Low-molecular weight water-soluble dietary fiber
By hydrogenating oligosaccharides, the challenge of inconsistent recognition as dietary fiber is addressed, resulting in a stable and accurate dietary fiber product with intestinal regulating effects.
Patent Information
- Application Number
- JP2023201629
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- Filing Date
- 2023-11-29
- Publication Date
- 2025-06-10
AI Technical Summary
Low-molecular-weight water-soluble dietary fibers, such as oligosaccharides, are not consistently recognized as dietary fiber due to variations in analysis methods, limiting their accurate and stable utilization.
Hydrogenating oligosaccharides to create a hydrogenated product that is recognized and quantified as dietary fiber, using methods like the AOAC 2001.03 enzyme-HPLC method.
The hydrogenated product of oligosaccharides can be used more accurately and stably as dietary fiber, providing a new low-molecular-weight water-soluble dietary fiber with intestinal regulating effects.
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Abstract
Description
Technical Field
[0001] The present invention relates to low-molecular-weight water-soluble dietary fiber.
Background Art
[0002] When food is ingested, indigestible polysaccharides and lignins that are not hydrolyzed by human digestive enzymes are regarded as dietary fiber, and are roughly classified into water-soluble dietary fiber and insoluble dietary fiber. Insoluble dietary fiber such as cellulose and hemicellulose, and high-molecular-weight water-soluble dietary fiber such as pectin and sodium alginate that form a precipitate in about 80% ethanol are well known to have an intestinal regulating effect. In recent years, as the importance of low-molecular-weight water-soluble dietary fiber defined as dietary fiber that is soluble even in about 80% ethanol has been elucidated, these have also come to be noticed.
[0003] Thus, although dietary fiber seems to be established as a category, there are some substances that are not recognized as dietary fiber depending on the analysis method. For example, oligosaccharides defined as carbohydrates in which 2 to 10 monosaccharides are glycosidically bonded are classified as low-molecular-weight water-soluble dietary fiber, and the intestinal regulating effect has also been mentioned (Patent Document 1), but there are cases where they are not recognized as dietary fiber depending on the analysis method.
Prior Art Documents
Patent Documents
[0004]
Patent Document 1
Summary of the Invention
Problems to be Solved by the Invention
[0005] An object of the present invention is to provide a new low-molecular-weight water-soluble dietary fiber.
Means for Solving the Problems
[0006] Although oligosaccharides may not be recognized as dietary fiber depending on the analytical method, the inventors of the present application have found that by hydrogenating oligosaccharides, they can be recognized as dietary fiber, and thus completed the present invention. That is, the present invention relates to the following (1) or (2). (1) Use of a hydrogenated product of an oligosaccharide as dietary fiber. (2) The use according to (1) above, wherein the oligosaccharide is an oligosaccharide in which 3 to 10 monosaccharides are polymerized.
Effect of the Invention
[0007] Although oligosaccharides may not be recognized as dietary fiber depending on the analytical method, it has not been conventionally known that they can be recognized as dietary fiber by hydrogenation. Therefore, since it can be expected that the hydrogenated product of oligosaccharides can be used more accurately and stably as dietary fiber compared to non-hydrogenated oligosaccharides, the present invention can provide a new low-molecular-weight water-soluble dietary fiber.
Mode for Carrying Out the Invention
[0008] The oligosaccharide used in the present invention may be any oligosaccharide having 2 or more polymerized sugars, but oligosaccharides having a degree of polymerization of 3 or more are preferably used. There is no particular upper limit to the number of polymerized sugars, but it is usually 10 or less, preferably 6 or less, and more preferably 5 or less. The number of polymerized sugars can be determined using liquid chromatography capable of separating sugars by a conventional method. As the hydrogenated product of an oligosaccharide, any sugar having a structure in which the formyl group or carbonyl group of the sugar constituting the oligosaccharide is hydrogenated can be used.
[0009] As a method for hydrogenating an oligosaccharide, a conventional method can be used, or a commercially available hydrogenated product of a sugar may be used. The hydrogenated product of a sugar is not limited to one type, and a mixture of a plurality of types may be used, or a reduced starch saccharide known as reduced maltose may be used. The hydrogenated sugar in the present invention can be used in the same manner as other dietary fibers in foods and drinks, feeds, etc., where dietary fiber can be used. In the present invention, dietary fiber is defined as "the total of indigestible components in food that are not digested by human digestive enzymes", and based on this definition, the quantification of dietary fiber in the present invention is carried out by the AOAC official method defined by AOAC International. However, since low-molecular-weight water-soluble dietary fiber cannot be quantified by the enzyme-weight method (Prosky method) or the modified Prosky method, the enzyme-HPLC method (AOAC 2001.03 method or AOAC 2011.25 method) is used. Examples of the present invention are shown below, but the present invention is not limited to these examples.
Example
[0010] Hereinafter, as a reduced starch saccharide containing oligosaccharide, commercially available reduced starch saccharides (hydrogenated products) (A to H) and raw materials (before hydrogenation) of the hydrogenated products (C to H) were subjected to the AOAC 2001.03 method (enzyme-HPLC method), and the results of quantification as the amount of dietary fiber (g) per 100 g of solid content are shown in Table 1 as "dietary fiber amount" and "raw material dietary fiber amount", respectively. The enzymes used were Termamyl 120L (manufactured by Novo Nordisk), Protease E-BSPRPD (manufactured by Megazyme), and Amyloglucosidase A-9913 (manufactured by Sigma-Aldrich) according to the conventional method. Table 1 shows the contents (mass%) of disaccharides, trisaccharides, tetrasaccharides, and sugars having a degree of polymerization of 5 or more in each reduced starch saccharide.
[0011]
Table 1
[0012] As shown in Table 1, reduced starch saccharides containing a large amount of hydrogenated oligosaccharides have a larger amount quantified as dietary fiber compared to starch saccharides containing non-hydrogenated oligosaccharides. Considering that the definition of dietary fiber is "the total of indigestible components in food that are not digested by human digestive enzymes", it can be expected to exhibit the effects as dietary fiber.
Claims
1. Use of a hydrogenated oligosaccharide as a dietary fiber.
2. The use according to claim 1, wherein the oligosaccharide is an oligosaccharide in which 3 to 10 monosaccharides are polymerized.
Citation Information
Patent Citations
Water-soluble dietary fiber-containing composition and method for preparing same
JP2006254901A