of the vaginal mucosa and vaginal lubrication during sexual arousal.

[0040] Androgens and estrogens are important for the development, function, metabolism and aging of male and female reproductive organs. Maintaining a high testosterone / estradiol ratio (T / E2) in the male body is crucial for erectile function, spermatogenesis and fertility. A woman's sexual desire is determined primarily by testosterone (T). Testosterone supplements can increase a woman's sexual desire and good mood. When the testosterone / estradiol (T / E2) ratio is increased, testosterone is a very effective aphrodisiac for women.

[0041] Nitric oxide (NO) is a serum indicator related to erectile function, which can relax cavernous vascular smooth muscle and cause erection. It is an important substance to ensure male erectile function. Nitric oxide can increase genital blood flow and play an important role in maintaining normal sexual function. It can cause clitoral and vaginal engorgement, increasing clitoral sensitivity and vaginal lubrication. Meanwhile, it is also closely related to the cardiovascular and cerebrovascular systems. Endothelium-derived relaxing factor (EDRF), first discovered in 1980, is now confirmed to be NO. It has vasodilatory properties and acts as a messenger for intracellular and intercellular transmission of biological information in physiological conditions such as ischemia. Research has shown that nitric oxide contacts and relaxes and dilates arterial smooth muscle cells, resulting in a reduction in hypertension and improving blood flow. In addition, nitric oxide prevents blood clotting in several dangerous areas. If blood clots in the heart or brain, the patient will suffer from heart disease or stroke. Assuming the body produces sufficient amounts of nitric oxide, the risk of heart disease is significantly reduced. Therefore, nitric oxide can effectively lower blood pressure and prevent stroke and heart disease.

[0042] Progesterone increases sexual desire in women, and dopamine increases sexual desire and arousal.

[0043] Serum endorphins (β-EP) are very important neuromodulators in the body, known as the body's natural opium and "pleasure hormone". It helps people stay youthful and happy. At the same time, it can regulate the endocrine system in women, reduce depression and increase pleasure.

[0044] Sleep is extremely important for protecting human health and maintaining normal physiological and psychological activity. Sleep quality also plays a major role in protecting the body's immune function.

[0045] Many studies have shown that the parasympathetic nervous system plays an important regulatory role in the nervous system, endocrine system and immune system, and its basic functions include increasing the secretion of salivary glands, gastrointestinal tract, liver, bile and pancreas, promoting the secretion of digestive glands to increase intestinal peristalsis, promoting nutrient and energy absorption, promoting insulin secretion by pancreatic Langerhans islet cells, promoting the production of liver glycogen to increase energy storage, narrowing the pupils to reduce irritation, lowering heart rate, lowering blood pressure, etc. to save unnecessary consumption, participating in endocrine regulation, supporting reproductive activity to cause genital vascular dilation and erection, and increased genital secretion. Research has also shown that the action potential of the parasympathetic nerve can directly cause male erection. In summary, the parasympathetic nerve helps to relax our body, which allows us to perceive a state of calm, serenity, joy and relaxation, thereby increasing energy storage and fatigue recovery to achieve body maintenance, recovery and protection. In addition, this helps restore the constantly over-excited sympathetic nervous system to a normal state, improving the body's chronic state of stress.

[0046] When the parasympathetic nerve is stimulated, it releases a large amount of acetylcholine (Ach) as a transmitter of nerve signals. Acetylcholine is transmitted to target organs through the parasympathetic nerve. M receptors are short for muscarinic receptors and are widely found on effector cells innervated by postganglionic fibers of the parasympathetic nerve. M receptors receive signals (acetylcholine) from the parasympathetic nerve, generate a series of biochemical reactions inside the cell, and present an excitatory effect at the sympathetic nerve ending. In contrast, M receptors achieve the excitatory effect of the parasympathetic nerve through the PLC / PIP2 / IP3 signaling pathway.

[0047] Intracellular M receptors are coupled to G proteins, and the Gαq protein-coupled receptor-mediated phospholipase C (PLC) signaling system is a widespread signaling mechanism. After activation of Gαq protein, PLCβ is activated, and then phosphatidylinositol-4,5-bisphosphate (PIP2) is hydrolyzed on the cell membrane into two secondary messengers, namely inositol triphosphate (IP3) and diacylglycerol pyrophosphate (DAG). M3 receptors are widely found in the endoplasmic reticulum membrane and function as channel proteins that can induce intracellular calcium mobilization. It can bind to IP3 and induce intracellular calcium mobilization, causing an increase in intracellular calcium levels. This initiates the intracellular calcium signaling system. In addition, a series of physiological processes, such as ion channel function, cell cycle, glandular secretion, smooth muscle contraction, vasodilation, and genital erection, are regulated through changes in enzyme activity, such as calmodulin of calcium ions. Thus, M3 receptors achieve parasympathetic excitatory effects via the PLC / PIP2 / IP3 signaling pathway.

[0048] Through experiment, it is found that the composition of the present invention can increase the expression level of muscarinic acetylcholine receptor (M3 receptor) and the content of PLC and IP3 in PLC / PIP2 / IP3 signaling pathway.This shows that the function of the composition that regulates sexual function, protects cardiovascular system and cerebrovascular system, promotes liver cell regeneration, prevents tumor, and improves immunity and sleep quality is regulated through parasympathetic nerve signaling pathway.

[0049] The following further describes the embodiments of the present invention through specific embodiments, but this does not indicate that the protection scope of the present invention is limited to the scope described in the embodiments. EXAMPLES

[0050] Example 1 Preparation of Composition 1 of the Present Invention Composition 1 includes ingredients such as a cooling agent prepared from menthyl acetate, L-menthyl lactate, and monomethyl succinate in a weight ratio of 4:5:1, organic acids prepared from acetic acid, lactic acid, propionic acid, butanoic acid, and hexanoic acid in a weight ratio of 14:15:1.5:1:1, and 4.5% by volume of beer as a base liquor.

[0051] Composition 1, which contains 1% by volume of alcohol, 0.05 ppm of cooling agent, and 150 ppm of organic acid, was prepared from a base liquor blended with the cooling agent, organic acid, and purified water.

[0052] Example 2 Preparation of Composition 2 of the Present Invention Composition 2 includes ingredients such as a cooling agent prepared from monomethyl succinate, L-monomethyl glutarate, and N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide in a weight ratio of 2:5:3, organic acids prepared from acetic acid, lactic acid, propionic acid, succinic acid, pentanoic acid, and heptanoic acid in a weight ratio of 7:8:1:2:1:1, and 40% by volume of whiskey as a base liquor.

[0053] Composition 2, which contains 30% by volume of alcohol, 10 ppm of cooling agent, and 1000 ppm of organic acid, was prepared from a base liquor blended with the cooling agent, organic acid, and purified water.

[0054] Example 3 Preparation of Composition 3 of the Invention Composition 3 includes ingredients such as a cooling agent prepared by N,2,3-trimethyl-2-isopropylbutamide, L-menthyl lactate, monomethyl succinate and L-menthol ethylene glycol carbonate in a weight ratio of 2:3:2:3, organic acids prepared by formic acid, acetic acid, lactic acid, propionic acid, octanoic acid, nonanoic acid and decanoic acid in a weight ratio of 1:8:5:2:1:1:1:, and 60% by volume of Chinese baijiu as a base liquor.

[0055] Composition 3, with 60% by volume alcohol, 100 ppm cooling agent, and 6000 ppm organic acid, was prepared with the cooling agent, the organic acid blended with the base liquor.

[0056] Example 4 Preparation of Composition 4 of the Present Invention Composition 4 includes ingredients such as a cooling agent prepared by monomethyl succinate, L-monomethyl glutarate and N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide in a weight ratio of 3:4:3, organic acids prepared by acetic acid, lactic acid, propionic acid, citric acid, malic acid and tartaric acid in a weight ratio of 5:5:1:2:1:1, and 12% by volume of Chinese baijiu as a base liquor.

[0057] Composition 4, which contains 80% by volume of alcohol, 200 ppm of cooling agent, and 10,000 ppm of organic acid, was prepared from a base alcohol blended with the cooling agent, organic acid, and edible alcohol.

[0058] Example 5 Preparation of Composition 5 of the Present Invention Composition 5 includes ingredients such as a cooling agent prepared by N,2,3-trimethyl-2-isopropylbutamide, L-menthyl lactate, 3-L-menthoxypropane-1,2-diol, L-menthol 1-(or 2-)-propylene glycol carbonate, and L-monomethyl glutarate in a weight ratio of 4:1:2:3:1, an organic acid prepared by lactic acid, propionic acid, hexanoic acid, citric acid, and malic acid in a weight ratio of 5:1:1:2:2, and 99% by volume of dehydrated edible alcohol as a base alcoholic beverage.

[0059] Composition 5, with 99% by volume alcohol, 500 ppm cooling agent, and 30,000 ppm organic acid, was prepared with the cooling agent, the organic acid blended with the base liquor.

[0060] Example 6 Preparation of Composition 6 of the Present Invention Composition 6 includes ingredients such as a cooling agent prepared by menthyl acetate, L-menthyl lactate, and N,2,3-trimethyl-2-isopropylbutamide in a weight ratio of 2:3:5, organic acids prepared by acetic acid, succinic acid, hexanoic acid, citric acid, and malic acid in a weight ratio of 3:1:1:4:2, and 50% by volume of Chinese baijiu as a base liquor.

[0061] Composition 6, having 50% by volume alcohol, 50 ppm cooling agent, and 3000 ppm organic acid, was prepared with the cooling agent, organic acid blended with the base liquor.

[0062] Example 7 Preparation of Composition 7 of the Invention Composition 7 includes ingredients such as 150 ppm cooling agent prepared by L-menthyl lactate and monomethyl succinate in a weight ratio of 4:6, organic acids prepared by acetic acid, lactic acid, succinic acid, hexanoic acid and tartaric acid in a weight ratio of 3:3:1:1:2, and 70% by volume of edible alcohol as the base alcohol.

[0063] Composition 7, having 70% by volume alcohol, 150 ppm cooling agent, and 8000 ppm organic acid, was prepared with the cooling agent, organic acid blended with the base liquor.

[0064] Comparative Example 1 Preparation of low-alcohol composition 8 Composition 8 includes ingredients such as a cooling agent prepared from menthyl acetate, L-menthyl lactate, and monomethyl succinate in a weight ratio of 4:5:1, organic acids prepared from acetic acid, lactic acid, propionic acid, butanoic acid, and hexanoic acid in a weight ratio of 14:15:1.5:1:1, and 15% by volume of sake as a base sake.

[0065] Composition 8, which had 0.5% by volume of alcohol, 5 ppm of cooling agent, and 500 ppm of organic acid, was prepared from a base liquor blended with the cooling agent, organic acid, and purified water.

[0066] Comparative Example 2 Preparation of low cooling sensation agent composition 9 Composition 9 contains ingredients such as a cooling agent prepared from monomethyl succinate, L-monomethyl glutarate and N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide in a weight ratio of 2:5:3, organic acids prepared from acetic acid, lactic acid, propionic acid, succinic acid, pentanoic acid and heptanoic acid in a weight ratio of 7:8:1:2:1:1, and 53 volume% of Jiang-flavored white spirit as the base liquor.

[0067] Composition 9, having 53% by volume alcohol, 0.04 ppm cooling agent, and 2000 ppm organic acid, was prepared with the cooling agent, organic acid blended with the base liquor.

[0068] Example 8 Preparation of low acid composition 10 Composition 10 includes ingredients such as a cooling agent prepared by N,2,3-trimethyl-2-isopropylbutamide, L-menthyl lactate, monomethyl succinate, and L-menthol ethylene glycol carbonate in a weight ratio of 2:3:2:3, organic acids prepared by formic acid, acetic acid, lactic acid, propionic acid, octanoic acid, nonanoic acid, and decanoic acid in a weight ratio of 1:8:5:2:1:1:1:, and 50% by volume of edible alcohol as a base liquor.

[0069] A composition 10 having 50% by volume alcohol, 50 ppm cooling agent, and 100 ppm organic acid was prepared with the cooling agent, organic acid blended with the base liquor.

[0070] Comparative Example 3 Preparation of Alcohol-Free Composition 11 Composition 11 includes ingredients such as a cooling agent prepared by N,2,3-trimethyl-2-isopropylbutamide, L-monomethyl glutarate, monomethyl succinate, and L-menthol ethylene glycol carbonate in a weight ratio of 2:3:3:1, and an organic acid prepared by acetic acid, lactic acid, propionic acid, citric acid, malic acid, and tartaric acid in a weight ratio of 5:5:1:2:1:1.

[0071] Composition 11, with 5 ppm cooling agent and 1000 ppm organic acid, was prepared with the cooling agent, organic acid blended with purified water.

[0072] Comparative Example 4 Preparation of Composition 12 without Cooling Agent Composition 12 includes ingredients such as organic acids prepared by acetic acid, succinic acid, hexanoic acid, citric acid and malic acid in a weight ratio of 3:1:1:4:2, and 53 volume% of Jiang-type white spirit as the base liquor.

[0073] Composition 12, 53% by volume alcohol and 2000 pm organic acid, was prepared with the above organic acid blended with the base liquor.

[0074] Example 9 Preparation of Composition 13 without Organic Acid Composition 13 contains ingredients such as a cooling agent prepared from N,2,3-trimethyl-2-isopropylbutamide, 3-L-menthoxypropane-1,2-diol, L-menthol 1-(or 2-)-propylene glycol carbonate and L-monomethyl glutarate in a weight ratio of 4:1:2:3, and 60% by volume of edible alcohol as a base liquor.

[0075] Composition 13, 60% by volume alcohol and 100 ppm cooling agent, was prepared with the cooling agent blended with the base liquor.

[0076] Example 10 Preparation of Composition 14 without Organic Acid Composition 14 includes ingredients such as a cooling agent prepared from menthyl acetate, monomethyl succinate, menthol and menthone in a weight ratio of 10:8:2:1, and 1% by volume of edible alcohol as a base liquor.

[0077] Composition 14, having 1% alcohol by volume and 0.05 ppm cooling agent, was prepared with the cooling agent blended with the base liquor.

[0078] Example 11 Preparation of Composition 15 without Organic Acid Composition 15 includes ingredients such as a cooling agent prepared from menthyl acetate, L-menthyl lactate, monomethyl succinate, L-monomethyl glutarate, and L-menthol ethylene glycol carbonate in a weight ratio of 3:2:1:2:2, and 30% by volume of edible alcohol as a base liquor.

[0079] Composition 15, 30% by volume alcohol and 10 ppm cooling agent, was prepared with the cooling agent blended with the base liquor.

[0080] Example 12 Preparation of Composition 16 without Organic Acid Composition 16 includes ingredients such as monomethyl succinate, 3-L-menthoxy-2-methylpropane-1,2-diol, a cooling agent prepared from N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide and 2-isopropyl-N-2,3-trimethylbutyramide in a weight ratio of 2:1:3:1, and 99% by volume edible alcohol as the base liquor.

[0081] Composition 16, 99% by volume alcohol and 500 ppm cooling agent, was prepared with the cooling agent blended with the base liquor.

[0082] The amounts of alcohol, cooling agent and organic acid in the above composition are shown in Table B.

number

[0083] Verification of effectiveness The improvement of sexual function, cardiovascular and cerebrovascular systems, liver cell regeneration, tumor prevention, immunity and sleep quality are evaluated for the indicators of the compositions prepared in the examples and comparative examples. The specific methods are as follows:

[0084] Subjects: 31 healthy men (aged 19-56 years) and 36 healthy women (aged 25-50 years). The participants were uniformly enrolled in the study after fully understanding the test alcohol samples and the test methods and requirements, and signing an informed consent form.

[0085] Drinking amount and time: 50mL / person / day, consumed at dinner for 7 consecutive days. From the first 7 days before drinking, other alcoholic beverages, functional beverages, and dietary supplements were discontinued until various indicators were tested at the end of drinking. A before-and-after comparative method was used.

[0086] Test Index Serological indices: Blood samples were taken before and 7 days after the ingestion of the tea, and the following were tested for men: three types of immunoglobulins (IgA, IgG, IgM), interferon-gamma (IFN-gamma), serotonin (5-HT), estradiol (E2), testosterone (T), and nitric oxide (NO). For women, the following were tested: three types of immunoglobulins (IgA, IgG, IgM), serotonin (5-HT), estradiol (E2), testosterone (T), progesterone (P), nitric oxide (NO), dopamine (DA), and endorphins (β-EP).

[0087] Blood sample testing institution: Chengdu Lilai Biotechnology Co., Ltd.

[0088] Evaluation of the sleep bracelet and scale: The sleep monitoring bracelet (Huawei Bracelet 4) monitored daily sleep data from 2 days before drinking to 2 days after drinking. The purpose of monitoring for 2 days before drinking was to obtain the average data for the 2 days and to avoid abnormal data or incomplete recording of the bracelet.

[0089] Statistical method: Software SPSS 22.0, paired T-test

[0090] According to the above test information, the serum immunoglobulin index of men using the compositions prepared in the examples and comparative examples was tested, and the results shown in Table 1 were obtained. [Table 1] TIFF2024523005000004.tif134145

[0091] According to the above test results, it was found that the immunoglobulin levels of the test groups drinking the compositions in the examples increased to various degrees, and the immunoglobulin levels of the low-acid composition 10 and the organic acid-free compositions 13-16 also increased slightly, but their increase rates were inferior to those of the compositions 1-7. However, no significant difference was observed in the immunoglobulin levels before and after drinking the alcohol-free composition, low-alcohol composition, cooling agent-free composition, and low cooling agent composition of the comparative examples. It has been demonstrated that the composition of the present invention increases the immunoglobulin levels of men, thereby indicating that the composition can enhance immunity by increasing the immunoglobulin levels of men.

[0092] According to the above test information, the serum immunoglobulin index of women who had been administered the compositions prepared in the examples and comparative examples was tested, and the results shown in Table 2 were obtained. [Table 2] TIFF2024523005000006.tif240147

[0093] According to the above test results, it was found that the immunoglobulin levels of the test groups drinking the compositions in the examples increased to various degrees, and the immunoglobulin levels of the low-acid composition 10 and the organic acid-free compositions 13-16 also increased slightly, but their increase rates were inferior to those of the compositions 1-7. However, no significant difference was observed in the immunoglobulin levels before and after drinking the alcohol-free composition, low-alcohol composition, cooling agent-free composition, and low cooling agent composition of the comparative examples. It has been demonstrated that the composition of the present invention increases the immunoglobulin levels of women, thereby indicating that the composition can enhance immunity by increasing the immunoglobulin levels of women.

[0094] According to the above test information, the interferon-γ (IFN-γ) index of men was tested using the compositions prepared in the examples and comparative examples, and the results shown in Table 3 were obtained. [Table 3]

[0095] According to the above test results, it was found that the IFN-γ levels of the test groups drinking the compositions in the examples increased, and the low acidity composition 10 and the organic acid-free compositions 13-16 also increased slightly, but the increase rates were inferior to those of compositions 1-7. However, no significant difference was observed in the IFN-γ levels before and after drinking the alcohol-free composition, low alcohol composition, cooling agent-free composition, and low cooling agent composition of the comparative examples. It has been demonstrated that the composition of the present invention increases IFN-γ in men, thereby indicating that the composition can prevent tumors and strengthen immunity by increasing the IFN-γ levels in men.

[0096] According to the above test information, the serotonin (5-HT) index of males was tested using the compositions prepared in the examples and comparative examples, and the results shown in Table 4 were obtained. [Table 4]

[0097] According to the above test results, it was found that the serotonin (5-HT) levels of the test group drinking the composition in the embodiment were significantly increased, and the low acid composition 10 and the compositions 13-16 without organic acid also increased slightly, but their increase rates were inferior to those of the compositions 1-7. However, there was no significant difference in the serotonin (5-HT) levels before and after drinking the alcohol-free composition, the low alcohol composition, the composition without cooling agent, and the low cooling agent composition of the comparative example. It is proved that the composition of the present invention increases the serotonin (5-HT) levels of men, thereby indicating that the composition can promote liver cell regeneration and strengthen immunity by increasing the 5-HT levels of men.

[0098] According to the above test information, the serotonin (5-HT) index of women was tested using the compositions prepared in the Examples and Comparative Examples, and the results shown in Table 5 were obtained. [Table 5]

[0099] According to the above test results, it was found that the serotonin (5-HT) levels of the test group drinking the composition in the embodiment were significantly increased, and the low acidity composition 10 and the organic acid-free compositions 13-16 also showed a slight increase, but their increase rates were inferior to those of compositions 1-7. However, no significant difference was observed in the serotonin (5-HT) levels before and after drinking the alcohol-free composition, low alcohol composition, cooling agent-free composition, and low cooling agent composition of the comparative examples. It has been demonstrated that the composition of the present invention increases the serotonin (5-HT) levels of women, thereby indicating that the composition can induce liver cell regeneration and strengthen immunity by increasing the 5-HT levels of women.

[0100] According to the above test information, the estradiol (E2) index of males was tested using the compositions prepared in the examples and comparative examples, and the results shown in Table 6 were obtained. [Table 6]

[0101] According to the above test results, it was found that the estradiol (E2) levels of the test group drinking the compositions in the examples were reduced, and the low acidity composition 10 and the organic acid-free compositions 13-16 also slightly decreased, but the reduction rates were inferior to those of compositions 1-7. However, no significant difference was observed in the estradiol (E2) levels before and after drinking the alcohol-free composition, low-alcohol composition, the cooling agent-free composition, and the low cooling agent composition of the comparative examples. It is demonstrated that the composition of the present invention reduces the estradiol (E2) levels in men, thereby indicating that the composition can regulate sexual function by reducing the E2 levels in men.

[0102] According to the above test information, the estradiol (E2) index of women was tested using the compositions prepared in the examples and comparative examples, and the results shown in Table 7 were obtained. [Table 7]

[0103] According to the above test results, it was found that the estradiol (E2) levels of the test group drinking the compositions in the examples increased, and the low acidity composition 10 and the organic acid-free compositions 13-16 also increased slightly, but their reduction rates were inferior to those of compositions 1-7. However, no significant difference was observed in the estradiol (E2) levels before and after drinking the alcohol-free composition, low-alcohol composition, the cooling agent-free composition, and the low cooling agent composition of the comparative examples. It has been demonstrated that the composition of the present invention increases the estradiol (E2) levels of women, thereby indicating that the composition can regulate sexual function by increasing the E2 levels of women.

[0104] According to the above test information, the testosterone / estradiol (T / E2) index of men was tested using the compositions prepared in the examples and comparative examples, and the results shown in Table 8 were obtained. [Table 8]

[0105] According to the above test results, it was found that the test group drinking the composition in the embodiment had an increased testosterone / estradiol (T / E2) ratio, and the low acid composition 10 and the organic acid-free compositions 13-16 also had a slight increase, but their increase rates were inferior to those of compositions 1-7. However, there was almost no increase in the testosterone / estradiol (T / E2) ratio before and after drinking the alcohol-free composition, low alcohol composition, the cooling agent-free composition, and the low cooling agent composition of the comparative example. It is demonstrated that the composition of the present invention increases the testosterone / estradiol (T / E2) ratio in men to some extent, thereby indicating that the composition can regulate sexual function by increasing the testosterone / estradiol (T / E2) ratio in men.

[0106] According to the above test information, the testosterone / estradiol (T / E2) index of women was tested using the compositions prepared in the examples and comparative examples, and the results shown in Table 9 were obtained. [Table 9]

[0107] According to the above test results, it was found that the test group drinking the composition in the embodiment had an increased testosterone / estradiol (T / E2) ratio, and the low acidity composition 10 and the organic acid-free compositions 13-16 also had a slight increase. However, there was almost no increase in the testosterone / estradiol (T / E2) ratio before and after drinking the alcohol-free composition, low-alcohol composition, cooling agent-free composition, and low cooling agent composition of the comparative example. It is demonstrated that the composition of the present invention increases the testosterone / estradiol (T / E2) ratio in women to some extent, thereby indicating that the composition can regulate sexual function by increasing the testosterone / estradiol (T / E2) ratio in women.

[0108] According to the above test information, the nitric oxide (NO) index of men was tested using the compositions prepared in the Examples and Comparative Examples, and the results shown in Table 10 were obtained. [Table 10]

[0109] According to the above test results, it was found that the nitric oxide level of the test group drinking the composition in the embodiment significantly increased, and the low acid composition 10 and the compositions 13-16 without organic acid also increased slightly, but the increase rate was inferior to that of the compositions 1-7. However, there was almost no increase in the nitric oxide level before and after drinking the alcohol-free composition, the low alcohol composition, the composition without cooling agent, and the low cooling agent composition of the comparative example. It has been demonstrated that the composition of the present invention can effectively increase the nitric oxide level in men, thereby indicating that the composition can regulate sexual function and protect the cardiovascular system and cerebrovascular system by increasing the nitric oxide level in men.

[0110] According to the above test information, the nitric oxide (NO) index of women was tested using the compositions prepared in the Examples and Comparative Examples, and the results shown in Table 11 were obtained. [Table 11]

[0111] According to the above test results, it was found that the nitric oxide level of the test group drinking the composition in the embodiment was significantly increased, while the low acid composition 10 and the compositions 13 to 16 without organic acid showed no significant increase. However, there was almost no increase in the nitric oxide level before and after drinking the alcohol-free composition, the low alcohol composition, the composition without cooling agent, and the low cooling agent composition of the comparative example. It has been demonstrated that the composition of the present invention can effectively increase the nitric oxide level in women, thereby indicating that the composition can regulate sexual function and protect the cardiovascular and cerebrovascular systems by increasing the nitric oxide level in women.

[0112] According to the above test information, the progesterone (P) index of women who used the compositions prepared in the examples and comparative examples was tested, and the results shown in Table 12 were obtained. [Table 12]

[0113] According to the above test results, it was found that the progesterone (P) level of the test group drinking the composition in the embodiment was significantly increased, and the low acid composition 10 and the compositions 13-16 without organic acid also increased slightly, but the increase rate was inferior to that of the compositions 1-7. However, there was almost no increase in the progesterone (P) level before and after drinking the alcohol-free composition, the low alcohol composition, the composition without cooling agent, and the low cooling agent composition of the comparative example. It is proved that the composition of the present invention can effectively increase the progesterone (P) level of a woman, thereby indicating that the composition can regulate sexual function by increasing the progesterone (P) level of a woman.

[0114] According to the above test information, the dopamine (DA) index of females was tested using the compositions prepared in the Examples and Comparative Examples, and the results shown in Table 13 were obtained. [Table 13]

[0115] According to the above test results, it was found that the dopamine (DA) levels of the test group drinking the composition in the embodiment were significantly increased, and the low acid composition 10 and the compositions 13-16 without organic acid also increased, but the increase rates were inferior to those of the compositions 1-7. However, there was almost no increase in the dopamine (DA) levels before and after drinking the alcohol-free composition, the low alcohol composition, the composition without cooling agent, and the low cooling agent composition of the comparative example. It is proved that the composition of the present invention can effectively increase the dopamine (DA) level in women, thereby indicating that the composition can regulate sexual function by increasing the dopamine (DA) level in women.

[0116] According to the above test information, the endorphin (β-EP) index of women was tested using the compositions prepared in the examples and comparative examples, and the results shown in Table 14 were obtained. [Table 14]

[0117] According to the above test results, it was found that the endorphin (β-EP) levels of the test group drinking the composition in the embodiment were significantly increased, and the low acid composition 10 and the compositions 13 to 16 without organic acid also increased, but the increase rates were inferior to those of the compositions 1 to 7. However, there was almost no increase in the endorphin (β-EP) levels before and after drinking the alcohol-free composition, the low-alcohol composition, the composition without cooling agent, and the low cooling agent composition of the comparative examples. It has been demonstrated that the composition of the present invention can effectively increase the endorphin (β-EP) levels of women, thereby indicating that the composition can regulate sexual function by increasing the β-EP levels of women.

[0118] According to the above test information, the deep sleep rate of humans was tested using the compositions prepared in the Examples and Comparative Examples, and the results shown in Table 15 were obtained. [Table 15]

[0119] According to the above test results, it was found that the test groups drinking the compositions in the examples had a deep sleep ratio that increased to various extents, and the low acid composition 10 and the organic acid-free compositions 13-16 also increased, but the increase rates were inferior to those of the compositions 1-7. However, no significant difference was observed in the deep sleep ratio before and after drinking the alcohol-free composition, low alcohol composition, cooling agent-free composition, and low cooling agent composition of the comparative examples. It has been demonstrated that the composition of the present invention can increase the deep sleep ratio of humans, thereby indicating that the composition can improve the quality of sleep by increasing the deep sleep ratio of humans.

[0120] From the above data, it can be seen that the composition of the present invention has the functions of regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality, while increasing and sustaining saliva secretion.Analysis of this phenomenon shows that the functions of the composition regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing tumors, and improving immunity and sleep quality are regulated by activating the parasympathetic signaling pathway.Therefore, the expression level of M3 receptor and the content of PLC and IP3 in the signaling pathway being tested can involve the activation of the parasympathetic signaling pathway.The test method is as follows:

[0121] After 7 days of acclimation feeding, SPF male mice were randomized into groups of 10 mice each according to a randomization formula: blank control group: saline group, test group 1: composition 6, test group 2: 50% by volume of pure edible alcohol, test group 3: aqueous solution containing 50 ppm of cooling agent, test group 4: aqueous solution containing 3000 ppm of organic acid.

[0122] Administration method: 15mL / kg of saline and each test sample were drawn into a syringe and slowly dripped into the oral cavity of the mouse in small amounts. The mice were administered once a day for three consecutive days.

[0123] On the third day, 30 minutes after drug administration, the mice were sacrificed by spinal dislocation, and the submandibular gland tissues of the mice were homogenized with a homogenizer and centrifuged at 7000r / min for 5 minutes, and the supernatant was collected. Enzyme-linked immunosorbent (ELISA) assay was used to determine M3, PLC and IP3. The results shown in Table 16 were obtained.

[0124] [Table 16]

[0125] According to the above test results, the test group 1, which drank the composition 6, had higher expression levels of M3, PLC and IP3 than the control group (p<0.05), and no significant difference was observed between the test groups 2, 3, 4, which contained only alcohol, cooling agent or organic acid, and the control group. This indicates that the composition of the present invention can increase the expression level of M3 receptor and activate the PLC / IP3 pathway of the parasympathetic nervous system.

[0126] Through the above test results, it can be verified that the composition of the present invention can play roles such as regulating sexual function, protecting the cardiovascular system, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality by activating the parasympathetic nervous signaling pathway, and can be widely used in the fields of medicine, food and health care products.

Claims

1. 1. Use of a composition of alcohol, a cooling agent, and an organic acid in the preparation of a composition for regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality, comprising: The alcohol is selected from distilled spirits or edible alcohol; the cooling agent comprises at least one of menthyl acetate, L-menthyl lactate, monomenthyl succinate, L-monomenthyl glutarate, L-menthol ethylene glycol carbonate, L-menthol 1-(or 2-)-propylene glycol carbonate, 3-L-menthoxypropane-1,2-diol, 3-L-menthoxy-2-methylpropane-1,2-diol, N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide, N,2,3-trimethyl-2-isopropylbutamide, menthol, or menthone; the organic acid is selected from at least one of formic acid, acetic acid, propionic acid, lactic acid, butanoic acid, succinic acid, pentanoic acid, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, citric acid, malic acid, tartaric acid, oxalic acid, oleic acid, linoleic acid, palmitic acid, or lauric acid; The content of the alcohol in the composition is 50% by volume to 99% by volume, the content of the cooling agent is 0.05 ppm to 500 ppm, and the content of the organic acid is 150 ppm to 30,000 ppm.

2. 2. Use of a composition of alcohol, a cooling agent, and an organic acid in the preparation of a composition for regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality according to claim 1, wherein the content of the alcohol in the composition is 50% by volume to 80% by volume, the content of the cooling agent is 10 ppm to 200 ppm, and the content of the organic acid is 1,000 ppm to 10,000 ppm.

3. 3. Use of a composition of alcohol, a cooling agent, and an organic acid in the preparation of a composition for regulating sexual function, protecting the cardiovascular and cerebrovascular systems, promoting liver cell regeneration, preventing and / or treating tumors, and improving immunity and sleep quality according to claim 2, wherein the content of the alcohol in the composition is 50% by volume to 60% by volume, the content of the cooling agent is 10 ppm to 100 ppm, and the content of the organic acid is 1,000 ppm to 6,000 ppm.