Diarrhea-suppressing material for dogs

Abstract

This product provides a diarrhea-suppressing material that uses common food ingredients without complex processing or treatment, and without deviating from the palatability of dogs, thereby preventing diarrhea and loose stools in dogs. [Solution] A poorly soluble soy protein material, characterized by a leucine (Leu) to glutamic acid (Glu) mass ratio of 2 or less and an NSI of 10-60%, can suppress diarrhea and loose stools in dogs with small amounts of intake. This material can be obtained as an undegraded residue from the soy peptide manufacturing process.

Description

Technical Field

[0001] The present invention relates to a diarrhea suppression material for dogs and a method for suppressing diarrhea in dogs, which can suppress diarrhea in dogs.

Background Art

[0002] Some dogs may have "diarrhea"-like excretion with soft feces. This can be caused not only by diseases, but also by mental factors such as stress, overeating, or gastrointestinal irritation caused by eating unfamiliar foods. In recent years, due to the extended lifespan of dogs, diarrhea caused by weakened digestive function due to aging is also frequently seen. In particular, many dogs are trained to defecate during walks, and it is difficult for owners to collect diarrhea feces when disposing of the feces as garbage. There are dietary and medicinal approaches to improving diarrhea in dogs. In terms of diet, in addition to regular dog food, it is recommended to give sweet potatoes, okara, and pumpkins rich in dietary fiber. Also, dog foods that claim to prevent and improve diarrhea are sold. Medicines include animal pharmaceuticals with the efficacy of stopping diarrhea. Patent Document 1 relates to the prevention and treatment of diarrhea in dogs using intestinal resident bacteria. Patent Document 2 relates to the improvement of diarrhea and soft stools in animals including dogs by adding galactomannan and boron.

[0003] Soybean protein materials are common food materials, and soybean peptides obtained by enzymatic hydrolysis of these with protease are also widely used due to effects such as easy absorbability. Also, it is known that the precipitate fraction generated by enzymatic decomposition of soybean protein has a cholesterol-lowering effect (Patent Document 3).

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Patent Document 2

[0005] Some dogs may not like fiber-rich feed and will not eat it. The technology in Patent Document 1 requires considerable effort to process and prepare lactic acid bacteria. The technology in Patent Document 2 involves treating galactomannan with boron and similarly requires considerable effort. The present invention aims to provide a diarrhea-suppressing material that suppresses diarrhea and loose stools in dogs without complex processing or treatment, using common food ingredients, and without deviating from the dog's palatability. [Means for solving the problem]

[0006] As a result of diligent research into the above-mentioned problems, the inventors have discovered that using a poorly soluble soy protein material having a characteristic amino acid composition in which the mass ratio of glutamic acid (Glu) to leucine (Leu) is 2 or less, and an NSI of 10-60%, can suppress diarrhea and loose stools in dogs with small amounts of intake. Furthermore, they have found that this material can be obtained from the undegraded residue of the soy peptide manufacturing process, thus completing the present invention.

[0007] In other words, the present invention (1) A diarrhea suppressant for dogs, in which a poorly soluble soy protein material is used as the active ingredient, having an amino acid composition in which the mass ratio of glutamic acid (Glu) to leucine (Leu) is 2 or less, and the NSI is 10-60%. (2) The poorly soluble soy protein material is an undegraded residue produced by enzymatic hydrolysis of soy protein raw materials, as described in (1), for use in dogs to suppress diarrhea. (3) A diarrhea-suppressing material for dogs, as described in (1) or (2), for oral administration of 0.4 g / kg / day or more as a poorly soluble soy protein material. (4) A method for producing a diarrhea-suppressing material for dogs, comprising enzymatic hydrolysis of a soy protein raw material and recovery of the resulting undegraded residue as a poorly soluble soy protein material. (5) A method for producing a diarrhea-suppressing material for dogs as described in (4), wherein the poorly soluble soy protein material has an amino acid composition in which the mass ratio of glutamic acid (Glu) to leucine (Leu) is 2 or less, and the NSI is 10-60%. (6) A method for producing a diarrhea-suppressing material for dogs, as described in (4) or (5), for oral administration of 0.4 g / kg / day or more of poorly soluble soy protein material. (7) A method for suppressing diarrhea in dogs, using a poorly soluble soy protein material having an amino acid composition in which the mass ratio of glutamic acid (Glu) to leucine (Leu) is 2 or less, and the NSI is 10-60%. (8) A method for suppressing diarrhea in dogs as described in (7), comprising orally administering 0.4 g / kg / day or more of poorly soluble soy protein material. (9) A method for suppressing diarrhea in dogs according to (7) or (8), wherein the poorly soluble soy protein is an undegraded residue produced by enzymatic hydrolysis of a soy protein raw material. (10) Dog feed containing poorly soluble soy protein material having an amino acid composition in which the mass ratio of glutamic acid (Glu) to leucine (Leu) is 2 or less, and the NSI is 10-60%. (11) A dog feed as described in (10), wherein the poorly soluble soy protein material is an undegraded residue produced by enzymatic hydrolysis of a soy protein raw material. (12) A dog feed according to (10) or (11), which contains 0.5% by mass or more of poorly soluble soy protein material in dry matter. (13) A wet-type feed for dogs according to (10) or (11), which has a moisture content of 75% by mass or more and contains 8% by mass or more of poorly soluble soy protein material in dry matter. (14) A drug for suppressing diarrhea in dogs, comprising a poorly soluble soy protein material as the active ingredient, having an amino acid composition in which the mass ratio of glutamic acid (Glu) to leucine (Leu) is 2 or less, and the NSI is 10-60%. (15) A pharmaceutical for suppressing diarrhea in dogs, as described in (14), wherein the poorly soluble soy protein material is an undegraded residue produced by enzymatic hydrolysis of soy protein raw materials. (16) A drug for suppressing diarrhea in dogs, as described in (14) or (15), for oral administration of 0.4 g / kg / day or more of poorly soluble soy protein material. This concerns... [Effects of the Invention]

[0008] According to the present invention, a material that effectively suppresses diarrhea and loose stools in dogs can be obtained. Since this material is effective in small amounts, it can be easily administered in the form of dog food or other feed. [Modes for carrying out the invention]

[0009] (Poorly soluble soy protein material) The poorly soluble soy protein material of the present invention has an amino acid composition in which the mass ratio of Glu to Leu is 2 or less, and the soluble nitrogen index (NSI), described later, is 10-60%. Typically, it is obtained as a precipitate fraction from the undegraded residue generated during the process of degrading the soy protein raw material with protease in the industrial production of peptides using soy protein as a raw material. Because the main product, the peptide, belongs to the low molecular weight fraction, this precipitate fraction is sometimes referred to as "HMF" (High Molecular Weight Fraction), which means the high molecular weight fraction. The manufacturing method will be explained below with examples.

[0010] (Soy protein source) Soy protein raw materials are made by concentrating protein from soybeans, and include concentrated soy protein and isolated soy protein. Soy protein raw materials may be sterilized and dried. Soy protein raw materials preferably contain 80% or more protein by dry weight, and isolated soy protein is preferred. Isolated soy protein is generally prepared by the following method: Water is added to defatted soybeans, extraction is performed at a near-neutral pH, and okara (soy pulp) is separated to obtain soy milk. Next, the soy milk is adjusted to a pH of around 4.5, and the isoelectric point precipitate is collected. Water and an alkaline agent are added to the precipitate to obtain an aqueous solution with a solid content concentration of 5-15% by mass and a pH of 5.7-8.0, preferably around 6.8-7.5. The isolated soy protein obtained in this way may be used as is in the following steps, or it may be used after adding water to the isolated soy protein powder. Isolated soy protein is not limited to the above method, and various modifications of this method may be used. Examples of commercially available soy protein include "Fujipro-R" and "Fujipro-SE" (manufactured by Fuji Oil Co., Ltd.).

[0011] (Hydrolysis) The following describes a method for obtaining hydrolyzed soy protein material, which is a poorly soluble fraction, from soy protein raw materials by enzymatic hydrolysis. The degree of enzymatic hydrolysis can be appropriately determined for the peptide, which is the main product of the degradation process, while considering the characteristics to be imparted to the peptide. Generally, it is appropriate that not all molecules are broken down to free amino acids, and a higher degree of degradation is preferable. Examples include soy protein hydrolysates in which the content of peptide fractions with a molecular weight of less than 500 is preferably 50% by mass or more, more preferably 60% by mass or more, and even more preferably 65% ​​by mass or more, relative to the total amount of peptides and free amino acids.

[0012] The proteases used for enzymatic hydrolysis to obtain soy protein hydrolysates can be appropriately selected from among those classified as "metalloproteases," "acid proteases," "thiol proteases," and "serine proteases," regardless of whether they are of animal, plant, or microbial origin. Preferably, they are those classified as "metalloproteases," "thiol proteases," and "serine proteases." Furthermore, a method of sequentially or simultaneously using two or more, or three or more, different classifications of enzymes is also efficient and preferable.

[0013] This classification of proteases is a classification method based on the types of amino acids in the active center, which is commonly used in the field of enzyme science. As representatives of each type, "metallo-proteases" include neutral proteases derived from Bacillus, Streptomyces, and Aspergillus, thermolysin, etc.; "acid proteases" include pepsin, acid proteases derived from Aspergillus, sumizyme FP, etc.; "thiol proteases" include bromelain, papain, etc.; and "serine proteases" include trypsin, chymotrypsin, subtilisin, alkaline protease derived from Streptomyces, alcalase, bioprase, etc.

[0014] The reaction pH and reaction temperature for protease treatment can be set according to the characteristics of the protease used. Usually, the reaction pH is around the optimum pH, and the reaction temperature is around the optimum temperature. Generally, the reaction temperature is 20 - 80°C, preferably 40 - 60°C. After the reaction, it is heated to a temperature sufficient to inactivate the enzyme (about 六十 - 一百七十°C) to inactivate the residual enzyme activity.

[0015] (Separation and drying) From the reaction solution after protease treatment, the insoluble fraction, which is the undigested residue generated by hydrolysis, is recovered by centrifugation or filtration to obtain an insoluble soy protein material. The recovered insoluble soy protein material is usually sterilized and then used in the form of a dry powder obtained by spray drying, freeze drying, etc. Sterilization is preferably by heat sterilization, with a heating temperature preferably of 110 - 170°C, more preferably 130 - 170°C. The heating time is preferably 3 - 20 seconds. Also, the reaction solution can be adjusted to an arbitrary pH. By performing a refinement treatment such as wet grinding before drying, a material with a better texture can also be obtained.

[0016] (Properties of the insoluble soy protein material) It should be noted that there seems to be an unclear expression "六十 - 一百七十°C" in the translation of item . It might be a mistake in the original text. If it is supposed to be a specific temperature range like "60 - 170°C", it should be corrected in the original text for a more accurate translation.The insoluble soy protein material obtained by the steps exemplified above has a soluble nitrogen index (NSI) of 10 to 60%, preferably 20 to 50%. It has an amino acid composition with a mass ratio of Glu to Leu of 2 or less, preferably 1.7 or less, more preferably 1.4 or less, and most preferably 1.2 or less. As the amount of amino acids in the protein, Glu can be exemplified as 16% by mass or less. Preferably, it is 14 to 8% by mass. Also, Leu can be exemplified as 9% by mass or more. Preferably, it is 10 to 18% by mass. The TCA solubility rate described later is preferably 1 to 50%, more preferably 10 to 45%. Although there are many unclear points about the principle by which the insoluble soy protein material of the present invention suppresses diarrhea and soft stools in dogs, an effect is recognized in the soy protein material having the compositional physical properties within the above range. In the present invention, glutamine (Gln) is included in glutamic acid (Glu).

[0017] (Method for measuring soluble nitrogen index) The soluble nitrogen index (NSI) is measured as follows. Add 60 ml of water to 3 g of the protein material sample, stir with a propeller at 37°C for 1 hour, then centrifuge at 1,400×g for 10 minutes, and collect the supernatant (I). Next, add 100 ml of water again to the remaining precipitate, stir with a propeller at 37°C for 1 hour again, and then centrifuge to collect the supernatant (II). Combine the (I) liquid and the (II) liquid, add water to the mixture to make 250 ml. After filtering this through filter paper (No. 5), measure the nitrogen content in the filtrate by the Kjeldahl method. At the same time, measure the amount of nitrogen in the sample by the Kjeldahl method, and NSI is the ratio of the amount of nitrogen recovered as the filtrate (water-soluble nitrogen) to the total amount of nitrogen in the sample, expressed as mass%. Basically, it is obtained by rounding off the numerical value of the second digit after the decimal point.

[0018] <TCA solubility rate> Add an equal amount of 0.44 M trichloroacetic acid (TCA) to a 2% by mass aqueous solution of the protein material to make a 0.22 M TCA solution, and the value obtained by measuring the ratio of soluble nitrogen by the Kjeldahl method is used. Basically, it is obtained by rounding off the numerical value of the second digit after the decimal point.

[0019] (Method for measuring amino acid composition) The amino acid composition can be calculated according to general amino acid composition analysis methods. Specifically, the protein hydrolysate is measured using an automated amino acid analyzer. 2 mg of the sample is added to 1 ml of 6N hydrochloric acid, hydrolyzed at 110°C for 24 hours, and then the hydrochloric acid is removed under reduced pressure. The sample is dissolved in 10 ml of 0.02N hydrochloric acid and filtered through a 0.45 μm filter, then analyzed using an amino acid analysis system (LA8080 high-speed amino acid analyzer, Hitachi High-Tech Corporation). Glutamine (Gln) is measured as glutamic acid (Glu).

[0020] (Diarrhea-suppressing ingredient) The diarrhea-suppressing material of the present invention suppresses diarrhea and loose stools in dogs and guides the excretion of feces back to a normal state. The dosage used in this case is an oral administration of 0.4 g / kg body weight / day or more as a poorly soluble soy protein material. Preferably, it is 0.45 g / kg body weight / day or more, and more preferably 0.5 g / kg body weight / day or more. Even if the amount is higher, it will not affect the effect, but it will become more difficult to take, so a suitable upper limit for easy intake is preferably 2 g / kg body weight / day or less. More preferably, it is 1.5 g / kg body weight / day or less, and even more preferably 1 g / kg body weight / day or less. There are multiple causes of diarrhea and loose stools in dogs, and often the cause is unclear. However, by implementing this invention, diarrhea and loose stools can be resolved within a few days. Resolving diarrhea and loose stools simplifies cleaning in the dog's living area and when the dog defecates during walks, which is a great benefit to both the owner and the person responsible for cleaning. Furthermore, it reduces the discomfort the dog experiences due to diarrhea, improving the owner's satisfaction.

[0021] (Dog food) The poorly soluble soy protein material of the present invention can be used in dog feed. The content of the poorly soluble soy protein material in the feed dry matter is preferably 0.5% by mass or more, more preferably 1% by mass or more, and most preferably 1.5% by mass or more. When the poorly soluble soy protein material is added to dog food used as a staple food, so-called complete nutritional food, the upper limit is preferably 15% by mass or less, more preferably 10% by mass or less, and most preferably 5% by mass or less. To ensure that the target dogs ingest this material and to allow for fine adjustments according to the dog's weight and food intake, it is preferable to include this material in a highly palatable form of feed, such as dog food used as a supplement. In this case, 8% by mass or more is preferable, 12% by mass or more is more preferable, and 16% by mass or more is most preferable. The upper limit is an amount that dogs do not actively avoid, for example, 60% by mass or less, 55% by mass or less, and 50% by mass or less can be given as examples. As a form of feed that enhances the palatability of dogs, dry feed with a moisture content of less than 25% by mass can be selected, but it is preferable to have a semi-moist type with a moisture content of about 25-40% by mass, a soft-dry type with a moisture content of about 40-75% by mass, or a wet type with a moisture content of 75% or more. It is even more preferable to have a wet type with a moisture content of 75% or more, and wet type feeds with a moisture content of 80% by mass or 85% by mass or more are particularly preferable. Also, it is preferable that the moisture content is 90% by mass or less.

[0022] These feeds containing poorly soluble soy protein are expected to be effective in suppressing diarrhea and loose stools in dogs. High-concentration feeds, in particular, can be administered efficiently. Specific examples of feeds include dog food, diets, or supplements. There are no particular restrictions on their form, but they can be pellets, flakes, pastes, gels, solids, or liquids. Distribution forms include dried feed, refrigerated feed, frozen feed, canned, bottled, aseptically filled, and retort. In addition to dried feed, soft-dry, semi-moist, and wet types can also be prepared, but canned or retort-packaged wet types are particularly preferred.

[0023] (Medicine for dogs) The canine pharmaceutical of the present invention contains a poorly soluble soy protein material that has the effect of suppressing diarrhea and loose stools in dogs as described above. There are no particular restrictions on the form of the canine pharmaceutical, but examples include tablets, powders, granules, and liquids. All are administered orally. [Examples]

[0024] The present invention will be explained below by describing examples. Note that "%" and "parts" in the text refer to "mass%" and "parts by mass," respectively. Furthermore, the indication of g / kg body weight, which represents the dosage per body weight, means g / kg body weight / day.

[0025] (Preparation of poorly soluble protein material) To a 10% by mass aqueous solution of isolated soy protein ("Fujipro-R", manufactured by Fuji Oil Co., Ltd.), 0.2% by mass of protease ("Protin SD-AY10", manufactured by Amano Enzyme Co., Ltd.) was added, and an enzymatic decomposition reaction was carried out at 50°C for 5 hours. After the reaction, the solution was heated at 100°C for 5 minutes to inactivate the enzyme, and then cooled to room temperature. The precipitate was collected by centrifugation at 8,000 G for 10 minutes to obtain a poorly soluble fraction (HMF), which is a poorly digestible and poorly soluble soy protein material. The obtained HMF was freeze-dried and then used in subsequent processes. Table 1 shows the analytical values ​​for HMF. The amino acid composition of HMF was Glu 11.5% by mass, Leu 11.0% by mass, and the Glu / Leu ratio was 1.05. The NSI was 48.9%, and the TCA solubilization rate was 32.5%. The analytical values ​​for "Fujipro-R" are also shown as an example of undegraded isolated soy protein material (SPI).

[0026] Table 1: Material Analysis Values TIFF2026049151000001.tif140111

[0027] (Animal testing) For regular diets, we used Regular Diet A ("Medicote Allergen-Cut Fish & Rice (for dogs 11 years and older)" manufactured by Petline Co., Ltd.) and Regular Diet B ("Super Gold Breeder Pack for Senior Dogs" manufactured by Morinaga Sunworld Co., Ltd.). For supplemental wet feed, we used "Grand Deli Domestic Chicken Breast Pouch for Adult Dogs, Shredded, with Green and Yellow Vegetables and Cheese" manufactured by Unicharm Corporation, with a moisture content of 86.4% by mass.

[0028] As shown in Table 2, five healthy dogs with an average weight of 4.32 kg were fed their regular diet for two weeks as a preliminary treatment. Afterward, they were given 35 g (Wet) of supplemental wet feed per dog per day in addition to their regular diet for seven days (regular diet period: day 1 to day 7). Next, 35g (Wet) of supplemental wet feed per animal per day and 0.5g / kg body weight per day of test HMF were added to the regular diet and given for another 14 days (test diet period: day 8 to day 21).

[0029] Table 2: Test animals and their regular diet TIFF2026049151000002.tif39116

[0030] Table 3 shows the characteristics of the stool over a total of 21 days: 7 days during the regular diet period and 14 days during the test diet period containing HMF. Stool consistency records showed improvement in loose stools. After the 4th day (day 11) of the test diet period (HMF intake), no individuals exhibited loose stools, and all individuals improved to normal stools. Specifically, individual number 2 exhibited loose stools every other day, but after the 3rd day (day 10) of HMF intake, all stools were normal. Individuals number 3 and 5 each exhibited loose stools once, but after HMF intake, all stools were normal. Individual number 4 exhibited loose stools for 3 consecutive days, but after HMF intake (day 8), all stools were normal. Individual number 1 had normal stools before HMF intake, and after HMF intake (day 8), it did not experience constipation and had normal stools every day. All individuals had bowel movements every day without constipation.

[0031] Table 3: Stool characteristics during the test diet period TIFF2026049151000003.tif148122

[0032] As described above, it was confirmed that dogs that ingested 0.4g / kg / day of HMF experienced a resolution of loose stools. [Industrial applicability]

[0033] This invention makes it possible to widely distribute dog food and other feeds that suppress diarrhea or loose stools in dogs. Furthermore, this material is a common by-product of the industrial production of soy peptides, leading to the effective utilization of by-products.

Claims

1. A diarrhea-suppressing ingredient for dogs, containing a poorly soluble soy protein material as the active ingredient, having an amino acid composition with a mass ratio of glutamic acid (Glu) to leucine (Leu) of 2 or less and an NSI of 10-60%.

2. The diarrhea-suppressing material for dogs according to claim 1, wherein the poorly soluble soy protein material is an undegraded residue produced by enzymatic hydrolysis of a soy protein raw material.

3. A diarrhea-suppressing material for dogs according to claim 1 or 2, for oral administration of 0.4 g / kg / day or more of poorly soluble soy protein material.

4. A method for producing a diarrhea-suppressing material for dogs, comprising enzymatic hydrolysis of a soy protein raw material and recovery of the resulting undegraded residue as a poorly soluble soy protein material.

5. A method for producing a diarrhea-suppressing material for dogs according to claim 4, wherein the poorly soluble soy protein material has an amino acid composition in which the mass ratio of glutamic acid (Glu) to leucine (Leu) is 2 or less, and the NSI is 10 to 60%.

6. A method for producing a diarrhea-suppressing material for dogs according to claim 4 or 5, for oral administration of 0.4 g / kg / day or more of poorly soluble soy protein material.

7. A method for suppressing diarrhea in dogs, using a poorly soluble soy protein material having an amino acid composition in which the mass ratio of glutamic acid (Glu) to leucine (Leu) is 2 or less, and the NSI is 10-60%.

8. A method for suppressing diarrhea in dogs according to claim 7, comprising orally administering 0.4 g / kg / day or more of poorly soluble soy protein material.

9. A method for suppressing diarrhea in dogs according to claim 7 or claim 8, wherein the poorly soluble soy protein is an undegraded residue produced by enzymatic hydrolysis of a soy protein raw material.

10. Dog feed containing poorly soluble soy protein material, having an amino acid composition with a mass ratio of glutamic acid (Glu) to leucine (Leu) of 2 or less, and an NSI of 10-60%.

11. The dog feed according to claim 10, wherein the poorly soluble soy protein material is an undegraded residue produced by enzymatic hydrolysis of a soy protein raw material.

12. Dog feed according to claim 10 or claim 11, comprising 0.5% by mass or more of poorly soluble soy protein material in dry matter.

13. A dog feed according to claim 10 or claim 11, which is a wet-type feed with a moisture content of 75% by mass or more, and contains 8% by mass or more of poorly soluble soy protein material in its dry matter.

14. A pharmaceutical product for suppressing diarrhea in dogs, containing a poorly soluble soy protein material as the active ingredient, having an amino acid composition with a mass ratio of glutamic acid (Glu) to leucine (Leu) of 2 or less, and an NSI of 10-60%.

15. A pharmaceutical product for suppressing diarrhea in dogs, according to claim 14, wherein the poorly soluble soy protein material is an undegraded residue produced by enzymatic hydrolysis of a soy protein raw material.

16. A pharmaceutical product for suppressing diarrhea in dogs, according to claim 14 or claim 15, for oral administration of 0.4 g / kg body weight / day or more as a poorly soluble soy protein material.

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