Cultivation medium for *Lyophyllum decastes*, cultivation method for *Lyophyllum decastes*

Abstract

This provides a novel technique for cultivating *Lyophyllum decastes*. [Solution] A growing medium for cultivating Lyophyllum decastes, containing coffee grounds.

Description

Technical Field

[0001] The present invention relates to the cultivation of Lyophyllum decastes.

Background Art

[0002] Lyophyllum decastes is a kind of mushroom belonging to the Tricholomataceae family and is used as food. As a cultivation method of Lyophyllum decastes, for example, the method described in Patent Document 1 has been proposed.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] One of the objects of the present invention is to provide a novel technique for the cultivation of Lyophyllum decastes.

Means for Solving the Problems

[0005] The inventor conceived of cultivating Lyophyllum decastes using a novel culture medium. As a result of intensive research, the inventor found that Lyophyllum decastes can be cultivated in bed log cultivation using a culture medium containing coffee grounds.

[0006] The gist of the present invention is as follows. [1] A culture medium for cultivating Lyophyllum decastes, containing coffee grounds. [2] A method for cultivating Lyophyllum decastes, including cultivating Lyophyllum decastes using a culture medium containing coffee grounds.

Effects of the Invention

[0007] According to the present invention, a novel technique for cultivating Lyophyllum decastes can be provided. [Modes for carrying out the invention]

[0008] One embodiment of the present invention will be described in detail below. This embodiment relates to the cultivation of Lyophyllum decastes, and involves cultivating Lyophyllum decastes in a culture medium containing coffee grounds.

[0009] As mentioned above, Lyophyllum decastes is a species of mushroom belonging to the family Lyophyllaceae. In this embodiment, specifically, the mycelium (spawn) of Lyophyllum decastes is inoculated into a culture medium containing coffee grounds and cultured.

[0010] In this specification, coffee grounds refer to the residue generated after roasted coffee beans have been ground and coffee has been extracted from the resulting powder. There are no particular restrictions on the variety of coffee beans used as the raw material for the coffee grounds in this embodiment; for example, Arabica, Robusta, and Liberica varieties can be used. Furthermore, there are no particular restrictions on the origin of the coffee beans. The raw material for the coffee grounds may be a single variety or single origin coffee bean, or a mixture of multiple varieties or origins.

[0011] There are no particular limitations on the method of extracting coffee from coffee beans, roasting temperature, or roasting environment. For example, roasting methods include direct-fire, hot-air, and semi-hot-air. The degree of roasting of the coffee beans is also not particularly limited, and can include light, cinnamon, medium, high, city, full city, French, and Italian. Furthermore, the type of solvent used for extraction is not particularly limited, but water is one example.

[0012] In this embodiment, the coffee grounds can be used either in their moist state immediately after brewing or after being dried. If dried, the drying method is not particularly limited and can include forced drying using a hot air convection dryer or natural drying in the sun.

[0013] Coffee grounds act in the culture medium, for example, as a culture medium base material. In this embodiment, the content of coffee grounds in the culture medium is not particularly limited and can be, for example, 10% by mass or more and 100% by mass or less, and is preferably 20% by mass or more and 70% by mass or less from the viewpoint of promoting the growth of Lyophyllum decastes.

[0014] The culture medium according to this embodiment may contain other components in addition to coffee grounds, as long as they can achieve the objectives of the present invention, and is not particularly limited. Other components include, for example, water, components that act as a culture medium, and nutrients. Examples of components that act as culture medium substrates include corn cob meal, sawdust, sawdust, chips, and chip dust. Examples of nutritional supplements include corn bran, corn cobs, rice bran, sugars, wheat bran, brewer's meal, dried brewer's yeast, tofu meal, peanut meal, rapeseed meal, cottonseed meal, potato protein, corn gluten feed, soybean meal, soybean meal, protein, minerals, vitamins, and more.

[0015] The culture medium of this embodiment can be prepared by mixing coffee grounds and other components as needed. There are no particular restrictions on the pH or moisture content of the culture medium; for example, a pH of 4.0 to 7.0 and a moisture content of 40 to 80% would suffice.

[0016] The cultivation of *Lyophyllum decastes* in this embodiment can be carried out by cultivating *Lyophyllum decastes* using the culture medium of this embodiment. Furthermore, other conditions may be known conditions used in the cultivation of *Lyophyllum decastes* on substrates, and are not particularly limited. For example, after performing a sterilization treatment (e.g., autoclaving at 121°C for 60 minutes in a medium), inoculate the culture medium with the inoculum of Pleurotus eryngii. Thereafter, culture the mycelium in the dark at 15 - 25°C and a humidity of 50 - 100% for about 0.5 - 1.5 months, and perform cultivation at 15 - 25°C and a humidity of 50 - 100% for about 0.5 - 1.5 months from those in which the mushroom (fruiting body) has formed under light conditions. Also, in the stage of culturing the mycelium, treatments such as temperature reduction and lack of bacteria may be performed to promote the formation of the fruiting body.

[0017] As described above, according to the present embodiment, a novel technique for cultivating Pleurotus eryngii can be provided.

Example

[0018] Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.

[0019] [Preparation of a medium containing coffee grounds] 800 g of roasted coffee powder was added to 8000 g of hot water at 95°C. After sufficient water supply for 20 minutes, extraction was performed. After extraction, filtration was carried out using a 40 - mesh sieve to obtain coffee grounds. The weight of the coffee grounds after filtration was measured, and the moisture content was calculated using the following formula. Moisture content = (weight after filtration (g) - amount of coffee powder (g)) / weight after filtration (g) × 100 (%) Thereafter, water was added to adjust the moisture content to 60% to obtain a medium.

[0020] [Cultivation of Pleurotus eryngii and other mushrooms] The obtained medium was placed in a polypropylene container (I - Boy wide - mouth bottle (250 ml) manufactured by AS ONE Corporation), and autoclaving was performed at 121°C for 60 minutes in the medium. After the sterilization treatment, the mycelium of the mushrooms shown in Table 1 was inoculated into the medium, and cultivation was carried out under the condition of 23°C. The humidity conditions were as follows. Enoki mushroom: around 90% Shiitake mushroom: 70 - 80% Hairy wood ear mushroom: 60-90% Tamogitake mushroom: 85-95% Hatake Shimeji: 90% or more Willow mushroom: 80-90% Pleurotus ostreatus: 70-90% Himalayan oyster mushroom: 70-90% Mukitake mushrooms: 70-90% Beech mushroom: 70-90% 30 to 60 days after the start of cultivation, we determined whether or not normal fruiting bodies were obtained. In Table 1, ○ indicates that normal fruiting bodies were obtained, and × indicates that the fruiting bodies were malformed or had poor growth.

[0021] [Table 1]

[0022] As can be seen from Table 1, when *Lyophyllum decastes* was cultivated using the culture medium of the example, normal fruiting bodies were obtained. Furthermore, when cultivation of *Lyophyllum decastes* was continued, *Lyophyllum decastes* was obtained at the yields shown in Table 2. *Fungal spread days* refers to the number of days required from the start of cultivation until the mycelium spread throughout the entire culture medium and was deemed sufficiently widespread, while *peak days* refers to the number of days from the start of cultivation until the day with the highest daily yield.

[0023] [Table 2]

Claims

1. A growing medium for cultivating Lyophyllum decastes, containing coffee grounds.

2. A method for cultivating Lyophyllum decastes, including cultivating Lyophyllum decastes using a culture medium containing coffee grounds.

Images