Manufacturing method and use of honeybee repellent

Abstract

To provide a honeybee repellent, and manufacturing method and use of the honeybee repellent.SOLUTION: There are provided a honeybee repellant, and manufacturing method and use thereof. The manufacturing method of the honeybee repellant includes the following steps of: preparing a mixed solution with a weight ratio of sugar to water of 1.5:1; placing an effective amount of ants into the mixed solution and maintaining it for a specific soaking time; after removing the ants, obtaining the honeybee repellant. Since the honeybee repellant is obtained by soaking the ants, the smell emitted by the honeybee repellant causes the honeybees to exhibit aversion or avoidance behavior, thereby alleviating the problem of the honeybees accidentally ingesting a harmful insect repellent bait.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a honeybee repellent, and more particularly to a honeybee repellent that reduces the possibility of ants being accidentally eaten by honeybees by immersing them in the repellent, and a method for producing the same and its use. [Background technology]

[0002] Honeybees are pollinators with extremely high economic value, are crucial for ecological balance and crop growth, and are one of the keys to sustainable agricultural production. Beekeepers must be wary of pests and diseases such as parasites and viruses, but another problem that has been plaguing beekeepers in recent years is the problem of ants. Ants actively attack worker bees that are alone on the ground and may even enter beehives to steal honey. In mild cases, this only disrupts the colony, but in severe cases, it can wipe out the entire colony.

[0003] Currently, the main insecticides used to control mosquitoes and ants are sugar baits based on sugar water. Therefore, if pesticide bait is placed around apiaries, there is a risk that the bees may accidentally ingest it, causing harm to the bees or leaving chemical residues that could contaminate the honey. Therefore, there is room for improvement in conventional sugar water baits. Summary of the Invention [Problem to be solved by the invention]

[0004] The above-mentioned prior art has the drawback that honeybees may accidentally ingest the sugar water-based pesticide, causing harm to the bees or contaminating the honey, resulting in drug residue. [Means for solving the problem]

[0005] In order to solve the above-mentioned problems, the present invention provides a bee repellent that reduces the possibility of bees accidentally ingesting it, as well as a method for producing the same and its use.

[0006] In the bee repellent and its manufacturing method and use according to the present invention, the manufacturing method of the bee repellent includes the following steps: Prepare a mixed solution with sugar and water in a weight ratio of 1.5:1. Put an effective number of ants into the mixed solution and maintain a certain immersion time; After taking out the ants, you will get some bee repellent.

[0007] In one embodiment, the present invention provides a honeybee repellent prepared by the above-described method.

[0008] In one embodiment, the present invention uses the above-described honeybee repellent in the manufacture of pest insect control baits.

[0009] As described above, the bee repellent of the present invention is prepared by immersing ants in the repellent, and therefore the odor emitted by the bee repellent causes disgust or avoidance in the bees, significantly reducing their willingness to eat the sugar water or preventing them from eating it. Therefore, the repellent can be used as a bait for exterminating harmful insects. For example, it can be applied to the extermination of harmful insects that feed on sugar water, such as ants, mosquitoes, and midges, and can alleviate the problem of honeybees accidentally ingesting harmful insect bait. [Brief explanation of the drawings]

[0010] [Figure 1] 1 is a block flow chart of a method for producing a honeybee repellent according to the present invention. [Figure 2A] This is an immersion diagram of the control group, Pterygota ants and Pterygota ants of the genus Hardy ant, in Test 1. [Figure 2B] This is an immersion diagram of the control group, Pterygota ants and Pterygota ants of the genus Hardy ant, in Test 2. [Figure 2C] This is an immersion diagram of the control group, Pterygota ants and Pterygota ants of the genus Hardy ant, in Test 3. [Figure 2D]This is an immersion diagram of the control group, Pterygota ants and Pterygota ants of the genus Hardy ant, in Test 4. [Figure 3] This is a graph showing the change in honeybee predation rate from test 1 to test 4. DETAILED DESCRIPTION OF THE INVENTION

[0011] (One embodiment) To make the above and other objects, features, and advantages of the present invention clearer and more easily understandable, the following detailed description will be given of embodiments of the present invention in conjunction with the accompanying drawings.

[0012] Referring to FIGS. 1 to 3, the method for producing a honeybee repellent according to the present invention includes the following steps.

[0013] Step S1: Prepare a mixed solution of sugar and water in a weight ratio of 1.5:1, where the sugar is fructose, xylitol, sucrose, maltitol or glucose.

[0014] Step S2: Add an effective number of ants to the mixed solution and maintain a certain immersion time, the population volume ratio of the effective number of ants to the mixed solution (ml) is 2.5-6.3, and the immersion time is at least 72 hours (i.e., 3 days).

[0015] Step S3: After removing the ants, obtain the bee repellent. Honeybee repellents are used in the manufacture of pest insect baits.

[0016] The population-to-capacity ratio is 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.8, 6.0, 6.1, 6.2, or 6.3.

[0017] The soaking time is 3 days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days, 15 days, 16 days, 17 days, 18 days, 19 days, 20 days, 21 days, 22 days, 23 days, 24 days, 25 days, 26 days, 27 days, 28 days, 29 days, or 30 days.

[0018] The ant is a species of the fire ant (Anoplolepis gracilipes) or the hard ant (Dolichoderus thoracicus), or a combination thereof.

[0019] The test steps of the embodiment are as follows: Sucrose solutions with a weight ratio of sucrose to water of 1.5:1 and a concentration of 150% wt / wt were prepared, and then different numbers or species of ants were added. The ratio of ant population to solution volume for each test is shown in Table 1 below. After at least three days of soaking in the solution, the sucrose solution in which the ants were soaked will be tested to determine whether it is repellent to honeybees, and at the same time, its effective concentration range will be determined.

[0020] In each test, three different types of sucrose solution are placed in each of the linked pillboxes (approximately 10 ml). The different types of solutions are placed one pillbox apart and the pillboxes are placed in front of the bee entrance location of the hive. The honeybees used in the repellent behavior test are European honeybees (Apis mellifera).

[0021] After the solutions are placed, the number of bees remaining and feeding on each sucrose solution is observed and recorded every two minutes. Total observation time is 10 minutes. All tests are repeated at least four times. When repeated, the relative positions of each sucrose solution are swapped.

[0022] After the test is completed, the average number of bees in each type of sucrose solution in each test is calculated. A one-way ANOVA, followed by a post-hoc Tukey's HSD test, was used to examine whether there were differences in the honeybees' feeding preferences for each type of sucrose solution.

[0023] Test results: Figure 2A shows the results of Test 1, Figure 2B shows the results of Test 2, Figure 2C shows the results of Test 3, and Figure 2D shows the results of Test 4. Each experimental group included a control group of 10, a group of fire ants called 11, and a group of solacic ants called 12. The groups were not fixed in their order of location in each experiment.

[0024] (Table 1) The ratio of the number of ants to the volume of solution and the number of days of immersion in each test TIFF2025163885000002.tif66145*The weight ratio of sucrose to water is 1.5:1

[0025] (Table 2) Number of honeybees feeding on different types of sucrose solutions TIFF2025163885000003.tif47145

[0026] Values ​​are means ± standard error. Different letters in the same column indicate significant differences between the means (Tukey's HSD test, p < 0.05).

[0027] The ratio of ant population to solution volume in each test is shown in Table 1. As can be seen in Table 1, the test results show the sucrose solutions and their ratios (population-volume ratios) for various test ant species (Coleoptera and C. solacicidae).

[0028] As can be seen from Table 2, there was a clear repellent effect on the honeybees (statistically significant difference compared to the pure sucrose control group 10).

[0029] As shown in FIG. 3 and Table 2, the results of Tests 1 to 4 reveal the following. When the population-capacity ratio is less than 30, the repellent effect of the genus Sclerotinia ant is slightly better than that of the red ant. As can be seen from Test 4, when the population-capacity ratio is greater than 30, the repellent effect of the red fire ant is slightly better than that of the sclerotinic ant. Since honeybees are repellent to both sucrose solutions of the two ant species, increasing the population-volume ratio of the ants of the genus Formicaria or the ant of the genus Cephalosporin and the duration of immersion increases the repellency to honeybees.

[0030] In test 4, where the volume ratio was 33.3, after 10 days of immersion, honeybee predation was reduced by 78% (fire ants) and 70% (common ants). Therefore, test 4 was found to be the best formulation, reducing honeybee predation by more than 70%.

[0031] As mentioned above, the advantages of the present invention are as follows: 1. Highly effective in repelling bees: After dipping the ants in sugar water, the bee repellent takes into consideration the ecology and protection of bees. It can effectively eliminate ants while significantly reducing the bees' desire to prey on the sugar water, preventing them from accidentally eating it. This not only protects crops but also ensures the safety of bees, reduces interference with bees and negative impacts on agriculture, and protects the bees' living environment. 2. Eco-friendly: The bee repellent of the present invention is prepared from ants and sugar water and does not contain any harmful chemicals, making it environmentally friendly and avoiding any adverse effects on the ecosystem. 3. Low material costs: Whether it's ants or sugar water, the ingredients are common and inexpensive, and can be easily obtained and prepared.

[0032] The above embodiments are merely specific and detailed descriptions of several ways of implementing the present invention, and are not intended to limit the scope of the claims. Any slight modifications and improvements made by persons skilled in the art without departing from the concept of the present invention will also fall within the scope of protection of the present invention.

[0033] The above-described embodiments of the present invention are not intended to limit the scope of the present invention, and the scope of protection provided by the present invention is determined by the following claims. [Explanation of symbols]

[0034] Steps S1~S3. 10 control group 11 Group of Long-legged Fire Ants 12. The group of ants in the genus Pyrophorus

Claims

1. 1. A method for producing a honeybee repellent, comprising the steps of: Prepare a mixed solution of sugar and water in a weight ratio of 1.5:

1. Put an effective amount of ants into the mixed solution and maintain a certain immersion time; After taking out the ants, obtain the bee repellent. characterized in that How to make honeybee repellent.

2. The population volume ratio of the effective number of ants to the mixed solution (ml) is 2.5-6.

3. characterized in that A method for producing the honeybee repellent according to claim 1.

3. The soaking time is at least 72 hours. characterized in that A method for producing the honeybee repellent according to claim 1.

4. The sugar is fructose, xylitol, sucrose, maltitol or glucose. characterized in that A method for producing the honeybee repellent according to claim 1.

5. The ant is a species of the fire ant (Anoplolepis gracilipes) or the ant (Dolichoderus thoracicus), or a combination thereof. characterized in that A method for producing the honeybee repellent according to claim 1.

6. Produced by the method of any one of claims 1 to 5. characterized in that Bee repellent.

7. The honeybee repellent according to claim 6 is used in the production of a bait for controlling harmful insects. characterized in that Uses for bee repellents.

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