Oral cosmetic

Abstract

To provide an oral cosmetic which prevents dental caries, gingivitis, periodontitis or the like by maintaining a good balance of bacterial flora in the mouth and is stable over a long period of storage.SOLUTION: There is provided an oral cosmetic which contains, as prebiotics, a herb oil extracted from herbs, humic shale or plant minerals extracted from humic shale, EM-X which is an active fermentation ingredient, a plant-derived antibacterial or bactericidal agent and an antioxidant. In addition, the oral cosmetic further contains an interbacterial nutrient network control component and a Th17 cell activation prevention component. The herbal oil is extracted by a low-temperature extractive distillation method. The biochemical effects are diverse and suggest a contribution to the maintenance and improvement of oral health. The biochemical effects are diverse and suggests the contribution to the maintenance and improvement of oral health. Thus, a product containing herbal raw materials is likely to be useful in oral care and can be combined with conventional care methods to achieve more effective care.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to oral cosmetics.

Background Art

[0002] Oral cosmetics are required to prevent dental caries, gingivitis, periodontitis, etc. by maintaining a good balance of the oral bacterial flora, and to have an effect on skin care of the skin by the balance of the skin resident flora. Oral resident bacteria and skin resident bacteria exist in different environments, but are related in many aspects such as the host immune response, barrier function, immune regulation, biochemical interaction, symbiotic relationship, etc. Conventionally, attention has been paid to bactericidal and antibacterial effects, and there are extremely few oral care agents or oral cosmetics for maintaining the balance of the bacterial flora to prevent dental caries, gingivitis, periodontitis, etc. and performing skin care of the skin by the balance of the skin resident flora.

[0003] Non-Patent Document 1 reveals that Fusobacterium, an oral resident bacterium, produces polyamines through interaction with other resident bacteria via amino acids, and this polyamine enhances the pathogenicity of the biofilm of the periodontal pathogen Porphyromonas gingivalis, indicating that it is possible to prevent periodontal disease by controlling the nutritional network between bacteria. A biofilm is an aggregate of various microorganisms formed on a solid phase surface, and dental plaque is one of the biofilms, which causes dental caries and periodontal disease.

[0004] Non-Patent Document 2 states that helper T cells that respond to periodontal pathogenic bacteria as a cause of periodontal disease flow from the mouth to the intestine, are activated under the influence of intestinal bacteria, and then move back to the mouth to cause the onset and exacerbation of periodontal disease. Therefore, prevention of periodontal disease is expected by drugs targeting intestinal bacteria.

[0005] Patent Document 1 discloses medicinal ingredients having antibacterial or bacteriostatic effects. As medicinal ingredients, organic acids such as lactic acid, citric acid, gluconic acid, succinic acid, fumaric acid, and malic acid, polyphenols such as flavonoids, catechins, tannins, carnosol, curcumin, and coumarin, monoterpenes such as perillaldehyde, pinene, and hinokitiol, sesquiterpenes such as zingiberene, terpenes such as diterpenes, isothiocyanates such as allyl isothiocyanate, allyl sulfides such as allicin, saponins such as ronicerin, etc. are disclosed. Furthermore, capsaicin, menthol, piperine, cinnamaldehyde, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, calcium sorbate, propionic acid, sodium propionate, calcium propionate, calcium hypochlorite, paraoxybenzoic acid ester, sucrose fatty acid ester, sodium dehydroacetate, sodium acetate, glycerin fatty acid ester, chitosan, pectin degradation product, glycine, ε-polylysine, alginic acid, xylitol, casein, lactoferrin, protamine, lysozyme, niacin, etc. are disclosed.

[0006] Also, natural-derived medicinal ingredients are disclosed, such as persimmon extract, laurel extract, mastic extract, tea extract, wasabi extract, mustard extract, ginger extract, pepper extract, myoga extract, capsicum extract, citrus extract, grapefruit seed extract, kiwi extract, apple extract, big leaf extract, egonoqi extract, kawarayomogi extract, hoonoki extract, rengyou extract, ivy extract, garlic extract, leek extract, turmeric extract, cumin extract, clove extract, coriander extract, rosemary extract, cinnamon extract, mosou bamboo extract, kuma sasakura extract, mushroom extract, lotus root extract, hop extract, pine extract, Korean ginseng extract, neem extract, bay oil, oregano oil, anise oil, thyme oil, lemongrass oil, garlic oil, wikyou oil, cinnamon oil, peppermint oil, spearmint oil, eucalyptus oil, propolis, lactic acid bacteria, etc.

[0007] As an oral cosmetic or a component of an oral cosmetic, for example, hinokitiol is known to have a bactericidal action and a tissue astringent action, and is used for the purpose of subsiding symptoms in stomatitis or gingivitis, treating periodontal disease, candidiasis, etc. However, since hinokitiol is a substance that is inherently sublimable and is further decomposed by light, when formulated as an oral composition, there are problems such as a decrease in antibacterial activity, poor storage stability, discoloration and phase separation over time when stored for a long time.

[0008] Patent Document 2 discloses, with respect to the above problems of hinokitiol, formulating one or more edetates selected from disodium edetate, trisodium edetate, dipotassium edetate dihydrate, and tetrasodium edetate dihydrate, ethanol, and water in an appropriate ratio in an oral composition containing hinokitiol. Thereby, the stability of hinokitiol in the preparation is enhanced, a high antibacterial activity is exhibited, hardly any discoloration occurs even after long-term storage, the phase separation stability is excellent, and a preparation excellent in storage stability over time is obtained.

[0009] and Patent Document 3 discloses that, based on the finding that the bactericidal action of hinokitiol is relatively low and the number of pathogenic bacteria in the oral cavity cannot be sufficiently reduced, (a) hinokitiol, (b) at least one fluorine compound selected from the group consisting of fluorides of alkali metals, fluorophosphoric acid, and salts of fluorophosphoric acid, and (c) menthol are contained, and further, (a) per 1 part by weight of hinokitiol, (b) 1.25 parts by weight or more of the fluorine compound, and (c) 7.5 parts by weight or more of menthol are contained, whereby the bactericidal action of hinokitiol is dramatically improved.

[0010] Patent Document 4 discloses an oral pathogenic bacteria bactericide composed of persimmon tannin having a purity of 30% or more obtained from persimmon astringent extract and oligosaccharides of non-fermentable tetrasaccharides or less. Persimmon tannin, which has a high binding force with protein components, adsorbs pathogenic bacteria and reacts to remove (destroy) them.

[0011] ​Patent Document 5 discloses methods and compositions for treating infections and modulating the microbiota of the skin and mucous membranes to treat diseases or disorders associated with or exacerbated by dysbiosis. Methods and compositions are provided that include hogosidin peptides (SH-lantibiotics), derivatives, and variants. Also provided are compositions including methods and probiotic compositions that utilize strains of Staphylococcus hominis and Staphylococcus epidermidis that produce hogosidin, hogosidin-like peptides, or other inhibitors of skin pathogens.

[0012] Patent Document 6 discloses a cosmetic agent composed of galactomannan having a molar mass of at least 5 to 630 kDa, preferably 5 to 120 kDa, more preferably 8 to 80 kDa, and crosslinked sulfated galactan having a molar mass of 7 to 3000 kDa, preferably 7 to 1100 kDa, more preferably 8 to 200 kDa, in a physiologically acceptable medium, and b) a hydrolyzate of Theobroma cacao L beans. Also disclosed is a cosmetic composition containing the hydrolyzate of the cosmetic agent composed of galactomannan and crosslinked sulfated galactan and the Theobroma cacao L beans, which in particular maintains the balance of the skin microbiota and promotes and / or improves makeup retention.

Prior Art Documents

Patent Documents

[0013]

Patent Document 1

Patent Document 2

Patent Document 3

Patent Document 4

Patent Document 5

Patent Document 6

Non-Patent Document 1

Non-Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0014] Conventionally, various types of toothpastes with bactericidal and antibacterial effects in the oral cavity have been proposed. However, the main purposes have been to prevent dental caries, gingivitis, and periodontitis by further improving the bactericidal and antibacterial effects and to achieve stability against long-term storage.

[0015] In recent years, research on the human microbiome due to the progress of molecular biology has revealed that the human body harbors pathogens, which are microorganisms that cause diseases. However, pathogens do not cause diseases in healthy people and simply coexist with the microbiome, which is an aggregate of microorganisms that live in the host or human body. It has been found that when the balance of this aggregate is disrupted and fluctuations occur in the aggregated bacteria, the pathogens can harm the human body. That is, the mechanism by which the physiological functions of commensal bacteria are exerted is different from the mechanism by which a single type of pathogenic bacterium causes an infectious disease. From the conventional killing war against bacteria regarded as villains, future medicine is required to maintain the balance of commensal bacteria that live in the living body based on symbiosis.

[0016] Essential oils of higher plants have excellent disinfection, antibacterial, wound healing, and anti-inflammatory effects. They are used as pharmaceuticals in Europe and the United States and are registered under the new international standardization agency regulations. By clinically applying essential oils of plants, an expected consensus effect on the oral flora that causes oral infections can be achieved. As its mechanism of action, the inhibition of biofilms (dental plaque) by essential oils of plants is considered to include the influence of essential oils on the quorum sensing signals of bacteria within the biofilm, the destruction of the extracellular matrix of the biofilm, and good permeability into the biofilm.

[0017] The same interpretation is considered applicable to Porphyromonas gingivalis (Pg) bacteria, which are the Keystone of the main causative bacteria of periodontal disease in the oral flora, and other periodontal disease-causing bacteria. It has a therapeutic effect not only on periodontal disease, which is a chronic disease of the oral cavity, but also on candidiasis and stomatitis.

[0018] On the other hand, regarding the skin, various microorganisms exist on its surface, and these play an important role in maintaining the balance of the skin. These microorganisms include bacteria, fungi, viruses, etc., and are usually called the "skin microbiota" or "skin flora". The skin microbiota contributes to various health functions such as the regulation of the immune system on the skin surface and the prevention of pathogen invasion.

[0019] The microbiota of the skin and the oral cavity are closely related to the immune response. A healthy microbiota is considered to regulate the host's immune response and enhance the defense against pathogens. The issue is how to balance the bacterial flora and improve the oral environment.

[0020] In response to these issues, based on the latest research results, the present invention aims to provide an oral cosmetic that can prevent dental caries, gingivitis, periodontitis, etc. by maintaining a good balance of the oral bacterial flora and is effective for skin care of the skin due to the balance of the skin resident flora.

Means for Solving the Problems

[0021] (1) An oral cosmetic, the components of which are composed of a prebiotic agent of natural materials that promotes the growth of beneficial bacteria, vegetable minerals, vitamin agents, and EM (Effective Microorganisms) agents, and which is characterized by maintaining the balance of the oral resident flora and the balance of the skin resident flora in weakly acidic skin due to the synergistic effect of the prebiotic agent, the vegetable minerals, the vitamin agents, and the EM agents.

[0022] (2) Further characterized by containing fermented garlic.

[0023] (3) Further characterized by containing an inter-bacterial nutrient network control component.

[0024] (4) Further characterized by containing a Th17 cell activation prevention component.

[0025] (5) Further characterized by containing an antibacterial and preservative agent derived from plants.

[0026] (6) The prebiotic agent of the natural materials is preferably a herb.

[0027] (7) The herb is preferably chamomile, lavender, lemongrass, sage herb, or rosemary.

[0028] (8) It is preferable that the chamomile uses the flowers of the chamomile extracted as chamomile oil.

[0029] (9) It is preferable that the lavender uses the flowers of the lavender extracted as lavender oil.

[0030] (10) It is preferable that the lavender oil is extracted only from the flower buds of true lavender by natural cultivation.

[0031] (11) Preferably, the lemongrass is extracted and used as lemongrass essential oil from at least one of the leaves and stems of the lemongrass.

[0032] (12) Preferably, the sage herb is extracted and used as sage herb essential oil from at least one of the leaves and flowers of the sage herb.

[0033] (13) Preferably, the rosemary is extracted and used as rosemary essential oil from at least one of the leaves and flowers of the rosemary.

[0034] (14) Preferably, the chamomile oil, the lavender oil, the lemongrass essential oil, the sage herb essential oil, and the rosemary essential oil are produced and extracted by a low-temperature distillation extraction method that does not destroy the components.

[0035] (15) Preferably, the plant-derived mineral is fulvic acid collected from humic shale.

[0036] (16) Preferably, the plant-derived antibacterial and preservative is phytic acid, hinokitiol, aged garlic extract, or mugwort extract.

[0037] (17) Preferably, the vitamin agent is vitamin C or vitamin E.

[0038] (18) Preferably, the bacterial inter-nutrient network control component is lavender floral water extracted only from the flower buds of organically grown lavender.

[0039] (19) Preferably, the lavender floral water is extracted by a low-temperature distillation extraction method.

[0040] (20) The Th17 cell activation prevention component is preferably persimmon tannin.

[0041] (21) An oral cosmetic, comprising purified water as water, thick glycerin, propylene glycol, and butylene glycol as humectants, sodium polyacrylate as a binder, 1-menthol as a cooling agent, peppermint oil as a flavoring agent, herbal oil as a probiotic, fulvic acid collected from humic shale, EM bacteria or fermented garlic as a fermentation active ingredient, phytic acid, hinokitiol, lemongrass, aged garlic extract or mugwort extract as an antibacterial and bactericidal agent derived from plants, vitamin E or vitamin C as an antioxidant, lavender floral water extracted only from the flower buds of true lavender grown by natural cultivation as a bacterial inter-nutritional network control component, lavender floral water extracted only from the flower buds of true lavender grown by natural cultivation as a bacterial inter-nutritional network control component, and persimmon tannin as a Th17 cell activation prevention component.

Advantages of the Invention

[0042] The oral cosmetic of the present invention can maintain a good balance of the oral flora, thereby preventing dental caries, gingivitis, periodontitis, etc., and has an effect on skin care by the balance of the skin resident flora.

Brief Description of the Drawings

[0043]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Mode for Carrying Out the Invention

[0044] The present invention relates to an oral care cosmetic, which is composed of a prebiotic agent of a natural material that promotes the growth of beneficial bacteria, a plant mineral, a vitamin agent, and an EM (Effective Microorganisms) agent. Due to the synergistic effect of the prebiotic agent, the plant mineral, the vitamin agent, and the EM agent, it is characterized by maintaining the balance of the oral resident flora and the balance of the skin resident flora in weakly acidic skin. It may further contain fermented garlic.

[0045] Even if these components (constituent elements) are already known components, it is not simply a matter of combining them. It is a common experience that even if the names are the same, the effects vary depending on which material is used and how it is manufactured. The components were determined by considering materials carefully selected by the inventor and further considering the manufacturing method, and the oral care cosmetic was obtained as a result of verifying the synergistic effect of the combination of the components through repeated clinical trials. Therefore, it is not a simple combination, but by clarifying the purpose and conducting clinical trials by combining various components to see whether a synergistic effect can be obtained and the balance of the oral resident flora can be achieved, the present invention cannot be reached.

[0046] The development objective of the oral cosmetics according to the present invention is to address the global issues of side effects associated with the spread of antibiotics and the increase in drug-resistant bacteria. According to research in the Periodontology Department of the hospital affiliated with Nihon University School of Dentistry, among the streptococci in the oral cavity of periodontal disease patients who received long-term local antibiotic therapy, 14.6% showed tetracycline resistance, and various resistant bacteria have been detected in many subjects. Furthermore, with the progress of molecular biology, it has been found that humans carry pathogens that may cause diseases. These pathogens do not cause problems in healthy humans and simply coexist with the aggregate of microorganisms present in the human body. However, when the balance of this aggregate is disrupted, it has been confirmed that the pathogens can harm humans. This is different from the conventional understanding that a single pathogen causes an infectious disease. Modern medicine is required to maintain the balance of symbiosis with the resident bacteria present in the body, rather than simply regarding bacteria as enemies.

[0047] This product embodies that finding and proposes a new approach to maintaining health by promoting symbiosis with resident bacteria. This innovative oral cosmetic aims beyond mere prevention to achieve true health through symbiosis with resident bacteria. The developers took 10 years from soil improvement to securing herb materials through organic cultivation, product development, and clinical verification of patients. As a result, the treatment method based on natural materials can reduce the use of antibiotics and is believed to play an important role in modern medicine as a valuable approach to stopping the spread of drug-resistant bacteria. It is convinced that continuous research and development will open up new prospects in this field.

[0048] The problem of drug-resistant bacteria to antibiotics has become one of the important issues in modern medicine. With the increase in drug-resistant bacteria, it is urgent to save antibiotics as much as possible and develop new treatment methods based on natural materials. Focusing on this issue, the development of treatment methods using natural materials is urgently needed. It is known that natural materials have antimicrobial and anti-inflammatory properties, and the development of treatment methods that utilize these properties to reduce the use of antibiotics is being aimed at.

[0049] The selection of natural materials is an important step, and plant-derived herb materials, components derived from fermented foods, microbial extracts, etc. are regarded as promising. These materials have the potential to have a wide range of effects on cell processes. Using component analysis, molecular biology, and biochemical approaches of natural materials selected from many literatures, the active ingredients and their physiological activities of each material were verified in detail, and components with antimicrobial activity and immunomodulatory effects were identified in particular at this stage. Studies on the specific effects of the antibacterial effects and immunomodulatory abilities of each natural material on cell processes have been reported. This includes in vitro and in vivo experiments, which have clarified how natural materials affect the immune response and inflammation regulation of cells.

[0050] Research on treatment methods based on natural materials will continue in the future, and the discovery of new materials and combinations is expected. It will be possible to develop new treatment methods for the treatment of infectious diseases and other diseases, and progress in the medical field is expected.

[0051] Treatment methods based on natural materials usually have few or almost no side effects. Since the risk of common side effects and the generation of drug-resistant bacteria is low like antibiotics, it is a safe option for patients. The large-scale use of antibiotics is also a concern for the environment, and treatment methods using natural materials are highly likely to reduce the environmental burden, so they are also attracting attention as sustainable medical solutions.

[0052] Regarding the expectation of the antibacterial effect by multiple herb materials as an alternative to antibiotics, from a molecular and biochemical perspective, combining multiple herb materials may enable different antibacterial components to cooperate with each other and exert a more powerful antibacterial effect against a wide range of bacterial species. Different herbs contain different antibacterial substances, and the cooperation of these components increases the possibility of combating resistant bacteria. Herb materials have antibacterial effects such as changing the permeability of cell membranes and inhibiting intracellular metabolic processes, thereby preventing the growth and proliferation of microorganisms and contributing to the treatment and prevention of infectious diseases. Frequent use of a single antibiotic increases the risk of microorganisms acquiring resistance to that antibiotic. A combination of antibacterial substances extracted from multiple herb materials may prevent the occurrence of resistance. It is considered that it is difficult for microorganisms to combat multiple antibacterial components simultaneously, making it difficult for them to acquire resistance.

[0053] Many herb materials have other health effects such as antioxidant, anti-inflammatory, and immunomodulatory effects in addition to the antibacterial effect. These health effects may help strengthen the immune system and enhance the body's resistance when combating infectious diseases. General antibiotics may cause side effects and toxicity when used at high concentrations, while many herb materials are effective even at low concentrations and have a low risk of side effects, so they are considered to be highly safe. For the above reasons, antibacterial therapy combining multiple herb materials is being studied as a promising alternative to antibiotics.

[0054] Herbal materials have a great impact on intracellular processes through their natural compounds and contribute to human health. For example, polyphenols, carotenoids, flavonoids, etc. affect intracellular processes. These components exhibit antioxidant effects and support cell health by reducing oxidative stress in cells. Specific herbs promote the activation of immune cells, improving resistance to infections and inflammation. They also regulate energy production, glucose metabolism, lipid metabolism, etc. Many herbal materials have anti-inflammatory effects, calming cell inflammation and assisting tissue repair. This is useful for the treatment of chronic inflammatory diseases such as periodontal disease. Thus, due to their diverse biochemical properties, herbal materials have a great impact on human cell processes and are useful for maintaining health, preventing diseases, and treating illnesses.

[0055] Consider the pharmacological effects on cell processes regarding the reasons for selecting the components of rosemary, lemongrass, lavender, sage herb, chamomile, and tea tree from many herbs.

[0056] The antioxidant components of rosemary reduce oxidative stress in cells, decrease oxidative damage to cells, maintain cell health, and contribute to delaying aging.

[0057] The anti-inflammatory components of lemongrass suppress the production of inflammatory cytokines, have the effect of calming inflammation, and are useful for the management of inflammatory diseases.

[0058] The relaxation effect of lavender is that the fragrance components act on the nervous system, induce relaxation, and contribute to reducing stress hormones and improving sleep quality.

[0059] Sage herb has an antibacterial effect that suppresses the growth of bacteria in the oral cavity, maintains oral health, and also has a protective effect on oral cells. The sedative components of chamomile act on nerve cells and contribute to relieving anxiety and tension.

[0060] The antibacterial components of tea tree have antibacterial effects against bacteria and fungi, protect skin cells, and are useful for preventing infections.

[0061] These pharmacological effects are due to the specific biochemical components of the herbal materials affecting receptors and enzymes within cells. Through appropriate combinations and usage methods of the herbal materials, these pharmacological effects can be maximized and utilized for health promotion, disease prevention, and treatment.

[0062] Consider the reasons for adding the fermented components of garlic.

[0063] (1) It has a strong antibacterial effect, and especially fermented garlic is rich in antibacterial compounds such as allicin. By adding this to the herb blend, the growth of microorganisms can be controlled more effectively, strengthening the defense against harmful bacteria and fungi in the oral cavity and helping to maintain oral health.

[0064] (2) It also affects the immune system and supports the activation of immune cells. This improves resistance to infections and enhances the immune response in the body. The activation of the immune system in the oral cavity contributes to the defense against oral infections and inflammation.

[0065] (3) It helps reduce bad breath. Garlic contains sulfur compounds, which have the effect of suppressing the microorganisms that cause bad breath. Blending with herbs can be expected to reduce bad breath. It improves the balance of microorganisms in the oral cavity and contributes to suppressing the occurrence of bad breath.

[0066] (4) Garlic also has an anti-inflammatory effect and helps relieve oral inflammation. Oral inflammation can cause gingivitis and stomatitis, but the anti-inflammatory components of garlic contribute to the alleviation of these symptoms.

[0067] (5) The fermented components of garlic regulate the balance of microorganisms in the oral cavity and promote the growth of beneficial bacteria. This improves the oral ecosystem and supports oral health. Although it can cause gingivitis and stomatitis, the anti-inflammatory components of garlic contribute to the alleviation of these symptoms.

[0068] Overall, the combination of herb materials and fermented garlic is considered to bring many benefits to the oral environment, such as adjusting the microbial balance in the oral cavity, maintaining oral health, and enhancing the immune response. This increases the likelihood of contributing to the promotion of oral health.

[0069] Consider the effects of adding plant-based minerals.

[0070] (1) Plant minerals play a role in supporting the absorption of nutrients in the oral cavity. Minerals such as calcium and magnesium are particularly important for dental and bone health, and the effective absorption of these minerals contributes to oral health. By ingesting them together with the components of herb materials and fermented garlic, the absorption of these nutrients increases.

[0071] (2) They have an anti-inflammatory effect and help reduce inflammation in the oral cavity. By reducing oral inflammation, oral cells function more normally, permeability improves, and thus the defense against infection and inflammation is strengthened.

[0072] (3) Also, plant-based minerals are known as elements necessary for tissue repair. When oral tissues are damaged, plant-based minerals promote the tissue regeneration process, support repair, and effective repair of oral tissues can improve permeability.

[0073] (4) They have an antioxidant effect and protect oral cells from oxidative stress. Oxidative stress can damage oral cells and increase permeability, and the antioxidant effect of plant-based minerals helps maintain oral cells in a healthier state. The addition of plant minerals contributes to improved permeability in oral cell processes, helps maintain and repair oral health. This improves the oral environment and promotes oral health.

[0074] EM-X (Registered Trademark: Efficient Microorganisms-X Gold) is a fermented liquid composed of beneficial microorganisms. Consider the effects of its formulation.

[0075] (1) The fermentation broth of the useful microorganisms contained in EM-X helps to regulate the oral microbiota. By restoring the balance in the oral cavity, the growth of harmful bacteria is suppressed, contributing to the prevention of bad breath and periodontal diseases.

[0076] (2) It neutralizes the acidic state and adjusts the pH balance in the oral cavity. This reduces the vulnerability of tooth enamel and supports dental health.

[0077] (3) EM-X also contains microorganisms that produce antioxidants, which help protect cells from oxidative stress in the oral cavity. This alleviates stomatitis and oral inflammation and supports the normal progression of cell processes.

[0078] (4) EM-X is expected to have an effect of suppressing inflammation in the oral cavity, improving gum health, and reducing the impact of inflammation on cell processes.

[0079] (5) Since EM-X contains materials that regulate the immune response, it improves the immune function in the oral cavity. This enhances resistance to infections and regulates the immune response in cell processes.

[0080] (6) The materials contained in EM-X affect the biofilms formed by oral bacteria. Biofilms are aggregates of bacteria and are the main components of dental plaque and tartar. By suppressing the formation and accumulation of biofilms, EM-X helps to maintain dental cleanliness.

[0081] (7) Periodontal pockets are the grooves formed between the gums and teeth and are associated with the onset of periodontal diseases. If EM-X can regulate the microbiota in periodontal pockets and reduce inflammation, it will contribute to the prevention of periodontal diseases.

[0082] Considering the safety to the human body, optimal conditions are required from soil improvement in the cultivation of herbal materials to harvesting and extraction methods.

[0083] (1) The importance of organic cultivation is that organic farming restricts the use of synthetic pesticides and chemical fertilizers, reducing the accumulation of harmful chemicals in the soil and plants. As a result, herbs grow in a natural state, minimizing harmful residues.

[0084] (2) The effect of EM fermentation broth is to contribute to the decomposition of organic matter and the activation of microorganisms. This improves the health of the soil and creates an environment suitable for the growth of herbs. Also, the EM fermentation broth helps control harmful soil microorganisms and increase beneficial microorganisms.

[0085] (3) The timing of harvesting needs to be appropriately carried out according to the growth cycle and the concentration of components. If harvesting is too early, there may be a shortage of active ingredients, and if it is too late, the quality will decline. It is important to determine the harvesting timing based on scientific guidelines.

[0086] (4) The selection of the extraction method is suitable for methods with low temperature, such as the low-temperature extraction method, which does not deteriorate the useful components. Also, it is essential to establish a high-quality extraction method that includes selecting an appropriate solvent and maintaining the quality.

[0087] (5) Inspection and quality control require quality control at each stage of cultivation, harvesting, and extraction. Through component analysis and microbial testing of herb materials, safety and quality are confirmed. A strict inspection process has been introduced to provide consistent high-quality herbs.

[0088] (6) Efforts towards sustainability may affect the ecosystem, and sustainable farming methods, efficient use of water resources, and consideration for the ecosystem are required. It is necessary to adopt an approach based on the SDGs (Sustainable Development Goals) and carry out production while minimizing the environmental impact.

[0089] The human body has resident bacteria of the human microbiome (microorganisms) that are specific to the intestine, oral cavity, nasal cavity, skin, vagina, etc., and coexist with human organs. Oral resident bacteria form the ecosystem in the oral cavity and play various roles such as assisting digestion, regulating immune responses, maintaining dental health, preventing bad breath, maintaining the health of host cells, and performing barrier functions. Research on the interaction between microorganisms and host cells has the potential to lead to the development of new therapies and health foods.

[0090] By understanding the mechanisms of diseases involving microorganisms and finding ways to regulate the balance of the microbiota, the development of new therapies and products becomes possible. For the development of microbiota-based therapies and health products, biotechnology methods such as gene editing, probiotics (the action of beneficial bacteria themselves), and prebiotics (the action of plant components that promote the growth of beneficial bacteria) may be applied.

[0091] Recent research has verified that changes in the composition of the human microbiome can affect health and disease. Research on the interaction between microorganisms and host cells leads to the development of new therapies and preventive methods. It is expected that the conventional classification and treatment methods of diseases will change to a new approach that takes into account the influence of microorganisms. If changes in the microbiota are associated with a specific disease, monitoring such changes and predicting the risk of the disease will enable preventive measures to be taken. Systemic diseases induced by oral infections can be understood from the relationship between resident bacteria and the human body.

[0092] The background is as follows: (1) Oral infections such as dental caries and periodontal diseases are infections caused by the disruption of the balance (dysbiosis) of the resident bacterial flora in the oral cavity; (2) Immune cells, which are the main players in the body's defense function, circulate throughout the body including oral tissues via the blood; (3) The oral cavity is part of the digestive organs, and all foods important for maintaining health and immune function are ingested through the oral cavity.

[0093] Numerous studies have proven that, both bacteriologically and immunologically, "oral information is transmitted throughout the body, and systemic information is transmitted to the oral cavity." The herb-based prebiotics developed based on scientific evidence not only regulate the balance of the oral microbiota but also have a beneficial effect on the health of the oral mucosa. The oral microbiota is closely related to the oral mucosa, and these interactions play an important role in oral health.

[0094] Herbs contain bioactive components such as antibacterial and anti-inflammatory polyphenols, flavonoids, and terpenoids. These components have the effect of suppressing harmful bacteria and inflammation in the oral cavity and contribute to the improvement of the oral environment. In particular, the oral mucosa is highly sensitive to external stimuli and environmental changes and is a tissue prone to inflammation and damage. Therefore, the anti-inflammatory effect and protective action of herbs have a good impact throughout the body.

[0095] Persimmon tannin is an antioxidant that counteracts oxidative stress substances called free radicals generated inside cells or in the body. Free radicals can cause oxidative damage and inflammation inside cells, and persimmon tannin has the effect of suppressing these harmful reactions. The antioxidant effect helps prevent damage to DNA and cell membranes and contributes to maintaining cell health.

[0096] In addition, it suppresses the production of inflammatory cytokines and alleviates the inflammatory response. The inflammatory response inside cells can cause diseases and tissue damage, but persimmon tannin controls this. It also exhibits antibacterial action and suppresses the growth of bacteria. To suppress the growth of bacteria in the oral cavity, it is effectively used in oral care. Furthermore, persimmon tannin is said to have an antiviral action against some viruses and may suppress virus infection. Through these mechanisms of action, persimmon tannin contributes to reducing oxidative stress and inflammation inside cells and improving resistance to bacteria and viruses. However, further research is needed on the specific molecular-level mechanism of action of persimmon tannin.

[0097] There are 700 species (identified) of bacteria in the oral cavity that exist as oral commensal bacteria (oral flora), and the relationship between the disturbance of the bacterial flora (dysbiosis) and various oral diseases has been elucidated. Conventional chemotherapy eliminates not only pathogenic bacteria but also commensal bacteria, resulting in the disruption of the balance of the oral commensal flora. Natural herb materials contain many phytochemicals as prebiotics for oral commensal bacteria, and these components have antibacterial, anti-inflammatory, antioxidant effects, etc. The aromatic molecules that make up plant essential oils regard pathogenic bacteria as invaders that disrupt the ecosystem of normal commensal bacteria and play a role in reviving a healthy microbial flora rather than eliminating them.

[0098] Also, regarding the skin, various microorganisms exist on its surface, and they play an important role in maintaining the balance of the skin. These microorganisms include bacteria, fungi, viruses, etc., and are usually called the "skin microbiota" or "skin flora". The skin microbiota contributes to various health functions such as regulating the immune system on the skin surface and preventing the invasion of pathogens. The skin commensal flora is an aggregate of microorganisms present on the skin, and its balance greatly affects the health of the skin. The main commensal bacteria include acne bacteria, Staphylococcus aureus, Staphylococcus epidermidis, etc.

[0099] On the other hand, if the number of bad bacteria increases too much, it may cause harmful problems for the skin. For example, Staphylococcus aureus is a bacterium that causes wounds to suppurate. It also causes dryness and has been pointed out to be related to atopy. Opportunistic bacteria perform a beauty-skin-like function in a healthy state, but when the balance is disrupted and the number of bad bacteria increases, they perform a bad-bacteria-like function. For example, acne bacteria usually play a role in enhancing the skin's moisturizing function, but when sebum increases, it appears as acne.

[0100] The oral mucosa, lips, and facial skin are located in the boundary region between the epidermis and the mucosa, and the microbiota in these regions are linked as an ecosystem, and microorganisms may move from one region to the other. This cooperation affects the health of the microbiota in each region.

[0101] The skin and oral cavity provide barriers to prevent the invasion of external pathogens. The microbiota is part of this barrier, and it has been suggested that commensal bacteria contribute to the immune response in the skin and oral cavity. The interaction between microorganisms and the host affects the barrier function.

[0102] There are different types of commensal bacteria in the oral mucosa, lips, and facial skin, each occupying a different biological niche. These microorganisms play roles such as redox reactions, supply of metabolites, and competitive exclusion of pathogens.

[0103] Understanding the communication of commensal bacteria may contribute to the development of future personalized medicine. By analyzing the microbiota profiles in the host's skin and oral cavity, optimal preventive measures and treatment methods can be provided for each individual. This approach can be a means to more effectively treat oral infections and skin problems.

[0104] The skin commensal flora is an aggregate of microorganisms present on the skin, and its balance greatly affects skin health. The main commensal bacteria include acne bacteria, Staphylococcus epidermidis, and Staphylococcus hominis. Staphylococcus epidermidis is a bacterium that does good things for the skin. It keeps the skin pH weakly acidic, produces moisturizing components, and produces components that suppress the growth of harmful bacteria, thus protecting the skin's barrier function. On the other hand, if harmful bacteria multiply too much, they may cause harmful problems to the skin. For example, Staphylococcus aureus is a bacterium that causes wounds to suppurate, causes skin dryness, and has also been pointed out to be related to atopy.

[0105] Oral commensal bacteria and skin commensal bacteria exist in different environments, but are related in many aspects such as the host's immune response, barrier function, immune regulation, biochemical interactions, and symbiotic relationships. These microbiota play important roles in maintaining the health and balance of the host. From a molecular and biochemical perspective, oral commensal bacteria and skin commensal bacteria affect the host's immune response in their respective environments. These microbiota interact with the host's immune system and are involved in the regulation of inflammation and infectious diseases. Certain microorganisms may stimulate the immune response, thereby suppressing or promoting inflammation.

[0106] The skin is the first point of contact with the outside of the body, and skin commensal bacteria help maintain its barrier function. Some skin commensal bacteria prevent the invasion of pathogens and contribute to maintaining skin health, and oral commensal bacteria also support the barrier function in the oral cavity. In addition, the microbiota in the skin and oral cavity play a role in regulating the host's immune system. This suppresses excessive immune responses and contributes to the prevention of autoimmune diseases and allergic reactions. On the other hand, it is known that when the balance of microorganisms is disrupted, the risk of inflammatory diseases increases.

[0107] Both the oral mucosa and the skin are composed of squamous epithelium, and the microbiota in their respective environments affect health. Considering the possibility that prebiotics for improving the oral environment may also be effective for the skin, the oral cavity and the skin provide similar environments for the growth of microorganisms. These microorganisms exist in both the skin and the oral cavity, and it has been demonstrated that certain types of microorganisms are adapted to both environments, and methods for improving the balance of microorganisms in the oral cavity may also be applicable to the skin.

[0108] The skin and oral microbiota are closely related to the immune response, and a healthy microbiota modulates the host's immune response and enhances defense against pathogens. It is thought that prebiotics that improve the oral environment may also have a beneficial effect on the immune response of the skin. The skin and oral cavity provide a physical barrier against the invasion of external pathogens, and a healthy microbiota complements these barrier functions and helps maintain the health of the skin and oral cavity. Improving the oral environment also contributes to supporting the skin barrier.

[0109] Regarding the development concept of the oral cosmetics according to the present invention, mainly the oral cavity will be described.

[0110] In the oral cavity, there are microorganisms in various forms such as cocci, bacilli, and filamentous bacteria, and about 700 species of bacteria are resident. Dental plaque is a mass of these bacteria, and it is said that there are 1 billion bacteria in 1 g. Nearly 100 million bacteria inhabit the tooth surface, periodontal pockets, tongue, etc., inducing various diseases such as tooth decay, periodontal disease, stomatitis, and halitosis. In the periodontal pockets of periodontal disease teeth, there are more than 1 billion bacteria living.

[0111] Roughly dividing the nearly 1 billion bacteria, they are classified into three types: beneficial bacteria, harmful bacteria, and opportunistic bacteria. Beneficial bacteria include lactic acid bacteria, Bifidobacterium that produces lactic acid and acetic acid, and Lachnococcus Coprococcus that produces acetic acid and butyric acid. Harmful bacteria include Mutans streptococci, which are the cause of tooth decay, and Porphyromonas gingivalis, which is the cause of periodontal disease. Opportunistic bacteria do not belong to either beneficial bacteria or harmful bacteria, but act according to the dominant side of beneficial bacteria and harmful bacteria. The ideal balance of this bacterial flora is said to be 90% beneficial bacteria including opportunistic bacteria and 10% harmful bacteria. In the oral cavity, there are beneficial bacteria (prebiotics) that condition the oral environment, but such an anti-periodontal disease oral composition with a high bactericidal effect may kill not only harmful bacteria but also beneficial bacteria. As a way to deal with oral care, it is not necessary to kill beneficial bacteria. As for the corresponding method of oral care, it is not necessary to kill beneficial bacteria.

[0112] It has been elucidated that major oral diseases such as dental caries, periodontal disease, and stomatitis occur due to the disruption of the balance of oral resident bacteria. Dental caries is mainly caused by mutans streptococci, which grow in plaque (dental biofilm) attached to the tooth surface. On the other hand, there are multiple causative bacteria for periodontal disease, which are different in type from mutans streptococci. These bacteria are also present in plaque, like mutans streptococci, but they have an anaerobic property and tend to grow deeper. Thus, dental caries and periodontal disease are caused by different bacteria respectively. When the bacterial balance in the oral cavity is disrupted, these bacteria are more likely to grow, increasing the risk of developing dental caries and periodontal disease. Maintaining an appropriate bacterial balance in the oral cavity can prevent dental caries and periodontal disease.

[0113] There are trillions of microorganisms symbiotically living in and on the human body. There are bacteria that perform beneficial functions for the human body (good bacteria) and those that cause harm (bad bacteria). What is important is not to "eliminate bacteria completely" but to increase good bacteria and create a balanced microbiota. Improving oral resident bacteria (intestinal bacteria in the intestine) is crucial for maintaining oral health. Resident bacteria symbiotic with the human body are said to have the effect of suppressing viruses that invade from the outside and preventing the aggravation of diseases.

[0114] The oral cosmetics of the present invention are developed with the aim of maintaining a good flora in the oral cavity and on the skin by adding plant minerals and EM bacteria to 100% natural materials and using prebiotics that promote the growth of good bacteria that have a beneficial effect on the human body. Clinical trials have shown effectiveness in preventing and improving gingivitis, periodontal disease, candidiasis, and bad breath. It is an oral cosmetic that takes into account overall health starting from the entrance of the body.

[0115] Herb components are known to have anti-inflammatory and antibacterial effects and help regulate the oral microbiota. In addition, vitamins and minerals play a role in maintaining the health of cells and supporting the regeneration and repair of oral mucosa. The synergistic effect of a combination of multiple types of natural herbal medicines produces an effect that simultaneously supports the balance of the oral resident flora and the health of the oral mucosa. The emollient effect occurs in maintaining the balance of the skin resident flora in weakly acidic skin, and it can also be used together as skin care for dryness due to aging and various skin troubles.

[0116] The Human Microbiome Project (HMP) initiated by the National Institutes of Health (NIH) in the United States has greatly contributed to deepening the understanding of health and disease and the development of new therapies. The effects of diet, oral environment, drug use, etc. on the microbiota have been elucidated in detail.

[0117] As a result, the relationship between the resident flora symbiotic with humans and health has become clear, the importance of adjusting the balance of the resident flora has been recognized, and probiotics (supplementing beneficial bacteria) and prebiotics (food components that promote the growth of beneficial bacteria) have undergone great evolution. In particular, it has been demonstrated that adjusting the balance of the gut microbiota leads to improved health. By adjusting the oral microbiota, great contributions are expected to the improvement of oral health and the prevention and treatment of diseases.

[0118] Understanding of the interactions and symbiotic relationships between microorganisms has advanced, and it has been elucidated how the oral microbiota affects each other and which microorganisms are involved in biofilm formation. It has become possible to design probiotics and prebiotics to control them. In particular, the evolution of prebiotics has also contributed to the development of new oral therapies, and by adjusting the balance of the microbiota, it has become possible to approach diseases that were difficult to treat with conventional therapies.

[0119] The development of prebiotics was carried out based on the following concepts.

[0120] First, the balance of the oral flora was achieved. In recent years, it has been elucidated that the main oral diseases such as dental caries, periodontal disease, and stomatitis occur due to the disruption of the balance of the oral resident bacteria. However, herbs formulated in the oral cosmetics of the present invention were carefully selected from about 200 documents, and oil and aromatic distilled water were produced by a low-temperature distillation extraction method that does not destroy the components. The herbs formulated in the oral cosmetics of the present invention have a synergistic effect proven by research, and thus can play the role of prebiotics for the oral resident flora.

[0121] The plant minerals of humic shale (fulvic acid) formulated in the oral cosmetics of the present invention are bound to organic substances such as organic acids and amino acids, have high hydrophobicity similar to cell lipids, and are easily incorporated into the lipid bilayer from the cell membrane. Therefore, due to the function of the transport proteins present in the cell membrane, the components can easily pass through the cell membrane, enhancing the effect and resulting in biological effects.

[0122] Also, no synthetic components such as preservatives and additives are used at all. Instead of preservatives and interfacial regulators such as phenoxyethanol, ethanol, and parabens used to suppress the growth and contamination of microorganisms contained in general cosmetics, the antibacterial and preservative effects of phytontid and hinokitiol are enhanced.

[0123] For example, commercially available oral rinses may disrupt the balance of the oral resident flora. Commercially available oral rinses may contain antibacterial agents and bactericidal components such as antioxidants, preservatives, and additives. Although these components are aimed at reducing bacteria in the oral cavity, they may also reduce beneficial bacteria and necessary bacteria at the same time, thereby disrupting the balance and deteriorating the oral health condition.

[0124] As prebiotic materials, the necessary conditions are shown below.

[0125] (1) The selected material for the prebiotic effect is preferably having a probiotic effect that promotes the growth of beneficial bacteria in the oral cavity.

[0126] (2) It is important that the antibacterial effect has the antibacterial effect of suppressing biofilm formation and preventing the growth of harmful bacteria.

[0127] (3) For the anti-inflammatory effect, a material with an anti-inflammatory effect that reduces inflammation and maintains the health of oral tissues is desirable. Natural-derived prebiotic materials are preferably natural-derived, and organic materials that have not been chemically processed are desirable.

[0128] (4) To achieve sustainability, it is important to select materials that use environmentally considerate sustainable cultivation and harvesting methods.

[0129] (5) Compatibility and combination are important, and it is necessary to examine whether a synergistic effect can be obtained when combining multiple materials.

[0130] (6) For the scientific basis of the effect, it is desirable that the effect of the selected material is scientifically supported.

[0131] (7) For compatibility with the product form, it is important to consider how to incorporate the prebiotic material into the product form and select an appropriate form.

[0132] (8) For long-term stability, it is required that the effect persists during the storage and use of the product.

[0133] (9) For scientific evaluation, it is necessary that the material undergoes scientific evaluation and its effect and safety are confirmed.

[0134] (10) For quality control, it is important to thoroughly implement quality control of the material and maintain consistent quality.

[0135] The selected material must have a probiotic effect that promotes the growth of beneficial bacteria in the oral cavity. And it is important to have an antibacterial effect that inhibits biofilm formation and prevents the growth of harmful bacteria. Furthermore, it is desirable to have an anti-inflammatory effect that reduces inflammation and maintains the health of oral tissues. For this reason, prebiotics materials are preferably of natural origin, and organic materials that are not chemically processed are desirable.

[0136] It is important to select materials that have been cultivated and harvested using sustainable methods that consider the environment, in order to enhance sustainability. It is also important to examine the compatibility and combination of multiple materials to see if a synergistic effect can be obtained when they are combined. It is desirable that the effects of the selected materials are chemically verified. Also, it is important to consider how to incorporate the prebiotics materials into various product forms and select an appropriate form, and it is required that the effects persist during storage and use. Furthermore, it is important that the materials undergo chemical evaluation, their effects and safety are confirmed, and quality control of the materials is thorough and consistent quality is maintained.

[0137] Explain the basis for improving the oral environment by the synergistic effect of the components of oral prebiotics with natural materials blended.

[0138] The herb components contained in prebiotics have properties such as antibacterial, anti-inflammatory, and antioxidant, and these components act synergistically to suppress the growth of harmful oral bacteria and reduce inflammation. Also, the aroma and flavor of the herb components also enhance the sense of oral cleanliness and contribute to maintaining a healthy oral environment.

[0139] Phytominerals contain calcium, magnesium, etc. that are important for the health of teeth and periodontal tissues. These minerals help promote the remineralization of tooth enamel and maintain the strength of teeth, and the presence of minerals has an effect of suppressing biofilm formation.

[0140] Vitamins are involved in strengthening the immune system and tissue repair. Since the oral mucosa is constantly stimulated, vitamin supplementation is important. By including vitamins in Prebiotex, it maintains the health of the oral mucosa, supports recovery from infections and injuries, and exhibits a complementary effect.

[0141] Fermented garlic contains antibacterial components such as allicin, which suppresses the growth of harmful bacteria in the oral cavity. EM (effective microorganisms) preparations also play a role in regulating the microbial balance in the oral cavity and promoting the increase of beneficial bacteria, thereby enabling the prevention of biofilm formation and the improvement of bad breath.

[0142] When these components are combined, a synergistic effect is exhibited. For example, specific synergy effects such as herb components suppressing harmful bacteria, vitamins enhancing immunity, and fermented garlic and EM agents regulating the microbial balance are manifested.

[0143] The effects that the components of Prebiotex exert on the oral cavity also affect the microbiota. The oral microbiota is closely related to the intestinal microbiota, and a healthy microbiota affects immune function and metabolism. Therefore, oral Prebiotex improves the balance of oral biofilms and microbiota, which has a beneficial effect on overall health. In addition, the components of Prebiotex regulate the immune response in the oral cavity, thereby suppressing excessive inflammatory responses and maintaining the health of the oral mucosa. Oral health has a beneficial effect on the body's immune system.

[0144] By changing the microbial interactions in the oral cavity, the components of prebiotics enhance the cooperative relationship between beneficial bacteria. As a result, the growth of harmful bacteria is suppressed, and oral health is maintained. Maintaining a healthy oral environment also affects overall health, and research has shown that oral inflammation and infections have a significant impact on the body and are associated with cardiovascular diseases and diabetes. By improving the oral environment, prebiotics also contribute to reducing the risk of these systemic diseases. Some of the components contained in prebiotics have antioxidant effects, which suppress oxidative stress and inflammatory reactions in the oral cavity and reduce the risk of gum diseases and stomatitis.

[0145] The minerals and vitamins contained in prebiotics promote the remineralization of tooth enamel, thereby increasing the strength of the tooth surface and reducing the risk of tooth decay. Some of the components contained in prebiotics have the effect of suppressing the growth of bacteria that cause bad breath. By improving the biofilm in the oral cavity, bad breath is reduced. Furthermore, since prebiotics contain components that support the health of the oral mucosa, damage and inflammation of the oral mucosa are prevented, and the risk of oral problems is reduced.

[0146] When the components of prebiotics have the effect of supporting the immune system, the immune response in the oral cavity is enhanced. As a result, the resistance to infections and inflammation in the oral cavity is improved, and a healthy state is maintained. When the components contained in prebiotics have the effect of adjusting the pH in the oral cavity, the enamel of the teeth is protected by improving the acidic environment, and the damage caused by acid is reduced.

[0147] When the components contained in prebiotics have the function of maintaining moisture in the oral cavity, relief of oral dryness symptoms is expected. By reducing dryness, the oral microbiota is kept healthy. When the components of prebiotics have the effect of improving the diversity of the oral microbiota, the presence of beneficial bacteria increases, and the growth of pathogenic bacteria is suppressed.

[0148] Herbal prebiotics are effective not only in balancing the oral microbiota but also in maintaining the health of the oral mucosa. Herbal prebiotics not only regulate the balance of the oral microbiota but also have a beneficial effect on the health of the oral mucosa. The oral microbiota is closely related to the oral mucosa, and these interactions play an important role in oral health.

[0149] Herbs contain bioactive components such as antibacterial and anti-inflammatory polyphenols, flavonoids, and terpenoids. These components have the effect of suppressing harmful bacteria and inflammation in the oral cavity and contribute to the improvement of the oral environment. In particular, the oral mucosa is highly sensitive to external stimuli and environmental changes and is prone to inflammation and damage. Therefore, the anti-inflammatory effect and protective action of herbs have a good impact on the whole body. Herbs have been carefully selected for their ability to balance the oral commensal bacteria. The following herbs will be described.

[0150] Chamomile has an anti-inflammatory effect and suppresses gum inflammation. Chamomile tea also has a mild antibacterial effect and helps maintain the balance in the oral cavity. Lavender has an antibacterial effect and suppresses the growth of harmful bacteria in the oral cavity. It also has a relaxing effect and reduces the impact of stress on the oral condition. Lemongrass has an antimicrobial effect and suppresses the growth of bacteria in the oral cavity. Its fresh scent also soothes bad breath. Sage has antioxidant and anti-inflammatory effects and supports gum health. Sage tea also helps maintain the balance of the oral environment. Rosemary has an antibacterial effect and suppresses the growth of bacteria in the oral cavity. The scent of rosemary also has a refreshing effect and has a good impact on the oral condition.

[0151] Oral commensal bacteria are a collection of microorganisms, Biofilm, that constantly exist in the oral cavity. These microorganisms form an ecosystem in the oral cavity and play various roles. Oral commensal bacteria play an important role in maintaining the balance of the oral ecosystem and supporting the healthy state. Since diseases such as tooth decay, periodontal disease, and periodontitis are caused by a disrupted balance, it is important to maintain a healthy balance of oral microorganisms.

[0152] Some of the resident bacteria in the oral cavity secrete digestive enzymes that assist in the decomposition of food and provide digestive support. As a result, food digestion is carried out efficiently, and nutrient absorption is promoted. In addition, oral resident bacteria are involved in the immune response and are known to regulate the immune system of host cells. Under an appropriate balance, they play a role in activating or suppressing the host's immune system.

[0153] Oral resident bacteria form a biofilm that adheres to the surface of teeth and plays a role in preventing the growth of bacteria that cause dental caries and periodontal disease through its barrier function of preventing the invasion of pathogens, thus maintaining dental health. Furthermore, since it can decompose food residues, it suppresses the occurrence of dental caries. It also has the function of suppressing bad breath caused by the activities of bacteria in the oral cavity. By controlling the formation of the biofilm, an effect of suppressing the generation of odor occurs. The biofilm adheres to the tooth surface to form plaque, but at the same time is also involved in the remineralization of teeth. The tiny spaces within the biofilm facilitate the attachment of minerals and have the effect of reversing the demineralization of enamel, which is the initial stage of dental caries. The healthy microorganisms present within the biofilm maintain oral health by competitively inhibiting the attachment and growth of pathogenic bacteria.

[0154] Oral resident bacteria play a role in maintaining the health status of host cells through their interaction with host cells. By regulating the immune response and metabolizing nutrients, they adjust the oral environment and support the health of the host. In addition, oral resident bacteria play a role in supplying nutrients to host cells by decomposing specific nutrients to produce metabolites. Some microorganisms contribute to the health of the host by producing vitamins and amino acids.

[0155] When the balance of oral resident bacteria is disrupted, the growth of pathogenic bacteria that cause periodontal disease progresses, leading to oral inflammation. A normal biofilm contributes to the suppression of inflammation and is an important factor in maintaining oral health. By maintaining a healthy biofilm, the risk of periodontal disease can be reduced.

[0156] Figure 1 is a conceptual diagram of the oral microbiota balance according to the present invention. The oral microbiota balance is achieved by the synergistic effect of the probiotic agent 12, which is a microbiota balance regulator, the plant mineral 14, the EM preparation 16 that enhances cell activation and maximizes the fermentation power, and the vitamin agent 18, against the antibacterial and bactericidal component 20 and the antioxidant component 22. Each component will be described below.

[0157] As the probiotic agent 12, the inventor has carefully selected materials from about 200 or more research papers and found that herbs are preferable. For the herbs, their extracts are used. There are many types of herbs, such as chamomile, lavender, lemongrass, sage herb, rosemary, Italian parsley, basil, lemongrass, coriander, thyme, mint, rosemary, laurel, oregano, shiso, chive, nasturtium, arugula, lemon verbena, and so on.

[0158] Among these herbs, chamomile, lavender, lemongrass, sage herb, or rosemary exhibit the function as a probiotic, and it is preferable to use these herbs.

[0159] It is preferable to extract the flowers of chamomile as chamomile oil for use.

[0160] It is preferable to extract the flowers of lavender as lavender oil for use.

[0161] It is preferable that lavender oil is extracted only from the flower buds of true lavender by natural cultivation.

[0162] It is preferable to extract at least one of the leaves and stems of lemongrass as lemongrass essential oil for use.

[0163] It is preferable that sage herb is extracted and used as sage herb essential oil from at least one of the leaves and flowers of sage herb.

[0164] It is preferable that rosemary is extracted and used as rosemary essential oil from at least one of the leaves and flowers of rosemary.

[0165] It is preferable that chamomile oil, lavender oil, lemongrass essential oil, sage herb essential oil, and rosemary essential oil are produced and extracted by a low-temperature distillation extraction method that does not destroy the components.

[0166] Here, the extraction by the low-temperature distillation extraction method will be explained using rosemary as an example. First, fresh and high-quality rosemary leaves and branches are harvested. To obtain high-quality oil and aromatic distilled water, it is important to select fresh and healthy rosemary. The distiller includes a flask for putting the raw material of rosemary and a distillation boiler for sending steam. Water is added to the distillation boiler and heated to generate steam, which is sent to the flask and brought into contact with the raw material of rosemary.

[0167] The oil and aromatic components contained in the raw material of rosemary dissolve in the steam and are collected in the flask in the form of oil and aromatic distilled water. The oil and aromatic distilled water accumulated in the flask are cooled, and the oil usually separates from the water and floats on top, while the aromatic distilled water sinks to the bottom. A separation device is used to take out the oil and aromatic distilled water to obtain rosemary oil and rosemary aromatic distilled water. Since the low-temperature extraction method extracts oil and aromatic distilled water from rosemary without applying heat, the components of the plant can be retained more efficiently.

[0168] Rosemary oil and distilled aromatic water mainly support oral health by promoting the growth and activation of beneficial bacteria in the oral cavity, suppressing the growth of harmful bacteria, functioning as prebiotics, helping to increase beneficial bacteria in the oral cavity, and contributing to the prevention of tooth decay and periodontal disease. These functions contribute to maintaining the balance of the bacterial flora.

[0169] The plant mineral 14 is preferably fulvic acid extracted from humic shale.

[0170] Humic shale, which is included in oral care products, is one of the components of oral care products and is a type of rock existing within the Earth's crust. Humic shale is mainly formed from organic matter. Humic shale is a rock mainly formed by the deposition of an organic substance called humic acid, and shale contains a high concentration of organic matter. Humic acid is a type of organic matter formed through the long-term decomposition of plant and microbial sediments and through chemical and physical changes, and it is a plant mineral accumulated since ancient times.

[0171] Fulvic acid can be extracted by pulverizing humic shale into fine powder and immersing it in water. Generally, it is carried out by the following process.

[0172] First, humic shale is mined from underground and pulverized to an appropriate particle size. By immersing the pulverized humic shale in water or a specific solvent, humic acid and fulvic acid are extracted. Heat or pressure may be applied for extraction. Impurities are removed from the extract, and humic acid and fulvic acid are concentrated. Methods such as evaporation, filtration, and purification may be used. The concentrated liquid is dried to obtain solid humic acid and fulvic acid, and by processing the solid humic acid and fulvic acid into powder form, the final product is obtained. It may also be made into a liquid form.

[0173] Fulvic acid, a plant-based mineral 14, is collected from humic shale in the United States and has a body absorption rate of about 98% as nano-sized particles. It is an energy source for biological activities and is known for preventing allergic reactions, reducing inflammation, preventing and suppressing cancer growth, and having antibacterial effects. It supports the functions of vitamins, enzymes, proteins, and the absorption of minerals necessary for tooth enamel. Among the minerals necessary for tooth enamel, there is calcium carbonate, which strengthens tooth enamel and removes dirt on the tooth surface. It is also known to have the effect of maintaining the pH balance of the skin. These functions contribute to maintaining the balance of the bacterial flora.

[0174] Humic shale contains more than 70 types of plant-based minerals. These plant-based minerals include lithium, beryllium, boron, carbon, sodium, magnesium, aluminum, silicon, phosphorus, sulfur, chlorine, potassium, calcium, scandium, titanium, vanadium, chromium, manganese, iron, nitrogen, nickel, copper, zinc, gallium, germanium, selenium, etc.

[0175] These 14 plant-based minerals are not metals but have changed into a form useful to humans by being absorbed and decomposed by plants. The plant-based minerals contained in fulvic acid have a chelating effect and a negative ionization with a nano-size (one billionth of a meter), resulting in a high body absorption rate of 98%. Since it is water-soluble and excess minerals are excreted, there is no worry about over-ingestion. Therefore, since humic shale can be extracted just by soaking it in water, it may be used as it is as a fine powder without extracting fulvic acid.

[0176] The EM agent 16 includes EM-X (Effective Microorganisms-X), which is a fermentation active ingredient. EM-X is an abbreviation for organic matter microbial fermentation extract and was developed by Professor Teruo Higa, an emeritus professor at the University of the Ryukyus. EM-X contains a mixed flora of microorganisms consisting of lactic acid bacteria, yeast, actinomycetes, etc., and these microorganisms bring beneficial effects in various fields.

[0177] EM-X is in the form of fine powder to maximize the power of fermentation so that the active ingredients can reach every corner of the body, and it has the function of regulating the immune balance. In addition, EM-X has antibacterial and anti-inflammatory effects. The microorganisms contained in EM-X promote the growth of beneficial bacteria in the oral cavity, thereby suppressing the growth of harmful bacteria and improving the balance of the oral flora. Also, the organic acids contained in EM-X contribute to the reduction of bad breath and dental plaque by suppressing the growth of harmful bacteria in the oral cavity, and the organic acids relieve inflammation in the oral cavity. These functions contribute to maintaining the balance of the flora.

[0178] Vitamin agent 18: Vitamin C and vitamin E are involved in strengthening the immune system and tissue repair, maintaining the health of cells, and playing a role in supporting the regeneration and repair of oral mucosa. Through the synergistic effect of combinations with various types of natural herbal medicines, it simultaneously supports the balance of the oral resident flora and the health of the oral mucosa. By including vitamins in prebiotics, it maintains the health of the oral mucosa, supports recovery from infections and injuries, and exerts a complementary effect.

[0179] Vitamin C (ascorbic acid) contributes to maintaining the health of the gums, preventing bleeding and swelling of the gums, and also contributes to the prevention of periodontal disease. It also has an antioxidant effect and reduces inflammation in the mouth. Vitamin D helps with calcium absorption and strengthens the tooth bone. Vitamin A contributes to maintaining the health of tooth enamel and mucosa. It plays a role in protecting the mucosa in the oral cavity and maintaining healthy gums. Vitamin E protects cells in the mouth and has an antioxidant effect, thus helping to keep the tissues in the oral cavity healthy. Among the B vitamins, vitamin B1, B2, and B6 are particularly suitable. They activate the metabolism of the skin and mucosa and are effective against stomatitis.

[0180] Combining these ingredients, namely herbal oil, fulvic acid, EM-X, and vitamins, brings about a synergistic effect. In particular, the combination of these ingredients optimizes the balance of the oral flora and is effective in preventing tooth decay, periodontal disease, etc.

[0181] In the embodiments of the invention, oral cosmetics, by formulating these active ingredients in an optimal ratio, maintain oral health and contribute to disease prevention. Such oral cosmetics, when used as part of daily care, can maintain oral and skin health in the long term.

[0182] Based on the above description, the present invention provides a new option for oral care in the daily lives of many people as a new approach to maintaining and improving oral and skin health.

[0183] Furthermore, more effective oral cosmetics are considered by incorporating recent research results. The development of prebiotics for oral commensal bacteria requires a lot of research, including the discovery of new ingredients and the optimization of effects. Research on prebiotics using crude drugs and natural materials is progressing, and in particular, the combination of herb-based essential oils, plant minerals, and vitamins has attracted attention.

[0184] Assistant Professor Tetsuhito Sakana, Associate Professor Masae Kubaba, Professor Atsuo Amano of the Graduate School of Dentistry, Osaka University, Associate Professor Shuuichi Niima, Professor Eiichiro Fukazawa of the Graduate School of Engineering, Osaka University, through an international joint research with the University of Louisville (Kentucky, USA), clarified the pathogenesis mechanism of periodontal disease through the bacterial nutritional network centered on Fusobacterium nucleatum in oral biofilms.

[0185] F. nucleatum, an oral commensal bacterium, obtains energy using amino acids, which are excreta for good bacteria, and uses it to produce polyamines to nourish periodontal pathogens, thus promoting the maturation and high pathogenicity of oral biofilms. From this, it is considered that if oral hygiene management is neglected, F. nucleatum will act as a nutritional bridge, and bacteria in the oral biofilm will spontaneously start exchanging metabolites, ultimately leading to an increase in bad bacteria. If further research progresses in the future and the bacterial nutritional network in the oral biofilm can be controlled, a new method for preventing periodontal disease can be developed.

[0186] It has been suggested that the mechanism of periodontal disease development is mediated by a bacterial nutritional network. Specifically, metabolome and gene expression analysis of F. nucleatum when co-cultured with S. gordonii revealed that F. nucleatum increased the expression of an enzyme called ornithine decarboxylase (FN0501) and metabolized ornithine provided by S. gordonii to putrescine. In addition, when co-cultured with an ArcD-deficient strain of S. gordonii, putrescine production by F. nucleatum was significantly reduced. This indicates that F. nucleatum produces putrescine dependent on the supply of ornithine via S. gordonii ArcD.

[0187] A research group led by Professor Yoshihiko Tanaka (who also serves as leader of the Oral Medical Research Center) and Lecturer Junichi Nagao of the Department of Infection Biology, Faculty of Oral and Dental Science, Fukuoka Dental College, has clarified the mechanism by which helper T cells, which are influenced by intestinal bacteria and begin to respond to periodontal pathogens in the intestines, are involved in the onset and aggravation of periodontal disease.

[0188] When the amount of periodontal pathogenic bacteria that periodontal disease patients swallow daily is introduced into the intestines of mice, the periodontal pathogenic bacteria are taken up through the intestinal Peyer's patches, and Th17 cells (responsible Th17 cells) that respond to the periodontal pathogenic bacteria are activated in the intestines. The responsible Th17 cells then move from the intestines to the gums infected with the periodontal pathogenic bacteria, causing the onset and aggravation of periodontal disease. On the other hand, in germ-free mice (without intestinal bacteria), the responsible Th17 cells were not activated and periodontal disease did not occur, proving that intestinal bacteria are involved in the onset of periodontal disease. It was also found that when the intestinal bacteria of mice are disturbed by certain antibiotics, the activation of the responsible Th17 cells is enhanced and periodontal disease becomes more severe. Furthermore, it was also found that there are antibiotics that conversely suppress the onset of periodontal disease. A new method of preventing periodontal disease using drugs that target intestinal bacteria is possible.

[0189] In order to incorporate the research results of Osaka University and Fukuoka Dental College mentioned above, oral cosmetics were investigated.

[0190] The bacterial trophic network refers to the state where different species of bacteria and microorganisms provide nutrients to each other and circulate within the ecosystem. In this network, certain bacteria may secrete specific substances, which are then utilized by other bacteria to obtain nutrients. Conversely, the original bacteria may also benefit by using substances secreted by other bacteria. Such trophic interactions play a role in optimizing the utilization and distribution of resources within the ecosystem, and the bacterial trophic network is thought to be involved in maintaining biodiversity and ecosystem stability.

[0191] F. nucleatum (Fusobacterium nucleatum) present in the oral biofilm is an indigenous oral bacterium associated with periodontal disease. Since the bacterial trophic network centered around F. nucleatum is involved in the onset of periodontal disease, in order to control the bacterial trophic network, it is necessary to maintain the balance of the bacterial flora and suppress the accumulation of dental plaque.

[0192] To control the bacterial trophic network within the oral biofilm, the use of prebiotics and dental plaque control agents are effective. Examples of common prebiotics include lactic acid bacteria (such as Lactobacillus and Bifidobacterium) and yeasts (such as Saccharomyces boulardii). By including prebiotics, it has the effect of balancing the oral microbiota and reducing the risk of oral problems such as tooth decay and periodontal disease. Lactic acid bacteria, Lactobacillus and plant-derived Bifidobacterium, and Saccharomyces boulardii are increased in number by culturing and mixed into dentifrices in an active state. Examples of dental plaque control agents include fluoride. Fluoride strengthens the tooth surface and has the effect of preventing the dissolution of teeth by acids.

[0193] F. nucleatum is part of the normal oral flora present in the oral cavity. This bacterium is a Gram-negative bacterium and has a spindle shape. Antibiotics that suppress F. nucleatum include metronidazole and clarithromycin, but there are also issues of resistance and the risk of side effects such as nausea and vomiting in some people, and further research is still needed.

[0194] Plant antibiotics have the effect of suppressing F. nucleatum, including tea tree oil, clove oil, chamomile, phytoalexin, etc. Tea Tree Oil is a natural essential oil extracted from the leaves of the tea tree, which is a tree of the Myrtaceae family. Clove oil is an essential oil extracted from the flower buds of cloves (scientific name: Syzygium aromaticum), which are widely known as a spice. Chamomile is a plant of the Asteraceae family, and its flowers and flower buds are traditionally known for their medicinal use. It is mainly native to Europe and West Asia, with various types existing, and Roman chamomile and German chamomile are well-known.

[0195] Chamomile is safe, and its main active ingredients include bisabolol, chamazulene acid, flavonoids, etc. Phytoalexin is a chemical substance emitted by trees and other plants and is a volatile substance with bactericidal power. Although phytoalexin extracts are commercially available (for example, product name: PT-150 Crysxl0). There is also a commercially available microbial culture extract (product name: EM-X GOLD).

[0196] The nutrient network centered on F. nucleatum is important for maintaining the balance of the oral microbiota. When the oral environment deteriorates, harmful bacteria increase, the balance of the microbiota is disrupted, and anaerobic bacteria (harmful bacteria) in the periodontal pocket proliferate, periodontitis and periodontal diseases progress, and in the worst case, teeth are lost. In addition, dental plaque is a sticky film-like biofilm formed on the surface of teeth, which is formed by a mixture of bacteria in the oral cavity, food residues, proteins in saliva, etc. Dental plaque adheres to the surface of teeth and the junction between teeth and gums, and also causes dental calculus. Suppressing dental plaque is also important for maintaining the balance of the oral microbiota together with F. nucleatum.

[0197] Substances having the function as a gut microbiota regulator further include fulvic acid, garlic extract, mugwort extract, true lavender, plant secondary metabolites, microbial culture extracts of useful microbiota, etc. The gut microbiota regulator maintains the balance of the oral microbiota together with its antibacterial action. True lavender has analgesic, sedative, nerve-strengthening, and anti-inflammatory effects and is also used for the treatment of oral ulcers. In recent research, borneol contained in true lavender has a mechanism (blood-brain barrier BBB) that can only pass through substances limited to the entrance of the brain among compounds of monoterpene alcohol, but borneol has attracted attention as an aroma substance that opens that door. The content of borneol varies depending on the variety of lavender and cultivation conditions.

[0198] Borneol has medicinal effects on periodontal disease, such as antibacterial action, anti-inflammatory action, blood circulation promoting action, antioxidant action, etc. The antibacterial action of borneol controls the growth of pathogenic bacteria and suppresses the progression of periodontal disease. Borneol also has an anti-inflammatory action and suppresses the inflammatory reaction in the oral cavity. The anti-inflammatory action of borneol reduces the inflammation of periodontal tissues and prevents the progression of the disease. The blood circulation promoting action of borneol has the effect of improving the poor blood circulation of the gums due to periodontal disease. By promoting blood circulation, the supply of oxygen and nutrients to periodontal tissues increases, assisting in tissue regeneration and repair. Borneol also has an antioxidant action and has the effect of reducing oxidative stress in the oral cavity. Oxidative stress is involved in the progression of periodontal disease, and the antioxidant action of borneol suppresses the progression of periodontal disease.

[0199] Th17 cells that respond to periodontal pathogenic bacteria integrate cytokines of both innate and adaptive immunity and are involved in tissue inflammation and induce autoimmune phenomena, in contrast to Th1 and Th2 cells. Specifically, by inducing neutrophil infiltration and survival, matrix destruction, and production of pro-inflammatory cytokines by tissue-resident cells, it induces many inflammatory diseases. At the same time, it is said to play a role in the defense function of the living body against bacteria and fungi. Th17 cells play a role particularly in host defense against extracellular pathogens at mucosal and epithelial barriers, but abnormal activation is associated with the pathogenesis of various autoimmune diseases.

[0200] Substances having the function of preventing the activation of Th17 cells for periodontal disease prevention include fluoride, antibacterial components, anti-inflammatory components, antioxidant components, etc. Fluoride uses fluoride compounds (such as sodium fluoride and sodium monofluorophosphate), which can strengthen tooth enamel and prevent tooth dissolution by acids. Antibacterial components can inhibit the growth of dental plaque and oral bacteria, and have the effect of preventing the progression of periodontal disease. Representative antibacterial components include triclosan and chlorhexidine, but they are cytotoxic and continuous use is prohibited. Anti-inflammatory components can suppress inflammation, reduce gingival inflammation, and promote the health of periodontal tissues. Representative anti-inflammatory components include aloe vera, vitamin E, glycyrrhizic acid, etc. Antioxidant components can protect cells from oxidative stress and reduce the inflammatory response. Representative antioxidant components include vitamin C, vitamin E, green tea extract, etc.

[0201] Among them, antibacterial components can inhibit the growth of dental plaque and oral bacteria, and have the effect of preventing the progression of periodontal disease. In particular, tannins or their hydrolyzates are effective as additives that inhibit phosphatase activity. By combining this tannin or its hydrolyzate with a high-molecular compound having condensed phosphate or phosphate ester, the phosphatase activity in the oral cavity is inhibited, and the decomposition of condensed phosphate or phosphate ester into orthophosphate is prevented, and the effect of suppressing dental calculus, in which dental plaque hardens and mineralizes over time to form a solid crystal mass, is exerted over a long period.

[0202] Examples of compositions that act on the dopamine receptors of Th1 cells or Th17 cells of activated Th cells and can control the functions of these cells include tannic acid, gallic acid, and gallic acid esters of catechins, etc., which have agonist activity against dopamine D2 receptors. Dopamine D2-like receptor agonists in the cytokine production of each Th cell act on the dopamine D2-like receptors in activated Th cells and suppress the production of interleukin 8 (IL-8), an inflammatory mediator known as a neutrophil chemotactic factor. And dopamine D2-like receptor agonists have the effect of treating or improving periodontal disease. Dopamine D2-like receptor agonists are tannic acid, gallic acid, catechins or their gallic acid esters, ropinirole, pramipexole, etc.

[0203] Tannins are contained in persimmon tannin and lavender and are generally known as tannic acid. Persimmon tannin contains a large amount of tannins. Also, lavender contains tannins such as catechins and gallotannins. These tannins contained in lavender are present in the flowers, leaves, and stems of lavender and play a role in protecting the plant tissues, but the tannin content varies depending on the variety and cultivation conditions of lavender.

[0204] Regarding lavender containing borneol and tannins, extensive research has been conducted on the variety and cultivation conditions. As a result, it was found that the variety is true lavender, and it is particularly effective to use only the flower buds of true lavender before flowering. Also, it was found that the cultivation conditions are preferably natural cultivation that does not depend on pesticides. And only the flower buds of naturally cultivated true lavender were distilled to obtain lavender floral water.

[0205] The production of lavender floral water first uses a rare material obtained by distilling only the flower buds of authentic lavender grown naturally in EM (useful microorganisms) soil that does not rely on pesticides. The volatile oil components pass through the lavender and are cooled in a cooling tank together with the steam that has passed through, and come out in a state mixed with distilled water. Then, it is separated into oil and aromatic distilled water in an oil-water separation tank to obtain lavender oil and lavender aromatic distilled water.

[0206] The lavender aromatic distilled water produced in this way is saturated distilled water in which the volatile oil components are dissolved. The analysis results are 1,4-Cineol, 1,8-Cineole, 5-Methyl-3-heptanone, Octen-l-ol, acetate, Hexyl formate, 3-Octanol, Camphor, Linalool acetate, Borneol, alpha.-Terpineol, alpha.-Methyl-.alpha.-[4-methyl-3-pentenyl]oxiranemethanol, (-)-4-Terpineol, cis-3-Hexeny iso-butyrate, 6,6-Dimethyl-2-(3-oxobutyl)bicyclo[3.1.1]heptan-3-one.

[0207] To keep the oral cavity healthy, it is important to maintain the balance of the oral microflora. When the balance of the oral microflora deteriorates due to the deterioration of the oral environment, the number of harmful bacteria increases, the balance of the microflora is disrupted, and anaerobic bacteria (harmful bacteria) in the periodontal pocket proliferate, gingivitis and periodontal disease progress, and in the worst case, teeth are lost. Based on the latest research results, the present invention aims to provide oral cosmetics that can prevent dental caries, gingivitis, periodontitis, etc. and are stable for long-term preservation by maintaining a good balance of the oral microflora, and has studied oral cosmetics that are gentle to the human body by carefully selecting natural materials.

[0208] Figure 2 is a diagram for explaining the concept B of the balance adjustment of the microflora in the present invention. Incorporates the recently studied bacterial nutrient network control component 24 and Th17 fat activation prevention component 26 with respect to the concept A of the balance adjustment of the microflora in Figure 1.

[0209] First, regarding the inter-bacterial nutritional network control component 24, as described above, there are probiotics such as lactic acid bacteria (Lactobacillus, Bifidobacterium, etc.) and yeast (Saccharomyces boulardii, etc.). The lactic acid bacteria may be sodium lactate, and its antibacterial action can suppress the growth of bacteria that cause periodontal disease. Sodium lactate can suppress the formation of dental plaque and prevent the progression of periodontal disease. In addition, sodium lactate also has the effect of alleviating gingival inflammation.

[0210] For the Th17 cell activation prevention component 26, antibacterial components, anti-inflammatory components, and antioxidant components are effective. As compositions that can act on the dopamine receptors of Th1 cells or Th17 among activated Th cells and control the functions of those cells, there are tannic acid, gallic acid, and gallic acid esters of catechins, etc.

[0211] Based on the above considerations, in addition to the elements of the bacterial flora balance adjustment A, a new oral cosmetic containing the inter-bacterial nutritional network control component 24 and the Th17 cell activation prevention component 26 was created.

[0212] The oral cosmetic contains, in addition to a base, a bactericide and antibacterial agent, and a bacterial flora balance regulator, the inter-bacterial nutritional network control component 24 and the Th17 cell activation prevention component 26.

[0213] The base of the oral cosmetic has wetting agents, binders, preservatives, and antioxidants as basic components, and fragrances and sweeteners are added. The wetting agent gives the oral cosmetic appropriate moisture and plasticity. The binder binds the powder and liquid components and gives shape retention and appropriate viscosity. The fragrance agent aims to harmonize the fragrance, gives a refreshing feeling and aroma, and makes it easy to use. In addition, the oral disease prevention and treatment are achieved by the pharmacological action of the aromatic molecules of the plant extracts that make up this agent. Other ingredients such as sweeteners, cooling agents, and coloring agents may also be contained.

[0214] Examples of the wetting agent include glycerin, sorbitol, ethylene glycol, propylene glycol, polyethylene glycol, polypropylene glycol, butylene glycol, maltitol, lactitol, erythritol, pentylene glycol, hexylene glycol, octylene glycol, 1,3-butylene glycol, glycerin, and the like.

[0215] Examples of the binder include sodium carboxymethyl cellulose, methyl cellulose, hydroxymethyl cellulose, sodium alginate, xanthan gum, tragacanth gum, karaya gum, gum arabic, sodium polyacrylate, carboxyvinyl polymer, veegum, laponite, and the like.

[0216] Examples of the preservative include sodium benzoate and parabens. Examples of the antiseptic include butyl paraben, ethyl paraben, paraoxybenzoic acid ester, sodium benzoate, and the like. However, in this agent, plant extracts such as hinokitiol, persimmon tannin, and phytochitin perform that role. Examples of the sweetener include xylitol, sodium saccharin, aspartame, stevioside, stevia extract, paramethoxysinnamic aldehyde, neohesperidyl dihydrochalcone, perillartine, and the like. Note that this agent is an oral cosmetic that does not contain an abrasive.

[0217] Furthermore, a fragrance agent may be added. Examples of the fragrance agent include menthol, anethole, carvone, eugenol, limonene, n-decyl alcohol, citronellol, α-terpineol, citronellyl acetate, cineol, linalool, ethyl linalool, vanillin, thymol, spearmint oil, peppermint oil, lemon oil, orange oil, sage oil, rosemary oil, cinnamon oil, pimento oil, bay leaf oil, perilla oil, wintergreen oil, clove oil, eucalyptus oil, and the like. In addition, this agent contains lavender floral water and lemongrass floral water.

[0218] The bactericidal / antibacterial component 20, the antioxidant component 26, the bacterial inter-nutritional network control component 24, and the Th17 cell activation prevention component 26 are not substances that can be classified into substances with respective functions. Instead, one substance can have both a bactericidal function and the function of the bacterial inter-nutritional network control component. Therefore, by examining the functions of specific substances, it is possible to comprehensively achieve the balance adjustment B of the flora shown in Fig. 2.

[0219] The bactericidal / antibacterial agent added for the purpose of suppressing and antibacterial against harmful bacteria such as mutans bacteria which are the cause of dental caries and Porphyromonas gingivalis bacteria which are the cause of periodontal disease is preferably a plant-derived bactericidal / antibacterial agent without cytotoxicity to the living body. For example, there are hinokitiol, persimmon tannin, aged garlic, lemongrass, mugwort extract, and the like.

[0220] The chemical structural formula of hinokitiol is C 10 H 12 O2, which is an unsaturated seven-membered ring compound (monocyclic monoterpene) discovered for the first time and is one of the aromatic compounds. Hinokitiol was discovered in 1936 from the essential oil of Taiwan cypress growing wild in Taiwan by an associate professor who had come to Taiwan Imperial University. It has been applied as a dental therapeutic agent since ancient times.

[0221] Hinokitiol has a bactericidal action and a tissue astringent action, and is widely used by being formulated into oral compositions such as toothpaste for the purpose of alleviating various symptoms in gingivitis or periodontal disease such as bleeding, swelling, pain, gumminess, and bad breath, and treating stomatitis.

[0222] In addition, in this component, hinokitiol also serves as a deterioration inhibitor. However, since components such as lavender and lemongrass are recognized as fragrances, it is not a quasi-drug under the Pharmaceutical Affairs Law but is classified as a cosmetic. Therefore, the blending amount of hinokitiol is adjusted according to the cosmetic standards, and phytochitin is blended and used together.

[0223] Thujaplicin is contained in Taiwan cypress, Aomori hinoki, and Western red cedar produced in North America. Various methods have been conventionally known as the production method of thujaplicin, but regarding the extraction and generation of thujaplicin, there are a method of extracting from natural hinoki oil and a method of chemically synthesizing from dicyclopentadiene or the like. Regarding natural thujaplicin, the one with the highest content is Aomori hinoki, which is about 0.006%. When extracting thujaplicin from hinoki oil, about 10 kg of hinoki oil can be obtained from 1 t of lumber, and about 100 g, which is about 1% of it, is thujaplicin.

[0224] A method of chemically synthesizing thujaplicin that can be stably produced at low cost is known, which uses cyclopentadiene that is easily available as a raw material to obtain isopropylcyclopentadiene, adds dichloroketene to this, and obtains it by solvolyzing this adduct. Isopropylcyclopentadiene consists of three isomers, 1-isopropylcyclopentadiene, 2-isopropylcyclopentadiene, and 5-isopropylcyclopentadiene, but thujaplicin is known to be produced only from 1-isopropylcyclopentadiene.

[0225] Persimmon tannin, which contains a large amount of tannic acid, is a kind of polyphenol known to have antioxidant and antibacterial effects. Its molecular weight is about 11,000 to 15,000, which is a high molecular weight, and it has many phenolic hydroxyl groups (-OH) as functional groups. Persimmon tannin has a strong binding force with protein components, so it strongly binds to the protein components contained in the cell membrane of pathogenic bacteria, making it easy to adsorb pathogenic bacteria and showing a strong antibacterial effect and a hemostatic effect.

[0226] Persimmon tannin is extracted from, for example, astringent persimmons. The immature fruits of astringent persimmons are crushed, an appropriate amount of water is added, and then fermented. The fruit components such as sugar and pectin that strongly adsorb persimmon tannin during crushing are decomposed by fermenting yeast to purify persimmon tannin. It often takes one to three years or more to obtain a high concentration of persimmon tannin. For this reason, there is also a method of fractionating and precipitating with alcohol, dipotassium phosphate, and alkaloids to recover a high-concentration persimmon tannin component.

[0227] Antioxidants include ascorbyl tetra-2-hexyldecanoate, d-δ-tocopherol, tocopherol, ethanol, dipotassium glycyrrhizinate, vitamin C (ascorbic acid), vitamin E (tocopherol), etc.

[0228] It has been demonstrated that lemongrass has high antibacterial properties against oral microorganisms and an inhibitory effect on biofilm formation. The contents of geranial and neral, which are aldehydes in lemongrass, are higher than those in other plant essential oils. The antibacterial effect is at a concentration of 1 / 185 of the concentration of ethanol used for disinfection, and the antibacterial effect is remarkable compared to disinfectants.

[0229] Also, in clinical trials of bad breath suppression effect, the lemongrass preparation of natural ingredients showed higher results than the products using chemicals. The production of lemongrass aromatic distilled water is the same as that of lavender floral water.

[0230] The aged garlic extract contains sulfur-containing amino acids called allicin, alliine, methiin, and cycloalliine, peptides called gamma-glutamyl-S-allylcysteine and gamma-glutamyl-S-1-propenylcysteine, and a complex substance of a steroid compound called furostanol saponin and sugar. Garlic extract has the effect of improving the blood condition, increasing the number of good cholesterol, and decreasing the number of bad cholesterol. By ingesting garlic extract, the state of fat contained in the blood is improved. It also has a strong antioxidant effect and repairs oxidative damage caused by bad cholesterol. Garlic extract is also called a natural antibiotic, and the antibacterial effect of allicin contained in garlic is effective in improving and reducing gingivitis, stopping gingival bleeding, and has an effect on periodontal disease.

[0231] Yomogi extract has been used as a traditional Chinese medicine for over 2,000 years. It has anti-inflammatory effects by inhibiting the release of vitamins, astringent effects, anti-aging effects by reducing AGEs, and deodorizing effects. In addition, it has medicinal effects such as antipyretic effects, choleretic effects, antibacterial effects, antiviral effects, antifungal effects, lipid-lowering effects, and antihypertensive effects. The active ingredients of yomogi extract include tannins, flavonoids, essential oils (such as β-pinene and capillene), etc., and these ingredients are particularly effective in anti-inflammation and antibacterial.

[0232] Secondary metabolites of plants include cedar extract, hinoki extract, asanaro extract, momi extract, bamboo extract, maple extract, kunugi extract, ginkgo extract, birch extract, bamboo leaf extract, pine extract, oyster leaf extract, cherry leaf extract, nanten extract, mulberry extract, kusunoki extract, eucalyptus extract, kiri extract, loquat extract, sunflower seed extract, quince leaf extract, butterbur leaf extract, butterbur stem extract, tea extract, hop extract, chamomile extract, yomogi extract, dokudami extract, yuzu extract, lemon peel oil extract, etc.

[0233] As secondary metabolites of plants, there are phytoanticides, which have bactericidal and antioxidant properties due to their action of suppressing the activities of microorganisms. Phytoanticides are an example of microbial culture extracts, and many components with bactericidal power are contained in terpenoids contained in the essential oils of plants, including α-cadinol, camphor, citral, thymol, terpineol, borneol, limonene, etc.

[0234] Contrary to the definition that primary metabolites of plants are essential compounds in cell growth, development, and reproduction of plant bodies, secondary metabolites are defined otherwise and are compounds necessary for survival in nature. Many of them have biological activities and often play important roles in metabolic stress abnormalities under nutrient-deficient conditions. Phytoanticides, in addition to bactericidal, anti-inflammatory, and antifungal effects, have the function of normalizing cell metabolism together with preservative effects and calming effects, and are effective in maintaining the balance of the oral bacterial flora.

[0235] The microbial culture extract maximizes the power of fermentation and decomposes useful components into sizes that can reach every corner of the body, and has the effect of maintaining the balance of the oral bacterial flora. The microbial culture extract is centered around three types of beneficial bacteria, photosynthetic bacteria, lactic acid bacteria, and yeast, which repeatedly ferment and the bacteria break down the components finely and deliver them to every corner of the oral cavity.

[0236] Furthermore, it is preferable that the microbial culture extract contains crude magnesium chloride seawater and coral calcium.

[0237] Lavender is used as lavender floral water by the inventor's manufacturing method and is obtained by distilling only the flower buds before flowering of true lavender. Specifically, English lavender is used as the true lavender. True lavender is in a component system containing a sedative component called linalyl acetate, and in addition to borneol and tannins, it contains linalool, α-terpineol, coumarin, terpinene-4-ol, geraniol, etc. as components.

[0238] Figure 3 is a flowchart showing a manufacturing method 30 of lavender floral water obtained by distilling only the flower buds of naturally cultivated true lavender. First, as step S1, natural cultivation S1 of true lavender is performed. The contents of borneol and tannins vary depending on the variety and cultivation conditions of lavender. Therefore, English lavender is used as the true lavender. And it is grown by natural cultivation without using any pesticides. Next, as step S2, collection of flower buds before flowering is performed. Generally, lavender is harvested after the flowers have bloomed, but by collecting at the flower bud stage, the contents of borneol and tannins are rich and the medicinal effect is enhanced.

[0239] Next, in step S3, the harvested true lavender flower buds are placed in a distiller, and steam distillation is performed by a low-temperature extraction method. By placing the true lavender flower buds in the distiller and passing steam through them, the essential oil of the flower buds is extracted. The essential oil rises together with the steam and is collected as a liquid by being cooled. The distilled liquid is in a state where lavender essential oil and moisture are mixed. This liquid is cooled, and in step S4, the lavender essential oil and moisture are separated S4. The separated moisture becomes lavender floral water.

[0240] The separated lavender floral water contains minute remains of flower buds and impurities. In order to improve the quality, in step S5, the lavender floral water is filtered S5 to remove these impurities, and in step S6, the lavender floral water is extracted. Thereby, lavender floral water is obtained. The lavender floral water thus obtained contains borneol as a bacterial flora balance regulator and tannic acid that can act on the dopamine receptors of Th1 cells or Th17 of activated Th cells and control the functions of those cells, and has the medicinal effect of controlling the inter-bacterial nutrient network and preventing Th17 cell activation.

[0241] Components for preventing Th17 cell activation 26 include antibacterial components, anti-inflammatory components, and antioxidant components. Compositions that can act on the dopamine receptors of Th1 cells or Th17 of activated Th cells and control the functions of those cells include tannic acid, gallic acid, and gallic acid esters of catechins, etc.

[0242] The bacterial nutritional network control component 24 includes prebiotics, dental plaque control agents, and bacterial flora balance regulators. Prebiotics include lactic acid bacteria (such as Lactobacillus and Bifidobacterium) and yeasts (such as Saccharomyces boulardii). Dental plaque control agents include fluorine and plant antibiotics. Plant antibiotics include tea tree oil, clove oil, and chamomile. Bacterial flora balance regulators include fulvic acid, garlic extract, mugwort extract, true lavender, secondary metabolites of plants, and microbial culture extracts of useful microbial groups, etc.

[0243] Lactic acid bacteria are a general term for bacteria that obtain energy through the reaction of decomposing sugar to produce lactic acid. They include various species across many genera. They are cocci or bacilli and are Gram-positive. A typical example is Bifidobacterium. They exist in the oral cavity with sugars. Lactic acid bacteria may be sodium lactate, and due to its antibacterial effect, it can inhibit the growth of bacteria that cause periodontal disease. Sodium lactate can inhibit the formation of dental plaque and prevent the progression of periodontal disease. Also, sodium lactate can relieve gum inflammation.

[0244] Yeasts are clusters of single-celled living fungi belonging to the fungi category and are used in the production of bread, beer, and wine brewing. The cells are oval and reproduce by budding. They contain a lot of protein and are rich in vitamins and enzymes.

[0245] Dental plaque control agents include plant antibiotics, and plant antibiotics include tea tree oil, clove oil, and chamomile. Tea tree oil is a natural essential oil extracted from the leaves of the tea tree, which is a tree of the Myrtaceae family, and has properties such as antibacterial, anti-inflammatory, and antifungal. Clove oil is rich in a component called eugenol and has analgesic and antibacterial effects. It can also be used for bad breath prevention to prevent the growth of oral bacteria and for relieving throat inflammation. Chamomile has sedative, analgesic, and anti-inflammatory effects, as well as moisturizing and relaxation effects.

[0246] The microbiota balance regulator consists of herbal oil, fulvic acid of plant minerals, EM-X, vitamin C, and vitamin E. These components regulate the microbiota balance through their synergistic effects.

[0247] For the herbal oil, rosemary oil and rosemary aromatic distilled water extracted from rosemary by the low-temperature distillation extraction method are suitable. The rosemary oil extracted by the low-temperature distillation extraction method can retain the components of the plant more efficiently and functions as a prebiotic.

[0248] Rosemary oil and distilled aromatic water mainly promote the growth and activation of beneficial bacteria in the oral cavity, suppress the growth of harmful bacteria, support oral health as a prebiotic, help increase the beneficial bacteria in the oral cavity, and contribute to the prevention of dental caries and periodontal diseases. These functions contribute to maintaining the microbiota balance.

[0249] As the plant mineral fulvic acid, fulvic acid extracted from humic shale is suitable. With nano-sized particles, the in-vivo absorption rate is about 98%, which supports the functions of vitamins, enzymes, proteins, and the absorption of minerals necessary for tooth enamel.

[0250] For EM-X, EM-X Gold (registered trademark) sold as a product by EM Research Institute Co., Ltd. is used.

[0251] Figure 4 is a diagram showing Synergy Cycle 40 by the specific components of oral cosmetics based on the above examination results. The microbiota balance regulator consists of rosemary oil and distilled aromatic water 42, fulvic acid 46, vitamin C 52, vitamin E 54, and EM-X Gold 46, and the microbiota balance is adjusted by the interaction of these components combined.

[0252] The antibacterial and bactericidal component 20, antioxidant component 22, bacterial inter-nutritional network control component 24, and Th17 cell activation prevention component 26 consider the following points of the corresponding substances.

[0253] The plant-derived bactericidal and antibacterial components are phytic acid 48, hinokitiol 50, lemongrass 64, aged garlic extract 58, and mugwort extract 60. The antioxidants are vitamin C 52 and vitamin E 54. The bacterial inter-nutrient network control component 24 is lavender floral water 62 extracted only from the flower buds of true lavender by natural cultivation. Lavender floral water 62 is extracted by a low-temperature distillation extraction method. The Th17 cell activation prevention component 26 is persimmon tannin 56.

[0254] For oral cosmetics, water, humectants, etc. are further required. The oral cosmetics according to the present invention include floral water as water, concentrated glycerin, propylene glycol, and butylene glycol as humectants, sodium polyacrylate as a binder, 1-menthol as a cooling agent, peppermint oil as a flavoring agent, herbal oil as a probiotic, fulvic acid 46 collected from humic shale, EM-X Gold 44 as a fermentation active ingredient, plant-derived antibacterial and bactericidal agents, vitamins C 52 and E 54 as antioxidants, lavender floral water extracted only from the flower buds of true lavender by natural cultivation as the bacterial inter-nutrient network control component 24, phytic acid, hinokitiol, lemongrass, aged garlic extract, and mugwort extract as bactericidal and antibacterial agents, vitamins C 52 and E 54 as antioxidants, lavender floral water extracted only from the flower buds of true lavender by natural cultivation as the bacterial inter-nutrient network control component 24, and persimmon tannin 56 as the Th17 cell activation prevention component 26.

[0255] This agent is adjusted to be slightly acidic (pH 6.74) in trace amounts for the purpose of promoting useful resident bacteria, suppressing harmful bacteria, and protecting enamel.

[0256] These components do not have their respective functions independently. For example, lavender floral water extracted only from the flower buds of true lavender by natural cultivation, when combined with other components, is known to have antibacterial, anti-inflammatory, sedative, nerve-strengthening, and analgesic effects. It is necessary to exert the total effect through the interaction by the synergy cycle by combining the effects of each component, and the formulation and effects need to be studied over a long period.

[0257] There are a variety of general conditions in the development of cosmetics, and several important conditions are listed below.

[0258] The product having appropriate fluidity affects the ease of application and use. Especially when developing a texture such as a gel, uniform and smooth fluidity is required. Since cosmetics have a moisturizing effect and exert an auxiliary effect of preventing skin dryness and maintaining a healthy state, the selection of moisturizing components is important. Also, it is necessary to keep the components of the product stable over a long period. By appropriately selecting antioxidants and the like, the effects and texture of the product can be maintained. Regarding stability, by including antioxidants and antioxidant components, the effects are less likely to be impaired even when the components are exposed to air and light, and it becomes possible to extend the life of the product.

[0259] It is also important whether the components used do not mutually have an adverse effect on each other. Examine the compatibility of the components and formulate them so that chemical reactions and separations do not occur. The packaging of cosmetics is also important to maintain the stability and effects of the components. Also, it is necessary to consider an appropriate storage method.

[0260] There are legal regulations in the manufacture and sale of cosmetics. It is necessary to confirm that the product meets the legal standards. It is important to comply with the regulations regarding the list and display method of components, quality control, etc.

[0261] In addition to these conditions, it is important to consider various factors according to the purpose of the product and the target market. While meeting general conditions, it is necessary for the product being implemented to be designed to meet the needs of customers.

[0262] The present invention is an oral cosmetic that satisfies performance by exerting a synergistic effect due to the interaction of each component. It is necessary to pre-test the compatibility of each component to confirm whether there are any adverse effects. Hereinafter, specific test methods will be described.

[0263] (1) Physical mixing test: Mix the components together and observe whether any chemical reactions or interactions occur. If changes such as discoloration, precipitation, or gelation are observed, there may be a problem with the compatibility.

[0264] (2) Thermal stability test: Heat the components together and examine whether there are any interactions or decompositions at high temperatures. If deterioration or abnormal changes are observed, attention should be paid to the compatibility.

[0265] (3) Microscopic observation: Use a microscope to observe the mixture of components and confirm minute changes or interactions. For example, precipitation or aggregation of particles may be observed.

[0266] (4) Test using a fluorescent dye: Add a fluorescent dye and observe how the mixture of components reacts. If a change in fluorescence is observed, there may be a problem with the compatibility.

[0267] (5) In vitro test: Use a test system such as cell culture to examine the effect of the mixture of components on cells. If changes in cell viability or function are observed, there may be a problem with the compatibility.

[0268] (6) Chemical analysis: Use chemical analysis techniques such as HPLC or mass spectrometry to track the reactions and changes of the components. If specific chemical changes are detected, attention should be paid to the compatibility.

[0269] (7) Use of interaction prediction software: It is also possible to predict the interaction of components using in-silico (simulation on a computer) tools. Compatibility is evaluated based on the analysis of chemical structures.

[0270] It is important to combine these test methods to evaluate the interactions and changes when different components are mixed. Also, in order to confirm that the components used as prebiotics are stable and have a good effect on the skin, it is necessary to carefully consider multiple methods. Furthermore, there are the following tests.

[0271] (1) Patch test: Attach a mixture of components to a person's skin for a certain period of time to check for allergic reactions or irritation. If redness or itching of the skin is observed, there may be a problem with the compatibility of the components.

[0272] (2) pH test: Examine the pH value of the components and check whether it matches the pH of the skin. If it is within an appropriate pH range, it can be said that the affinity for the skin is high.

[0273] (3) Visual evaluation: Visually evaluate whether the color, texture, fragrance, etc. of the components harmonize without a sense of incongruity as a whole mixture.

[0274] (4) Stability test: Store a mixture of components for a certain period of time and observe the changes during that period. Check whether separation or discoloration occurs.

[0275] (5) Touch test: Apply a mixture of components to the skin and evaluate the feel. Check whether the texture is easy to get used to on the skin.

[0276] (6) Microbial contamination test: It is possible to conduct a test to examine how resistant the prepared product is to microbial contamination. This can maintain the stability and quality of the product.

[0277] (7) Skin irritation test: Apply the product to the skin of human subjects and evaluate whether there are any irritations or allergic reactions. This can minimize the risk of skin problems.

[0278] (8) Skin permeability test: A test to examine whether the components of the product can pass through the skin and be absorbed into the body. It is particularly important for preventing the absorption of harmful substances.

[0279] (9) Sensitivity test: Have multiple subjects use the product and check whether there are differences in skin sensitivity and reactions. Due to individual differences, evaluate whether it is suitable for a specific person.

[0280] (10) Safety test: Conduct toxicity tests, skin irritation tests, etc. to evaluate the safety of the product during long-term use.

[0281] (11) Field use test: Test the product in an environment close to the actual usage situation to confirm its effectiveness and safety. Consider how users use the product and evaluate its practicality.

[0282] (12) Tolerance test: Confirm the skin tolerance by continuously using the product over a long period. Observe whether there are any allergic reactions or irritations.

[0283] (13) Stability test: A test to confirm whether the product deteriorates or degrades during storage. Consider the effects of temperature changes and light to identify the optimal storage conditions for maintaining the product quality.

[0284] (14) Microbiological test: A test to confirm whether there are any microorganisms growing in the product. Confirm that the product is not contaminated by microorganisms such as bacteria and mold.

[0285] (15) Effect evaluation: Conduct tests to objectively evaluate the effects of the product. For example, consider and implement methods for evaluating moisturizing effects, antioxidant effects, etc.

[0286] Through these tests, it is important to evaluate the compatibility and stability of the components, the effects on the skin, etc., and to ensure the quality and efficacy of the product. It is also advisable to cooperate with experts and cosmetics developers to design and conduct appropriate test protocols. Additionally, it is important to evaluate the component compatibility, efficacy, safety, and tolerance of the product. Design a meticulous test process to ensure the quality and safety of the product.

[0287] Based on the data and results obtained through the tests described above, specific examples of productization are shown below.

Example

[0288] 1. Base Moisture: Aromatic distilled water (1 - 1.5%) Humectant: Concentrated glycerin (6%) Propylene glycol (0.3%) Butylene glycol (1%) 2. Binder: Sodium polyacrylate (2%) 3. Cooling agent: l-menthol (0.02%) 4. Fragrance: Peppermint oil (0.01%) Lemongrass floral water (1%) 5. Bactericidal / antibacterial components, bacterial inter-nutrient network control components, Th17 cell activation prevention components: Phytontide extract (PT-150 Cysxl0: trade name) (1%) Hinokitiol (0.05%) Vitamin C (0.05%) Vitamin E (0.05%) Persimmon tannin (3%) Sodium lactate solution (0.3%) Black garlic extract (Kolic: registered trademark) (1%) Japanese mugwort extract (0.5%) Lavender floral water (18%) Lemongrass (0.01%) 6. Bacterial flora balance regulator Rosemary oil and distilled aromatic water (3%) Full Boric Acid (1%) EM-X Gold (Registered Trademark) (1%)

[0289] Three types of beneficial bacteria (photosynthetic bacteria, lactic acid bacteria, yeast) that are safe in nature are at the center, and the bacteria repeat a lot of "fermentation" to produce bacteria. Yogurt is characterized by taking in lactic acid bacteria into the body, but EM-X GOLD44 maximizes the power of "fermentation", so the bacteria break down the components finely to a size that reaches every corner of the body. Hundreds of gentle components produced by beneficial bacteria support the health of the body.

[0290] Vitamins and minerals play a role in maintaining the health of cells and supporting the regeneration and repair of oral mucosa. Due to the synergistic effect of a combination of various types of natural herbal medicines, it is expected to support the balance of the oral resident flora and the health of the oral mucosa at the same time. Maintaining the balance of the skin resident flora in weakly acidic skin can be expected to have an emollient effect, and it can also be used together as skin care for dryness due to aging and various skin troubles.

[0291] Many of the components used in the oral cosmetics according to the present invention are individually known, but it is not a matter of whether each component exists alone or not, or whether there is an effect simply by combining them. Each component is necessary, and in order to realize a synergistic cycle by optimizing the formulation and exert the total effect, it is extremely important to adjust the pH value to weakly acidic.

[0292] In order to confirm the effectiveness according to this example, a clinical trial was conducted. The evaluation of the results of the clinical trial was carried out by the following method. It was carried out by the Periodontal inflamed surface area (PISA) method. The periodontal inflammation surface area is a clinical index of periodontal disease that represents the area of the inflamed part of the periodontal tissue. Periodontitis is a disease caused by inflammation of the gums and surrounding tissues, and if it progresses, there is a possibility of alveolar bone resorption and destruction of the tissues supporting the teeth.

[0293] PISA (Periodontal Inflamed Surface Area (mm2)) is an indicator that shows the total surface area of the periodontal tissues inflamed by periodontal disease. Periodontal disease is a disease that causes gum inflammation. This indicator shows how extensive the lesion is, and when the spread of inflammation is large, treatment is likely to be necessary. Dental PISA provides important information to dentists for the management and treatment of periodontal disease and helps maintain or improve the patient's oral health to an optimal state.

[0294] The higher the value of PISA, the more advanced the progression of periodontitis, indicating that the damage to the periodontal tissues may be progressing. It is regarded as an indicator useful for the early detection of periodontitis and the evaluation of treatment effects. Since the calculation method and evaluation criteria of PISA have been standardized by dentists and researchers, PISA has become one of the important tools for grasping the degree of progression of periodontitis in the diagnosis and management of periodontitis.

[0295] Periodontal pocket depth (PPD, Pocket Probing Depth): The gap between the gum and the tooth is the periodontal pocket, and as periodontitis progresses, the periodontal pocket may become deeper. PPD refers to the distance from the boundary between the tooth and the gum to the depth of the gum when diagnosing periodontal disease and formulating a treatment plan. The periodontal tissues, together with the teeth, are the tissues that support the teeth, and periodontal disease is a disease that causes inflammation and damage to the gums and other periodontal tissues. PPD helps evaluate the health status of the periodontal tissues and the degree of progression of periodontal disease by measuring the depth from the junction of the tooth and the gum to below the gum. When the pocket between the gum and the tooth becomes deeper, bacteria and dental plaque are likely to accumulate, increasing the likelihood of inflammation and tissue damage progressing.

[0296] Bleeding On Probing (BOP): Probing is to measure the pocket during the periodontal disease examination. A pocket probe is gently inserted (with a force of 15 - 20 g) into the gingival sulcus or pocket, and after being withdrawn (20 - 30 seconds later), the presence or absence of bleeding is examined. In the case of normal gingiva, there is no bleeding or suppuration. When there is inflammation, it bleeds with a light stimulus such as brushing or probing (bleeding tendency). As it progresses further, it bleeds spontaneously (spontaneous bleeding). When the treatment is still insufficient or there is a recurrence, it becomes BOP+.

[0297] Clinical Attachment Level (CAL): The distance from the gingival margin to the bottom of the periodontal pocket is an indicator showing the reduction of the tissue surrounding the gingiva and teeth due to the progression of periodontitis. CAL is the value measured for the depth from the gingival margin to the bottom of the periodontal pocket. By this, it is possible to evaluate how much the connection of the periodontal tissue has been lost from the contact point between the gingiva and the tooth. CAL is useful for evaluating the progression of periodontal disease and is used in combination with the Pocket Probing Depth (PPD) to judge the health status of the periodontal tissue and the severity of periodontal disease.

[0298] In addition, there is also bacteriological evaluation (periodontal pathogenic bacteria by PCR method). It is a molecular biological technique used in the diagnosis and treatment plan formulation of periodontal disease. It is a method of detecting and amplifying the DNA of specific bacteria causing periodontal disease using PCR (Polymerase Chain Reaction) and evaluating their presence and quantity. Since the presence and types of bacteria are involved in the onset and progression of periodontal disease, bacteriological evaluation provides important information for the evaluation and management of periodontal disease.

[0299] This method is implemented in the following way. First, a sample containing bacteria in the gingiva and periodontal pockets (dental plaque and gingival tissue in the periodontal pockets) is collected from a periodontal disease patient, and bacterial DNA is extracted from the collected sample. This DNA contains the genetic information of the bacteria. Next, a PCR reaction is carried out using the extracted DNA. PCR is a technique for amplifying a specific DNA fragment, and a specific gene fragment of the bacteria is amplified and detected. Then, the amplified DNA fragment is analyzed to quantitatively evaluate the presence and amount of specific periodontal pathogenic bacteria.

[0300] Bacteriological evaluation is used to evaluate whether specific periodontal pathogenic bacteria are present and their amount, which helps to evaluate the progress and risk of the patient's periodontal disease and to formulate an appropriate treatment plan. Since periodontal pathogenic bacteria can affect the progression of the disease state and the treatment effect, bacteriological evaluation becomes an important tool when considering individual treatment strategies.

[0301] To evaluate the effect of the oral cosmetic according to the present invention, this PISA method was incorporated. Here, as a simple evaluation, PPD was used to examine the change in the PPD value before and after the use of the oral cosmetic of the present invention. Also, bacteriological evaluation by the PCR method was performed.

[0302] Figure 5 shows the results of evaluating the effect of the oral cosmetic according to the present invention. The number of clinical cases is 50, and the usage period is 88 days on average. The usage frequency was basically daily, but there were also cases where it could not be used sometimes or could not be used at all, and these were also included.

[0303] The PPD was improved from an average of 3.53 mm before use to an average of 3.17 mm after use. The maximum change in PPD was 0.77 mm. There were 3 cases where the PPD was not improved and there was no effect, but it was due to temporary gum improvement and discontinuation of use. The overall improvement rate was 96%, and the effect was confirmed. PCR analyzed the amplified DNA fragments, observed the presence and amount of specific periodontal pathogenic bacteria, and evaluated them in three levels: good, normal, and poor. There were 5 cases of poor, which means that the presence and amount of periodontal pathogenic bacteria did not change or increased slightly compared to before use. The improvement rate in the PCR evaluation result was also 90%, and the effect was confirmed.

[0304] The product requires user feedback and improvement. It is important to collect feedback from users who actually use the product to understand the effect and usability of the product. This product is intended to contribute to people's health and beauty, and various opinions from users are being feedback.

[0305] Examples of opinions from users are shown below. ● Applied before going to bed and when waking up in the morning, the bad breath was no longer a concern. ● The stickiness in the mouth disappeared. ● There was sometimes bleeding when brushing teeth, but since starting to use the gel, the bleeding has stopped. ● When applied to stomatitis, the pain disappeared and it got better. ● The teeth that were shaky and difficult to brush due to chattering (gum retraction) seem to have stopped shaking. ● When applied to insect bites, the itching subsided. ● The itching of the skin disappeared. ● Due to atopy, I couldn't use cosmetics and was troubled, so this was helpful.

[0306] By comprehensively evaluating these results, the effectiveness of the oral cosmetics according to the present invention became clear.

[0307] As described above, the embodiments of the present invention have been explained, but the present invention includes appropriate modifications that do not impair its purpose and advantages, and furthermore, is not limited by the above embodiments.

Explanation of Symbols

[0308] 10 Bacterial Flora Balance Adjustment A 12 Prebiotic Agent 14 Plant Minerals 16 EM Agent 18 Vitamin Agent 20 Bactericidal and Antibacterial Components 22 Antioxidant Components 24 Bacterial Inter-Nutritional Network Control Component Bacteria 26 Th17 Cell Activation Prevention Component 30 Bacterial Flora Balance Adjustment B 40 Synergy Cycle 42 Rosemary Oil and Distilled Aromatic Water 44 EM-X Gold 46 Fulvic Acid 48 Phytontid 50 Hinokitiol 52 Vitamin C 54 Vitamin E 56 Persimmon Tannin 58 Aged Garlic Extract 60 Mugwort Extract 62 Lavender Free Floral Water 64 Lemongrass 70 Evaluation Results

Claims

1. A prebiotics agent made of natural materials that promotes the growth of beneficial bacteria, vegetable minerals, vitamin agents, EM (Effective Microorganisms) agents, are composed of components, By the synergistic effect of the prebiotics agent, the vegetable minerals, the vitamin agents, and the EM preparation, maintaining the balance of the oral resident flora and the balance of the skin resident flora in weakly acidic skin, An oral cosmetic characterized by this.

2. Further containing fermented garlic, The oral cosmetic according to Claim 1, characterized by this.

3. Further containing a component for controlling the nutritional network between bacteria, The oral cosmetic according to Claim 1, characterized by this.

4. Further containing a component for preventing Th17 cell activation, The oral cosmetic according to Claim 1, characterized by this.

5. Further containing an antibacterial and preservative agent derived from plants The oral cosmetic according to Claim 1, characterized by this.

6. The prebiotics agent made of natural materials is a herb, The oral cosmetic according to Claim 1, characterized by this.

7. The herb is chamomile, lavender, lemongrass, sage herb, or rosemary, The oral cosmetic according to Claim 6, characterized by this.

8. The chamomile is extracted and used as chamomile oil from the flowers of the chamomile, The oral cosmetic according to Claim 6, characterized by this.

9. The lavender is used by extracting the flowers of the lavender as lavender oil. The oral cosmetic according to claim 6, characterized in that.

10. The lavender oil is extracted only from the flower buds of true lavender by natural cultivation. The oral cosmetic according to claim 9, characterized in that.

11. The lemongrass is used by extracting lemongrass essential oil from at least one of the leaves and stems of the lemongrass. The oral cosmetic according to claim 6, characterized in that.

12. The sage herb is used by extracting sage herb essential oil from at least one of the leaves and flowers of the sage herb. The oral cosmetic according to claim 6, characterized in that.

13. The rosemary is used by extracting rosemary essential oil from at least one of the leaves and flowers of the rosemary. The oral cosmetic according to claim 6, characterized in that.

14. The chamomile oil, the lavender oil, the lemongrass essential oil, the sage herb essential oil, and the rosemary essential oil are produced and extracted by a low-temperature distillation extraction method that does not destroy the components. The oral cosmetic according to claims 8 to 13, characterized in that.

15. The plant-based mineral is fulvic acid collected from humic shale. The oral cosmetic according to claim 1, characterized in that.

16. With partial amendment The antibacterial and preservative agent derived from plants is phytic acid, hinokitiol, aged garlic extract or mugwort extract. The oral cosmetic according to claim 1, characterized in that.

17. The vitamin agent is vitamin C or vitamin E. The oral cosmetic according to claim 1, characterized in that.

18. The bacterial inter-nutritional network control component is lavender floral water extracted only from the flower buds of organically cultivated true lavender. The oral cosmetic according to claim 2, characterized in that.

19. The lavender floral water is extracted by a low-temperature distillation extraction method. The oral cosmetic according to claim 9, characterized in that.

20. The Th17 cell activation prevention component is persimmon tannin. The oral cosmetic according to claim 3, characterized in that.

21. Purified water as the moisture, Concentrated glycerin, propylene glycol, butylene glycol as the humectant Sodium polyacrylate as the binder, 1-menthol as the cooling agent, Peppermint oil as the flavoring agent, Herb oil as the probiotics, Fulvic acid collected from humic shale, EM bacteria or fermented garlic as the fermentation active ingredient, As the antibacterial and bactericidal agent derived from plants, phytic acid, hinokitiol, lemongrass, aged garlic extract or mugwort extract, Vitamin E or vitamin C as the antioxidant, Lavender floral water extracted only from the flower buds of true lavender grown by natural cultivation as a bacterial trophic network control component, and Lavender floral water extracted only from the flower buds of true lavender grown by natural cultivation as a bacterial trophic network control component, and Persimmon tannin, which is the Th17 cell activation prevention component, and An oral cosmetic composed of the above components.

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