Ultrasonic device and method of use
The ultrasonic device addresses the issue of air bubbles in 3D cell culture by using cavitation to remove trapped bubbles mechanically, ensuring uniform distribution and aggregation of spheroids and organoids without vessel damage or additional processing steps.
JP2026094139APending Publication Date: 2026-06-09CORNING INC
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- CORNING INC
- Filing Date
- 2026-02-02
- Publication Date
- 2026-06-09
AI Technical Summary
Technical Problem
The generation of spheroids and organoids in 3D cell culture is hindered by air bubbles entering the wells, preventing uniform distribution and aggregation, and existing methods like manual stirring or chemical treatments can damage the vessel or require additional steps.
Method used
An ultrasonic device with a piezoelectric transducer generates vibrations to induce cavitation, mechanically removing trapped bubbles from microcavity wells without damaging the cell culture vessel or requiring special packaging.
Benefits of technology
The ultrasonic device efficiently removes bubbles, simplifying the process, reducing handling time, and maintaining the aseptic environment, while allowing for uniform cell seeding and aggregation formation.
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Smart Images
Figure 2026094139000001_ABST
Abstract
This invention provides an ultrasonic device and method for mechanically removing trapped air bubbles from cell culture vessels. [Solution] The ultrasonic device comprises a housing having an ultrasonic surface, an ultrasonic transducer, and an ultrasonic horn that delivers and focuses energy from the ultrasonic transducer to the ultrasonic surface. A method for removing trapped bubbles from a cell culture vessel includes positioning the cell culture vessel, which contains trapped bubbles in the liquid within the microcavity well of the cell culture vessel, to the ultrasonic surface of the ultrasonic device. Mechanical agitation is generated by the ultrasonic device and applied to the cell culture vessel to remove trapped bubbles from the microcavity well. A method for releasing cell aggregates from a cell culture vessel includes positioning the cell culture vessel, which contains cell aggregates in the microcavity well, to the ultrasonic surface of the ultrasonic device. By applying mechanical agitation from the ultrasonic device to the surface of the cell culture vessel, the cell aggregates are released from the microcavity well.
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