Bridged ring substituted heteroaryl-pyran derivatives and uses thereof

Abstract

The present invention discloses bridged ring substituted heteroaryl-pyran derivatives and uses thereof, and in particular, discloses a compound represented by formula (VI) or a pharma- ceutically acceptable salt thereof. [C1] TIFF2025504491000283.tif41156

Description

[Technical field]

[0001] The present application, China Patent Application Publication No. 202210074707.1, filed on January 21, 2022; China Patent Application Publication No. 202210082274.4, filed on January 24, 2022; China Patent Application Publication No. 202210254285.6, filed on March 15, 2022; China Patent Application Publication No. 202210346571.5, filed on March 31, 2022; China Patent Application Publication No. 202210642158.3, filed on June 7, 2022; China Patent Application Publication No. 202210813772.1, filed on July 11, 2022; China Patent Application Publication No. 202210964127.X, filed on August 11, 2022; This application claims priority from Chinese Patent Application Publication No. 202310029317.7, filed on January 9, 2023.

[0002] <Technical field> The present disclosure relates to bridged ring-substituted heteroaryl fused pyran derivatives and uses thereof, specifically, to a compound represented by formula (VI) or a pharma- ceutically acceptable salt thereof. [Background technology]

[0003] Mutations in the RAS oncogene are the most common activating mutations in human cancers, occurring in 30% of human tumors. The RAS gene family includes three subtypes (KRAS, HRAS, and NRAS), and 85% of RAS-driven cancers are caused by mutations in the KRAS subtype. KRAS mutations are commonly found in solid tumors such as lung adenocarcinoma, pancreatic ductal carcinoma, and colorectal cancer. In KRAS-mutated tumors, 80% of oncogenic mutations occur at codon 12, with the most common mutations including p.G12D (41%), p.G12V (28%), and p.G12C (14%).

[0004] KRAS is a murine sarcoma virus oncogene and an important member of the RAS protein. KRAS is like a molecular switch that can control and regulate the pathway of cell proliferation under normal conditions. After mutation, the KRAS gene can independently transmit growth and proliferation signals to downstream pathways independent of upstream growth factor receptor signals, leading to uncontrolled cell proliferation and tumor progression. Meanwhile, the presence or absence of mutations in the KRAS gene is also an important indicator of tumor prognosis.

[0005] Currently, small molecules that directly target KRAS mutations are G12C The company is primarily focused on the area of ​​KRAS. Amgen's AMG510 and Mirati Therapeutic's MRTX849 have been shown to be candidates for KRAS inhibitors in clinical studies. G12C The treatment has shown favorable outcomes for patients with tumors that have KRAS mutations. G12D No small molecules targeting KRAS have entered clinical trials. G12D Patients with mutated tumors have yet to benefit from precision medicine.

[0006] The present application is directed to KRAS G12D A series of small molecule inhibitors targeting and methods for their preparation are disclosed. Summary of the Invention [Means for solving the problem]

[0007] The present disclosure relates to a compound of formula (VI) [ka] or a pharma- ceutically acceptable salt thereof,

[0008] During the ceremony,

[0009] [ka] is selected from a single bond and a double bond;

[0010] T1 is CR a and N,

[0011] T2 is selected from CH and N;

[0012] L1 is selected from O and S;

[0013] R1 is phenyl, naphthyl, [ka] The above phenyl, naphthyl, [ka] each independently represents 1, 2, 3, 4, or 5 R b Optionally replaced by

[0014] R2 is H;

[0015] R3 is selected from H, F, CN, CH3, and OCH3, CH3 and OCH3 being optionally substituted with 1, 2, or 3 halogens;

[0016] Alternatively, R2 and R3 together with the atom to which they are attached form a phenyl group or a 5-6 membered heteroaryl group, said phenyl group or 5-6 membered heteroaryl group being optionally substituted with one, two or three halogens;

[0017] R4 is absent or selected from H, CH3 and -CH=CH2, wherein CH3 and -CH=CH2 are optionally substituted with 1, 2 or 3 halogens;

[0018] Alternatively, R3 and R4, together with the atoms to which they are attached, can be [ka] Form the above [ka] is one or two R c Optionally replaced by

[0019] Alternatively, R3 and R4 together with the atom to which they are attached form a cyclopropyl group optionally substituted with 1, 2 or 3 halogens;

[0020] R a is selected from H and CN;

[0021] Each R b are H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl, 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogen;

[0022] Each R c is independently selected from F, Cl, Br, I, CN, CH3, and OCH3;

[0023] m is selected from 0 and 1; or a pharma- ceutically acceptable salt thereof.

[0024] The present disclosure relates to a compound represented by formula (P-1): [ka] or a pharma- ceutically acceptable salt thereof,

[0025] During the ceremony,

[0026] [ka] is selected from a single bond and a double bond;

[0027] L1 is selected from O and S;

[0028] R1 is phenyl, naphthyl, 5- to 10-membered heteroaryl; [ka] phenyl, naphthyl, 5- to 10-membered heteroaryl, [ka] each independently represents 1, 2, 3, 4, or 5 R b and R5 is optionally substituted with [ka] Selected from the above [ka] each independently represents 1, 2, 3, 4, or 5 R d Optionally replaced by

[0029] Alternatively, R1 is a 5-10 membered heteroaryl, which is selected from 1, 2, 3, 4, or 5 R b and R5 is optionally substituted with [ka] And the above [ka] 1, 2, 3, 4 or 5 R d Optionally replaced by

[0030] Each R b are H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl, 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 R;

[0031] Each R d is independently selected from F, Cl, Br, I, CN, CH3, and OCH3;

[0032] Each R is independently selected from halogen and D, or a pharma- ceutically acceptable salt thereof.

[0033] The present disclosure relates to a compound represented by formula (P-2): [ka] or a pharma- ceutically acceptable salt thereof,

[0034] During the ceremony,

[0035] [ka] is selected from a single bond and a double bond;

[0036] L1 is selected from O and S;

[0037] Each Rb are F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl, 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 R;

[0038] each R is independently selected from halogen and D;

[0039] v is selected from 1, 2, 3, 4, and 5;

[0040] However, there must be at least one R b is substituted with 1, 2, 3, 4 or 5 D, or a pharma- ceutically acceptable salt thereof.

[0041] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, independently selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4 or 5 halogens, and the other variables are as defined herein.

[0042] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2F, CF2H, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and the other variables are as defined herein.

[0043] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, independently selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4, or 5 R, and other variables are as defined herein.

[0044] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CD3, CH2F, CF2H, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and the other variables are as defined herein.

[0045] In some embodiments of the present disclosure, R1 is [ka] and other variables are as defined herein.

[0046] In some embodiments of the present disclosure, R1 is [ka] and other variables are as defined herein.

[0047] In some embodiments of the present disclosure, R1 is [ka] and other variables are as defined herein.

[0048] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0049] In some embodiments of the present disclosure, R3 is selected from H, F, CN, CH3, and OCH3, and other variables are as defined herein.

[0050] In some embodiments of the present disclosure, R2 and R3 together with the atom to which they are attached form phenyl, furanyl, and pyridinyl, which are optionally substituted with one F, and other variables are as defined herein.

[0051] In some embodiments of the present disclosure, R4 is absent or selected from H, CH2F, and -CH=CH2, and other variables are as defined herein.

[0052] In some embodiments of the present disclosure, R3 and R4, together with the atom to which they are attached, are [ka] where the other variables are as defined herein.

[0053] In some embodiments of the present disclosure, R3 and R4, together with the atom to which they are attached, are [ka] where the other variables are as defined herein.

[0054] In some embodiments of the present disclosure, R5 is [ka] and other variables are as defined herein.

[0055] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0056] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0057] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0058] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0059] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0060] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0061] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0062] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] and

[0063] During the ceremony,

[0064] [ka] is selected from a single bond and a double bond;

[0065] T1, T2, R1, R2, R3, R4, L1 and m are as defined herein.

[0066] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] and

[0067] During the ceremony,

[0068] [ka] is selected from a single bond and a double bond;

[0069] T1, T2, R1, R2, R3, R4, L1 and m are as defined herein;

[0070] However, the structural part [ka] but [ka] If [ka] teeth [ka] instead, R2 is H, R3 is selected from H, F, CN, CH3, and OCH3, wherein CH3 and OCH3 are optionally substituted with 1, 2 or 3 halogens, and R4 is selected from H and CH3.

[0071] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] and

[0072] During the ceremony,

[0073] [ka] is selected from a single bond and a double bond;

[0074] structural part [ka] teeth, [ka] is selected from

[0075] R2 is H;

[0076] R3 is selected from H, F, CN, CH3, and OCH3, wherein CH3 and OCH3 are optionally substituted with 1, 2, or 3 halogens;

[0077] Alternatively, R2 and R3 together with the atom to which they are attached form a phenyl group or a 5-6 membered heteroaryl group, said phenyl group or 5-6 membered heteroaryl group being optionally substituted with one, two or three halogens;

[0078] R4 is -CH=CH2, wherein the -CH=CH2 is optionally substituted with 1, 2 or 3 halogens;

[0079] Alternatively, R3 and R4, together with the atoms to which they are attached, can be [ka] Form the above [ka] is one or two R c Optionally replaced by

[0080] Alternatively, R3 and R4 together with the atom to which they are attached form a cyclopropyl group optionally substituted with 1, 2 or 3 halogens;

[0081] T1, T2, R1, each R c , L1 and m are as defined herein.

[0082] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0083] In some embodiments of the present disclosure, T1 is N, and other variables are as defined herein.

[0084] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0085] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0086] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] is selected from

[0087] During the ceremony,

[0088] [ka] is selected from a single bond and a double bond;

[0089] Each R b and L1 is as defined herein;

[0090] n is selected from 0, 1, 2, 3, 4 and 5.

[0091] In some embodiments of the present disclosure, the structural moiety [ka] [ka] and other variables are as defined herein.

[0092] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0093] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] is selected from

[0094] During the ceremony,

[0095] Each R b and L1 is as defined herein;

[0096] n is selected from 0, 1, 2, 3, 4 and 5.

[0097] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] is selected from

[0098] During the ceremony,

[0099] Each R b and L1 is as defined herein;

[0100] n is selected from 0, 1, 2, 3, 4 and 5.

[0101] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] is selected from

[0102] During the ceremony,

[0103] [ka] is selected from a single bond and a double bond;

[0104] T1, T2, R2, R3, R4 and L1 are as defined herein;

[0105] R b1 , R b2 , R b3 , R b4 , R b5 , R b6 and R b7 are H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl, 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 The cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogens.

[0106] In some embodiments of the present disclosure, R b1 , R b2 ,Rb3 , R b4 , R b5 , R b6 , R b7 and R b8 are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, independently selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4, or 5 R, and other variables are as defined herein.

[0107] In some embodiments of the present disclosure, R b1 , R b2 ,R b3 , R b4 , R b5 , R b6 , R b7 and R b8 are H, F, Cl, Br, I, OH, NH2, CN, CH3, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and the other variables are as defined herein.

[0108] The present disclosure also provides a compound represented by formula (IV) or a pharma- ceutically acceptable salt thereof: [ka]

[0109] During the ceremony,

[0110] [ka] is selected from a single bond and a double bond;

[0111] T1 is CR a and N,

[0112] T2 is selected from CH and N;

[0113] L1 is selected from O and S;

[0114] R1 is phenyl, naphthyl, [ka] The above phenyl, naphthyl, [ka] each independently represents 1, 2, 3, 4, or 5 R b Optionally replaced by

[0115] R2 is H;

[0116] R3 is selected from H, F, CN, and OCH3, wherein OCH3 is optionally substituted with 1, 2, or 3 halogens;

[0117] Alternatively, R2 and R3 together with the atom to which they are attached form a phenyl group or a 5-6 membered heteroaryl group, the phenyl group and the 5-6 membered heteroaryl group being optionally substituted with one, two or three halogens;

[0118] R4 is absent or selected from H, CH3 and -CH=CH2, wherein CH3 and -CH=CH2 are optionally substituted with 1, 2 or 3 halogens;

[0119] Alternatively, R3 and R4, together with the atoms to which they are attached, can be [ka] Form the above [ka] is optionally substituted with 1 or 2 halogens;

[0120] Alternatively, R3 and R4 together with the atom to which they are attached form a cyclopropyl group optionally substituted with 1, 2 or 3 halogens;

[0121] R a is selected from H and CN;

[0122] Each R b are H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl, 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 The cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogens.

[0123] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, independently selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4 or 5 halogens, and the other variables are as defined herein.

[0124] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2F, CF2H, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and the other variables are as defined herein.

[0125] In some embodiments of the present disclosure, R1 is [ka] and other variables are as defined herein.

[0126] In some embodiments of the present disclosure, R3 is selected from H, F, CN, and OCH3, and other variables are as defined herein.

[0127] In some embodiments of the present disclosure, R2 and R3 together with the atom to which they are attached form a phenyl, furanyl, or pyridinyl, wherein the phenyl, furanyl, and pyridinyl are optionally substituted with one F, and the other variables are as defined herein.

[0128] In some embodiments of the present disclosure, R4 is absent or selected from H, CH2F, and -CH=CH2, and other variables are as defined herein.

[0129] In some embodiments of the present disclosure, R3 and R4, together with the atom to which they are attached, are [ka] where the other variables are as defined herein.

[0130] In some embodiments of the present disclosure, R3 and R4, together with the atom to which they are attached, are [ka] where the other variables are as defined herein.

[0131] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0132] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] is selected from

[0133] During the ceremony,

[0134] [ka] is selected from a single bond and a double bond;

[0135] T1, T2, R2, R3, R4 and L1 are as defined herein;

[0136] R b1 , R b2 , R b3 , R b4 , R b5 , R b6 and R b7are each independently H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl; 1-3 Alkyl, C 1-3 Alkoxy, C 2-4 Alkynyl, C 2-4 Alkynyl-cyclopropyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 The cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogens.

[0137] In some embodiments of the present disclosure, R b1 , R b2 ,R b3 , R b4 , R b5 , R b6 , R b7 and R b8 are each independently H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4, or 5 R, and other variables are as defined herein.

[0138] In some embodiments of the present disclosure, R b1 , R b2 , R b3 , R b4 , R b5, R b6 , R b7 and R b8 are each independently H, F, Cl, Br, I, OH, NH2, CN, CH3, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and other variables are as defined herein.

[0139] The present disclosure also provides a compound represented by formula (IV) or a pharma- ceutically acceptable salt thereof: [ka]

[0140] During the ceremony,

[0141] [ka] is selected from a single bond and a double bond;

[0142] T1 is CR a and N,

[0143] T2 is selected from CH and N;

[0144] L1 is selected from O and S;

[0145] R1 is selected from phenyl and naphthyl, each of which independently has 1, 2, 3, 4, or 5 R b Optionally replaced by

[0146] R2 is H;

[0147] R3 is selected from H, F and CN;

[0148] Alternatively, R2 and R3 together with the atoms to which they are attached form a phenyl group;

[0149] R4 is absent or selected from H and -CH=CH2;

[0150] R a is selected from H and CN;

[0151] Each R b are H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl, 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 The cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogens.

[0152] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, independently selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4 or 5 halogens, and the other variables are as defined herein.

[0153] In some embodiments of the present disclosure, each R bare H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2F, CF2H, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and the other variables are as defined herein.

[0154] In some embodiments of the present disclosure, R1 is [ka] and other variables are as defined herein.

[0155] In some embodiments of the present disclosure, the structural moiety [ka] teeth [ka] and other variables are as defined herein.

[0156] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] is selected from

[0157] During the ceremony,

[0158] [ka] is selected from a single bond and a double bond;

[0159] T1, T2, R2, R3, R4 and L1 are as defined herein;

[0160] R b1, R b2 ,R b3 , R b4 , R b5 , R b6 and R b7 are each independently H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl; 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 The cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogens.

[0161] In some embodiments of the present disclosure, R b1 , R b2 ,R b3 , R b4 , R b5 , R b6 , R b7 and R b8 are each independently H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4, or 5 R, and other variables are as defined herein.

[0162] In some embodiments of the present disclosure, R b1 , R b2 , Rb3 , R b4 , R b5 , R b6 , R b7 and R b8 are each independently H, F, Cl, Br, I, OH, NH2, CN, CH3, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and other variables are as defined herein.

[0163] The present disclosure also provides a compound represented by formula (III) or a pharma- ceutically acceptable salt thereof: [ka]

[0164] During the ceremony,

[0165] [ka] is selected from a single bond and a double bond;

[0166] T1 is CR a and N,

[0167] T2 is selected from CH and N;

[0168] L1 is selected from O and S;

[0169] R1 is selected from phenyl and naphthyl, each of which independently has 1, 2, 3, 4, or 5 R b Optionally replaced by

[0170] R2 is H;

[0171] R3 is selected from H and F;

[0172] Alternatively, R2 and R3 together with the atoms to which they are attached form a phenyl group;

[0173] R a is selected from H and CN;

[0174] Each R b are H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl, 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 The cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogens.

[0175] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, independently selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4 or 5 halogens, and the other variables are as defined herein.

[0176] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2F, CF2H, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and the other variables are as defined herein.

[0177] In some embodiments of the present disclosure, R1 is [ka] and other variables are as defined herein.

[0178] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] is selected from

[0179] During the ceremony,

[0180] [ka] is selected from a single bond and a double bond;

[0181] T1, T2, R2, R3 and L1 are as defined herein;

[0182] R b1 , R b2 ,R b3 , R b4 , R b5 , R b6 and R b7 are each independently H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl; 1-3 Alkyl, C 1-3Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 The cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogens.

[0183] In some embodiments of the present disclosure, R b1 , R b2 ,R b3 , R b4 , R b5 , R b6 , R b7 and R b8 are each independently H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4, or 5 R, and other variables are as defined herein.

[0184] In some embodiments of the present disclosure, R b1 , R b2 , R b3 , R b4 , R b5 , R b6 , R b7 and R b8 are each independently H, F, Cl, Br, I, OH, NH2, CN, CH3, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and other variables are as defined herein.

[0185] The present disclosure also provides a compound represented by formula (I) or a pharma- ceutically acceptable salt thereof: [ka]

[0186] During the ceremony,

[0187] [ka] is selected from a single bond and a double bond;

[0188] T1 is CR a and N,

[0189] L1 is selected from O and S;

[0190] R1 is selected from phenyl and naphthyl, each of which independently has 1, 2, 3, 4, or 5 R b Optionally replaced by

[0191] R a is selected from H and CN;

[0192] Each R b are H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl, 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 The cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogens.

[0193] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, independently selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4 or 5 halogens, and the other variables are as defined herein.

[0194] In some embodiments of the present disclosure, each R b are H, F, Cl, Br, I, OH, NH2, CN, CH3, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and the other variables are as defined herein.

[0195] In some embodiments of the disclosure, a compound or a pharma- ceutically acceptable salt thereof is provided, the compound comprising: [ka] is selected from

[0196] During the ceremony,

[0197] [ka] is selected from a single bond and a double bond;

[0198] T1 and L1 are as defined herein;

[0199] Rb1 , R b2 ,R b3 , R b4 , R b5 , R b6 and R b7 are each independently H, F, Cl, Br, I, OH, NH2, CN, C 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 cycloalkyl; 1-3 Alkyl, C 1-3 Alkoxy, C 2-3 Alkynyl, C 2-3 Alkenyl, -C(=O)C 1-3 Alkyl and C 3-5 The cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogens.

[0200] In some embodiments of the present disclosure, R b1 , R b2 ,R b3 , R b4 , R b5 , R b6 , R b7 and R b8 are each independently H, F, Cl, Br, I, OH, NH2, CN, CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl, selected from CH3, CH2CH3, OCH3, OCH2CH3, -CH=CH2, [ka] and cyclopropyl is optionally substituted with 1, 2, 3, 4, or 5 R, and other variables are as defined herein.

[0201] In some embodiments of the present disclosure, R b1 , R b2, R b3 , R b4 , R b5 , R b6 , R b7 and R b8 are each independently H, F, Cl, Br, I, OH, NH2, CN, CH3, CF3, CH2CH3, CF2CF3, -CH=CH2, [ka] and cyclopropyl, and other variables are as defined herein.

[0202] In Example 8 of the present disclosure, the hydrochloride salt of compound 8 was prepared from compound 8-3 by steps 6 and 7, compound 8-3 was prepared from compound 8-2 and compound 1-12B by step 5, and compound 8-2 and compound 1-12B were prepared from compound 8-1A and compound 1-11B, respectively.

[0203] In Example 8 of the present disclosure, the hydrochloride salt of compound 8 was prepared from compound 8-1A, which was analyzed by SFC (chromatographic column: Chiralpak IH-3, 100 x 4.6 mm ID, 3 μm; mobile phase: A (supercritical CO2) and B (ethanol, containing 0.1% isopropylamine); gradient: B% = 10-50%, 4 min; flow rate: 3.4 mL / min; wavelength: 220 nm; pressure: 2000 psi) and showed a retention time of 1.489 min.

[0204] In Example 8 of the present disclosure, the hydrochloride salt of compound 8 was prepared from compound 1-11B, which was analyzed by SFC (chromatographic column: (S,S) Whelk-O1 100×4.6 mm ID, 5.0 μm; mobile phase: A: supercritical CO2, B: [75% isopropanol / 25% acetonitrile / 0.05% diethylamine]; B%: 50%-50%, flow rate: 2.5 mL / min) and showed a retention time of 3.317 min, while its enantiomer had a retention time of 2.536 min.

[0205] In Example 8 of the present disclosure, compound 8 was prepared from compound 1-11B, which was analyzed by SFC (chromatographic column: (S,S) Whelk-O1 100×4.6 mm ID, 5.0 μm; mobile phase: A: supercritical CO2, B: [75% isopropanol / 25% acetonitrile / 0.05% diethylamine]; B%: 50%-50%, flow rate: 2.5 mL / min) and showed a retention time of 3.317 min, while its enantiomer had a retention time of 2.536 min.

[0206] In Example 8 of the present disclosure, compound 1-11B was analyzed by SFC (chromatographic column: (S,S) Whelk-O1 100×4.6 mm ID, 5.0 μm; mobile phase: A: supercritical CO2, B: [75% isopropanol / 25% acetonitrile / 0.05% diethylamine]; B%: 50%-50%, flow rate: 2.5 mL / min) and showed a retention time of 3.317 min, and its enantiomer had a retention time of 2.536 min. The hydrochloride salt of compound 8 was synthesized from 1-11B by the process described in Example 8.

[0207] Some other solutions of the present disclosure are obtained by combining any of the above variables.

[0208] The present disclosure provides a compound of the formula: [ka] [ka] [ka] [ka] [ka] [ka] or a pharma- ceutically acceptable salt thereof.

[0209] The present disclosure provides a compound of the formula: [ka] [ka] [ka] [ka] [ka] [ka] or a pharma- ceutically acceptable salt thereof.

[0210] The present disclosure provides a compound of the formula: [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] or a pharma- ceutically acceptable salt thereof.

[0211] The present disclosure also provides G12D There is also provided the use of the compound, or a pharma- ceutically acceptable salt thereof, in the manufacture of a medicament for treating a disease associated with a mutation.

[0212] The present disclosure also provides the use of the above compound or a pharma- ceutically acceptable salt thereof in the manufacture of a medicament for treating a tumor-related disease.

[0213] The present disclosure also provides the following synthetic methods.

[0214] Synthesis method 1: [ka]

[0215] Synthesis method 2: [ka]

[0216] The present disclosure also provides the following assay methods.

[0217] Assay method 1: KRAS G12D Assay of inhibitory activity

[0218] 1. Purpose

[0219] Compounds that can effectively inhibit the binding of KRAS to GTP were screened using the TR-FRET method.

[0220] 2. Consumables and Equipment [Table 1]

[0221] 3. Reagent Preparation

[0222] A. Stock Reagents:

[0223] 1) KRAS Nucleotide Exchange Buffer

[0224] 20 mL of 1000 mM HEPES, 500 mL of 500 mM EDTA, 10 mL of 5 M sodium chloride, 0.1 mL of 100% Tween 20, and 949.9 mL of water were weighed and combined to make 1 L of solution. The solution was sterilized by filtration and stored at 4°C.

[0225] 2) KRAS Assay Buffer

[0226] 20 mL of 1000 mM HEPES, 10 mL of 1000 mM magnesium chloride, 30 mL of 5 M sodium chloride, 0.05 mL of 100% Tween 20, and 939.95 mL of water were weighed and combined to make 1 L of solution. The solution was sterilized by filtration and stored at 4° C.

[0227] 3)KRAS / Bodipy GDP / Tb-SA mixture

[0228] 95μM KRAS G12D 9.5 μL of protein and 440.5 μL of KRAS nucleotide exchange buffer were weighed and mixed. The mixture was incubated at room temperature for 1 hour and then combined with 8.4 μL of 17.9 μM Tb-SA, 1.8 μL of 5 mM Bodipy GDP, and 9539.8 μL of KRAS assay buffer to make 1 L of solution. After mixing, the solution was left at room temperature for 6 hours and then stored at -80°C.

[0229] b. Assay Reagents:

[0230] 1) KRAS kinase solution

[0231] 73.3 μL of the KRAS / Bodipy GDP / Tb-SA mixture and 2126.7 μL of KRAS Assay Buffer were weighed and combined to make 2200 μL of solution.

[0232] 2)SOS / GTP mixture

[0233] 1.59 μL of 166 μM SOS protein, 198 μL of 100 mM GTP and 2000.41 μL of KRAS assay buffer were weighed and combined to make 2200 μL of solution.

[0234] 4. Assay Process

[0235] 1) The concentration of the stock solution of the control compound was 1 mM, and the concentration of the stock solution of the compound to be assayed was 10 mM. 9 μL of the control compound and the compound to be assayed were transferred to a 384-LDV plate.

[0236] 2) Compounds on the LDV plate were serially diluted 3-fold using a Bravo to give 10 concentrations.

[0237] 3) 9 nL of the compound on the LDV plate was transferred to the assay plate using ECHO.

[0238] 4) 3 μL of the 3 nM Kras / 0.5 nM TB-SA / 30 nM Bodipy GDP mixture and 3 μL of Ras buffer were added sequentially to each well of the assay plate using a Dragonfly autosampler, and the assay plate was centrifuged at 1000 rpm / min for 1 minute.

[0239] 5) The assay plate was incubated at room temperature for 1 hour.

[0240] 6) 3 μL of the 120 nM SOS / 9 mM GTP mixture was added to each well of the assay plate using a Dragonfly autosampler and the assay plate was centrifuged at 1000 rpm / min for 1 minute.

[0241] 7) The assay plate was incubated at room temperature for 1 hour.

[0242] 8) The plates were read using Envision and the data recorded.

[0243] 9) Data was analyzed using Excel and Xlfit to obtain IC values ​​for the compounds assayed. 50 was calculated.

[0244] Assay method 2: Assay of p-ERK inhibition in AGS cells

[0245] 1. Purpose

[0246] Compounds that can effectively inhibit p-ERK in AGS cells were screened by HTRF method.

[0247] 2. Assay Process

[0248] 1) AGS cells were seeded into a clear 96-well cell culture plate. Each well contained 80 μL of cell suspension and 10,000 cells. The cell plate was incubated at 37° C. overnight in a carbon dioxide incubator.

[0249] 2) After incubation was completed, the cell supernatant was discarded. 80 μL of medium containing 0.02% serum was added to each well. The cell plate was incubated at 37° C. overnight in a carbon dioxide incubator.

[0250] 3) 2 μL of compound was weighed and added to 78 μL of cell culture medium. After the mixture was mixed thoroughly, 20 μL of compound solution was weighed and added to the corresponding wells of the cell plate. The cell plate was placed back into the carbon dioxide incubator and incubated for an additional 3 hours.

[0251] 4) After incubation was completed, the cell supernatant was discarded. 50 μL of 1x cell lysate was added to each well. The mixture was incubated at room temperature with shaking for 30 minutes.

[0252] 5) Phospho-ERK1 / 2 Eu cryptate antibody and phospho-ERK1 / 2 d2 antibody were diluted 20-fold with detection buffer.

[0253] 6) 16 μL of cell lysate supernatant was weighed and added to each well of a new 384 white microwell plate, followed by 2 μL of a diluted solution of phospho-ERK1 / 2 Eu cryptate antibody and 2 μL of a diluted solution of phospho-ERK1 / 2 d2 antibody. The mixture was incubated at room temperature for at least 4 hours.

[0254] 7) After the incubation was completed, HTRF was read on a multi-label analyzer (excitation: 320 nm, emission: 615 nm, 665 nm).

[0255] 8) IC of the compound being assayed 50 was calculated.

[0256] Assay method 3: Assay of p-ERK inhibition in GP2D cells

[0257] 1. Purpose

[0258] Compounds that can effectively inhibit p-ERK in GP2D cells were screened by HTRF method.

[0259] 2. Assay Process

[0260] 1) GP2D cells were seeded into a transparent 96-well cell culture plate. Each well contained 80 μL of cell suspension and 8000 cells. The cell plate was incubated overnight at 37° C. in a carbon dioxide incubator.

[0261] 2) 2 μL of compound was weighed and added to 78 μL of cell culture medium. After the mixture was mixed thoroughly, 20 μL of compound solution was weighed and added to the corresponding wells of the cell plate. The cell plate was placed back into the carbon dioxide incubator and incubated for an additional hour.

[0262] 3) After incubation was completed, the cell supernatant was discarded. 50 μL of 1x cell lysate was added to each well. The mixture was incubated at room temperature with shaking for 30 minutes.

[0263] 4) Phospho-ERK1 / 2 Eu cryptate antibody and phospho-ERK1 / 2 d2 antibody were diluted 20-fold with detection buffer.

[0264] 5) 16 μL of cell lysate supernatant was weighed and added to each well of a new 384 white microwell plate, followed by 2 μL of a diluted solution of phospho-ERK1 / 2 Eu cryptate antibody and 2 μL of a diluted solution of phospho-ERK1 / 2 d2 antibody. The mixture was incubated at room temperature for at least 4 hours.

[0265] 6) After the incubation was completed, HTRF was read on a multi-label analyzer (excitation: 320 nm, emission: 615 nm, 665 nm).

[0266] 7) IC of the compound being assayed 50 was calculated.

[0267] Assay method 4: Assay of p-ERK inhibition in PANC0403 cells

[0268] 1. Assay Materials:

[0269] PANC0403 cells purchased from Nanjing Kebai; RPMI-1640 medium purchased from Biological Industries; fetal bovine serum purchased from Biosera; and Advanced phospho-ERK1 / 2 (THR202 / TYR204) kit purchased from Cisbio.

[0270] [Table 2]

[0271] 2. Assay Method:

[0272] 1) PANC0403 cells were seeded into a clear 96-well cell culture plate. Each well contained 80 μL of cell suspension and 10,000 PANC0403 cells. The cell plate was incubated overnight at 37° C. in a carbon dioxide incubator.

[0273] 2) The compounds to be assayed were diluted with 100% DMSO to 2 mM as the first concentration, and then serially diluted 5-fold using a pipette to eight concentrations (i.e., 2 mM to 25.6 nM). 2 μL of compound was weighed and added to 78 μL of cell starvation medium. After the mixture was mixed thoroughly, 20 μL of compound solution was weighed and added to the corresponding wells of the cell plate. The cell plate was placed back into the carbon dioxide incubator and incubated for an additional 3 hours. At this time, the compound concentrations were 10 μM to 0.128 nM, and the DMSO concentration was 0.5%.

[0274] 3) After incubation was completed, the cell supernatant was discarded. 50 μL of cell lysate was added to each well. The mixture was incubated at room temperature with shaking for 30 minutes.

[0275] 4) Phospho-ERK1 / 2 Eu cryptate antibody and phospho-ERK1 / 2 d2 antibody were diluted 20-fold with detection buffer.

[0276] 5) 16 μL of cell lysate supernatant was weighed and added to each well of a new 384 white microwell plate, followed by 2 μL of a diluted solution of phospho-ERK1 / 2 Eu cryptate antibody and 2 μL of a diluted solution of phospho-ERK1 / 2 d2 antibody. The mixture was incubated overnight at room temperature.

[0277] 6) After the incubation was completed, HTRF was read on a multi-label analyzer (excitation: 320 nm, emission: 615 nm, 665 nm).

[0278] 3. Data Analysis:

[0279] Raw data were converted to percentage inhibition using the formula: (sample-min) / (max-min)*100%. IC was calculated by curve fitting with four parameters (GraphPad Prism, “log(inhibitor) vs. response--Variable slope” mode). 50 The value can be obtained.

[0280] Maximum well: Positive control well reading was the 1x lysate reading.

[0281] Min Well: The reading of the negative control well was the reading of the cell lysate in the 0.5% DMSO cell well.

[0282] Assay method 5: Anti-cell proliferation effect of compounds in tumor cell lines AsPC-1 and GP2D

[0283] Test Purpose

[0284] In this assay, the effect of compounds in inhibiting cell proliferation was tested by detecting the effect of compounds on in vitro cellular activity in tumor cell lines AsPC-1 and GP2D.

[0285] Assay materials

[0286] [Table 3]

[0287] Ultra Low Cluster 96-well plate (Corning-7007)

[0288] Greiner CELLSTAR 96-well plate (#655090)

[0289] Promega CellTiter-Glo 3D Luminescent Cell Viability Assay Kit (Promega-G9683)

[0290] 2104-10 EnVision Plate Reader, PerkinElmer

[0291] RPMI 1640, DMEM, PBS (phosphate buffered saline), FBS (fetal bovine serum), antibiotic-antimycotic, L-glutamine, DMSO (dimethyl sulfoxide)

[0292] Assay Methods and Procedures

[0293] cell culture

[0294] Tumor cell lines were cultured in a 5% CO2 incubator at 37°C according to the culture conditions indicated in the culture method. Cells were passaged periodically, and cells in the logarithmic growth phase were used for plating.

[0295] Cell plating

[0296] Cells were stained with trypan blue and viable cells were counted.

[0297] The cell concentration was adjusted to the appropriate concentration (AsPC-1: 7000 cells / well; GP2D: 8000 cells / well).

[0298] 135 μL of cell suspension was added to each well of the ULA culture plate. Blank control wells received an equal volume of medium without cells.

[0299] After plating, the ULA culture plate was immediately centrifuged at 1000 rpm for 10 min at room temperature. NOTE: After centrifugation is complete, subsequent operations must be handled carefully to avoid unnecessary shocks.

[0300] The culture plates were incubated overnight in an incubator at 37°C, 5% CO2, and 100% relative humidity.

[0301] Formulation of 10x compound standard solutions and cell treatment with compounds (Day 1)

[0302] 10x compound standard solutions (10x standard solutions in DMSO) were formulated and then 15 μL of the 10x compound standard solutions were added to the ULA culture plates, and 15 μL of the DMSO cell media mixture was added to the vehicle and blank controls, respectively.

[0303] The 96-well cell plate was placed back into the incubator and incubated for 120 hours.

[0304] The cells were observed daily for sphere formation until the end of the assay.

[0305] CellTiter-Glo Luminescent Cell Viability Detection (Day 5)

[0306] The following steps were performed according to the instructions of the Promega CellTiter-Glo 3D Luminescent Cell Viability Assay Kit (Promega #G9683).

[0307] 150 μL of CellTiter-Glo 3D Reagent (equal to the cell culture medium volume in each well) was added to each well. The cell plates were wrapped in aluminum foil to protect from light.

[0308] The culture plate was shaken on an orbital shaker for 5 minutes.

[0309] To ensure that the cell spheroids were adequately detached before proceeding to the next step, the mixture in the well was thoroughly mixed by carefully pipetting up and down 10 times with a pipette.

[0310] The solution in the ULA plate was then transferred to a black bottom culture plate (#655090) and left at room temperature for 25 minutes to allow the luminescence signal to stabilize.

[0311] Luminescent signals were detected with a 2104 EnVision plate reader.

[0312] Data analysis

[0313] The IR of the assay compounds was calculated using the formula: Inhibition percentage (IR) (%) = (1-(RLU of compound-RLU of blank control) / (RLU of vehicle control-RLU of blank control))*100%. The inhibition percentages of different concentrations of compounds were calculated in Excel, and then inhibition curves were generated using GraphPad Prism software to record the minimum inhibition percentage, maximum inhibition percentage and IC 50 The relevant parameters were calculated, including:

[0314] Assay method 6: Pharmacokinetic testing of oral and intravenous injection assay compounds in CD-1 mice

[0315] Assay Purpose

[0316] To assay the in vivo pharmacokinetics of orally and intravenously injected compounds in CD-1 mice.

[0317] Assay process

[0318] The assay compounds were mixed with 5% DMSO + 95% (10% HP-β-CD) aqueous solution. The mixture was vortexed and sonicated to prepare a clear solution of 0.5 mg / mL (intravenous) or 3 mg / mL (oral). For use, the clear solution was filtered through a microporous membrane. Male SD mice aged 7 to 10 weeks were selected and administered the candidate compound solution intravenously at a dose of approximately 2 mg / kg and orally at a dose of approximately 30 mg / kg. Whole blood was collected at a certain period and prepared to obtain plasma. Drug concentrations were analyzed by LC-MS / MS method, and pharmacokinetic parameters were calculated with Phoenix WinNonlin software (Pharsight, USA).

[0319] Assay method 7: Plasma protein binding (PPB) assay

[0320] Assay Objective

[0321] Protein binding rates of the compounds in plasma of CD-1 mice, Sprague-Dawley rats, beagle dogs, cynomolgus monkeys and humans were determined by equilibrium dialysis.

[0322] Assay Method

[0323] The above five types of plasma were combined into plasma samples with a compound concentration of 2 μM. The plasma samples were placed in a 96-well rapid equilibrium dialysis device and dialyzed with phosphate-buffered saline at 37 ± 1 °C for 4 hours. Warfarin was used as a control compound in this assay. The concentrations of the analytes in the plasma and dialysis buffer were determined by LC-MS / MS method.

[0324] Assay method 8: Assay of p-ERK inhibition in AsPC-1 cells

[0325] 1. Purpose

[0326] The inhibitory activity of the compounds on p-ERK levels in AsPC-1 cells was evaluated by HTRF method.

[0327] 2. Assay Materials:

[0328] ASPC-1 cells purchased from ATCC; RPMI-1640 medium purchased from Gibco; fetal bovine serum purchased from Hyclone; and Advanced phospho-ERK1 / 2 (THR202 / TYR204) kit purchased from Bioauxilium.

[0329] [Table 4]

[0330] 3. Assay Method:

[0331] 1) ASPC-1 cells were seeded into 384-well cell culture plates with white bottoms at 8 μL of cell suspension per well. Each well contained 7500 cells. The cell plates were incubated overnight at 37° C. in a carbon dioxide incubator.

[0332] 2) The compounds to be assayed were diluted to 3 mM as the first concentration with 100% DMSO, then diluted to 3000 μM, 1000 μM, 300 μM, 100 μM, 30 μM, 10 μM, 3 μM, 1 μM, 0.3 μM, and 0.1 μM using a pipette. 2 μL of compound was weighed and added to 198 μL of cell starvation medium. After the mixture was mixed thoroughly, 15 μL of compound solution was weighed and added to 35 μL of cell starvation medium. The mixture was mixed thoroughly. The compound solution from the final step was then added to the corresponding cell plate wells at 4 μL per well. The cell plate was placed back into the carbon dioxide incubator and incubated for an additional 3 hours. At this time, the concentrations of the compounds were 3000 nM, 1000 nM, 300 nM, 100 nM, 30 nM, 10 nM, 3 nM, 1 nM, 0.3 nM and 0.1 nM.

[0333] 3) After the incubation was completed, 3 μL of 5x cell lysate was added to each well. The mixture was incubated at room temperature with shaking for 30 minutes.

[0334] 4) The phospho-ERK1 / 2 Eu cryptate antibody and the phospho-ERK1 / 2 d2 antibody were diluted 20-fold in detection buffer and mixed thoroughly in a 1:1 ratio. The mixture was added to the cell culture plate at 5 μL per well and incubated at room temperature for 2 hours.

[0335] 5) After the incubation was completed, HTRF was read on a multi-label analyzer (excitation: 320 nm, emission: 615 nm, 665 nm).

[0336] 4. Data Analysis:

[0337] Raw data were converted to percentage inhibition using the formula: (sample-min) / (max-min)*100%. IC was calculated by curve fitting with four parameters (GraphPad Prism, “log(inhibitor) vs. response--Variable slope” mode). 50 The value can be obtained.

[0338] Maximum well: Positive control well reading was the 1x lysate reading.

[0339] Min Well: The reading of the negative control well was the reading of the cell lysate in the 0.5% DMSO cell well.

[0340] Related Definitions

[0341] Unless otherwise specified, the following terms and phrases used herein are intended to have the following meanings: Certain terms or phrases should not be considered unclear or indefinite in the absence of a specific definition, but should be understood in their conventional sense. When a trade name appears herein, it is intended to refer to the corresponding product or its active ingredients.

[0342] The term "pharmacologically acceptable" is used herein to refer to compounds, materials, compositions and / or dosage forms that are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals and are free of excessive toxicity, irritation, allergic response, or other problem or complication and are commensurate with a reasonable benefit / risk ratio.

[0343] The term "pharmaceutically acceptable salt" refers to a salt of a compound disclosed herein prepared by reacting a compound having certain substituents disclosed herein with a relatively non-toxic acid or base. When a compound disclosed herein contains a relatively acidic functional group, a base addition salt can be obtained by contacting the compound with a sufficient amount of base in a pure solution or in a suitable inert solvent. Pharmaceutically acceptable base addition salts include sodium, potassium, calcium, ammonium, organic amine or magnesium salts, or similar salts. When a compound disclosed herein contains a relatively basic functional group, an acid addition salt can be obtained by contacting the compound with a sufficient amount of acid in a pure solution or in a suitable inert solvent. Certain compounds disclosed herein contain both basic and acidic functional groups and can be converted to any base or acid addition salt.

[0344] The pharma- ceutically acceptable salts disclosed herein can be prepared from a parent compound that contains an acidic or basic moiety by conventional chemical methods. Generally, such salts can be prepared by reacting the free acid or base form of the compound with a stoichiometric amount of the appropriate base or acid in water or an organic solvent, or a mixture thereof.

[0345] The compounds disclosed herein may exist in specific geometric or stereoisomeric forms. The present disclosure contemplates all such compounds, including cis and trans isomers, (-) and (+) enantiomers, (R) and (S) enantiomers, diastereoisomers, (D) isomers, (L) isomers, and racemic and other mixtures, such as mixtures enriched in enantiomers or diastereoisomers, all of which are included within the scope disclosed herein. Substituents such as alkyl may have additional asymmetric carbon atoms. All of these isomers and mixtures thereof are included within the scope disclosed herein.

[0346] The compounds disclosed herein may contain unnatural proportions of atomic isotopes at one or more of the atoms that constitute the compounds. For example, the compounds may contain tritium ( 3 H), iodine-125( 125 I) or C-14( 14 For another example, hydrogen can be replaced with deuterium to form a deuterated drug. The bond between deuterium and carbon is stronger than the bond between normal hydrogen and carbon. Compared to non-deuterated drugs, deuterated drugs have the advantages of reduced toxic side effects, increased drug stability, improved efficacy, and increased biological half-life of the drug. Any change in the isotopic composition of the compounds disclosed herein, regardless of radioactivity, is included within the scope of this disclosure.

[0347] The term "optional" or "optionally" means that a subsequent event or condition may, but need not, occur, and the term includes instances where the event or condition occurs as well as instances where the event or condition does not occur.

[0348] The term "substituted" means that one or more atoms on a particular atom are replaced by a substituent, including deuterium and hydrogen variants, so long as the valence of the particular atom is normal and the substituted compound is stable. If the substituent is oxo (i.e., =O), it means that two hydrogen atoms are replaced. A position on an aromatic ring cannot be replaced by oxo. The term "optionally substituted" means that an atom may or may not be substituted by a substituent, and unless otherwise specified, the type and number of substituents can be any as long as it is chemically achievable.

[0349] When any variable (such as R) occurs more than one time in a constituent or structure of a compound, the definition of that variable at each occurrence is independent. So, for example, if a group is substituted with 0-2 R, the group may be optionally substituted with up to 2 R, but the definition of R at each occurrence is independent. Also, combinations of substituents and / or variables thereof are permissible only if such combinations result in stable compounds.

[0350] When the number of linking groups is 0, such as -(CRR)0-, it means that the linking group is a single bond.

[0351] When one of the variables is a single bond, it means that the two groups linked by the single bond are directly connected. For example, if L in ALZ represents a single bond, then the structure of ALZ is actually AZ.

[0352] If the listed linking group does not indicate the direction of the link, then the bond may be oriented in any direction. For example, [ka] When the linking group L in is -MW-, -MW is linked to ring A and ring B in the same direction as the reading order from left to right. [ka] or may be connected to ring A and ring B in the reverse left-to-right reading order. [ka] Combinations of linking groups, substituents and / or variables thereof are permissible only if such combinations result in stable compounds.

[0353] Unless otherwise specified, when a group has one or more connectable sites, any one or more sites of the group can be connected to other groups via a chemical bond. When the connection position of the chemical bond is variable, if there is an H atom at the connectable site and the connectable site having an H atom is connected to a chemical bond, the number of H atoms at this site decreases accordingly as the number of connected chemical bonds increases, and the group becomes a group of the corresponding valency. The chemical bond between the site and other groups is represented by a solid straight bond ( [ka] ), dashed straight line bond ( [ka] ) or wavy line ( [ka] For example, a solid straight bond in -OCH3 indicates that the group is connected to another group through the oxygen atom therein; [ka] a dashed straight bond in indicates that the group is connected to another group through two ends of the nitrogen atom therein; [ka] The wavy lines in the middle indicate that the group is connected to other groups via the 1- and 2-carbon atoms in the phenyl group, [ka] Any possible attachment site on the piperidinyl group has at least four attachment methods. [ka] Even though an H atom is drawn on the -N-, it indicates that the group can be connected to other groups through one chemical bond, etc. [ka] is still [ka] This simply means that when one chemical bond is connected, this site will have only one less H and the group will become the corresponding monovalent piperidinyl group.

[0354] Unless otherwise stated, solid wedge connections ( [ka] ) and the dashed wedge bond ( [ka] ) indicates the absolute configuration of the stereocenter, and a solid linear bond ( [ka] ) and dashed straight line bond ( [ka] ) indicates the relative configuration of stereocenters, and the wavy line ( [ka] ) is a solid wedge bond ( [ka] ) or dashed wedge bond ( [ka] ) or a wavy line ( [ka] ) is a solid straight line combination ( [ka] ) or dashed straight bond ( [ka] ) is shown.

[0355] Unless otherwise stated, when double bond structures such as carbon-carbon double bonds, carbon-nitrogen double bonds, and nitrogen-nitrogen double bonds are present in a compound and each atom on the double bond is connected to two different substituents (in double bonds involving nitrogen atoms, the lone electron pairs on the nitrogen atoms are considered as the substituents connected to them), the atoms on the double bonds in the compound and their substituents are [ka] represents the (Z)-isomer, (E)-isomer or a mixture of both isomers of a compound. Unless otherwise specified, the term "tautomer" or "tautomeric form" means that at room temperature, each isomer with different functional groups is in dynamic equilibrium and can be rapidly converted into one another. When tautomerism is possible (such as in solution), a chemical equilibrium of tautomerism can be achieved. For example, proton tautomers (prototropic tautomers) include interconversions via proton transfer, such as keto-enol isomerization and imine-enamine isomerization. Valence isomers are tautomers that interconvert by rearrangement of some of the bond electrons. A specific example of keto-enol tautomerization is the interconversion of pentane-2,4-dione and 4-hydroxypent-3-en-2-one.

[0356] The terms "halo" or "halogen," by themselves or as part of another substituent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom.

[0357] Unless otherwise stated, "C1-3 The term "alkyl" is used to represent a linear or branched saturated hydrocarbon group consisting of 1 to 3 carbon atoms. 1-3 Alkyl is C 1-2 Alkyl, C 2-3 It may be monovalent (such as methyl), divalent (such as methylene) or polyvalent (such as methenyl). 1-3 Examples of alkyl include, but are not limited to, methyl (Me), ethyl (Et), propyl (including n-propyl and isopropyl), and the like.

[0358] Unless otherwise stated, "C 1-3 "Alkoxy" means an alkyl group containing 1 to 3 carbon atoms and attached to the remainder of the molecule by an oxygen atom. 1-3 The alkoxy group is C 1-2 , C 2-3 , C3 and C2 alkoxy groups, etc. 1-3 Examples of alkoxy groups include, but are not limited to, methoxy, ethoxy, propoxy (including n-propoxy and isopropoxy), and the like.

[0359] Unless otherwise stated, "C 2-3 "Alkenyl" is used to represent a linear or branched hydrocarbon group of 2 to 3 carbon atoms containing at least one carbon-carbon double bond, which may be located at any position of the group. 2-3 Alkenyl includes C3 and C2 alkenyl. 2-3 Alkenyl may be monovalent, divalent or polyvalent. 2-3 Examples of alkenyl include, but are not limited to, vinyl, propenyl, and the like.

[0360] Unless otherwise stated, "C 2-3 "Alkynyl" is used to represent a linear or branched hydrocarbon group of 2 to 3 carbon atoms containing at least one carbon-carbon triple bond, which may be located at any position of the group. 2-3Alkynyl may be monovalent, divalent or polyvalent. 2-3 Alkynyl includes C3 and C2 alkynyl. 2-3 Examples of alkynyl include, but are not limited to, ethynyl, propynyl, and the like.

[0361] Unless otherwise stated, "C 2-4 "Alkynyl" is used to represent a linear or branched hydrocarbon group of 2 to 4 carbon atoms containing at least one carbon-carbon triple bond, which may be located at any position of the group. 2-4 The alkynyl group is C 2-3 , C4, C3 and C2 alkynyl groups. 2-4 Alkynyl may be monovalent, divalent or polyvalent. 2-4 Examples of alkynyl groups include, but are not limited to, ethynyl, propynyl, butynyl, and the like.

[0362] Unless otherwise stated, "C 3-5 "Cycloalkyl" refers to a monocyclic, saturated cyclic hydrocarbon group of 3 to 5 carbon atoms. 3-5 Cycloalkyl is C 3-4 and C 4-5 C includes cycloalkyl, and may be monovalent, divalent or polyvalent. 3-5 Examples of cycloalkyl include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, and the like.

[0363] Unless otherwise specified, the terms "5- to 6-membered heteroaromatic ring" and "5- to 6-membered heteroaryl" may be used interchangeably herein. The term "5- to 6-membered heteroaryl" refers to a monocyclic group having a conjugated pi-electron system and consisting of 5 to 6 ring atoms, in which 1, 2, 3, or 4 ring atoms are heteroatoms independently selected from O, S, and N, and the remainder are carbon atoms. The nitrogen atom is optionally quaternized, and the nitrogen and sulfur heteroatoms are optionally oxidized (i.e., NO and S(O)). p(p is 1 or 2). The 5-6 membered heteroaryl group may be attached to the remainder of the molecule via a heteroatom or a carbon atom. The group may be monovalent, divalent or polyvalent. The 5-6 membered heteroaryl group includes 5-membered and 6-membered heteroaryl groups. Examples of 5-6 membered heteroaryls include pyrrolyl (including N-pyrrolyl, 2-pyrrolyl and 3-pyrrolyl, etc.), pyrazolyl (including 2-pyrazolyl and 3-pyrazolyl, etc.), imidazolyl (including N-imidazolyl, 2-imidazolyl, 4-imidazolyl and 5-imidazolyl, etc.), oxazolyl (including 2-oxazolyl, 4-oxazolyl and 5-oxazolyl, etc.), triazolyl (including 1H-1,2,3-triazolyl, 2H-1,2,3-triazolyl, 1H-1,2,4-triazolyl and 4H-1,2,4-triazolyl, etc.), and the like. triazolyl, tetrazolyl, isoxazolyl (including 3-isoxazolyl, 4-isoxazolyl, and 5-isoxazolyl), thiazolyl (including 2-thiazolyl, 4-thiazolyl, and 5-thiazolyl, etc.), furyl (including 2-furyl and 3-furyl, etc.), thienyl (including 2-thienyl, 3-thienyl, etc.), pyridyl (including 2-pyridyl, 3-pyridyl, and 4-pyridyl, etc.), pyrazinyl, or pyrimidinyl (including 2-pyrimidinyl, 4-pyrimidinyl, etc.).

[0364] Unless otherwise specified, the terms "5-10 membered heteroaromatic ring" and "5-10 membered heteroaryl" may be used interchangeably. The term "5-10 membered heteroaryl" refers to a cyclic group having a conjugated pi electron system and consisting of 5-10 ring atoms, in which 1, 2, 3, or 4 ring atoms are heteroatoms independently selected from O, S, and N, and the remainder are carbon atoms. The group may be a monocyclic, fused bicyclic, or fused tricyclic ring system, in which each ring is aromatic, the nitrogen atom is optionally quaternized, and the nitrogen and sulfur heteroatoms are optionally oxidized (i.e., NO and S(O)). p(p is 1 or 2). The 5-10 membered heteroaryl can be attached to the remainder of the molecule via a heteroatom or a carbon atom. The group can be monovalent, divalent or polyvalent. The 5-10 membered heteroaryl group includes 5-8 membered, 5-7 membered, 5-6 membered, 5 membered and 6 membered heteroaryl groups. Examples of 5-10 membered heteroaryls include pyrrolyl (including N-pyrrolyl, 2-pyrrolyl, 3-pyrrolyl, etc.), pyrazolyl (including 2-pyrazolyl and 3-pyrazolyl, etc.), imidazolyl (including N-imidazolyl, 2-imidazolyl, 4-imidazolyl and 5-imidazolyl, etc.), oxazolyl (including 2-oxazolyl, 4-oxazolyl and 5-oxazolyl, etc.), triazolyl (1H-1,2,3-triazolyl, 2H-1,2,3-triazolyl, 1H-1,2,4-triazolyl and 4H-1,2,4-triazolyl, etc.), tetrazolyl, isoxazolyl (3-isoxazolyl, 4-isoxazolyl and 5-isoxazolyl, etc.), thiazolyl (2-thiazolyl, 4-thiazolyl and 5-thiazolyl, etc.). and the like), furyl (including 2-furyl and 3-furyl, and the like), thienyl (including 2-thienyl and 3-thienyl, and the like), pyridyl (including 2-pyridyl, 3-pyridyl, and 4-pyridyl, and the like), pyrazinyl or pyrimidinyl (including 2-pyrimidinyl and 4-pyrimidinyl, and the like), benzothiazolyl (including 5-benzothiazolyl, and the like), purinyl, benzimidazolyl (including 2-benzimidazolyl, and the like), benzoxazolyl, indolyl (including 5-indolyl, and the like), isoquinolyl (including 1-isoquinolyl, 5-isoquinolyl, and the like), quinoxalinyl (including 2-quinoxalinyl, 5-quinoxalinyl, and the like) or quinolyl (including 3-quinolyl, 6-quinolyl, and the like).

[0365] Unless otherwise stated, C n-n+m Or C n -C n+m includes any particular case of n to n+m carbons. For example, C 1-12 are C1, C2, C3, C4, C5, C6, C7, C8, C9, C 10 , C 11 and C 12and further including any range of n to n+m, for example, C 1-12 is C 1-3 , C 1-6 , C 1-9 , C 3-6 , C 3-9 , C 3-12 , C 6-9 , C 6-12 and C 9-12 Similarly, n-membered to n+m-membered rings refer to the number of atoms on the ring being n to n+m, and for example, a 3- to 12-membered ring includes a 3-membered ring, a 4-membered ring, a 5-membered ring, a 6-membered ring, a 7-membered ring, an 8-membered ring, a 9-membered ring, a 10-membered ring, an 11-membered ring, and a 12-membered ring, and further includes any range of n to n+m, and for example, a 3- to 12-membered ring includes a 3- to 6-membered ring, a 3- to 9-membered ring, a 5- to 6-membered ring, a 5- to 7-membered ring, a 6- to 7-membered ring, a 6- to 8-membered ring, and a 6- to 10-membered ring, etc.

[0366] The compounds disclosed herein can be prepared by a variety of synthetic methods known to those of skill in the art, including the embodiments enumerated below, embodiments formed by the embodiments enumerated below in combination with other chemical synthetic methods, and equivalent alternatives known to those of skill in the art, including, but not limited to, the embodiments disclosed herein.

[0367] The structure of the compounds disclosed herein can be confirmed by conventional methods well known to those skilled in the art.When the present disclosure relates to the absolute configuration of a compound, the absolute configuration can be confirmed by conventional techniques in the art, such as single crystal X-ray diffraction (SXRD).In single crystal X-ray diffraction (SXRD), a Bruker D8 Venture diffractometer equipped with a CuKα source in φ / ω scanning mode is used to collect the diffraction intensity data of the cultured single crystal, and after collecting the data, the crystal structure is further analyzed by direct method (Shelx97) to confirm the absolute configuration.

[0368] The solvents used in this disclosure are commercially available. This disclosure employs the following abbreviations: PhNTf2 represents N-phenylbis(trifluoromethanesulfonyl)imide; Pd2(dba)3 represents tris[dibenzylideneacetone]dipalladium; DavePhos represents 2-dicyclohexylphosphino-2'-(N,N-dimethylamino)-biphenyl; LIHMDS represents lithium bis(trimethylsilyl)amide; NaOH represents sodium hydroxide; NaHCO3 represents sodium bicarbonate; Boc2O represents di-tert-butyl dicarbonate; Et3N represents triethylamine; Tf2O represents trifluoroacetic anhydride; Xantphos represents 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene; DMF represents N,N-dimethylformamide. DMAP represents 4-dimethylaminopyridine; TBDPSCl represents tert-butyldiphenylchlorosilane; DCM represents dichloromethane; EA represents ethyl acetate; Grubbs II catalyst represents 1,3-bis(2,4,6-trimethylphenyl)-2-(imidazolidinylidene)(dichlorophenylmethylene)(tricyclohexylphosphine)ruthenium; TFA represents trifluoroacetic acid; THF represents tetrahydrofuran; NBS represents N-bromosuccinimide; PMB represents p-methoxybenzyl; Tf represents trifluoromethanesulfonyl; Boc represents tert-butyloxycarbonyl; TIPS represents triisopropylsilyl; and MOM represents methoxymethyl.

[0369] Compounds are named according to common naming conventions in the art or by ChemDraw® software; commercially available compounds are named by their vendor's directory name. [Brief description of the drawings]

[0370] [Figure 1] 1 shows the binding pattern diagram of compound A and KRASG12D protein.

[0371] [Diagram 2] 1 shows the binding pattern diagram of compound B and KRASG12D protein.

[0372] [Diagram 3] 1 shows the binding pattern diagram of compound C and KRASG12D protein.

[0373] [Figure 4] 1 shows the binding pattern diagram of compound D and KRASG12D protein.

[0374] [Diagram 5] 1 shows the binding pattern diagram of compound E and KRASG12D protein.

[0375] [Figure 6] 1 shows the binding pattern diagram of compound F and KRASG12D protein.

[0376] Technical effects

[0377] The compounds of the present disclosure inhibit KRAS G12D It has a good inhibitory effect on mutant enzymes and can effectively inhibit p-ERK and KRAS G12D The compounds of the present disclosure have good cell proliferation inhibitory activity against mutant cells, can effectively inhibit tumor growth in vivo, and have good drug tolerance. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0378] The present disclosure will be described in detail below with reference to examples. However, these examples are not intended to have any adverse limitations on the present disclosure. The present disclosure has been described in detail herein, and embodiments are also disclosed herein. It will be apparent to those skilled in the art that various changes and modifications can be made to the embodiments disclosed herein without departing from the spirit and scope disclosed herein.

[0379] Calculation Example 1 [ka]

[0380] The present disclosure also provides a docking process of a compound or its pharma- ceutically acceptable molecule, which is performed by using the default options of Glide SP [1] and Maestro (Schrodinger version 2017-2). The crystal structure PDB:6UT0 of KRAS_G12C in the PDB database was selected. Cys12 was mimetic mutated to Asp12, which was used as a docking template after energy optimization. To prepare the protein, hydrogen atoms were added using the Protein Preparation Wizard module of Maestro [2], and the OPLS3 force field was used. To prepare the ligand, the three-dimensional structure of the molecule was created using LigPrep and energy minimized [3]. The small molecule conformation was sampled using the confgen module. A cubic docking grid with edge lengths of 25 Å*25 Å*25 Å was created using the ligand of 6UT0 as the center of mass. The reference compound was placed during the molecular docking process. After analyzing the interaction type between the protein receptor and the ligand, reasonable docking conformations were selected and stored based on the calculated docking scores and binding modes, as shown in Figures 1 to 6.

[0381] [1] Glide, Schrodinger, LLC, New York, NY, 2017

[0382] [2]Maestro, Schrodinger, LLC, New York, NY, 2017

[0383] [3]LigPrep, Schrodinger, LLC, New York, NY, 2017

[0384] Conclusion: The compounds disclosed herein inhibit KRAS G12D It binds well to

[0385] Example 1 [ka] Step 1: Synthesis of intermediate 1-2

[0386] Compound 1-1 (20 g, 137.40 mmoL), N,N-dimethylformamide (200 mL), potassium iodide (22.81 g, 137.40 mmoL) and anhydrous potassium carbonate (47.47 g, 343.50 mmoL) were added to a pre-dried reaction flask. P-Methoxybenzyl chloride (44.11 g, 281.67 mmol, 38.36 mL) was added with stirring, and then the mixture was heated to 65° C. and stirred for 4 hours. The reaction solution was cooled to room temperature and then filtered through a Celite® pad. The filter cake was rinsed with 200 mL of methyl tert-butyl ether, and then 200 mL of water was added to the filtrate for extraction. The aqueous phase was extracted with ethyl acetate (100 mL*2). The organic phases were combined, washed with saturated brine (200 mL*3), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to give the crude product. The crude product was slurried with 50 mL of petroleum ether for 48 hours. The mixture was filtered. The filter cake was collected and dried to give the product, compound 1-2, which was used directly in the next step. 1 H NMR(400MHz,DMSO-d6)δ=7.18-7.17(m,5H),6.85-6.82(m,6H),4.24(s,4H),3.74-3.71(m,6H).MS m / z=386.1[M+H] + .

[0387] Step 2: Synthesis of intermediate 1-3

[0388] 2,2,6,6-Tetramethylpiperidine (43.93 g, 311.00 mmol, 52.80 mL) was dissolved in tetrahydrofuran (300 mL) and the mixture was cooled to -5°C. n-Butyllithium (2.5 M, 124.40 mL) was added. The mixture was stirred for 0.5 h and then cooled to -60°C. A solution of compound 1-2 (30 g, 77.75 mmol) in tetrahydrofuran (30 mL) was added. The mixture was stirred for 0.5 h and then N,N-dimethylformamide (113.66 g, 1.55 mol, 119.64 mL) was added. The mixture was stirred for an additional 0.5 h. 200 mL of saturated ammonium chloride was added to the reaction solution. The mixture was extracted with ethyl acetate (200 mL). The layers were separated. The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The residue was purified by column (petroleum ether:ethyl acetate=10:1) to give compound 1-3.

[0389] Step 3: Synthesis of intermediate 1-4

[0390] Sodium hydride (6.38g, 159.47mmol, content 60%) was dissolved in tetrahydrofuran (300mL). The atmosphere was replaced with nitrogen twice, and the mixture was cooled to 0℃. Methyl acetoacetate (18.52g, 159.47mmol, 17.15mL) was added. The mixture was stirred for 10 minutes, and then n-butyllithium (2.5M, 63.79mL) was added. The mixture was stirred for another 10 minutes and cooled to -15℃. A solution of compound 1-3 (30g, 72.49mmol) in tetrahydrofuran (50mL) was added. The mixture was stirred for another 30 minutes. 100mL of saturated ammonium chloride was added to the reaction solution. The mixture was extracted with ethyl acetate (100mL*2). The organic phases were combined, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The residue was purified by column (petroleum ether:ethyl acetate=10:1) to give compound 1-4. MS m / z=530.2[M+H] + .

[0391] Step 4: Synthesis of intermediate 1-5

[0392] Compound 1-4 (20 g, 37.74 mmol) was dissolved in anhydrous dichloromethane (50 mL), N,N-dimethylformamide dimethyl acetal (5.40 g, 45.28 mmol, 6.02 mL) was added under nitrogen, and the mixture was reacted at 25°C for 16 hours. Boron trifluoride diethyl etherate (6.43 g, 45.28 mmol, 5.59 mL) was added, and the mixture was reacted at 20°C for 1 hour. The reaction solution was added to 50 mL of saturated sodium bicarbonate solution. The mixture was extracted with dichloromethane (20 mL*2). The layers were separated. The organic phases were combined, washed with 30 mL of saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1-1:1) to give compound 1-5. 1 H NMR(400MHz,CDCl3)δ=8.45(s,1H),7.14(m,4H),7.00-6.69(m,6H),5.80(m, 1H),4.27-4.10(m,5H),3.79(m,1H),3.94-3.66(m,8H),2.98-2.73(m,1H).MS m / z=540.2[M+H] + .

[0393] Step 5: Synthesis of intermediate 1-6

[0394] Compound 1-5 (12 g, 22.22 mmol) was added to anhydrous tetrahydrofuran (30 mL), tri-sec-butyllithium borohydride (11.83 g, 62.22 mmol, 13.60 mL) was added under nitrogen, and the mixture was reacted at -60°C for 1 hour. The reaction solution was added to 40 mL of water. The mixture was extracted with ethyl acetate (20 mL*2). The organic phases were combined, then washed with 20 mL of saturated brine, dried over anhydrous sodium sulfate, and concentrated. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=100:0-3:1) to give compound 1-6. MS m / z=542.2[M+H] + .

[0395] Step 6: Synthesis of intermediate 1-7

[0396] Compound 1-6 (5.5 g, 10.15 mmol) was added to ethanol (15 mL) and water (3 mL), and sodium bicarbonate (2.15 g, 20.30 mmol) and methylisothiourea sulfate (2.74 g, 30.44 mmol) were added. The mixture was reacted at 45-50°C for 16 hours. The reaction solution was extracted with water (20 mL) and ethyl acetate (20 mL*2). The combined organic phase was washed with saturated brine (20 mL*2), dried over anhydrous sodium sulfate, and concentrated to give compound 1-7. The crude product was used directly for the next step. MS m / z=582.2[M+H] + .

[0397] Step 7: Synthesis of intermediates 1-8

[0398] Compound 1-7 (6 g, 8.04 mmol) was added to anhydrous dichloromethane (20 mL). N,N-diisopropylethylamine (3.12 g, 24.12 mmol, 4.20 mL) was added at 0°C. The mixture was cooled to 0-10°C. Trifluoromethanesulfonic anhydride (4.08 g, 14.47 mmol, 2.39 mL) was slowly added dropwise. The mixture was reacted at 0°C for 0.5 h. The reaction solution was added to 20 mL of saturated ammonium chloride. The mixture was extracted with anhydrous dichloromethane (10 mL*2). The organic phases were combined, washed with 20 mL of saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=1:0-0:1) to give compound 1-8. MS m / z=714.1[M+H] + .

[0399] Step 8: Synthesis of intermediate 1-9 hydrochloride

[0400] Compound 1-8 (0.8 g, 1.12 mmoL), compound 1-1A (475.62 mg, 2.24 mmoL) and DIPEA (434.33 mg, 3.36 mmoL, 585.35 μL) were added to N,N-dimethylformamide (5 mL). The mixture was reacted at 50° C. for 1 hour. The reaction solution was added to saturated ammonium chloride (20 mL). The mixture was extracted with ethyl acetate (20 mL*2). The organic phases were combined, washed with 20 mL of saturated brine, dried over anhydrous sodium sulfate and concentrated. The crude product was purified by column (petroleum ether: ethyl acetate = 3:1) and then separated by preparative HPLC (chromatography column: Phenomenex Luna C18 250 * 50mm * 10μm; mobile phase: [water (0.05% hydrochloric acid) - acetonitrile]; (acetonitrile) %: 65% - 95%, 10 min) to obtain compound 1-9 hydrochloride. MS m / z = 776.3 [M + H] + .

[0401] Step 9: Synthesis of intermediates 1-10

[0402] Compound 1-9 hydrochloride (1.2 g) was dissolved in N,N-dimethylformamide (5 mL). The mixture was cooled to 0°C, N-bromosuccinimide (330.13 mg, 1.85 mmol) was added, and the mixture was stirred at 20°C for 1 hour. 30 mL of water was added to the reaction solution. The mixture was extracted with ethyl acetate (30 mL*2). The organic phases were combined, washed with saturated brine (20 mL*2), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The residue was purified by column (petroleum ether:ethyl acetate=3:1-1:1) to give compound 1-10. MS m / z=856.1[M+H] + .

[0403] Step 10: Synthesis of intermediates 1-11

[0404] Compound 1-10 (0.3 g, 350.77 μmol), copper(I) oxide (133.61 mg, 701.55 μmol) and methyl fluorosulfonyldifluoroacetate (336.94 mg, 1.75 mmol, 223.14 μL) were dissolved in N,N-dimethylformamide (10 mL) and the mixture was stirred at 100° C. for 1 h under nitrogen. The reaction solutions were combined and poured into 30 mL of water. The mixture was extracted with methyl tert-butyl ether (20 mL*2). The organic phases were combined, washed with saturated brine (20 mL*2), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated. The crude product was purified by column (petroleum ether:ethyl acetate=10:1-1:1) to give compound 1-11. MS m / z=844.2[M+H] + .

[0405] Step 11: Synthesis of intermediates 1-12

[0406] Compound 1-11 (0.35 g, 414.52 μmol) was added to the reaction flask. Anhydrous dichloromethane (5 mL) was added and the mixture was stirred. Then metachloroperbenzoic acid (126.24 mg, 621.78 μmol, content 85%) was added and the mixture was reacted at 25 °C for 1 hour. 5 mL of 5% sodium thiosulfate solution was added to the reaction solution. The mixture was extracted with dichloromethane (10 mL * 2). The organic phases were combined, washed with saturated brine (5 mL * 2), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The crude product was purified by column (petroleum ether: ethyl acetate = 10: 1-1: 1) to obtain compound 1-12. MS m / z = 860.2 [M + H] + .

[0407] Step 12: Synthesis of intermediate 1-13

[0408] Compound 1-2A (256.28 mg, 1.61 mmol) was added to anhydrous tetrahydrofuran (5 mL), and sodium tert-butoxide (123.76 mg, 1.29 mmol) was added. The mixture was reacted at -15°C for 15 minutes. Compound 1-12 (0.277 g, 321.96 μmol) was added, and the mixture was reacted at -15°C for 1 hour. 5 mL of saturated ammonium chloride was added to the reaction solution, and the mixture was extracted with ethyl acetate (10 mL * 3). The organic phases were combined, washed with saturated brine (20 mL * 2), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The crude product was purified by column (dichloromethane:methanol = 20: 1-10: 1) to obtain compound 1-13. MS m / z = 955.3 [M + H] + .

[0409] Step 13: Synthesis of Compounds 1A and 1B

[0410] Compound 1-13 (0.450 g, 470.98 μmol) was added to trifluoroacetic acid (2.85 g, 24.96 mmol, 1.85 mL) and dichloromethane (9 mL). The mixture was reacted at -10 to 0 °C for 1 hour. The reaction solution was poured into 10 mL of water. The lower dichloromethane phase was separated. The aqueous phase was adjusted to pH 8 to 10 with saturated sodium bicarbonate solution. The mixture was then extracted with ethyl acetate (5 mL * 2). The organic phases were combined, washed with saturated brine (5 mL * 2), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The crude product was purified by HPLC (chromatographic column: Phenomenex C18 75*30mm*3μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; (acetonitrile)%: 1%-35%, 8 min), and then separated by SFC (chromatographic column: DAICEL CHIRALCEL OD (250mm*30mm, 10μm); mobile phase: [supercritical CO2-methanol (0.1% ammonia)]; methanol (0.1% ammonia)%: 36%-36%, 7 min) to obtain compound 1A and compound 1B. Chiral SFC analysis (chromatographic column: DAICEL CHIRALCEL OD-3 (150mm*4.6mm, 3μm); mobile phase: [supercritical CO2-methanol (0.1% diethylamine)]; (methanol)%: 40%-40%), Compound 1A (Rt=3.151min, ee value 99%); Compound 1B (Rt=4.085min, ee value 93%).

[0411] Compound 1A: 1 H NMR(400MHz,CD3OD)δ=6.93(d,J=8.0Hz,1H),5.68-5.46(m,1H),5.32-5.19(m,1H), 4.78-4.69(m,1H),4.63-4.46(m,2H),4.41-4.28(m,1H),4.24-4.08(m,2H),4.04-3 .74(m,5H),3.73-3.65(m,1H),3.55-3.41(m,2H),3.02-2.89(m,1H),2.80-2.48(m, 2H),2.48-2.24(m,4H),2.24-2.04(m,3H),2.03-1.91(m,1H),2.04-1.90(m,1H).MS m / z=615.4[M+H]+ .

[0412] Compound 1B: 1 H NMR(400MHz,CD3OD)δ=6.98-6.85(m,1H),5.54-5.39(m,1H),5.35-5.19(m,2H),4.76-4.66(m,1H),4.63-4.53(m,1H),4.39-4.14(m,4H) MS m / z=615.4[M+H] + .

[0413] Example 2 [ka]

[0414] Step 1: Synthesis of intermediate 2-2

[0415] Compound 2-1 (11.0 g, 18.66 mmol) was added to anhydrous methanol (150 mL), followed by the addition of ammonium acetate (7.19 g, 93.29 mmol). The resulting reaction solution was heated to 60° C. and stirred for 48 hours. The reaction solution was concentrated, and the residue was dissolved in 300 mL of ethyl acetate. Then, 50 mL of water was added, and the mixture was stirred until it became clear. The aqueous layer was separated, and the organic phase was washed with water (30 mL*2) and saturated brine (30 mL). The organic phase was concentrated to obtain the crude product, which was purified by column chromatography (petroleum ether:ethyl acetate=30:1) to obtain compound 2-2. 1H NMR(400MHz,CDCl3)δ:7.16(d,J=8.4Hz,4H),6.84(d,J=8.4Hz,4H),6.62(d,J=8.4Hz,1H),4.99(br,J=10.5Hz,1H),4.63(d,J=1 MS m / z=589.1[M+H] + .

[0416] Step 2: Synthesis of intermediate 2-3

[0417] Compound 2-2 (7.56 g, 12.84 mmol) and N,N-diisopropylethylamine (3.32 g, 25.69 mmol, 4.47 mL) were dissolved in tetrahydrofuran (150 mL). The mixture was cooled to 0° C. under nitrogen, and then compound 2-2A (2.10 g, 15.41 mmol, 1.64 mL) was added dropwise. After the addition, the mixture was stirred at 15° C. for 15 hours. The reaction solution was concentrated to obtain the crude product, which was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to obtain compound 2-3. MS m / z=689.1 [M+H] + .

[0418] Step 3: Synthesis of intermediate 2-4

[0419] Compound 2-3 (2.94 g, 4.27 mmol) was dissolved in tetrahydrofuran (30 mL), and then sodium methoxide (461.27 mg, 8.54 mmol) and anhydrous methanol (0.5 mL) were added. The resulting reaction solution was stirred under nitrogen at 50° C. for 4 hours. The reaction solution was cooled to room temperature. The reaction solution was adjusted to pH 4-5 with 0.5 M hydrochloric acid. The solvent was then removed by rotary evaporation. The residue was dissolved in 150 mL of ethyl acetate, and then washed with 30 mL of water and 30 mL of saturated brine. The organic phase was concentrated to obtain the crude product, which was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to obtain compound 2-4. MS m / z=657.2[M+H]+ .

[0420] Step 4: Synthesis of intermediate 2-5

[0421] Compound 2-4 (200 mg, 304.59 μmol) was dissolved in dimethyl sulfoxide (10 mL), and then lithium chloride (51.65 mg, 1.22 mmol, 24.95 μL) was added. The resulting reaction solution was heated to 120° C. and stirred for 4 hours. The mixture was diluted with 150 mL of ethyl acetate, and then washed with water (20 mL*3) and saturated brine (20 mL). The organic phase was concentrated to obtain the crude product, which was purified by column chromatography (methanol:dichloromethane=1:10) to obtain compound 2-5. MS m / z=599.2[M+H] + .

[0422] Step 5: Synthesis of intermediate 2-6

[0423] Compound 2-5 (400 mg, 668.24 μmol) and PhNTf2 (477.46 mg, 1.34 mmol) were added to N,N-dimethylformamide (1.5 mL). N,N-diisopropylethylamine (345.46 mg, 2.67 mmol, 465.58 μL) was added. The reaction solution was stirred at 15° C. for 3 hours. The reaction solution was concentrated to obtain a crude product. This was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to obtain compound 2-6. MS m / z=862.8[M+H] + .

[0424] Step 6: Synthesis of intermediate 2-7

[0425] Compound 2-6 (580 mg, 672.30 μmol) and compound 1-1A (142.72 mg, 672.30 μmol) were added to N,N-dimethylformamide (4 mL). N,N-diisopropylethylamine (173.78 mg, 1.34 mmol, 234.21 μL) was added. The resulting reaction solution was heated to 60° C. and stirred for 3 hours. The reaction solution was concentrated to obtain a crude product. The crude product was purified by column (petroleum ether: ethyl acetate = 10:1) to obtain compound 2-7. MS m / z = 925.4 [M + H] + .

[0426] Step 7: Synthesis of intermediates 2-8A and 2-8B

[0427] Compound 2-7 (106 mg, 114.60 μmol) and compound 1-2A (54.74 mg, 343.81 μmol) were added to dioxane (2 mL). Pd2(dba)3 (20.99 mg, 22.92 μmol), DavePhos (18.04 mg, 45.84 μmol) and LIHMDS (1M, 343.81 μL) were added. The atmosphere was replaced with nitrogen and the mixture was heated to 95 °C for 15 h. The reaction solution was concentrated. Water (1 mL) and ethyl acetate (30 mL) were added to the residue. The mixture was adjusted to pH 7-8 with 0.5 M hydrochloric acid. The organic phase was separated. The aqueous phase was extracted twice with 10 mL of ethyl acetate. The organic phases were combined and concentrated to obtain the crude product. The crude product was separated on a preparative plate (ethyl acetate:methanol = 20:1). The sample separated from the above separation plate was further purified and concentrated by high performance liquid chromatography (chromatography column: Welch Xtimate C18 100*40mm*3μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; (acetonitrile)%: 43%-73%, 8 min) to obtain compound 2-8A (MS m / z=934.9[M+H] + ) and compound 2-8B (MS m / z=934.9[M+H] + ) was obtained.

[0428] Step 8: Synthesis of Compound 2A Hydrochloride and Compound 2B Hydrochloride

[0429] Trifluoroacetic acid (0.3 mL) was added to compound 2-8A (43 mg, 46.04 μmol) and the mixture was stirred under nitrogen at 20° C. for 2 hours. The reaction solution was concentrated to obtain the crude product. The crude product was dissolved in acetonitrile and separated by high performance liquid chromatography (chromatography column: Xtimate C18 150*40 mm*5 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; (acetonitrile)%: 5%-35%, 10 min) to obtain compound 2A hydrochloride. MS m / z=594.3[M+H] + .

[0430] Trifluoroacetic acid (0.3 mL) was added to compound 2-8B (52 mg, 55.67 μmol), and the resulting reaction solution was stirred under nitrogen at 20° C. for 2 hours. The reaction solution was concentrated to obtain a crude product. The crude product was purified by high performance liquid chromatography (chromatography column: Xtimate C18 150*40 mm*5 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; (acetonitrile)%: 5%-35%, 10 min) to obtain compound 2B hydrochloride. LCMS m / z=594.4[M+H] + .

[0431] Example 3 [ka]

[0432] Step 1: Synthesis of intermediate 3-2

[0433] Compound 3-1 (50 g, 139.46 mmol) was added to anhydrous dichloromethane (500 mL). N,N-diisopropylethylenediamine (54.07 g, 418.39 mmol, 72.87 mL) was added. The mixture was cooled to 0°C, and chloromethyl methyl ether (15.59 g, 193.64 mmol, 14.71 mL) was slowly added dropwise. The resulting mixture was slowly warmed to 18°C ​​for 1 h. The reaction solution was added to 500 mL of ice water. The mixture was extracted with DCM (100 mL*2). The organic phases were combined, then washed with 500 mL of saturated sodium carbonate, 500 mL of saturated ammonium chloride, and 500 mL of half-saturated brine, respectively, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=3:1) to give compound 3-2. MS m / z=403.2[M+H] + .

[0434] Step 2: Synthesis of intermediate 3-3

[0435] Compound 3-2 (36 g, 89.42 mmol) and N,N-diisopropylethylenediamine (34.67 g, 268.27 mmol, 46.73 mL) were added to anhydrous dichloromethane (360 mL). The mixture was cooled to -40°C, and trifluoromethanesulfonic anhydride (37.85 g, 134.14 mmol, 22.13 mL) was added dropwise. The mixture was reacted for 1 hour. The reaction solution was added to 300 mL of ice water. The mixture was separated and extracted. The organic phase was washed successively with 200 mL of saturated sodium bicarbonate solution, 200 mL of saturated ammonium chloride, and 200 mL of brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to give compound 3-3. 1 H NMR(400MHz,CDCl3)δ=7.71(dd,J=5.2,9.2Hz,1H),7.43(d,J=2.4Hz,1H),7.36(d, J=2.0Hz,1H),7.33(t,J=8.8Hz,1H),5.28(s,2H),3.53(s,3H),1.28-1.18(m,21H).

[0436] Step 3: Synthesis of intermediate 3-4

[0437] Compound 3-3 (34 g, 63.59 mmol) was dissolved in N,N-dimethylformamide (340 mL). Vinyl tributyltin (42.03 g, 132.55 mmol, 38.56 mL) and lithium chloride (10.78 g, 254.38 mmol, 5.21 mL) were added. The atmosphere was replaced with nitrogen three times. Triphenylphosphine palladium dichloride (4.46 g, 6.36 mmol) was added under nitrogen. The mixture was reacted at 30° C. for 20 hours. 300 mL of 20% aqueous KF solution and 300 mL of methyl tert-butyl ether were added to the reaction solution. The mixture was stirred for 20 minutes and then filtered through a Celite® pad. The filter cake was rinsed with methyl tert-butyl ether (50 mL*4). The aqueous phase was removed and the organic phase was washed with 500 mL of saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure. The crude product was purified by column (petroleum ether:ethyl acetate=5:1) to give compound 3-4. MS m / z=413.3[M+H] + .

[0438] Step 4: Synthesis of intermediate 3-5

[0439] Compound 3-4 (20 g, 48.47 mmol) was added to anhydrous tetrahydrofuran (200 mL) and water (50 mL). The mixture was cooled to 0° C. Sodium periodate (31.10 g, 145.42 mmol, 8.06 mL) and osmium tetroxide (1.5 g, 5.90 mmol, 306.12 μL) were added. The resulting mixture was slowly warmed to 18° C. The reaction mixture was allowed to react for 1 hour. The reaction mixture was added to 300 mL of 10% sodium thiosulfate solution. The mixture was extracted with ethyl acetate (100 mL*2), washed with 500 mL of saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The crude product was purified by column (petroleum ether:ethyl acetate=5:1) to give compound 3-5. MS m / z=415.3[M+H] + .

[0440] Step 5: Synthesis of intermediate 3-7

[0441] To a dichloromethane solution of compound 3-6 (1 g, 4.05 mmol) and 1-1A (1.03 g, 4.86 mmol) was added N,N-diisopropylethylamine (1.05 g, 8.11 mmol). The resulting mixture was stirred at room temperature of 25° C. for 1 hour. Water (20 mL) and dichloromethane (50 mL) were added to the reaction solution. The mixture was stirred for 5 minutes. The aqueous phase was removed and the organic phase was concentrated under reduced pressure. The residue was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to give compound 3-7. MS m / z=423.1[M+H] + .

[0442] Step 6: Synthesis of intermediate 3-8

[0443] Lithium tetrahydridoaluminate (0.1 g, 2.84 mmol) was added in portions to a solution of compound 3-7 (0.6 g, 1.42 mmol) in tetrahydrofuran (5 mL) at 0° C. (in an ice-water bath) under nitrogen. The resulting mixture was allowed to warm to 25° C. and stirred for 2 h. Water (0.1 g), 15% aqueous sodium hydroxide solution (0.1 g), and water (0.3 g) were added dropwise to the reaction solution in sequence. The mixture was stirred for 30 min and then filtered. The filter cake was washed with tetrahydrofuran (10 mL). The filtrate was concentrated under reduced pressure to give compound 3-8. MS m / z=381.1 [M+H] + .

[0444] Step 7: Synthesis of intermediate 3-9

[0445] Lithium diisopropylamide (1.51 mL, 3.02 mmol, 2 M) was added dropwise to a solution of compound 3-8 (0.5 g, 1.33 mmol) in tetrahydrofuran (10 mL) at -65°C (in a dry ice-ethyl acetate bath) under nitrogen. After stirring for 30 minutes, a solution of compound 3-5 (0.5 g, 1.51 mmol) in tetrahydrofuran (5 mL) was added dropwise. The resulting mixture was allowed to warm to 25°C naturally. 0.1 M diluted aqueous hydrochloric acid (15 mL) and ethyl acetate (20 mL) were added to the reaction solution. The mixture was stirred for 10 minutes. The aqueous phase was removed, the organic phase was concentrated under reduced pressure, and the residue was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to give compound 3-9. MS m / z=795.4[M+H] + .

[0446] Step 8: Synthesis of intermediate 3-10

[0447] To a solution of compound 3-9 (0.51 g, 641.44 μmol) in tetrahydrofuran (10 mL) was added n-butyllithium (2.5 M, 564.47 μL) under nitrogen at −65° C. (dry ice-ethyl acetate bath). After stirring for 30 min, a solution of p-toluenesulfonyl chloride (183.43 mg, 962.16 μmol) in tetrahydrofuran (3 mL) was added dropwise. The resulting mixture was allowed to warm to 25° C. and stirred for 2 h. The reaction was quenched by adding 50 mL of saturated aqueous ammonium chloride solution. The mixture was extracted with 50 mL of ethyl acetate. The aqueous phase was removed. The organic phase was concentrated under reduced pressure. The residue was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to give compound 3-10. MS m / z=777.4[M+H] + .

[0448] Step 9: Synthesis of intermediate 3-11

[0449] Metachloroperbenzoic acid (14.11 mg, 69.49 μmol, content 85%) was added to a solution of compound 3-10 (45 mg, 57.91 μmol) in dichloromethane (2 mL). The resulting mixture was stirred at room temperature of 25° C. for 2 hours. 2 mL of saturated aqueous sodium bicarbonate solution, 2 mL of saturated aqueous sodium sulfite solution, and 5 mL of dichloromethane were added to the reaction solution, and the mixture was stirred for 5 minutes. The aqueous phase was removed, and the organic phase was concentrated under reduced pressure. The residue was purified by preparative TLC (petroleum ether:ethyl acetate=5:1) to obtain compound 3-11. MS m / z=793.4[M+H] + .

[0450] Step 10: Synthesis of intermediate 3-12

[0451] To a solution of compound 1-2A (32.12 g, 201.76 μmol) in tetrahydrofuran (2 mL) was added sodium tert-butoxide (19.39 mg, 201.76 μmol) under nitrogen at 0° C. (ice-water bath). After stirring for 30 min, compound 3-11 (40 mg, 50.44 μmol) was added. The resulting mixture was allowed to warm to 25° C. and stirred for 2 h. The reaction solution was adjusted to pH 6 by adding 0.5 M dilute hydrochloric acid. 5 mL of ethyl acetate and 2 mL of saturated brine were added. The mixture was stirred for 5 min. The aqueous phase was removed and the organic phase was concentrated under reduced pressure to give compound 3-12. MS m / z=888.5[M+H] + .

[0452] Step 11: Synthesis of intermediate 3-13 hydrochloride

[0453] To a solution of compound 3-12 (45 mg, 50.67 μmol) in dichloromethane (1 mL), hydrochloric acid-ethyl acetate (4 M, 126.67 μL) was added. The resulting mixture was stirred at room temperature for 2 hours, and the reaction solution was directly concentrated under reduced pressure to give compound 3-13 hydrochloride. MS m / z=744.4[M+H] + .

[0454] Step 12: Synthesis of intermediate 3-14

[0455] To a solution of compound 3-13 hydrochloride (0.5 g, 612.06 μmol) in MeOH (10 mL) was added NaHCO3 (2M, 1.53 mL) and Boc2O (267.16 mg, 1.22 mmol, 281.22 μL). The mixture was stirred at room temperature for 2 h. The reaction solution was concentrated under reduced pressure to remove methanol. To the residue was added 10 mL of ethyl acetate and 5 mL of saturated brine, and the mixture was stirred for 5 min. The aqueous phase was removed and the organic phase was concentrated under reduced pressure to give compound 3-14. MS m / z=844.4 [M+H] + .

[0456] Step 13: Synthesis of intermediate 3-15

[0457] To a solution of compound 3-14 (1.2 g, 1.42 mmol) in DCM (5 mL) was added Et3N (287.70 mg, 2.84 mmol, 395.74 μL) and Tf2O (601.64 mg, 2.13 mmol, 351.83 μL). The mixture was stirred at room temperature for 2 h. 10 mL of water and 10 mL of dichloromethane were added to the reaction solution and the mixture was stirred for 10 min. The aqueous phase was removed. The organic phase was concentrated under reduced pressure. The residue was purified by column chromatography (petroleum ether:ethyl acetate=1:1) to give compound 3-15. MS m / z=976.2[M+H] + .

[0458] Step 14: Synthesis of intermediate 3-16

[0459] Compound 3-15 (200 mg, 204.88 μmol), compound 3-15A (74.26 mg, 409.76 μmol, 68.76 μL), cesium carbonate (200.26 mg, 614.64 μmol) and Xantphos (23.71 mg, 40.98 μmol) were added to anhydrous toluene (5 mL). The atmosphere was replaced with nitrogen three times. Tris(dibenzylideneacetone)dipalladium (18.76 mg, 20.49 μmol) was added. The mixture was reacted at 100° C. for 3 hours under nitrogen. The reaction solution was cooled to room temperature and filtered through a Celite® pad and rinsed with 30 mL of ethyl acetate. The mother liquor was concentrated under reduced pressure to give compound 3-16.

[0460] Step 15: Synthesis of intermediate 3-17 hydrochloride

[0461] Compound 3-16 (180 mg, 178.69 μmol) was added to a 4 M hydrochloric acid / methanol solution (5 mL). The mixture was reacted at room temperature for 1 hour. The reaction solution was directly concentrated to obtain crude compound 3-17 hydrochloride. MS m / z=743.3 [M+H] + .

[0462] Step 16: Synthesis of compound 3 hydrochloride

[0463] Compound 3-17 hydrochloride (180.00 mg), cesium fluoride (73.60 mg, 484.51 μmol) and anhydrous potassium carbonate (267.86 mg, 1.94 mmol) were added to N,N-dimethylformamide (10 mL). The mixture was reacted at 60° C. for 16 hours. 10 mL of water was added to the reaction solution. The mixture was extracted with 10 mL*2 of ethyl acetate. The organic phases were combined, washed with saturated brine (10 mL*3), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The product was purified by high performance liquid chromatography (chromatography column: Phenomenex luna C18 80*40 mm*3 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; (acetonitrile)%: 1%-20%, 7 min) to obtain compound 3 hydrochloride. 1 H NMR(400MHz,DMSO-d6)δ=7.75-7.63(m,1H),7.42-7.24(m,2H),6.91-6.8 1(m,1H),6.57-6.41(m,1H),5.58-5.45(m,2H),5.36-5.14(m,1H),5.06-4 .90(m,1H),4.84-4.58(m,2H),3.97-3.78(m,3H),3.66-3.38(m,2H),3.10 -2.89(m,4H),2.85-2.64(m,1H),2.15-1.89(m,6H),1.87-1.56(m,9H).MS m / z=587.3[M+H] + .

[0464] Example 4 [ka]

[0465] Step 1: Synthesis of intermediate 1-13B

[0466] Compound 1-12 (4.16 g, 26.15 mmol) was dissolved in anhydrous tetrahydrofuran (150 mL) under nitrogen. The mixture was cooled to 20° C. Sodium tert-butoxide (2.85 g, 29.64 mmol) was added. The mixture was stirred for 0.5 h. A solution of compound 1-2A (15 g, 17.43 mmol) in THF (15 mL) was added dropwise to the reaction solution. The mixture was stirred at this temperature for 0.5 h. The reaction solution was poured into saturated aqueous ammonium chloride solution (200 mL). The mixture was extracted with ethyl acetate (100 mL×3). The organic phases were combined and concentrated to give the crude product. The crude product was purified by high performance liquid chromatography (chromatography column: Phenomenex luna C18 (250*70mm, 15μm); mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; acetonitrile%: 40%-70%, 20min) and separated again (chromatography column: Phenomenex luna C18 250mm*100mm*10μm; mobile phase: [water (0.025% trifluoroacetic acid)-acetonitrile]; acetonitrile%: 40%-70%, 20min) to obtain the product. This was purified by supercritical fluid chromatography (SFC) (chromatography column: DAICEL CHIRALPAK IE (250 mm * 30 mm, 10 μm); mobile phase: A: supercritical CO2, B: [acetonitrile / isopropanol = 1: 1 (0.1% ammonia water)]; B%: 65%-65%, 45 minutes) to obtain compounds 1-13A and 1-13B. SFC analysis: (chromatography column: DAICEL CHIRALPAK IE (50 mm * 4.6 mm, 3 μm); mobile phase: A: supercritical CO2, B: [acetonitrile / isopropanol = 1: 1 (0.1% diisopropylamine)]; B%: 50%-50%), compound 1-13A (retention time = 2.143); compound 1-13B (retention time = 4.935). MS m / z = 955.5 [M + H] + .

[0467] Step 2: Synthesis of intermediate 4-1

[0468] Compound 1-13B (120 mg, 125.60 μmol), anhydrous potassium phosphate (53.32 mg, 251.19 μmol) and potassium vinyltrifluoroborate (25.24 mg, 188.39 μmol) were dissolved in 1,4-dioxane (1 mL) and water (0.2 mL). The atmosphere was replaced with nitrogen three times. Chloro[(n-butyldi(1-adamantyl)phosphine)-2-(2-aminobiphenyl)]palladium(II) (8.40 mg, 12.56 μmol) was added. The mixture was reacted at 80° C. for 3 hours. The reaction solution was poured into an aqueous solution (5 mL). The mixture was extracted with ethyl acetate (10 mL×2), separated, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated to give compound 4-1. MS m / z=947.4[M+H] + .

[0469] Step 3: Synthesis of compound 4 hydrochloride

[0470] Compound 4-1 (100 mg, 105.59 μmol) was dissolved in anhydrous dichloromethane (2 mL). Trifluoroacetic acid (770.00 mg, 6.75 mmol) was added. The mixture was reacted at 20° C. for 3 hours. The reaction solution was concentrated to obtain a crude product. The product was purified by high performance liquid chromatography (chromatography column: Phenomenex Luna 80*30 mm*3 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; acetonitrile%: 1%-30%, 8 min) to obtain compound 4 hydrochloride. MS m / z=607.4[M+H] + .

[0471] Example 5 [ka]

[0472] Step 1: Synthesis of intermediate 5-1

[0473] Compound 1-13B (100 mg, 104.66 μmol), potassium phosphate (55.54 mg, 261.66 μmol) and cyclopropylboronic acid (89.90 mg, 1.05 mmol) were added to 1,4-dioxane (2.5 mL) and water (0.5 mL). The atmosphere was replaced with nitrogen three times. Chloro(2-dicyclohexylphosphine-2',6'-dimethoxy-1,1'-biphenyl)[2-(2'-amino-1,1'-biphenyl)]palladium(II) (7.54 mg, 10.47 μmol) was added. The mixture was reacted at 100° C. for 3 hours under nitrogen. The reaction solution was cooled to room temperature and concentrated under reduced pressure to obtain crude compound 5-1. MS m / z=961.5[M+H] + .

[0474] Step 2: Synthesis of compound 5 hydrochloride

[0475] Compound 5-1 (150 mg, 156.08 μmol) was added with trifluoroacetic acid (1.60 g, 14.05 mmol). The mixture was reacted in anhydrous dichloromethane (5 mL) at 0° C. for 16 hours. The reaction solution was poured into 10 mL of water. The organic phase was separated. The aqueous phase was extracted with ethyl acetate (5 mL*2). The organic phases were combined, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated. The residue was purified by high performance liquid chromatography (chromatography column: Phenomenex Luna 80*30 mm*3 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; acetonitrile%: 1%-30%, 8 min) to obtain compound 5 hydrochloride. MS m / z=621.3[M+H] + .

[0476] Example 6 [ka]

[0477] Step 1: Synthesis of intermediate 6-1

[0478] Compound 1-13B (120 mg, 125.60 μmol), bis(acetonitrile)palladium(II) chloride (3.26 mg, 12.56 μmol), cesium carbonate (122.76 mg, 376.79 μmol) and 2-dicyclohexylphosphino-2',4',6'-triisopropyldiphenyl (11.97 mg, 25.12 μmol) were dissolved in acetonitrile (1.5 mL). The atmosphere was replaced with nitrogen three times. The mixture was stirred at room temperature for 0.5 hours. Trimethylsilylacetylene (49.34 mg, 502.38 μmol) was added to the reaction solution. The mixture was reacted at 80° C. for 10 hours. The reaction solution was directly concentrated to obtain compound 6-1. MS m / z=1017.5[M+H] + .

[0479] Step 2: Synthesis of Compound 6

[0480] Compound 6-1 (100 mg, 98.31 μmol) was dissolved in anhydrous dichloromethane (4 mL). Trifluoroacetic acid (1.54 g, 13.51 mmol) was added. The mixture was reacted at 20° C. for 3 hours. The mixture was poured into saturated aqueous sodium bicarbonate solution (5 mL). The layers were separated. The aqueous phase was extracted with dichloromethane (5 mL×3), washed with saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to give the crude product. The crude product was purified by preparative HPLC (chromatography column: Waters Xbridge BEH C18 100*30 mm*10 μm; mobile phase: [water (10 mM NH4HCO3)-acetonitrile]; acetonitrile%: 30%-50%, 8 min) to give compound 6. MS m / z=605.4[M+H] + .

[0481] Example 7 [ka]

[0482] Step 1: Synthesis of intermediate 7-1

[0483] Compound 1-13B (200 mg, 209.33 μmol) was dissolved in anhydrous toluene (2.5 mL). The mixture was bubbled with nitrogen for 10 minutes. Dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (44.47 mg, 62.80 μmol) and 1-propynyltri-n-butyltin (275.56 mg, 837.30 μmol) were added under nitrogen. The mixture was reacted at 110° C. for 3 hours. The reaction solution was poured into 5 mL of water. The mixture was extracted with ethyl acetate (3 mL×2). The organic phases were combined, washed with saturated brine (3 mL×2), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to give compound 7-1. MS m / z=959.3 [M+H] + .

[0484] Step 2: Synthesis of compound 7 hydrochloride

[0485] Compound 7-1 (450 mg, 469.21 μmol) was dissolved in dichloromethane (2.5 mL). Trifluoroacetic acid (802.50 mg, 7.04 mmol) was added. The mixture was reacted at 25° C. for 4 hours. The reaction solution was directly concentrated and purified by HPLC (chromatography column: Phenomenex luna C18 250*50 mm*10 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; acetonitrile%: 5%-35%, 10 min) to obtain compound 7 hydrochloride. MS m / z=619.2[M+H] + . 1 H NMR (400MHz, CD3OD) δ 7.00-6.92(m,1H),5.74-5.50(m,1H),5.31-5.17(m,1H),5.03-4.92(m,2H),4.79-4.72(m,2H),4.31-4.16(m,2H),4.09-3.92(m,4H),3.9 0-3.83(m,1H),3.64-3.54(m,1H),3.54-3.36(m,3H),3.11-3.01(m,1H),2.84-2.61(m,2H),2.57-2.47(m,1H),2.44-2.33(m,1H),2.36(br d,J=8.5Hz,6H),2.06-2.02(m,3H).

[0486] Example 8 [ka] [ka]

[0487] Step 1: Synthesis of intermediate 8-1A

[0488] Compound 8-1 was purified by preparative supercritical fluid chromatography (SFC) (chromatographic column: ChiralPak IH, 250*50mm, 10μm; mobile phase: A: supercritical CO2, B: [0.1% ammonia-ethanol]; B%: 20%-20%, runtime: 3.7min, peak times: 1.266min and 1.521min). The sample with peak time 1.521min was collected to obtain compound 8-1A. SFC analytical method (chromatographic column: Chiralpak IH-3, 100 × 4.6 mm ID, 3 μm; mobile phase: A (supercritical CO2) and B (ethanol, containing 0.1% isopropylamine); gradient: B% = 10–50%, 4 min; flow rate: 3.4 mL / min; wavelength: 220 nm; pressure: 2000 psi) for compound 8-1A (Rt = 1.489 min, ee value 98.82%). 1 H NMR(400MHz,CDCl3)δ=4.99-4.86(m,2H),4.26-3.95(m,3H),3.59(m,1H),3.00-2.88(m,1H),2.87-2.12(m,4H),1.91(s,1H),1.20-1.08(m,3H).

[0489] Step 2: Synthesis of intermediate 8-2

[0490] Lithium tetrahydridoaluminate (1.55 g, 40.15 mmol) was dissolved in anhydrous tetrahydrofuran (30 mL). The mixture was cooled to 0° C., and a solution of compound 8-1A (2.8 g, 13.38 mmol) in anhydrous tetrahydrofuran (20 mL) was added under nitrogen. The mixture was reacted at 70° C. for 1 hour. 1.5 mL of water, 1.5 mL of 15% NaOH solution, and 4.5 mL of water were added to the reaction solution at 0° C. The mixture was stirred for 20 minutes. The reaction solution was filtered. The filter cake was washed with 10 mL of tetrahydrofuran, and the filtrate was concentrated to give compound 8-2. 1 H NMR(400MHz,CDCl3)δ=4.99-4.86(m,2H),4.26-3.95(m,3H),3.59(m,1H),3.00-2.88(m,1H),2.74-2.27(m,4H),1.91(s,1H),1.20-1.08(m,3H).

[0491] Step 3: Synthesis of intermediate 1-11B

[0492] Compound 1-11 was purified by supercritical fluid chromatography (chromatographic column: (S,S) Whelk-01 100×4.6 mm ID, 5.0 μm; mobile phase: A: supercritical CO2, B: [0.1% ethanolamine-isopropanol]; B%: 50%, flow rate: 2.5 mL / min, retention times 2.705 min and 3.357 min). The sample with retention time 3.357 min was collected to obtain compound 1-11B. Preparative SFC (chiral column: REGIS (s,s) WHELK-O1 (250 mm*50 mm, 10 μm); mobile phase: A: supercritical CO2, B: [75% isopropanol-25% acetonitrile]; B%: 50%-50%). Chiral SFC analysis (chromatographic column: (S,S)Whelk-O1 100×4.6 mm ID, 5.0 μm; mobile phase: A: supercritical CO2, B: [75% isopropanol / 25% acetonitrile / 0.05% diethylamine]; B%: 50%-50%, flow rate: 2.5 mL / min), compound 1-11A (retention time = 2.536 min, ee value 99%); compound 1-11B (retention time = 3.317 min, ee value 99%). MS m / z = 844.5 [M+H] + .

[0493] Step 4: Synthesis of intermediate 1-12B

[0494] Compound 1-11B (1 g, 1.18 mmol) was added to 20 mL of dichloromethane. Metachloroperbenzoic acid (240.44 mg, 1.18 mmol, 85% purity) was added. The mixture was reacted at room temperature for 1 hour. 5 mL of 5% sodium thiosulfate solution was added to the reaction mixture. The mixture was extracted with dichloromethane (10 mL x 2). The organic phases were combined. The organic phase was washed with saturated brine (5 mL x 2), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated and purified by column chromatography (petroleum ether:ethyl acetate = 10:1-1:1) to give compound 1-12B. MS m / z = 860.4 [M + H] + .

[0495] Step 5: Synthesis of intermediate 8-3

[0496] Compound 8-2 (103.29 mg, 674.14 μmol) was dissolved in anhydrous tetrahydrofuran (3 mL). Sodium tert-butoxide (64.78 mg, 674.14 μmol) was added. The mixture was cooled to -15°C for 15 minutes. Compound 1-12B (0.29 g, 337.07 μmol) was added. The mixture was reacted at -15°C to 25°C for another hour. 5 mL of saturated ammonium chloride was added to the reaction solution. The mixture was extracted with ethyl acetate (5 mL x 2), washed with saturated saline (5 mL x 2), dried over anhydrous sodium sulfate, and concentrated. The crude product was purified by column chromatography (petroleum ether:ethyl acetate = 0:1) to give compound 8-3. MS m / z = 949.2 [M + H] + .

[0497] Step 6: Synthesis of intermediate 8-4

[0498] Compound 8-3 (0.24 g, 252.77 μmol) was dissolved in anhydrous toluene (2.5 mL). The reaction was bubbled with nitrogen for 10 min. Dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium (53.69 mg, 75.83 μmol, 53.69 μL) and tributyl(1-propynyl)tin (332.76 mg, 1.01 mmol) were added under nitrogen. The mixture was reacted at 110° C. for 2 h. The reaction solution was poured into 5 mL of water. The mixture was extracted with ethyl acetate (3 mL×2). The organic phases were combined, washed with saturated brine (3 mL×2), dried over anhydrous sodium sulfate, and concentrated to give crude compound 8-4. MS m / z=953.4[M+H] + .

[0499] Step 7: Synthesis of compound 8 hydrochloride

[0500] Compound 8-4 (100 mg, 104.92 μmol) was dissolved in trifluoroacetic acid (3 mL). The mixture was reacted at 25° C. for 0.5 h. After completion of the reaction, the reaction solution was directly concentrated and purified by HPLC (chromatography column: Xtimate C18 150*40 mm*5 μm; mobile phase: [water (0.05% HCl)-acetonitrile]; acetonitrile%: 5%-35%, 10 min) to obtain compound 8 hydrochloride. MS m / z=613.3[M+H] + . 1H NMR(400MHz,CD3OD)δ ppm6.99-6.93(m,1H),5.36(br d,J=6.75Hz,2H),5.24(br dd. d,J=14.38Hz,2H),3.85-3.77(m,1H),3.56(br d,J=14.63Hz,1H),3.45-3.37(m,1H),3.30-3.23(m,1H),3.15-3.01(m,2H),2.85(br d,J=15.63Hz,1H),2.45(br dd,J=12.01,6.50Hz,1H),2.31-2.03(m,11H).

[0501] Example 9 [ka]

[0502] Step 1: Synthesis of intermediate 9-2

[0503] Compound 9-1 (1.2 g, 6.62 mmol) was weighed and added to THF (50 mL). The mixture was cooled to -20°C. LiAlH4 (1 M, 6.62 mL) was added. The mixture was reacted at -20°C for 0.5 h. The reaction was quenched with water. The reaction solution was directly concentrated. The residue was purified by column chromatography (dichloromethane:methanol=10:1) to give compound 9-2. MS m / z=154.1[M+H] + .

[0504] Step 2: Synthesis of intermediate 9-3

[0505] Compound 9-2 (73.43 g, 479.26 μmol) was dissolved in anhydrous tetrahydrofuran (2 mL). Sodium tert-butoxide (46.06 mg, 479.26 μmol) was added. The mixture was cooled to -15°C for 15 min. Compound 1-12B (200 mg, 228.22 μmol) was added. The mixture was reacted at -15°C to 25°C for another 1 h. 5 mL of saturated ammonium chloride was added to the reaction solution. The mixture was extracted with ethyl acetate (5 mL x 2), washed with saturated brine (5 mL x 2), dried over anhydrous sodium sulfate, and concentrated. The residue was purified by column chromatography (petroleum ether:ethyl acetate = 0:1) to give compound 9-3. MS m / z = 949.4 [M + H] + .

[0506] Step 3: Synthesis of compound 9 hydrochloride

[0507] Compound 9-3 (0.12 g, 126.39 μmol) was dissolved in anhydrous dichloromethane (1 mL). The mixture was cooled to 0° C. Trifluoroacetic acid (302.62 mg, 2.65 mmol, 196.51 μL) was added, and the mixture was reacted at 25° C. for 2 h. The reaction solution was directly concentrated and purified by HPLC (chromatography column: Phenomenex Luna 80*30 mm*3 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; acetonitrile%: 5%-40%, run time 8 min) to obtain compound 9 hydrochloride. MS m / z=609.1[M+H] + , 1 H NMR(400MHz,CD3OD)δ=6.90(s,1H),6.13-5.78(m,2H),5.31-5.21(m,1H),4.98-4.89(m,3H),4.28-4.14 (m,2H),4.07-3.68(m,6H),3.54-3.33(m,4H),3.11-2.99(m,1H),2.61-2.49(m,2H),2.37-1.94(m,8H).

[0508] Example 10 [ka]

[0509] Step 1: Synthesis of intermediate 10-2

[0510] Compound 10-1 (1.0 g, 5.22 mmol) was dissolved in DMF (15 mL). K2CO3 (3.61 g, 26.09 mmol) was added. After stirring for 30 min, allyl bromide (757.46 mg, 6.26 mmol) was added. The mixture was reacted at room temperature for 5 h. The reaction solution was diluted with 80 mL of ethyl acetate and then filtered through a Celite® pad. The filtrate was concentrated under reduced pressure to remove the solvent to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to obtain compound 10-2. 1 H NMR (400MHz, CDCl3): δ=6.07-5.92(m,1H),5.88-5.73(m,1H),5.27-5.18(m,1H),5.16-5.08(m,2H),5.04-4.96(m,1H) ),3.72-3.61(m,3H),3.30-3.18(m,1H),3.10-2.93(m,2H),2.75-2.63(m,1H),2.34-2.17(m,1H),1.91-1.72(m,3H).

[0511] Step 2: Synthesis of intermediate 10-3

[0512] Compound 10-2 (325 mg, 1.66 mmol) was dissolved in toluene (36 mL). Grubbs 2nd generation catalyst (70.65 mg, 83.22 μmol) was added. The mixture was heated to 120° C. and stirred under nitrogen for 20 h. The reaction solution was concentrated to give the crude product, which was purified by column chromatography (dichloromethane:methanol=20:1) to give compound 10-3. 1 H NMR (400MHz, CDCl3): δ=5.94-5.77(m,2H),4.12-4.00(m,1H),3.83-3.72(m,3H),3.49-3 .41(m,1H),3.39-3.30(m,1H),2.74-2.59(m,1H),2.43-2.27(m,1H),1.93-1.82(m,3H).

[0513] Step 3: Synthesis of intermediate 10-4

[0514] Compound 10-3 (130 mg, 777.49 μmol) was dissolved in THF (3 mL). The mixture was cooled to 0° C. under nitrogen, and then lithium tetrahydridoaluminate (1 M, 777.49 μL) was added dropwise. The reaction was stirred at 0° C. for 0.5 h. The reaction solution was quenched with 200 μL of water. 20 mL of ethyl acetate was added. The mixture was stirred for 2 min. The mixture was dried over 2 g of anhydrous sodium sulfate and filtered through Celite®. The filtrate was concentrated under reduced pressure to remove the solvent to give compound 10-4. 1 H NMR (400MHz, CDCl3): δ=5.74-5.63(m,1H),5.59-5.47(m,1H),3.92-3.70(m,1H ),3.44-3.23(m,3H),3.14-2.99(m,1H),2.62-2.47(m,1H),1.76-1.51(m,5H).

[0515] Step 4: Synthesis of intermediate 10-5

[0516] Compound 10-4 (43.68 mg, 313.82 μmol) was dissolved in anhydrous tetrahydrofuran (2 mL). Sodium tert-butoxide (26.14 mg, 271.98 μmol) was added. The mixture was cooled to -15 °C for 15 min. Compound 1-12B (180 mg, 209.22 μmol) was added. The mixture was reacted at -15 °C to 25 °C for 1 h. 5 mL of saturated ammonium chloride was added to the reaction solution. The mixture was extracted with ethyl acetate (5 mL x 2), washed with saturated brine (5 mL x 2), dried over anhydrous sodium sulfate, and concentrated. The crude product was purified by column chromatography (petroleum ether: ethyl acetate = 1:0-0:1) to give compound 10-5. MS m / z = 935.2 [M + H] + .

[0517] Step 5: Synthesis of intermediate 10-6

[0518] Compound 10-5 (130.00 mg, 138.97 μmol) was dissolved in anhydrous toluene (1.5 mL). The mixture was bubbled with nitrogen for 10 minutes. Dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium (29.52 mg, 41.69 μmol, 29.52 μL) and tributyl(1-propynyl)tin (182.95 mg, 555.89 μmol) were added under nitrogen. The mixture was reacted at 110° C. for 2 hours. The reaction solution was poured into 5 mL of water. The mixture was extracted with ethyl acetate (3 mL×2). The organic phases were combined, washed with saturated brine (3 mL×2), dried over anhydrous sodium sulfate, and concentrated. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=0:1), then further purified by high performance liquid chromatography (chromatography column: Phenomenex Luna 80*30mm*3μm; mobile phase A: water (0.05% hydrochloric acid); mobile phase B: acetonitrile; run gradient: acetonitrile%: 35%-70%; run time: 8 min) to give compound 10-6. MS m / z=939.3[M+H] + .

[0519] Step 6: Synthesis of compound 10 hydrochloride

[0520] Compound 10-6 (30 mg, 31.95 μmol) was dissolved in anhydrous dichloromethane (0.5 mL). Trifluoroacetic acid (1.16 g, 10.16 mmol, 752.18 μL) was added at 0 °C, and the mixture was reacted at 0-25 °C for 3 h. The reaction solution was directly concentrated. The residue was purified by HPLC (chromatography column: Phenomenex Luna 80*30 mm*3 μm; mobile phase A: water (0.05% hydrochloric acid); mobile phase B: acetonitrile; run gradient: acetonitrile%: 5%-35%, runtime 8 min) and lyophilized to obtain compound 10 hydrochloride. MS m / z=599.1 [M+H] + .

[0521] Example 11 [ka] [ka]

[0522] Step 1: Synthesis of intermediate 11-2

[0523] Compound 11-1 (20 g, 56.53 mmol) was dissolved in hydrochloric acid / ethyl acetate (4 M, 120 mL). The mixture was reacted at 25° C. for 2 h. The reaction solution was directly concentrated to give crude 11-2 hydrochloride salt. The crude product was used directly in the next step.

[0524] Step 2: Synthesis of intermediate 11-3

[0525] Crude 11-2 hydrochloride (20 g) was dissolved in DMF (65 mL). Potassium carbonate (14.2 g, 102 mmol) was added. The mixture was reacted at 25° C. for 12 hours. The reaction solution was diluted with 500 mL of ethyl acetate, washed with water (300 mL×2) and saturated brine (300 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=2:1) ​​to obtain compound 11-3.

[0526] Step 3: Synthesis of intermediate 11-4

[0527] Compound 11-3 (7 g, 32.23 mmol) was dissolved in 2-methyltetrahydrofuran (75 mL). The atmosphere was replaced with nitrogen three times. A solution of red aluminum in toluene (37.2 g, 129 mmol, 35.8 mL, 70% purity) was added slowly at 10° C. under nitrogen. The mixture was reacted at 25° C. for 12 hours. The reaction solution was quenched by adding dropwise to 26.0% aqueous sodium tartrate solution. The mixture was extracted with 2-methyltetrahydrofuran (200 mL). The aqueous phase was extracted with 2-methyltetrahydrofuran (50 mL×3). The combined organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to give compound 11-4.

[0528] Step 4: Synthesis of intermediate 11-5 formate salt

[0529] Compound 11-4 (2.3 g, 13.4 mmol) was dissolved in DCM (30 mL). Imidazole (3.66 g, 53.7 mmol), DMAP (164 mg, 1.34 mmol) and TBDPSCl (7.38 g, 26.9 mmol, 6.90 mL) were added. The mixture was reacted at 45° C. for 12 h. Water (50 mL) was added to the reaction solution. The organic phase was separated and the aqueous phase was extracted with dichloromethane (50 mL). The organic phases were combined, washed with 50 mL of saturated brine, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated. Methyl tert-butyl ether (10 mL), n-heptane (21 mL) and HCl (2M, 21 mL) were added. The aqueous phase was separated, washed with a mixed solvent of methyl tert-butyl ether:n-heptane=1:2 (20mL*3), and adjusted to pH 7 with aqueous sodium carbonate solution. The mixture was extracted with 270mL ethyl acetate and 40mL ethyl acetate successively. The organic phases were combined, washed with 20mL saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to give R f =0.6 (first point) crude intermediate 11-5 was obtained. This was further purified by high performance liquid chromatography (chromatography column: Phenomenex Luna C18 250*80mm*10μm; mobile phase: [water (0.225% formic acid)-acetonitrile]; acetonitrile%: 32%-62%, 12 min) to obtain compound 11-5 formate. MS m / z=410.3[M+H] + . 1 H NMR(400MHz,CDCl3)δ ppm7.62-7.53(m,4H),7.37-7.27(m,6H),6.47-6.17(m,1H),3.66(br d,J=14.92Hz,1H),3.38-3.30(m,2H),3.25(br d,J=14.79Hz,1H),3.03-2.94(m,1H),2.56-2.43(m,2H),2.12(br d,J=15.41Hz,1H),1.92(ddd,J=12.20,7.92,3.97Hz,1H),0.99(s,9H)1.82-1.51(m,3H). 19F NMR(377MHz,CDCl3)δ ppm-131.66.

[0530] Step 5: Synthesis of intermediate 11-5A

[0531] Compound 11-5 (3.3 g, 7.57 mmol) was separated by preparative chiral SFC (chromatographic column: DAICEL CHIRALPAK IC (250 mm*30 mm, 10 μm); mobile phase: A: supercritical CO2, B: [0.1% NH3·H2O in methanol]; B%: 25%-25%, 4.5 min, peak times: 2.117 min and 2.980 min). The sample with peak time 2.117 min was collected to give compound 11-5A. SFC analysis method for compound 11-5A (chromatographic column: Chiralpak IC-3 50×4.6 mm ID, 3 μm; mobile phase: A (supercritical CO2) and B (MeOH, containing 0.05% diethylamine); gradient: B%=5-10%, flow rate: 3 mL / min), Rt=2.014 min, ee value 98.5%. MS m / z=410.3[M+H] + .

[0532] Step 6: Synthesis of intermediate 11-6 hydrochloride

[0533] Compound 11-5A (1.2 g, 2.93 mmol) was dissolved in 24 mL of 1,4-dioxane. Concentrated hydrochloric acid (12 M, 7.20 mL) was added. The mixture was reacted at 95° C. for 12 hours. The reaction solution was cooled and diluted with 10 mL of water. The mixture was washed with 10 mL of ethyl acetate. The aqueous phase was lyophilized to give compound 11-6 hydrochloride. 1H NMR(400MHz,D2O)δ ppm6.90-6.53(m,1H),4.22(br d,J=15.04Hz,1H),3.97(br d,J=15.04Hz,1H),3.79-3.69(m,1H),3.67-3.55(m,2H),3.24-3.14(m,1H),2.76-2.67(m,1H),2.64-2.54(m,1H),2.19-1.94(m,4H). 19 F NMR(376MHz,CDCl3)δ ppm-126.73.

[0534] Step 7: Synthesis of intermediate 11-7

[0535] Compound 1-12B (300 mg, 349 μmol) was dissolved in anhydrous toluene (10.0 mL). Sodium tert-butoxide (134 mg, 1.39 mmol, 4 equiv.), 4A molecular sieves (500 mg) and compound 11-6 hydrochloride (109 mg, 523 μmol) were added. The mixture was reacted at 110° C. for 12 hours. The reaction solution was quenched by adding 30 mL of saturated aqueous ammonium chloride solution. The mixture was extracted with ethyl acetate (30 mL). The organic phase was washed with saturated brine (20 mL), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=1:1) to give compound 11-7. 1 H NMR(400MHz,CDCl3)δ ppm7.14(d,J=8.56Hz,4H),6.87(d,J=8.68Hz,5H),6.64-6.36(m,1H),5.28-5.18(m,1H),4.79-4.68(m,2H) ),4.44-4.24(m,6H),4.10-3.97(m,2H),3.94-3.74(m,8H),3.51-2.94(m,7H),2.77-2.55(m,2H),2.32(br d,J=15.16Hz,1H),2.22-2.11(m,1H),1.95-1.58(m,7H),1.51(s,9H). 19 F NMR(376MHz,CDCl3)δ ppm-119.86,-50.65.

[0536] Step 8: Synthesis of intermediate 11-8

[0537] Compound 11-7 (222 mg, 229 μmol) was dissolved in anhydrous toluene (10.0 mL). 1-Propynyltri-n-butyltin (755 mg, 2.29 mmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (162 mg, 229 μmol, 162 μL) were added. The atmosphere was replaced with nitrogen three times. The mixture was reacted at 110° C. for 12 hours under nitrogen. The reaction solution was cooled and concentrated to obtain the crude product. This was purified by column chromatography (petroleum ether:ethyl acetate=1:1) to obtain compound 11-8. MS m / z=971.3[M+H] + .

[0538] Step 9: Synthesis of Compound 11 Formate

[0539] Compound 11-8 (149 mg, 129 μmol, 84% purity) was dissolved in dichloromethane (10 mL). Trifluoroacetic acid (770 mg, 6.75 mmol, 0.5 mL) was added. The mixture was reacted at 25° C. for 12 hours. The reaction solution was diluted with 10 mL of water and 20 mL of ethyl acetate. The layers were separated. The organic phase was washed with 1N dilute hydrochloric acid (5 mL×2). All aqueous phases were combined and adjusted to pH 8 with saturated aqueous NaHCO3. The solution was concentrated. 20 mL of acetonitrile was added to the obtained solid, and the mixture was stirred and filtered. The filtrate was concentrated to obtain the crude product. The crude product was purified by high performance liquid chromatography (chromatography column: Phenomenex luna C18 150*25mm*10μm; mobile phase: [water (0.225% formic acid)-acetonitrile]; acetonitrile%: 9%-39%, 10 min) and lyophilized to obtain compound 11 formate. MS m / z=631.3[M+H] + . 1H NMR(400MHz,CD3OD)δ ppm6.93(d,J=8.44Hz,1H),6.90-6.65(m,1H),5.19(br dd,J=11.13,3.79Hz,1H),4.77-4.69(m,2H),4.41-4.31(m,2H),4.26(br d,J=13.57Hz,1H),4.19-4.04(m,3H),3.86-3.73(m,2H),3.68-3.61(m,1H),3 .52-3.44(m,1H),3.37(s,3H),3.32-3.24(m,2H),3.06-2.81(m,3H),2.62(br d,J=16.14Hz,1H),2.31-2.05(m,6H),2.02-1.91(m,2H).

[0540] Example 12 [ka]

[0541] Step 1: Synthesis of intermediate 12-2

[0542] Compound 12-1 (20.0 g, 73.7 mmol) and bis-(4-methoxybenzyl)-amine (37.9 g, 147 mmol) were added to N-methylpyrrolidone (600 mL). The mixture was heated in an oil bath at 140° C. for 12 hours. The reaction solution was diluted with 250 mL of water and washed with ethyl acetate (200 mL×3) and 200 mL of saturated brine. The organic phase was dried and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column (petroleum ether:ethyl acetate=20:1) to give compound 12-2.

[0543] Step 2: Synthesis of intermediate 12-3

[0544] Compound 12-2 (22.0 g, 49.1 mmol) was dissolved in anhydrous tetrahydrofuran (220 mL). The mixture was cooled to -78 °C under nitrogen, and then n-butyllithium (2.5 M, 21.6 mL) was added dropwise. After the addition was complete, the reaction was stirred for 1 h, and then N,N-dimethylformamide (71.8 g, 982 mmol, 75.6 mL) was added dropwise. The mixture was stirred and reacted for 1 h. The reaction was quenched by adding 100 mL of water. The mixture was extracted with ethyl acetate (100 mL x 3). The organic phases were combined and concentrated under reduced pressure to give the crude product. The crude product was purified by column (petroleum ether:ethyl acetate = 20:1) to give compound 12-3. MS m / z = 397.1 [M + H] + .

[0545] Step 3: Synthesis of intermediate 12-4

[0546] Compound 12-3 (15.0 g, 37.8 mmol) was dissolved in N,N-dimethylformamide (170 mL). N-bromosuccinimide (7.06 g, 39.6 mmol) was added. The mixture was reacted at room temperature of 25 °C for 1 h under nitrogen. The reaction was quenched by adding 300 mL of water. The mixture was extracted with ethyl acetate (200 mL x 3). The organic phases were combined and concentrated under reduced pressure to give the crude product. The crude product was purified by column (petroleum ether:ethyl acetate = 10:1) to give compound 12-4. 1 H NMR(400MHz,CDCl3)δ ppm10.10(s,1H),7.16(d,J=8.63Hz,4H),6.89-6.85(m,4H),6.76(s,1H),4.73(s,4H),3.81(s,6H).

[0547] Step 4: Synthesis of intermediate 12-5

[0548] Compound 12-4 (14.2 g, 29.8 mmol) and methyl fluorosulfonyldifluoroacetate (28.6 g, 149 mmol, 18.9 mL) were added to N,N-dimethylformamide (160 mL). Copper iodide (5.68 g, 29.8 mmol) was added. The atmosphere was replaced with nitrogen and the mixture was stirred at 100 °C for 2.5 h. The reaction was quenched by adding 100 mL of water. The mixture was then extracted with ethyl acetate (300 mL x 3). The organic phases were combined and concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate = 20:1) to give compound 12-5. MS m / z = 465.0 [M + H] + .

[0549] Step 5: Synthesis of intermediate 12-6

[0550] Compound tetramethylpiperidine (1.45g, 10.2mmol) was dissolved in anhydrous tetrahydrofuran (100mL). The mixture was cooled to -40°C under nitrogen, and then n-butyllithium (2.5M, 32.8mL) was added dropwise. After the addition, the mixture was stirred for 0.5 hours. Compound 3-8 (7.80g, 20.5mmol) was dissolved in anhydrous tetrahydrofuran (50mL). The mixture was added dropwise to a reaction flask at -60°C. After the addition, the mixture was stirred for 0.3 hours. Compound 12-5 (10.4g, 22.5mmol) was added to the reaction at -60°C. After the addition, the mixture was stirred for 0.5 hours. The reaction was quenched by adding 300mL of water. The mixture was then extracted with ethyl acetate (400mL x 3). The organic phases were combined and concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to give compound 12-6. MS m / z=845.2[M+H] + .

[0551] Step 6: Synthesis of intermediate 12-7

[0552] Compound 12-6 (6.1 g, 7.22 mmol) was dissolved in anhydrous toluene (60 mL). Cyanomethylenetri-n-butylphosphine (5.22 g, 21.6 mmol) was added under nitrogen. After the addition, the reaction was stirred at 100° C. for 12 h. The reaction solution was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to give compound 12-7. MS m / z=827.2 [M+H] + . 1 H NMR(400MHz,CDCl3)δ ppm7.09(br d,J=8.50Hz,4H),6.84(br d,J=8.50Hz,4H),6.53(s,1H),5.20(br dd,J=10.01,3.00Hz,1H),4.81-4.54(m,7H),4.37-4.25(m,2H),3.80(s,7H), 3.67-3.55(m,1H),3.50-3.37(m,2H),3.14-2.92(m,2H),2.49(s,3H),1.94(br s,3H),1.50(s,9H).

[0553] Step 7: Synthesis of intermediate 12-8

[0554] Compound 12-7 (600 mg, 725 μmol) was dissolved in anhydrous dichloromethane (6 mL). Metachloroperbenzoic acid (176 mg, 870 μmol, 85% purity) was added under nitrogen. After the addition, the mixture was stirred at 25° C. for 1 h. The reaction was quenched by adding water (10 mL). The mixture was extracted with ethyl acetate (10 mL×3). The organic phases were combined and concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=2:1) ​​to give compound 12-8.

[0555] Step 8: Synthesis of intermediates 12-9A and 12-9B

[0556] Compound 12-8 (600 mg, 698 μmol) was dissolved in anhydrous toluene (5 mL). Sodium tert-butoxide (201 mg, 2.09 mmol) and compound 8-2 (128 mg, 837 μmol) were added under nitrogen. After the addition, the mixture was stirred at 80° C. for 12 h. The reaction was quenched by adding water (10 mL). The mixture was then extracted with ethyl acetate (10 mL×3). The organic phases were combined and concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=2:1) ​​to give compound 12-9. Compound 12-9 was subjected to preparative SFC chiral separation (chiral column: DAICEL CHIRALCEL OD (250 mm * 30 mm, 10 μm); mobile phase: [supercritical CO2-methanol (0.1% ammonia)]; methanol (0.1% ammonia) %: 40%-40%) to obtain compounds 12-9A and 12-9B. SFC analysis: (chiral column: DAICEL CHIRALCEL OD-3 (50 mm * 4.6 mm, 3 μm); mobile phase: [supercritical CO2-methanol (0.05% diethylamine)]; methanol %: 5%-40%), compound 12-9A (Rt = 2.091 min, ee value 99%; MS m / z = 932.4 [M + H] + ); Compound 12-9B (Rt=2.331 min, MS m / z=932.3[M+H] + ).

[0557] Step 9: Synthesis of Compound 12A Trifluoroacetate and Compound 12B Trifluoroacetate

[0558] Compound 12-9A (40.0 mg, 42.9 μmol) was dissolved in trifluoroacetic acid (1.54 g, 13.4 mmol, 1 mL). The mixture was reacted at 50° C. for 9 hours. The reaction solution was concentrated under reduced pressure, and the crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile%: 8%-38%, 8 min) to obtain compound 12A trifluoroacetate. MS m / z=592.2[M+H] + . 1H NMR(400MHz,CD3OD)δ ppm6.73(s,1H),5.34(br d,J=8.00Hz,2H),5.27(dd,J=10.13,3.25Hz,1H),4.98(br s,1H),4.74(d,J=13.76Hz,1H),4.69-4.59(m,2H),4.46(br d,J=14.26Hz,1H),4.37(br d,J=14.26Hz,1H),4.19(br d,J=13.13Hz,2H),3.99-3.92(m,2H),3.84-3.76(m,1H),3.73(br d,J=13.38Hz,1H),3.57(dd,J=18.26,10.01Hz,1H),3.42(br d,J=14.01Hz,1H),3.26(dt,J=11.48,7.08Hz,1H),3.07(br d,J=16.01Hz,1H),2.93(dd,J=18.32,3.19Hz,1H),2.82(br d,J=16.13Hz,1H),2.39(dd,J=11.88,6.50Hz,1H),2.31-2.10(m,6H),2.04-1.95(m,1H).

[0559] Compound 12-9B (40.0 mg, 42.9 μmol) was dissolved in trifluoroacetic acid (1.54 g, 13.4 mmol, 1 mL). The mixture was reacted at 50° C. for 9 hours. The reaction solution was concentrated under reduced pressure, and the crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile%: 8%-38%, 8 min) to obtain compound 12B trifluoroacetate. MS m / z=592.3[M+H] + . 1H NMR(400MHz,CD3OD)δ ppm6.71(s,1H),5.32(br d,J=8.50Hz,2H),5.25(dd,J=10.19,3.19Hz,1H),4.96(s,1H),4.73(d,J=13.76Hz,1H),4.63(s,2H),4.46(br d,J=14.13Hz,1H),4.36(br d,J=14.38Hz,1H),4.17(br d,J=13.01Hz,2H),4.00-3.89(m,2H),3.83-3.69(m,2H),3.55(dd,J=18.20,10.07Hz,1H),3.41(br d,J=14.13Hz,1H),3.24(dt,J=11.54,7.11Hz,1H),3.05(br d,J=16.01Hz,1H),2.91(dd,J=18.20,3.31Hz,1H),2.80(br d,J=16.26Hz,1H),2.43-2.33(m,1H),2.28-2.08(m,6H),2.03-1.93(m,1H).

[0560] Example 13 [ka]

[0561] Step 1: Synthesis of intermediates 13-1A and 13-1B

[0562] Compound 12-9A (250 mg, 268 μmol) was dissolved in anhydrous toluene (1 mL). 1-Propynyltri-n-butyltin (264 mg, 804 μmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (37.9 mg, 53.6 μmol) were added. The atmosphere was replaced with nitrogen three times. The mixture was reacted at 100° C. for 12 hours under nitrogen. The reaction solution was cooled and concentrated to obtain the crude product. The crude product was separated on a thin-layer separation plate (dichloromethane:methanol=15:1) to obtain compound 13-1A.

[0563] Refer to step 1 and use 12-9B as starting material to give compound 13-1B.

[0564] Step 2: Synthesis of Compound 13A Trifluoroacetate and Compound 13B Trifluoroacetate

[0565] Compound 13-1A (120 mg, 128 μmol) was dissolved in trifluoroacetic acid (1.54 g, 13.4 mmol, 1 mL). The mixture was reacted at 50° C. for 9 hours. The reaction solution was concentrated under reduced pressure. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile%: 10%-40%, 9 min) to obtain compound 13A trifluoroacetate. MS m / z=596.3[M+H] + . 1 H NMR(400MHz,CD3OD)δ ppm6.80(s,1H),5.43-5.26(m,3H),5.00(br d,J=13.88Hz,2H),4.81(d,J=13.63Hz,1H),4.60(d,J=2.50Hz,2H),4.45-4.30(m,2H),4.24-4. 13(m,2H),3.99-3.84(m,2H),3.83-3.64(m,2H),3.40-3.34(m,2H),3.28-3.21(m,1H),3.06(br d,J=16.26Hz,1H),2.95(dd,J=18.07,3.56Hz,1H),2.81(br d,J=16.01Hz,1H),2.38(br dd,J=11.63,6.38Hz,1H),2.29-2.12(m,8H),1.99(br t,J=9.44Hz,1H).

[0566] Compound 13-1B (120 mg, 128 μmol) was dissolved in trifluoroacetic acid (1.54 g, 13.4 mmol, 1 mL). The mixture was reacted at 50° C. for 9 hours. The reaction solution was concentrated under reduced pressure. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile%: 8%-38%, 8 min) to obtain compound 13B trifluoroacetate. MS m / z=596.2[M+H] + . 1 H NMR(400MHz,CD3OD)δ ppm6.80(s,1H),5.31(br d,J=8.88Hz,3H),4.99(br d,J=13.76Hz,2H),4.80(d,J=13.88Hz,1H),4.58(d,J=2.38Hz,2H),4.43-4.31(m,2H),4.16(br d,J=16.01Hz,2H),3.95-3.84(m,2H),3.79-3.67(m,2H),3.37(br d,J=13.63Hz,2H),3.28-3.22(m,1H),3.07-2.91(m,2H),2.80(br d,J=15.88Hz,1H),2.40-2.33(m,1H),2.23-2.10(m,8H),2.02-1.93(m,1H).

[0567] Example 14 [ka]

[0568] Step 1: Synthesis of intermediate 14-2

[0569] Compound 14-1 was dissolved in DMF (2.00 mL). Potassium tert-butoxide (299 mg, 2.67 mmol) was added to the reaction solution. The reaction solution was heated to 80° C. for 12 hours. Water (10 mL) was added to the reaction solution. The mixture was extracted with dichloromethane:methanol=5:1 (10 mL*3). The organic phases were combined, washed with saturated brine (20 mL), dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The crude product was purified by column (dichloromethane:methanol=5:1) to give compound 14-2. 1 H NMR(400MHz,CDCl3)δ ppm5.03(s,1H)3.89(ddd,J=15.04,5.07,2.14Hz,1H)3.38-3.44(m,1H)3.26-3.36(m,2H)3.13-3.22(m,1H)2.59-2.66(m,1H)1.61-1.90(m,5H); 19 F NMR(376MHz,CDCl3)δ ppm-128.29(br s,1F).

[0570] Step 2: Synthesis of intermediate 14-3

[0571] Compound 1-12B (120 mg, 139 μmol), compound 14-2 (43.9 mg, 279 μmol) and sodium tert-butoxide (40.2 mg, 418 μmol) were added to toluene (2.00 mL). The reaction solution was heated to 100° C. for 5 hours. The reaction solution was concentrated under reduced pressure. The crude product was purified by column (petroleum ether:ethyl acetate=1:1) to obtain compound 14-3. MS m / z=953.1 [M+H] + .

[0572] Step 3: Synthesis of intermediate 14-4

[0573] Compound 14-3 (75.0 mg, 78.7 μmol) and 1-propynyltri-n-butyltin (104 mg, 315 μmol) were added to toluene (2.00 mL). The atmosphere was replaced with nitrogen three times. Dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (16.7 mg, 23.6 μmol) was added to the reaction solution. The atmosphere was replaced with nitrogen three more times. The mixture was heated to 110° C. for 3 hours. The reaction solution was filtered using a Celite® pad, and the filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column (petroleum ether:ethyl acetate=1:1) to give compound 14-4. MS m / z=957.4[M+H] + .

[0574] Step 4: Synthesis of compound 14

[0575] Compound 14-4 (73.0 mg, 76.3 μmol) was dissolved in dichloromethane (1.00 mL). TFA (0.10 mL) was added to the reaction solution. The mixture was reacted at 25° C. for 3 hours. The reaction solution was adjusted to pH 7 with saturated sodium bicarbonate solution. The mixture was concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Waters Xbridge 150*25 mm*5 μm; mobile phase: [water (0.05% aqueous ammonia)-acetonitrile]; acetonitrile%: 53%-83%, 9 min) to obtain compound 14. MS m / z=617.4[M+H] + . 1H NMR(400MHz,CDCl3)δ ppm6.91-6.82(m,1H),5.49-5.38(m,1H),5.21-5.10(m,1H),4.75-4.69(m,2H),4.18-3.92(m,6H),3.65(br d,J=1.96Hz,2H),3.44(br s,1H),3.37-3.25(m,3H),3.10-3.04(m,1H),3.00-2.91(m,1H),2.67-2.58(m,1H),2.19-2 .08(m,1H),2.04(s,2H),2.03-1.97(m,1H),1.90-1.83(m,3H),1.78-1.72(m,3H),1.26(br s,1H),0.88-0.80(m,2H).

[0576] Example 15 [ka]

[0577] Step 1: Synthesis of intermediate 15-1

[0578] Compound 10-1 (3.00 g, 15.6 mmol) was dissolved in DMF (21 mL). Potassium carbonate (8.65 g, 62.6 mmol) and compound 3-bromo-2-methylpropene (2.54 g, 18.7 mmol) were added. The mixture was reacted at room temperature for 12 hours. 100 mL of water was poured into the reaction solution, and the mixture was extracted three times with ethyl acetate (10 mL). The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=20:1) to obtain compound 15-1.

[0579] Step 2: Synthesis of intermediate 15-2

[0580] Compound 15-1 (2.5 g, 11.9 mmol) was dissolved in anhydrous THF (20.0 mL). Lithium aluminum hydride (680 mg, 17.9 mmol) was added at 0° C. Then the mixture was reacted at room temperature for 1 hour. 1 mL of water, 1 mL of 15% aqueous NaOH solution, and 3 mL of water were added to the reaction solution. The mixture was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 15-2. MS m / z=182.1 [M+H] + .

[0581] Step 3: Synthesis of intermediate 15-3

[0582] Compound 15-2 (1.7 g, 9.38 mmol) was dissolved in anhydrous DCM (20.0 mL). Imidazole (2.55 g, 37.5 mmol) and 4-dimethylaminopyridine (114 mg, 937 μmol) were added, followed by tert-butyldiphenylsilyl chloride (5.16 g, 18.7 mmol) at 0° C. The mixture was reacted at room temperature for 12 h. 50 mL of water was added to the reaction solution, and the mixture was extracted three times with DCM (100 mL). The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=15:1) to give compound 15-3. MS m / z=420.4[M+H] + .

[0583] Step 4: Synthesis of intermediate 15-4

[0584] Compound 15-3 (2 g, 4.77 mmol) was dissolved in anhydrous toluene (200 mL). Grubbs catalyst (597 mg, 953 μmol) was added. The mixture was reacted at 110° C. for 12 hours. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by thin plate chromatography (dichloromethane:methanol=20:1) to obtain compound 15-4. MS m / z=392.2 [M+H] + .

[0585] Step 5: Synthesis of intermediate 15-5 hydrochloride

[0586] Compound 15-4 (200 mg, 510 μmol) was dissolved in anhydrous dioxane (1.0 mL). HCl (12 M, 212 μL) was added. The mixture was reacted at 95° C. for 12 hours. 10 mL of water was added to the reaction solution. After extraction, the aqueous phase was lyophilized to give a crude product that can be used directly in the next step. Compound 15-5 hydrochloride was obtained. MS m / z=154.1 [M+H] + .

[0587] Step 6: Synthesis of intermediate 15-6

[0588] Compound 1-12B (300 mg, 348 μmol) was dissolved in anhydrous toluene (2 mL). Sodium tert-butoxide (100 mg, 1.05 mmol) and compound 15-5 hydrochloride (64.1 mg) were added. The mixture was reacted at room temperature for 12 hours. 30 mL of saturated aqueous ammonium chloride solution was poured into the reaction solution, and the mixture was extracted with ethyl acetate (30 mL). The organic phase was washed with saturated brine (20 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (dichloromethane:methanol=10:1) to obtain compound 15-6. MS m / z=949.5[M+H] + .

[0589] Step 7: Synthesis of intermediate 15-7

[0590] Compound 15-6 (80 mg, 84.2 μmol) was dissolved in anhydrous toluene (2 mL). 1-Propynyltri-n-butyltin (83.1 mg, 252 μmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (11 mg, 16.8 μmol) were added. The atmosphere was replaced with nitrogen three times. The mixture was reacted under nitrogen at 110° C. for 2 hours. The reaction solution was cooled and concentrated under reduced pressure to obtain the crude product. The crude product was purified by thin plate chromatography (dichloromethane:methanol=10:1) to obtain compound 15-7. MS m / z=953.6[M+H] + .

[0591] Step 8: Synthesis of Compound 15 Trifluoroacetate

[0592] Compound 15-7 (40 mg, 41.9 μmol) was dissolved in dichloromethane (1.0 mL). Trifluoroacetic acid (4.79 mg, 41.9 μmol) was added. The mixture was reacted at 25° C. for 1 hour. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile%: 15%-45%, 9 min) and lyophilized to obtain compound 15 trifluoroacetate. MS m / z=613.5[M+H] + . 1 H NMR(CD3OD,400MHz)δ 6.92(d,J=8.5Hz,1H),5.48(br s,1H),5.20-5.10(m,1H),4.95-4.90(m,3H),4.80-4.70(m,1H),4.64(d,J=12.3Hz,1H),4.55-4.51(m,2H),4.33(br d,J=14.5Hz,1H),4.15(br dd,J=2.1,16.1Hz,2H),3.92(br d,J=15.4Hz,1H),3.82(br d,J=13.1Hz,1H),3.81-3.70(m,1H),3.71-3.61(m,1H),2.92(br dd,J=4.1,18.4Hz,1H),2.30-2.11(m,8H),2.01-1.91(m,4H),1.85(s,3H).

[0593] Example 16 [ka]

[0594] Step 1: Synthesis of intermediate 16-2

[0595] Compound 16-1 (500 mg, 2.56 mmol) was dissolved in anhydrous toluene (1 mL). Grubbs catalyst (217 mg, 256 μmol) and compound propylene / dichloromethane solution (0.5 M, 5.12 mL) were added. The mixture was reacted at 50° C. for 2 hours. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to obtain compound 16-2. MS m / z=210.0 [M+H] + .

[0596] Step 2: Synthesis of intermediate 16-3

[0597] Compound 16-2 (210 mg, 1.00 mmol) was dissolved in anhydrous THF (1.0 mL). Lithium aluminum hydride (76.1 mg, 2.01 mmol) was added at 0° C. The mixture was reacted at 70° C. for 12 hours. 0.7 mL of water, 0.7 mL of 15% aqueous NaOH, and 2 mL of water were added to the reaction solution. The mixture was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give the crude product, which was used directly in the next step. Compound 16-3 was obtained.

[0598] Step 3: Synthesis of intermediate 16-4

[0599] Compound 1-12B (300 mg, 348 μmol) was dissolved in anhydrous THF (1 mL). Sodium tert-butoxide (100 mg, 1.05 mmol) and compound 16-3 (58.3 mg, 348 μmol) were added. The mixture was reacted at room temperature for 12 hours. The reaction solution was quenched by adding 30 mL of saturated aqueous ammonium chloride solution. The mixture was extracted with ethyl acetate (30 mL). The organic phase was washed with saturated brine (20 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to obtain the crude product. The crude product was purified by column chromatography (dichloromethane:methanol=10:1) to obtain compound 16-4. MS m / z=963.5[M+H] + .

[0600] Step 4: Synthesis of intermediate 16-5

[0601] Compound 16-4 (90.0 mg, 93.4 μmol) was dissolved in anhydrous toluene (1 mL). 1-Propynyltri-n-butyltin (122 mg, 373 μmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (19.8 mg, 28.0 μmol) were added. The atmosphere was replaced with nitrogen three times. The mixture was reacted under nitrogen at 110° C. for 12 hours. The reaction solution was cooled and concentrated under reduced pressure to obtain the crude product. The crude product was purified by thin plate chromatography (dichloromethane:methanol=12:1) to obtain compound 16-5. MS m / z=967.5[M+H] + .

[0602] Step 5: Synthesis of Compound 16 Trifluoroacetate

[0603] Compound 16-5 (70.00 mg, 72.3 μmol) was dissolved in dichloromethane (1.0 mL). Trifluoroacetic acid (539 mg, 4.73 mmol) was added. The mixture was reacted at 25° C. for 12 hours. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile%: 18%-48%, 9 min) and lyophilized to obtain compound 16 trifluoroacetate. MS m / z=627.3[M+H] + .

[0604] Example 17 [ka]

[0605] Step 1: Synthesis of intermediate 17-2

[0606] Compound 1-12B (870 mg, 1.01 mmol) was dissolved in anhydrous toluene (10.0 mL). Sodium tert-butoxide (777 mg, 8.09 mmol) and compound 17-1 (404 mg, 3.03 mmol) were added. The mixture was reacted at 100° C. for 12 hours. 30.0 mL of saturated aqueous ammonium chloride solution was added to the reaction solution. The mixture was extracted with ethyl acetate (50.0 mL). The organic phase was washed with saturated brine (30.0 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=2:1) ​​to obtain compound 17-2. MS m / z=929.4[M+H] + .

[0607] Step 2: Synthesis of intermediate 17-3 formate salt

[0608] Compound 17-2 (445 mg, 479 μmol) was dissolved in anhydrous toluene (4.00 mL). 1-Propynyltri-n-butyltin (630 mg, 1.92 mmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (102 mg, 144 μmol) were added. The atmosphere was replaced with nitrogen three times. The mixture was reacted under nitrogen at 110° C. for 3 hours. The reaction solution was cooled and concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.225% formic acid)-acetonitrile]; acetonitrile%: 40%-70%, 10 min) and lyophilized to obtain compound 17-3 formate. MS m / z=933.5[M+H] + .

[0609] Step 3: Synthesis of compound 17

[0610] Compound 17-3 formate (210 mg) was dissolved in dichloromethane (2.00 mL). Trifluoroacetic acid (3.00 mL) was added. The mixture was reacted at 25° C. for 4 hours. The reaction solution was quenched by adding 6.00 mL of aqueous ammonia. The mixture was concentrated to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Waters Xbridge 150*25 mm*5 μm; mobile phase: [water (0.05% aqueous ammonia)-acetonitrile]; acetonitrile%: 28%-58%) and lyophilized to obtain compound 17. MS m / z=593.3[M+H] + .

[0611] Example 18 [ka]

[0612] Step 1: Synthesis of intermediate 18-2

[0613] Compound 18-1 (192.05 mg, 1.16 mmol) was dissolved in THF (10 mL). Sodium tert-butoxide (111.70 mg, 1.16 mmol) was added. The mixture was stirred at 0° C. for 1 h. Compound 1-12B (0.5 g, 581.16 μmol) was added. The mixture was reacted at 0° C. for 1 h. Water (10 mL) was added to the reaction solution. The mixture was extracted with ethyl acetate (20 mL*3). The organic phases were combined, washed with saturated brine (20 mL), and dried over anhydrous sodium sulfate. The filtrate was concentrated under reduced pressure. The residue was purified by column chromatography (dichloromethane:methanol=20:1) to give compound 18-2. MS m / z=961.7[M+H] + .

[0614] Step 2: Synthesis of intermediate 18-3

[0615] Compound 18-2 (0.526 g, 547.07 μmol) was dissolved in toluene (20 mL). Compound 1-propynyltri-n-butyltin (900.24 mg, 2.74 mmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (116.21 mg, 164.12 μmol) were added. The atmosphere was replaced with nitrogen five times. The mixture was reacted at 120° C. for 5 hours. Water (10 mL) was added to the reaction system. The mixture was filtered through a Celite® pad. The filter cake was washed with ethyl acetate (20 mL). The filtrate was extracted with ethyl acetate (20 mL*3). The organic phases were combined, washed with saturated brine (20 mL), and dried over anhydrous sodium sulfate. The filtrate was concentrated under reduced pressure. The residue was purified by column (dichloromethane:methanol=10:1) to obtain compound 18-3. MS m / z=965.7[M+H] + .

[0616] Step 3: Synthesis of compound 18 hydrochloride

[0617] Compound 18-3 (0.5 g, 518.09 μmol) was dissolved in DCM (10 mL). Trifluoroacetic acid (4.13 g, 36.25 mmol) was added. The mixture was reacted at 25° C. for 1 h. The reaction solution was concentrated under reduced pressure. The crude product was purified by HPLC (column: Xtimate C18 150*40 mm*5 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; (acetonitrile)%: 5%-35%, 10 min) to obtain compound 18 hydrochloride. MS m / z=625.3[M+H] + .

[0618] Example 19 [ka]

[0619] Step 1: Synthesis of intermediate 19-2

[0620] Compound 19-1 (199.00 mg, 1.16 mmol) was dissolved in THF (10 mL). Sodium tert-butoxide (111.70 mg, 1.16 mmol) was added. The reaction was stirred at 0° C. for 1 h. Compound 1-12B (0.5 g, 581.16 μmol) was added. The mixture was reacted at 0° C. for 1 h. Water (10 mL) was added to the reaction solution. The mixture was extracted with ethyl acetate (20 mL*3). The organic phases were combined, washed with saturated brine (20 mL), and dried over anhydrous sodium sulfate. The filtrate was concentrated under reduced pressure. The residue was purified by column chromatography (dichloromethane:methanol=20:1) to give compound 19-2. MS m / z=967.6[M+H] + .

[0621] Step 2: Synthesis of intermediate 19-3

[0622] Compound 19-2 (0.442 g, 456.87 μmol) was dissolved in toluene (10 mL). Compound 1-propynyltri-n-butyltin (601.44 mg, 1.83 mmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (97.05 mg, 137.06 μmol) were added. The atmosphere was replaced with nitrogen five times. The mixture was reacted at 120° C. for 5 hours. Water (10 mL) was added to the reaction system, and the mixture was filtered through a Celite® pad. The filter cake was washed with ethyl acetate (20 mL), and the filtrate was extracted with ethyl acetate (20 mL*3). The organic phases were combined, washed with saturated brine (20 mL), and dried over anhydrous sodium sulfate. The filtrate was concentrated under reduced pressure. The residue was purified by column chromatography (dichloromethane:methanol=10:1) to obtain compound 19-3. MS m / z=971.8[M+H] + .

[0623] Step 3: Synthesis of compound 19 hydrochloride

[0624] Compound 19-3 (0.442 g, 455.17 μmol) was dissolved in DCM (5 mL). Trifluoroacetic acid (15.58 g, 136.67 mmol) was added. The mixture was reacted at 25° C. for 1 h. The reaction solution was directly concentrated under reduced pressure, and the crude product was purified by HPLC (column: Xtimate C18 150*40 mm*5 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; (acetonitrile)%: 5%-35%, 10 min) to obtain compound 19 hydrochloride. MS m / z=631.3 [M+H] + .

[0625] Example 20 [ka]

[0626] Step 1: Synthesis of compound 20-2

[0627] Compound 20-1 (480g, 2.53mol) was weighed out. DMF (2500mL), 4-methoxybenzyl chloride (5.18mol, 702.79mL), potassium carbonate (872.82g, 6.32mol) and potassium iodide (419.35g, 2.53mol) were added. The mixture was reacted at 65°C for 2 hours. Water (1000mL) was added. The mixture was extracted with ethyl acetate (1000mL*3). The organic phase was concentrated under reduced pressure to give compound 20-2. MS m / z=430.0[M+H] + .

[0628] Step 2: Synthesis of compound 20-3

[0629] Compound 2,2,6,6-tetramethylpiperidine (220.59g, 1.56mol, 265.13mL) was weighed out. THF (3000mL) was added and n-butyllithium (2.5M, 499.73mL) was added at -5℃. The mixture was stirred for 0.5h. The mixture was cooled to -60℃. 20-2 (280g, 624.67mmol) was added to the mixture. The mixture was stirred for 0.5h. Finally, DMF (228.28g, 3.12mol, 240.30mL) was added. The mixture was reacted for another 0.5h. The reaction solution was poured into water (1000mL). The mixture was adjusted to pH 7 by adding 1N hydrochloric acid. The mixture was extracted with ethyl acetate (1000mL*3). The filtrate was concentrated under reduced pressure and purified by column chromatography (petroleum ether:ethyl acetate=10:1) to give compound 20-3.

[0630] Step 3: Synthesis of compound 20-4

[0631] 20-3 (370 g, 807.30 mmol) was weighed out. Toluene (1500 mL), dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium (2.86 g, 4.04 mmol, 2.86 mL) and tributyl(1-propynyl)tin (265.69 g, 807.30 mmol) were added. The mixture was reacted at 120° C. for 2 hours under nitrogen. The mixture was concentrated under reduced pressure and purified by column chromatography (petroleum ether:ethyl acetate=5:1) to give compound 20-4. MS m / z=418.1[M+H] + .

[0632] Step 4: Synthesis of compound 20-5

[0633] 20-4 (450 g, 970.13 mmol) was weighed out. DMF (100 mL) and N-bromosuccinimide (189.93 g, 1.07 mol) were added. The mixture was reacted at 25° C. for 2 h. Additional N-bromosuccinimide (17.27 g, 97.01 mmol) was added. The mixture was reacted for another 3 h. The reaction solution was concentrated under reduced pressure and purified by column chromatography (petroleum ether:ethyl acetate=5:1) to give compound 20-5. MS m / z=496.0[M+H] + .

[0634] Step 5: Synthesis of compound 20-6

[0635] 20-5 (55 g, 110.81 mmol) was weighed. DMF (300 mL), methyl fluorosulfonyldifluoroacetate (42.57 g, 221.61 mmol, 28.19 mL) and copper(I) iodide (42.21 g, 221.61 mmol) were added. The mixture was reacted at 110° C. for 2 h under nitrogen. The reaction was quenched by adding 500 mL of water. The mixture was extracted with ethyl acetate (600 mL*3). The organic phases were combined. The combined organic phases were washed successively with water (800 mL*2) and saturated brine (800 mL), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated. The residue was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to give compound 20-6. MS m / z=485.9[M+H] + .

[0636] Step 6: Synthesis of compound 20-7

[0637] Methyl acetoacetate (18.42 g, 158.61 mmol, 17.10 mL) was added dropwise to a solution of sodium hydride (6.34 g, 158.61 mmol, 60% purity) in tetrahydrofuran (350 mL) at 0°C. The mixture was allowed to react for 15 min. A solution of 20-6 (35 g, 72.10 mmol) in tetrahydrofuran (350 mL) was added. The mixture was allowed to react for 0.5 h. The reaction was quenched by adding 200 mL of saturated ammonium chloride solution. The mixture was extracted with ethyl acetate (300 mL*2). The organic phases were combined, washed with saturated brine (400 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure. The residue was purified by column chromatography (petroleum ether:ethyl acetate=10:1 to 1:1) to give compound 20-7. MS m / z=624.2[M+Na] + .

[0638] Step 7: Synthesis of compound 20-8

[0639] 20-7 (38 g, 63.17 mmol) was weighed. Dichloromethane (300 mL) and N,N-dimethylformamide dimethyl acetal (9.03 g, 75.80 mmol) were added. The mixture was reacted at 25 °C for 16 h. The reaction solution was cooled to 0 °C. Boron trifluoride diethyl etherate (10.76 g, 75.80 mmol, 9.32 mL) was added. The mixture was reacted at 0 °C for another 1 h. 200 mL of saturated sodium bicarbonate solution was added to the reaction system. The organic phase was separated. The aqueous phase was extracted with 200 mL of dichloromethane. The extracted organic phases were combined. The combined organic phase was washed with 250 mL of saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure. The residue was purified by column chromatography (petroleum ether: ethyl acetate = 10: 1 to 1: 1) to give compound 20-8. MS m / z=612.1[M+H] + .

[0640] Step 8: Synthesis of compound 20-9

[0641] 20-8 (30 g, 49.05 mmol) was weighed. Tetrahydrofuran (300 mL) was added. Tri-sec-butyllithium borohydride (1 M, 53.96 mL) was added to the mixture at -60°C. The mixture was reacted at -60°C for 1 h. The reaction was quenched by adding 200 mL of water. The mixture was extracted with ethyl acetate (300 mL*2). The organic phases were combined, washed with 300 mL of saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure. The residue was purified by column chromatography (petroleum ether:ethyl acetate=10:1-5:1) to give compound 20-9. MS m / z=614.1[M+H] + .

[0642] Step 9: Synthesis of compound 20-10

[0643] 20-9 (20g, 32.59mmol) was weighed. Ethanol (200mL), 2-methyl-2-thioisourea sulfate (27.22g, 97.78mmol) and sodium carbonate (6.91g, 65.19mmol) were added. The mixture was reacted at 50°C for 13 hours. The reaction solution was concentrated to dryness. 40mL of water was added. The mixture was extracted with ethyl acetate (50mL*2). The organic phases were combined. The combined organic phases were washed with 60mL of saturated brine, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give compound 20-10. MS m / z=654.3[M+H] + .

[0644] Step 10: Synthesis of compound 20-11B

[0645] 20-10 (21 g, 32.13 mmol) was weighed. DMF (2.00 mL), N,N-diisopropylethylamine (12.46 g, 96.38 mmol, 16.79 mL) and N-phenylbis(trifluoromethanesulfonyl)imide (13.77 g, 38.55 mmol) were added. The mixture was reacted at 25°C for 1 hour. 300 mL of water was added to the system. The mixture was extracted with ethyl acetate (300 mL*3). The organic phase was washed with water (400 mL*2) and saturated brine (400 mL) successively, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure and purified by column chromatography (petroleum ether:ethyl acetate=10:1) to give compound 20-11. This was separated by SFC (chromatography column: DAICEL CHIRALPAK IG (250mm*50mm, 10μm); mobile phase: [supercritical CO2-ethanol (0.1% ammonia water)]; ethanol%: 25%-25%) to obtain compound 20-11A and compound 20-11B. Chiral SFC analysis (chromatography column: ChiralPak IG-3 (100mm*4.6mm, 3μm); mobile phase: [supercritical CO2-methanol (0.05% diethylamine)]; (ethanol (0.05% diethylamine))%: 5%-40%), compound 20-11A (Rt=2.574min, ee value 99%); compound 20-11B (Rt=3.055min, ee value 99%).

[0646] Step 11: Synthesis of compound 20-12

[0647] Compound 20-11B (2 g, 2.55 mmol) and 20-12A (871.78 mg, 3.82 mmol) were weighed and dissolved in DMF (10 mL). N,N-diisopropylethylamine (986.88 mg, 7.64 mmol) was added. The mixture was reacted at 100° C. for 1 h. The reaction solution was concentrated under reduced pressure and purified by column chromatography (petroleum ether:ethyl acetate=5:1) to obtain compound 20-12. MS m / z=864.0[M+H] + .

[0648] Step 12: Synthesis of compound 20-13

[0649] Compound 20-12 (1.8 g, 2.08 mmol) was weighed and dissolved in THF (20 mL), and metachloroperbenzoic acid (422.96 mg, 2.08 mmol, 85% purity) was added. The mixture was stirred at 25° C. for 0.5 h. The reaction solution was concentrated under reduced pressure to give compound 20-13. MS m / z=880.0 [M+H] + .

[0650] Step 13: Synthesis of compound 20-14

[0651] Compound 8-2 (626.81 mg, 4.09 mmol) was weighed and dissolved in THF (5 mL). Sodium tert-butoxide (393.15 mg, 4.09 mmol) was added at 0°C. After 1 h, 20-13 (1.8 g, 2.05 mmol) was added. The mixture was reacted for another 1 h. The reaction was quenched by adding 10 mL of water. The mixture was adjusted to pH 6 with 1N dilute hydrochloric acid. The mixture was extracted with ethyl acetate (100 mL*2). The organic phases were combined, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure and purified by column chromatography (dichloromethane:methanol=10:1) to give compound 20-14. MS m / z=969.1[M+H] + .

[0652] Step 14: Synthesis of Compound 20 Hydrochloride

[0653] Compound 20-14 (1.4 g, 1.44 mmol) was weighed and dissolved in trifluoroacetic acid (10 mL). The mixture was reacted at 25° C. for 1 hour. The reaction solution was concentrated under reduced pressure, and the crude product was purified by high performance liquid chromatography (column: Xtimate C18 150*40 mm*5 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; (acetonitrile)%: 5%-35%, 10 min) to obtain compound 20 hydrochloride. MS m / z=610.6[M+H] + .

[0654] Example 21 [ka]

[0655] Step 1: Synthesis of intermediate 21-1

[0656] Compound 20-3 (19 g, 41.46 mmol) and pentanoic acid (4.88 g, 49.75 mmol) were added to dimethyl sulfoxide (200 mL). Dichlorobistriphenylphosphinepalladium (290.98 mg, 414.56 μmol), 1,4-bis(diphenylphosphino)butane (353.59 mg, 829.12 μmol) and 1,8-diazabicyclo[5.4.0]undecane-7-ene (18.93 g, 124.37 mmol, 18.75 mL) were added. The atmosphere was replaced with nitrogen. The resulting mixture was heated to 110-115 °C and reacted with stirring for 18 hours. The reaction solution was diluted with 500 mL of ethyl acetate and washed with water (50 mL × 3) and saturated brine (100 mL). The organic phase was concentrated under reduced pressure to obtain the crude product. This was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to obtain compound 21-1. 1 H NMR(400MHz,CDCl3)δ:10.34(s,1H),7.42(dd,J=2.0,5.6Hz,1H),7.16(d,J=8.4Hz,4H),7.11-7.06(m ,1H),6.87-6.82(m,4H),4.24(s,4H),3.82-3.78(m,6H),2.37(q,J=7.6Hz,2H),1.21(t,J=7.6Hz,3H).

[0657] Step 2: Synthesis of intermediate 21-2

[0658] Compound 21-1 (4.0 g, 9.27 mmol) was dissolved in DMF (30 mL). N-bromosuccinimide (2.14 g, 12.05 mmol) was added. The resulting reaction solution was stirred under nitrogen at 50° C. for 3 h. The reaction solution was diluted with 150 mL of ethyl acetate and then washed with water (20 mL×3) and saturated brine (20 mL). The organic phase was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to obtain compound 21-2. MS m / z=510.1 [M+H] + .

[0659] Step 3: Synthesis of intermediate 21-3

[0660] Compound 21-2 (4.6 g, 9.01 mmol) and compound fluorosulfonyl difluoroacetate methyl (6.93 g, 36.05 mmol, 4.59 mL) were added to N,N-dimethylformamide (30 mL). Copper iodide (3.43 g, 18.03 mmol) was added. The resulting reaction solution was heated to 105° C. under nitrogen and stirred for 3 hours. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column (petroleum ether:ethyl acetate=5:1) to obtain compound 21-3. MS m / z=500.0[M+H] + .

[0661] Step 4: Synthesis of intermediate 21-4

[0662] Sodium hydride (856.78 mg, 21.42 mmol, 60% purity) was suspended in anhydrous tetrahydrofuran (30 mL). The resulting mixture was cooled to 0° C. Then, methyl acetoacetate (2.09 g, 17.99 mmol, 1.94 mL) was added dropwise. After the addition, the mixture was stirred for 30 min, and then n-butyllithium (2.5 M, 7.88 mL) was added dropwise. After the addition, the mixture was stirred for another 30 min. The ice bath was removed. The mixture was cooled to −78° C. Finally, a solution of compound 21-3 (4.28 g, 8.57 mmol) in tetrahydrofuran (15 mL) was added dropwise to the mixture. After the addition, the mixture was stirred for 0.5 h. The reaction was quenched with 1 M hydrochloric acid. The mixture was adjusted to pH 5 and the organic phase was separated. The aqueous phase was extracted three times with 30 mL of ethyl acetate. The organic phases were combined and concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=2:1) ​​to give compound 21-4. MS m / z=638.2[M+Na] + .

[0663] Step 5: Synthesis of intermediate 21-5

[0664] Compound 21-4 (5.0 g, 6.01 mmol) was dissolved in dichloromethane (40 mL) and then N,N-dimethylformamide dimethyl acetal (2.15 g, 18.03 mmol, 2.40 mL) was added. The resulting reaction solution was stirred under nitrogen for 18 hours. The reaction solution was cooled to 0° C. and boron trifluoride diethyl etherate (853.03 mg, 6.01 mmol) was added dropwise. After the addition was complete, the reaction solution was stirred for 1 hour. The reaction was quenched with 20 mL of saturated sodium bicarbonate. The mixture was then extracted three times with 30 mL of dichloromethane. The organic phases were combined and concentrated under reduced pressure to give the crude product. The crude product was purified by column (petroleum ether:ethyl acetate=2:1) ​​to give compound 21-5. MS m / z=626.0[M+H] + .

[0665] Step 6: Synthesis of intermediate 21-6

[0666] Compound 21-5 (2.6 g, 4.16 mmol) was dissolved in anhydrous tetrahydrofuran (20 mL). The mixture was cooled to -78 °C under nitrogen, and then tri-sec-butyllithium borohydride (1 M, 4.57 mL) was added dropwise. After the addition was complete, the reaction solution was stirred at -78 °C for 0.5 h. The reaction was quenched by adding 15 mL of water. The mixture was adjusted to pH 5-6 with 1 M hydrochloric acid, and the organic phase was separated. The aqueous layer was extracted with 30 mL x 3. The organic phases were combined and concentrated under reduced pressure to give the crude product. The crude product was purified by column (petroleum ether: ethyl acetate = 5:1) to give compound 21-6. MS m / z = 650.0 [M + Na] + .

[0667] Step 7: Synthesis of intermediate 21-7

[0668] Compound 21-6 (0.6 g, 955.99 μmol) and 2-methylthiourea sulfate (539.83 mg, 2.87 mmol) were added to ethanol (15 mL), followed by the addition of sodium carbonate (202.65 mg, 1.91 mmol). The resulting reaction solution was heated to 65° C. under nitrogen and stirred for 18 hours. The reaction solution was concentrated under reduced pressure. To the residue was added 10 mL of water and 80 mL of ethyl acetate. The mixture was adjusted to pH 6 with 2M hydrochloric acid while stirring. The aqueous layer was separated and the organic phase was washed with 10 mL of saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure to give compound 21-7. MS m / z=668.3 [M+H] + .

[0669] Step 8: Synthesis of intermediate 21-8

[0670] Compound 21-7 (640 mg, 958.50 μmol) and compound N-phenylbis(trifluoromethanesulfonyl)imide (513.63 mg, 1.44 mmol) were added to N,N-dimethylformamide (5 mL), followed by the addition of N,N-diisopropylethylamine (247.76 mg, 1.92 mmol). The atmosphere was replaced with nitrogen. The reaction solution was heated to 35° C. and stirred for 1.5 hours. The reaction solution was diluted with 50 mL of ethyl acetate and then washed with water (10 mL×3). The organic phase was concentrated under reduced pressure to obtain a crude product. This was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to obtain compound 21-8. MS m / z=800.4[M+H] + .

[0671] Step 9: Synthesis of intermediate 21-9

[0672] Compound 21-8 (340 mg, 425.12 μmol) and compound 1-1A (180.50 mg, 850.24 μmol) were added to N,N-dimethylformamide (3 mL), followed by the addition of N,N-diisopropylethylamine (164.83 mg, 1.28 mmol). The resulting reaction solution was heated to 105° C. under nitrogen and stirred for 1 hour. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=2:1) ​​to obtain compound 21-9. MS m / z=862.4[M+H] + .

[0673] Step 10: Synthesis of intermediate 21-10

[0674] Compound 21-9 (223 mg, 258.71 μmol) was dissolved in dichloromethane (3 mL), and then metachloroperbenzoic acid (52.52 mg, 258.71 μmol, 85% purity) was added. The resulting reaction solution was stirred at 25° C. for 1 h under nitrogen. The reaction solution was concentrated under reduced pressure to obtain the crude product, which was purified by column chromatography (petroleum ether:ethyl acetate=1:1) to obtain compound 21-10. MS m / z=894.4[M+H] + .

[0675] Step 11: Synthesis of intermediate 21-11

[0676] Compound 8-2 (76.44 mg, 498.89 μmol) and sodium tert-butoxide (47.95 mg, 498.89 μmol) were added to tetrahydrofuran (2 mL). The mixture was stirred at 25° C. for 0.5 h. A solution of compound 21-10 (223 mg, 249.45 μmol) in tetrahydrofuran (2 mL) was added dropwise to the reaction solution. The mixture was stirred at 25° C. for 0.5 h. The reaction solution was diluted with 30 mL of ethyl acetate and then washed with 5 mL of saturated brine. The organic phase was dried over anhydrous sodium sulfate and concentrated under reduced pressure to give compound 21-11. MS m / z=967.8 [M+H] + .

[0677] Step 12: Synthesis of Compound 21

[0678] Compound 21-11 (238 mg, 246.10 μmol) was added to trifluoroacetic acid (2 mL). The reaction was stirred at 25° C. for 1 h. The reaction solution was concentrated under reduced pressure to give the crude product, which was purified by HPLC (column: Xtimate C18 150*40 mm*5 μm; mobile phase: [water (0.05% hydrochloric acid)-acetonitrile]; (acetonitrile)%: 5%-35%, 10 min) to give compound 21 hydrochloride. MS m / z=627.4[M+H] + .

[0679] Step 13: Synthesis of Compounds 21A and 21B

[0680] Compound 21 hydrochloride was separated by SFC (chromatographic column: DAICEL CHIRALPAK IC (250 mm * 30 mm, 10 μm); mobile phase: [supercritical CO2-methanol (0.1% ammonia water)]; methanol %: 60%-60%) to obtain compounds 21A and 21B. Chiral SFC analysis (chromatographic column: Chiral Pak IC (100 mm * 4.6 mm, 3 μm); mobile phase: [supercritical CO2-ethanol (0.05% diethylamine)]; (methanol (0.05% diethylamine)) %: 60%-60%). Compound 21A (Rt = 1.696 min, ee value 99%, MS m / z = 627.4 [M + H] + ); 1 H NMR(400MHz,CDCl3)δ:6.89(d,J=8.4Hz,1H),5.21(d,J=12.0Hz,2H),5.11(d,J=7.6H z,1H),4.80-4.50(m,4H),4.44-4.27(m,2H),4.23-3.82(m,5H),3.77-3.48(m,3H),3 .35-3.20(m,1H),3.11-2.86(m,3H),2.63-2.51(m,1H),2.38(q,J=7.2Hz,3H),2.30- 2.06(m,5H),2.04-1.75(m,2H),1.20(t,J=7.6Hz,3H).Compound 21B (Rt=5.728 min, ee value 99%, MS m / z=627.3[M+H] + ), 1 H NMR(400MHz,CDCl3)δ:6.86(d,J=8.4Hz,1H),5.19-5.08(m,1H),4.95(br s,2H),4.80-4.64(m,3H),4.15-4.03(m,4H),4.01-3.93(m,1H),3.82- 3.67(m,3H),3.59-3.43(m,2H),3.36-3.14(m,4H),3.00-2.90(m,1H), 2.80(d,J=15.0Hz,1H),2.72-2.62(m,1H),2.45-2.32(m,3H),2.22-2. 11(m,1H),2.09-1.84(m,5H),1.82-1.67(m,2H),1.21(t,J=7.2Hz,3H).

[0681] Example 22 [ka]

[0682] Step 1: Synthesis of intermediate 22-1 formate salt

[0683] Compound 16-1 (1.28 g, 6.56 mmol) was dissolved in toluene (10.0 mL). Isobutylene / tetrahydrofuran solution (2.40 M, 2.73 mL) and Hoveyda-Grubbs catalyst (411 mg, 656 μmol) were added. The mixture was reacted at 120° C. for 12 hours. The reaction solution was filtered using a Celite® pad, and the filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.225% formic acid)-acetonitrile]; acetonitrile: 25%-55%) to obtain compound 22-1. MS m / z=224.1[M+1] + .

[0684] Step 2: Synthesis of intermediate 22-2

[0685] Compound 22-1 (200 mg) was dissolved in THF (2.00 mL). The mixture was cooled to 0° C., and red aluminum / toluene solution (1.03 g, 3.58 mmol, 70.0% purity) was slowly added dropwise at 0° C. After the addition was completed, the reaction solution was reacted at 25° C. for 12 hours. Methanol (3.00 mL) was added to the reaction solution, and the mixture was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (dichloromethane:methanol=10:1) to obtain compound 22-2. MS m / z=182.1[M+1] + .

[0686] Step 3: Synthesis of intermediate 22-3 formate salt

[0687] Compound 1-12B (100 mg, 116 μmol) was dissolved in toluene (1.00 mL), and compound 22-2 (105 mg, 581 μmol), 4A molecular sieves (100 mg) and sodium tert-butoxide (33.5 mg, 349 μmol) were added to the solution. The reaction solution was reacted at 110° C. for 12 hours. The reaction solution was cooled and filtered using a Celite® pad. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.225% formic acid)-acetonitrile]; acetonitrile: 50%-80%) to obtain compound 22-3 formate salt. MS m / z=977.3[M+1] + .

[0688] Step 4: Synthesis of intermediate 22-4

[0689] Compound 22-3 formate (45.0 mg, 46.0 μmol) was dissolved in toluene (1.00 mL), and 1-propynyltri-n-butyltin (60.6 mg, 184 μmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (9.78 mg, 13.8 μmol) were added to the reaction solution. The reaction solution was reacted at 110° C. for 3 hours. The reaction solution was cooled and filtered using a Celite® pad. The filtrate was concentrated under reduced pressure to obtain a crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile: 52%-82%, 8 min) to obtain compound 22-4 trifluoroacetate. MS m / z=981.4[M+1] + .

[0690] Step 5: Synthesis of compound 22 formate salt

[0691] Compound 22-4 trifluoroacetate (35.0 mg, 35.7 μmol) was dissolved in dichloromethane (1.00 mL), and trifluoroacetic acid (0.20 mL) was added to the reaction solution. The reaction solution was reacted at 25° C. for 1 hour, and then catalytically concentrated under reduced pressure to obtain a crude product. The crude product was purified sequentially by HPLC (column: Phenomenex luna C18 150*25mm*10μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile: 20%-50% over 9 min); (column: Waters Xbridge 150*25mm*5μm; mobile phase: [water (0.1% ammonia)-acetonitrile]; acetonitrile: 55%-85% over 10 min); (column: Phenomenex luna C18 150*25mm*10μm; mobile phase: [water (0.225% formic acid)-acetonitrile]; acetonitrile: 15%-45% over 9 min) to obtain compound 22 formate. MS m / z=641.3[M+1] + .

[0692] Example 23 [ka]

[0693] Step 1: Synthesis of intermediate 23-2

[0694] Compound 23-1 (5.5 g) was dissolved in DMF (50 mL), and potassium carbonate (15.8 g, 114 mmol) and compound allyl bromide (4.17 g, 34.4 mmol) were added. The mixture was reacted at room temperature for 12 hours. 100 mL of water was poured into the reaction solution. The mixture was extracted with ethyl acetate (200 mL) three times. The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=20:1) to obtain compound 23-2.

[0695] Step 2: Synthesis of intermediate 23-3

[0696] Compound 23-2 (5.5 g, 28.1 mmol) was dissolved in anhydrous THF (37.0 mL) and lithium aluminum hydride (2.5 M, 13.5 mL) was added at 0° C. The mixture was reacted at room temperature for 1 h. 1.2 mL of water, 1.2 mL of 15% aqueous NaOH solution, and finally 3.6 mL of water were added to the reaction solution. The organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give the crude product, which was used directly in the next step. Compound 23-3 was obtained.

[0697] Step 3: Synthesis of intermediate 23-4

[0698] Compound 23-3 (2.5 g) was dissolved in anhydrous DCM (20.0 mL). Imidazole (4.07 g, 59.7 mmol) and 4-dimethylaminopyridine (182 mg, 1.49 mmol) were added, followed by tert-butyldiphenylsilyl chloride (8.22 g, 29.9 mmol) at 0° C. The mixture was reacted at room temperature for 12 hours. The reaction was quenched by adding 100 mL of water to the reaction solution, and the mixture was extracted three times with DCM (100 mL). The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to give compound 23-4. MS m / z=406.2[M+H] + .

[0699] Step 4: Synthesis of intermediate 23-5

[0700] Compound 23-4 (1.5 g, 3.70 mmol) was dissolved in anhydrous toluene (30 mL) and Grubbs' catalyst (313.93 mg, 369.78 μmol) was added. The mixture was reacted at 120° C. for 12 hours. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by thin plate chromatography (dichloromethane:methanol=10:1) to obtain compound 23-5. MS m / z=378.2 [M+H] + .

[0701] Step 5: Synthesis of intermediate 23-6 hydrochloride

[0702] Compound 23-5 (200 mg, 529 μmol) was dissolved in anhydrous dioxane (6.0 mL) and hydrochloric acid (12 M, 44.1 μL) was added. The mixture was reacted at 95° C. for 12 hours. 10 mL of water was added to the reaction solution. The mixture was extracted. The aqueous phase was lyophilized to obtain a crude product that can be used directly in the next step. Compound 23-6 hydrochloride was obtained.

[0703] Step 6: Synthesis of intermediate 23-7

[0704] Compound 23-6 hydrochloride (207 mg) was dissolved in anhydrous toluene (3 mL), and sodium tert-butoxide (250 mg, 2.60 mmol) and compound 1-12B (320 mg, 371 μmol) were added. The mixture was reacted at room temperature for 12 hours. 30 mL of saturated aqueous ammonium chloride solution was poured into the reaction solution. The mixture was extracted with ethyl acetate (30 mL). The organic phase was washed with saturated brine (20 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (dichloromethane:methanol=10:1) to obtain compound 23-7. MS m / z=935.3[M+H] + .

[0705] Step 7: Synthesis of intermediate 23-8

[0706] Compound 23-7 (130 mg, 138 μmol) was dissolved in anhydrous toluene (1 mL), and 1-propynyltri-n-butyltin (182 mg, 555 μmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (9.84 mg, 13.9 μmol) were added. The atmosphere was replaced with nitrogen three times. The mixture was reacted under nitrogen at 130° C. for 4 hours. The reaction solution was cooled and concentrated under reduced pressure to obtain the crude product. The crude product was purified by thin plate chromatography (dichloromethane:methanol=10:1) to obtain compound 23-8. MS m / z=939.4[M+H] + .

[0707] Step 8: Synthesis of Compound 23 Trifluoroacetate

[0708] Compound 23-8 (100 mg, 106 μmol) was dissolved in dichloromethane (1.0 mL). Trifluoroacetic acid (182 mg, 1.60 mmol) was added. The mixture was reacted at 25° C. for 0.5 hours. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile: 10%-40% over 9 min) and lyophilized to obtain compound 23 trifluoroacetate. MS m / z=599.3[M+H] + . 1 H NMR(400MHz,CD3OD)6.92(d,J=8.4Hz,1H),6.00(d,J=6.4Hz,1H),5.87(td,J=2.4,6.4Hz,1H),5.18(dd,J=4.4,11 .2Hz,1H),4.88-4.85(m,1H),4.76-4.70(m,2H),4.61-4.52(m,2H),4.36-4.28(m,1H),4.20-4.11(m,2H),4.04(br d,J=16.0Hz,1H),3.85-3.74(m,2H),3.66(br d,J=13.6Hz,1H),3.35(br d,J=6.4Hz,2H),3.29-3.24(m,1H),2.92(br dd,J=3.6,18.0Hz,1H),2.31-2.09(m,7H),2.04-1.95(m,4H).

[0709] Example 24 [ka]

[0710] Step 1: Synthesis of intermediate 24-2

[0711] Compound 24-1 (54 g, 198 mmol) was dissolved in anhydrous THF (450 mL). The mixture was cooled to -78°C, and methylmagnesium bromide (3 M, 79.2 mL) was added dropwise. The mixture was reacted at -78°C for 0.5 h, then at room temperature for 12 h. The reaction solution was quenched by adding 200 mL of hydrochloric acid (1 M). The mixture was extracted three times with ethyl acetate (200 mL). The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to give compound 24-2. MS m / z=288.0[M+1] + .

[0712] Step 2: Synthesis of intermediate 24-3

[0713] Compound 24-2 (38 g, 131 mmol) was dissolved in anhydrous DCM (380 mL). Dess-Martin oxidant (111 g, 263 mmol, 81.6 mL) was added. The mixture was reacted at room temperature for 4 hours. 200 mL of water was poured into the reaction solution. The mixture was extracted three times with ethyl acetate (200 mL). The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to give compound 24-3. MS m / z=285.9[M+1] + .

[0714] Step 3: Synthesis of intermediate 24-4

[0715] Methyltriphenylphosphine bromide (8.42 g, 23.5 mmol) was dissolved in anhydrous tetrahydrofuran (45 mL). The mixture was cooled to -78 °C. n-Butyl lithium (2.5 M, 9.42 mL) was added dropwise. The mixture was reacted at 0 °C for 2 h. Then the mixture was cooled to -78 °C. 24-3 (4.5 g, 15.7 mmol) dissolved in THF (20 mL) was added dropwise. The mixture was reacted at room temperature for 10 h. 100 mL of aqueous HCl (1 M) was poured into the reaction solution, and the mixture was extracted three times with EA (100 mL). The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether: ethyl acetate = 10: 1) to give compound 24-4.

[0716] Step 4: Synthesis of intermediate 24-5 hydrochloride

[0717] Compound 24-4 (5.5 g, 19.3 mmol) was dissolved in a solution of hydrochloric acid in methanol (4 M, 61.1 mL). The mixture was reacted at room temperature for 12 hours. The reaction solution was concentrated under reduced pressure to give compound 24-5 hydrochloride. MS m / z=170.1 [M+1] + .

[0718] Step 5: Synthesis of intermediate 24-6

[0719] Compound 24-5 hydrochloride (4.5 g) was dissolved in DMF (40 mL), and potassium carbonate (14.7 g, 106 mmol) and allyl bromide (3.86 g, 31.9 mmol) were added. The mixture was reacted at room temperature for 1.5 hours. 100 mL of water was poured into the reaction solution, and the mixture was extracted three times with ethyl acetate (200 mL). The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to obtain compound 24-6.

[0720] Step 6: Synthesis of intermediate 24-7

[0721] Compound 24-6 (2.5 g, 11.9 mmol) was dissolved in anhydrous tetrahydrofuran (20.0 mL) and lithium aluminum hydride (2.5 M, 6.32 mL) was added at 0° C. The mixture was reacted at room temperature for 1 hour. 0.6 mL of water, 0.6 mL of 15% aqueous sodium hydroxide solution, and finally 1.8 mL of water were added to the reaction solution. The mixture was dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give crude compound 24-7. MS m / z=182.1 [M+H] + .

[0722] Step 7: Synthesis of intermediate 24-8

[0723] Compound 24-7 (2.3 g) was dissolved in anhydrous dichloromethane (23.0 mL). Imidazole (3.46 g, 50.7 mmol), 4-dimethylaminopyridine (155 mg, 1.27 mmol) were added, followed by tert-butyldiphenylsilyl chloride (6.97 g, 25.3 mmol) at 0° C. The mixture was reacted at room temperature for 12 hours. 50 mL of water was poured into the reaction solution. The mixture was extracted three times with ethyl acetate (100 mL). The organic phase was washed with saturated brine (10 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to obtain compound 24-8. MS m / z=420.3[M+H] + .

[0724] Step 8: Synthesis of intermediate 24-9

[0725] Compound 24-8 (2.5 g, 5.96 mmol) was dissolved in anhydrous toluene (50 mL) and Grubbs' catalyst (1.01 g, 1.19 mmol) was added. The mixture was reacted at 120° C. for 12 hours. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (dichloromethane:methanol=20:1) to obtain compound 24-9. MS m / z=392.2[M+H] + .

[0726] Step 9: Synthesis of intermediate 24-10

[0727] Compound 24-9 (1.00 g, 2.55 mmol) was dissolved in anhydrous dioxane (6.0 mL) and hydrochloric acid (12 M, 212 μL) was added. The mixture was reacted at 95° C. for 12 hours. 10 mL of water was added to the reaction solution. The mixture was extracted with ethyl acetate (20 mL*3). The aqueous phase was lyophilized to give crude compound 24-10.

[0728] Step 10: Synthesis of intermediate 24-11

[0729] Compound 24-10 (92.6 mg) was dissolved in anhydrous toluene (3 mL). Sodium tert-butoxide (101 mg, 1.06 mmol) and compound 1-12B (130 mg, 151 μmol) were added. The mixture was reacted at 95° C. for 12 hours. The reaction solution was concentrated under reduced pressure to obtain a crude product. The crude product was purified by column chromatography (dichloromethane:methanol=10:1) to obtain compound 24-11.

[0730] Step 11: Synthesis of intermediate 24-12

[0731] Compound 24-11 (89 mg, 93.7 μmol) was dissolved in anhydrous toluene (1 mL), and 1-propynyltri-n-butyltin (123 mg, 374 μmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (6.64 mg, 9.37 μmol) were added. The atmosphere was replaced with nitrogen three times. The mixture was reacted under nitrogen at 130° C. for 0.5 hours. The reaction solution was cooled and concentrated under reduced pressure to obtain the crude product. The crude product was purified by thin plate chromatography (dichloromethane:methanol=10:1) to obtain compound 24-12.

[0732] Step 12: Synthesis of Compound 24 Trifluoroacetate

[0733] Compound 24-12 (90 mg, 94.4 μmol) was dissolved in dichloromethane (1.0 mL). Trifluoroacetic acid (161 mg, 1.42 mmol) was added. The mixture was reacted at 25° C. for 1.5 hours. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile: 20%-50% over 7 min) and lyophilized to obtain compound 24 trifluoroacetate. MS m / z=613.2 [M+H] + . 1 H NMR(400MHz,METHANOL-d4)6.92(d,J=8.2Hz,1H),5.61(d,J=1.4Hz,1H),5.18(br dd,J=4.4,11.6Hz,1H),4.91-4.89(m,2H),4.86-4.82(m,1H),4.80-4.70(m,2H),4.51-4.41(m,2H),4.29(br d,J=14.0Hz,1H),4.19-4.10(m,2H),3.91(br d,J=15.2Hz,1H),3.85-3.74(m,2H),3.63(br d,J=14.4Hz,1H),3.27(br d,J=5.2Hz,2H),2.95(br d,J=1.2Hz,1H),2.28-2.08(m,7H),2.02(s,3H),1.83(d,J=1.6Hz,3H).

[0734] Example 25 [ka]

[0735] Step 1: Synthesis of intermediate 25-2

[0736] Compound 25-1 (9.80 g, 43.5 mmol) was dissolved in anhydrous DMF (98 mL), and then p-methoxybenzyl chloride (8.17 g, 52.2 mmol), potassium iodide (7.22 g, 43.5 mmol) and potassium carbonate (15.0 g, 109 mmol) were added. The mixture was reacted at 50° C. for 12 hours. 50.0 mL of water was poured into the reaction solution, and the mixture was extracted with ethyl acetate (150 mL). The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to obtain compound 25-2. MS m / z=345.6[M+H] + .

[0737] Step 2: Synthesis of intermediate 25-3

[0738] 2,2,6,6-Tetramethylpiperidine (21.3 g, 150 mmol) was dissolved in tetrahydrofuran (130 mL) and the atmosphere was flushed with nitrogen three times. The mixture was cooled to -5°C, n-butyllithium (2.50 M, 60.2 mL) was added dropwise and stirred for 0.5 h. The mixture was then cooled to -60°C and compound 25-2 (13.0 g, 37.6 mmol) was added. The mixture was stirred for 0.5 h. DMF (55.0 g, 752 mmol) was then added at -60°C and the mixture was stirred for 0.5 h. The reaction was quenched by adding 200 mL of saturated ammonium chloride solution at 0°C. The mixture was extracted with ethyl acetate (300 mL). The organic phase was washed with saturated brine (300 mL), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=50:1) to give compound 25-3. MS m / z=373.28[M+H] + .

[0739] Step 3: Synthesis of intermediate 25-4

[0740] Compound 25-3 (4.9 g, 13.12 mmol), copper iodide (5.00 g, 26.23 mmol) and methyl 2,2-difluoro-2-fluorosulfonyl acetate (9.57 g, 49.84 mmol) were dissolved in DMF (50 mL). The atmosphere was replaced with nitrogen three times. The mixture was reacted at 110° C. for 2.5 hours. The reaction solution was cooled and filtered through a Celite® pad. Water (100 mL) was added to the organic phase. The mixture was extracted once with ethyl acetate (100 mL). The organic phase was washed six times with saturated brine (100 mL), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=20:1) to obtain compound 25-4. 1 H NMR (CDCl3,400MHz)10.29(q,J=4.25Hz,1H),7.35(d,J=8.63Hz,2H),7.21(d,J=7.25Hz,1H),7.00-6.90(m,2H),5.14(s,2H),3.84(s,3H).

[0741] Step 4: Synthesis of intermediate 25-5

[0742] 2,2,6,6-Tetramethylpiperidine (3.68 g, 26.02 mmol, 4.42 mL) was dissolved in THF (30 mL). The atmosphere was replaced with nitrogen three times. The mixture was cooled to -40°C, and n-butyllithium (2.5 M, 10.06 mL) was slowly added dropwise. The mixture was reacted at -40°C for 0.5 h. The reaction system was cooled to -60°C. A solution of 3-8 (3.3 g, 8.67 mmol) in THF (10 mL) was slowly added dropwise to the above reaction solution. The mixture was warmed to -40°C and reacted for 15 min. Compound 25-4 (3.77 g, 10.41 mmol) was then added in portions to the above reaction solution, and the mixture was reacted at room temperature for 2 h. The reaction solution was quenched by adding saturated aqueous ammonium chloride solution (50 mL), and the mixture was extracted twice with ethyl acetate (50 mL). The organic phase was washed twice with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. A mixture of petroleum ether and ethyl acetate (petroleum ether:ethyl acetate=5:1) (20 mL) was added to the crude product, and the mixture was stirred for 12 hours. The solid was collected by filtration to give compound 25-5.

[0743] Step 5: Synthesis of intermediate 25-6

[0744] Compound 25-5 (2.7 g, 3.63 mmol) and cyanomethylenetributylphosphorane (1.75 g, 7.27 mmol) were dissolved in toluene (27 mL). The atmosphere was replaced with nitrogen. The mixture was reacted at 110° C. for 12 hours. 50 mL of aqueous ammonium chloride solution was poured into the reaction solution, and the mixture was extracted twice with ethyl acetate (50 mL). The organic phase was washed twice with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=10:1) to obtain compound 25-6. MS m / z=725.3[M+H] + .

[0745] Step 6: Synthesis of intermediate 25-7

[0746] Compound 25-6 (1.5 g, 2.07 mmol) was dissolved in anhydrous DCM (15.0 mL) and metachloroperbenzoic acid (461.92 mg, 2.28 mmol, 85% purity) was added in portions at 0° C. The mixture was reacted overnight at room temperature. The reaction solution was directly blended and purified by column chromatography (petroleum ether:ethyl acetate=1:1) to give compound 25-7. MS m / z=741.3 [M+H] + .

[0747] Step 7: Synthesis of intermediate 25-8

[0748] Compound 25-7 (1.9 g, 2.56 mmol) was dissolved in anhydrous toluene (19 mL), and compound 1-2A (816.20 mg, 5.13 mmol), sodium tert-butoxide (985.42 mg, 10.25 mmol) and 4A molecular sieves (1 g) were added. The atmosphere was replaced with nitrogen. The mixture was reacted at 100° C. for 12 hours. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=1:1) to obtain compound 25-8. MS m / z=836.5[M+H] + .

[0749] Step 8: Synthesis of intermediate 25-9

[0750] Compound 25-8 (1.50 g, 1.79 mmol) was dissolved in anhydrous toluene (15 mL), and 1-propynyltri-n-butyltin (2.36 g, 7.17 mmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (381.01 mg, 538.09 μmol) were added. The atmosphere was replaced with nitrogen three times. The mixture was reacted under nitrogen at 110° C. for 12 hours. The reaction solution was cooled and concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=1:1) to obtain compound 25-9. MS m / z=840.3[M+H] + .

[0751] Step 9: Synthesis of Compound 25

[0752] Compound 25-9 (1.27 g, 1.51 mmol) was dissolved in dichloromethane (13 mL). Trifluoroacetic acid (3.99 g, 35.00 mmol) was added. The mixture was reacted at 20° C. for 3 hours. The reaction solution was adjusted to pH 7 with saturated aqueous sodium bicarbonate. The mixture was concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Kromasil Eternity XT 250*80 mm*10 μm; mobile phase: [water (0.1% aqueous ammonia)-acetonitrile]; acetonitrile: 10%-40% over 20 min) and lyophilized to obtain compound 25. MS m / z=620.4 [M+H] + .

[0753] Step 10: Synthesis of Compounds 25A and 25B

[0754] Compound 25 was separated by SFC (column: DAICEL CHIRALPAK IC (250 mm*30 mm, 10 μm); mobile phase: [supercritical CO2-ethanol (0.1% ammonia)]; ethanol (0.1% aqueous ammonia)%: 50%-50%) to obtain compounds 25A and 25B.

[0755] Compound 25A: Chiral SFC analysis (chromatographic column: Chiral Pak IC-3 (100 mm*4.6 mm, 3 μm); mobile phase: [supercritical CO2-ethanol (0.05% diethylamine)]; (ethanol (0.05% diethylamine))%: 40%-40%), Rt=0.695 min, ee value 99%, MS m / z=620.2 [M+H] + ; 1 H NMR(CDCl3,400MHz)δ7.07(d,J=8.13Hz,1H),5.30-5.04(m,2H),4.78-4.59(m,2H),4.13-3.89(m,3H),3.56(br s, 2H), 3.45-2.77 (m, 12H), 2.29-2.05 (m, 3H), 2.01-1.57 (m, 8H).

[0756] Compound 25B: Chiral SFC analysis (chromatographic column: Chiral Pak IC-3 (100 mm*4.6 mm, 3 μm); mobile phase: [supercritical CO2-ethanol (0.05% diethylamine)]; (ethanol (0.05% diethylamine))%: 40%-40%), Rt=1.229 min, ee value 99%, MS m / z=620.2 [M+H] + . 1 H NMR(DMSO-d6,400MHz)9.44-8.91(m,2H),7.12(br d,J=8.25Hz,1H),5.68-5.45(m,1H),5.12(br dd,J=10.88,3.75Hz,1H),4.95-4.63(m,2H),4.45(s,2H),4.31-4.01(m,3H),3.95-3.51(m,6H),3.39-3.09(m,3H),2.99-2.81(m,1H),2.54(br d,J=4.38Hz,1H),2.44(br s,1H),2.35-2.24(m,1H),2.23-2.06(m,4H),1.99-1.87(m,2H),1.87-1.72(m,1H).

[0757] Example 26 [ka] [ka]

[0758] Step 1: Synthesis of intermediate 26-2

[0759] Compound 26-1 (5.00 g, 30.6 mmol) was dissolved in anhydrous dichloromethane (50.0 mL) and NBS (6.53 g, 36.7 mmol) was added. The mixture was reacted at room temperature for 1 hour. 50.0 mL of water was added to the reaction solution. The mixture was extracted with dichloromethane (150 mL). The organic phase was washed with saturated brine (150 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=20:1) to give compound 26-2. 1H NMR(400MHz,CDCl3)δ ppm7.14(dd,J=9.88,2.25Hz,1H)3.60-4.02(m,2H).

[0760] Step 2: Synthesis of intermediate 26-3

[0761] Compound 26-2 (5.60 g, 23.1 mmol) and p-methoxybenzyl chloride (8.68 g, 55.4 mmol) were dissolved in anhydrous DMF (56.0 mL), and potassium iodide (3.83 g, 23.1 mmol) and potassium carbonate (7.98 g, 57.7 mmol) were added. The mixture was reacted at 80° C. for 2 hours. 100 mL of water was poured into the reaction solution and extracted with ethyl acetate (150 mL). The organic phase was washed with saturated brine (100 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=20:1) to obtain compound 26-3. 1 H NMR(400MHz,CDCl3)δ ppm7.20(d,J=8.50Hz,4H)7.09(dd,J=10.38,2.13Hz,1H)6.83(d,J=8.63Hz,4H)4.16(s,4H)3.80(s,6H).

[0762] Step 3: Synthesis of intermediate 26-4

[0763] 2,2,6,6-Tetramethylpiperidine (10.3 g, 73.2 mmol, 12.4 mL) was dissolved in tetrahydrofuran (90.0 mL). The atmosphere was replaced with nitrogen three times. The mixture was cooled to -5°C and n-butyllithium (2.50 M, 29.3 mL) was added dropwise. The mixture was stirred for 0.5 h. The mixture was cooled to -60°C and compound 26-4 (9.20 g, 18.3 mmol) was added. The mixture was stirred for 1 h. Then DMF (26.8 g, 366 mmol) was added at -60°C. The mixture was stirred for another 0.5 h. 100 mL of saturated ammonium chloride solution was added at 0°C. The mixture was extracted with ethyl acetate (300 mL). The organic phase was washed with saturated brine (300 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=30:1) to give compound 26-4. MS m / z=510.8[M+H] + .

[0764] Step 4: Synthesis of intermediate 26-5

[0765] 2,2,6,6-Tetramethylpiperidine (3.56 g, 25.2 mmol, 4.28 mL) was dissolved in tetrahydrofuran (50.0 mL). The atmosphere was replaced with nitrogen three times. The mixture was cooled to -40°C and n-butyllithium (2.5 M, 9.76 mL) was added dropwise. The mixture was stirred for 0.5 h. The mixture was then cooled to -60°C and compound 3-8 (3.20 g, 8.41 mmol) was added. The mixture was stirred for 15 min. Compound 26-4 (5.37 g, 10.1 mmol) was then added at -60°C. The mixture was stirred at room temperature for another 0.5 h. 100 mL of saturated ammonium chloride solution was added at 25°C. The mixture was extracted with ethyl acetate (300 mL). The organic phase was washed with saturated brine (300 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 (250*70mm, 10μm); mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile: 80%-100% over 20 min) and lyophilized to give compound 26-5.

[0766] Step 5: Synthesis of intermediate 26-6

[0767] Compound 26-5 (2.20 g, 2.47 mmol) and cyanomethylenetributylphosphorane (1.19 g, 4.94 mmol) were dissolved in anhydrous toluene (20.0 mL). The mixture was reacted at 110° C. for 12 hours under nitrogen. The reaction solution was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to obtain compound 26-6.

[0768] Step 6: Synthesis of intermediates 26-7A and 26-7B

[0769] Compound 26-6 (1.10 g, 1.26 mmol) and methyl fluorosulfonyldifluoroacetate (919.60 mg, 4.79 mmol) were dissolved in anhydrous DMF (10.0 mL), and copper iodide (480 mg, 2.52 mmol) was added. The mixture was reacted at 110° C. for 2.5 hours. 50.0 mL of water was added at 25° C., and the mixture was extracted with ethyl acetate (150 mL). The organic phase was washed with saturated brine (150 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=5:1) to obtain compound 26-7. This was then separated by preparative SFC (chiral column: DAICEL CHIRALCEL OD (250 mm*30 mm, 10 μm); mobile phase: supercritical CO2-ethanol (0.1% aqueous ammonia); ethanol%: 40%-40%) to obtain compounds 26-7A and 26-7B. Chiral SFC analysis (chromatographic column: (S,S)Whelk-O1 50*4.6 mm, 3.5 μm; mobile phase: [supercritical CO2-ethanol (0.05% diethylamine)]; (ethanol (0.05% diethylamine))%: 50%-50%), compound 26-7A (Rt=1.791 min, ee value 99%, MS m / z=862.3 [M+H] + ). 1 H NMR(400MHz,CD3OD)δ ppm7.15(d,J=8.50Hz,4H),6.85(d,J=8.63Hz,4H),5.21-5.09(m,1H),4.70 (s,2H),4.21(s,6H),3.81(s,6H),3.57-2.97(m,5H),2.54(s,3H),1.96(br Compound 26-7B (Rt=2.166 min, ee value 99%, MS m / z=862.3[M+H] + ).

[0770] Step 7: Synthesis of intermediates 26-8A and 26-8B

[0771] Compound 26-7A (170 mg, 197 μmol) was dissolved in anhydrous dichloromethane (2.00 mL). Metachloroperbenzoic acid (44.0 mg, 217 μmol, 85% purity) was added. The mixture was reacted at room temperature for 2 hours. The mixture was extracted with dichloromethane (60.0 mL). The organic phase was washed with saturated brine (60.0 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by column chromatography (petroleum ether:ethyl acetate=2:1) ​​to give compound 26-8A. MS m / z=878.2[M+H] + .

[0772] Refer to step 7 and use 26-7B as starting material to give compound 26-8B. MS m / z=878.2[M+H] +

[0773] Step 8: Synthesis of intermediates 26-9A and 26-9B formate salts

[0774] Compound 26-8A (150 mg, 171 μmol) was dissolved in anhydrous toluene (2.00 mL). Sodium tert-butoxide (131 mg, 1.37 mmol), compound 8-2 (52.3 mg, 342 μmol) and molecular sieves (70.0 mg) were added. The mixture was reacted at 100° C. for 12 hours. 60.0 mL of saturated aqueous ammonium chloride solution was poured into the reaction solution. The mixture was extracted with ethyl acetate (50.0 mL). The organic phase was washed with saturated brine (60.0 mL), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure to give the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25mm*10μm; mobile phase: [water (0.225% formic acid)-acetonitrile]; acetonitrile: 40%-70%) and lyophilized to obtain compound 26-9A formate salt. MS m / z=967.3[M+H] + .

[0775] Refer to step 8 and use 26-8B as starting material to give compound 26-9B. MS m / z=967.3[M+H] +.

[0776] Step 9: Synthesis of intermediates 26-10A and 26-10B

[0777] Compound 26-9A formate (20.0 mg) was dissolved in anhydrous toluene (1.00 mL), and 1-propynyltri-n-butyltin (27.2 mg, 82.7 μmol) and dichlorobis[di-tert-butyl-(4-dimethylaminophenyl)phosphine]palladium(II) (4.39 mg, 6.20 μmol) were added. The atmosphere was replaced with nitrogen three times. The mixture was reacted under nitrogen at 110° C. for 12 hours. The mixture was concentrated under reduced pressure to remove toluene. 2.00 mL of ethyl acetate was added. The residue was purified by preparative thin layer chromatography to obtain compound 26-10A. MS m / z=971.3[M+H] + .

[0778] Refer to step 9 and use 26-9B formate salt as starting material to give compound 26-10B. MS m / z=971.3 [M+H] + .

[0779] Step 10: Synthesis of Compounds 26A and 26B Trifluoroacetate Salts

[0780] Compound 26-10A (40.0 mg, 41.2 μmol) was dissolved in dichloromethane (1.00 mL). Trifluoroacetic acid (307 mg, 2.69 mmol) was added. The mixture was reacted at 25° C. for 12 hours. 3.00 mL of saturated sodium carbonate solution was added to the reaction solution. The mixture was concentrated under reduced pressure to obtain the crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile: 15%-45%) and lyophilized to obtain 26A trifluoroacetate. MS m / z=631.3[M+H] + . 1H NMR(400MHz,CD3OD)δ ppm5.31(br d,J=7.88Hz,3H),4.84-4.83(m,2H),4.77-4.71(m,1H),4.58(br s,2H),4.43-4.31(m,2H),4.23-4.13(m,2H),3.98-3.64(m,4H),3.36(br s,1H),3.25(br d,J=2.88Hz,1H),3.09-2.91(m,2H),2.79(br d,J=16.01Hz,1H),2.44-2.34(m,1H),2.32-2.20(m,4H),2.18-2.05(m,5H),2.02-1.90(m,1H).

[0781] Compound 26-10B (46.0 mg, 47.4 μmol) was dissolved in dichloromethane (1.00 mL). Trifluoroacetic acid (307 mg, 2.69 mmol) was added. The mixture was reacted at 25° C. for 12 hours. The reaction solution was concentrated under reduced pressure to obtain a crude product. The crude product was purified by high performance liquid chromatography (column: Phenomenex luna C18 150*25 mm*10 μm; mobile phase: [water (0.075% trifluoroacetic acid)-acetonitrile]; acetonitrile: 15%-45%) and lyophilized to obtain 26B trifluoroacetate. MS m / z=631.3[M+H] + . 1 H NMR(400MHz,CD3OD)δ ppm5.34-5.16(m,3H),4.88(br s,2H),4.77-4.71(m,1H),4.56(s,2H),4.34(br d,J=14.26Hz,2H),4.20-4.10(m,2H),3.92(br d,J=14.63Hz,1H),3.86-3.63(m,3H),3.27-3.21(m,1H),3.08-2.89(m,3H),2.79(br d,J=15.76Hz,1H),2.36(br dd,J=11.88,6.38Hz,1H),2.30-2.10(m,7H),2.09(s,3H). Biological Test Data

[0782] Assay Example 1: Assay for p-ERK Inhibition in GP2D Cells

[0783] 1. Purpose

[0784] KRAS G12D Compounds that can effectively inhibit p-ERK in mutant GP2D cells were screened by HTRF method.

[0785] 2. Assay Process

[0786] 1) GP2D cells were seeded into a transparent 96-well cell culture plate. Each well contained 80 μL of cell suspension and 8000 cells. The cell plate was incubated overnight at 37° C. in a carbon dioxide incubator.

[0787] 2) 2 μL of compound was weighed and added to 78 μL of cell culture medium. After the mixture was mixed thoroughly, 20 μL of compound solution was weighed and added to the corresponding wells of the cell plate. The cell plate was placed back into the carbon dioxide incubator and incubated for an additional hour.

[0788] 3) After incubation was completed, the cell supernatant was discarded. 50 μL of 1x cell lysate was added to each well. The mixture was incubated at room temperature with shaking for 30 minutes.

[0789] 4) Phospho-ERK1 / 2 Eu cryptate antibody and phospho-ERK1 / 2 d2 antibody were diluted 20-fold with detection buffer.

[0790] 5) 16 μL of cell lysate supernatant was weighed and added to each well of a new 384 white microwell plate. Then 2 μL of a diluted solution of phospho-ERK1 / 2 Eu cryptate antibody and 2 μL of a diluted solution of phospho-ERK1 / 2 d2 antibody were added. The mixture was incubated at room temperature for at least 4 hours.

[0791] 6) After the incubation was completed, HTRF was read on a multi-label analyzer (excitation: 320 nm, emission: 615 nm, 665 nm).

[0792] 7) IC of the compound being assayed 50 was calculated.

[0793] 3. Assay Results

[0794] The results are shown in Table 5.

[0795] [Table 5]

[0796] Assay Conclusions: Compounds of the present disclosure inhibit KRAS G12D It has a significant inhibitory effect on p-ERK in mutant GP2D cells.

[0797] Assay Example 2: Assay for p-ERK Inhibition in AGS Cells

[0798] 1. Purpose

[0799] KRAS G12D Compounds that can effectively inhibit p-ERK in mutant AGS cells were screened by HTRF method.

[0800] 2. Assay Process

[0801] 1) AGS cells were seeded into a clear 96-well cell culture plate. Each well contained 80 μL of cell suspension and 10,000 cells. The cell plate was incubated overnight at 37° C. in a carbon dioxide incubator.

[0802] 2) After incubation was completed, the cell supernatant was discarded. 80 μL of medium containing 0.02% serum was added to each well. The cell plate was incubated at 37° C. overnight in a carbon dioxide incubator.

[0803] 3) 2 μL of compound was weighed and added to 78 μL of cell culture medium. After the mixture was mixed thoroughly, 20 μL of compound solution was weighed and added to the corresponding wells of the cell plate. The cell plate was placed back into the carbon dioxide incubator and incubated for an additional 3 hours.

[0804] 4) After incubation was completed, the cell supernatant was discarded. 50 μL of 1x cell lysate was added to each well. The mixture was incubated at room temperature with shaking for 30 minutes.

[0805] 5) Phospho-ERK1 / 2 Eu cryptate antibody and phospho-ERK1 / 2 d2 antibody were diluted 20-fold with detection buffer.

[0806] 6) 16 μL of cell lysate supernatant was weighed and added to each well of a new 384 white microwell plate. Then 2 μL of a diluted solution of phospho-ERK1 / 2 Eu cryptate antibody and 2 μL of a diluted solution of phospho-ERK1 / 2 d2 antibody were added. The mixture was incubated at room temperature for at least 4 hours.

[0807] 7) After the incubation was completed, HTRF was read on a multi-label analyzer (excitation: 320 nm, emission: 615 nm, 665 nm).

[0808] 8) IC of the compound being assayed 50 was calculated.

[0809] 3 Assay Results

[0810] The results are shown in Table 6.

[0811] [Table 6]

[0812] Assay Conclusions: Compounds of the present disclosure inhibit KRAS G12D It has a significant inhibitory effect on p-ERK in mutant AGS cells.

[0813] Assay Example 3: Assay of p-ERK Inhibition in AsPC-1 Cells

[0814] 1. Purpose

[0815] KRAS G12D Compounds that can effectively inhibit p-ERK in mutant AsPC-1 cells were screened by HTRF method.

[0816] 2. Assay materials:

[0817] ASPC-1 cells purchased from Procell; RPMI-1640 medium purchased from VivaCell; fetal bovine serum purchased from Biosera; and Advanced phospho-ERK1 / 2 (THR202 / TYR204) kit purchased from Cisbio.

[0818] [Table 7]

[0819] 3. Assay Method:

[0820] 6) ASPC-1 cells were seeded into a clear 96-well cell culture plate. Each well contained 80 μL of cell suspension and 8000 cells. The cell plate was incubated overnight at 37° C. in a carbon dioxide incubator.

[0821] 7) After incubation was completed, the cell supernatant was discarded. 80 μL of RPMI-1640 blank medium was added to each well and starved overnight.

[0822] 8) The compounds to be assayed were diluted with 100% DMSO to 2 mM as the first concentration, then serially diluted 5-fold using a pipette to eight concentrations (i.e., 2 mM to 25.6 nM). 2 μL of compound was weighed and added to 78 μL of cell starvation medium. After the mixture was mixed thoroughly, 20 μL of compound solution was weighed and added to the corresponding wells of the cell plate. The cell plate was placed back into the carbon dioxide incubator and incubated for an additional hour. At this time, the compound concentrations were 10 μM to 0.128 nM, and the DMSO concentration was 0.5%.

[0823] 9) After incubation was completed, the cell supernatant was discarded. 50 μL of cell lysate was added to each well. The mixture was incubated at room temperature with shaking for 30 minutes.

[0824] 10) Phospho-ERK1 / 2 Eu cryptate antibody and phospho-ERK1 / 2 d2 antibody were diluted 20-fold with detection buffer.

[0825] 11) 16 μL of cell lysate supernatant was weighed and added to each well of a new 384 white microwell plate. Then 2 μL of a diluted solution of phospho-ERK1 / 2 Eu cryptate antibody and 2 μL of a diluted solution of phospho-ERK1 / 2 d2 antibody were added. The mixture was incubated at room temperature for 4 hours.

[0826] 12) After incubation was completed, HTRF was read on a multi-label analyzer (excitation: 320 nm, emission: 615 nm, 665 nm).

[0827] 4. Data Analysis:

[0828] Raw data were converted to percentage inhibition using the formula: (sample-min) / (max-min)*100%. IC was calculated by curve fitting with four parameters (GraphPad Prism, “log(inhibitor) vs. response--Variable slope” mode). 50Values ​​can be obtained. Table 1 shows the inhibitory effect of the compounds of the present disclosure on p-ERK.

[0829] Maximum well: Positive control well reading was the 1x lysate reading.

[0830] Min Well: The reading of the negative control well was the reading of the cell lysate in the 0.5% DMSO cell well.

[0831] 5. Assay Results

[0832] The results are shown in Table 8.

[0833] [Table 8]

[0834] Assay Conclusions: Compounds of the present disclosure inhibit KRAS G12D It has a significant inhibitory effect in mutant AsPC-1 cells.

[0835] Assay Example 4: Assay of p-ERK inhibition in AsPC-1 cells

[0836] 1. Purpose

[0837] KRAS G12D Compounds that could effectively inhibit p-ERK in mutant AsPC-1 cells were screened by in-cell Western analysis.

[0838] 2. Assay materials:

[0839] ASPC-1 cells purchased from ATCC; RPMI-1640 medium purchased from ATCC; fetal bovine serum purchased from Ausgenex; phospho-p44 / 42 MAPK (Erk1 / 2) (Thr202 / Tyr204) (D13.14.4E) XP(R) rabbit mAb purchased from CST; GAPDH (D4C6R) mouse mAb purchased from CST; IRDye 680RD goat anti-mouse IgG (H+L) purchased from LI-COR, and IRDye 800CW goat anti-rabbit IgG (H+L) purchased from LI-COR.

[0840] 3. Assay Method:

[0841] 13) ASPC-1 cells were seeded into black bottom clear 384-well cell culture plates. Each well contained 40 μL of cell suspension and 6000 cells. The cell plates were incubated overnight at 37°C in a carbon dioxide incubator.

[0842] 14) The compounds to be assayed were diluted with 100% DMSO to a first concentration of 10 mM, then serially diluted 3-fold with a pipette to nine concentrations (i.e., 10 mM to 1.52 μM). 40 nL of compound was transferred to the cell plate with the ECHO device and centrifuged at low speed. The cell plate was placed back into the carbon dioxide incubator and incubated for an additional hour. At this time, the compound concentrations were 10 μM to 1.52 nM, and the DMSO concentration was 0.1%.

[0843] 15) After incubation was completed, 40 μL of 8% paraformaldehyde fixative was added to each well and allowed to stand at room temperature for 20 minutes.

[0844] 16) The wells were washed twice with PBS buffer. 40 μL of pre-chilled methanol solution was added to each well. The wells were washed twice with PBS buffer. 20 μL of blocking solution was added to each well and left to stand at room temperature for 60 minutes.

[0845] 17) The blocking solution was removed and 20 μL of the primary antibody mixture (1:1000 dilution of phospho-p44 / 42 MAPK (Erk1 / 2) (Thr202 / Tyr204) (D13.14.4E) XP(R) rabbit mAb, 1:2000 dilution of GAPDH (D4C6R) mouse mAb) was added to each well and incubated overnight at 4°C.

[0846] 18) The primary antibody mixture was removed and the cells were washed three times with PBS buffer. 20μL of the secondary antibody mixture (IRDye 800CW goat anti-rabbit IgG (H+L) diluted 1:2000 and IRDye 680RD goat anti-mouse IgG (H+L) diluted 1:2000) was added to each well and incubated at room temperature in the dark for 60 minutes.

[0847] 19) The primary antibody mixture was removed and the cells were washed three times with PBS buffer. The plates were centrifuged upside down at 1000 rpm for 1 minute and then scanned using an Odyssey CLx instrument.

[0848] 4. Data Analysis:

[0849] Relative expression levels were calculated using the formula: (total fluorescence value of 800 channel / total fluorescence value of 700 channel), and raw data were converted to percentage inhibition using the formula: (max-sample) / (max-min)*100%. IC was calculated by four-parameter curve fitting (log(inhibitor) vs. response--Variable slope mode in Excel plugin XLfit). 50 Got the value.

[0850] Maximum well: The reading of the positive control well was the highest concentration of positive drug.

[0851] Min Well: The reading of the negative control well was the reading in the 0.1% DMSO cell well.

[0852] 5. Assay Results

[0853] The results are shown in Table 9.

[0854] [Table 9]

[0855] Assay Conclusions: Compounds of the present disclosure inhibit KRAS G12D It has a significant inhibitory effect on p-ERK in mutant AsPC-1 cells.

[0856] Assay Example 5: Anti-cell proliferation effect of compounds in tumor cell line AsPC-1

[0857] Test Purpose

[0858] In this assay, the effect of compounds on inhibiting cell proliferation was assessed using the KRAS G12D The compounds were tested by detecting their effects on in vitro cellular activity in the mutant tumor cell line AsPC-1.

[0859] Assay materials

[0860] Cell line: AsPC-1; Tumor type: Pancreatic cancer; Growth characteristics: Adherent growth; Culture method: RPMI 1640 + 10% FBS

[0861] Ultra Low Cluster 96-well plate (Corning-7007)

[0862] Greiner CELLSTAR 96-well plate (#655090)

[0863] Promega CellTiter-Glo 3D Luminescent Cell Viability Assay Kit (Promega-G9683)

[0864] 2104-10 EnVision plate reader (PerkinElmer)

[0865] RPMI 1640, DMEM, PBS (phosphate buffered saline), FBS (fetal bovine serum), antibiotic-antimycotic, L-glutamine, DMSO (dimethyl sulfoxide)

[0866] Assay Methods and Procedures

[0867] cell culture

[0868] Tumor cell lines were cultured in a 5% CO2 incubator at 37°C according to the culture conditions indicated in the culture method. Cells were passaged periodically, and cells in the logarithmic growth phase were used for plating.

[0869] Cell plating

[0870] Cells were stained with trypan blue and viable cells were counted.

[0871] The cell concentration was adjusted to an appropriate concentration.

[0872] Cell line: AsPC-1; density (per well): 7000 cells.

[0873] 135 μL of cell suspension was added to each well of the ULA culture plate. Blank control wells received the same volume of medium without cells.

[0874] After plating, the ULA culture plate was immediately centrifuged at 1000 rpm for 10 min at room temperature. NOTE: After centrifugation is complete, subsequent operations must be performed carefully to avoid unnecessary shocks.

[0875] The culture plates were incubated overnight in a 37° C., 5% CO 2 incubator with 100% relative humidity.

[0876] Formulation of 10x compound standard solutions and cell treatment with compounds (Day 1)

[0877] 10x compound standard solutions (10x standard solutions in DMSO) were formulated. 15 μL of 10x compound standard solutions were added to the ULA culture plates, and 15 μL of DMSO cell media mixture was added to the vehicle control and blank control, respectively.

[0878] The 96-well cell plate was placed back into the incubator and incubated for 120 hours.

[0879] The cells were observed for sphere formation every day until the end of the assay.

[0880] CellTiter-Glo Luminescent Cell Viability Detection (Day 5)

[0881] The following steps were performed according to the instructions of the Promega CellTiter-Glo 3D Luminescent Cell Viability Assay Kit (Promega #G9683).

[0882] 150 μL of CellTiter-Glo 3D Reagent (equal to the volume of cell culture medium in each well) was added to each well. The cell plates were wrapped in aluminum foil to protect from light.

[0883] The culture plate was shaken on an orbital shaker for 5 minutes.

[0884] To ensure that the cell spheroids were adequately detached before proceeding to the next step, the mixture in the well was thoroughly mixed by carefully pipetting up and down 10 times with a pipette.

[0885] The solution in the ULA plate was then transferred to a black bottom culture plate (#655090) and left at room temperature for 25 minutes to allow the luminescence signal to stabilize.

[0886] Luminescent signals were detected with a 2104 EnVision plate reader.

[0887] Data analysis

[0888] The IR of the assay compounds was calculated using the formula: Inhibition percentage (IR) (%) = (1-(RLU of compound-RLU of blank control) / (RLU of vehicle control-RLU of blank control))*100%. The inhibition percentages of different concentrations of compounds were calculated in Excel, and then inhibition curves were generated using GraphPad Prism software to record the minimum inhibition percentage, maximum inhibition percentage and IC 50 The relevant parameters were calculated, including:

[0889] Assay Results

[0890] The results are shown in Table 10.

[0891] [Table 10]

[0892] Assay Conclusions: Compounds of the present disclosure inhibit KRAS G12D Shows a significant anti-proliferative effect on mutant AsPC-1 cells.

[0893] Assay Example 6: Antiproliferative effects of compounds in the tumor cell line AsPC-1

[0894] 1. Purpose

[0895] KRAS G12D The anti-cell proliferation effect of the compounds in the mutant tumor cell line AsPC-1 was verified by 3D-CTG method.

[0896] 2. Assay materials:

[0897] ASPC-1 cells purchased from ATCC; RPMI-1640 medium purchased from ATCC; fetal bovine serum purchased from Augenex; CellTiter-Glo® 3D Assay Kit (3D-CTG) purchased from Promega; and CellCarrier-96 spheroids ULA / CS purchased from PE.

[0898] 3. Assay Method:

[0899] 20) ASPC-1 cells were seeded into a clear 96-well cell culture plate. Each well contained 195 μL of cell suspension and 2000 cells.

[0900] 21) The compounds to be assayed were diluted to 10 mM as the first concentration using 100% DMSO, then serially diluted 5-fold using a pipette to eight concentrations (i.e., 10 mM to 0.13 μM). 2 μL of serially diluted compound was weighed and added to 48 μL of cell medium for the second dilution. After the mixture was mixed thoroughly, 5 μL of the second diluted compound was weighed and added to the corresponding 195 μL well of the cell plate. The cell plate was placed back into the carbon dioxide incubator and incubated for 7 days. At this time, the concentrations of the compounds were 10 μM to 0.128 nM, and the concentration of DMSO was 0.1%.

[0901] 22) After the incubation was completed, 100 μL of cell supernatant was discarded. 60 μL of 3D-CTG was added to each well. The mixture was incubated at room temperature for 20 minutes with shaking at 200 rpm, and the cell plate was incubated in an incubator at room temperature for 1 hour.

[0902] 23) 100 μL of the supernatant from the plate was transferred to a 96-well black bottom clear plate and the luminescence was read on a BMG.

[0903] 4. Data Analysis:

[0904] Raw data were converted to percent inhibition using the formula: % inhibition = (mean_H-sample) / (mean_H-mean_L). IC was calculated by curve fitting with four parameters (GraphPad Prism's "log(inhibitor) vs. response--Variable slope" mode). 50 The value can be obtained.

[0905] H well: DMSO well reading

[0906] L well: medium reading

[0907] 5. Assay Results

[0908] The results are shown in Table 11.

[0909] [Table 11]

[0910] Assay Conclusions: Compounds of the present disclosure inhibit KRAS G12D Shows a significant anti-proliferative effect on mutant AsPC-1 cells.

[0911] Assay Example 7: Oral and intravenous injection assays in CD-1 mice Pharmacokinetic testing of compounds

[0912] Assay Purpose

[0913] To assay the in vivo pharmacokinetics of orally and intravenously injected compounds in CD-1 mice.

[0914] Assay process

[0915] The assay compounds were mixed with 5% DMSO + 95% (10% HP-β-CD) aqueous solution. The mixture was vortexed and sonicated to prepare a clear solution of 0.5 mg / mL (intravenous) or a clear solution of 3 mg / mL (oral). For use, the clear solution was filtered through a microporous membrane. Male CD-1 mice aged 7 to 10 weeks were selected and administered the candidate compound solution intravenously and the candidate compound solution orally. Whole blood was collected at a certain period and prepared to obtain plasma. Drug concentrations were analyzed by LC-MS / MS method, and pharmacokinetic parameters were calculated with Phoenix WinNonlin software (Pharsight, USA).

[0916] Assay Results

[0917] [Table 12]

[0918] [Table 13]

[0919] Assay Results

[0920] The compounds of the present disclosure have long half-lives, high exposure, good oral bioavailability and good pharmacokinetic properties in mice. Assay Example 8: In vivo Pharmacodynamics Assay

[0921] Assay Method

[0922] A Balb / c nude mouse model of subcutaneous xenograft tumors of human colon cancer GP2D cells was established. 0.2 mL (2 × 10 6 ) was inoculated subcutaneously into the right dorsal region of each mouse. The average tumor volume was 140 mm 3 When the immunization dose reached 100 mg / kg, the mice were divided into groups of 6 mice each and administered. On the day of the assay, the animals were administered the corresponding drugs according to their corresponding groups. The first group G1 was set as a negative control group, which was given only 5% DMSO + 95% (10% HP-β-CD) by oral gavage. The second group G2 to the fourth group G4 were given compound 8 hydrochloride. The dosage and administration protocol are shown in Table 14.

[0923] [Table 14]

[0924] NOTE: PO means oral administration, QD means once daily, and BID means once daily.

[0925] During the assay, the body weight and tumor size of the animals were measured twice a week, while the clinical symptoms of the animals were observed and recorded daily. Each administration was based on the most recent body weight of the animals.

[0926] The length (a) and width (b) of the tumor were measured using a digital caliper. The tumor volume (TV) was calculated using the formula TV = a × b 2 / 2.

[0927] Assay Results:

[0928] Compound 8 hydrochloride has a significant inhibitory effect on xenograft tumors of human colon cancer GP2D in mice. After 28 days of administration, the second group G2 (50 mg / kg, PO, QD) has a tumor volume inhibition rate TGI (%) of 47.0% on the 28th day, the third group G3 (100 mg / kg, PO, QD) and the fourth group G4 (200 mg / kg, PO, QD) have tumor volume inhibition rates TGI (%) of 75.9% and 94.7% on the 28th day, respectively. The detailed results are shown in Table 15.

[0929] [Table 15]

[0930] Note: N / A means no detection.

[0931] Assay conclusion: Regarding in vivo efficacy, the compounds of the present invention exhibit good tumor inhibitory effect in GP2D cell line, with obvious dose-dependency.

Claims

1. Formula (VI) 【Chemistry 1】 or a pharma- ceutically acceptable salt thereof, During the ceremony, 【Chemistry 2】 is selected from a single bond and a double bond; T １ But, CR ａ and N; T ２ is selected from CH and N; L １ is selected from O and S; R １ However, phenyl, naphthyl, 【Chemistry 3】 The above phenyl, naphthyl, 【Chemistry 4】 each independently represents 1, 2, 3, 4 or 5 R ｂ Optionally replaced by R ２ is H, R ３ But, H, F, CN, CH ３ and O.C.H. ３ and the CH ３ and O.C.H. ３ is optionally substituted with 1, 2 or 3 halogens; Alternatively, R ２ and R ３ together with the atom to which they are attached form a phenyl group or a 5- to 6-membered heteroaryl group, said phenyl group or 5- to 6-membered heteroaryl group being optionally substituted with one, two or three halogens; R ４ is not present or is H, CH ３ and -CH=CH ２ and the CH ３ and -CH=CH ２ is optionally substituted with 1, 2 or 3 halogens; Alternatively, R ３ and R ４ along with the atoms to which they are attached. 【Chemistry 5】 Form the above 【Chemistry 6】 But one or two R ｃ Optionally replaced by Alternatively, R ３ and R ４ together with the atom to which they are attached form a cyclopropyl group optionally substituted with 1, 2 or 3 halogens; R ａ is selected from H and CN; Each R ｂ is H, F, Cl, Br, I, OH, NH ２ , C.N., C. １－３ Alkyl, C １－３ Alkoxy, C ２－４ Alkynyl, C ２－４ Alkynyl-cyclopropyl, C ２－３ Alkenyl, -C(=O)C １－３ Alkyl and C ３－５ cycloalkyl; １－３ Alkyl, C １－３ Alkoxy, C ２－４ Alkynyl, C ２－４ Alkynyl-cyclopropyl, C ２－３ Alkenyl, -C(=O)C １－３ Alkyl and C ３－５ cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 halogen; Each R ｃ F, Cl, Br, I, CN, CH ３ and O.C.H. ３ are independently selected from A compound or a pharma- ceutically acceptable salt thereof, wherein m is selected from 0 and 1.

2. Formula (P-1) 【Chemistry 7】 or a pharma- ceutically acceptable salt thereof, During the ceremony, 【Chemistry 8】 is selected from a single bond and a double bond; L １ is selected from O and S; R １ phenyl, naphthyl, 5- to 10-membered heteroaryl, 【Chemistry 9】 phenyl, naphthyl, 5- to 10-membered heteroaryl; 【Chemistry 10】 each independently represents 1, 2, 3, 4 or 5 R ｂ and optionally substituted with R ５ but, 【Chemistry 11】 Selected from the above 【Chemistry 12】 each independently represents 1, 2, 3, 4 or 5 R ｄ Optionally replaced by Alternatively, R １ is a 5- to 10-membered heteroaryl, and the 5- to 10-membered heteroaryl is selected from 1, 2, 3, 4, or 5 R ｂ and optionally substituted with R ５ but, 【Chemistry 13】 And the above 【Chemistry 14】 1, 2, 3, 4 or 5 R ｄ Optionally replaced by Each R ｂ is H, F, Cl, Br, I, OH, NH ２ , C.N., C. １－３ Alkyl, C １－３ Alkoxy, C ２－４ Alkynyl, C ２－４ Alkynyl-cyclopropyl, C ２－３ Alkenyl, -C(=O)C １－３ Alkyl and C ３－５ cycloalkyl; １－３ Alkyl, C １－３ Alkoxy, C ２－４ Alkynyl, C ２－４ Alkynyl-cyclopropyl, C ２－３ Alkenyl, -C(=O)C １－３ Alkyl and C ３－５ cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 R; Each R ｄ F, Cl, Br, I, CN, CH ３ and O.C.H. ３ are independently selected from or a pharma- ceutically acceptable salt thereof, wherein each R is independently selected from halogen and D.

3. Formula (P-2) 【Chemistry 15】 or a pharma- ceutically acceptable salt thereof, During the ceremony, 【Chemistry 16】 is selected from a single bond and a double bond; L １ is selected from O and S; Each R ｂ is F, Cl, Br, I, OH, NH ２ , C.N., C. １－３ Alkyl, C １－３ Alkoxy, C ２－４ Alkynyl, C ２－４ Alkynyl-cyclopropyl, C ２－３ Alkenyl, -C(=O)C １－３ Alkyl and C ３－５ cycloalkyl; １－３ Alkyl, C １－３ Alkoxy, C ２－４ Alkynyl, C ２－４ Alkynyl-cyclopropyl, C ２－３ Alkenyl, -C(=O)C １－３ Alkyl and C ３－５ cycloalkyl is optionally substituted with 1, 2, 3, 4 or 5 R; each R is independently selected from halogen and D; v is selected from 1, 2, 3, 4 and 5; However, at least one R ｂ is substituted with 1, 2, 3, 4 or 5 D, or a pharma- ceutically acceptable salt thereof.

4. Each R ｂ is H, F, Cl, Br, I, OH, NH ２ , CN, CH ３ , CH ２ CH ３ , OCH ３ , OCH ２ CH ３ , -CH=CH ２ , 【Chemistry 17】 and cyclopropyl, ３ , C.H. ２ CH ３ , O.C.H. ３ , O.C.H. ２ CH ３ , -CH=CH ２ , 【Chemistry 18】 and cyclopropyl is optionally substituted with 1, 2, 3, 4 or 5 halogen; alternatively, each R ｂ is H, F, Cl, Br, I, OH, NH ２ , C.N., C.H. ３ , C.H. ２ F, C.F. ２ H, C.F. ３ , C.H. ２ CH ３ , C.F. ２ CF ３ , -CH=CH ２ , 【Chemistry 19】 2. The compound of claim 1, or a pharma- ceutically acceptable salt thereof, wherein: R is independently selected from cyclopropyl, cyclopropyl, and cyclopropyl.

5. Each R ｂ is H, F, Cl, Br, I, OH, NH ２ , CN, CH ３ , CH ２ CH ３ , OCH ３ , OCH ２ CH ３ , -CH=CH ２ , 【Chemistry 20】 and cyclopropyl, ３ , C.H. ２ CH ３ , O.C.H. ３ , O.C.H. ２ CH ３ , -CH=CH ２ , 【Chemistry 21】 and cyclopropyl is optionally substituted with 1, 2, 3, 4 or 5 R; alternatively, each R ｂ is H, F, Cl, Br, I, OH, NH ２ , C.N., C.H. ３ , C.D. ３ , C.H. ２ F, C.F. ２ H, C.F. ３ , C.H. ２ CH ３ , C.F. ２ CF ３ , -CH=CH ２ , 【Chemical 22】 4. The compound of claim 2 or 3, or a pharma- ceutically acceptable salt thereof, wherein: R is independently selected from cyclopropyl, cyclopropyl, and cyclopropyl.

6. R １ but, 【Chemistry 23】 2. The compound of claim 1, selected from:

7. R １ but, 【Chemistry 24】 3. The compound of claim 2, selected from:

8. R ３ But, H, F, CN, CH ３ and O.C.H. ３ 2. The compound of claim 1, selected from:

9. R ２ and R ３ or a pharma- ceutically acceptable salt thereof, wherein, together with the atom to which they are attached, form a phenyl group, a furyl group, and a pyridyl group, said phenyl group, furyl group, and pyridyl group being optionally substituted with one F.

10. R ４ is not present or is H, CH ２ F and -CH=CH ２ 2. The compound of claim 1, selected from:

11. R ３ and R ４ along with the atoms to which they are attached. 【Chemistry 25】 2. The compound of claim 1, which forms:

12. R ３ and R ４ along with the atoms to which they are attached. 【Chemistry 26】 2. The compound of claim 1, which forms:

13. structural part 【Chemical 27】 but, 【Chemistry 28】 2. The compound of claim 1, selected from:

14. structural part 【Chemical 29】 but, 【Chemistry 30】 2. The compound of claim 1, selected from:

15. structural part 【Chemistry 31】 but, 【Chemistry 32】 4. The compound of claim 1, 2 or 3, which is: or a pharma- ceutically acceptable salt thereof.

16. 【Chemical 33】 During the ceremony, R ｂ and L １ each of which is as defined in claim 1; 2. The compound of claim 1, wherein n is selected from 0, 1, 2, 3, 4 and 5, or a pharma- ceutically acceptable salt thereof.

17. 【Catalogue 34】 【Chemistry 35】 【Chemical 36】 【Chemical 37】 【Chemical Formula 38】 【Chemical 39】 or a pharma- ceutically acceptable salt thereof.

18. 【Catalogue 40】 【Chemistry 41】 【Chemistry 42】 【Chemistry 43】 【Chemistry 44】 【Chemistry 45】 【Chemistry 46】 【Chemistry 47】 【Chemistry 48】 【Chemistry 49】 【Chemistry 50】 【Chemistry 51】 【Chemistry 52】 【Chemistry 53】 【Chemical 54】 【Chemistry 55】 【Chemistry 56】 【Chemistry 57】 18. The compound of claim 17, selected from: or a pharma- ceutically acceptable salt thereof.

19. KRAS Ｇ１２Ｄ 20. Use of a compound according to any one of claims 1 to 18, or a pharma- ceutically acceptable salt thereof, in the manufacture of a medicament for treating a disease associated with a mutation.

20. 20. Use of a compound according to any one of claims 1 to 19, or a pharma- ceutically acceptable salt thereof, in the manufacture of a medicament for treating a tumor-related disease.

Images