Cell Processing Device

Abstract

The present invention relates to the technical field of cell processing, and more particularly to a cell processing device. The weighing device of this cell processing device is equipped with at least one weighing unit. The weighing unit can simultaneously measure and detect one or more samples hung from hooks, improving weighing efficiency. Each sample hung from each hook is weighed by a corresponding weighing sensor. This prevents the measurement results from interfering with each other. Each weighing unit of the present invention is detachable, facilitating inspection, repair, and maintenance. Furthermore, the present invention uses a centrifuge module arranged for the centrifuge cup to mount and rotate the centrifuge cup, which makes the centrifuge cup easy to mount and secure, preventing the centrifuge cup from falling off and ensuring the safety of the cell processing process. The present invention has a simple structure, a small device volume, and is easy to inspect and repair.

Description

[Technical Field]

[0001] The present invention claims priority from a Chinese patent application filed with the China Patent Office on July 22, 2022, bearing application number 202210870017.7 and titled "Cell Treatment Device," the entire contents of which are incorporated herein by reference. The present invention belongs to the technical field of cell processing, and in particular to a cell processing device. [Background technology]

[0002] Currently, cell processing, such as cell separation and mixing, is typically performed using a cell processing device. Conventional cell processing devices typically have a single function and are large in volume. The inventors discovered that in the process of performing cell processing using a cell processing device, the weighing device can only weigh one set of samples to be processed at a time, resulting in low weighing efficiency. Furthermore, the large volume of the weighing device makes troubleshooting complicated in the event of a breakdown, making inspection and repair difficult. Summary of the Invention [Problem to be solved by the invention]

[0003] The present invention provides a cell treatment device that overcomes technical problems such as the single function and low metering efficiency of the prior art cell treatment devices. [Means for solving the problem]

[0004] In view of the above technical problems, an embodiment of the present invention provides a cell treatment device, which comprises: a weighing device including a support unit and at least one set of weighing units, the support unit including a bracket for supporting the weighing units, and each set of the weighing units including a mounting holder attached to the bracket, a load cell attached to the mounting holder, and a hook detachably attached to the load cell for suspending a sample; a centrifugal device including a centrifuge pot having a centrifuge chamber, a centrifuge cup installed in the centrifuge chamber, and a centrifuge module connected to the centrifuge pot for rotating the centrifuge cup; a liquid path module including a pipeline unit for transporting the liquid in the sample into the centrifuge cup for cell processing, and a liquid path panel for mounting the pipeline unit and controlling the flow of the liquid in the pipeline unit; and a housing to which the centrifuge pot, the liquid passage panel, and the bracket are all attached.

[0005] In the cell processing device of the present invention, at least one weighing unit is installed in the weighing device. The weighing unit can simultaneously weigh and detect one or more samples hung from hooks, improving weighing efficiency. Furthermore, the weighing unit is equipped with hooks for hanging samples, which facilitates sample removal and prevents the flow of liquid within the hanging samples from being interrupted. Furthermore, when multiple samples are weighed simultaneously, each hanging sample on a hook is weighed by its corresponding load cell, ensuring that the weighing results for each sample are independent and do not interfere with each other. Each weighing unit of the present invention is attached to a bracket by a mounting holder. This allows for the attachment and detachment of each weighing unit, facilitating inspection, repair, and maintenance. Furthermore, in the present invention, the centrifuge cup is attached to and rotated by a centrifuge module arranged for the centrifuge cup, making the attachment of the centrifuge cup more convenient and secure, preventing the centrifuge cup from falling off, and ensuring the safety of the cell processing process. The cell treatment device of the present invention has a simple structure, allows the device volume to be small, and each part is independently and detachably attached to the housing, which simplifies the troubleshooting process in the event of a malfunction and makes inspection and repair easier.

[0006] The details of one or more embodiments of the invention are set forth in the drawings and description below. Other features and advantages of the invention will become apparent from the description, drawings, and claims. [Brief explanation of the drawings]

[0007] The present invention will be further described below with reference to the drawings and examples. [Figure 1] 1 is a diagram showing the configuration of a cell treatment device provided in one embodiment of the present invention. [Figure 2] FIG. 2 is an exploded view showing the configuration of a measuring device of a cell treatment device provided in one embodiment of the present invention. [Figure 3] FIG. 2 is an exploded view showing the configuration of a measuring unit of a cell treatment device provided in one embodiment of the present invention. [Figure 4] 10A and 10B are diagrams showing the configuration of a hook of a cell treatment device provided in one embodiment of the present invention. [Figure 5] 10A and 10B are diagrams showing the configuration of a hook of a cell treatment device provided in another embodiment of the present invention. [Figure 6] 1 is an assembly diagram showing the configuration of a centrifugal module of a cell treatment device provided in one embodiment of the present invention. [Figure 7] FIG. 2 is an exploded view showing the configuration of a centrifugal module of a cell treatment device provided in one embodiment of the present invention. [Figure 8] FIG. 2 is an exploded view showing the configuration of a centrifugal module and a temperature control module of a cell treatment device provided in one embodiment of the present invention. [Figure 9] 1 is an assembly diagram showing the configuration of a centrifugal module and a temperature control module of a cell treatment device provided in one embodiment of the present invention. [Figure 10] FIG. 2 is a diagram showing the configuration of a liquid channel module of a cell treatment device provided in one embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0008] In order to clarify the technical problems, technical solutions and beneficial effects that the present invention aims to solve, the present invention will be described in more detail below with reference to the accompanying drawings and examples. It should be understood that the specific examples described herein are for interpreting the present invention, rather than for limiting the present invention.

[0009] It should be noted that terms indicating directions or positional relationships, such as "upper," "lower," "left," "right," "front," "rear," and "middle," are based on directions or positional relationships shown in the drawings and are merely intended to facilitate and simplify the description of the present invention, and do not indicate or imply that the devices or elements referred to must have a specific direction, configuration, or operation in a specific direction, and cannot be considered to limit the present invention.

[0010] As shown in FIGS. 1 and 2, one embodiment of the present invention provides a cell processing device, which includes a metering device 1, a centrifugal device 2, a fluid path module 3 and a housing 5. The weighing device 1 includes a support unit 11 and at least one set of weighing units 12. The support unit 11 includes a bracket 111 for supporting the weighing units 12. Each set of the weighing units 12 includes a mounting holder 121 attached to the bracket 111, a load cell 122 attached to the mounting holder 121, and a hook 123 detachably attached to the load cell 122 for suspending a sample 4 (e.g., a liquid bag for placing a liquid). The load cell 122 may be a pressure-sensing member for determining weight based on a pressure signal. Both the bracket 111 and the mounting holder 121 may be made of sheet metal. This makes the bracket 111 stronger and achieves better load-bearing capacity, while the mounting holder 121 made of sheet metal, which is less likely to deform, better protects the load cell 122 and other components. The shape and size of the bracket 111 can be set as needed, allowing the weighing units 12 to be stably attached to the bracket 111 while minimizing the overall volume of the weighing device 1. In the above-described embodiment of the present invention, one or more weighing units 12 of the same configuration are installed within the weighing device 1 (the number of weighing units 12 can be set as needed). Each weighing unit 12 is attached to the bracket 111 by a mounting holder 121, which allows for easy attachment and detachment of one weighing unit 12, and damage to one weighing unit 12 does not affect the use of the other weighing units 12. Furthermore, since each weighing unit 12 has the same configuration, inspection, repair, and maintenance are simplified. The hook 123 is designed to be detachably connected to the load cell 122, which facilitates attachment and detachment of the hook 123 and improves troubleshooting efficiency. In one embodiment, a threaded hole (not shown) is provided in the load cell 122, a connecting screw 1231 is provided in the hook 123, and the hook 123 is screwed into the threaded hole by the connecting screw 1231. That is, in this embodiment, the hook 123 and the load cell 122 are detachably connected by the screw connection between the connecting screw 1231 and the threaded hole.

[0011] 2 and 3, the mounting holder 121 has a receiving groove 1211 that engages with the load cell 122. A fixing hole 1212 is formed in the inner wall of the receiving groove 1211. The load cell 122 is fixedly mounted in the receiving groove 1211 by a screw that passes through the fixing hole 1212. That is, the mounting holder 121 may be a bent plate that is bent to form the receiving groove 1211. Mounting the load cell 122 in the receiving groove 1211 protects the load cell 122 from damage. Because the lower end of the load cell 122 needs to be connected to the hook 123, the fixing hole 1212 in this embodiment is located on the inner wall of the upper portion of the receiving groove 1211. This fixes the upper portion of the load cell 122 to the mounting holder 121, preventing it from falling off. Optionally, the mounting holder 121 may include a flange 1213 that is located on the edge of the receiving groove 1211. The mounting holder 121 is attached to the bracket 111 by the flange 1213. Specifically, the mounting holder 121 is fixed to the mounting plate 1111 by the flange 1213. The connection between the two may be a detachable connection such as a screw connection or a locking connection. This makes it easy to detach the mounting holder 121 and the entire weighing unit 12 from the mounting plate 1111.

[0012] The centrifuge device 2 includes a centrifuge pot 21 having a centrifuge chamber, a centrifuge cup (not shown) installed in the centrifuge chamber, and a centrifuge module 100 connected to the centrifuge pot 21 for rotating the centrifuge cup. The centrifuge module 100 of the centrifuge device 2 rotates the centrifuge cup located in the centrifuge chamber of the centrifuge pot 21 to perform cell processing on the liquid poured into the centrifuge cup. In this embodiment, the centrifuge cup is attached to and rotated by the centrifuge module 100 arranged for the centrifuge cup, making the attachment of the centrifuge cup more convenient and secure, preventing the centrifuge cup from falling off, and ensuring the safety of the cell processing process.

[0013] The fluid path module 3 includes a conduit unit 31 for transporting the liquid in the sample 4 into the centrifuge cup for cell processing, and a fluid path panel 32 for mounting the conduit unit 31 and controlling the flow of liquid in the conduit unit 31. That is, in the above-mentioned fluid path module 3, as shown in Fig. 10, the conduit unit 31 is mounted on the fluid path panel 32, and by controlling the conduit unit 31 by operating the fluid path panel 32, the liquid in the sample 4 can be smoothly flowed into the centrifuge cup by the conduit unit 31, thereby performing cell processing.

[0014] The centrifuge pot 21, the liquid passage panel 32, and the bracket 111 are all attached to the housing 5. The shape of the housing 5 is not limited to that shown in FIG. 1 and can be set as needed. The housing 5 includes a support frame, a bottom plate attached to the bottom of the support frame, side plates attached to the periphery of the support frame, and a top plate attached to the top of the support frame. The bottom plate, top plate, and side plates can be assembled by screw connections, locking connections, welding, or other methods. The bottom plate, top plate, and side plates can all be installed so that they can be detachably connected to the support frame. This makes it easy to install and maintain components inside the housing 5 (for example, inserting pipes or replacing the centrifuge cup) from the removed locations.

[0015] The weighing device 1 of the cell processing device of the present invention is equipped with at least one weighing unit 12. The weighing unit 12 can simultaneously measure and detect one or more samples 4 suspended from hooks 123, improving weighing efficiency. Furthermore, the weighing unit 12 is equipped with hooks 123 for suspending the samples 4, which facilitates suspending the samples 4 and prevents the flow of liquid within the suspended samples 4 from being impeded. Furthermore, when multiple samples 4 are simultaneously weighed, each sample 4 suspended from each hook 123 is weighed by its corresponding load cell 122, ensuring that the measurement results for each sample 4 are independent and do not interfere with each other. Furthermore, each weighing unit 12 of the present invention is attached to a bracket 111 by a mounting holder 121. Therefore, a single weighing unit 12 can be attached and detached, facilitating inspection, repair, and maintenance. Furthermore, in the present invention, the centrifuge cup is attached to and rotated by the centrifuge module 100 arranged for the centrifuge cup, making the attachment of the centrifuge cup more convenient and secure, preventing the centrifuge cup from falling off and ensuring the safety of the cell treatment process. The cell treatment device of the present invention has a simple structure, can be made small in volume, and each part is independently and detachably attached to the housing 5, which simplifies the troubleshooting process in the event of a malfunction and makes inspection and repair easier.

[0016] In one embodiment, as shown in FIGS. 2 and 3 , there are at least two sets of weighing units 12. The bracket 111 includes a mounting plate 1111 with mounting holes 1112 spaced apart (preferably evenly spaced apart). The number of mounting holes 1112 is the same as the number of weighing units 12. The load cells 122 are mounted on the mounting plate 1111 at positions facing the mounting holes 1112 using the mounting holder 121, and the hooks 123 pass through the mounting holes 1112 to connect to the load cells 122. That is, in this embodiment, each mounting hole 1112 is used to mount one set of weighing units 12. Note that the mounting holes 1112 may also be designed as grooves, and it is sufficient to allow the hooks 123 to pass through and connect to the load cells 122, while ensuring that the mounting holder 121g is firmly and securely attached to the mounting plate 1111. Further, the bracket 111 further includes a support lever 1113 connected to the mounting plate 1111. The support unit 11 further includes a front case 112 and a rear case 113. An attachment space for attaching the support lever 1113 is formed in an enclosed shape between the front case 112 and the rear case 113. That is, the support lever 1113 supports the mounting plate 1111 and all of the weighing units 12 attached to the mounting plate 1111, thereby maintaining the stability of the weighing units 12 during weighing. The front case 112 and the rear case 113 protect the support lever 1113. In one embodiment, the weighing unit 12 further includes a front cover 124 and a rear cover 125. An accommodation space for accommodating the mounting plate 1111, the mounting holder 121, and the load cell 122 is formed in an enclosed shape between the front cover 124 and the rear cover 125. That is, after the weighing unit 12 is mounted on the mounting plate 1111, the front cover 124 and the rear cover 125 further protect the mounting plate 1111 and the load cell 122 on the weighing unit 12 from being damaged in the accommodation space.

[0017] 4 and 5, the hook 123 includes a bent portion 1232, a lateral displacement portion 1233, and a latch portion 1234 connected to the load cell 122. The connecting screw 1231 may be a male thread attached to the latch portion 1234. Opposite ends of the bent portion 1232 are connected to the latch portion 1234 and the lateral displacement portion 1233, respectively. The bent portion 1232 and the lateral displacement portion 1233 form a locking ring having an opening 1235. The central axis L1 of the bent portion 1232 and the latch portion are both located on a first predetermined reference plane. The central axis of the lateral displacement portion 1233 is set at a predetermined lateral displacement angle a with respect to the first predetermined reference plane. In one embodiment, the bent portion 1232, the lateral displacement portion 1233, and the latch portion 1234 are integrally molded. This simplifies the manufacturing process of the hook 123, improves manufacturing efficiency, and reduces manufacturing costs. In some embodiments, the bent portion 1232, the lateral shift portion 1233, and the latch portion 1234 may be detachably connected. This facilitates storage and transportation, and even if a portion is damaged and needs to be replaced, only that portion needs to be replaced. The material of the hook 123 is preferably metal, such as stainless steel. The bending shape and bending angle of the bent portion 1232 can be set as needed. In this embodiment, the central axis L1 of the bent portion 1232 and the latch portion are both located in the same plane (i.e., both are located in a first predetermined reference plane, and further, when the hook 123 is detachably connected to the load cell 122, the first predetermined reference plane is perpendicular to the horizontal plane). In some embodiments, a portion of the central axis of the bent portion 1232 may be located in the first predetermined reference plane, and another portion may be bent to extend outside the first predetermined reference plane. The shape of the locking ring changes depending on the shapes of the lateral shift portion 1233 and the bent portion 1232, but there is no such restriction in the present invention, and when the sample 4 needs to be suspended from the locking ring, the sample 4 is suspended from the opening 1235 of the locking ring.Specifically, the connection point between the lateral deviation portion 1233 and the bent portion 1232 is located on a first predetermined reference plane. The lateral deviation portion 1233 is shifted laterally from this connection point in a direction away from the first predetermined reference plane. The distance between the lateral deviation portion 1233 and the first predetermined reference plane gradually increases in a direction away from the connection point and one end of the bent portion 1232. In the present invention, the lateral deviation portion 1233 does not necessarily have to be a straight line and may be a curved line (the central axis of the lateral deviation portion 1233 may be fitted to a straight line depending on the bending direction, etc.). It is sufficient that the lateral deviation portion 1233 is shifted in a direction away from the first predetermined reference plane and the shift angle reaches a predetermined shift angle.

[0018] In this embodiment, the sample 4 (not shown) may be hooked front-on within the locking ring from the position of the opening 1235 of the locking ring (with the wide side of the sample 4 facing the operation table of the cell treatment device). Furthermore, the central axis of the lateral offset portion 1233 is set at a predetermined lateral offset angle a with respect to a first predetermined reference plane. Therefore, when the sample 4 falls to the bottom of the locking ring along the lateral offset portion 1233 due to its own weight, it is naturally suspended sideways on the hook 123 (with the narrow side of the sample 4 facing the operation table). This narrows the width of the samples 4 arranged on the support unit 11 of the cell treatment device, allowing more samples 4 to be suspended on the same support unit 11, improving operational efficiency. Furthermore, during the process of suspending the sample 4 on the hook 123 of the present invention, the operator can obtain the sample 4 suspended sideways while the sample 4 is still suspended front-on, saving time and effort and significantly improving the user's operational experience.

[0019] Furthermore, the preset lateral displacement angle a is 30 to 60 degrees. Preferably, the preset lateral displacement angle a is 45 degrees. By setting the preset lateral displacement angle a within the above angle range, the operator can hang the sample 4 from the front of the operation table, improving the convenience of operation and allowing the sample 4 hung on the front to fall due to its own weight along the lateral displacement portion 1233 of the locking ring and be arranged sideways. This narrows the width occupied by the samples 4 arranged on the support unit 11 of the cell treatment device, allowing more samples 4 to be hung on the same support unit 11, improving operation efficiency.

[0020] In one embodiment, as shown in FIGS. 4 and 5, the central axis L1 of the hook portion penetrates the connection point between the bent portion 1232 and the laterally shifted portion 1233. The first relative distance is equal to or greater than the second relative distance. The first relative distance refers to the relative distance between the connection point and a second preset reference plane. The second preset reference plane is perpendicular to the hook portion 1234 and penetrates any part of the hook portion 1234. The second relative distance refers to the relative distance between a part other than the connection point of the bent portion 1232 and the laterally shifted portion 1233 and the second preset reference plane. After the hook 123 is attached to the cell treatment device, both the hook portion 1234 and the first preset reference plane on which it is located are perpendicular to the horizontal plane. Since the second preset reference plane is perpendicular to the hook portion 1234, the second preset reference plane in this case is a plane parallel to the horizontal plane, and the second reference plane penetrates any part of the hook portion 1234. For example, if the second reference plane penetrates an adjacent part of the hook portion 1234 and the bent portion 1232, the first relative distance is equal to or greater than the second relative distance, and therefore the connection point between the bent portion 1232 and the laterally displaced portion 1233 is the lowest point of the entire hook 123 in the current mounting state. Therefore, the sample 4 hooked on the hook 123 from the position of the opening 1235 of the locking ring falls naturally under its own weight and then drops into the connection point between the bent portion 1232 and the laterally displaced portion 1233. Furthermore, because the connection point is located directly below the central axis L1 of the hook, the hook portion 1234 of the hook 123 remains perpendicular to the horizontal plane even after the sample 4 slides into the connection position, ensuring the stability of the cell treatment device without shifting or swinging due to the gravity of the sample 4. Optionally, the bent portion 1232 may include a straight portion parallel to the central axis L1 of the hook. This arrangement makes the structure of the hook 123 stronger and more reliable.

[0021] 6 and 7, the centrifugal module 100 includes a rotating shaft 101, a bearing unit 102, a coupling 103, a drive motor 104 for rotating the rotating shaft 101, a brake 105 for braking the rotating shaft 101, and a rotary mounting seat 106 located in the centrifuge chamber for mounting the centrifugal cup. The coupling 103 is connected between the output end of the drive motor 104 and the rotating shaft 101. One end of the rotating shaft 101 remote from the coupling 103 passes through the bearing unit 102 and is connected to the rotary mounting seat 106. The brake 105 is connected to the bearing unit 102. The rotary mounting seat 106 is attached to the rotating shaft 101 by a pin 1012. Specifically, a pin hole 1013 is formed at one end of the rotating shaft 101 away from the coupling 103. The rotating mounting seat 106 is fixed to the rotating shaft 101 by a screw after a pin 1012 passing through the pin hole 1013 is inserted into the rotating mounting seat 106. A first locking portion is provided on the rotating mounting seat 106, and a second locking portion is provided on the centrifugal cup. The centrifugal cup is connected to the rotating mounting seat 106 by locking the first locking portion with the second locking portion. The locked centrifugal cup and the rotating mounting seat 106 are fixedly connected by a screw unit, thereby further strengthening the connection between them. The centrifugal module 100 is compatible with centrifugal cups of various specifications due to the installation of the rotating mounting seat 106. Furthermore, the drive motor 104 is a brushless DC motor, which extends the motor's service life and reduces noise. The coupling 103 is a diaphragm coupling. Since a diaphragm coupling does not have backlash, the use of a diaphragm coupling can further improve the transmission accuracy of the centrifugal module 100. The brake 105 brakes the rotating shaft 101 with a brake disc when the power is off, and releases the brake disc to release the braking state of the rotating shaft 101 when the power is on.In this case, the drive motor 104 operates to rotate the rotating shaft 101 via the coupling 103, which rotates the centrifugal cup attached to the rotating mounting seat 106, thereby performing cell processing such as separation, mixing, preparation, and washing of components of the sample 4 (e.g., blood) in the centrifugal cup.

[0022] In this embodiment, the rotating shaft 101 is rotatably mounted on the bearing unit 102, which limits the range of radial vibration of the rotating shaft 101 and reduces radial vibration when the centrifugal cup is rotated by the centrifugal module 100. The rotating shaft 101 is connected to the driving motor 104 by a coupling 103, and the braking and driving of the rotating shaft 101 are performed by a brake 105 and the driving motor 104. This simplifies the structure and improves transmission accuracy. Furthermore, in this invention, the rotating mounting base 106 attached to the rotating shaft 101 engages with and mounts the centrifugal cup, making the mounting of the centrifugal cup more convenient and secure, preventing the centrifugal cup from falling off when the rotating shaft 101 rotates the centrifugal cup via the rotating mounting base 106. This ensures the safety of the cell treatment process and improves the quality and effectiveness of cell treatment.

[0023] 7, the bearing unit 102 includes a bearing holder 1021 having a mounting through-hole and two bearings 1022 mounted on the inner wall of the mounting through-hole. The rotating shaft 101 is mounted on the bearing holder 1021 by passing through the two bearings 1022. The brake 105 is mounted on the bearing holder 1021. A through-hole 1051 is formed in the brake 105. The rotating shaft 101 passes through the through-hole 1051 and is rotatably connected to the bearing holder 1021 via the two bearings 1022. The brake 105 is fixedly mounted on the bearing holder 102 by screws, and the outer rings of the two bearings 1022 are attached to the inner wall of the mounting through-hole in a press fit within the bearing holder 1021. Optionally, the inner wall of the mounting through-hole may have two recessed, parallel annular grooves (not shown). The two bearings 1022 are fitted into the two annular grooves, respectively. The rotating shaft 101 passes through the through-hole 1051 of the brake 105, then passes through the two bearings 1022, and is rotatably connected to the bearing holder 1021 by the two bearings 1022. As an option, the rotating shaft 101 is provided with a positioning step 1011, and the two bearings 1022 are attached to the positioning step 1011. This allows the inner rings of the bearings 1022 to be stably fixed to the positioning step 1011. In this embodiment, the position of the rotating shaft 101 is restricted using the bearing unit 102 of the double bearing 1022, thereby maximizing the range of radial runout of the rotating shaft 101.

[0024] As shown in FIGS. 6 and 7 , the centrifugal module 100 further includes an irregular wheel 107 attached to the rotating shaft 101. The brake 105 is provided with a locking groove (not shown) that engages with the irregular wheel 107. The shape of the irregular wheel 107 can be set as needed. For example, the irregular wheel 107 may be a square wheel. In this case, the locking groove is provided as a square groove that engages with the square wheel. If the irregular wheel 107 disengages from the locking groove before braking by the brake 105, the square groove will not brake the rotating shaft 101 connected to the square wheel. After braking by the brake 105, the brake disc first brakes the rotating shaft 101. Then, the rotation speed of the rotating shaft 101 decreases, and the square wheel also fits into the locking groove as the rotating shaft 101 moves axially, quickly stopping the movement of the rotating shaft 101.

[0025] In one embodiment, as shown in FIGS. 6 and 7 , the centrifugal module 100 further includes a motor mounting seat 108 having a motor accommodating space 1081. The drive motor 104 is mounted in the motor accommodating space 1081. The bearing unit 102 is mounted on the motor mounting seat 108. Specifically, in this embodiment, the drive motor 104 is mounted in the motor accommodating space 1081 formed by a sink base opened in the motor mounting seat 108. A central shaft hole is formed in the inner wall of the motor accommodating space 1081, and the output end (i.e., output shaft) of the motor passes through the central shaft hole and is connected to the coupling 103. The drive motor 104 is then fixedly mounted in the sink base (e.g., connected by a screw). Furthermore, the centrifugal module 100 further includes a fixing plate 109 for connection to an external member. One end of the motor mounting seat 108 remote from the bearing holder 1021 is attached to the fixing plate 109. That is, the sink base opening end of the motor mounting seat 108 (the opening end of the motor accommodating space 1081) is connected and fixed to the fixing plate 109, so that the entire centrifugal module 100 together with this fixing plate 109 can be fixed to an external member, for example, fixed to the bottom of the centrifuge chamber of the centrifuge pot 21 of the cell processing device, and thereby the centrifuge cup is driven by the centrifugal module 100 to perform cell processing operations within the centrifuge chamber.

[0026] In one embodiment, as shown in FIGS. 8 and 9, the centrifuge device 2 further includes a temperature control module 22 for controlling the temperature of the centrifuge chamber. The temperature control module 22 includes a cooling unit 211 for cooling the centrifuge chamber and a heat dissipation unit 222 for dissipating heat from the cooling unit 211. The cooling surface of the cooling unit 211 is attached to the outer wall of the centrifuge pot 21. The heating surface of the cooling unit 211 is connected to the heat dissipation unit 222. The cooling unit 211 may also be at least one semiconductor cooling piece whose cooling surface is attached to the inner wall of the centrifuge pot 21. After being energized, the semiconductor cooling piece can cool the centrifuge chamber inside the centrifuge pot 21 using its cooling surface, resulting in a fast cooling rate and high cooling efficiency. The cooling unit 211 may also be a Peltier element. The cooling unit 211 can also realize a heating function, i.e., realize an operation mode opposite to the cooling mode, heating the cooling surface and cooling the surface opposite the cooling surface, thereby achieving the effect of supplying heat to the cooling unit 11. The centrifugal module 100 of the centrifugal device 2 performs cell processing by rotating the centrifuge cups located in the centrifuge chamber of the centrifuge pot 21. The temperature control module 22 can cool the centrifuge chamber using the cooling unit 211 attached to the outer wall of the centrifuge pot 21, thereby ensuring that the temperature range of the centrifuge cups for cell processing inside the centrifuge chamber can be controlled, and the cooling speed is fast, improving cooling efficiency. Furthermore, dissipating heat from the heating surface of the cooling unit 211 using the heat dissipation unit 222 prevents the temperature of the cooling unit 211 from rising as the cooling process progresses, avoiding the impact of the temperature rise of the cooling unit 211 on the cooling process, ensuring stability and accuracy of temperature control by the temperature control module 22 (the temperature control module 22 of the present invention has high temperature control accuracy, up to ±0.3°C, and the temperature controllable range is accurate, from 6°C to 37°C), and ensuring the constant temperature required for cell processing. Furthermore, the temperature control module 22 of the present invention has a simple structure, a small volume, and reduces device costs.

[0027] 8 and 9, the cooling unit 211 includes a first semiconductor cooling plate 2211 whose cooling surface is attached to the first side of the centrifuge pot 21. The heat dissipation unit 222 includes a cooling fan 2221, a heat dissipation plate 2222 connected to the heat-receiving surface of the first semiconductor cooling plate 2211, and a first ventilation pipe 2223 connected between the heat dissipation plate 2222 and the cooling fan 2221. By using the first semiconductor cooling plate 2211 for cooling, the cooling and heat dissipation structure is simpler than a water-cooled pipe. In this embodiment, the cooling surface of the first semiconductor cooling plate 2211 is first attached to the outer wall of the first side of the centrifuge pot 21 using thermally conductive silicone grease. Specifically, a first groove is formed in the outer wall of the first side of the centrifuge pot 21. When the first semiconductor cooling plate 2211 is attached to the first groove, the cooling energy dissipated during the cooling process is fully transferred to the interior of the centrifuge chamber. The heat sink 2222 is attached to the heat receiving surface of the first semiconductor cooling plate 2211 with thermally conductive silicone grease, and is pressed against the first semiconductor cooling plate 2211 while the heat sink 2222 is fixed to the outer wall of the centrifuge pot 21 with screws. Then, the first ventilation pipe 2223 is fixed to the heat sink 2222 with screws. Finally, the cooling fan 2221 is attached to one end of the first ventilation pipe 2223 remote from the heat sink 2222 and fixed with screws. As a result, after the first semiconductor cooling plate 2211 is energized, heat is dissipated from the heat receiving surface of the first semiconductor cooling plate 2211 by the heat sink 2222, the first ventilation pipe 2223, and the cooling fan 2221.

[0028] 8 and 9, a mounting portion 2224 is provided at one end of the first ventilation pipe 2223 away from the cooling fan 2221. The mounting portion 2224 is provided with a positioning groove 2225 that engages with the heat dissipation piece 2222. The mounting portion 2224 is attached to the outer wall of the centrifuge pot 21 with the heat dissipation piece 2222 positioned within the positioning groove 2225. That is, the provision of the positioning groove 2225 provides better protection for the heat dissipation piece 2222 when the heat dissipation piece 2222 is attached within the positioning groove 2225, and also strengthens the attachment of the first ventilation pipe 2223. Furthermore, the cooling fan 2221 further improves the heat dissipation effect of the heat dissipation piece 2222 via the first ventilation pipe 2223. The cooling unit 211 further includes a second semiconductor cooling piece 2212 whose cooling surface is attached to the second side of the centrifuge pot 21. The first side and the second side are disposed opposite each other. The heat dissipation unit 222 further includes a heat dissipation fan 2226, a second ventilation pipe 2227, and a heat pipe radiator 2228 connected to the heat-receiving surface of the second semiconductor cooling plate 2212. One end of the heat pipe radiator 2228 is connected to the heat dissipation fan 2226. The other end of the heat pipe radiator 2228, facing the heat dissipation fan 2226, is connected to the inlet of the second ventilation pipe 2227. The cooling units 211 may be attached to multiple sides of the centrifuge pot 21 on approximately the same plane and distributed in a circular pattern, thereby forming a closed cooling circle. This cooling circle can rapidly reduce the temperature at the center of the plane, accelerating the cooling effect at the center.

[0029] That is, in this embodiment, as shown in FIGS. 8 and 9, the first semiconductor cooling plate 2211 and the second semiconductor cooling plate 2212 are symmetrically attached to the outer wall of the centrifuge pot 21, thereby further accelerating the cooling rate. The cooling surface of the second semiconductor cooling plate 2212 is first attached to the second outer wall of the centrifuge pot 21 with thermally conductive silicone grease. Specifically, a second groove is formed in the second outer wall of the centrifuge pot 21, and the second semiconductor cooling plate 2212 is attached to the second groove, thereby efficiently transferring the cooling energy during the cooling process to the inside of the centrifuge chamber. The heat pipe radiator 2228 is attached to the heat-receiving surface of the second semiconductor cooling plate 2212 with thermally conductive silicone grease, and the second semiconductor cooling plate 2212 is pressed against the heat pipe radiator 2228, which is then fixed to the outer wall of the centrifuge pot 21 with screws. A heat dissipation fan 2226 is installed above the heat pipe radiator 2228. The second ventilation pipe 2227 is fixed to the centrifugal pot 21. The intake port of the second ventilation pipe 2227 is located above the heat pipe radiator 2228 and is installed facing the heat dissipation fan 2226. This allows the cooling fan 2226 to use the flowing air to dissipate heat from the heat pipe radiator.

[0030] 8 and 9, the temperature control module 22 further includes a temperature sensor 224 attached to the bottom of the centrifuge chamber of the centrifuge pot 21. The temperature sensor 224 is a high-precision temperature sensor inserted into the centrifuge chamber and fixed to the bottom of the centrifuge chamber to detect the temperature of the air inside the centrifuge chamber in real time during the cell processing process. The temperature sensor 224 is fixed with a thermally conductive adhesive and can feed back the real-time monitored temperature inside the centrifuge pot 21 to the control panel of the cell processing device. The control panel controls the current of the cooling unit 211 (e.g., the first semiconductor cooling piece 2211 and the second semiconductor cooling piece 2212) in response to the temperature to maintain the temperature inside the centrifuge pot 21 within a preset range.

[0031] In one embodiment, as shown in FIGS. 8 and 9, a drain port (not shown) is provided at the bottom of the centrifuge pot 21. The centrifuge device 2 further includes a liquid detection sensor 25 (fixed with a sealant) installed at the bottom of the centrifuge chamber of the centrifuge pot 21 and a drain joint 23 connected to the drain port. The liquid detection sensor 25 can monitor the amount of liquid remaining inside the centrifuge chamber in real time. For example, when the liquid detection sensor 25 detects the presence of a certain amount of liquid at the bottom of the centrifuge chamber, the controller activates a signal in response to the detection signal received from the liquid detection sensor 25 and opens the drain joint 23 to drain the liquid through the drain port and the drain joint 23. The drain joint 23 is circumscribed by an inlet pipe and drains liquid (e.g., condensed water) from the centrifuge chamber through the drain port, drain joint, and inlet pipe, in that order, preventing liquid accumulation or leakage.

[0032] 1, 8, and 9, a connection flange 212 is provided on the edge of the centrifuge chamber of the centrifuge pot 21. The centrifuge device 2 further includes a seal ring 24 bonded to the connection flange 212 and a flip cover 26 rotatably connected to the housing 5. The flip cover 26 is installed on the edge of the centrifuge chamber and engages with the seal ring 24 to open and close the centrifuge chamber. That is, the seal ring 24 is adhesively fixed to the connection flange on the top of the centrifuge pot 21, and engages with the flip cover 26 of the centrifuge device 2 to easily seal the centrifuge chamber, ensuring safety and airtightness of the cell treatment process and preventing cold air from leaking to the outside. The flip cover 26 may be opened before the cell processing device starts operating, but after the liquid in the sample 4 is consumed (after it has been completely poured into the centrifuge cup and cell processing has been performed), operations such as inserting a pipe or replacing the centrifuge cup become necessary, and the flip cover 26 may be closed again until it is attached to the sealing ring 24 to seal the centrifuge chamber, thereby fulfilling the role of safety protection and maintaining the temperature inside the centrifuge chamber.

[0033] In one embodiment, as shown in FIG. 1 , the cell treatment device further includes a controller (not shown) mounted within the housing 5, a scanning device 6 for scanning and identifying the markings corresponding to the sample 4, and a display device 7 for touch operation or parameter display. The metering unit 12, the centrifuge 2, the liquid path module 3, the scanning device 6, and the display device 7 are all connected to the controller. The display device 7 allows the user to set, modify, and monitor relevant parameters in the cell treatment process by touching a man-machine interaction display interface. The display device 7 is rotatably connected to the housing 5 (e.g., rotatably connected to the housing 5 by a hinge), which facilitates storage and reduces the device volume. The rotation angle of the display device 7 can be adjusted according to the operator's visual angle needs, improving the user experience. The shape and size of the display device 7 can be customized as needed. The scanning device 6 scans and identifies the identification code on the sample 4, and transmits the data information obtained through the identification to the controller for use. The size and shape of the scanning device 6 can be customized as needed.

[0034] 1 and 10 , the conduit unit 31 includes a pinch valve 311 for controlling the opening and closing of the conduit in the conduit unit 31, an air bubble sensor 312 for monitoring in real time whether air bubbles are present in the liquid flowing through the conduit and determining whether all of the liquid in the sample 4 has flowed, a pressure sensor 313 for monitoring in real time the pressure of the liquid in the conduit, a peristaltic pump 314 for flowing the liquid in the conduit during operation, and a step motor 315 whose output end is connected to the peristaltic pump 314 for operating the peristaltic pump 314. The pinch valve 311, the air bubble sensor 312, the pressure sensor 313, and the peristaltic pump 314 are all attached to the front surface of the liquid path panel 32. The step motor 315 is attached to the back surface of the liquid path panel 32 at a position facing the peristaltic pump 314, and is located within the housing 5. That is, the pinch valve 311, the bubble sensor 312, the pressure sensor 313, and the peristaltic pump 314 are attached to the front of the liquid path panel 32 and secured with screws. The step motor 315 is attached to the rear of the liquid path panel 32 and secured with screws. The peristaltic pump 314 is attached above the step motor 315, positioned in front of the liquid path panel 32, and secured with screws. In addition to the above components, the conduit unit 31 further includes conduits connected between each component. When the cell processing device is in operation, the step motor 315 rotates the peristaltic pump 314 as a power source for the flow of liquid in the sample 4, thereby causing the liquid to flow. The pinch valve 311 (one or more pinch valves can be installed as needed, and can be switched by an electromagnetic switch) functions as a switch, controlling the flow of liquid in the conduit for transporting the liquid in the sample 4 and changing the flow path. The air bubble sensor 312 (one or more can be installed as needed) monitors in real time whether or not air bubbles are present in the fluid in the pipeline, and uses the monitoring results as a criterion for determining whether all of the liquid in the sample 4 has been used.A pressure sensor 313 (one or more sensors can be installed as needed) monitors the liquid pressure in the pipelines in real time. If the pressure exceeds a preset pressure threshold, the controller receives a pressure signal from the pressure sensor 313 and then sends a feedback signal to automatically adjust the pipeline unit 31 or notify the operator of the cell processing device to make adjustments. In the present invention, the pipeline unit 31 is openly laid out on the pipeline panel 32, which can be mounted on the housing 5 at a 125° inclination, facilitating operation, installation, and removal. The pipeline panel 32 is also equipped with a pressure sensor 313 to monitor the pressure in the pipelines and prevent clogging. The pipeline panel 32 is further equipped with an air bubble sensor 312 to monitor the air bubble status in the pipelines and determine whether the liquid in the sample 4 has been used up, making the use process more convenient.

[0035] In one embodiment, as shown in FIGS. 1 and 10 , when the start button 51 on the housing 5 is pressed, the cell processing device waits a few seconds before entering an operating state. At this time, relevant parameters are set using the man-machine interaction display interface of the display device 7, the scanning device 6 scans and identifies the identification code of the sample 4, and the data information is obtained and transmitted to the controller, completing the data information entry. Then, the pinch valve 311 on the liquid path panel 32 is opened using the man-machine interaction display interface of the display device 7, and the sample 4 is loaded. The sample 4 is then hung on the hook 123 of the corresponding metering device 1, and the pipelines are connected to the pinch valve 311, pressure sensor 313, air bubble sensor 312, and other components mounted on the liquid path panel 32. The centrifuge cup is then attached to the rotating mounting base 106 of the centrifuge module 100, and the flip cover 26 is closed to seal the centrifuge chamber. After confirming that the sample 4 is correctly installed, the start button on the man-machine interaction display interface is pressed to start cell processing of the liquid sample 4 flowing into the centrifuge cup. After cell processing is complete, the pinch valve 311 on the liquid path panel 32 is opened to remove the sample 4, completing the process. The cell processing device of the present invention is small in volume and occupies a small area, making it easy to install and transport. It also has a precise temperature adjustment range (4°C to 40°C) and high temperature control accuracy (±0.3°C). Furthermore, this cell processing device consumes little energy, has a long life, and is quiet.

[0036] The above is merely an example of the cell treatment device of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention are all included in the protection scope of the present invention. [Explanation of symbols]

[0037] 1 Weighing device 11 Support unit 111 Bracket 1111 Mounting plate 1112 Mounting hole 1113 Support lever 112 Front Case 113 Rear case 12 Weighing Unit 121 Mounting holder 1211 Storage groove 1212 Fixed hole 1213 flange 122 load cells 123 Hook 1231 Connection screw 1232 Bend section 1233 Strike-slip area 1234 Hook stopper 12341 Connection 1235 Aperture a Preset side-slip angle L1 Central axis of the latch 124 Front cover 125 rear cover 2. Centrifugal device 21 Centrifugal pot 212 Connection flange 22 Temperature Control Module 221 Cooling Unit 2211 First semiconductor cooling piece 2212 Second semiconductor cooling piece 222 Heat dissipation unit 2221 Cooling fan 2222 Heat sink 2223 First ventilation pipe 2224 Mounting part 2225 Positioning groove 2226 Heat dissipation fan 2227 Second ventilation pipe 2228 Heat Pipe Radiator 223 Heat retention layer 224 Temperature Sensor 23 Drainage joint 24 Seal ring 25 Liquid detection sensor 26 Flip Cover 100 Centrifugal Module 101 Rotation axis 1011 Positioning step 1012 pins 1013 Pinhole 102 Bearing unit 1021 Bearing holder 1022 Bearing 103 Coupling 104 Drive motor 105 Brake 1051 Through hole 106 Rotating mounting base 107 Irregular Wheel 108 Motor mounting seat 1081 Motor storage space 109 Fixed plate 3 Liquid Path Module 31 Pipe unit 311 Pinch valve 312 Air Bubble Sensor 313 Pressure Sensor 314 Peristaltic Pump 315 Step Motor 32 Liquid path panel 4 Sample 5. Housing 51 Start button 6. Scanning Device 7 Display device

Claims

1. A cell treatment device comprising: a weighing device including a support unit and at least one set of weighing units, the support unit including a bracket for supporting the weighing units, and each set of the weighing units including a mounting holder attached to the bracket, a load cell attached to the mounting holder, and a hook detachably attached to the load cell for suspending a sample; a centrifugal device including a centrifuge pot having a centrifuge chamber, a centrifuge cup installed in the centrifuge chamber, and a centrifuge module connected to the centrifuge pot for rotating the centrifuge cup; a liquid path module including a pipeline unit for transporting the liquid in the sample into the centrifuge cup for cell processing, and a liquid path panel for mounting the pipeline unit and controlling the flow of the liquid in the pipeline unit; a housing to which the centrifuge pot, the liquid path panel, and the bracket are all attached.

2. The metering units are in at least two sets, the bracket includes a mounting plate having spaced apart mounting holes; The number of the mounting holes is the same as that of the measuring unit, the mounting holder mounts the load cell at a position facing the mounting hole of the mounting plate; The cell treatment device according to claim 1 , wherein the hook passes through the mounting hole and is connected to the load cell.

3. the hook includes a bent portion, a lateral displacement portion, and a hook portion connected to the load cell; the opposite ends of the bent portion are connected to the hook portion and the lateral displacement portion, respectively; a locking ring having an opening is formed in a surrounding manner by the bent portion and the laterally displaced portion, the central axes of the bent portion and the hook portion are both located on a first preset reference plane; The cell treatment device according to claim 1 , wherein the central axis of the lateral displacement portion is set at a predetermined lateral displacement angle with respect to the first predetermined reference plane.

4. the centrifugal module includes a rotating shaft, a bearing unit, a coupling, a drive motor for rotating the rotating shaft, a brake for braking the rotating shaft, and a rotating mounting seat located within the centrifuge chamber for mounting the centrifugal cup; the coupling is connected between an output end of the drive motor and the rotary shaft, an end of the rotating shaft remote from the coupling passes through the bearing unit and is connected to the rotating mounting seat; The cell treatment device according to claim 1 , wherein the brake is connected to the bearing unit.

5. The bearing unit includes a bearing holder having a mounting through-hole, and two bearings mounted on the inner wall of the mounting through-hole; the rotating shaft passes through the two bearings and is attached to the bearing holder; The brake is attached to the bearing holder, the centrifugal module further includes an irregular wheel attached to the rotating shaft; The cell treatment device according to claim 4, wherein the brake is provided with a locking groove that engages with the irregular wheel.

6. the centrifuge device further includes a temperature control module for controlling the temperature of the centrifuge chamber; the temperature control module includes a cooling unit for cooling the centrifuge chamber and a heat dissipation unit for dissipating heat from the cooling unit, The cooling surface of the cooling unit is attached to the outer wall of the centrifuge pot; The cell treatment device according to claim 1 , wherein the heating surface of the cooling unit is connected to the heat dissipation unit.

7. the cooling unit includes a first semiconductor cooling piece having a cooling surface attached to a first side of the centrifuge pot; the heat dissipation unit includes a cooling fan, a heat dissipation piece connected to the heat receiving surface of the first semiconductor cooling piece, and a first ventilation pipe connected between the heat dissipation piece and the cooling fan; the cooling unit further includes a second semiconductor cooling piece having a cooling surface attached to a second side of the centrifuge pot; The first side and the second side are disposed opposite each other, the heat dissipation unit further includes a heat dissipation fan, a second ventilation pipe, and a heat pipe radiator connected to the heat receiving surface of the second semiconductor cooling piece; One end of the heat pipe radiator is connected to the heat dissipation fan; The cell treatment device according to claim 6, wherein the other end of the heat pipe radiator, which faces the heat dissipation fan, is connected to an intake port of a second ventilation pipe.

8. a connecting flange is provided on the edge of the centrifuge chamber of the centrifuge pot; The centrifugal device further includes a seal ring bonded to the connection flange and a flip cover rotatably connected to the housing, The cell treatment device according to claim 1, wherein the flip cover is installed on the edge of the centrifuge chamber and engages with the seal ring to open and close the centrifuge chamber.

9. a controller mounted within the housing; a scanning device for scanning and identifying indicia corresponding to the sample; and a display device for touch operation or parameter display; The cell treatment device according to claim 1 , wherein the measuring unit, the centrifugal device, the liquid path module, the scanning device, and the display device are all connected to the controller.

10. the conduit unit includes a pinch valve for controlling opening and closing of the conduit in the conduit unit, an air bubble sensor for monitoring in real time whether air bubbles are present in the liquid flowing in the conduit and determining whether all of the liquid in the sample has flowed, a pressure sensor for monitoring in real time the pressure of the liquid in the conduit, a peristaltic pump for causing the liquid in the conduit to flow during operation, and a step motor whose output end is connected to the peristaltic pump for operating the peristaltic pump, the pinch valve, the air bubble sensor, the pressure sensor, and the peristaltic pump are all attached to a front surface of the liquid path panel; The cell treatment device according to claim 1, wherein the step motor is attached to a rear surface of the liquid path panel at a position opposite to the peristaltic pump and is located within the housing.

Images