Ejection device

Abstract

To provide an ejection device that can gradually eject two kinds of liquid in an interlocking manner.SOLUTION: An ejection device 10 has: a first syringe 30 which includes a first pusher 34; a second syringe 50 which includes a second pusher 54; a first path 66 which communicates with a nozzle head of the first syringe 30; a first ejection part 68 which communicates with the first path 66 and ejects liquid; a second path 67 which communicates with a nozzle head of the second syringe 50; and a second ejection part 69 which communicates with the second path 67 and ejects liquid, wherein the device also has an interlocking mechanism 90 which interlocks the movement of the first pusher 34 with the movement of the second pusher 54, a first pressing part 40 pressed by the first pusher 34 can move in a pressing direction from a first position to a third position via a second position, and the interlocking mechanism 90 allows the first syringe 30 to eject liquid when the first pressing part 40 moves from the first position to the second position, and allows the second syringe 50 to eject liquid when the first pressing part 40 moves from the second position to the third position.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a discharge device that discharges two types of liquid. [Background technology]

[0002] In recent years, a treatment has been performed in which cultured cells are formed into a sheet-like culture and then transplanted onto the surface of an injured organ. When the sheet-like cell culture is transplanted onto the surface of an organ, it can survive for a long time, using the blood vessels on the surface of the organ as a vascular bed.

[0003] Sheet-shaped cell cultures, which are aggregates of multiple cultured cells, are fragile and prone to deformation and breakage, such as curling. For this reason, sheet-shaped cell cultures are subjected to a reinforcement treatment to maintain their aggregate shape. One example of the reinforcement treatment is a method in which fibrinogen and thrombin solutions, which gel when mixed, are sprayed onto the sheet-shaped cell culture. For example, fibrinogen solution is dripped evenly onto the sheet-shaped cell culture, and then thrombin solution is sprayed onto the sheet-shaped cell culture, causing the two liquids to mix and gel in the sheet-shaped cell culture. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Laid-Open No. 2006-95098 Summary of the Invention [Problem to be solved by the invention]

[0005] However, the above-mentioned method requires switching between a device for discharging the fibrinogen solution and a device for discharging the thrombin solution, which makes the process complicated and time-consuming, and may result in uneven processing due to manual labor. To prevent uneven processing due to manual labor, a device capable of discharging a mixture of two types of liquids, such as that shown in Patent Document 1, may be used. However, in the device described in Patent Document 1, the plungers of two syringes containing different liquids move together, so the two liquids are discharged simultaneously. Therefore, this device cannot discharge the two liquids at different times.

[0006] The present invention has been made to solve the above-mentioned problems, and has an object to provide a discharge device that can discharge two types of liquid in stages in a linked manner. [Means for solving the problem]

[0007] The above object can be achieved by the invention described in (1) below.

[0008] (1) A discharge device according to the present invention includes a first syringe having a first plunger, a second syringe having a second plunger, a first path communicating with a first tip of the first syringe, a first discharge part communicating with the first path to discharge a liquid, a second path communicating with a second tip of the second syringe, and a second discharge part communicating with the second path to discharge a liquid, the discharge device further including a linkage mechanism that links the movement of the second plunger in the pressing direction with the movement of the first plunger in the pressing direction, and the pressing part that is pressed by the first plunger is movable in the pressing direction from a first position to a third position via a second position, and the linkage mechanism causes the liquid to be discharged from the first syringe when the pressing part moves from the first position to the second position, and causes the liquid to be discharged from the second syringe, or from both the first syringe and the second syringe, when the pressing part moves from the second position to the third position. [Effects of the Invention]

[0009] The discharge device described in (1) above has a linkage mechanism that links the movement of the second plunger with the movement of the first plunger, so that by simply pressing the pressing part of the first syringe, liquid can be discharged from the first syringe and then from the second syringe, or from the first syringe and the second syringe. Therefore, the discharge device can discharge two types of liquid in stages in a linked manner.

[0010] (2) In the ejection device described in (1) above, the first pusher may have a base-side pusher and a tip-side pusher that can move more distally than the base-side pusher, the base-side pusher and the tip-side pusher have slidable contact surfaces and can move together due to the frictional force of the contact surfaces, the base-side pusher has a first engaging portion, and the second pusher has a second engaging portion that can come into contact with and engage with the first engaging portion that moves distally, and the interlocking mechanism may be formed by the base-side pusher having the first engaging portion, the tip-side pusher, and the second pusher having the second engaging portion. As a result, the ejection device ejects liquid from the first syringe using a first pusher having a base end pusher and a tip end pusher that can move together due to the frictional force of the contact surfaces, and then slides the contact surfaces of the base end pusher and tip end pusher to contract the first pusher, while engaging the first engagement portion with the second engagement portion to move the second pusher, thereby ejecting liquid from the second syringe.

[0011] (3) In the discharge device described in (1) or (2) above, a discharge implement having the first path, the first discharge portion, the second path, and the second discharge portion may be connectable and detachable to a syringe having the first syringe and the second syringe. This allows the discharge implement to be connected to the syringe after filling the first syringe and the second syringe with liquid, thereby improving workability.

[0012] (4) The discharge device according to any one of (1) to (3) above may have a first filling auxiliary part including a closing part that closes the second tip of the second syringe and a first filling tip that communicates with the first tip of the first syringe. This allows the discharge device to easily fill the first syringe with a fluid by using the first filling auxiliary part.

[0013] (5) The discharge device according to any one of (1) to (4) above may further include a second filling auxiliary part including a closing part that closes the first nozzle of the first syringe and a second filling nozzle that communicates with the second nozzle of the second syringe. This allows the discharge device to easily fill the second syringe with a fluid by using the second filling auxiliary part.

[0014] (6) In the discharge device according to any one of (1) to (5) above, the second syringe may be disposed concentrically inside the first syringe, thereby preventing the second syringe, which is linked to the operation of the first syringe, from being mistakenly operated.

[0015] (7) In the discharge device according to any one of (1) to (6) above, the second syringe may be disposed outside the first syringe, side by side. This makes it easy to operate each of the first syringe and the second syringe, thereby improving operability of the discharge device.

[0016] (8) In the discharge device according to any one of (1) to (7) above, the hole of the second discharge part may be smaller than the hole of the first discharge part, which makes it easier for the discharge device to drip the liquid from the first discharge part and to spray the liquid from the second discharge part.

[0017] (9) In the discharge device described in (8) above, the number of holes in the second discharge section may be greater than the number of holes in the first discharge section. Because the holes in each second discharge section are small and can only discharge a small amount of liquid, increasing the number of holes in the second discharge section can appropriately ensure the total amount of liquid discharged from the multiple second discharge sections. [Brief explanation of the drawings]

[0018] [Figure 1] FIG. 1 is a cross-sectional view showing a discharge device according to an embodiment. [Figure 2] FIG. 2 is a perspective view showing a syringe and a dispensing tool. [Figure 3] FIG. 4 is a plan view showing the lower surface of the lid portion on which the discharge portion is formed. [Figure 4] 10A and 10B are cross-sectional views illustrating a method for filling syringes with a first liquid and a second liquid using an ejection device, in which (A) shows the state in which the second filling auxiliary part is connected to the second syringe, (B) shows the state in which the second syringe has been filled with the second liquid, and (C) shows the state in which the first syringe has been filled with the first liquid. [Figure 5] 1A and 1B are cross-sectional views illustrating a method for ejecting the first and second liquids onto a sheet-shaped cell culture using an ejection device, in which (A) shows the state in which the syringe is connected to the ejection tool, (B) shows the state in which the first liquid has been ejected from the first syringe, and (C) shows the state in which the second liquid has been ejected from the second syringe. [Figure 6] FIG. 10 is a cross-sectional view showing a modified example of the discharge device. DETAILED DESCRIPTION OF THE INVENTION

[0019] Hereinafter, an embodiment of the present invention will be described with reference to the drawings. Note that dimensions in the drawings may be exaggerated for convenience of explanation and may differ from actual dimensions. Furthermore, in this specification and the drawings, components having substantially the same functions are designated by the same reference numerals, and redundant description will be omitted. In this specification, the nozzle side of the syringe 20 will be referred to as the "tip side," and the plunger side will be referred to as the "base side." Furthermore, the direction in which the plunger is pressed to eject liquid from the syringe 20 will be referred to as the "pressing direction."

[0020] As shown in Figures 1 and 2, the ejection device 10 of this embodiment is a device that ejects two types of liquids that gel when mixed in a coordinated manner in stages to perform a reinforcement process on a sheet-shaped cell culture 100, which is a sheet-shaped aggregate of multiple cells.

[0021] The discharge device 10 includes a syringe 20, a discharge implement 60, a first filling auxiliary part 70, and a second filling auxiliary part 80.

[0022] The syringe 20 has a first syringe 30 capable of containing a first liquid, and a second syringe 50 disposed inside the first syringe 30 and capable of containing a second liquid.

[0023] The first syringe 30 has a first outer cylinder 31 capable of containing a first liquid, a first cylindrical tip 32 communicating with the first outer cylinder 31, a first gasket 33 slidably fitted to the inner surface of the first outer cylinder 31, and a first plunger 34 that applies force to the first gasket 33 from outside the first syringe 30.

[0024] The second syringe 50 has a second outer cylinder 51 capable of containing a second liquid, a second cylindrical tip 52 which is an inlet and outlet for the liquid to enter and exit the second outer cylinder 51, a second gasket 53 which is slidably fitted to the inner surface of the second outer cylinder 51, and a second plunger 54 which applies force to the second gasket 53 from outside the second syringe 50.

[0025] The first outer cylinder 31 is disposed outside the second outer cylinder 51 and concentrically with the second outer cylinder 51. The first outer cylinder 31 forms a space between itself and the second outer cylinder 51 on the inside for accommodating the first liquid.

[0026] The first cylindrical tip 32 is an inlet / outlet for liquid to the first outer cylinder 31, and is connectable to a discharge tool 60. The first cylindrical tip 32 is formed with a first connecting part 35 that is connectable to the discharge tool 60. The first connecting part 35 has, for example, a screw structure.

[0027] The first gasket 33 is a member that tightly adheres to the inner surface of the first outer tube 31 and the outer surface of the second outer tube 51 and slides against the inner surface of the first outer tube 31 and the outer surface of the second outer tube 51 in the stroke direction of the first plunger 34.

[0028] The first pusher 34 includes a base-side pusher 36 having a first pressing portion 40 that is pressed by the user, and a tip-side pusher 37 that is movable distally relative to the base-side pusher 36 and is connected to the first gasket 33. The base-side pusher 36 has a first stopper 38 that protrudes inward from the inside of its distal end, and the tip-side pusher 37 has a second stopper 39 that protrudes outward from the outside of its base-side end. The inner circumferential surface of the base-side pusher 36 tightly adheres to the outer circumferential surface of the tip-side pusher 37. Therefore, the base-side pusher 36 and the tip-side pusher 37 move together without sliding against each other unless they receive a force that exceeds the frictional force of their contact surfaces. Note that the base-side pusher 36 and the tip-side pusher 37 can slide against each other if they receive a force that exceeds the frictional force of their contact surfaces. Note that, although the base-side pusher 36 is disposed outside the tip-side pusher 37 in this embodiment, it may also be disposed inside. In this case, the first stopper 38 is disposed on the outside of the tip end of the base-side pusher 36, and the second stopper 39 is disposed on the inside of the base-side end of the tip-side pusher 37. In addition, the base-side pusher 36 has a first engagement portion 41 on the first pressing portion 40 that can engage with the second pusher 54.

[0029] The second outer cylinder 51 is disposed inside the first outer cylinder 31 and concentrically with the first outer cylinder 31. The second outer cylinder 51 forms a space therein for accommodating the second liquid. The second outer cylinder 51 has a movement restricting portion 59 protruding inward from the inside of the end portion on the base end side to restrict movement of the second gasket 53 (or the second plunger 54) toward the base end. The second outer cylinder 51 is fixed to the first outer cylinder 31 by at least one first fixing portion 58 outside the movement range of the first gasket 33 (for example, between the second cylinder tip 52 and a portion of the first gasket 33 with a constant outer diameter on which the first gasket 33 slides) so as not to interfere with the movement of the first gasket 33. Each first fixing portion 58 is formed in a portion of the circumferential direction so as not to interfere with the flow of the first liquid.

[0030] The second cylindrical tip 52 is an inlet / outlet for liquid to the second outer cylinder 51, and is connectable to a discharge tool 60. The second cylindrical tip 52 is formed with a second connecting part 55 that is connectable to the discharge tool 60. The second connecting part 55 has, for example, a screw structure.

[0031] The second gasket 53 is a member that tightly contacts the inner peripheral surface of the second outer cylinder 51 and slides against the inner peripheral surface of the second outer cylinder 51 in the stroke direction of the second plunger .

[0032] The tip end of the second pusher 54 is connected to the second gasket 53. The second pusher 54 has a second pressing portion 56 at its base end that is pressed by the first pressing portion 40. The second pressing portion 56 has a second engaging portion 57 that can engage with the first engaging portion 41 of the proximal pusher 36. The surface of the second engaging portion 57 facing the base end is located distal to the surface of the first engaging portion 41 facing the distal end, and can come into contact with the surface of the first engaging portion 41 facing the distal end. Therefore, the second engaging portion 57 can move distally beyond the first engaging portion 41, but cannot move proximally beyond the first engaging portion 41. Before use, the first engaging portion 41 and the second engaging portion 57 are bonded together with a removable adhesive.

[0033] The discharge tool 60 has a culture dish 61 (culture substrate), a storage section 62 that stores the culture dish 61, and a lid section 63 that fits over the storage section 62 to cover the culture dish 61. The lid section 63 has a first discharge side connector 64 to which the first connector 35 of the first nozzle 32 can be connected, a second discharge side connector 65 to which the second connector 55 of the second nozzle 52 can be connected, a first path 66 that communicates with the first discharge side connector 64, a second path 67 that communicates with the second discharge side connector 65, a plurality of first discharge sections 68 that communicate with the first path 66, and a plurality of second discharge sections 69 that communicate with the second path 67.

[0034] The first discharge side connecting portion 64 is formed with a thread structure that allows connection to the first connecting portion 35 of the first cylindrical tip 32, and the second discharge side connecting portion 65 is formed with a thread structure that allows connection to the second connecting portion 55 of the second cylindrical tip 52. The first discharge side connecting portion 64 and the second discharge side connecting portion 65 are formed on the upper surface of the lid portion 63. The second discharge side connecting portion 65 is disposed inside the first discharge side connecting portion 64 and concentrically with the first discharge side connecting portion 64. Therefore, the first connecting portion 35 and the second connecting portion 55 can be connected to the first discharge side connecting portion 64 and the second discharge side connecting portion 65 at the same time.

[0035] As shown in Figures 1 and 3, the multiple first discharge parts 68 are formed on the underside of the lid part 63, on a surface located above the culture dish 61. The multiple first discharge parts 68 are arranged approximately uniformly above the culture dish 61. The holes of each first discharge part 68 are formed to be somewhat large so that the liquid can drip, but they may also be sprayed. The first path 66 is preferably formed so as to branch so as to connect the first discharge side connecting part 64 and the multiple first discharge parts 68.

[0036] The plurality of second discharge portions 69 are formed on the underside of the lid portion 63, above the culture dish 61. The plurality of second discharge portions 69 are arranged substantially uniformly above the culture dish 61. The holes of each second discharge portion 69 spray the liquid, but may also drip the liquid. The second path 67 is preferably formed by branching so as to connect the second discharge side connection portion 65 and the plurality of second discharge portions 69. The size of the holes of the second discharge portions 69 is smaller than the size of the holes of the first discharge portions 68. This makes it easy for the first liquid to drip from the first discharge portions 68 and the second liquid to spray from the second discharge portions 69. The number of second discharge portions 69 is greater than the number of first discharge portions 68. Each second discharge portion 69 is small and can only discharge a small amount, but by increasing the number of second discharge portions 69, the amount of second liquid discharged from the second discharge portions 69 can be appropriately ensured.

[0037] The first filling auxiliary part 70 is a member that assists in filling the first syringe 30 with the first liquid. As shown in FIG. 1 , the first filling auxiliary part 70 has a first filling connection part 71 having a thread structure to which the first connection part 35 of the first cylindrical tip 32 can be connected, a second closing connection part 72 (closing part) having a thread structure to which the second connection part 55 of the second cylindrical tip 52 can be connected, a first filling tip 73 that communicates with the first filling connection part 71, and at least one second fixing part 74 that fixes the first filling connection part 71 and the second closing connection part 72. The first connection part 35 and the second connection part 55 can be simultaneously connected to the first filling connection part 71 and the second closing connection part 72. The second closing connection part 72 is open on the side where the second connection part 55 is inserted and closed on the opposite side. When the first filling auxiliary part 70 is connected to the syringe 20, the second cylindrical tip 52 of the second syringe 50 is closed by the second closing connection part 72, and liquid can be filled into the first syringe 30 from the first filling cylindrical tip 73. Each second fixing part 74 is formed in a part of the circumferential direction at a position different from the thread structure so as not to hinder the movement of the first cylindrical tip 32 or the second cylindrical tip 52 and not to hinder the flow of the first liquid.

[0038] The second filling auxiliary part 80 is a member that assists in filling the second syringe 50 with the second liquid. The second filling auxiliary part 80 has a first closing connection part 81 (closing part) to which the first connection part 35 of the first cylindrical tip 32 can be connected, a second filling connection part 82 to which the second connection part 55 of the second cylindrical tip 52 can be connected, and a second filling cylindrical tip 83 that communicates with the second filling connection part 82. The first connection part 35 and the second connection part 55 can be simultaneously connected to the first closing connection part 81 and the second filling connection part 82. The first closing connection part 81 is open on the side where the first connection part 35 is inserted and is closed on the opposite side. When the second filling auxiliary part 80 is connected to the syringe 20, the first nozzle 32 of the first syringe 30 is closed by the first closing connection part 81, making it possible to fill liquid into the second syringe 50 from the second filling nozzle 83.

[0039] The first liquid filled in the first syringe 30 and the second liquid filled in the second syringe 50 contain substances that gel, increase in viscosity, or harden when mixed. In this embodiment, the first liquid is a fibrinogen liquid, and the second liquid is a thrombin liquid. When the fibrinogen liquid and the thrombin liquid are mixed, the fibrinogen becomes fibrin due to the action of thrombin, and gels. Note that gelation also increases viscosity and hardness, so it also increases viscosity or hardening. Alternatively, the substances that gel when mixed are not particularly limited and may be, for example, a first aqueous solution of NHS-CM dextrin and trehalose hydrate and a second aqueous solution containing sodium carbonate and sodium bicarbonate.

[0040] The cells to be cultured include, for example, differentiated cells derived from somatic stem cells, adult stem cells, mesenchymal stem cells, ES cells (embryonic stem cells), or iPS cells (induced pluripotent stem cells). Somatic stem cells include, for example, skeletal myoblasts (myoblast cells). Differentiated cells include, for example, cardiomyocytes and fibroblast cells.

[0041] Next, a discharge method using the discharge device 10 according to this embodiment will be described.

[0042] First, as shown in Fig. 4(A), the user prepares syringe 20 with first gasket 33, first plunger 34, second gasket 53, and second plunger 54 arranged at the most distal end. Next, the user connects first closing connection part 81 and second filling connection part 82 of second filling assistant part 80 to first connection part 35 and second connection part 55 of syringe 20. This closes first nozzle 32 of first syringe 30.

[0043] Next, the user inserts the second filling nozzle 83 of the second filling assisting part 80 into a container containing thrombin liquid (second liquid) and pulls the first pressing part 40 of the first pusher 34 toward the proximal end, as shown in FIG. 4(B). At this time, because the first nozzle 32 is closed by the second filling assisting part 80, the first gasket 33 hardly moves, and the movement of the distal pusher 37 is restricted. Therefore, the proximal pusher 36 and the distal pusher 37 of the first pusher 34 slide relative to each other due to a force exceeding the frictional force of their contact surfaces. In other words, the distal pusher 37 does not move, and only the proximal pusher 36 moves toward the proximal end, thereby extending the first pusher 34. The second pressing part 56 of the second pusher 54 is bonded to the first pressing part 40 of the proximal pusher 36. Therefore, as the first pressing portion 40 moves, the second pusher 54 having the second pressing portion 56 and the second gasket 53 move toward the proximal end. As a result, the thrombin solution is sucked from the second filling nozzle 83 and housed in the second outer cylinder 51. When the first stopper 38 of the proximal pusher 36 comes into contact with the second stopper 39 of the distal pusher 37, the proximal pusher 36 and the distal pusher 37 become unable to slide, and further extension of the first pusher 34 is restricted. When the second gasket 53 (or the second pusher 54) comes into contact with the movement restricting portion 59 of the second outer cylinder 51, further movement of the second gasket 53 and the second pusher 54 toward the proximal end is restricted.

[0044] Next, the user removes the second filling aid part 80 from the syringe 20 and connects the first filling aid part 70 as shown in FIG. 4(C). That is, the user connects the first filling connection part 71 and the second closing connection part 72 of the first filling aid part 70 to the first connection part 35 and the second connection part 55 of the syringe 20. This closes the second nozzle 52 of the second syringe 50. Next, the user inserts the first filling nozzle 73 of the first filling aid part 70 into a container containing fibrinogen liquid (first liquid) and pulls the first pressing part 40 of the first plunger 34 toward the proximal end. At this time, the first plunger 34 cannot extend any further due to contact between the first stopper 38 and the second stopper 39, so the first plunger 34 and the first gasket 33 move toward the proximal end. As the first pusher 34 moves toward the proximal end, the second pusher 54, which is adhered to the first pusher 34, receives a force from the first pusher 34 in the proximal end direction, but its movement toward the proximal end is restricted by the movement restricting portion 59. Therefore, the adhesion between the first pusher 34 and the second pusher 54 is separated, and the second pusher 54 does not move, and only the first pusher 34 moves toward the proximal end. As a result, the fibrinogen liquid is sucked from the first filling nozzle 73 and contained in the first outer cylinder 31. The first engaging portion 41 moves toward the proximal end further than the second engaging portion 57.

[0045] Next, as shown in FIG. 5(A), the user removes the first filling auxiliary part 70 from the syringe 20 and connects the discharge tool 60. That is, the user connects the first discharge-side connecting part 64 and the second discharge-side connecting part 65 of the discharge tool 60 to the first connecting part 35 and the second connecting part 55 of the syringe 20. The first pressing part 40 is in the first position. Next, the user presses the first pressing part 40 toward the distal end. At this time, the second engaging part 57 of the second pusher 54 is separated from the first engaging part 41 of the first pusher 34, so the second pusher 54 receives no force and does not move. The proximal-end pusher 36 and the distal-end pusher 37 of the first pusher 34 move together toward the proximal end while remaining extended, as shown in FIG. 5(B). The fibrinogen liquid contained in the first outer cylinder 31 is pressed by the first gasket 33 and discharged from the first outer cylinder 31 through the first path 66 of the discharge tool 60 and from the plurality of first discharge parts 68. The plurality of first discharge parts 68 dispense the fibrinogen liquid in drops, but it may also be sprayed.

[0046] When the first gasket 33 moves to and hits the tip end of the first outer cylinder 31, the movement of the first gasket 33 is restricted. The first engagement portion 41 of the first pusher 34 engages with the second engagement portion 57 of the second pusher 54. At this time, the first pressing portion 40 is in the second position. In this state, the user further presses the first pressing portion 40 toward the tip end. The first pressing portion 40 moves to the third position. As a result, the base-side pusher 36 and the tip-side pusher 37 of the first pusher 34 receive a force exceeding the frictional force of their contact surfaces and slide relative to each other, as shown in FIG. 5(C). In other words, the tip-side pusher 37 does not move, and only the base-side pusher 36 moves toward the tip end, causing the first pusher 34 to contract. When the proximal-end pusher 36 moves toward the distal end, the first engagement portion 41 of the first pusher 34 presses the second engagement portion 57 of the second pusher 54, and the second pusher 54 and the second gasket 53 move toward the proximal end. As a result, the thrombin solution contained in the second outer cylinder 51 is pressed by the second gasket 53 and discharged from the second outer cylinder 51 through the second path 67 of the discharge tool 60 through the multiple second discharge portions 69. The multiple second discharge portions 69 spray the thrombin solution, but it may also drip. This allows the discharge device 10 to discharge the fibrinogen solution and the thrombin solution in a synchronized, stepwise manner onto the sheet-shaped cell culture 100 on the culture dish 61.

[0047] As described above, the discharge device 10 according to this embodiment includes the first syringe 30 including the first plunger 34, the second syringe 50 including the second plunger 54, the first path 66 communicating with the cylindrical tip of the first syringe 30, the first discharge part 68 communicating with the first path 66 to discharge liquid, the second path 67 communicating with the cylindrical tip of the second syringe 50, and the second discharge part 69 communicating with the second path 67 to discharge liquid, and the discharge device 10 includes the first syringe 30 including the first plunger 34, and the second syringe 50 including the second plunger 54. The first pressing portion 40, which is pressed by the first plunger 34, is movable in the pressing direction from a first position (see FIG. 5(A)) via a second position (see FIG. 5(B)) to a third position (see FIG. 5(C)). The interlocking mechanism 90 ejects liquid from the first syringe 30 when the first pressing portion 40 moves from the first position to the second position, and ejects liquid from the second syringe 50, or from the first syringe 30 and the second syringe 50, when the first pressing portion 40 moves from the second position to the third position. As a result, the discharge device 10 has an interlocking mechanism 90 that interlocks the movement of the second plunger 54 with the movement of the first plunger 34, so that by simply pressing the first pressing portion 40 of the first syringe 30, liquid can be discharged from the first syringe 30 and then from the second syringe 50, or from the first syringe 30 and the second syringe 50. Therefore, the discharge device 10 can interlock and discharge two types of liquid in stages with a single operation.

[0048] The first pusher 34 has a base end pusher 36 and a tip end pusher 37 that can move more distally than the base end pusher 36, and the base end pusher 36 and the tip end pusher 37 have slidable contact surfaces and can move together due to the frictional force of the contact surfaces, the base end pusher 36 has a first engagement portion 41, and the second pusher 54 has a second engagement portion 57 that can come into contact with and engage with the first engagement portion 41 that moves distally, and the interlocking mechanism 90 is formed by the base end pusher 36 having the first engagement portion 41, the tip end pusher 37, and the second pusher 54 having the second engagement portion 57. As a result, the ejection device 10 ejects liquid from the first syringe 30 using the first pusher 34, which has a base end pusher 36 and a tip end pusher 37 that can move together due to the frictional force of their contact surfaces, and then slides the contact surfaces of the base end pusher 36 and the tip end pusher 37 to contract the first pusher 34, while engaging the first engagement portion 41 with the second engagement portion 57 to move the second pusher 54, thereby ejecting liquid from the second syringe 50.

[0049] The discharge implement 60, which has the first path 66, the first discharge portion 68, the second path 67, and the second discharge portion 69, and the syringe 20, which has the first syringe 30 and the second syringe 50, can be connected and disconnected. This allows the discharge device 10 to connect the discharge implement 60 to the syringe 20 after filling the first syringe 30 and the second syringe 50 with liquid, thereby improving operability.

[0050] The discharge device 10 has a first filling auxiliary part 70 including a second closing connection part 72 (closing part) that closes the second cylindrical tip 52 of the second syringe 50, and a first filling cylindrical tip 73 that communicates with the first cylindrical tip 32 of the first syringe 30. This allows the discharge device 10 to easily fill the first syringe 30 with a fluid by using the first filling auxiliary part 70.

[0051] The discharge device 10 has a second filling auxiliary part 80 that includes a first closing connection part 81 (closing part) that closes the first cylindrical tip 32 of the first syringe 30, and a second filling cylindrical tip 83 that communicates with the second cylindrical tip 52 of the second syringe 50. This allows the discharge device 10 to easily fill the second syringe 50 with a fluid by using the second filling auxiliary part 80.

[0052] The second syringe 50 is arranged concentrically inside the first syringe 30. This makes it possible for the discharge device 10 to prevent the second syringe 50, which is linked to the operation of the first syringe 30, from being operated by mistake.

[0053] The hole of the second outlet portion 69 is smaller than the hole of the first outlet portion 68. This makes it easier for the discharge device 10 to drip the liquid from the first outlet portion 68 and to spray the liquid from the second outlet portion 69.

[0054] The number of holes in the second discharge portions 69 is greater than the number of holes in the first discharge portions 68. The holes in each second discharge portion 69 are small and can only discharge a small amount, but by increasing the number of holes in the second discharge portions 69, the total amount of liquid discharged from the multiple second discharge portions 69 can be appropriately ensured.

[0055] The present invention is not limited to the above-described embodiment, and various modifications can be made by those skilled in the art within the technical spirit of the present invention. For example, the first syringe 30 does not have to have an extendable structure (a structure having a distal plunger 37 and a proximal plunger 36). Even in this case, by the second engagement portion 57 coming into contact with the first engagement portion 41 while the first plunger 34 is moving, liquid is ejected only from the first syringe 30 before the contact, and liquid is ejected from both the first syringe 30 and the second syringe 50 after the contact.

[0056] 6, the second syringe 50 may be arranged next to the outside of the first syringe 30. This makes it easier to operate each of the first syringe 30 and the second syringe 50 in the discharge device 10, thereby improving operability.

[0057] Furthermore, the object to which the two types of liquids are supplied by the discharge device 10 is not limited to the sheet-shaped cell culture 100. [Explanation of symbols]

[0058] 10. Discharge Device 20 syringes 30 First Syringe 32 No. 1 barrel tip 34 First pusher 36 Base end pusher 37 Tip pusher 40 First pressing part 41 first engagement portion 50 Second Syringe 52 No. 2 Tip 54 Second pusher 56 Second pressing part 57 Second engagement portion 59 Movement Control Department 60 Dispenser 66 Route 1 67 Route 2 68 1st discharge part 69 2nd discharge part 70 1st filling auxiliary part 72 Second closing connection (closing part) 73 1st filling tube tip 80 2nd filling auxiliary part 81 First closing connection (closing part) 83 2nd filling tip 90 Interlocking mechanism 100 Sheet-shaped cell cultures

Claims

1. a first syringe having a first plunger; a second syringe having a second plunger; a first passage communicating with a first cylindrical tip of the first syringe; a first discharge portion communicating with the first path and discharging liquid; a second passage communicating with a second cylindrical tip of the second syringe; a second discharge portion communicating with the second path and discharging liquid, a linking mechanism that links the movement of the second pusher in the pressing direction with the movement of the first pusher in the pressing direction, a pressing portion of the first pusher that is pressed is movable in a pressing direction from a first position to a third position via a second position, the interlocking mechanism causes the liquid to be discharged from the first syringe when the pressing portion moves from the first position to the second position; A discharge device characterized in that liquid is discharged from the second syringe, or from the first syringe and the second syringe, when the pressing portion moves from the second position to the third position.

2. the first pusher has a base end side pusher and a tip end side pusher that is movable further in the tip direction than the base end side pusher, the base end pusher and the tip end pusher have slidable contact surfaces and can move together by frictional force of the contact surfaces, the base end pusher has a first engagement portion, the second pusher has a second engaging portion that can come into contact with and engage with the first engaging portion that moves toward the distal end, The discharge device according to claim 1 , wherein the interlocking mechanism is formed by the base-end pusher having the first engaging portion, the tip-end pusher, and the second pusher having the second engaging portion.

3. 3. The discharge device according to claim 1, wherein a discharge implement having the first path, the first discharge portion, the second path, and the second discharge portion, and a syringe having the first syringe and the second syringe are connectable and detachable.

4. 3. The discharge device according to claim 1, further comprising a first filling auxiliary part including a closing part that closes the second tip of the second syringe and a first filling tip that communicates with the first tip of the first syringe.

5. 3. The discharge device according to claim 1, further comprising a second filling auxiliary part including a closing part that closes the first cylindrical tip of the first syringe and a second filling cylindrical tip that communicates with the second cylindrical tip of the second syringe.

6. 3. The discharge device according to claim 1, wherein the second syringe is arranged concentrically inside the first syringe.

7. The discharge device according to claim 1 or 2, wherein the second syringe is arranged outside and alongside the first syringe.

8. 3. The discharge device according to claim 1, wherein the hole of the second discharge portion is smaller than the hole of the first discharge portion.

9. 9. The discharge device according to claim 8, wherein the number of holes in the second discharge portion is greater than the number of holes in the first discharge portion.

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