Virus inactivation agent composition

Abstract

To provide a virus inactivation agent composition that is highly safe, that has a high virus inactivation effect, and that enables reduction in the added amount of ethanol, which is a virus inactivation component.SOLUTION: Provided is a virus inactivation agent composition, which is an acidic aqueous solution obtained by mixing (A) 10 to 60 mass% of ethanol as a virus inactivation component, (B) fumaric acid as a virus inactivation efficacy enhancement component, and (C) water, wherein the content of the virus inactivation efficacy enhancement component is 0.05 mass% or more.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a virus inactivating composition comprising a virus inactivating component formulated in an aqueous solution. [Background technology]

[0002] Food poisoning caused by norovirus of the Calicivirus family has become a problem in food manufacturing and cooking facilities, medical facilities, educational facilities, etc. Known methods for inactivating norovirus include a method using sodium hypochlorite solution and a method using hot water disinfection. However, disinfection using hypochlorite is inconvenient because it bleaches the treated surface and generates a foul odor, and is not safe enough because it generates chlorine gas when mixed with acidic bleach. In addition, in hot water disinfection, the temperature drops due to contact with the object to be disinfected, making it difficult to maintain the desired temperature. Furthermore, there is a risk of burns from hot water. In light of this situation, there is a demand for a virus inactivator that is highly safe and has a high virus inactivation effect.

[0003] In order to inactivate such viruses, virus inactivating agents containing lower alcohols as virus inactivating components are widely used. For example, Patent Document 1 discloses a viricide containing at least 70% by weight of ethanol and / or propanol and 0.5 to 5% by weight of a short-chain organic acid.

[0004] Furthermore, Patent Document 2 discloses a method for inactivating non-enveloped virus particles by contacting the virus particles with an alcohol composition containing an effect-enhancing amount of one or more enhancers selected from the group consisting of a C1-6 alcohol, and a cationic oligomer or polymer, a proton donor, a chaotropic agent, and a mixture thereof, and wherein when the alcohol composition contains a proton donor, the alcohol composition further contains a synergistic amount of a cationic oligomer or polymer, and also discloses that the alcohol concentration is 50% by mass or more.

[0005] Patent Document 3 discloses a disinfectant characterized by containing (A) 50 to 70% by weight of ethanol and (B) 0.05 to 4.50% by weight of at least one selected from the group consisting of organic acids, organic acid salts, and ethanolamines, and having a pH of 6 to 12. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] Japanese Patent Publication No. 14702 / 1983 [Patent Document 2] Special Publication No. 2009-526060 [Patent Document 3] JP 2016-210807 A Summary of the Invention [Problem to be solved by the invention]

[0007] However, the virus inactivators of Patent Documents 1 to 3 all have a relatively high alcohol concentration, and have problems with flammability and alcohol irritation. Therefore, caution is required when using them in places where fire is used, such as around the kitchen. In addition, there is a risk of paint or wax peeling off when a disinfectant containing ethanol is applied to a resin component, and there is also a problem that it is difficult to use for people who do not like the smell of ethanol or whose hands become rough from ethanol. In addition, the pH of Patent Document 3 is neutral to alkaline, ranging from 6 to 12, so there is a problem of corrosion due to alkali. Furthermore, since food and tableware are present around the kitchen, there has been a demand for a highly safe virus inactivator that does not affect health even if it adheres to them.

[0008] In view of the above problems, an object of the present invention is to provide a virus inactivator composition which enables a reduction in the amount of ethanol, which is a virus inactivating component, and which has a high virus inactivation effect and is also excellent in safety. [Means for solving the problem]

[0009] In order to achieve the above object, the present invention provides a virus inactivator composition comprising (A) 10% by mass to 60% by mass of ethanol as a virus inactivation component, (B) fumaric acid as a virus inactivation efficacy enhancing component, mixed with (C) water to form an acidic aqueous solution, wherein the blending amount of the virus inactivation efficacy enhancing component is 0.05% by mass or more.

[0010] The present invention also relates to a virus inactivator composition comprising (A) 10% by mass to 60% by mass of ethanol as a virus inactivating component, and (B) one or more components selected from phosphoric acid and citric acid as a virus inactivation efficacy enhancing component, mixed with (C) water to form an acidic aqueous solution, the content of the virus inactivation efficacy enhancing component being 0.05% by mass or more and 0.5% by mass or less (however, when the composition contains citric acid and an extract of a plant of the genus Diospyros containing tannin, the content of the virus inactivating efficacy enhancing component is 0.05% by mass or more and 0.5% by mass or less). the fatty acid constituting the composition is caprylic acid, the composition contains glycerol fatty acid ester and grapefruit seed extract (obtained from grapefruit seeds and mainly composed of fatty acids and flavonoids), the composition contains phloroglucinol polymer which is a compound formed by polymerizing seven or more molecules of phloroglucinol, the composition contains lactic acid, citric acid and a zinc-containing compound which liberates zinc ions in solution, the composition contains PEG / PPG-20-35 / 5-30 dimethicone, and the composition contains an alcohol compound having 10 to 40 carbon atoms).

[0011] Furthermore, the present invention is characterized in that the virus inactivating composition having the above-mentioned configuration contains 30% by mass to 60% by mass of ethanol as the virus inactivating ingredient.

[0012] Furthermore, the present invention is characterized in that in the virus inactivating composition having the above-mentioned configuration, the virus is a non-enveloped virus. Effect of the Invention

[0013] According to the first aspect of the present invention, 10% to 60% by mass of ethanol (A) as a virus inactivating component, and (B) fumaric acid as a virus inactivation efficacy enhancing component are mixed with (C) water to form an acidic aqueous solution, and the amount of the virus inactivation efficacy enhancing component is set to 0.05% by mass or more, thereby providing a virus inactivating composition with a low ethanol concentration. Therefore, the virus inactivating composition is free from flammability and irritation to the skin caused by ethanol and alkali, has an excellent feel when used, and has high virus inactivation efficacy.

[0014] According to the second aspect of the present invention, a virus inactivator composition having a low ethanol concentration is obtained by mixing (A) 10% to 60% by mass of ethanol as a virus inactivating component, and (B) one or more selected from phosphoric acid and citric acid as a virus inactivation efficacy enhancing component with (C) water to prepare an acidic aqueous solution, and setting the blending amount of the virus inactivation efficacy enhancing component to 0.05% to 0.5% by mass. Therefore, the virus inactivator composition is free from flammability and irritation to the skin caused by ethanol and alkali, has an excellent feel when used, and has high virus inactivation efficacy.

[0015] According to the third aspect of the present invention, by setting the blending amount of ethanol, which is a virus inactivating component, to 30% by mass to 60% by mass in the virus inactivating composition of the first or second aspect, the virus inactivating effect of the virus inactivating composition is further enhanced.

[0016] According to the fourth aspect of the present invention, by inactivating a non-enveloped virus in the virus inactivator composition of the second aspect, the virus inactivator composition has an excellent inactivation effect against non-enveloped viruses that have low drug susceptibility and are difficult to inactivate. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0017] The virus inactivator composition of the present invention will be described in detail below. The virus inactivator composition of the present invention is prepared by mixing (A) 10% by mass to 60% by mass of ethanol as a virus inactivating component, (B) one or more specific organic acids or inorganic acids as a virus inactivation effect enhancing component, with (C) water to form an acidic aqueous solution.

[0018] It has been known that ethanol (A), which is blended as a virus inactivating component in the virus inactivator composition of the present invention, has a high virus inactivating effect. However, the present inventors were the first to discover that the combined use of ethanol with a specific organic acid and / or inorganic acid described below exhibits a synergistic virus inactivating effect.

[0019] If the amount of ethanol in the virus inactivating composition of the present invention is too small, sufficient virus inactivation effect may not be obtained, whereas if the amount is too large, there are problems such as increased flammability and irritation.

[0020] In the virus inactivator composition of the present invention, as shown in the examples described later, in order to obtain sufficient virus inactivation efficacy and reduce flammability and irritation caused by ethanol, ethanol is blended in an amount of 10% by mass to 60% by mass based on the entire virus inactivator composition. In addition, blending ethanol in an amount of 10% by mass to 40% by mass based on the entire virus inactivator composition is preferable because it can further reduce the odor and irritation of ethanol. In addition, blending ethanol in an amount of 30% by mass to 60% by mass based on the entire virus inactivator composition is preferable because it can further enhance the virus inactivation effect.

[0021] Specific examples of organic acids to be blended as the (B) virus inactivation effect enhancing component in the virus inactivator composition of the present invention include fumaric acid and citric acid. Specific examples of inorganic acids include phosphoric acid. These organic acids and / or inorganic acids may be used alone or in combination of two or more.

[0022] Among the above organic acids and / or inorganic acids, by using fumaric acid and phosphoric acid in particular, the virus inactivation efficacy of ethanol, which is a virus inactivating ingredient, can be significantly improved even with a low blend amount.

[0023] The pH of the virus inactivator composition of the present invention is desirably 1 to 6. This makes the virus inactivator composition acidic to weakly acidic, less corrosive to the skin than alkaline virus inactivator compositions, and provides a virus inactivator composition with excellent usability.

[0024] The amount of the virus inactivation efficacy enhancing component in the virus inactivator composition of the present invention is not particularly limited. However, if the amount is too small, a sufficient enhancing effect on the virus inactivation efficacy of ethanol may not be obtained.

[0025] As shown in the examples described later, in order to obtain a sufficient virus inactivation efficacy enhancing effect, the organic acid and / or inorganic acid as the virus inactivation efficacy enhancing component is preferably blended in an amount of 0.05% by mass or more, more preferably 0.07% by mass or more, and even more preferably 0.1% by mass or more, relative to the entire virus inactivator composition. Also, the organic acid and / or inorganic acid as the virus inactivation efficacy enhancing component is preferably blended in an amount of 5% by mass or less, more preferably 2% by mass or less, and even more preferably 1% by mass or less, relative to the entire virus inactivator composition.

[0026] In the present invention, the mass ratio (A) / (B) of the virus inactivating component (A) to the virus inactivation effect enhancing component (B) is 1.30≦log 10 It is preferable that [(A) / (B)]≦3.10 is satisfied, and 1.50≦log 10 It is more preferable that [(A) / (B)]≦3.00 is satisfied, and 2.10≦log 10 It is more preferable that [(A) / (B)]≦2.90 is satisfied.

[0027] The virus inactivator composition of the present invention may contain a surfactant as needed. For example, a surfactant has a foam-generating property (foaming property), and the use of a surfactant with excellent foaming property can suppress dripping when the virus inactivator composition of the present invention is sprayed onto a wall surface with a trigger spray or the like, and also makes it easier to visually confirm the applied area.

[0028] As the surfactant to be blended in the virus inactivator composition of the present invention, any of anionic surfactants, cationic surfactants, nonionic surfactants, and amphoteric surfactants can be suitably used. Among these, cationic surfactants having antiviral activity in addition to surface activity can be more suitably used. The amount of surfactant blended in the virus inactivator composition of the present invention is not particularly limited, but is preferably 0.1% by mass or more and 10% by mass or less.

[0029] Examples of anionic surfactants include fatty acid soaps, alkylbenzene sulfonates, linear alkylbenzene sulfonates, alkyl sulfates, α-olefin sulfonates, alkyl phosphates, polyoxyethylene alkyl ether sulfates, polyoxyethylene alkyl phenyl ether sulfates, and polyoxyethylene alkyl ether phosphates.

[0030] Examples of cationic surfactants include quaternary ammonium salts such as lauryltrimethylammonium chloride, benzalkonium chloride, benzethonium chloride, didecyldimethylammonium chloride, and chlorhexidine gluconate.

[0031] Examples of nonionic surfactants include polyoxyethylene alkyl ethers, polyoxyethylene alkylphenyl ethers, polyoxyethylene higher fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, coconut oil fatty acid diethanolamide, polyoxyethylene polyoxypropylene alkyl ethers, fatty acid alkanolamides, and alkylamine oxides.

[0032] Examples of amphoteric surfactants include betaine surfactants, such as lauryl-N,N-dimethylacetate betaine, lauryl amidopropyl-N,N-dimethylacetate betaine, and coconut alkyl amidopropyl-N,N-dimethylhydroxypropyl sulfobetaine.

[0033] The virus inactivating composition of the present invention is an aqueous type, and water is mainly used as a solvent. Examples of water include purified water such as ion-exchanged water and reverse osmosis water, ordinary tap water, industrial water, deep sea water, etc.

[0034] Furthermore, the virus inactivator composition of the present invention may contain other components, such as inorganic antibacterial agents, organic antibacterial agents, virus inactivators, antialgal agents, antirust agents, solvents, chelating agents, fragrances, deodorizing components, and pH adjusters, as necessary, within a range that does not impair the effects of the present invention, thereby imparting antibacterial effects, virus inactivating effects, antialgal effects, antirust effects, cleaning effects, aromatic properties, deodorizing properties, and the like.

[0035] Examples of inorganic antibacterial agents, organic antibacterial agents, and virus inactivating agents include isopropylmethylphenol (IPMP), carvacrol, thymol, triclosan, methylparaben, ethylparaben, propylparaben, butylparaben, 4-chloro-3,5-dimethylphenol, orthophenylphenol, o-cresol, m-cresol, p-cresol, tebuconazole, enilconazole, grapefruit seed extract, persimmon seed extract, grape seed extract, monolaurin, monocaprin, monocaprylin, benzoic acid, sorbic acid, glycine, alkyldiethylaminoglycine, polylysine, dehydroacetic acid, chloramine, 3-iodo-2-propyl-N-butylcarbamate (IPBC), phenoxyethanol, silver zeolite, zinc pyrithione, thiamine lauryl sulfate, milt protein, hydroxyalkylchitosan, and chitosan.

[0036] An example of an anti-algae agent is sodium dichloroisocyanurate, etc. An example of an anti-rust agent is sodium benzoate, etc.

[0037] Examples of the solvent include hydrocarbon solvents such as normal paraffin, isoparaffin, liquid paraffin, naphthenic hydrocarbons, Vaseline, squalane, and α-olefin oligomers; alcohol solvents such as 1-propanol, 2-propanol (IPA), 1-butanol, 2-butanol, tertiary butanol, 1-pentanol, 1-hexanol, benzyl alcohol, and 2-phenylethanol; and glycol solvents such as 2-phenoxyethanol (ethylene glycol monophenyl ether), ethylene glycol, propylene glycol, 1-phenoxy-2-propanol (propylene glycol phenyl ether), 1,3-butylene glycol, propylene glycol monobutyl ether, dipropylene glycol monobutyl ether, and tripropylene glycol monobutyl ether.

[0038] Examples of fragrances include limonene such as d-limonene, pinene such as α-pinene and β-pinene, cymene such as p-cymene, hydrocarbon fragrances such as indene and caryophyllene, menthol such as linalool, geraniol, citronellol, and l-menthol, ethyl linalool, borneol, anise alcohol, β-phenethyl alcohol, p-menthane-3,8-diol, terpineol such as α-terpineol and γ-terpineol, 1-hexenol, cis-3-hexen-1-ol, tetrahydrogeraniol, santalinol, and cinnamyl alcohol. Alcohol-based fragrances such as cedrol, ether-based fragrances such as galaxolide, β-naphthyl methyl ether, cineole, ambroxide, p-cresil methyl ether, phenol-based fragrances such as anethole, eugenol, isoeugenol, vanillin, ethyl vanillin, octanal, nonanal, undecyl aldehyde, undecanal, decyl aldehyde, n-butyraldehyde, isobutyraldehyde, hexyl aldehyde, citral, citronellal, benzaldehyde, cinnamic aldehyde, anisaldehyde, cumin aldehyde, adoxaldehyde, Aldehyde fragrances such as sal, amyl cinnamic aldehyde, and cyclamen aldehyde; ketone fragrances such as musk ketone, carvone, menthone, camphor, acetophenone, butyrophenone, tonalide, α-ionone, β-ionone, α-methyl ionone, β-methyl ionone, α-isomethyl ionone, β-isomethyl ionone, γ-methyl ionone, γ-isomethyl ionone, damascone, α-damascone, β-damascone, acetyl cedrene, cashmeran, cis-jasmone, and dihydrojasmone; γ-butyrolactone, γ-nonalactone, γ- Lactone fragrances such as decalactone, γ-undecalactone, coumarin, cineole, amble redd, and jasmolactone, geranyl formate, octyl acetate, geranyl acetate, benzyl acetate, cinnamyl acetate, tetrahydrogeranyl acetate, menthyl acetate, linalyl acetate, butyl propionate, benzyl acetate, methyl benzoate, allyl hexanoate, allyl heptanoate, allyl cyclohexane propionate, allyl amyl glycolate, amyl valerianate, amyl salicylate, isoamyl acetate,Butyl acetate, ethyl butyrate, acetyl eugenol, isoamyl salicylate, allyl caproate, ethyl caproate, ethyl propionate, ethyl acetoacetate, methyl salicylate, citronellyl acetate, citronellyl formate, cinnamyl acetate, stearyl acetate, stearyl propionate, cedryl acetate, terpinyl acetate and other ester-based fragrances, amyl cinnamic aldehyde dimethyl acetate acetal fragrances such as citral dimethyl acetal, indole, geranyl nitrile, citronellyl nitrile, acetaldehyde phenylethyl propyl acetate, thesalon, auranthiol, linalool oxide, peppermint oil, orange oil, lemon oil, lavender oil, peppermint oil, eucalyptus oil, citronella oil, lime oil, yuzu oil, jasmine oil, cypress oil, green tea essential oil, neroli oil, geranium oil, petitgrain oil, lemongrass oil, cinnamon oil, lemon Eucalyptus Oil, Thyme Oil, Perilla Oil, Pine Oil, Rose Oil, Rosemary Oil, Camphor Oil, Aromatic Oil, Clary Sage Oil, Sandalwood Oil, Spearmint Oil, Star Anise Oil, Lavandin Oil, Oakmoss Oil, Ocotoia Oil, Patchouli Oil, Tonka Bean Tincture, Turpentine Oil, Wanilla Bean Tincture, Basil Oil, Nutmeg Oil, Clove Oil, Boer Rose Oil, Cananga Oil, Cardamom Oil, Cassia Oil, Cedarwood Oil, Mandarin Oil, Tangerine Oil, Anise Oil, Bay Oil, Coriander Oil oil, elemi oil, fennel oil, galbanum oil, hiba oil, vetiver oil, bergamot oil, ylang-ylang oil, grapefruit oil, abies oil, accion oil, almond oil, angelica root oil, peper oil, mint oil, perch oil, boerbo rose oil, kayabuchi oil, gananga oil, capsicum oil, caraway oil, celery oil, cognac oil, cumin oil, jill oil, estgolan oil, garlic oil, ginger oil, hops oil, sage oil, turpentine oil, etc.

[0039] Examples of deodorizing components include sugar cane extract, green tea extract, tea dry distillate, persimmon extract, grapefruit extract, moso bamboo extract, yuzu seed extract, and forsythia extract. From the viewpoint of promoting the norovirus inactivation effect in addition to the deodorizing effect, sugar cane extract is preferred.

[0040] Examples of pH adjusters include other organic acids such as acetic acid, malic acid, salicylic acid, other inorganic acids such as hydrochloric acid, sodium citrate, sodium carbonate, sodium bicarbonate, sodium hydroxide, and the like.

[0041] The virus inactivating composition of the present invention thus obtained can be applied or sprayed onto virus-contaminated areas such as areas touched by a virus-infected person, areas where vomit from a virus-infected person has been disposed of, and clothing. The virus inactivating composition of the present invention can be applied safely and simply by limiting the blending amount of flammable and irritating ethanol to 10 to 60 mass %, and is therefore highly practical.

[0042] Furthermore, the virus inactivating composition of the present invention has a high inactivation effect not only on enveloped viruses such as influenza virus, coronavirus, and herpes virus, but also on non-enveloped viruses such as norovirus, rotavirus, rhinovirus, and adenovirus, etc. Therefore, it can be suitably used for inactivating norovirus, which was difficult to inactivate with conventional virus removal agents.

[0043] In addition, the virus inactivator composition of the present invention has a very simple composition in which ethanol as a virus inactivating component and a specific organic acid and / or inorganic acid as a virus inactivation effect enhancing component are mixed with water to form an acidic aqueous solution. Therefore, it is easy to produce, and since it has a low ethanol concentration of 60 mass% or less and is acidic, it has little odor of ethanol and little irritation to skin, which are problems with many virus inactivators, and is extremely safe. In addition, it is easy to use because it does not have the problem of discoloration due to silver ions.

[0044] The present invention is not limited to the above-mentioned embodiment, and various modifications are possible within the scope of the claims, and embodiments obtained by appropriately combining the technical means disclosed in the different embodiments are also included in the technical scope of the present invention. The effects of the present invention will be explained in more detail below using examples, but the present invention is not limited to these examples. EXAMPLES

[0045] [Preparation of test solution] (A) Ethanol (manufactured by Wako Pure Chemical Industries, Ltd.), (B) fumaric acid, phosphoric acid, and citric acid monohydrate (all manufactured by Wako Pure Chemical Industries, Ltd.) were mixed in the mixing ratios (mass%) shown in Tables 1 and 3, and purified water was added to make 100 mass%, to obtain test solutions (present inventions 1 to 12).

[0046] (A) Ethanol (manufactured by Wako Pure Chemical Industries, Ltd.) (B) Fumaric acid, phosphoric acid, citric acid monohydrate, lactic acid, benzoic acid, trisodium phosphate, trisodium citrate dihydrate (all manufactured by Wako Pure Chemical Industries, Ltd.), and disodium fumarate (manufactured by Tokyo Chemical Industry Co., Ltd.) were mixed in the mixing ratios (mass %) shown in Tables 2 and 4, and purified water was added to make up 100 mass %, to obtain test solutions (Comparative Examples 1 to 16). EXAMPLES

[0047] [Test 1 for confirming virus inactivation effect (Feline calicivirus)] (Preparation of test virus solution) CRFK cells (JCRB cell bank) were cultured in a monolayer in a tissue culture dish using a cell growth medium made of MEM medium (Nacalai Tesque) supplemented with 10% fetal bovine serum. The cell growth medium was removed from the monolayer culture dish, and feline calicivirus (Feline calicivirus F-9 ATCC VR-782) was inoculated. Next, a cell maintenance medium made of MEM medium supplemented with 2% fetal bovine serum was added, and the cells were cultured for 1 to 5 days in a carbon dioxide incubator (CO2 concentration 5%) at 37 ± 1°C. Feline calicivirus is a type of non-enveloped virus, and is widely used as an alternative to norovirus, which cannot be cultured in cells.

[0048] After the incubation, the cell morphology was observed using an inverted phase contrast microscope to confirm that the cells had undergone morphological changes (cytopathic effect).The culture medium was then centrifuged at 1000 rpm for 3 minutes, and the resulting supernatant was ultrafiltered to obtain a test virus solution.

[0049] 0.1 mL of the test virus solution was added to 0.9 mL of the test solutions of the present invention 1 to 7 and comparative examples 1 to 11 prepared in Example 1 and mixed to prepare working solutions. After 1 minute, the working solutions were diluted 100-fold with MEM medium to prepare a 10-fold dilution series. The same procedure was carried out using purified water to which the test virus solution was added as a control.

[0050] (Measurement of viral infectivity) The cells used were cultured in a monolayer in a tissue culture microplate (96 wells) using cell growth medium, after which the cell growth medium was removed and 0.1 mL of cell maintenance medium was added to each well. Next, 0.1 mL of a 10-fold dilution series of the working solution was inoculated into each of four wells, and cultured for 4 to 7 days in a carbon dioxide incubator (CO2 concentration 5%) at 37 ± 1°C. After culture, the presence or absence of cell morphological changes (cytopathic effect) was observed using an inverted phase contrast microscope, and the 50% cell culture infectious dose (TCID 50 ) was calculated and converted into the infectivity titer per mL of the action solution, and compared with the infectivity titer of purified water used as a control, the log reduction in the infectivity titer was calculated.

[0051] The evaluation criteria for the virus removal effect were as follows: × if the log reduction in infectivity was 1 or less, △ if it was greater than 1 and less than 1.5, ○ if it was greater than 1.5 and less than 3, and ◎ if it was greater than 3. The evaluation results of the virus infectivity were calculated according to the composition of the test solution, pH, log 10 [(A) / (B)], along with the log reduction in infectivity, are shown in Tables 1 and 2.

[0052] [Table 1]

[0053] [Table 2]

[0054] As shown in Table 1, in Inventions 1 to 7, which contain (A) 10% by mass to 40% by mass of ethanol and (B) 0.05% by mass to 0.5% by mass of any of fumaric acid, phosphoric acid, and citric acid monohydrate, the log reduction in infectivity against feline calicivirus was greater than 1.5, confirming that the composition has a virus inactivation effect.

[0055] In particular, in Inventions 1 and 2 in which the blending amounts of ethanol and fumaric acid were 40% by mass and 0.2% by mass and 0.1% by mass, respectively, and Invention 5 in which the blending amount of ethanol was 40% by mass and the blending amount of phosphoric acid was 0.05% by mass, it was confirmed that the infectivity log reduction value was greater than 3, and a more significant virus inactivation effect was obtained. Furthermore, from the results of Inventions 1 to 7 and Comparative Examples 1 to 5, it was confirmed that when ethanol was blended alone, and when fumaric acid, phosphoric acid, or citric acid monohydrate was each blended alone, the infectivity log reduction value was less than 1, and no virus inactivation effect was observed, but that the virus inactivation effect was synergistically enhanced by blending ethanol with fumaric acid, phosphoric acid, or citric acid monohydrate.

[0056] In addition, from the results of Invention 6 in which the ethanol content was 10% by mass and the phosphoric acid content was 0.5% by mass, and Comparative Example 8 in which the ethanol content was 5% by mass and the phosphoric acid content was 0.5% by mass, it was confirmed that by making the ethanol content 10% by mass or more, the virus inactivation effect is synergistically enhanced by the addition of phosphoric acid.

[0057] Furthermore, from the results of Invention 5 in which the ethanol content was 40% by mass and the phosphoric acid content was 0.05% by mass, and Comparative Example 7 in which the ethanol content was 40% by mass and the phosphoric acid content was 0.02% by mass, it was confirmed that by setting the phosphoric acid content to 0.05% by mass or more, the virus inactivation efficacy of ethanol was enhanced.

[0058] Furthermore, in Comparative Examples 9 to 11, in which lactic acid, benzoic acid, and disodium fumarate were blended at 0.2 mass% instead of fumaric acid, phosphoric acid, and citric acid monohydrate, the log reduction in infectivity was 1.5, 0.7, and 1.33, respectively, and a sufficient virus inactivation effect was not observed. EXAMPLES

[0059] [Confirmation test for virus inactivation effect 2 (Feline calicivirus)] (Preparation of test virus solution) CRFK cells (JRBC cell bank) were cultured in a monolayer in a tissue culture dish using a cell growth medium made of MEM medium (Nacalai Tesque) with 10% fetal bovine serum. The cell growth medium was removed from the monolayer culture dish, and feline calicivirus (Feline calicivirus F-9 ATCC VR-782) was inoculated. Next, a cell maintenance medium made of MEM medium with 2% fetal bovine serum was added, and the cells were cultured in a carbon dioxide incubator (CO2 concentration 5%) at 37±1℃ for 1-5 days.

[0060] After the culture, the cell morphology was observed using an inverted phase contrast microscope to confirm that the cells had undergone morphological changes (cytopathic effect). The culture solution was then centrifuged at 1000 rpm for 3 minutes, and the resulting supernatant was used as the virus suspension. The virus suspension and a filter-sterilized 20% meat extract aqueous solution were mixed in a ratio of 3:1 to prepare the test virus solution. The test virus solution contained 5% meat extract as a load.

[0061] 0.1 mL of the test virus solution was added to 0.9 mL of the test solutions of the present invention 8 to 12 and comparative examples 12 to 16 prepared in Example 1 and mixed to prepare working solutions. After 1 minute, the working solutions were diluted 100-fold with MEM medium to prepare a 10-fold dilution series. The same procedure was carried out using purified water to which the test virus solution was added as a control.

[0062] The method for measuring the virus infectivity and the evaluation criteria were the same as in Example 2. The evaluation results of the virus infectivity were calculated according to the composition of the test solution, pH, log 10 [(A) / (B)], together with the log reduction in infection titer, are shown in Tables 3 and 4.

[0063] [Table 3]

[0064] [Table 4]

[0065] As shown in Table 3, in Inventions 8 to 12, which contain (A) 40% by mass to 60% by mass of ethanol and (B) 0.05% by mass to 0.2% by mass of any of fumaric acid, phosphoric acid, and citric acid monohydrate, the log reduction in infectivity against feline calicivirus was 2.5 or more, confirming that an immediate virus inactivation effect can be obtained even in the presence of a load from meat extract.

[0066] In contrast, as shown in Table 4, in Comparative Example 12, in which ethanol was blended alone at 60% by mass, and in Comparative Example 13, in which the blending amount of ethanol was 60% by mass and the blending amount of phosphoric acid was 0.02% by mass, the infectivity log reduction value was less than 1, and no virus inactivation effect was observed. In Comparative Examples 14 and 15, in which disodium fumarate and trisodium phosphate were blended instead of fumaric acid and phosphoric acid, the infectivity log reduction values ​​were 1.43 and 1.24, respectively, and no sufficient virus inactivation effect was observed. Furthermore, in Comparative Example 15, the pH of the test solution was 12.51, which was strongly alkaline, and there were concerns about problems such as corrosiveness and rough hands due to alkali.

[0067] In addition, in Comparative Example 16, in which trisodium citrate was used instead of citric acid monohydrate, the log reduction in infectivity was 1.93, indicating a virus inactivation effect. However, the pH of the test solution was alkaline at 9.15, raising concerns about corrosion and rough hands due to the alkali.

[0068] From the above results, it was confirmed that by mixing ethanol and one or more selected from fumaric acid, phosphoric acid, and citric acid monohydrate with water to prepare an acidic aqueous solution, a virus inactivation composition having sufficient virus inactivation effect against feline calicivirus and excellent safety can be obtained.

[0069] It was also confirmed that when combined with ethanol, fumaric acid, phosphoric acid, and citric acid monohydrate act as ingredients that enhance the virus inactivation efficacy of ethanol.

[0070] Although the inactivation effect of the virus inactivating composition of the present invention on enveloped viruses is not shown here, it goes without saying that the virus inactivating composition of the present invention also has a rapid inactivation effect on enveloped viruses, since enveloped viruses are more sensitive to drugs than non-enveloped viruses.

[0071] The present invention relates to a virus inactivator composition, a method for enhancing virus inactivation efficacy, and a virus inactivation method, which have a relatively low concentration of ethanol, a virus inactivating component, and which are acidic to weakly acidic and therefore highly safe, and which are particularly suitable for use as a virus inactivator composition that is directly applied by spraying or the like to places contaminated with viruses.

Claims

1. (A) 10% by mass to 60% by mass of ethanol as a virus inactivation component, (B) fumaric acid as a virus inactivation effect enhancing component, and (C) water are mixed to prepare an acidic aqueous solution; A virus inactivator composition, characterized in that the amount of the virus inactivation effect enhancing component is 0.05 mass% or more.

2. (A) 10% by mass to 60% by mass of ethanol as a virus inactivation component, and (B) one or more kinds selected from phosphoric acid and citric acid as a virus inactivation effect enhancing component are mixed with (C) water to prepare an acidic aqueous solution, A virus inactivator composition characterized in that the blending amount of the virus inactivation efficacy enhancing component is 0.05% by mass or more and 0.5% by mass or less (however, excluding the cases where the composition contains an extract of a plant of the genus Diospyros containing citric acid and tannin, where the fatty acid constituting the citric acid and the glycerin fatty acid ester is caprylic acid, where the composition contains a glycerin fatty acid ester and a grapefruit seed extract (obtained from grapefruit seeds and mainly composed of fatty acids and flavonoids), where the composition contains a phloroglucinol polymer which is a compound formed by polymerizing 7 or more molecules of phloroglucinol, where the composition contains lactic acid, citric acid, and a zinc-containing compound which liberates zinc ions in a solution, where the composition contains PEG / PPG-20-35 / 5-30 dimethicone, and where the composition contains an alcohol compound having 10 to 40 carbon atoms).

3. 3. The virus inactivating composition according to claim 1, comprising 30% by mass to 60% by mass of ethanol as the virus inactivating ingredient.

4. The virus inactivating composition according to claim 2 , wherein the virus is a non-enveloped virus.

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