Analysis carrier, immunochromatographic test strip, and method of manufacturing analysis carrier

Abstract

To provide an analysis carrier capable of preventing a porous body from separated from an aluminum member even when subjected to bending deformation.SOLUTION: An analytical carrier 1 disclosed herein comprises a porous body 10 and a support 20 supporting the porous body 10 on one surface thereof, the porous body 10 having a skeleton 11 formed by an assembly of multiple hollow particles 13 and multiple voids 12 surrounded by the skeleton 11, each of the hollow particles 13 having an outer shell 14 including an anodic oxide coating containing aluminum oxide, and a cavity 15 surrounded by the outer shell 14. The support 20 includes an adhesive layer 22 adhered to the porous body 10, and the adhesive layer 22 is adhered to the outer shell 14.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present disclosure relates to an analytical carrier, an immunochromatographic test strip, and a method for manufacturing the analytical carrier. [Background technology]

[0002] Immunochromatography (immunochromatography) is a known analytical method for analyzing an analyte carried on an analytical carrier. Lateral flow test kits using immunochromatography are also known. The test kits include a test strip containing a labeled antibody that reacts with the analyte and is labeled with labeling particles such as gold colloid particles and latex particles, and a capture antibody that reacts with the analyte and is immobilized at a predetermined site on the analytical carrier.

[0003] In this test kit, for example, a specimen containing an antigen to be analyzed is collected from a living organism, and a solution containing the specimen is dropped onto a predetermined location on the test kit. The antigen reacts with the labeled antibody to form a complex. The solution containing the complex then flows through the analytical carrier due to capillary action. Furthermore, as the solution containing the complex is developed by the analytical carrier, the antigen contained in the complex is captured by the capture antibody on the analytical carrier, and the complex accumulates at the capture site, causing color development. In this way, the degree of color development at the capture site with the complex supported by the analytical carrier can be visually confirmed to determine whether the specimen contains the antigen.

[0004] Patent Document 1 discloses an aluminum member that includes a porous body including a skeleton formed by an aggregation of a plurality of aluminum particles and a plurality of voids surrounded by the skeleton, the skeleton including an outer shell containing aluminum oxide, and the surface of the skeleton being formed by the outer shell. The aluminum member in Patent Document 1 includes an aluminum substrate that supports the porous body, and the porous body is provided on one or both sides of the substrate. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] International Publication No. 2021 / 079813 Summary of the Invention [Problem to be solved by the invention]

[0006] The aluminum member of Patent Document 1 has high whiteness and water absorption performance, and therefore can be used as an immunochromatography test strip. However, in the aluminum member of Patent Document 1, the porous body is integrally supported by the aluminum substrate. Therefore, when the aluminum member is bent, fracture occurs between the surface of the aluminum substrate and the outer shell of the porous body, and the porous body may peel off from the aluminum member.

[0007] The present disclosure has been made in view of the problems inherent in the conventional techniques, and an object of the present disclosure is to provide an analytical carrier, an immunochromatographic test strip, and a method for manufacturing an analytical carrier, which are capable of preventing a porous body from peeling off from an aluminum member even when subjected to bending deformation. [Means for solving the problem]

[0008] The analytical carrier according to the first aspect of the present disclosure comprises a porous body and a support supporting the porous body on one side. The porous body includes a skeleton formed by an assembly of a plurality of hollow particles and a plurality of voids surrounded by the skeleton. The hollow particles have an outer shell including an anodized film containing aluminum oxide and a cavity surrounded by the outer shell. The skeleton is formed by the continuous outer shells of the plurality of hollow particles. The porosity of the porous body is 50% by volume or more and less than 100% by volume. The average pore diameter of the porous body is 0.1 μm or more and 20 μm or less. The support includes an adhesive layer that adheres to the porous body. The adhesive layer is adhered to the outer shell.

[0009] An immunochromatographic test strip according to a second aspect of the present disclosure includes an analytical carrier.

[0010] A third aspect of the present disclosure provides a method for producing an analytical support comprising a porous body and a support supporting the porous body on one side. The porous body comprises a skeleton formed by an assembly of a plurality of hollow particles and a plurality of voids surrounded by the skeleton. The hollow particles have an outer shell including an anodized film containing aluminum oxide and a cavity surrounded by the outer shell. The skeleton is formed by the continuous outer shells of the plurality of hollow particles. The production method includes a sintering step of sintering a plurality of aluminum metal particles on an aluminum substrate to obtain a sintered material comprising the aluminum substrate and a sintered body in which the aluminum metal particles are stacked on the aluminum substrate. The production method also includes an anodizing step of anodizing the sintered material to form outer shells including an anodized film on the surfaces of the aluminum metal particles. The production method also includes a dissolving step of dissolving the aluminum metal particles surrounded by the outer shells. In the above production method, the anodizing step and the dissolving step are repeated to form a metal member in which a porous body is stacked on an aluminum substrate. The manufacturing method includes a lamination step in which a porous body of a metal member is adhered to a support to form a laminate in which an aluminum substrate, a porous body, and a support are laminated in this order. The manufacturing method also includes a peeling step in which the aluminum substrate is dissolved and peeled off from the laminate. The aluminum metal particles contain at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. The aluminum substrate contains at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. The average particle diameter of the plurality of aluminum metal particles is 0.1 μm or more and 20 μm or less. The packing ratio of the sintered body is 10 vol% or more and 60 vol% or less. The support includes an adhesive layer that adheres to the porous body. In the lamination step, the adhesive layer is adhered to the outer shell. [Effects of the Invention]

[0011] According to the present disclosure, it is possible to provide an analytical carrier, an immunochromatographic test strip, and a method for manufacturing an analytical carrier that can prevent a porous body from peeling off from a support even when subjected to bending deformation. [Brief explanation of the drawings]

[0012] [Figure 1] FIG. 1 is a schematic cross-sectional view showing an example of an analytical carrier according to an embodiment. [Figure 2] FIG. 1 is a perspective view showing an example of a test kit according to an embodiment. [Figure 3] FIG. 1 is a cross-sectional view showing an example of a sintered material obtained by sintering a plurality of aluminum metal particles on an aluminum substrate. [Figure 4] FIG. 2 is a cross-sectional view showing an example of a state in which a sintered material is anodized to form an outer shell including an anodized film on the surface of an aluminum metal particle. [Figure 5] FIG. 2 is a cross-sectional view showing an example of a state in which aluminum metal particles surrounded by an outer shell are dissolved. [Figure 6] FIG. 2 is a cross-sectional view showing an example of a state in which a porous metal member is bonded to a support to form a laminate. [Figure 7] FIG. 2 is a schematic diagram for explaining an example of a first lamination method. [Figure 8] FIG. 10 is a schematic diagram for explaining an example of a second lamination method. [Figure 9] FIG. 10 is a schematic diagram for explaining an example of a third lamination method. [Figure 10] FIG. 10 is a schematic diagram for explaining an example of a fourth lamination method. [Figure 11] FIG. 2 is a cross-sectional view showing an example of a state in which the aluminum base is dissolved and peeled off from the laminate. [Figure 12] 1 is a SEM (scanning electron microscope) image of a cross section of a porous support according to Example 5. [Figure 13] 10 is an SEM image of the surface of a porous support according to Example 5. [Figure 14]1 is a graph showing the relationship between pore diameter and differential pore surface area for Example 5 and a reference example. [Figure 15] 1 is a graph showing the relationship between pore diameter and cumulative pore surface area for Example 5 and a reference example. [Figure 16] FIG. 2 is a plan view schematically showing a state before a bending test is performed. [Figure 17] FIG. 1 is a side view schematically showing a state in which an analytical carrier is bent in a bending test. [Figure 18] FIG. 2 is a plan view schematically illustrating the state of a test strip used in the examples. [Figure 19] 1 is a photograph showing the results of a gold colloid test for Example 5 and a Reference Example. DETAILED DESCRIPTION OF THE INVENTION

[0013] The analytical carrier, immunochromatographic test strip, and analytical carrier manufacturing method according to the present embodiment will be described in detail below with reference to the drawings. The present disclosure is not limited to the following embodiments. Furthermore, some or all of the components in the embodiments can be combined as appropriate. The dimensional proportions in the drawings are exaggerated for the sake of explanation and may differ from the actual proportions.

[0014] [1. Analytical Carrier] First, an analytical carrier 1 according to this embodiment will be described with reference to FIG. 1. FIG. 1 is a schematic cross-sectional view showing an example of the analytical carrier 1. As shown in FIG. 1, the analytical carrier 1 includes a porous body 10 and a support 20 supporting the porous body 10 on one side. If the porous body 10 is supported by an aluminum oxide outer shell integrated with the aluminum oxide on the surface of an aluminum substrate, plastic deformation of the aluminum substrate can cause fracture between the surface of the aluminum substrate and the porous body's outer shell, resulting in the porous body peeling off from the aluminum member. Therefore, the analytical carrier 1 according to this embodiment supports the porous body 10 with the support 20, and the support 20 includes an adhesive layer 22 that adheres to the porous body 10. The adhesive layer 22 is adhered to the outer shell 14, which has been found to prevent the porous body 10 from peeling off from the support 20 even when subjected to bending deformation. The analytical carrier 1 according to this embodiment will be described in detail below.

[0015] <Porous body> The porous body 10 includes a skeleton 11 and a plurality of voids 12 surrounded by the skeleton 11. The skeleton 11 is formed by an aggregation of a plurality of hollow particles 13. The voids 12 are formed by the internal space of the porous body 10 surrounded by the skeleton 11 or a plurality of hollow particles 13. The hollow particles 13 have an outer shell 14 and a cavity 15 surrounded by the outer shell 14. The skeleton 11 includes the outer shell 14, and the surface of the skeleton 11 is formed by the outer shell 14. The skeleton 11 is formed by the continuous outer shells 14 of the plurality of hollow particles 13. The hollow particles 13 are connected via the outer shells 14 to form the skeleton 11 with a three-dimensional network structure. The outer shells 14 are arranged on the outer surface side of the porous body 10.

[0016] The shell 14 of the hollow particle 13 may have a through-hole 16 penetrating the shell 14 in the thickness direction. The through-hole 16 may connect the cavity 15 inside the hollow particle 13 separated by the shell 14 to the void 12 outside the hollow particle 13. The through-hole 16 may also connect the cavities 15 of adjacent hollow particles 13 to each other. The cavities 15 can be in communication with the outside of the hollow particle 13 or the skeleton 11 through the through-hole 16. When the porous body 10 is impregnated with a liquid containing a specimen or the like, the liquid can flow into or out of the cavities 15 through the through-hole 16 in the shell 14. Furthermore, the cavities 15 in adjacent hollow particles 13 are in communication. Therefore, when the porous body 10 is impregnated with a liquid, the liquid can pass through the through-hole 16 and the cavities 15, thereby flowing inside the skeleton 11.

[0017] Here, in immunochromatography, as described below, labels such as colored particles, gold colloid particles, or fluorescent beads are typically used. When the analytical carrier 1 is used, for example, in an immunochromatographic test strip, the porous body 10 can absorb a solution in which the label is dispersed by capillary action. Although the mechanism behind this is unclear, it is presumed that the solution in which the label is dispersed penetrates the voids 12 and cavities 15 present inside the porous body 10, and that a portion of the solution flows along the surface of the porous body 10. Furthermore, while absorption is primarily achieved by the solution flowing through the voids 12, it is presumed that the flow of the solution through the cavities 15 and through-holes 16 also contributes to absorption.

[0018] In this way, the porous body 10 is a porous body having voids 12 therein that communicate with the outside. In this case, the voids 12 are surrounded by the outer shell 14. That is, the voids 12 are formed by being surrounded by the skeleton 11 inside the porous body 10 or the outer shell 14 formed on its surface. The voids 12 forming one cell structure surrounded by the outer shell 14 may be connected to the voids 12 forming another cell structure. Specifically, the porous body 10 may have an open-cell structure. Furthermore, the single void 12 or multiple voids 12 may or may not penetrate from one surface of the porous body 10 to the other surface.

[0019] The average particle diameter of the plurality of hollow particles 13 is 0.1 μm to 20 μm. By setting the average particle diameter to 0.1 μm or more, the strength of the porous body 10 is improved, and breakage during transportation, etc. can be suppressed. By setting the average particle diameter to 20 μm or less, the flow rate of the analytical carrier 1 can be improved. The average particle diameter may be 1 μm or more, 3 μm or more, or 5 μm or more. The average particle diameter may be 18 μm or less, 15 μm or less, or 10 μm or less.

[0020] The average particle diameter of the plurality of hollow particles 13 can be measured by observing the cross section of the porous body 10 using a scanning electron microscope. For example, after sintering, aluminum metal particles are partially melted and connected, but the portions having a substantially circular shape can be considered approximately circular. Therefore, in the above cross-sectional observation, the maximum diameter (longest diameter) of each of the substantially circular aluminum metal particles is taken as the particle diameter, and the particle diameters of 50 randomly selected aluminum metal particles are measured. The arithmetic average of these particles is taken as the average particle diameter of the aluminum metal particles after sintering. The average particle diameter of the plurality of aluminum metal particles before sintering is the D50 value obtained by measuring the particle size distribution on a volume basis using a laser diffraction method. Note that if the diameter is within the range of 0.1 μm to 20 μm, almost no secondary particles of the aluminum metal particles are formed. Therefore, the average particle diameter of the hollow particles 13 after sintering observed using a scanning electron microscope is approximately the same as the average particle diameter of the aluminum metal particles before sintering measured using a laser diffraction method.

[0021] The shape of each hollow particle 13 is not particularly limited and may be spherical, polygonal, irregular, scale-like, fibrous, or the like. Among these, the hollow particles 13 are preferably spherical. When the hollow particles 13 are spherical, the sizes of the multiple voids 12 are uniform. For example, in immunochromatography, as described below, labels such as colored particles, gold colloid particles, or fluorescent beads are used. Furthermore, when the sizes of the voids 12 are uniform, such labels are not trapped within the voids 12 and can flow smoothly through the porous body 10. The term "spherical" as used herein does not only refer to perfect spheres, but also to particles with slight surface irregularities. Furthermore, the term "spherical" as used herein does not only refer to hollow particles 13 with an aspect ratio of 1, but also includes hollow particles 13 with an aspect ratio of 1 or more. The aspect ratio of the hollow particles 13 may be 5 or less, 3 or less, or 2 or less. The aspect ratio is the ratio of the major axis to the minor axis of the hollow particles 13 and is the average value of multiple hollow particles 13.

[0022] The outer shell 14 includes an anodized film containing aluminum oxide. The anodized film included in the outer shell 14 may be a barrier-type anodized film or a porous-type anodized film. The outer shell 14 may be formed solely of a barrier-type anodized film, or may be formed of a two-layer anodized film in which a porous-type anodized film is formed on the outer surface of the barrier-type anodized film. The outer shell 14 may have a hydrated film containing aluminum hydroxide. The hydrated film is formed in a hydration treatment process described below.

[0023] The ratio of metallic aluminum to the components constituting porous body 10 containing elemental aluminum may be 0 or more and 0.1 or less in mass ratio. In other words, the ratio of the mass of metallic aluminum contained in porous body 10 to the mass of the components constituting porous body 10 containing elemental aluminum may be 0 or more and 0.1 or less in mass ratio. The mass of the components constituting porous body 10 containing elemental aluminum refers to the total mass of aluminum oxide, aluminum hydroxide, electrolyte anions, resin residue (burnt residue), and metallic aluminum contained in porous body 10. By setting the ratio of metallic aluminum to 0.1 or less, the light transmittance of porous body 10 is increased even when porous body 10 absorbs water, thereby reducing the gray color caused by metallic aluminum. This improves the distinguishability of water absorption. The ratio of metallic aluminum is preferably 0.05 or less, more preferably 0.01 or less, and even more preferably 0.001 or less. The ratio of metallic aluminum to the components constituting porous body 10 containing aluminum element contained in porous body 10 can be calculated based on JIS G2404: 2022. Note that outer shell 14 of hollow particle 13 may consist essentially of an anodized film containing aluminum oxide.

[0024] The thickness of the shell 14 is preferably 40 nm to 1000 nm. By making the thickness of the shell 14 40 nm or more, damage to the porous body 10 can be suppressed even when the analytical carrier 1 is bent. This makes it easier to prevent the outer shell 14 from being broken and leaving wrinkles when the analytical carrier 1 is subjected to bending deformation. Furthermore, by setting the thickness of the shell 14 within this range, an analytical carrier 1 with sufficiently high corrosion resistance can be provided. The thickness of the outer shell 14 may be 100 nm or more, 200 nm or more, or 400 nm or more. The thickness of the outer shell 14 may be 900 nm or less, 700 nm or less, or 500 nm or less. When the thickness of the outer shell 14 is equal to or less than the above upper limit, when the outer shell is formed by alternately repeating the anodizing step and the dissolution step, the aluminum portion of the metal can be dissolved to form an outer shell of the desired thickness without forming an excessively thick anodized film, which makes it easier to improve production efficiency. The thickness of the outer shell 14 can be measured, for example, by observing a cross section of the outer shell 14 with a scanning electron microscope or the like.

[0025] The cumulative pore surface area of ​​the porous body 10 having a pore diameter of 0.1 μm or more and 100 μm or less is 0.1 m 2 / cm 3 More than 20m 2 / cm 3 The porous body 10 may have an integrated pore surface area of ​​0.1 m or less. 2 / cm 3 When the cumulative pore surface area of ​​the porous body 10 is 20 m or more, the amount of antibody adsorbed to the porous body 10 increases. This makes it possible to improve the absorbance of the analytical carrier 1. 2 / cm 3 When the cumulative pore surface area is 0.5 m or less, the water absorption of the porous body 10 can be improved. 2 / cm 3 It may be more than 1m 2 / cm 3 The cumulative pore surface area may be 10 m or more. 2 / cm 3 May be less than 6m 2 / cm 3The cumulative pore surface area of ​​the porous body 10 can be obtained by converting the pore volume of pores having a pore diameter of 0.1 μm or more and 100 μm or less, measured by mercury intrusion porosimetry, into a surface area for each pore diameter and then integrating the converted volume.

[0026] The porosity of the porous body 10 is 50% by volume or more and less than 100% by volume. By setting the porosity of the porous body 10 to 50% by volume or more, the water absorption performance can be improved. By setting the porosity to less than 100% by volume, peeling of the hollow particles 13 from the porous body 10 can be suppressed, and the strength of the porous body 10 can be improved. The porosity may be 60% by volume or more, 70% by volume or more, 80% by volume or more, 90% by volume or more, or 95% by volume or more. The porosity may also be 95% by volume or less, 90% by volume or less, or 80% by volume or less. The porosity of the porous body 10 can be obtained by dividing the cumulative pore volume of the porous body 10 by the volume of the porous body 10. The cumulative pore volume of the porous body 10 can be obtained by integrating the pore volumes of pores having a pore diameter of 0.1 μm or more and 100 μm or less obtained by mercury intrusion porosimetry.

[0027] The average pore diameter of the porous body 10 is 0.1 μm or more and 20 μm or less. By setting the average pore diameter to 0.1 μm or more, the water wicking performance of the analytical carrier 1 can be improved. Here, the particle diameter of typical markers used in immunochromatography, such as colored particles, gold colloid particles, and fluorescent beads, is approximately 10 nm to 50 nm, and markers with larger particle diameters also exist. When the analytical carrier 1 is used, for example, in an immunochromatographic test strip, setting the average pore diameter to 0.1 μm or more prevents such markers from being trapped in the voids 12, allowing the markers to flow smoothly through the porous body 10. Furthermore, by setting the average pore diameter to 20 μm or less, the water wicking performance of the analytical carrier 1 can be improved. The average pore diameter may be 0.5 μm or more, 1 μm or more, 3 μm or more, or 5 μm or more. The average pore diameter may also be 15 μm or less, 12 μm or less, 10 μm or less, or 9 μm or less. The average pore diameter of the porous body 10 can be calculated by the following formula. Average pore diameter of porous body 10=4×(cumulative pore volume of porous body 10) / (cumulative pore surface area of ​​porous body 10) The cumulative pore volume of the porous body 10 and the cumulative pore surface area of ​​the porous body 10 can be obtained as described above.

[0028] The ratio of the integral pore surface area of ​​pores having a diameter of 1 μm or more and less than 1 μm to the integral pore surface area of ​​pores having a diameter of 1 μm or more and less than 10 μm may be 0.1 or more and 10 or less. The ratio of the integral pore surface areas may be 0.2 or more, 0.5 or more, or 1 or more. The ratio of the integral pore surface areas may be 8 or less, 6 or less, or 5 or less. The integral pore surface area of ​​pores having a diameter of 1 μm or more and less than 10 μm can be obtained by integrating the pore surface areas of pores having a diameter of 1 μm or more and less than 10 μm. Similarly, the integral pore surface area of ​​pores having a diameter of 0.1 μm or more and less than 1 μm can be obtained by integrating the pore surface areas of pores having a diameter of 0.1 μm or more and less than 1 μm. The integral pore surface area of ​​the porous body 10 can be determined by mercury intrusion porosimetry as described above. Signals such as absorbance from the analytical carrier 1 tend to be proportional to the flow rate. Furthermore, to lower the flow rate, in other words, to increase the water absorption rate, it is necessary to increase the porosity. In other words, there is a trade-off between a high porosity and a high water absorption rate analytical carrier 1, resulting in a lower signal. When the cumulative pore surface area ratio is equal to or greater than the lower limit, the surface area of ​​the porous body 10 increases, increasing the amount of antibody bound to the porous body 10 and resulting in a stronger signal from the analytical carrier 1. Even if the analytical carrier 1 has a flow rate similar to that of a nitrocellulose membrane, a higher absorbance than a nitrocellulose membrane can be obtained by setting the cumulative pore surface area ratio equal to or greater than the lower limit. Setting the cumulative pore surface area ratio equal to or less than the upper limit can prevent clogging of labeled particles.

[0029] The thickness of the porous body 10 is preferably 20 μm or more and 190 μm or less. By making the thickness of the porous body 10 20 μm or more, it becomes easier to ensure a sufficient thickness for absorbing water by capillary action. By making the thickness of the porous body 10 190 μm or less, it is possible to prevent the analytical carrier 1 from appearing gray when the porous body 10 absorbs water. Furthermore, by making the thickness of the porous body 10 190 μm or less, it is possible to prevent the porous body 10 from cracking and forming cracks when the analytical carrier 1 is bent, which can result in wrinkles remaining. The thickness of the porous body 10 may be 30 μm or more, 40 μm or more, or 50 μm or more. The thickness of the porous body 10 may be 150 μm or less, or 100 μm or less.

[0030] The porous body 10 may be composed of a single porous layer, or may include multiple porous layers. The porous body 10 may include, for example, a first porous layer and a second porous layer disposed on the surface of the first porous layer. The first and second porous layers may differ in any of the average particle size, aspect ratio, or constituent material of the multiple hollow particles 13, the porosity, or average pore size of the porous body 10, etc. For example, the average particle size of the hollow particles 13 contained in the first porous layer may be larger than the average particle size of the hollow particles 13 contained in the second porous layer. Furthermore, the thicknesses of the first and second porous layers may be the same or different.

[0031] The porous body 10 may include a coating layer 17, which is provided on the surface opposite the support 20 and separates the inside of the porous body 10, where the skeleton 11 and voids 12 are present, from the outside of the porous body 10. The coating layer 17 may have communicating pores 18 connecting the inside and outside of the porous body 10. The coating layer 17 may be formed continuously with the outer shells 14 of the hollow particles 13. The cavities 15 of the hollow particles 13 may be connected to the outside of the porous body 10 through the communicating pores 18. The inclusion of the coating layer 17 in the porous body 10 prevents the hollow particles 13 from being exposed to the outside, preventing direct contact with the porous body 10 by fingers or the like. This prevents the hollow particles 13 from peeling off. This improves the handleability of the analytical carrier 1.

[0032] The surface roughness Sa of the porous body 10 may be 0.01 μm or more and less than 1.8 μm. When the surface roughness Sa of the porous body 10 is less than 1.8 μm, the hollow particles 13 are not exposed on the surface of the porous body 10, and the surface of the porous body 10 tends to be covered by the coating layer 17. This makes it difficult for the hollow particles 13 of the porous body 10 to peel off from the porous body 10, the support 20, or the analytical carrier 1. The surface roughness Sa of the porous body 10 may be 0.1 μm or more, 0.2 μm or more, 0.4 μm or more, 0.6 μm or more, or 0.8 μm or more. The surface roughness Sa of the porous body 10 may be 1.5 μm or less, 1.2 μm or less, or 1 μm or less. The arithmetic mean roughness Sa can be obtained by measuring the surface of the porous body 10 opposite the support 20 in accordance with ISO 25178.

[0033] <Support> The support 20 supports the porous body 10 on one side. By supporting the porous body 10 with the support 20, it is possible to prevent the porous body 10 from being damaged due to deformation or the like. The support 20 includes an adhesive layer 22 that adheres to the porous body 10. The adhesive layer 22 adheres to the outer shell 14 of the porous body 10.

[0034] The support 20 may include an adhesive layer 22 that adheres to the porous body 10, and a support layer 21 that supports the adhesive layer 22. In this case, the adhesive layer 22 is interposed between the porous body 10 and the support layer 21. By adhering the porous body 10 and the support layer 21 with the adhesive layer 22, the porous body 10 and the support layer 21 can be firmly bonded together. Alternatively, the support 20 may not include the support layer 21, and the support 20 may be constituted by the adhesive layer 22.

[0035] The adhesive layer 22 has adhesive sections 23 in which the material constituting the adhesive layer 22 penetrates the voids 12 and cavities 15 of the porous body 10, comes into contact with the hollow particles 13 and the outer shells 14, and adheres to the hollow particles 13 and the outer shells 14. The adhesive sections 23 are portions in which the material constituting the adhesive layer 22 and the hollow particles 13 and the outer shells 14 are mixed along the adhesive interface direction between the porous body 10 and the adhesive layer 22.

[0036] The adhesive layer 22 may have an adhesive portion 23 and an auxiliary portion 24. The auxiliary portion 24 is made of the material that constitutes the adhesive layer 22 and is continuous with the adhesive portion 23. It supports adhesion by lining the adhesive portion 23 from the side opposite the porous body 10. The auxiliary portion 24 is a portion formed by the material that constitutes the adhesive layer 22 along the adhesive interface direction between the porous body 10 and the adhesive layer 22. The auxiliary portion 24 is provided on the adhesive layer 22 on the side opposite the adhesive portion 23 that is in contact with the porous body 10. The auxiliary portion 24 is disposed between the adhesive portion 23 and the support layer 21, and by bonding the auxiliary portion 24 to the support layer 21, it also functions as a layer that bonds the adhesive portion 23 to the support layer 21. The auxiliary portion 24 also functions as a layer that bonds the adhesive layer 22 to the support layer 21.

[0037] For example, when a thermoplastic resin is used as the adhesive layer 22 to bond the adhesive layer 22 and the porous body 10, when the heated adhesive layer 22 comes into contact with the porous body 10, the softened thermoplastic resin in contact with the porous body 10 deforms and enters the voids 12 and cavities 15 of the porous body 10, and hardens as the temperature drops. At this time, the thermoplastic resin enters the voids 12 and cavities 15 of the porous body 10, and the layer portion in contact with the thermoplastic resin, hollow particles 13, and shells 14 is mixed and in contact with the thermoplastic resin is called the adhesive portion 23. On the other hand, when the adhesive layer 22 and the porous body 10 are bonded, the portion of the thermoplastic resin that does not come into contact with the porous body 10 and remains a thermoplastic resin layer is called the auxiliary portion 24.

[0038] Furthermore, for example, when an adhesive (pressure-sensitive adhesive) is used as the adhesive layer 22 to bond the adhesive layer 22 and the porous body, when the adhesive layer 22 comes into contact with the porous body 10, the portion of the adhesive that comes into contact with the porous body 10 deforms and enters the voids 12 and cavities 15 of the porous body 10. At this time, as the adhesive enters the voids 12 and cavities 15 of the porous body 10, the layer portion where the adhesive, the hollow particles 13, and the shells 14 are mixed and in contact is called the adhesive portion 23. On the other hand, when the adhesive layer 22 and the porous body 10 are bonded, the portion of the adhesive that does not come into contact with the porous body 10, where the adhesive layer is maintained, and which is continuous with the adhesive portion 23 is called the auxiliary portion 24.

[0039] The thickness of the adhesive layer 22 may be 1 μm or more, 5 μm or more, 10 μm or more, 20 μm or more, or 30 μm or more. The thickness of the adhesive layer 22 may also be 1000 μm or less, 500 μm or less, 300 μm or less, 200 μm or less, 100 μm or less, 50 μm or less, or 40 μm or less. The thickness of the adhesive layer 22 is usually greater than the thickness of the adhesive portion 23. When the thickness of the adhesive layer 22 is equal to or greater than the above-mentioned lower limit, the adhesive layer 22 adheres to the porous body 10, and when the support 20 is deformed, the adhesive layer 22 alleviates the deformation, thereby suppressing strain generated between the support 20 and the porous body 10, thereby making it easier to maintain the adhesion between the support 20 and the porous body 10. This makes it easier to prevent the porous body 10 from peeling off from the support 20 when the support 20 is deformed. Furthermore, it makes it easier to prevent wrinkles from occurring in the porous body 10 when the support 20 is deformed. When the thickness of the adhesive layer is equal to or less than the upper limit, the adhesive layer can bend appropriately during roll-to-roll production, making it easy to transport. The thickness of the adhesive layer 22 refers to the overall thickness in the thickness direction of the material constituting the adhesive layer 22 when the cross section is observed in a plane perpendicular to the adhesive interface direction between the porous body 10 and the adhesive layer 22.

[0040] The thickness of the adhesive portion 23 may be 1 μm or more, 2 μm or more, 3 μm or more, 4 μm or more, 5 μm or more, or 10 μm or more. The thickness of the adhesive portion 23 may also be 100 μm or less, 50 μm or less, 40 μm or less, 30 μm or less, 20 μm or less, or 10 μm or less. When the thickness of the adhesive portion 23 is equal to or greater than the above-mentioned lower limit, the adhesive portion 23 contacts the outer shell 14 with a sufficient thickness that is equal to or greater than the thickness of the outer shell 14. In this case, the adhesive portion 23 penetrates deep into the outer shell 14, thereby exerting an anchoring effect and facilitating strong bonding between the porous body 10 and the support 20. Furthermore, the increased contact area between the outer shell 14 and the adhesive portion 23 strengthens the effects of hydrogen bonding, van der Waals forces, ionic bonding, or covalent bonding by the adhesive portion 23, thereby facilitating strong bonding between the porous body 10 and the support 20. This makes it easier to prevent the porous body 10 from peeling off from the support 20 when the support 20 is deformed. Furthermore, it makes it easier to prevent wrinkles from occurring in the porous body 10 when the support 20 is deformed. Having the thickness of the adhesive portion 23 equal to or less than the above upper limit value makes it possible to prevent the porous body 10 from being completely filled with resin. The thickness of the adhesive portion 23 refers to the distance between a line passing through the portion of the porous body 10 closest to the adhesive layer 22 (the bottom) and parallel to the adhesive interface direction between the porous body 10 and the adhesive layer 22, and a line passing through the portion of the adhesive layer 22 closest to the porous body 10 (the top) and parallel to the adhesive interface direction between the porous body 10 and the adhesive layer 22, in the area where the material constituting the adhesive layer 22 is in contact with the porous body 10, when observed in cross section in a plane perpendicular to the adhesive interface direction between the porous body 10 and the adhesive layer 22.

[0041] The thickness of the support layer 21 may be 10 μm or more, 50 μm or more, 70 μm or more, 90 μm or more, or 100 μm or more, or 1000 μm or less, 500 μm or less, 300 μm or less, 200 μm or less, 150 μm or less, or 100 μm or less.

[0042] The support 20 may be flexible. When a porous body is disposed on the surface of an aluminum substrate, such as the aluminum member described in Patent Document 1, bending the analytical support 1 may cause the aluminum substrate to plastically deform and not return to its original shape. In this case, the porous body disposed on the surface of the aluminum substrate may also wrinkle in response to the bending of the aluminum substrate. On the other hand, when the analytical support 1 is flexible, the analytical support 1 is more likely to return to its original shape even after bending deformation, making the support 20 less likely to wrinkle. The support 20 supports the porous body 10, and wrinkles are less likely to form on the support 20, which can prevent wrinkles from forming on the porous body 10 of the analytical support 1.

[0043] The adhesive layer 22 may contain at least one selected from the group consisting of resin, elastomer, starch, and protein. That is, the adhesive layer 22 may be a resin adhesive layer or an elastomer adhesive layer. The resin may contain at least one selected from the group consisting of a thermoplastic resin, a thermosetting resin, and a UV-curable resin. The UV-curable resin may contain at least one of a radical-curable resin and a cation-curable resin. The resin may contain at least one selected from the group consisting of polyolefins such as polyethylene (HDPE, MDPE, LDPE), polypropylene, modified polyolefin resins (modified polypropylene, modified polyethylene, etc.), ethylene-vinyl acetate copolymers, polyvinyl chloride, polyvinyl acetate, polyamide, polyester, (meth)acrylic resin, epoxy resin, urethane resin, fluororesin, styrene resin, ABS resin, silicone resin, phenolic resin, melamine resin, polyvinyl alcohol, and polycyanoacrylate. The resin may include adhesives (pressure-sensitive adhesives) such as isocyanate-based adhesives, urethane resin-based adhesives, urethane resin solvent-based adhesives, urethane resin emulsion adhesives, acrylic resin-based adhesives, anaerobic acrylic resin adhesives, olefin-based adhesives, silicone-based adhesives, rubber-based adhesives, ethylene vinyl acetate resin emulsion adhesives, epoxy resin-based adhesives, epoxy resin emulsion adhesives, vinyl chloride resin solvent-based adhesives, chloroprene rubber-based adhesives, cyanoacrylate-based adhesives, aqueous polymer-isocyanate-based adhesives, styrene-butadiene rubber solution-based adhesives, styrene-butadiene rubber latex adhesives, nitrile rubber-based adhesives, nitrocellulose adhesives, phenolic resin-based adhesives, modified silicone-based adhesives, polyimide-based adhesives, polystyrene resin solvent-based adhesives, polybenzimidazole adhesives, polymethacrylate resin solution-based adhesives, and photo- or UV-curable resin-based adhesives. The elastomer may include at least one selected from the group consisting of thermoplastic elastomers and rubbers. The adhesive layer 22 may include an ethylene vinyl acetate copolymer. Ethylene vinyl acetate copolymer melts at a relatively low temperature, so that the porous body 10 and the support layer 21 can be easily bonded together.When the adhesive layer 22 contains a thermoplastic resin, the melting point of the adhesive layer 22 is preferably lower than the melting point of the support 20. From the viewpoint of suppressing deformation of the support 20 when the analytical carrier 1 is subjected to bending deformation, the adhesive layer 22 preferably contains a highly flexible material.

[0044] The support layer 21 may contain at least one material selected from the group consisting of resin, elastomer, metal, and paper. These materials can effectively support the porous body 10. From the viewpoint of suppressing deformation of the support 20 when the analytical carrier 1 is subjected to bending deformation, the support layer 21 preferably contains a material that is resistant to plastic deformation when a certain strain is applied. Furthermore, the L measured when the analytical carrier 1 with water absorbed into the porous body 10 is placed on a white reflection standard is * From the viewpoint of improving the optical transmittance, the support layer 21 preferably contains a material with high light transmittance or high whiteness. The resin may contain at least one selected from the group consisting of a thermoplastic resin, a thermosetting resin, and a UV-curable resin. The resin may contain at least one selected from the group consisting of a polyester, an acrylic resin, a urethane resin, a silicone resin, an epoxy resin, a polyolefin, a cellulose resin, a styrene resin, a polyimide, and a polycarbonate. The elastomer may contain at least one selected from the group consisting of a thermoplastic elastomer and a rubber. The metal may contain at least one selected from the group consisting of aluminum, iron, copper, gold, silver, and zinc, for example.

[0045] The support layer 21 may be a single layer or may be multi-layered. When the support layer 21 is multi-layered, the materials of the layers may be the same or different. When the support layer 21 is multi-layered, it is preferable that the melting point of the material of the support layer 21 arranged closest to the adhesive layer 22 is lower than the melting point of the material of the support layer 21 arranged on the opposite side of the adhesive layer 22. The support layer 21 may be, for example, two layers of LDPE (low-density polyethylene) and PET (polyethylene terephthalate). By arranging the layers in this way, it is possible to prevent the adhesive layer 22 from protruding during lamination.

[0046] The resin contained in the support layer 21 may contain a filler. The filler may contain at least one of an organic filler and an inorganic filler. The organic filler may contain at least one selected from the group consisting of a resin filler, a cellulose nanofiber, and a pigment. The inorganic filler may contain at least one selected from the group consisting of an oxide, a hydroxide, a carbide, a nitride, a boride, a silicide, and a fluoride. The filler may be spherical, polygonal, irregular, scaly, needle-like, or fibrous. The support layer 21 may be in the form of a plate, a sheet, a woven fabric, or a nonwoven fabric.

[0047] <Analytical carrier> The analytical carrier 1 includes a porous body 10 and a support 20. The support 20 includes an adhesive layer 22. The support 20 may include the adhesive layer 22 and a support layer 21. The adhesive layer 22 of the support 20 is bonded to the outer shell 14 of the porous body 10. The adhesive layer 22 may be bonded to the outer shell 14, and the porous body 10 and the support 20 may be laminated by bonding together with the adhesive layer 22 interposed between the porous body 10 and the support layer 21. The adhesive layer 22 may include an adhesive portion 23 and an auxiliary portion 24. The support 20 may include the adhesive layer 22 including the adhesive portion 23 and the auxiliary portion 24, and the support layer 21, and the adhesive portion 23, auxiliary portion 24, and support layer 21 may be laminated together in this order. Since the adhesive portion 23 and the outer shell 14 are bonded together and the auxiliary portion 24 and the support layer 21 are bonded together, the porous body 10, the adhesive portion 23, the auxiliary portion 24, and the support layer 21 may be laminated in this order.

[0048] The adhesive layer 22 may be bonded to the shell 14 located on the surface of the porous body 10 opposite the coating layer 17. The porous body 10 may have the shell 14 bonded to the adhesive layer 22, and the coating layer 17 located on the surface opposite the shell 14 bonded to the adhesive layer 22 may be exposed. In the analytical carrier 1, when the porous body 10 and the support 20 are bonded together, the coating layer 17 may be provided on the side opposite the support 20 and located outside the porous body 10, and the hollow particles 13 and the shells 14 may be located inside the porous body 10 sandwiched between the coating layer 17 and the support 20. In this way, in the analytical carrier 1, the coating layer 17 is located on the outside of the porous body 10, and the hollow particles 13 and the shells 14 are located inside the porous body 10, and the hollow particles 13 and the shells 14 are covered by the coating layer 17 provided on the surface layer of the analytical carrier 1. This prevents the hollow particles 13 from peeling off from the porous body 10, the support 20, or the analytical carrier 1, making it easier to handle.

[0049] The thickness of the analytical carrier 1 may be, for example, 20 μm or more and 10 cm or less, depending on the application. By setting the thickness of the analytical carrier 1 within this range, an analytical carrier 1 with good bending strength can be provided. The thickness of the analytical carrier 1 may be 30 μm or more, 50 μm or more, 100 μm or more, or 150 μm or more. The thickness of the analytical carrier 1 may be 1000 μm or less, 300 μm or less, or 200 μm or less.

[0050] The porous body 10 is provided on a first surface, which is one surface of the support 20, and the second surface, which is the surface opposite to the first surface, may be exposed. With this configuration, the analytical carrier 1 can improve problems caused by reattaching the porous body 10. Specifically, when preparing an immunochromatographic test strip or test kit, an adhesive member constituting the immunochromatographic test strip or test kit is attached to the support 20. However, when attempting to peel off the adhesive member attached to the support 20 from the support 20 and then reattach it for position adjustment or the like, it may be difficult to reattach it.

[0051] For example, when the porous body 10 is provided on both sides of an aluminum substrate (described later), if an attachment member is attached to the porous body 10 provided on one side of the aluminum substrate and then the attachment member is peeled off, the porous body 10 may peel off from the aluminum substrate. In such cases, the porous body 10 remains on the attachment member, which may weaken the adhesive strength of the attachment member. Furthermore, when the porous body 10 is provided on one side of the aluminum substrate, if the attachment member is attached to the side of the aluminum substrate where the porous body 10 is not provided and then the attachment member is peeled off, the aluminum substrate may undergo plastic deformation. In such cases, wrinkles may occur in the porous body 10.

[0052] On the other hand, when the porous body 10 is provided on the first surface of the support 20 and the second surface of the support 20 is exposed, the above-mentioned problems caused by re-attachment can be suppressed by attaching an attachment member to the support 20. The first surface and the second surface may be the main surfaces. Here, the main surfaces are surfaces perpendicular to the thickness direction of the support 20, and are the two surfaces with the first and second largest areas among the six surfaces that form the support 20.

[0053] The analytical carrier 1 with the porous body 10 absorbing water is placed on a white reflection standard with which the measuring instrument is calibrated, and the surface of the porous body 10 in the analytical carrier 1 is measured with the measuring instrument. * a * b * L in color space * The value may be 75 or more. * By setting the value to 75 or more, it is possible to improve the discrimination ability when water is absorbed. * The value may be 80 or more, 85 or more, 90 or more, or 95 or more. * a * b * L in color space *The L value can be measured using a color difference meter with a 45° circular illumination and vertical light receiving method conforming to JIS Z8722. The analytical carrier 1 with water absorbed into the porous body 10 can be obtained by making the porous body 10 absorb pure water. The L value when a white reflection standard with which the measuring instrument is calibrated is measured with the measuring instrument can be measured. * a * b * L in color space * The value is 97.1.

[0054] The analytical carrier 1 with the dried porous body 10 is placed on a white reflection standard with which the measuring instrument is calibrated, and the surface of the porous body 10 in the analytical carrier 1 is measured with the measuring instrument. * a * b * L in color space * The value may be 75 or more. * By setting the value to 75 or more, it is possible to improve the discrimination ability when water is absorbed. * The value may be 80 or more, 85 or more, 90 or more, or 95 or more. Note that the porous body 10 being dry means that the porous body 10 has not absorbed any water.

[0055] The analytical carrier 1 with the porous body 10 absorbing water is placed on a white reflection standard with which the measuring instrument is calibrated, and the surface of the porous body 10 in the analytical carrier 1 is measured with the measuring instrument. * a * b * L in color space * The value may be less than 75. In addition, the analytical carrier 1 with the dried porous body 10 is placed on a white reflection standard with which the measuring instrument is calibrated, and the L * a * b * L in color space * The value may be less than 75. By adopting such a configuration, even if fluorescence is emitted from the backing sheet to which the analytical carrier 1 is attached, L *Since the L value is less than 75, it becomes difficult for the fluorescent light to pass through. Therefore, it is possible to prevent the background from increasing and the SN ratio from decreasing when measuring the fluorescent light. * The value may be 70 or less, 60 or less, 50 or less, 40 or less, 30 or less, 20 or less, or 10 or less.

[0056] It is preferable that the time required for the analytical carrier 1 to draw up water to a height of 4 cm by capillary action is 400 seconds or less. By doing so, it is possible to provide an analytical carrier 1 suitable for, for example, chromatography. The time may be 20 seconds or more, 40 seconds or more, or 50 seconds or more. The time may also be 360 ​​seconds or less, 200 seconds or less, or 100 seconds or less. The time can be obtained, for example, by immersing the analytical carrier 1 in pure water at room temperature (30°C) so that the planar direction of the analytical carrier 1 is perpendicular to the liquid surface, and measuring the time required for water to be drawn up to a height of 4 cm by capillary action. The pure water has a resistivity of 10 kΩ-m measured at 30°C.

[0057] As shown in FIGS. 16 and 17 , it is preferable that the analytical carrier 1 does not experience peeling of the porous body 10 during a bending test. It is also preferable that the analytical carrier 1 does not leave wrinkles on the surface of the porous body 10 during a bending test. Specifically, in the bending test, a 10 g weight 230 is attached to each end of an analytical carrier 1 cut into a 10 mm x 100 mm piece. The analytical carrier 1 is then placed on a stainless steel rod 240 at the longitudinal center of the analytical carrier 1 so that the porous body 10 is in contact with the stainless steel rod 240. The analytical carrier 1 is then lifted until the weights 230 at both ends of the analytical carrier 1 float, and held for 10 seconds. After holding for 10 seconds, the stainless steel rod 240 is returned to its original position, and the surface of the porous body 10 is visually observed and evaluated. An analytical carrier 1 that does not experience peeling of the porous body 10 during a bending test is easier to handle because the porous body 10 is less likely to peel when the analytical carrier 1 is bent or reattached. Furthermore, an analytical carrier 1 that does not leave wrinkles on the surface of the porous body 10 in a bending test is less likely to leave wrinkles when bent or reattached, making it easier to handle the analytical carrier 1. Peeling of the porous body 10 refers to separation of the adhesive layer 22 and the porous body 10, and refers to a situation in which the minimum width of the separated portion within the surface of the analytical carrier 1 is 2 mm or more and the maximum length is 5 mm or more. Wrinkles on the porous body 10 refer to wrinkles of millimeter order size that can be visually confirmed.

[0058] <Action and effect> As described above, the analytical carrier 1 according to this embodiment includes a porous body 10 and a support 20 that supports the porous body 10 on one side. The porous body 10 includes a skeleton 11 formed by an aggregation of a plurality of hollow particles 13, and a plurality of voids 12 surrounded by the skeleton 11. The hollow particles 13 have an outer shell 14 that includes an anodized film containing aluminum oxide, and a cavity 15 surrounded by the outer shell 14. The skeleton 11 is formed by the continuous outer shells 14 of the plurality of hollow particles 13. The porosity of the porous body 10 is 50% by volume or more and less than 100% by volume. The average pore diameter of the porous body 10 is 0.1 μm or more and 20 μm or less. The support 20 includes an adhesive layer 22 that adheres to the porous body 10, and the adhesive layer 22 adheres to the outer shell 14.

[0059] In an aluminum component such as that disclosed in Patent Document 1, in which a porous body is supported by an aluminum substrate and the aluminum oxide of the porous body is integrated with the aluminum oxide of the aluminum substrate, when the aluminum component is subjected to bending deformation, the portion of the aluminum oxide connecting the porous body to the aluminum substrate breaks, and the porous body may peel off from the aluminum substrate.

[0060] The analytical carrier 1 according to this embodiment includes a support 20 that supports the porous body 10. The support 20 includes an adhesive layer 22 that adheres to the porous body 10, and the adhesive layer 22 is adhered to the outer shell 14. In this manner, the porous body 10 is supported by the support 20 while being adhered by the adhesive layer 22. Therefore, when the analytical carrier 1 is subjected to bending deformation, the adhesive layer 22 alleviates deformation (distortion) that occurs between the porous body 10 and the support 20, making it easier to avoid breakage between the porous body 10 and the support 20. Therefore, the analytical carrier 1 according to this embodiment can prevent the porous body 10 from peeling off from the support 20 even when subjected to bending deformation.

[0061] [2. Immunochromatography test strips] The immunochromatographic test strip includes an analytical carrier 1. The analytical carrier 1 can analyze the analyte while supporting the analyte. The analytical carrier 1 can prevent the porous body 10 from peeling off from the support 20 even when subjected to bending deformation, and therefore can be suitably used as an immunochromatographic test strip. The immunochromatographic test strip is also called an immunochromatographic developing member, a lateral flow assay test strip, or a lateral flow assay developing member. The analytical carrier 1 is also preferably used in in vitro diagnostic pharmaceuticals such as test kits that use immunochromatography.

[0062] [3. Test kit] Next, an example of a test kit 50 using the analytical carrier 1 will be described. The test kit 50 is also sometimes referred to as a diagnostic kit. As shown in FIG. 2, the test kit 50 includes a test strip 60 having the analytical carrier 1. Specifically, the test strip 60 includes the analytical carrier 1, a specimen supply section 62, and an absorption section 64. The analytical carrier 1 is provided with a determination section 66. The test kit 50 may further include a case (not shown) for storing the test strip 60.

[0063] The specimen supplying section 62 may contain, for example, a labeled antibody that specifically binds to an antigen, which is the object to be analyzed. A specimen collected from a living organism or the like is supplied to the specimen supplying section 62 and mixed with the labeled antibody to form a mixture. The mixture is developed to the determination section 66 by capillary action of the analytical carrier 1, and excess specimen is absorbed by the absorption section 64.

[0064] The determination unit 66 has, for example, a test line and a control line. For example, a capture antibody that specifically binds to the analyte is immobilized on the test line. When the analyte is contained in the sample, a labeled antibody is immobilized on the capture antibody on the test line via the analyte. For example, an antibody that specifically binds to the labeled antibody is immobilized on the control line. When the mixture containing the sample and the labeled antibody is developed up to the control line, the labeled antibody binds to the antibody immobilized on the control line.

[0065] The labeled antibody contains a label containing labeled particles such as colored particles, colloidal gold particles, or fluorescent beads, and an antibody that binds to the label to form a complex and specifically binds to the analyte. Therefore, if there is a location with a high concentration or density of labeled antibody, the fluorescence of that location can be confirmed by the dense label. Therefore, with the test kit 50, a positive result is indicated when both the test line and the control line are visible, and a negative result is indicated when only the control line is visible. The test line and control line can be confirmed visually when colored particles or colloidal gold particles are used, or by a fluorescence detector such as a fluorescence scanner when fluorescent beads are used.

[0066] The fluorescence can be detected by a fluorescence detector such as a fluorescence scanner, which irradiates the analytical support 1 carrying the analyte with excitation light and detects the fluorescence of the fluorescent beads excited by the excitation light.

[0067] The test kit 50 can be used for, for example, infectious disease testing; genetic analysis; pregnancy testing; livestock testing; and allergen testing for food, animals, plants, metals, house dust, etc.

[0068] Examples of the analyte of the test kit 50 include amino acids, peptides, proteins, genes, sugars, lipids, cells, and complexes thereof. More specifically, the analyte may be a peptide such as PCT (procalcitonin); a protein such as urinary albumin; a hormone such as HCG (human chorionic gonadotropin) and LH (luteinizing hormone); HBs antigen, rotavirus antigen, adenovirus antigen, RSV (Respiratory Syndrome) antigen, or the like. Antigens or antibodies for viral infections such as HBs antibody, HCV (Hepatitis C virus) antibody, HIV antibody, EBV antibody, RSV antibody, rubella virus antibody, measles virus antibody, enterovirus antibody, dengue virus antibody, and SARS antibody; antigens or antibodies for bacterial infections such as pneumococcal antigen, mycoplasma antigen, group A hemolytic streptococcus antigen, legionella antigen, mycobacterium tuberculosis antigen, gonorrhea antigen, tetanus antigen, mycoplasma antibody, Helicobacter pylori antibody, and mycobacterium tuberculosis antibody; antigens or antibodies for chlamydia infections such as chlamydia antigen; antigens or antibodies for spirochete infections such as Treponema pallidum antibody; antigens or antibodies for protozoal diseases such as malaria antibody and toxoplasmosis antibody.

[0069] In addition, the analytical carrier 1 can also be used as, for example, a gas or liquid separation membrane; a moisture-absorbing material; a water-absorbing material; an adsorbent material for adsorbing foreign substances such as pollen, particulate matter, bacteria, odor components, and heavy metals; a wiping sheet; a test sheet for chemicals such as concentrated sulfuric acid, urinalysis, and pH testing; a disinfecting and sterilizing material; a reflective material (standard white plate); a separator for batteries and electric double-layer capacitors; a catalyst carrier; a reaction site for synthetic reactions; and a heat-insulating material. Examples of the separation membrane include reverse osmosis membranes, ion exchange membranes, and gas separation membranes. Examples of the adsorbent material include masks, filtration membranes, and filters.

[0070] [4. Manufacturing method of analytical carrier] Next, a method for manufacturing the analytical support 1 according to this embodiment will be described. The method for manufacturing the analytical support 1 according to this embodiment includes a sintering step, an anodizing step, a dissolving step, a laminating step, and a peeling step. Furthermore, the method for manufacturing the analytical support 1 may include a hydration treatment step as necessary. Each step will be described in detail below.

[0071] <Sintering process> The sintering step is a step of sintering a plurality of aluminum metal particles 113 on an aluminum base 105. This step makes it possible to obtain a sintered material 100, as shown in FIG. 3, which includes an aluminum base 105 and a sintered body 110 formed by stacking the aluminum base 105 and sintering the aluminum metal particles 113. While FIG. 3 illustrates the sintered body 110 in which the aluminum metal particles 113 are sintered onto one surface of the aluminum base 105, it is sufficient that the aluminum metal particles 113 are sintered onto at least one surface of the aluminum base 105, and the aluminum metal particles 113 may also be sintered onto both surfaces of the aluminum base 105. That is, the sintered body 110 may have the aluminum metal particles 113 on one surface of the aluminum base 105, or may have the aluminum metal particles 113 on both surfaces of the aluminum base 105.

[0072] The aluminum metal particles 113 contain at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. Hereinafter, at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys will also be simply referred to as aluminum.

[0073] The aluminum content of high-purity aluminum may be 99.95% by mass or more, 99.99% by mass or more, or 99.995% by mass or more. The aluminum content of pure aluminum may be 99.00% by mass or more, 99.50% by mass or more, or 99.80% by mass or more. The aluminum content of pure aluminum is less than 99.95% by mass. High-purity aluminum and pure aluminum may contain elements other than aluminum (Al). The elements other than aluminum contained in pure aluminum may include one or more of silicon (Si), iron (Fe), copper (Cu), manganese (Mn), magnesium (Mg), chromium (Cr), zinc (Zn), titanium (Ti), vanadium (V), gallium (Ga), nickel (Ni), boron (B), zirconium (Zr), etc. The pure aluminum may be an A1000 series alloy specified in JIS H4000.

[0074] The aluminum alloy contains aluminum and elements other than aluminum. The elements other than aluminum contained in the aluminum alloy may include one or more of silicon (Si), iron (Fe), copper (Cu), manganese (Mn), magnesium (Mg), chromium (Cr), zinc (Zn), titanium (Ti), vanadium (V), gallium (Ga), nickel (Ni), boron (B), zirconium (Zr), etc. The total content of the elements other than aluminum contained in the aluminum alloy may be more than 1 mass%. The total content of the elements other than aluminum contained in the aluminum alloy may be 10 mass% or less, or may be 5 mass% or less. The content of each of the elements other than aluminum contained in the aluminum alloy may be 10 mass% or less, or may be 1 mass% or less. The aluminum alloy may be a 1000 series alloy, a 2000 series alloy, a 3000 series alloy, a 4000 series alloy, a 5000 series alloy, a 6000 series alloy, a 7000 series alloy, or an 8000 series alloy as specified in JIS H4000.

[0075] The average particle diameter of the aluminum metal particles 113 is 0.1 μm or more and 20 μm or less. By setting the average particle diameter of the aluminum metal particles 113 to 0.1 μm or more, the strength of the porous body 10 is improved, and breakage during transportation, etc. can be suppressed. By setting the average particle diameter of the aluminum metal particles 113 to 20 μm or less, the flow rate of the analytical carrier 1 can be improved. The average particle diameter of the aluminum metal particles 113 may be 1 μm or more, 3 μm or more, or 5 μm or more. The average particle diameter of the aluminum metal particles 113 may be 18 μm or less, 15 μm or less, or 10 μm or less. The average particle diameter of the aluminum metal particles 113 before sintering is the D50 value obtained by measuring the particle size distribution on a volume basis using a laser diffraction method. The shape of the particles is not particularly limited and may be spherical, polygonal, irregular, scaly, fibrous, or the like.

[0076] The aluminum metal particles 113 can be produced by a known method. The aluminum metal particles 113 can be produced by, for example, an atomization method, a melt spinning method, a rotating disk method, a rotating electrode method, or other rapid solidification methods. Among these, from the viewpoint of industrial productivity, the aluminum metal particles 113 are preferably produced by an atomization method, and more preferably by a gas atomization method. Specifically, the aluminum metal particles 113 are preferably produced by atomizing a molten metal.

[0077] The aluminum base 105 contains at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. The high-purity aluminum, pure aluminum, and aluminum alloys can be those described for the aluminum metal particles 113. The composition of the aluminum contained in the aluminum metal particles 113 and the composition of the aluminum contained in the aluminum base 105 may be the same as or different from each other.

[0078] The thickness of the aluminum base 105 is greater than 0 μm. The thickness of the aluminum base 105 may be 10 μm or more, or 20 μm or more, depending on the application. The thickness of the aluminum base 105 may be, for example, 1 mm or less, 100 μm or less, 10 μm or less, or 1 μm or less.

[0079] The plurality of aluminum metal particles 113 may be disposed on at least one surface of the aluminum substrate 105 and then sintered. On at least one surface of the aluminum substrate 105, aluminum powder containing the plurality of aluminum metal particles 113 may be disposed, or a green compact obtained by compacting the aluminum powder may be disposed, or a liquid composition such as a slurry containing the plurality of aluminum metal particles 113 may be disposed. The liquid composition may be applied to the surface of the aluminum substrate 105 by a known method such as spray coating, brush coating, roller coating, air knife coating, bar coating, spin coating, dipping, or screen printing. The liquid composition may be applied to the desired thickness of the porous body 10, taking into consideration the composition.

[0080] Before disposing the plurality of aluminum metal particles 113 on the surface of the aluminum substrate 105, the surface of the aluminum substrate 105 may be pretreated. The pretreatment may include a step of roughening the surface of the aluminum substrate 105. The pretreatment is not particularly limited and may be cleaning, etching, blasting, or the like.

[0081] The aluminum powder or composition may contain, as necessary, a pore-forming material, a binder, a sintering aid, a surfactant, a solvent, and the like, in addition to the plurality of aluminum metal particles 113. Any of these may be known.

[0082] The content of aluminum metal particles 113 in the composition is preferably 20% by mass or more, more preferably 30% by mass or more, and even more preferably 31% by mass or more. The content of aluminum metal particles 113 in the composition is preferably 70% by mass or less, more preferably 60% by mass or less, and even more preferably 57% by mass or less.

[0083] The pore-forming material is a material that promotes the formation of voids 12 within the sintered body 110. The pore-forming material may be, for example, particles containing a polymeric material. The pore-forming material preferably has low solubility in the solvent described below. The polymeric material may contain a polysaccharide or a resin. The polysaccharide may contain, for example, starch. The resin may contain a polyolefin such as polyethylene or polypropylene.

[0084] The average particle diameter of the pore-forming material is preferably 0.1 μm or more and 20 μm or less. By setting the average particle diameter of the pore-forming material to 0.1 μm or more, the average pore diameter can be easily set to 0.1 μm or more. By setting the average particle diameter of the pore-forming material to 20 μm or less, the average pore diameter can be easily set to 20 μm or less. The average particle diameter of the pore-forming material may be 0.5 μm or more, or may be 1 μm or more. Furthermore, the average particle diameter of the pore-forming material may be 10 μm or less, or may be 8 μm or less. The average particle diameter of the pore-forming material is the D50 value determined by measuring the particle size distribution on a volume basis by laser diffraction.

[0085] The content of the pore-forming material in the composition may be 5% by mass or more, 10% by mass or more, or 12% by mass or more. The content of the pore-forming material in the composition may be 30% by mass or less, 25% by mass or less, or 23% by mass or less. When the content of the pore-forming material is equal to or greater than the lower limit of the above range, the formation of voids 12 in the sintered body 110 is promoted, and the packing fraction of the sintered body 110 is reduced, making it easier to obtain a sintered body 110 having a desired packing fraction and a porous body 10 having a desired porosity. Furthermore, when the content of the pore-forming material is equal to or less than the upper limit of the above range, an excessive decrease in the packing fraction of the sintered body 110 is prevented, making it easier to suppress a decrease in the strength of the porous body 10.

[0086] The binder preferably has high solubility in the solvent described below. The binder may contain, for example, a synthetic resin such as a carboxy-modified polyolefin resin, a vinyl acetate resin, a vinyl chloride resin, a vinyl chloride-vinyl acetate copolymer resin, a vinyl alcohol resin, a butyral resin, a vinyl fluoride resin, an acrylic resin, a polyester resin, a urethane resin, an epoxy resin, a urea resin, a phenolic resin, an acrylonitrile resin, a nitrocellulose resin, paraffin wax, or polyethylene wax, or a natural resin such as wax, tar, glue, urushi, rosin, or beeswax. The content of the binder in the composition may be 0.5% by mass or more, or 1% by mass or more. The content of the binder in the composition may be 30% by mass or less, or 20% by mass or less.

[0087] The solvent may contain an organic solvent such as water, ethanol, toluene, ketones, esters, etc. To volatilize the solvent, the laminate obtained by applying the composition to the aluminum substrate 105 may be dried at a temperature of 20° C. or higher and 300° C. or lower for 1 minute to 30 minutes, if necessary.

[0088] The sintering temperature is not particularly limited, but is preferably 560°C or higher and 660°C or lower. By setting the sintering temperature to 560°C or higher, the strength of the porous body 10 can be improved. By setting the sintering temperature to 660°C or lower, melting of the aluminum metal particles 113 can be suppressed. The sintering temperature may be 570°C or higher, or 580°C or higher. Furthermore, the sintering temperature may be 650°C or lower, or 620°C or lower.

[0089] The sintering time depends on the sintering temperature, etc., but may be, for example, about 5 to 24 hours. The sintering atmosphere is not particularly limited and may be, for example, a vacuum atmosphere, an inert gas atmosphere, an oxidizing gas atmosphere (air), or a reducing gas atmosphere. Among these, the sintering atmosphere is preferably a vacuum atmosphere or a reducing gas atmosphere. Furthermore, the sintering conditions may be any pressure condition of normal pressure, reduced pressure, or increased pressure.

[0090] When the composition contains a pore-forming material, it is preferable to heat the composition at a temperature of 200°C or higher and 500°C or lower before sintering. By heating the composition at 200°C or higher, the pore-forming material is burned slowly, and more uniformly dispersed voids 12 can be formed in the sintered body 110. By heating the composition at 500°C or lower, oxidation of the surfaces of the aluminum metal particles 113 during heating can be suppressed, and the strength of the porous body 10 can be improved. The heating temperature may be 250°C or higher, or 280°C or higher. The heating temperature may be 460°C or lower, or 430°C or lower.

[0091] The heating time is preferably 5 hours or more and 20 hours or less. By setting the heating time to 5 hours or more, more uniformly dispersed voids 12 can be formed in the sintered body 110. By setting the heating time to 20 hours or less, sintering between the aluminum metal particles 113 can be prevented from progressing, and more uniformly dispersed voids 12 can be formed in the sintered body 110. The heating time may be 7 hours or more or 15 hours or less. The sintering atmosphere may be any of a vacuum atmosphere, an inert gas atmosphere, and an oxidizing gas atmosphere. Furthermore, the sintering conditions may be any of atmospheric pressure, reduced pressure, and increased pressure.

[0092] The sintering step may include a first sintered layer forming step for obtaining a first sintered layer and a second sintered layer forming step for obtaining a second sintered layer. In the first sintered layer forming step, a plurality of aluminum metal particles 113 are sintered to obtain the first sintered layer. As described above, the plurality of aluminum metal particles 113 may be arranged on at least one surface of the aluminum base material 105 and then sintered. In the second sintered layer forming step, a laminate in which the plurality of aluminum metal particles 113 are arranged on the surface of the first sintered layer may be sintered. In the first sintered layer forming step and the second sintered layer forming step, at least one of the average particle size, aspect ratio, constituent material, composition of the composition, or type or average particle size of the pore-forming material of the plurality of aluminum metal particles 113 used may be different.

[0093] The filling rate of the sintered body 110 is 10% to 60% by volume. By setting the filling rate of the sintered body 110 to 10% by volume or more, it becomes easier to obtain a porous body 10 having a porosity equal to or lower than the upper limit of the desired range after the anodizing step and the dissolving step. This makes it possible to suppress peeling of the aluminum metal particles 113 from the porous body 10 and improve the strength of the porous body 10. Furthermore, by setting the filling rate of the sintered body 110 to 60% by volume or less, it becomes easier to obtain a porous body 10 having a porosity equal to or higher than the lower limit of the desired range after the anodizing step and the dissolving step. This makes it possible to improve the suction performance of the porous body 10. The filling rate may be 15% by volume or more, or may be 20% by volume or more. Furthermore, the filling rate may be 55% by volume or less, or may be 50% by volume or less. As described in the Examples section below, the filling rate can be obtained by dividing the mass of the sintered body 110 obtained by subtracting the mass of the aluminum base material 105 from the mass of the entire sintered material 100 by the mass of the sintered body 110 when the filling rate is assumed to be 100%.

[0094] <Hydration process> The method for manufacturing the analytical support 1 according to this embodiment may include a hydration step prior to the anodization step. The hydration step is a step for forming a hydrated film on the surface of the aluminum metal particles 113, thereby converting the hydrated film into an oxide film during the subsequent anodization step, thereby efficiently forming an anodized film. The hydration step also has the effect of lifting and cleaning dirt and other contaminants present on the aluminum surface before anodization, thereby preventing uneven appearance when an anodized film is subsequently formed. The hydration step is a step for heat-treating the sintered material 100 with warm water such as boiling water. The warm water may be pure water or an aqueous phosphoric acid solution containing dissolved phosphoric acid. The concentration of the aqueous phosphoric acid solution may be, for example, 0.001 mL / L to 5 mL / L. A phosphoric acid solution of this concentration can be obtained, for example, by adjusting an 85% by mass aqueous phosphoric acid solution with pure water to the desired concentration. The addition of phosphoric acid enhances the surface cleaning effect. When the sintered body 110 is hydrated, a hydrated film of aluminum hydroxide is formed on the surface of the aluminum, and as described above, the hydrated film may be included in the outer shell 14, but the outer shell 14 may also include a hydrated film.

[0095] <Anodizing process> 4, the anodizing process is a process of anodizing the sintered material 100 to form an outer shell 14 including an anodized film on the surface of the aluminum metal particles 113. In addition, in the anodizing process, a coating layer 17, 117 including an anodized film containing aluminum oxide may be formed on the surface of the aluminum base material 105 on which the aluminum metal particles 113 are stacked. In the anodizing process, for example, an anode on which the sintered material 100 is placed and a cathode on which stainless steel (SUS) is placed are immersed in an electrolytic solution to perform electrolysis.

[0096] The electrolyte used in the anodization step may contain at least one selected from the group consisting of citric acid, boric acid, phosphoric acid, sulfuric acid, oxalic acid, and salts thereof. A barrier-type anodic oxide film can be formed by anodizing with an electrolyte containing citric acid, boric acid, phosphoric acid, or a salt thereof. When forming a barrier-type anodic oxide film, it is easier to form an anodic oxide film with a consistent thickness by controlling the voltage, compared to a porous-type anodic oxide film. A porous-type anodic oxide film can be formed by anodizing with an electrolyte containing sulfuric acid, oxalic acid, phosphoric acid, or a salt thereof. When forming a porous-type anodic oxide film, it is possible to form the anodic oxide film at a lower voltage than a barrier-type anodic oxide film, thereby reducing electricity costs. Examples of salts include ammonium salts, sodium salts, potassium salts, and silicates. From the viewpoint of availability, it is preferable that the salt contains at least one of an ammonium salt and a sodium salt.

[0097] The conditions for anodization are not particularly limited. For example, the electrolysis temperature may be 0°C or higher, 10°C or higher, 20°C or higher, 30°C or higher, 40°C or higher, or 50°C or higher. The electrolysis temperature may be 70°C or lower, 60°C or lower, or 50°C or lower. The electrolysis voltage may be 0.1V or higher or 1V or higher, depending on the type of electrolyte. The electrolysis voltage may be 500V or lower or 400V or lower, depending on the type of electrolyte. The electrolysis time may be 0.1 minutes or longer or 1 minute or longer. The electrolysis time may be 60 minutes or shorter or 20 minutes or shorter. Anodization may be performed in a single step or may be performed in multiple separate steps.

[0098] <Dissolution process> As shown in FIG. 5, the dissolving step is a step of dissolving aluminum metal particles 113 surrounded by outer shells 14. The dissolving step can be performed, for example, by immersing the sintered material 100, on which the outer shells 14 have been formed in the anodizing step, in a dissolving solution. In the dissolving step, the high-purity aluminum, pure aluminum, and aluminum alloy contained in the aluminum metal particles 113 can be dissolved inside the outer shells 14 and eluted to the outside of the outer shells 14. This creates cavities 15 inside the outer shells 14. As a result, the outer shells 14 remain as shell-like structures, forming hollow particles 13. In other words, hollow aluminum particles including the outer shells 14 and the cavities 15 surrounded by the outer shells 14 are formed. In the sintered body 110, a continuous series of outer shells 14 is formed on the outer surfaces of adjacent aluminum metal particles 113. Therefore, the anodization process leaves the outer shells 14 derived from the plurality of aluminum metal particles 113 in a continuous form, while the dissolution process dissolves the inside of the aluminum metal particles 113, forming a plurality of connected, continuous cavities 15 inside the outer shells 14. As a result, a skeleton 11 is formed in which the plurality of hollow particles 13 are aggregated.

[0099] The dissolving solution used to dissolve the aluminum metal particles 113 in the dissolving step may contain at least one acid selected from the group consisting of phosphoric acid, sulfuric acid, nitric acid, and salts thereof, or at least one alkali selected from the group consisting of sodium hydroxide and potassium hydroxide. Such a dissolving solution has excellent solubility for the aluminum metal particles 113. The dissolving solution preferably contains, for example, phosphoric acid. The salt contained in the aqueous solution may contain at least one metal salt selected from the group consisting of aluminum, sodium, magnesium, calcium, and zinc.

[0100] When phosphoric acid is used as the dissolving solution, the concentration of phosphoric acid may be, for example, 0.1 g / L to 1000 g / L. The dissolving temperature in the dissolving step may be, for example, 50° C. to 80° C. The dissolving time in the dissolving step may be 1 minute to 60 minutes.

[0101] In the method for producing the analytical support 1, a metal member (aluminum member) 120 is formed by repeating an anodizing step and a dissolving step, in which a porous body 10 is laminated on an aluminum substrate 105. By repeating the anodizing step and the dissolving step, hollow particles 13 can be formed, each having an outer shell 14 including an anodized film containing aluminum oxide and a cavity 15 surrounded by the outer shell 14. FIG. 5 illustrates the metal member 120 in which the porous body 10 is formed on one surface of the aluminum substrate 105. However, it is sufficient that the porous body 10 is formed on at least one surface of the aluminum substrate 105, and the porous body 10 may be formed on both surfaces of the aluminum substrate 105. That is, the metal member 120 may be formed by laminating the porous body 10 on one surface of the aluminum substrate 105, or by forming the porous body 10 on both surfaces of the aluminum substrate 105.

[0102] In the method for manufacturing the analytical support 1, the anodization step and the dissolution step are alternately repeated, and the number of times the anodization step and the dissolution step are repeated may be from two to twenty. The number of times the anodization step and the dissolution step are repeated is not particularly limited, as it is affected by the voltage conditions in the anodization step and the processing time in the dissolution step. However, by repeating the anodization step two or more times, the amount of metallic aluminum remaining in the hollow particles 13 can be reduced, thereby improving the light transmittance of the porous body 10. This makes it possible to provide an analytical support 1 with high visibility. Furthermore, since the amount of residual metallic aluminum is unlikely to be further reduced even if the number of repetitions exceeds 20, work efficiency can be improved by limiting the number of repetitions to twenty or less. The number of repetitions may be, for example, two or more, three or more, five or more, or eight or more. The number of times the anodization step and the dissolution step are repeated may be 15 or less, or 10 or less.

[0103] In the dissolving step, the high-purity aluminum, pure aluminum, and aluminum alloy contained in the aluminum base 105 can be dissolved inside the aluminum base 105 and eluted to the outside of the aluminum base 105. This creates a space inside the aluminum base 105 equivalent to the amount of eluted material. Here, dissolution in the dissolving step proceeds from the aluminum metal particles 113 to a location where the aluminum base 105 and the aluminum metal particles 113 are sintered together and where high-purity aluminum, pure aluminum, and aluminum alloys are present. For this reason, dissolution is more likely to proceed on the side of the aluminum base 10 where the coating layer 17 is present, which is formed on the same side as the porous body 10 and covers the inner surface of the aluminum base 105 while communicating with the porous body 10 side via the communicating holes 18, than on the side of the aluminum base 10 opposite the porous body 10 where the coating layer 117 is present and which is formed to cover the entire outer surface of the aluminum base 105. As the dissolution of the porous body 10 proceeds in this manner, the aluminum substrate 105 on the same side as the porous body 10, which is present near the boundary between the aluminum substrate 105 and the coating layer 17, dissolves, leaving a portion that connects the aluminum substrate 105 on the opposite side of the porous body 10 to the coating layer 17. If the dissolution proceeds too quickly, the aluminum substrate 105 and the coating layer 17 will peel off before the subsequent lamination step, reducing handleability and making it difficult to prepare an analytical carrier 1 in which the porous body 10 is supported on the support 20. If the dissolution proceeds too slowly, it will be difficult to separate the porous body 10 from the aluminum substrate 105 in the subsequent peeling step. For this reason, it is preferable to continue dissolution until the porous body 10 side of the aluminum substrate 105 and the coating layer 17 are partially connected.

[0104] <Lamination process> As shown in FIG. 6 , the lamination step is a step of adhering the porous body 10 of the metal member 120 to the support 20 to form a laminate 130 in which the aluminum substrate 105, the porous body 10, and the support 20 are laminated in this order. In the lamination step, the adhesive layer 22 is adhered to the outer shell 14. By adhering the porous body 10 to the support 20, the porous body 10 can be supported by the support 20. Furthermore, by laminating the aluminum substrate 105, the porous body 10, and the support 20 in this order, the aluminum substrate 105 can be easily peeled off in the peeling step described below. While FIG. 6 illustrates an example of a laminate 130 in which the support 20 is adhered to the porous body 10 formed on one side of the aluminum substrate 105, it is sufficient that the support 20 is adhered to at least one side of the aluminum substrate 105, and the support 20 may also be adhered to both sides of the aluminum substrate 105. That is, the metal member 120 may have a support 20 laminated on a porous body 10 formed on one side of the aluminum base material 105, or may have a support 20 laminated on a porous body 10 formed on both sides of the aluminum base material 105.

[0105] The support 20 before the porous body 10 is adhered is placed on a white reflection standard for which the measuring instrument is calibrated, and the surface of the support 20 to which the porous body 10 is adhered is measured with the measuring instrument. * a * b * L in color space * The value is 75 or more. * By setting the value to 75 or more, it is possible to improve the discrimination ability when water is absorbed. * The value may be 80 or greater, 85 or greater, 90 or greater, or 95 or greater.

[0106] As described above, the support 20 may include a support layer 21 and an adhesive layer 22. The porous body 10 and the support layer 21 may be bonded together with an adhesive. The adhesive may be cured to form the adhesive layer 22. The adhesive may be at least one selected from the group consisting of a solvent-volatile adhesive, a moisture-curing adhesive, a heat-curing adhesive, a curing agent mixed adhesive, an anaerobic-curing adhesive, a UV-curing adhesive, a hot-melt adhesive, a pressure-sensitive adhesive, and a remoisturizing adhesive. The adhesive may contain, for example, an ethylene-vinyl acetate copolymer, and may bond the porous body 10 and the support layer 21 together by heat melting.

[0107] For example, the support 20 may be a single layer of thermoplastic resin, and the porous body 10 may be bonded to the support 20 by applying heat to one side of the support 20 to melt it. Alternatively, the support 20 may not be provided with a support layer 21, and an adhesive may be applied directly to the porous body 10 and cured to form the support 20 consisting of the adhesive layer 22. The first to fourth lamination steps according to the configuration of the support 20 will be described below.

[0108] As shown in FIG. 7 , the first lamination method can be applied to a support 20a in which a support layer 21a made of a thermoplastic resin and an adhesive layer 22a made of a thermoplastic resin having a softening temperature lower than that of the support layer 21a are pre-laminated and integrated. Such a support 20a is commercially available as a laminate film. In the first lamination method, the support 20a is superimposed on the metal member 120 such that the adhesive layers 22a face the outer sides of the metal member 120, respectively, and the support layer 21a faces outward. In this superimposed state, heat is applied to the support 20a from both outer sides of the support layers 21a, sufficient to soften the adhesive layer 22a but not the support layer 21a, thereby softening the adhesive layer 22a and bonding the adhesive layer 22a to the metal member 120. As a result, the metal member 120 and the adhesive layer 22a are bonded to each other on both sides of the metal member 120, and the support layer 21a is provided on the outer side of the adhesive layer 22a, thereby forming a laminate 130a in which the support layer 21a, adhesive layer 22a, metal member 120, adhesive layer 22a, and support layer 21a are laminated in this order. When bonding is performed by applying heat to the adhesive layer 22a, it is preferable to sandwich the overlapped metal member 120 and supports 20a, 20a between at least a pair of rollers, and rotate the rollers to move the metal member 120 and supports 20a, 20a while applying heat and pressure to the metal member 120 and supports 20a, 20a.

[0109] As shown in FIG. 8 , the second lamination method can be applied when the support body 20b is formed by bonding an adhesive layer 22b made of a thermoplastic resin to a support layer 21b that is adhesive to the adhesive layer 22b. In the second lamination method, the adhesive layers 22b, 22b and the support layers 21b, 21b are stacked in this order on both outer sides of the metal member 120. In this stacked state, heat sufficient to soften the adhesive layer 22b is applied from both outer sides where the support layers 21b, 21b are arranged, thereby softening the adhesive layer 22b and bonding the adhesive layer 22b to the metal member 120 and the support layer 21b. As a result, the adhesive layer 22b and the support layer 21b are bonded and integrated to form the support body 20b, and the metal member 120 and the support layer 21b are bonded to each other via the adhesive layer 22b. Furthermore, by bonding the metal member 120 and the adhesive layer 22b on both sides of the metal member 120 and providing the support layer 21b on the outer side of the adhesive layer 22b, it is possible to form a laminate 130b in which the support layer 21b, adhesive layer 22b, metal member 120, adhesive layer 22b, and support layer 21b are laminated in this order. When bonding is performed by applying heat to the adhesive layer 22b, it is preferable to sandwich the laminated metal member 120, adhesive layers 22b, 22b, and support layers 21b, 21b, between at least a pair of rollers, and rotate the rollers to move the metal member 120, adhesive layers 22b, 22b, and support layers 21b, 21b, while applying heat and pressure to the metal member 120, adhesive layers 22b, 22b, and support layers 21b, 21b.

[0110] The third lamination method can be applied when the support 20c consists solely of an adhesive layer 22c made of a thermoplastic resin. In the third lamination method, adhesive layers 22c, 22c, and a release material 25 are laminated in this order on both outer sides of the metal member 120. A suitable release material 25 is a sheet-like material that does not soften when heated and can transfer heat to the adhesive layer 22c without bonding to the adhesive layer 22c, 22c. The release material 25 can be, for example, a paper sheet (release paper). With the support 20c and the release materials 25 laminated together, heat sufficient to soften the adhesive layer 22 is applied from both outer sides where the release materials 25 are located, softening the adhesive layer 22 and bonding the adhesive layer 22 to the metal member 120 in the same manner as in the first and second lamination methods. After bonding, the release material 25 is peeled off from the adhesive layer 22, as shown in FIG. 9 . As a result, the metal member 120 and the adhesive layer 22 are bonded to each other on both sides of the metal member 120, and a laminate 130c can be formed in which the adhesive layer 22, the metal member 120, and the adhesive layer 22 are laminated in this order. When bonding is performed by applying heat to the adhesive layer 22, it is preferable to sandwich the laminated metal member 120, the adhesive layers 22, 22, and the peeling body 25 between at least a pair of rollers, and rotate the rollers to move the metal member 120, the adhesive layers 22, 22, and the peeling body 25 while applying heat and pressure to the metal member 120, the adhesive layers 22, 22, and the peeling body 25.

[0111] The fourth lamination method can be applied when the support body 20d is formed by bonding an adhesive layer 22d made of an adhesive (pressure-sensitive adhesive) to a support layer 21d that is adhesive to the adhesive layer 22d. As shown in Fig. 10, in the fourth lamination method, first, an adhesive is applied to one side of each of the support layers 21d to form the adhesive layers 22d. Next, the support layers 21d with the adhesive layers 22d formed thereon are overlapped on both outer sides of the metal member 120, with the adhesive layers 22d facing the metal member 120. In this overlapped state, a force sufficient to bond the adhesive layer 22d to the metal member 120 and the support layer 21d is applied from both outer sides where the support layers 21d are arranged, thereby bonding the adhesive layer 22d to the metal member 120 and the support layer 21d. As a result, adhesive layer 22d and support layer 21d are bonded and integrated to form support body 20d, and metal member 120b and support layer 21d are bonded via adhesive layer 22d. Furthermore, by bonding metal member 120 and adhesive layer 22d on both sides of metal member 120 and providing support layer 21d on the outer side of adhesive layer 22b, it is possible to form stacked body 130d in which support layer 21d, adhesive layer 22d, metal member 120, adhesive layer 22d, and support layer 21d are stacked in this order. When applying force to the adhesive layer 22b to perform bonding, it is preferable to sandwich the superimposed metal member 120, adhesive layers 22d, 22d, and support layers 21d, 21d between at least a pair of rollers, and rotate the rollers to move the metal member 120, adhesive layers 22d, 22d, and support layers 21d, 21d while applying pressure to the metal member 120, adhesive layers 22d, 22d, and support layers 21d, 21d.

[0112] <Peeling process> As shown in FIG. 11 , the peeling step is a step of dissolving the aluminum substrate 105 and peeling the aluminum substrate 105 from the laminate 130 to form the analytical support 1. Peeling the aluminum substrate 105 from the laminate 130 makes the porous body 10 visible. FIG. 11 illustrates the laminate 130 from which the aluminum substrate 105 and the coating layer 117 have been peeled. However, the analytical support 1 may be formed from at least one surface of the laminate 130 by peeling the aluminum substrate 105, or may be formed from both surfaces of the laminate 130. That is, the laminate 130 subjected to the peeling step may have the support 20 laminated on the porous body 10 formed on one surface of the aluminum substrate 105, or may have the support 20 laminated on the porous body 10 formed on both surfaces of the aluminum substrate 105.

[0113] In the peeling step, a stripping solution may be used to dissolve the aluminum base 105. In the peeling step, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, or 90% or more of the aluminum base 105 may be dissolved.

[0114] In the peeling step, the aluminum substrate 105 may be dissolved with an acid solution or an alkaline solution to separate the coating layer 117 formed on the surface of the aluminum substrate 105 opposite the porous body 10 from the porous body 10. This makes it possible to produce an analytical carrier 1 including the porous body 10 including the coating layer 17 and the support 20. The aluminum substrate 105 may be dissolved, and the coating layer 117 may be naturally peeled off from the porous body 10. Alternatively, the aluminum substrate 105 may be partially dissolved, and the aluminum substrate 105 may be forcibly peeled off manually or mechanically from the laminate 130. Alternatively, the aluminum substrate 105 may be forcibly peeled off manually or mechanically from the laminate 130 after the lamination step without dissolving the aluminum substrate 105.

[0115] The stripping solution used in the stripping step may contain at least one alkali selected from the group consisting of sodium hydroxide and potassium hydroxide. Stripping solutions containing these alkalis have low viscosity and tend to penetrate deep into the porous body 10. Therefore, the use of such a stripping solution can promote the stripping of the aluminum substrate 105. The stripping solution may be an aqueous solution, such as an aqueous solution containing sodium hydroxide.

[0116] The stripping solution used in the stripping step may contain at least one organic acid selected from the group consisting of citric acid and gluconic acid. Stripping solutions containing these organic acids can convert dissolved aluminum into a complex in the stripping solution. Therefore, by using these stripping solutions, it is possible to suppress aluminum precipitation and improve the stability of the stripping solution. The stripping solution may contain the above-mentioned alkali and organic acid. The stripping solution may contain, for example, sodium hydroxide and citric acid.

[0117] The stripping solution used in the stripping step may contain at least one acid selected from the group consisting of phosphoric acid, sulfuric acid, and nitric acid. Stripping solutions containing these acids can also strip the aluminum substrate 105. The stripping solution may contain, for example, phosphoric acid, or may contain phosphoric acid and sulfuric acid.

[0118] The temperature of the stripping solution in the stripping step may be 30°C or higher and 70°C or lower. By setting the temperature of the stripping solution to 30°C or higher, the stripping of the aluminum substrate 105 can be promoted and the time required for the stripping step can be shortened. By setting the temperature of the stripping solution to 70°C or lower, the generation of excessive bubbles can be suppressed. This can suppress damage to the porous body 10 in the stripping step.

[0119] In the stripping step, the aluminum substrate 105 may be stripped in a single step using only one type of stripper. Alternatively, the stripping step may involve stripping the aluminum substrate 105 in multiple steps. When the aluminum substrate 105 is stripped in multiple steps, the same type of stripper may be used in each step, or different types of stripper may be used. When the aluminum substrate 105 is stripped in multiple steps, the stripping conditions, such as the stripping temperature and stripping time, may be the same or different in each step. Alternatively, after treating the aluminum substrate 105 with the stripper, it may be subjected to treatments such as neutralization with a solution containing phosphoric acid, washing with water, and drying.

[0120] <Action and effect> As described above, the method for manufacturing an analytical support 1 according to this embodiment is a method for manufacturing an analytical support 1 including a porous body 10 and a support 20 that supports the porous body 10 on one side. The porous body 10 includes a skeleton 11 formed by an aggregation of a plurality of hollow particles 13, and a plurality of voids 12 surrounded by the skeleton 11. The hollow particles 13 have an outer shell 14 including an anodized coating containing aluminum oxide, and a cavity 15 surrounded by the outer shell 14. The skeleton 11 is formed by the continuous outer shells 14 of the plurality of hollow particles 13. The support 20 also includes an adhesive layer 22 that adheres to the porous body 10. The manufacturing method includes a sintering step of sintering a plurality of aluminum metal particles 113 on an aluminum substrate 105 to obtain a sintered material 100 including the aluminum substrate 105 and a sintered body 110 in which the aluminum metal particles 113 are stacked on the aluminum substrate 105 and sintered. The manufacturing method includes an anodizing step of anodizing the sintered material 100 to form shells 14 including an anodized film on the surfaces of the aluminum metal particles 113. The manufacturing method also includes a dissolving step of dissolving the aluminum metal particles 113 surrounded by the shells 14. In the manufacturing method, the anodizing step and the dissolving step are repeated to form a metal member 120 in which a porous body 10 is laminated on an aluminum substrate 105. The manufacturing method also includes a laminating step of adhering the porous body 10 of the metal member 120 to a support 20 to form a laminate 130 in which the aluminum substrate 105, the porous body 10, and the support 20 are laminated in this order. In this laminating step, the porous body 10 is adhered to an adhesive layer 22 to form a laminate 130 in which the aluminum substrate 105, the porous body 10, and the adhesive layer 22 are laminated in this order. The manufacturing method also includes a peeling step of dissolving the aluminum substrate 105 to peel the aluminum substrate 105 from the laminate 130. The aluminum metal particles 113 contain at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. The aluminum base 105 contains at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. The average particle diameter of the plurality of aluminum metal particles 113 is 0.1 μm or more and 20 μm or less.The packing rate of the sintered body 110 is 10% by volume or more and 60% by volume or less. According to such a manufacturing method, the above-mentioned analytical support 1 can be manufactured. [Example]

[0121] Hereinafter, the present embodiment will be described in more detail with reference to examples and comparative examples, but the present embodiment is not limited to these.

[0122] [Example 1] (Slurry preparation) First, 24 parts by mass of aluminum metal particles, 26 parts by mass of a pore-forming material, and 1 part by mass of a binder were uniformly dispersed in 49 parts by mass of a solvent to prepare a slurry. The aluminum metal particles (AHZL58FN manufactured by Toyo Aluminum K.K.) were pure aluminum (JIS A1080) with a purity of 99.80% by mass or more. The aluminum metal particles were approximately spherical and had an average particle diameter of 3 μm. Starch (Nikka Co., Ltd., Nikka ...) with an average particle diameter of 7 μm was used as the pore-forming material. Ethyl cellulose resin was used as the binder. Butyl acetate was used as the solvent.

[0123] (sintering) The slurry was applied to one side of a 30 μm thick aluminum substrate (aluminum base material) using a Comma Coater (registered trademark) manufactured by Hirano Tecseed Co., Ltd., so that the thickness of the sintered body would be 50 μm. The slurry was dried at 100°C for 1.5 minutes, then heated at 350°C for 5 hours in an air atmosphere, and further sintered at 620°C to 640°C for 10 hours in an argon gas atmosphere. In this way, a sintered material was produced in which a sintered body was provided on an aluminum substrate.

[0124] (hydration treatment) Next, the sintered material was washed with a 5 g / L oxalic acid solution at 50°C for 3 minutes. After that, the sintered material was immersed in an aqueous solution prepared by adjusting the concentration of 85% phosphoric acid solution with pure water to 0.5 mL / L for 3 minutes at 85°C for hydration treatment. Next, the hydrated sintered material was washed with pure water at room temperature (30°C) for 1 minute.

[0125] (First anodization) The hydrated sintered material was subjected to a first anodization to form an outer shell containing aluminum oxide on the surface of the aluminum metal particles. Specifically, the sintered material placed on the anode and stainless steel (SUS) placed on the cathode were immersed in an electrolyte at 50°C prepared with pure water to contain 1 g / L of 98% citric acid and 0.1 g / L of 95% triammonium citrate. Then, a current of 50 mA / cm was applied until the voltage reached 450 V. 2 The anodized sintered material was then washed with pure water for 0.5 minutes.

[0126] (First dissolution treatment) The first anodized sintered material was immersed in an aqueous solution prepared by adjusting the concentration of 85% phosphoric acid to 500 mL / L (718 g / L) with pure water at 70°C for 1 to 5 minutes to further dissolve the aluminum metal. The sintered material that had undergone the first dissolution treatment was then washed with pure water for 1 minute.

[0127] (Second anodization) The sintered material that had undergone the first dissolution treatment was subjected to a second anodization, and an outer shell containing aluminum oxide was further formed on the surface of the aluminum metal particles. Specifically, the sintered material placed on the anode and stainless steel (SUS) placed on the cathode were immersed in an electrolyte at 50°C prepared with pure water so that 98% citric acid was 1 g / L and 95% triammonium citrate was 0.1 g / L. Then, a current of 50 mA / cm was applied until the voltage reached 50 V. 2 The sintered material was anodized by applying a current of 50 V and holding the voltage for 3 minutes. The anodized sintered material was then washed with pure water for 0.5 minutes.

[0128] (Second dissolution treatment) The sintered material that had undergone the second anodization was subjected to a second dissolution treatment in which the aluminum metal was further dissolved by immersing it in an aqueous solution prepared by mixing 85% phosphoric acid with pure water at 500 mL / L (718 g / L) at 70°C for 1 to 5 minutes. The sintered material that had undergone the second dissolution treatment was then washed with pure water for 1 minute.

[0129] (Third anodization) The sintered material that had undergone the second dissolution treatment was subjected to a third anodization to further form an outer shell containing aluminum oxide on the surface of the aluminum metal particles. Specifically, the sintered material placed on the anode and stainless steel (SUS) placed on the cathode were immersed in an electrolyte at 50°C prepared with pure water to contain 1 g / L of 98% citric acid and 0.1 g / L of 95% triammonium citrate. Then, a current of 50 mA / cm was applied until the voltage reached 50 V. 2 The anodized sintered material was washed with pure water for 0.5 minutes.

[0130] (Third dissolution treatment) The sintered material that had undergone the second anodization was subjected to a third dissolution treatment in which the aluminum metal was further dissolved by immersing it in an aqueous solution prepared by mixing 85% phosphoric acid with pure water at 30 mL / L (43 g / L) at 70°C for 5 to 30 minutes. The sintered material that had undergone the third dissolution treatment was then washed with pure water for 1 minute.

[0131] (Drying process) The sintered material that had been subjected to the third melting treatment was dried at 200° C. for 2 minutes to produce the aluminum member according to this example.

[0132] (Lamination processing) The lamination process was carried out by lamination method 1. Lamination method 1 is as follows.

[0133] (Lamination method 1) A transparent laminate film was used as a support. The aluminum member and the laminate film were laminated on both sides of the aluminum member prepared as described above, with the laminate film serving as the support layer. A Fellowes laminate film (PET / LDPE / EVA, 100 μm thick, GLOSS) was used as the laminate film. A PET film was used as the adhesive layer, and an LDPE / EVA film was used as the support layer. A Fellowes Proteus A3 laminator was used, and the aluminum member was laminated onto the laminate film at a lamination speed of approximately 1.4 cm / s so that the lamination temperature was 90°C. The laminate consisting of the aluminum member and the laminate film was cut to a width of 25 mm. Lamination method 1 corresponds to the first lamination method described above.

[0134] (peeling treatment) The peeling treatment was carried out under peeling conditions 2. The peeling conditions 2 are as follows.

[0135] (Peeling condition 2) The 25 mm wide laminate obtained as described above was immersed for 30 minutes at 40°C in an aqueous solution prepared with pure water to give 450 g / L of sodium hydroxide and 10 g / L of citric acid. This treatment allowed the aluminum substrate to be peeled off and removed from the laminate. The laminate was then neutralized by immersion for 5 minutes at 70°C in an aqueous solution prepared with pure water to give 40 mL / L of 85% phosphoric acid.

[0136] (Drying process) The laminate from which the aluminum substrate had been removed was washed with pure water for 1 minute and dried at 50° C. for 10 minutes to prepare an analytical carrier according to this example.

[0137] [Examples 2 to 29] An analytical carrier was prepared in the same manner as in Example 1, except for the conditions shown in Tables 1 to 7. The lamination methods 2 to 4 shown in Table 7 are as follows. The peeling conditions 1 and 3 shown in Table 7 are as follows. The aluminum metal particles used were as follows.

[0138] (Lamination method 2) Using the adhesive layer and support layer shown in Table 1, the adhesive layer and support layer were superimposed on both sides of the aluminum member prepared as described above, and the adhesive layer was laminated with the aluminum member and support layer to produce a laminate in which supports consisting of the adhesive layer and support layer were laminated on both sides of the aluminum member. Using the same laminator as in Example 1, lamination was performed at a lamination speed of approximately 0.7 cm / s so that the lamination temperature was 140°C. The laminate was cut to a width of 25 mm. Lamination method 2 corresponds to the second lamination method described above.

[0139] (Lamination method 3) Using the adhesive layer shown in Table 1, the adhesive layer and release paper were laminated on both sides of the aluminum member prepared as described above, and the adhesive layer and aluminum member were then laminated. The release paper was then peeled off to produce a laminate in which supports made of adhesive layers were laminated on both sides of the aluminum member. Using the same laminator as in Example 1, lamination was performed at a lamination speed of approximately 0.7 cm / s so that the lamination temperature was 140°C. The laminate was cut to a width of 25 mm. Lamination method 3 corresponds to the third lamination method described above.

[0140] (Lamination method 4) Using the adhesive layer and support layer listed in Table 1, adhesive was applied to both sides of the support layer to form adhesive layers, and then the support layer with the adhesive layer formed thereon was placed on both outer sides of the aluminum member prepared as described above, with the adhesive layer facing the aluminum member, and the adhesive layer was then bonded to the aluminum member and support layer to produce a laminate in which a support consisting of an adhesive layer and a support layer was laminated on both sides of the aluminum member. The adhesive used was Aron Alpha EXTRA Impact Resistant (cyanoacrylate adhesive) manufactured by Toagosei Co., Ltd. The laminate was cut to a width of 25 mm. Lamination method 4 corresponds to the fourth lamination method described above.

[0141] (Peeling condition 1) The 25 mm wide laminate obtained as described above was immersed for 3 minutes at 70°C in an aqueous solution prepared with pure water to give 300 mL / L of 98% sulfuric acid and 300 mL / L of 85% phosphoric acid. Thereafter, the 25 mm wide laminate was immersed for 5 minutes at 70°C in an aqueous solution prepared with pure water to give 500 mL / L of 85% phosphoric acid. Through these treatments, the aluminum substrate was removed from the laminate.

[0142] (Peeling condition 3) The 25 mm wide laminate obtained as described above was removed by manually holding the ends of the analytical carrier portion and the aluminum substrate and pulling them in opposite directions normal to the laminate at a speed of 5 mm / s.

[0143] (aluminum metal particles) Average particle size 1.8 μm: AHU091 manufactured by Toyo Aluminum Co., Ltd. Average particle size 5 μm: AHZL58CN manufactured by Toyo Aluminum Co., Ltd. Average particle size 9 μm: AHZL560F manufactured by Toyo Aluminum Co., Ltd. Average particle size 15 μm: AHZL530C manufactured by Toyo Aluminum Co., Ltd.

[0144] [Comparative Example 1] Analytical carriers were prepared in the same manner as in Example 1 under the conditions shown in Tables 1 to 7, except that the aluminum members that had been subjected to the drying treatment were not subjected to the lamination treatment (lamination treatment) and subsequent treatments.

[0145] [Reference example] Cytiva nitrocellulose membrane AE99 was used as the analytical support.

[0146] [evaluation] The analytical carriers of the respective examples were evaluated as follows.

[0147] (Filling rate) The packing ratio of the sintered body was obtained by measuring the sintered material before hydration treatment according to the following procedure. 100cm projected area from sintered material 2 The measurement sample was then cut out. Measurements and calculations were then carried out on the measurement sample. (In this specification, the projected area refers to the horizontal projected area of ​​the sintered material or sintered body when viewed in a plan view in the same direction as the thickness direction.) The mass Ma (g) of the entire sintered material, which is the combination of the sintered body and the aluminum base material, was measured. The thickness of the entire sintered body, Wa (cm), was measured with a micrometer. The thickness Wb (cm) of the aluminum substrate was measured with a micrometer. The mass of the aluminum substrate, Mb (g), was calculated as Mb = density of aluminum substrate × Wb × 100. In this example, the density of the aluminum base material and the sintered body portion made of aluminum material is 2.7 g / cm 3 was calculated. The mass of the sintered body, Mc (g), was calculated as Mc = Ma - Mb. The mass Md (g) of the sintered part when the filling rate is assumed to be 100% was calculated as follows: Md = density of material of sintered part × (Wa - Wb) × projected area of ​​sintered part = 2.7 × (Wa - Wb) × 100. The packing ratio of the sintered body contained in the measurement sample was calculated as packing ratio (%) = (Mc / Md) × 100.

[0148] (Porosity of porous body) The porosity of the porous body was obtained by dividing the cumulative pore volume of the porous body by the volume of the porous body. The cumulative pore volume of the porous body was obtained by integrating the pore volumes of pores having a pore diameter of 0.1 μm or more and 100 μm or less, which were measured by mercury intrusion porosimetry.

[0149] (Cross-section observation) The analytical support was immersed in liquid nitrogen to freeze it, and then the frozen analytical support was bent and broken. Platinum was vapor-deposited on the fracture surface to a thickness of approximately 5 nm, thereby obtaining a sample for cross-sectional observation. A cross-section of this observation sample in a plane perpendicular to the adhesive interface between the porous body and the adhesive layer was observed using a Carl Zeiss ULTRA Plus scanning electron microscope to obtain a cross-sectional observation photograph (cross-sectional SEM image). The cross-sectional observation photograph of Example 5 is shown in Figure 12. In Figure 12, reference numeral 10 denotes the porous body, reference numeral 20 denotes the support, and reference numeral 80 denotes fragments of the porous body 10 that broke and scattered on the support when broken.

[0150] (Outer shell thickness) The shell thickness was measured from cross-sectional SEM images obtained by the cross-sectional observation method described above. The shell thickness of 10 hollow particles was measured and the average value was calculated to obtain the shell thickness.

[0151] (Thickness of porous body) The thickness of the porous body was measured from a cross-sectional SEM image obtained by the above-mentioned cross-sectional observation method. The thickness of the porous body was measured as the thickness at the position where the thickness in the thickness direction of the material constituting the porous body was greatest within a 50 μm wide range in the direction parallel to the adhesive interface between the porous body and the adhesive layer. The thickness of the adhesive layer was measured at five points in five different ranges and the average value was calculated to obtain the thickness of the porous body.

[0152] (adhesive layer thickness) The thickness of the adhesive layer was measured from cross-sectional SEM images obtained by the cross-sectional observation method described above. The thickness of the adhesive layer was measured as the thickness at the position where the thickness in the thickness direction of the material constituting the adhesive layer was greatest within a 50 μm wide area parallel to the adhesive interface between the porous body and the adhesive layer. The thickness of the adhesive layer was obtained by measuring the thickness of the adhesive layer at five points in five different areas and calculating the average value.

[0153] (thickness of adhesive joint) The thickness of the adhesive joint was measured from a cross-sectional SEM image obtained by observation using the cross-sectional observation method described above. Within a 50 μm-wide area parallel to the adhesive interface between the porous body and the adhesive layer, a line was drawn that passed through the part of the porous body closest to the adhesive layer (the bottom) and was parallel to the adhesive interface between the porous body and the adhesive layer, and a line was drawn that passed through the part of the adhesive layer closest to the porous body (the top) and was parallel to the adhesive interface between the porous body 10 and the adhesive layer 22. The distance between these two lines was measured as the thickness of the adhesive joint 23. The thickness of the adhesive joint was measured at five different points in five different areas, and the average value was calculated to obtain the thickness of the adhesive joint.

[0154] (Surface observation) A sample for surface observation was obtained by depositing platinum on the surface of the analytical carrier so that the platinum film thickness was approximately 5 nm. The porous body side of this sample for observation, i.e., the surface of the coating layer, was observed using a scanning electron microscope ULTRA plus manufactured by Carl Zeiss Co., Ltd., to obtain a surface observation photograph (surface SEM image). A cross-sectional observation photograph of Example 5 is shown in Figure 13.

[0155] (Cumulative pore surface area of ​​porous body) The cumulative pore surface area of ​​the porous body was obtained by converting the pore volume of pores having a pore diameter of 0.1 μm or more and 100 μm or less, measured by mercury intrusion porosimetry, into a surface area for each particle diameter and integrating the converted volume. A graph showing the relationship between pore diameter and differential pore surface area for Example 5 and the Reference Example is shown in Figure 14. A graph showing the relationship between pore diameter and cumulative pore surface area for Example 5 and the Reference Example is shown in Figure 15.

[0156] (Average pore size of porous body (4V / A)) The average pore diameter (4V / A) of the porous body was calculated using the following formula. Average pore diameter of porous body = 4 × (cumulative pore volume of porous body) / (cumulative pore surface area of ​​porous body) The cumulative pore volume of the porous body and the cumulative pore surface area of ​​the porous body were obtained as described above.

[0157] (cumulative pore surface area ratio) The ratio of the cumulative pore surface area of ​​pores having a diameter of 1 μm or more and less than 1 μm to the cumulative pore surface area of ​​pores having a diameter of 1 μm or more and less than 10 μm was calculated. As shown in FIG. 15, the cumulative pore surface area of ​​pores having a diameter of 1 μm or more and less than 10 μm was obtained by integrating the pore surface areas of pores having a diameter of 1 μm or more and less than 10 μm. Similarly, the cumulative pore surface area of ​​pores having a diameter of 0.1 μm or more and less than 1 μm was obtained by integrating the pore surface areas of pores having a diameter of 0.1 μm or more and less than 1 μm. The cumulative pore surface area of ​​the porous body was determined by mercury intrusion porosimetry as described above.

[0158] (Ratio of metallic aluminum to components constituting porous body containing aluminum element) The metallic aluminum content relative to the components constituting the porous body containing aluminum element contained in the porous body was measured as follows, and the ratio of metallic aluminum to the components constituting the porous body containing aluminum element was calculated. 1. The analytical carrier was rubbed with a stainless steel spatula to scrape off the porous portion. 2. 2.0 g was weighed out from the scraped sample. 3. The metallic aluminum content (mass%) of the weighed sample relative to the components constituting the porous body containing the aluminum element was measured in accordance with the metallic aluminum decomposition and separation ICP atomic emission spectroscopic analysis method of JIS G2404:2022. 4. Using the following formula, the ratio of metallic aluminum to the components constituting the porous body containing aluminum element was calculated from the metallic aluminum content (mass%) to the components constituting the porous body containing aluminum element. The ratio of metallic aluminum to the components constituting the porous body containing aluminum element = (metallic aluminum content relative to the components constituting the porous body containing aluminum element) / (100 - (metallic aluminum content relative to the components constituting the porous body containing aluminum element))

[0159] (arithmetic mean roughness Sa) The arithmetic mean roughness Sa of the porous body side surface of the analytical carriers in the examples and comparative examples was measured in accordance with ISO 25178. The arithmetic mean roughness Sa was measured using a Bruker AXS 3D white light interference microscope, ContourGT-I, under conditions of a measurement range of 60 μm × 79 μm, a 115x objective lens, and a 1x internal lens. For the analytical carriers in Examples 1 to 29, the surface of the porous body side supported on the support of the analytical carrier was observed. For Comparative Example 1, the surface of the porous body side supported on the aluminum substrate was observed. For Reference Example 1, the surface of the nitrocellulose membrane supported on the backing sheet was observed. For each sample, a 60 μm × 60 μm image was acquired, and the surface roughness Sa (μm) was calculated. The surface roughness Sa was obtained by calculating the average value of the images from a total of five fields of view.

[0160] (flow rate) The flow rate was measured as follows: First, the analytical carrier was immersed in pure water so that the plane of the analytical carrier was perpendicular to the liquid surface. Then, the time it took for the water to be drawn up by capillary action to a height of 4 cm from the liquid surface after immersion was measured and evaluated as the flow rate.

[0161] (L value when dry) The analytical carrier with the dried porous material is placed on a white reflection standard with which the measuring instrument is calibrated, and the L measured with the measuring instrument is the surface of the porous material on the analytical carrier. * a * b * L in color space * The value was measured. * The values ​​were measured using a 45° circular illumination and vertical light receiving color difference meter (CR-331C color difference meter manufactured by Konica Minolta Japan, Inc.) conforming to JIS Z8722. The white reflection standard used was the MINOLTA white calibration plate CR-A46 (Y: 92.7, x: 0.3129, y: 0.3189).

[0162] (L when absorbing water * value) The analytical carrier with absorbed water in the porous body is placed on a white reflection standard with which the measuring instrument is calibrated, and the L when the porous body surface of the analytical carrier is measured with the measuring instrument. * a * b * L in color space * The values ​​were measured. Specifically, an analytical carrier cut into 3 cm x 3 cm pieces was placed on the bottom of a petri dish filled with pure water to a depth of 5 mm, with the thickness direction perpendicular to the water surface. When the water had been absorbed up to the top of the porous body of the analytical carrier, the analytical carrier was removed from the petri dish and placed on a white reflective standard with the porous body surface of the analytical carrier facing upward. With the porous body of the analytical carrier still absorbing pure water, the surface on which the porous body of the analytical carrier was placed was measured in the same manner as above using a color difference meter (CR-331c manufactured by Konica Minolta).

[0163] (L of support * value) The support before adhering the porous body is placed on a white reflection standard to which the measuring instrument is calibrated, and the L measured with the measuring instrument is the surface of the support where the porous body is to be adhered. * a * b * L in color space * The support was measured in the same manner as above using a color difference meter (CR-331c manufactured by Konica Minolta).

[0164] (bending test) As shown in FIG. 16, 1 mm diameter holes 210 were drilled 5 mm from both ends of an analytical carrier 1 cut to a size of 10 mm x 100 mm. A nylon thread 220 with a diameter of 0.165 mm was threaded through the hole 210, and 10 g E-2 class weights 230 were attached to both ends of the analytical carrier 1. The analytical carrier 1 was placed on a stainless steel rod 240 with a diameter of 2 mm and a length of 5 cm, so that the porous body was in contact with the stainless steel rod 240 at the longitudinal center of the analytical carrier 1. As shown in FIG. 17, the stainless steel rod 240 was slowly lifted until the weights 230 at both ends of the analytical carrier 1 floated, and then held for 10 seconds, after which the stainless steel rod 240 was returned to its original position. The surface of the porous body 10 was then visually observed and evaluated. If no wrinkles remained on the surface of the porous body and no peeling of the porous body occurred, the evaluation was "excellent." If wrinkles remained but no peeling of the porous body occurred, the evaluation was "fair." If wrinkles remained and the porous body peeled, the evaluation was "poor." It was evaluated that wrinkles remained when wrinkles of a size on the order of millimeters or larger that could be visually confirmed were observed. Furthermore, it was evaluated that peeling of the porous body 10 occurred when the porous body 10 and the support 20 separated within the surface of the analytical carrier 1, and the minimum width of the separated portion was 2 mm or more and the maximum length was 5 mm or more.

[0165] (colloidal gold test) SARS-CoV-2 nucleoprotein recombinant was diluted with pseudoserum to concentrations of 0 ng / mL to 100 ng / mL, and 100 μL of each solution was dropped onto the sample pad 340 of the test strip (see Figure 18). The analytical carrier was left to stand for 20 minutes, and the absorbance of the test line and control line was measured using an immunochromatography reader (C10066-10, Hamamatsu Photonics). The results are shown in Tables 9 and 10 and Figure 19. The test strip was prepared as follows.

[0166] <Preparation of test strips> 18, an antibody-immobilized membrane 310, an absorption pad 320, a conjugate pad 330, and a sample pad 340 were attached to a backing sheet in this order, and then cut to a width of 5 mm to prepare a test strip 300. The following reagents and materials were prepared to prepare the test strip 300.

[0167] (1) Preparation of reagents and materials ·Antibody C706:Rabbit Monoclonal anti-SARS-CoV-2 Nucleoprotein C706, manufactured by HyTest ·Antibody C524:Rabbit Monoclonal anti-SARS-CoV-2 Nucleoprotein C524, manufactured by HyTest Anti-rabbit antibody: Goat anti-rabbit IgG h+l Affinity Purified A120 201A, manufactured by HyTest 5mM-PB (pH 7.0): 3mM disodium hydrogen phosphate, 2mM sodium dihydrogen phosphate dodecahydrate 1x PBS: Sodium chloride 8g / L, sodium dihydrogen phosphate dodecahydrate 2.9g / L, potassium chloride 0.2g / L, potassium dihydrogen phosphate 0.2g / L Potassium dihydrogen phosphate: Fujifilm Wako Pure Chemical Industries, #169-04245 Sodium dihydrogen phosphate dodecahydrate: Fujifilm Wako Pure Chemical Industries, Ltd., #193-02845 Sodium chloride: Fujifilm Wako Pure Chemicals, #191-01665 Colloidal Gold Solution: Colloidal Gold Solution-SC, particle size 40nm, Tanaka Kikinzoku PEG: Polyethylene Glycol 20000, Fujifilm Wako Pure Chemical BSA (Albumin, from Bovine Serum, Globulin Free-HG): Fujifilm Wako Pure Chemical Storage buffer: 1% BSA, 0.1% sodium azide, 0.05% PEG, 20 mM Tris-HCl (pH 8.2), 150 mM NaCl Sodium azide: Fujifilm Wako Pure Chemical ·Simulated serum (components: 1×PBS, BSA2%, Tween20 0.05%) Antibody solution for test line: Use 0.5 mL of Ultracel 30K to replace antibody C524 with 5 mM PB, then adjust the final solution volume to 100 μL with 5 mM PB to a concentration of 1 mg / mL. Antibody solution for control line: Use 0.5 mL of Ultracel 30K anti-rabbit antibody, replace with 5 mM PB, and adjust to 0.5 mg / mL with 5 mM PB to a final volume of 100 μL. Blocking buffer: 50 mM boric acid was adjusted to pH 8.5 by adding 50 mM borax little by little, and then 2% casein was added. Washing buffer: 1% hydrochloric acid was added little by little to 50 mM Tris to adjust the pH to 7.5, and then 0.5% sucrose and 0.05% sodium cholate were added. Absorbent Pads: Cellulose Fiber Sample Pads 20 x 300 mm, 100 packs (Merck Millipore, CFSP203000) Backing sheet: Pre-cut backing sheet (Nippon Engineering, 34042 / 11GL-56338) Sample pad: Glassfiber diagnostic pad

[0168] (2) Preparation of antibody-immobilized membrane The antibody solution for the test line was drawn up into a 25 μL syringe (HAMILTON, 702SNR) equipped with a polyethylene capillary tube at its tip. The tip of the capillary tube was lightly brought into contact with the membrane, with the membrane surface and capillary tube at a 45° angle. The antibody solution was dispensed at a rate of 2.5 μL / min, while the test line was swept at a sweep speed of 24 mm / min. The test line was swept 7 mm from the bottom end of the membrane (18 mm from the top end).

[0169] The antibody solution for the control line was drawn up into a 25 μL syringe (HAMILTON, 702SNR) with a polyethylene capillary tube attached to the tip, different from the above. The tip of the capillary tube was lightly touched to the membrane so that the angle between the membrane surface and the capillary tube was 45°, and the antibody solution was dispensed at a rate of 2.5 μL / min while sweeping the control line at a sweep rate of 24 mm / min. The control line was swept 12 mm from the bottom end of the membrane (13 mm from the top end).

[0170] The membrane with the test and control lines swabbed was dried in a thermostatic chamber at 50°C for 1 minute. The membrane, dried as described above, was placed in a Petri dish filled with blocking buffer to a height of 2 mm, with the test line facing downward relative to the control line, and left to stand for 2 minutes. The membrane was then submerged in blocking buffer and left to stand for 3 minutes, after which the membrane was lifted out and excess blocking buffer was shaken off. The membrane was then submerged in washing buffer and left to stand for 10 minutes, after which the membrane was lifted out and the excess washing buffer was absorbed with paper, and dried at 50°C for 10 minutes.

[0171] (3) Preparation of conjugate pad Antibody C706 was prepared at 50 μg / mL using 0.5 mL of Ultracel 30K. After substituting 5 mM PB, ultrapure water was added to a final volume of 100 μL. 900 μL of gold colloid solution was added to a 2.0 mL tube containing 100 μL of 50 mM potassium dihydrogen phosphate (pH 8.0) and stirred. 100 μL of the 50 μg / mL antibody C706 solution prepared as described above was added to the solution with stirring and allowed to stand at room temperature for 10 minutes. 55 μL of 1% PEG was added to the solution and gently stirred. 110 μL of 10% BSA (pH 9.0) was added to the solution and gently stirred. The solution was then centrifuged at 8000 G for 15 minutes at 4°C. The supernatant was removed, leaving approximately 100 μL, and dispersed using an ultrasonic cleaner. 2 mL of colloidal gold storage buffer was added to this dispersion, followed by centrifugation at 8000 G for 15 minutes at 4°C. After centrifugation, all but 100 μL of the supernatant was removed and dispersed using an ultrasonic cleaner. A 1.5 μL aliquot of the dispersed sensitized colloidal gold solution was measured for OD520. The OD520 measurement was converted and adjusted to 6.0 with colloidal gold storage buffer to produce antibody C706-sensitized colloidal gold solution. In a 2 mL tube, 420 μL of antibody C706-sensitized colloidal gold solution, 420 μL of ultrapure water, and 840 μL of coating buffer were mixed. The entire mixture was pipetted evenly onto a glassfiber diagnostic pad (10 mm x 300 mm) and then placed in a desiccator for drying under reduced pressure for at least one day.

[0172] [Table 1]

[0173] [Table 2]

[0174] [Table 3]

[0175] [Table 4]

[0176] [Table 5]

[0177] [Table 6]

[0178] [Table 7]

[0179] [Table 8]

[0180] [Table 9]

[0181] [Table 10]

[0182] [Consider] As shown in Table 8, the analytical carriers according to Examples 1 to 25, 28, and 29 were L * It has a support that has a value of 80 or more. Therefore, the L * On the other hand, in the analytical carrier according to Comparative Example 1, the porous body was laminated on the support, and the aluminum substrate was not peeled off from the porous body. * The value is below 80. Therefore, the dry L * Although the value was over 80, the L * The analytical carriers according to Examples 26 and 27 were L *The support has a value below 80. Therefore, the dry L * Although the value was over 80, the L * The value was below 80.

[0183] Furthermore, as shown in Table 8, the analytical carriers according to Examples 1 to 29 were provided with a support having an adhesive layer, and therefore the bending test results were favorable, and no peeling of the porous body occurred. On the other hand, the analytical carrier according to Comparative Example 1 was not provided with a support having an adhesive layer, and the porous body and the substrate were integrated, so the bending test results were unfavorable, and peeling of the porous body occurred. These results demonstrate that the analytical carriers according to Examples 1 to 29 can prevent the porous body from peeling from the support even when subjected to bending deformation. Furthermore, the analytical carriers according to Examples 1 to 20 and 24 to 29 did not cause peeling of the porous body during the bending test, and no wrinkles remained. These results demonstrate that the thickness of the outer shell was within the desired range, the thickness of the porous body was within the desired range, and the support layer was made of a material that is resistant to plastic deformation, thereby preventing wrinkles from remaining.

[0184] As shown in Tables 9 and 10, the analytical carriers according to Examples 1 to 29 had stronger signals at the test line and control line than the reference example. Here, as shown in Table 3 and FIG. 15, the cumulative pore surface area ratios for the examples were 0.1 or more and 10 or less. Meanwhile, the cumulative pore surface area ratio for the analytical carrier according to the reference example was 0.098. These results demonstrate that the analytical carriers according to the examples have more pores with pore diameters of 0.1 μm or more and less than 1 μm than the analytical carrier according to the reference example. It is believed that the analytical carriers according to the examples had stronger signals at the test line and control line because of the increased amount of antibody bound to the porous body and the increased amount of gold colloid adsorbed to the antibody.

[0185] 12, a cross-sectional SEM image of the analytical carrier 1 of Example 5 confirmed a structure in which the porous body 10 was supported by a support 20 made of EVA / LDPE / PET. It was confirmed that an adhesive layer 22 made of EVA was adhered to the outer shell 14 of the porous body 10. It was confirmed that a coating layer 17 was provided on the surface of the porous body 10 opposite the support 20, and that the coating layer 17 separated the inside and outside of the porous body 10, where the skeleton 11 and voids 12 exist.

[0186] 13, it was confirmed that a coating layer 17 was provided on the surface of the porous body 10. It was confirmed that the hollow particles 13 and outer shells 14 inside the porous body 10 were covered with the coating layer 17, and that the hollow particles 13 and outer shells 14 were not exposed on the surface side. It was confirmed that the coating layer 17 had communicating holes 18 that communicated from the outside to the inside of the porous body 10.

[0187] Although the present embodiment has been described above using examples and comparative examples, the present embodiment is not limited to these examples and comparative examples, and various modifications are possible within the scope of the gist of the present embodiment. [Explanation of symbols]

[0188] 1. Analytical carrier 10 Porous materials 11 Skeleton 12 void 13 Hollow particles 14 Outer Shell 15 Cavity 17 Covering layer 18 Communication hole 20 Support 21 Support layer 22 Adhesive layer 23 Adhesive part 24 Auxiliary part 100 Sintered material 105 Aluminum substrate 110 Sintered body 113 Aluminum metal particles 117 Covering layer 120 Metallic parts 130 laminate

Claims

1. An analytical carrier comprising a porous body and a support that supports the porous body on one surface, the porous body includes a skeleton formed by an aggregation of a plurality of hollow particles and a plurality of voids surrounded by the skeleton, the hollow particles have an outer shell including an anodized coating containing aluminum oxide, and a cavity surrounded by the outer shell; the skeleton is formed by the outer shells of the plurality of hollow particles being continuous, the porosity of the porous body is 50% by volume or more and less than 100% by volume; The average pore diameter of the porous body is 0.1 μm or more and 20 μm or less, the support includes an adhesive layer that adheres to the porous body, The adhesive layer is adhered to the outer shell.

2. 2. The analytical carrier according to claim 1, wherein the adhesive layer comprises at least one material selected from the group consisting of a resin, an elastomer, a starch, and a protein.

3. 2. The analytical carrier according to claim 1, wherein the adhesive layer has a thickness of 1 μm or more and 1000 μm or less.

4. the adhesive layer has an adhesive portion that penetrates into the gap and the cavity and is in contact with the outer shell, and an auxiliary portion that is provided on the adhesive layer on the opposite side to the adhesive portion and is continuous with the adhesive portion, 2. The analytical carrier according to claim 1, wherein the thickness of the adhesive portion is 1 μm or more and 100 μm or less.

5. the support includes the adhesive layer and a support layer that supports the adhesive layer, 2. The analytical carrier according to claim 1, wherein the adhesive layer is interposed between the porous body and the support layer.

6. The analytical carrier according to claim 5 , wherein the support layer comprises at least one material selected from the group consisting of a resin, an elastomer, a metal, and paper.

7. The analytical carrier according to claim 1 , wherein the support has the porous body on one side, a first side, and a second side, a side opposite to the first side, that is exposed.

8. 2. The analytical carrier according to claim 1, wherein the mass ratio of metallic aluminum to the component constituting the porous body containing aluminum element is 0 or more and 0.1 or less.

9. the porous body includes a coating layer provided on a surface opposite to the support, separating the inside of the porous body where the skeleton and the voids exist from the outside of the porous body, the coating layer including an anodized film containing aluminum oxide; the coating layer has communicating holes that communicate the inside and outside of the porous body, the coating layer is formed continuously with the outer shell of the hollow particle, the cavities of the hollow particles communicate with the outside of the porous body through the communicating holes; The analytical carrier according to claim 1 .

10. 10. The analytical carrier according to claim 9, wherein the surface roughness Sa of the porous body is 0.01 μm or more and less than 1.8 μm.

11. 2. The analytical carrier according to claim 1, wherein the thickness of the outer shell is 40 nm or more and 1000 nm or less.

12. 2. The analytical carrier according to claim 1, wherein the thickness of the porous body is 20 μm or more and 190 μm or less.

13. The cumulative pore surface area of ​​the porous body having a pore diameter of 0.1 μm or more and 100 μm or less is 0.1 m 2 / cm 3 More than 20m 2 / cm 3 The analytical carrier according to claim 1, wherein:

14. 2. The analytical carrier according to claim 1, wherein the ratio of the cumulative pore surface area of ​​pores having a pore diameter of 0.1 μm or more and less than 1 μm to the cumulative pore surface area of ​​pores having a pore diameter of 1 μm or more and 10 μm or less is 0.1 or more and 10 or less.

15. 2. The analytical carrier according to claim 1, wherein the time required for water to be drawn up to a height of 4 cm by capillary action is 400 seconds or less.

16. The analytical carrier in which the porous body has dried is placed on a white reflection standard with which a measuring instrument is calibrated, and the surface of the porous body in the analytical carrier is measured with the measuring instrument. ＊ a ＊ b ＊ L in color system ＊ The analytical carrier according to claim 1, wherein the value is 75 or more.

17. The analytical carrier with the porous body absorbing water is placed on a white reflection standard with which a measuring instrument is calibrated, and the L ＊ a ＊ b ＊ L in color system ＊ The analytical carrier according to claim 1, wherein the value is 75 or more.

18. The analytical carrier with the porous body absorbing water is placed on a white reflection standard with which a measuring instrument is calibrated, and the L ＊ a ＊ b ＊ L in color system ＊ The analytical support according to claim 1, wherein the value is less than 75.

19. The analytical carrier described in claim 1, wherein 10 g weights are attached to both ends of the analytical carrier cut to 10 mm x 100 mm, the analytical carrier is placed on a stainless steel rod at the longitudinal center of the analytical carrier so that the porous body is in contact with the stainless steel rod, the analytical carrier is lifted until the weights at both ends of the analytical carrier float and held there for 10 seconds, and then the stainless steel rod is returned to its original position, and the porous body does not peel off.

20. An immunochromatographic test strip comprising the analytical carrier according to any one of claims 1 to 19.

21. A method for producing an analytical carrier comprising a porous body and a support that supports the porous body on one surface, the porous body includes a skeleton formed by an aggregation of a plurality of hollow particles and a plurality of voids surrounded by the skeleton, the hollow particles have an outer shell including an anodized coating containing aluminum oxide, and a cavity surrounded by the outer shell; the skeleton is formed by the outer shells of the plurality of hollow particles being continuous, The manufacturing method includes: a sintering step of sintering a plurality of aluminum metal particles on an aluminum base material to obtain a sintered material including the aluminum base material and a sintered body in which the aluminum metal particles are sintered and laminated on the aluminum base material; an anodizing step of anodizing the sintered material to form the outer shell including the anodized coating on the surface of the aluminum metal particle; a dissolving step of dissolving the aluminum metal particles surrounded by the outer shell; Including, In the manufacturing method, the anodizing step and the dissolving step are repeated to form a metal member in which the porous body is laminated on the aluminum base material, The manufacturing method includes: a lamination step of adhering the porous body of the metal member to the support to form a laminate in which the aluminum base, the porous body, and the support are laminated in this order; a peeling step of dissolving the aluminum base and peeling the aluminum base from the laminate; Including, The aluminum metal particles contain at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys, the aluminum base contains at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and an aluminum alloy; The average particle size of the plurality of aluminum metal particles is 0.1 μm or more and 20 μm or less, The packing ratio of the sintered body is 10% by volume or more and 60% by volume or less. the support includes an adhesive layer that adheres to the porous body, In the laminating step, the adhesive layer is adhered to the outer shell.

22. In the anodizing step, a coating layer including an anodized film containing aluminum oxide is formed on the surface of the aluminum base material on which the aluminum metal particles are laminated, 22. The method for producing an analytical carrier according to claim 21, wherein in the peeling step, the aluminum base is dissolved with an acid solution or an alkaline solution to separate the coating layer formed on the surface of the aluminum base opposite to the porous body from the porous body.

23. 23. The method for producing an analytical support according to claim 21 or 22, wherein the electrolytic solution used in the anodizing step contains at least one selected from the group consisting of citric acid, boric acid, phosphoric acid, sulfuric acid, oxalic acid, and salts thereof.

24. The method for producing an analytical support according to claim 21 or 22, wherein the anodizing step and the dissolving step are alternately repeated, and the number of repetitions of the anodizing step and the dissolving step is 2 or more and 20 or less.

25. The dissolving solution for dissolving the aluminum metal particles in the dissolving step is At least one acid selected from the group consisting of phosphoric acid, sulfuric acid, nitric acid, and salts thereof, or The method for producing an analytical support according to claim 21 or 22, which contains at least one alkali selected from the group consisting of sodium hydroxide and potassium hydroxide.

26. 23. The method for producing an analytical support according to claim 21, wherein the stripping solution used in the stripping step contains at least one alkali selected from the group consisting of sodium hydroxide and potassium hydroxide.

27. 27. The method for producing an analytical carrier according to claim 26, wherein the stripping solution used in the stripping step contains at least one organic acid selected from the group consisting of citric acid and gluconic acid.

28. 23. The method for producing an analytical carrier according to claim 21, wherein the stripping solution used in the stripping step contains at least one acid selected from the group consisting of phosphoric acid, sulfuric acid, and nitric acid.

29. The method for producing an analytical carrier according to claim 21 or 22, wherein the temperature of the stripping solution in the stripping step is 30°C or higher and 70°C or lower.

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