Skin beauty method

Abstract

To give sufficient youthful appearance and improve customer satisfaction.SOLUTION: A multiple-component skin penetration system including a liquid storage section for storing a liquid to be sprayed containing an active ingredient, a spray nozzle for spraying the liquid to be sprayed stored in the liquid storage section, a gas supply section for supplying gas, and a spray control section for instantaneously supplying gas supplied from the gas supply section to the spray nozzle to spray the liquid to be sprayed together with the gas from the spray nozzle, is used to perform spraying of a first liquid to be sprayed containing NMN and spraying of a second liquid to be sprayed containing human stem cell culture medium.SELECTED DRAWING: Figure 3

Description

[Technical field]

[0001] This invention relates to a skin beauty method for performing skin beauty treatment. law Regarding. [Background technology]

[0002] A skin beauty composition containing an acetic acid extract of onion bulbs and baking soda has been proposed as a skin beauty composition that improves wrinkles and has an excellent feel when used, and a skin beauty method (excluding medical procedures) of applying the composition has been proposed (see Patent Document 1). In this skin beauty method, the onion bulb acetic acid extract alone is effective in improving wrinkles, moisturizing, and beautifying the skin (improving skin texture), but it has been found that by adding baking soda, it is possible to achieve even better skin beauty effects (especially improving wrinkles) in a shorter period of time. In the beauty industry, there is a demand for further effects, such as a youthful appearance effect, i.e., a lifting effect that improves facial sagging and wrinkles, in addition to wrinkle improvement, moisturizing effect, and skin beautifying effect. However, the above-mentioned cosmetic methods do not provide a lifting effect. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Patent No. 6973740 Summary of the Invention [Problem to be solved by the invention]

[0004] In view of the above-mentioned problems, an object of the present invention is to provide a sufficient effect of making a person look younger and to improve the satisfaction of the user. [Means for solving the problem]

[0005] This invention relates to a human stem cell culture medium A first active ingredient in liquid or powder form, NMN Either a liquid or powder of the second active ingredient applying a first composition comprising: The other of the first active ingredient and the second active ingredient and applying a second composition containing The first composition does not contain the other active ingredient contained in the second composition, and the second composition does not contain one of the active ingredients contained in the first composition. It is characterized by being a skin beauty method (excluding medical procedures). Effect of the Invention

[0006] This invention provides a skin beauty method that can achieve a sufficient youthful appearance. law can be provided. [Brief description of the drawings]

[0007] [Figure 1] System diagram of a multi-component skin penetration system. [Diagram 2] 1 is a block diagram showing the configuration of a multi-component skin penetration system. [Diagram 3] 1 is a flow chart illustrating the operation of the multiple component skin penetration system. [Figure 4] An illustration of the before and after effects of the multi-ingredient skin penetration system. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0008] In this invention, a first composition containing a first active ingredient and a second composition containing a second active ingredient are applied to the skin separately or in combination. The first active ingredient and the second active ingredient are a human stem cell culture medium, and NMN is the other. As a method of application, it is preferable to adjust the first penetrating agent consisting of the first active ingredient and the second penetrating agent consisting of the second active ingredient, either individually or together, with an adjuster such as purified water to prepare a spray liquid (liquid for application), and spray the liquid onto the skin using a spraying device. The skin referred to here refers to all body skin, including the skin of the face, neck, décolleté, limbs, chest, abdomen, back, pubic area, and head. Hereinafter, an embodiment of the present invention will be described with reference to the drawings.

[0009] FIG. 1 is a diagram showing the system configuration of a multiple component skin penetration system 1. As shown in FIG. The multiple component skin penetration system 1 is comprised of a first penetrating agent 2, a second penetrating agent 3, a conditioning agent 4, and a spray device 5.

[0010] The first penetrating agent 2 is an agent containing a first active ingredient to be penetrated into the skin, and is composed of a liquid or powder. The second penetrating agent 3 is an agent containing a second active ingredient to be penetrated into the skin, and is composed of a liquid or powder.

[0011] In this example, one of the first penetrating agent 2 and the second penetrating agent 3 is NMN powder, and the other is human stem cell culture medium. NMN powder is a powder of nicotinamide mononucleotide (NMN), which is extracted from breast milk, edamame, broccoli, avocado, tomato, and other organisms and powdered using a granulation process such as microgranulation.

[0012] The human stem cell culture liquid is a supernatant obtained when stem cells collected from humans are cultured in a culture liquid. The human stem cell culture supernatant can be a human umbilical cord stem cell culture supernatant obtained from human umbilical cord, a human umbilical cord blood stem cell culture supernatant obtained from human umbilical cord blood, a human adipose stem cell culture supernatant obtained from human fat, a human bone marrow stem cell culture supernatant obtained from human bone marrow fluid, or a supernatant obtained by culturing other human cells.

[0013] The adjusting agent 4 can be purified water or physiological saline. The purified water can be ordinary purified water or sterilized purified water obtained by sterilizing purified water. The physiological saline can be any appropriate one, such as injectable saline obtained by sterilizing purified water through filtration or distillation and removing pyrogens.

[0014] The first penetrant 2 is adjusted with the adjuster 4 and used as the first spray liquid (first application liquid). Similarly, the second penetrant 3 is adjusted with the adjuster 4 and used as the second spray liquid (second application liquid). The first penetrant 2 and the second penetrant 3 can also be mixed and adjusted with the adjuster 4 to be used as a single integrated spray liquid (integrated application liquid).

[0015] The spray liquid (first spray liquid, second spray liquid, or integrated spray liquid) prepared in this manner can have a viscosity of 200 mPa·s or less, preferably 100 mPa·s or less, more preferably 50 mPa·s or less, more preferably 10 mPa·s or less, even more preferably 5 Pa·s or less, more preferably 3 mPa·s or less, even more preferably 2 mPa·s or less, and preferably 1 mPa·s or less. This makes it possible to prevent clogging inside the spray nozzle 12a of the spray device 5 described below when spraying.

[0016] The spray device 5 is a device that sprays pressurized liquid onto a target (human skin in this embodiment) through a nozzle, and will be described in detail below.

[0017] The spray device 5 has a main body 30 for delivering air, a conduit 20 having one end connected to the main body 30, and a handpiece 10 connected to the other end of the conduit 20 and operated by the practitioner.

[0018] The handpiece 10 is a longitudinal unit that can be held by the practitioner, and is provided with a spray cover 11 at the tip and the other end of the conduit 20 connected to the rear end. A liquid input section 15 is provided on the tip side of the upper surface of the handpiece 10 from the center. The liquid input section 15 is provided with a liquid input hole 15b on the cover of the upper surface, and a liquid storage section 15a is provided inside the handpiece 10. The liquid storage section 15a is a sterile room, and the liquid to be sprayed is input into the liquid storage section 15a from the liquid input hole 15b using a syringe or the like. A setting section 19 is provided on the rear end side of the upper surface of the handpiece 10 from the center. The setting section 19 has a display section 19a (see FIG. 2) that displays the current spray setting, a switching button 19b (see FIG. 2) that switches the spray setting of the spray liquid, an operation button 19c (see FIG. 2) that switches between starting and stopping the spray of the spray liquid, and a control section 19d that executes various controls. A spray amount adjustment dial 18 is provided on the right side of handpiece 10 (front of FIG. 1) for adjusting the amount of spray liquid per spray, and a manual spray button 16 is provided on the left side of handpiece 10 (rear of FIG. 1) for pressing the spray setting for manual spraying to perform one spray.

[0019] The conduit 20 has a pipe 20a (see FIG. 2) for supplying gas (air) to the handpiece 10 and an electric wire 20b (see FIG. 2) for supplying power to the handpiece 10 inside. The conduit 20 can be bent freely, so that the practitioner can freely move the handpiece 10 for treatment while leaving the main body 30 in place.

[0020] The main body 30 has an operation start button 30a on the front for switching between starting and stopping operation, and a power cord (not shown) on the back for connecting to a household power source, and one end of the conduit 20 is connected to the side.

[0021] FIG. 2 is a block diagram showing the configuration of the multiple component skin penetration system 1. Inside the handpiece 10, a spray tip 12 is provided on the tip side, and a transparent, approximately cylindrical spray cover 11 is attached to the outer periphery of this spray tip 12. This spray cover 11 is cylindrical, with the tip cut perpendicular to the axis of the cylinder, and two gas release spaces 11a are provided at the tip, one on the left and one on the right, facing each other. That is, the tip is mostly a circular ring-shaped tip on a plane, and at least a part of it is a recess that becomes the gas release space 11a. In the illustrated example, this gas release space 11a is formed by a recessed cutout groove that is partially switched at the end that is the open part of the spray cover 11.

[0022] A spray nozzle 12a for spraying the liquid to be sprayed is provided at the center of the spray tip 12, which is the tip of the handpiece 10, and a gas discharge section 14 is provided at the back of the spray nozzle 12a. A spray gas chamber 14b is provided inside the gas discharge section 14, and this spray gas chamber 14b is connected to the spray nozzle 12a. The air port 14a at the tip of the spray gas chamber 14b and the spray nozzle 12a are provided opposite each other. A spray liquid chamber 13 is provided on the outer periphery between the air port 14a and the spray nozzle 12a, and this spray liquid chamber 13 is connected to the spray nozzle 12a. The spray liquid chamber 13 is a sterile room and is connected to a liquid storage section 15a in which the liquid to be sprayed is stored. In addition, the discharge speed of the liquid to be sprayed and the gas discharged from the gas discharge section 14 through the spray nozzle 12a can be 100 km / h or more, preferably 500 km / h or more, and preferably 1000 km / h or more. In addition, the upper limit of this discharge speed can be 5000 km / h or less, preferably 3000 km / h or less, and preferably 2000 km / h or less. By discharging the compressed gas at high speed in a short period of time in this way, the liquid to be sprayed can be nanoized and discharged. By discharging (applying) the liquid to be sprayed in this nanoized form, the liquid to be sprayed can be penetrated deeper and more into the skin.

[0023] The outer periphery and the spray tip side of the spray tip 12 having the spray nozzle 12a are covered by the spray cover 11. Therefore, the spray liquid and gas sprayed from the spray nozzle 12a are confined within the spray cover 11 and are sprayed (ejected) vigorously onto the skin to be sprayed, and excess gas (including the spray liquid) is discharged to the outside through the gas release space 11a.

[0024] A solenoid 17 is provided in the handpiece 10 at the rear of the gas discharge unit 14. This solenoid 17 is provided midway through a pipe 20a that supplies gas to the spray gas chamber 14b of the gas discharge unit 14, and switches between passing and blocking the gas through the pipe 20a. This solenoid 17 and the control unit 19d that controls it function as a spray control unit.

[0025] In addition, inside the handpiece 10, a control unit 19d (see FIG. 1) is provided. In response to the operation of the switching button 19b, the control unit 19d switches the spray setting of the spray liquid to one of the following: manual spraying, which executes spraying once when the manual spray button 16 is pressed; first intermittent spraying, which performs instantaneous short-time spraying (e.g., 0.1 seconds, etc.) continuously at a first time interval (e.g., 1.5 seconds); second intermittent spraying, which performs short-time spraying (e.g., 0.1 seconds, etc.) continuously at a second time interval (e.g., 1.0 seconds interval) shorter than the first intermittent spray; and third intermittent spraying, which performs short-time spraying (e.g., 0.1 seconds, etc.) continuously at a third time interval (e.g., 0.5 seconds interval) even shorter than the second intermittent spray. The instantaneous short-time spray can be spraying for 1 second or less, preferably spraying for 0.5 seconds or less, more preferably spraying for 0.3 seconds or less, even more preferably spraying for 0.2 seconds or less, and preferably spraying for 0.1 seconds or less. The lower limit of the instantaneous short-term spray can be 0.001 seconds or more, preferably 0.01 seconds or more, and more preferably 0.05 seconds or more.

[0026] In addition, when the manual spray button 16 is pressed when the spray setting is manual spray (or when the time comes to spray with the first intermittent spray, second intermittent spray, or third intermittent spray), the control unit 19d executes spray control by turning on the solenoid 17 for a momentary predetermined time (for example, 0.1 seconds) during which spraying is performed, opening the pipe 20a to allow gas to pass, and then turning off the solenoid 17 to close the pipe 20a to prevent gas from passing. By controlling spraying in this way, the moment the solenoid 17 is turned off, negative pressure is generated in the spray liquid chamber 13, and the liquid to be sprayed in the liquid storage unit 15a is supplied to the spray liquid chamber 13, and the liquid to be sprayed together with the gas is sprayed from the spray nozzle 12a due to the instantaneous supply of gas when the solenoid 17 is next turned on.

[0027] The control unit 19d also executes an operation of displaying the current spray setting, and an error code, etc., in the event of an error, on the display unit 19a. Moreover, the control unit 19d executes these operations after the operation button 19c is turned on, and stops each operation when the operation button 19c is turned off. When the solenoid 17 is in the OFF state, the gas supplied from the pipe 20a flows into an exhaust path not shown.

[0028] The manual spray button 16, the solenoid 17, the display unit 19a, the switching button 19b, the operation button 19c, and the control unit 19d are supplied with power from the main body 30 via an electric wire 20b.

[0029] A spray amount adjustment unit 18 is provided downstream of the solenoid 17 in the handpiece 20. This spray amount adjustment unit 18 acts on the pipe 20a to adjust the amount of gas supplied when the solenoid 17 is turned on. This makes it possible to adjust the amount of gas and liquid to be sprayed that are ejected in one spray. The spray amount adjustment unit 18 may be configured to adjust the amount of spray by changing the opening size of the spray nozzle 12a. In this case, the spray range and spray intensity can also be adjusted.

[0030] The main body 30 is provided with a control unit 31, a power supply unit 32, and a gas supply unit 33. The control unit 31 controls the supply of gas by the gas supply unit 33. Specifically, when the operation start button 30a (see FIG. 1) is pressed to turn the power ON, the gas supply unit 33 is driven to start the supply of gas and starts the supply of power from the electric wire 20b to the unit inside the handpiece 10, and when the operation start button 30a (see FIG. 1) is pressed again to turn the power OFF, the supply of gas and power is stopped.

[0031] A power supply unit 32 supplies power to each unit. While the power is ON, the gas supply unit 33 drives an internal pump to continue to send gas through the pipe 20a to the nebulizing gas chamber 14b of the handpiece 10. In this embodiment, ambient air is sent as the gas, but it is possible to supply an appropriate gas such as oxygen, hydrogen, or an appropriate mixed gas by attaching a gas cylinder (not shown).

[0032] FIG. 3 is a flow chart illustrating the operation of the multiple component skin penetration system 1. When the operation start button 30a (see FIG. 1) is pressed to turn on the power, the control unit 19d receives the first liquid to be sprayed from the liquid inlet hole 15b (step S1). The received first liquid to be sprayed is supplied from the liquid storage unit 15a to the liquid chamber 13.

[0033] The control unit 19d accepts switching of the spray setting by the switching button 19b (step S2).

[0034] When the operating button 19c is pressed, the control unit 19d performs a first spray liquid spraying step of performing spraying according to the spray setting (step S3). That is, if the spray setting is manual spraying, spraying is performed at the timing when the manual spray button 16 is pressed, if the spray setting is the first intermittent spray, intermittent spraying at the first time interval is automatically and continuously performed, if the spray setting is the second intermittent spray, intermittent spraying at the second time interval is automatically and continuously performed, and if the spray setting is the third intermittent spray, intermittent spraying at the third time interval is automatically and continuously performed.

[0035] When the spray setting is changed (step S4: YES), the control unit 19d accepts the change in the spray setting (step S2), and executes spraying with the changed spray setting (step S3).

[0036] When spraying this spray liquid (first spray liquid, second spray liquid, etc.), the tip of spray cover 11 is brought into contact with the skin to perform spraying. When the circular ring-shaped tip of spray cover 11 is brought into full contact with the skin to perform spraying, excess gas is released from gas release space 11a at the tip together with the spray liquid. For this reason, most of the fine mist of spray liquid penetrates into the skin due to the force of release, and excess gas is released from gas release space 11a to prevent the skin from being pressed too hard by the gas pressure.

[0037] In other words, if the gas release space 11a does not exist and the entire circumference of the tip of the spray cover 11 is in contact with the skin when spraying, the released gas and spray liquid will press against the skin, and the amount of gas in the spray cover 11 will cause the skin to remain depressed. This will cause extra pressure on the skin, which may actually slow down the blood flow and hinder the penetration of the spray liquid. In contrast, by releasing the extra gas from the gas release space 11a, the skin will not be continuously pressured, and the skin will return to its original normal state immediately after the gas and spray liquid are sprayed onto the skin. Therefore, the permeated spray liquid can dissolve into the blood flow and lymph flow without stress, and a light and moderate stimulation is given to the skin, promoting the blood flow and lymph flow, allowing the permeated spray liquid to penetrate deeper.

[0038] If the process is not terminated even after the spraying of the first spray liquid is completed (step S5: NO), a second spray liquid spraying process is performed to receive the second spray liquid (step S6). The received second spray liquid is supplied from the liquid storage section 15a to the spray liquid chamber 13. After this, the process from step S2 onwards is repeated, and the second spray liquid is sprayed.

[0039] The above configuration and operation allow the active ingredient to be sprayed in a fine mist. Since only a small amount of spray liquid is used in one spray (instantaneous spray for 0.1 seconds or the like), even valuable and expensive active ingredients can be sprayed in small amounts many times, so that the active ingredient can be sprayed on the entire face by spraying one part at a time in sequence, and it is also possible to spray the neck and décolleté. The active ingredient can also be sprayed on the head, and the entire head can be sprayed, or multiple sprays can be applied to parts of the head where intensive penetration is desired.

[0040] When human stem cell culture fluid is used as the penetrating agent (first penetrating agent or second penetrating agent), spraying it on the skin of the face, neck, décolleté, etc. can increase firmness, provide a lifting effect, open the eyes, reduce wrinkles, slim the contours, make the skin look fresh and rejuvenated, etc. In particular, when human umbilical cord stem cell culture supernatant is used, these effects are more pronounced.

[0041] In addition, when human stem cell culture fluid is sprayed on a wounded or burned area of ​​the skin, the wound or burn will heal faster than usual, and will leave a clean wound with no scars even after healing. These effects are particularly pronounced when human umbilical cord stem cell culture supernatant is used.

[0042] Furthermore, when human stem cell culture fluid is used as the penetrating agent (first penetrating agent or second penetrating agent), spraying it on the head can produce hair growth effects and youthful looking effects, such as hair growth, hair volume increase, hair line advancement, hair firmness, hair strength, thickening, and turning gray hair back to black. In particular, when human umbilical cord stem cell culture supernatant is used, these effects are more pronounced.

[0043] In addition, when NMN is used as the penetrating agent (first penetrating agent or second penetrating agent), spraying it on the skin of the face, neck, décolleté, etc. can increase firmness, have a lifting effect, open the eyes, reduce wrinkles, slim the contours, and make the skin look fresh and youthful.

[0044] In addition, when NMN is sprayed on injured or burned areas of the skin, the wound or burn will heal faster than usual, and will leave a clean wound with no scars even after healing.

[0045] In addition, when NMN is used as the penetrating agent (first penetrating agent or second penetrating agent), spraying it on the head can have the following effects: hair growth, increased hair volume, advancement of the hairline, increased hair firmness, stronger hair strength, thicker hair, and turning gray hair back to black.

[0046] Furthermore, when human stem cell culture medium is used as one of the first and second penetrating agents, and NMN is used as the other, the above-mentioned effects can be obtained more significantly than when either agent is used alone, and even greater effects can be obtained by combining the two rather than using twice the amount of one agent during treatment.

[0047] In particular, when the first spray solution using the first penetrating agent and the second spray solution using the second penetrating agent are sprayed separately and in sequence, the active ingredients can be permeated into the skin one by one, and each is permeated in turn by the bloodstream and lymphatic flow, resulting in a synergistic effect. In particular, when this treatment is performed on the face, it can produce a remarkable effect in changing the contours and size of the eyes, and some people may even suspect that they have had cosmetic surgery.

[0048] In addition, since the first spray liquid and the second spray liquid are sprayed by the spray device 5, a fixed amount of spray can be sprayed with a constant intensity each time. Therefore, the same effect can be obtained regardless of the skill of the practitioner, as long as the practitioner sprays the same area in the same order. EXAMPLES

[0049] An embodiment of the multiple component skin penetration method using the multiple component skin penetration system 1 described above will be described below. The first penetrating agent 2, the second penetrating agent 3, and the adjusting agent 4 used in this example are as follows.

[0050] First penetrating agent 2: NMN <Details> β-Nicotinamide Mononucleotide - Grain size: 16 mesh or less ·Bulk specific gravity: 0.6g / ml or more ·Purity 99% or more (composition ratio: 100%) Processing: Micro granulation processing ·Shape: white crystalline powder ·Amount: 500mg Amount used in this example: 1 / 3 (=166.67 mg)

[0051] Second permeation agent 3: Human umbilical cord stem cell culture supernatant <Details> Human mesenchymal stem cells derived from umbilical cord matrix (hMSC-UC) · Proliferative cell culture Passage 2 (third culture) ·Storage method: Cryopreservation ·Amount: 1ml Amount of data in this example: All

[0052] Adjustment agent 4: Purified water - 400,000 times purer than tap water ·Amount: 400ml Amount used in this example: (1) 3 ml is used to mix with 166.67 g of NMN powder as the first penetrant 2. After mixing, about 3.17 ml of the second spray liquid is left. (2) 2 ml is used to mix with 1 ml of human umbilical cord stem cell culture supernatant as the second penetrating agent 3. After mixing, 3 ml of the first spray liquid is added.

[0053] 0. Preparation First, the subjects' skin was pre-cleansed and then massaged to drain lymph nodes on the neck, décolleté, and face as a preliminary step.

[0054] 1. Spraying the first spray liquid (NMN) The first liquid for spraying was sprayed onto the décolleté, neck, and face using the spray device 5. Spraying was performed on each area in turn with the tip of the spray cover 11 in contact with the skin. Spraying was performed approximately 1,000 times in total.

[0055] 2. Spraying of the second liquid for spraying (human umbilical cord stem cell culture supernatant) The second liquid for spraying was sprayed onto the décolleté, neck, and face using the spray device 5. Spraying was performed on each area in turn with the tip of the spray cover 11 in contact with the skin. Spraying was performed approximately 1,000 times in total.

[0056] The above treatment was performed on two patients, and the following results were obtained:

[0057] [Table 1]

[0058] In particular, because the change in the contour is easy to see, an example of the change in subject 1 will be described in Figure 4. Figure 4(A) is a photograph of the stage where the above-mentioned "0. Preparation" was completed, that is, the stage where the lymphatic drainage massage was finished. Figure 4(B) is a photograph of the condition after spraying NMN and then spraying human umbilical cord stem cell culture supernatant. FIG. 4(C) is a photograph showing the facial contour of FIG. 4(A) before spraying superimposed on the facial contour of FIG. 4(B) after spraying. As shown in this figure, from the state after lymphatic drainage (Before) to the state after (After), it is clear that significant effects can be obtained by simply performing this treatment once, in which NMN and human stem cell culture medium are applied in sequence.

[0059] Furthermore, the subject was told by a friend the next day that she must have gone for surgery, and a person she met a week after the procedure also said she must have gone for surgery. In other words, this procedure, which involves applying NMN and human stem cell culture fluid in sequence, once can produce significant results, and these results are sustained. EXAMPLES

[0060] In Example 2, only the spraying order was changed from Example 1. That is, the first penetrating agent 2 and the second penetrating agent 3 were exchanged as follows. (1) 2 ml is used to mix with 1 ml of human umbilical cord stem cell culture supernatant as the first penetrating agent 2. After mixing, 3 ml of the first spray liquid is used. (2) 3 ml is used to mix with 166.67 g of NMN powder as the second penetrant 3. After mixing, about 3.17 ml of the second spray liquid is left. Other than this, the procedure was the same as in Example 1, with massage, spraying of the first spray liquid, and spraying of the second spray liquid being carried out in that order. The above treatment was performed on two patients, and the following results were obtained:

[0061] [Table 2]

[0062] In addition, because subject 3 had atopic dermatitis, the first spray liquid (human umbilical cord stem cell culture supernatant) and the second spray liquid (NMN) were sprayed on the atopic areas as well. As a result, the symptoms of the atopic dermatitis improved, and the skin in the atopic areas became more moisturized, firmer, and shinier. EXAMPLES

[0063] Next, the same formulation and treatment as in Example 1 were used to spray the scalp, and the hair growth and regrowth effect was confirmed. At this time, the preparatory massage included a massage of the back of the neck and the scalp. The other spray liquids and treatment order were the same as in Example 1. Each treatment was counted as one session, and the effects were measured after a total of four treatments, with intervals ranging from one week to one month between each treatment. After performing the treatment on two people, both showed clear hair growth results. EXAMPLES

[0064] Next, the same formulation and treatment as in Example 2 were used to spray the scalp, and the hair growth and regrowth effect was confirmed. At this time, the back of the neck and scalp were massaged in advance as a preparatory massage. The other spray liquids and treatment order were the same as in Example 2. Each treatment was counted as one session, and the effects were measured after a total of four treatments, with intervals ranging from one week to one month between each treatment. After performing the treatment on two people, both showed clear hair growth results.

[0065] The present invention is not limited to the above-described embodiment, but may be embodied in various other forms.

[0066] For example, before spraying the first liquid to be sprayed, holes may be made on the surface of the skin with a roller having a plurality of fine needles of a predetermined length arranged on the outer circumference of a cylinder, and then the first liquid to be sprayed and the second liquid to be sprayed may be sprayed in succession. In this case, the penetration of the liquid to be sprayed into the skin can be improved, and a better effect can be obtained. In this case, it is preferable that the needles are made of titanium. The predetermined length can be 0.1 mm to 1 mm, preferably 0.1 to 0.5 mm, and preferably 0.25 mm.

[0067] In addition, by performing a lymphatic massage as a preliminary step, blood flow and lymph flow can be improved, resulting in better results (massage effect + spray effect), but spraying of the first spray liquid and the second spray liquid may be performed without such preliminary step. Even in this case, a clear change can be seen before and after spraying.

[0068] In addition, the first liquid for spraying is sprayed 1,000 times, and the second liquid for spraying is sprayed 1,000 times, but this is not limited to this, and each can be sprayed 500 times, 300 times, or conversely increased to 1500 times, etc., and can be an appropriate number of times. The number of sprays depends on the area of ​​the skin to be sprayed, but can be 10 times or more for one type of spray liquid, preferably 50 times or more, more preferably 100 times or more, even more preferably 300 times or more, and preferably 500 times or more. In addition, the upper limit of the number of sprays can be 3000 times or less for one type of spray liquid, preferably 2000 times or less, more preferably 1500 times or less, and preferably 1000 times or less.

[0069] The area covered by the inside of the tip of the circular spray cover 11 can be applied to 40% or more of the skin area to be treated, preferably 50% or more, more preferably 60% or more, even more preferably 70% or more, more preferably 80% or more, and preferably 90% or more. Although spraying the same position once is sufficient, more active ingredients may be permeated by spraying two or more times. In particular, it is preferable to spray two or more times partially on areas where intensive care is desired, such as fine lines around the eyes, nasolabial folds, and wrinkles on the forehead. This increases the penetration of the active ingredients into the areas where intensive care is desired, and more preferable effects can be obtained in the areas where intensive care is desired.

[0070] In addition, for a spray liquid using a first penetrant 2 and a second penetrant 3, either of the following methods can be used: the first penetrant 2 and the second penetrant 3 are mixed with an adjuster 4 and then poured into the liquid storage section 15, thereby feeding all of the components simultaneously; or the first penetrant 2 and the adjuster 4 are mixed and fed into the liquid storage section 15 as the first spray liquid and sprayed, and then within a specified time, the second penetrant 3 and the adjuster 4 are mixed and fed into the liquid storage section 15 as the second spray liquid, thereby feeding the components sequentially.

[0071] In the sequential addition of each component, the predetermined time from the completion of spraying of the first spray liquid to the start of spraying of the second spray liquid can be within one week, preferably within 48 hours, preferably within 24 hours, more preferably within 12 hours, even more preferably within 6 hours, even more preferably within 3 hours, even more preferably within 1 hour, even more preferably within 30 minutes, and preferably within 10 minutes. By adding the components sequentially in this manner, the components of the first penetrant 2 are sufficiently permeated, and the components of the second penetrant 3 are also sufficiently permeated, and the effects of both components can be obtained simultaneously, thereby exerting a synergistic effect. In particular, by shortening the predetermined time from the spraying of the first spray liquid (first penetrant 2 + regulator 4) to the spraying of the second spray liquid (second penetrant 3 + regulator 4), the synergistic effect of both components can be enhanced. In addition, by adding them sequentially, each component can be thoroughly permeated rather than being mixed and added at the same time.

[0072] When all ingredients are added at the same time, the overall spray time can be shortened compared to sequential addition of ingredients. For example, when ingredients are added sequentially, it is necessary to spray the first spray liquid 1000 times on each part of the face (and the neck and décolleté may be added), and then spray the second spray liquid again 1000 times on each part of the face (and the neck and décolleté may be added), which takes the time to spray a total of 2000 times, but when all ingredients are added at the same time, it is possible to spray each part of the face (and the neck and décolleté may be added) only 1000 times in total, which shortens the time to half.

[0073] In the case of simultaneous injection of all the components, a third penetrant (a third active ingredient) may be mixed in addition to the first penetrant 2 and the second penetrant 3, and then all the components may be injected simultaneously and sprayed. The third penetrant may be any suitable substance, for example, a cannabinoid (third active ingredient) such as CBD (cannabidiol), CBN (cannabinol) or CBG (cannabigenol), and in the case of this cannabinoid, it is preferable that THC (tetrahydrocannabinol) has been removed.

[0074] The third penetrating agent can also be a proteoglycan. In this case, by applying it to the scalp in particular, it can improve the effects on hair (hair growth effect, hair volume increase effect, hair line advancement effect, hair firmness increase effect, hair strength increase effect, hair thickening effect, gray hair turning back to black hair, etc.).

[0075] In the case of sequential addition of components, after spraying the first spray liquid (first penetrant 2 + regulator 4) and the second spray liquid (second penetrant 3 + regulator 4), the third spray liquid (third penetrant + regulator 4) can be sprayed within a predetermined time. Furthermore, it is also possible to add the third spray liquid (fourth penetrant + regulator 4) by spraying it within a predetermined time.

[0076] In addition, the first active ingredient is NMN, and the second active ingredient is human stem cell culture fluid, which are applied (sprayed) in that order, but the order can be reversed, with the first active ingredient being human stem cell culture fluid and the second active ingredient being NMN, which are applied (sprayed) in that order. In this case, too, each active ingredient can be penetrated sequentially to exert their respective synergistic effects.

[0077] In addition, one of the first and second active ingredients may be a human stem cell culture medium, and the other may be a proteoglycan, and applied (sprayed) in that order. In this case, too, the effects of each ingredient can be fully exerted, resulting in a synergistic effect.

[0078] In addition, the method of application is preferably spraying as described above, but is not limited to this, and other methods such as applying to the skin by hand, soaking in a pack or the like and placing it on the skin, etc. can also be used. In this case, the penetration of the active ingredient can be increased by making multiple small holes in the skin using a fine needle. In this case, a certain degree of effect can also be obtained, and by applying the active ingredients in order, the synergistic effect of each active ingredient can be exerted. [Industrial Applicability]

[0079] The present invention can be used in industries such as beauty salons and hair growth salons. [Explanation of symbols]

[0080] 1. Multiple-ingredient skin penetration system 12a…Spray nozzle 15a...Liquid storage section 17...Solenoid 19d…Control section 33...Gas supply section

Claims

1. A step of applying a first composition containing one or more selected from a human stem cell culture medium, NMN, and proteoglycan; and applying a second composition containing one or more components other than the first composition selected from a human stem cell culture medium, NMN, and proteoglycan. Skin beauty treatments (excluding medical procedures).

2. One of the first composition and the second composition contains a human stem cell culture medium, and the other contains NMN. The skin cosmetic method according to claim 1 (excluding medical procedures).

3. After the massage, applying the first composition; Carrying out the step of applying the second composition The skin beauty method according to claim 2 (excluding medical procedures).

4. A step of applying a first composition containing one or more selected from a human stem cell culture medium, NMN, and proteoglycan; and applying a second composition containing one or more components other than the first composition selected from a human stem cell culture medium, NMN, and proteoglycan. Hair growth and regrowth methods.

5. A liquid storage section that stores a liquid to be sprayed containing an active ingredient; a spray nozzle for spraying the spray liquid contained in the liquid container; A gas supply unit that supplies a gas; a spray control unit that instantly supplies gas supplied from the gas supply unit to the spray nozzle to spray the spray liquid together with the gas from the spray nozzle, The first active ingredient is a human stem cell culture medium, NMN, or proteoglycan; the second active ingredient is a human stem cell culture medium, NMN, or proteoglycan, which is different from the first active ingredient; A first penetrant containing a first active ingredient for preparing the liquid for spraying and a second penetrant containing a second active ingredient different from the first active ingredient for preparing the liquid for spraying are adjusted with an adjuster, respectively or together, to form a liquid for spraying. Multiple ingredient skin penetration method.

6. a first spray liquid spraying step of spraying a first spray liquid in which the first penetrant is adjusted with the adjuster; a second spray liquid spraying step of spraying a second spray liquid in which the second penetrant is adjusted with the adjusting agent; Run sequentially instead of simultaneously The method of claim 5 for penetrating the skin with a multiple component composition.

7. One of the first and second osmotic agents is a human stem cell culture medium, and the other is NMN. The method of claim 6, wherein the multiple components are applied to the skin.

8. The time period from the completion of the first spray liquid spraying step to the start of the second spray liquid spraying step is within one week. The method of claim 7 for penetrating the skin with a multiple component composition.

9. A liquid storage section that stores a liquid to be sprayed containing an active ingredient; a spray nozzle for spraying the spray liquid contained in the liquid container; A gas supply unit that supplies a gas; a spray control unit that instantaneously supplies the gas supplied from the gas supply unit to the spray nozzle to spray the spray liquid together with the gas from the spray nozzle, A first penetrant containing a first active ingredient to prepare the spray liquid; A second penetrant containing a second active ingredient different from the first active ingredient for forming the spray liquid; and an adjuster for adjusting the first penetrant and / or the second penetrant, The first active ingredient is a human stem cell culture medium, NMN, or proteoglycan; The second active ingredient is a human stem cell culture medium, NMN, or proteoglycan, which is different from the first active ingredient. Multi-ingredient skin penetration system.

10. A spray cover is provided to cover the outer periphery and the spray tip side of the spray nozzle, The spray cover has a most part of the outer periphery of the open portion at the tip side formed on a flat surface, and at least a part of the outer periphery is formed with a concave gas release space.

10. The multiple component skin penetrating system of claim 9.

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